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KONDO Masato

■FacultyFaculty Division of Natural Sciences Research Group of Chemistry
■PositionAssociate Professor
Last Updated :2024/06/12

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Profile and Settings

  • Name (Japanese)

    Kondo

  • Name (Kana)

    Masato

Degree

  • Ph.D, Kyoto University, Mar. 2011

Research Interests

  • 分子拡散
  • 界面
  • 過渡回折格子法
  • 振動分光

Research Areas

  • Nanotechnology/Materials, Basic physical chemistry

Research Experience

  • May 2024, 9999, Nara Women's University, 自然科学系化学領域, 准教授
  • Oct. 2015, Apr. 2024, University of Tsukuba, 数理物質系化学域, 助教
  • Apr. 2014, Oct. 2015, Osaka University, Graduate School of Science Department of Chemistry, 特任研究員
  • Apr. 2011, Mar. 2014, Japan Atomic Energy Agency, 量子ビーム応用研究部門, Postdoctoral fellow

Education

  • Apr. 2007, Mar. 2011, Kyoto University, Graduate School of Science, Division of Chemistry, 博士後期課程
  • Apr. 2005, Mar. 2007, Kyoto University, Graduate School of Science, Division of Chemistry, 修士課程
  • Apr. 2001, Mar. 2005, Kyoto University, Faculty of Science, Faculty of Science

Association Memberships

  • The Society for Molecular Science, Jun. 2008, 9999
  • The Chemical Society of Japan, Jan. 2008, 9999
  • The Spectroscopical Society of Japan, Mar. 2007, 9999
  • The Japanese Photochemistry Association, May 2008, Dec. 2022

Ⅱ．研究活動実績

Published Papers

  • Refereed, Journal of Physical Organic Chemistry, Wiley, Substituted effects on bonding characteristics of cyclopentane‐1,3‐diyl diradicals monitored by time‐resolved infrared spectroscopy, Masato Kondoh; Shunsuke Kuboki; Hidetaka Kume; Eriku Oda; Manabu Abe; Taka‐aki Ishibashi, Abstract Cyclopentane‐1,3‐diyl diradicals (DRs) provide excellent opportunities to study the properties of diradicals because their lifetimes can be significantly lengthened to up to milliseconds with the introduction of proper substituents. This study investigated the bonding characteristics of singlet and triplet DRs having C=O and p‐cyanophenyl groups (S‐DR3 and T‐DR3) by monitoring the photo‐induced formation of the diradicals from their precursor azo compounds using time‐resolved IR (TR‐IR) spectroscopy. Upon the formation of S‐DR3, a C=O stretching wavenumber was upshifted by 22 cm−1, whereas a C≡N stretching one was downshifted by 12 cm−1. The observed shifts indicate that the unpaired electrons increase and decrease the C=O and C≡N bond orders, respectively. The effects of the unpaired electrons in S‐DR3 were similar to those observed in our previous TR‐IR studies on a singlet cyclopentane‐1,3‐diyl diradical having C=O but no C≡N groups (S‐DR2) and on that having C≡N but no C=O groups (S‐DR1), respectively. Contrastingly, upon the formation of T‐DR3, the C=O wavenumber was downshifted by 16 cm−1, indicating that the unpaired electrons decrease the C=O bond order. More notably, no detectable shifts were observed in the C≡N stretching wavenumber. These observations are not clearly explained by a model suggested in the previous studies on S‐DRs. Here, we discuss and propose a more elaborated resonance hybrid of DRs that can explain the directions and relative magnitudes of the observed wavenumber shifts irrespective of spin multiplicities. We expect that the findings and suggestions presented here will stimulate research in both organic and theoretical chemistry., 11 Oct. 2023, 37, 2, Scientific journal, 10.1002/poc.4575
    PermalinkDOI URL
  • Refereed, Accounts of Materials & Surface Research, Photoinduced ring opening and closure reactions in chalcones monitored by time-resolved IR spectroscopy, Masato Kondoh; Taka-aki Ishibashi, Apr. 2023, 8, 1, 46, 57, Scientific journal
  • Refereed, The Journal of Physical Chemistry B, American Chemical Society (ACS), Total Internal Reflection Raman Spectra of Alamethicin Interacting with Supported Lipid Bilayers at a Silica/Water Interface, Masato Kondoh; Arisa Sano; Izuru Kawamura; Taka-aki Ishibashi, 28 Nov. 2022, 126, 50, 10712, 10720, Scientific journal, 10.1021/acs.jpcb.2c06371
    PermalinkDOI URL
  • JOURNAL OF PHYSICAL CHEMISTRY B, AMER CHEMICAL SOC, Time-Resolved Infrared Spectroscopy with Multivariate Analysis on Photoinduced Proton Transfer in Aromatic Urea-Acetate Anion Complexes, Masato Kondoh; Hayato Honda; Kei Togasaki; Yoshinobu Nishimura; Tatsuo Arai; Taka-aki Ishibashi, 1-Anthracen-2-yl-3-phenylurea (2PUA) is an aromatic urea compound, which forms a hydrogen-bonded complex with an acetate anion (AcO-), 2PUA-AcO- complex. We investigated the photoinduced reaction of the 2PUA-AcO- complex in dimethyl sulfoxide (DMSO) by nanosecond time-resolved infrared (TR-IR) spectroscopy. TR-IR spectra obtained after the photoexcitation of 2PUA with the equal concentration of AcO- were consistently explained by a photoinduced proton transfer model. The spectral and temporal profiles of the TR-IR spectra largely depended on concentration conditions of 2PUA and AcO-. Under the condition where excessive amounts of AcO- existed, the TR-IR spectra contained an unexpected signal whose amplitude was related to the concentration of free AcO- in the solution. Using singular value decomposition analysis of the concentration-dependent TR-IR spectra, we extracted the spectral component that reflects the photoinduced reaction of the 2PUA-AcO- complex. The extracted spectrum resembled the TR-IR spectrum obtained under the equal concentration condition, indicating that the same proton transfer occurs during the photoinduced reaction of the 2PUA-AcO- complex irrespective of the concentration conditions. Comparing the steady-state and transient IR spectra of the 2PUA with AcO- in DMSO with density functional theory calculations suggests that both 2PUA-AcO- complex and tautomer species interact with solvent DMSO molecules in their electronic ground states to a large extent., Feb. 2022, 126, 4, 912, 921, Scientific journal, 10.1021/acs.jpcb.1c09526
    DOI URL
  • Not Refereed, JOURNAL OF PHYSICAL CHEMISTRY LETTERS, AMER CHEMICAL SOC, Quadrupole Contribution of C=O Vibrational Band in Sum Frequency Generation Spectra of Organic Carbonates, Wang, Lin; Mori, Wataru; Morita, Akihiro; Kondoh, Masato; Okuno, Masanari; Ishibashi, Taka-aki, Sum Frequency Generation (SFG) is usually governed by surface-selective signals of dipole origin, but it can also contain some bulk signals of quadrupole origin. In this work, we examined the dipole and quadrupole contributions in the C=O stretching band of organic carbonate liquids with collaboration of heterodyne SFG measurement and theoretical analysis. As a result, we found that these spectra are substantially affected by the quadrupole contribution of the bulk, which resolved the discrepancy between the experimental and computational SFG spectra., Oct. 2020, 11, 20, 8527, 8531, Scientific journal, 10.1021/acs.jpclett.0c02453
    PermalinkDOI URL
  • Refereed, Bulletin of the Chemical Society of Japan, The Chemical Society of Japan, Observation of translational diffusion in a planer supported lipid bilayer membrane by total internal reflection-transient grating method, Kondoh, Masato; Moritani, Hidekazu; Ishibashi, Taka-aki, Total internal reflection-transient grating (TIR-TG) method has been applied to diffusion coefficient measurements of trans-stilbene in a dimyristoylphosphatidylcholine (DMPC) lipid bilayer membrane at a silica/water interface. The observed TIR-TG signal consisted of four decay components. The rate constants of the third and fourth components quadratically depended on grating wavenumbers, which indicates that these components represent diffusion processes of trans-stilbene (diffusion signals). From the decay rate constants of the diffusion signals, two different diffusion coefficients, (5.7 +/- 1.0) x 10(-)(10) and (2.8 +/- 0.5) x 10(-12) m(2)s(-1) were obtained for trans-stilbene in the DMPC membrane. This result indicates that the DMPC membrane is an inhomogeneous environment that can be described with two different effective viscosities., May 2020, 93, 5, 671, 675, Scientific journal, 10.1246/bcsj.20200019
    DOI URL
  • Refereed, Journal of Photochemistry and Photobiology A: Chemistry, ELSEVIER SCIENCE SA, Time-resolved infrared study of photo-induced ring-closure reaction of trans-2-hydroxychalcone, Kondoh, Masato; Takizawa, Chinatsu; Okazawa, Kazuki; Akase, Dai; Aida, Misako; Ishibashi, Taka-aki, The photo-induced ring-closure reaction from trans-2-hydroxychalcone (Ct) to flav-3-en-2-ol (F) in acetonitrile was studied by time-resolved infrared (TR-IR) spectroscopy. TR-IR difference spectra observed immediately after photoexcitation of Ct contained negative peaks at 1604 and 1664 cm(-1) where Ct has vibrational bands. Their amplitudes became more negative with a common time constant of 10 ms, which indicates that intermediate species generated within the time-resolution also had vibrational bands at the wavenumbers. We analyzed the observed TR-IR difference spectra assuming that the spectra consist of three spectral components due to Ct, F, and an intermediate and extracted the vibrational spectrum of the intermediate by subtracting contributions of Ct and F from the observed difference spectra. Based on vibrational analysis by density functional theory calculations, the intermediate of the photo-ring-closure reaction was assigned to cis-2-hydroxychalcone hydrogen-bonded to the solvent molecules., Dec. 2019, 389, 112280, Scientific journal, 10.1016/j.jphotochem.2019.112280
    DOI URL
  • The Journal of Physical Chemistry B, American Chemical Society (ACS), Photo-Induced Ring-Opening Reaction of Flav-3-en-2-ol Monitored by Time-Resolved Infrared Spectroscopy, Masato Kondoh; Akira Sakuta; Kazuki Okazawa; Dai Akase; Misako Aida; Taka-aki Ishibashi, 27 Sep. 2019, 123, 40, 8499, 8504, Scientific journal, 10.1021/acs.jpcb.9b07108
    PermalinkDOI URL
  • Not Refereed, PHOTOCHEMISTRY AND PHOTOBIOLOGY, WILEY, Conformational and Intermolecular Interaction Dynamics of Photolyase/Cryptochrome Proteins Monitored by the Time-Resolved Diffusion Technique, Masato Kondoh; Masahide Terazima, Cryptochrome (CRY), a blue light sensor protein, possesses a similar domain structure to photolyase (PHR) that, upon absorption of light, repairs DNA damage. In this review, we compare the reaction dynamics of these systems by monitoring the reaction kinetics of conformational change and intermolecular interaction change based on time-dependent diffusion coefficient measurements obtained by using the pulsed laser-induced transient grating technique. Using this method, time-dependent biomolecular interactions, such as transient dissociation reactions in solution, have been successfully detected in real time. Conformational change in (6-4) PHR has not been detected after the photoexcitation by monitoring the diffusion coefficient. However, the repaired DNA dissociates from PHR with a time constant of 50 mu s, which must relate to a minor conformational change. However, CRY exhibits a considerable diffusion change with a time constant of 400 ms, which indicates that the protein-solvent interaction is changed by the conformational change. The C-terminal domain of CRY is shown to be responsible for this change., Jan. 2017, 93, 1, 15, 25, Scientific journal, 10.1111/php.12681
    DOI URL
  • The Journal of Physical Chemistry Letters, American Chemical Society (ACS), Importance of Atomic Contacts in Vibrational Energy Flow in Proteins, Masato Kondoh; Misao Mizuno; Yasuhisa Mizutani, 12 May 2016, 7, 11, 1950, 1954, Scientific journal, 10.1021/acs.jpclett.6b00785
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  • OPTICS EXPRESS, OPTICAL SOC AMER, Liquid-sheet jets for terahertz spectroscopy, Masato Kondoh; Masaaki Tsubouchi, We investigated liquid-sheet jets with controllable thickness for application to terahertz (THz) spectroscopy. Slit-type and colliding-jet nozzles were used to generate optically flat liquid jets. The thickness of the liquid sheet was determined precisely by spectral interference and THz time-domain-spectroscopy methods. By adjusting the collision angle of the colliding-jet nozzle, we could control the thickness of the liquid sheet from 50 to 120 mu m. (C)2014 Optical Society of America, Jun. 2014, 22, 12, 14135, 14147, Scientific journal, 10.1364/OE.22.014135
    DOI URL
  • CHEMICAL PHYSICS LETTERS, ELSEVIER SCIENCE BV, Ion effects on the structure of water studied by terahertz time-domain spectroscopy, Masato Kondoh; Yasuhiro Ohshima; Masaaki Tsubouchi, We have investigated dielectric relaxation in aqueous ionic solutions by terahertz time-domain spectroscopy to elucidate the ionic hydration effect on the water structure. In the low frequency region (<1.0 THz), the dielectric spectra decreased in amplitude with decreasing size of dissolved cations, which indicates the deceleration effect of water within the hydration shell. On the other hand, in the high frequency region (1.0-1.8 THz), the spectra increased in amplitude as compared to that of pure water. This finding indicates dissolved ions weaken the hydrogen bonding of water in ionic solution and induce the structure breaking effect beyond the hydration shell. (C) 2013 Elsevier B. V. All rights reserved., Jan. 2014, 591, 317, 322, Scientific journal, 10.1016/j.cplett.2013.11.055
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  • 2013 38TH INTERNATIONAL CONFERENCE ON INFRARED, MILLIMETER, AND TERAHERTZ WAVES (IRMMW-THZ), IEEE, Ion Effects on Liquid Structure of Water Monitored by Terahertz Time-Domain Spectroscopy, Masato Kondoh; Yasuhiro Ohshima; Masaaki Tsubouchi, We have investigated dielectric relaxation in aqueous ionic solutions by terahertz time-domain spectroscopy to elucidate the ionic hydration effect on the structure of water. Our finding suggests the dissolved ion induces the "structure breaking effect" which weakens the hydrogen bonding of water beyond the ion hydration shell., 2013, International conference proceedings
  • JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Light-Induced Conformational Changes in Full-Length Arabidopsis thaliana Cryptochrome, Masato Kondoh; Chiaki Shiraishi; Pavel Mueller; Margaret Ahmad; Kenichi Hitomi; Elizabeth D. Getzoff; Masahide Terazima, Cryptochromes (CRYs) are widespread flavoproteins with homology to photolyases (PHRs), a class of blue-light-activated DNA repair enzymes. Unlike PHRs, both plant and animal CRYs have a C-terminal domain. This cryptochrome C-terminal (CCT) domain mediates interactions with other proteins, while the PHR-like domain converts light energy into a signal via reduction and radical formation of the flavin adenine dinucleotide cofactor. However, the mechanism by which the PHR-like domain regulates the CCT domain is not known. Here, we applied the pulsed-laser-induced transient grating method to detect conformational changes induced by blue-light excitation of full-length Arabidopsis thaliana cryptochrome 1 (AtCRY1). A significant reduction in the diffusion coefficient of AtCRY1 was observed upon photoexcitation, indicating that a large conformational change occurs in this monomeric protein. AtCRY1 containing a single mutation (W324F) that abolishes an intra-protein electron transfer cascade did not exhibit this conformational change. Moreover, the conformational change was much reduced in protein lacking the CCT domain. Thus, we conclude that the observed large conformational changes triggered by light excitation of the PHR-like domain result from C-terminal domain rearrangement. This inter-domain modulation would be critical for CRYs' ability to transduce a blue-light signal into altered protein protein interactions for biological activity. Lastly, we demonstrate that the transient grating technique provides a powerful method for the direct observation and understanding of photoreceptor dynamics. (C) 2011 Elsevier Ltd. All rights reserved., Oct. 2011, 413, 1, 128, 137, Scientific journal, 10.1016/j.jmb.2011.08.031
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  • JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, Transient Dissociation of the Transducer Protein from Anabaena Sensory Rhodopsin Concomitant with Formation of the M State Produced upon Photoactivation, Masato Kondoh; Keiichi Inoue; Jun Sasaki; John L. Spudich; Masahide Terazime, Anabaena sensory rhodopsin (ASR), a microbial rhodopsin in the cyanobacterium sp. PCC7120, has been suggested to regulate cell processes in a light-quality-dependent manner (color-discrimination) through interaction with a water-soluble transducer protein (Tr). However, light-dependent ASR-Tr interaction changes have yet to be demonstrated. We applied the transient grating (TG) method to investigate protein-protein interaction between ASR with Tr. The molecular diffusion component of the TG signal upon photostimulation of ASR(AT) (ASR with an all-trans retinylidene chromophore) revealed that Tr dissociates from ASR upon formation of the M-intermediate and rebinds to ASR during the decay of M; that is, light induces transient dissociation of ASR and Tr during the photocycle. Further correlating the dissociation of the ASR-Tr pair with the M-intermediate, no transient dissociation was observed after the photoexcitation of the blue-shifted ASR(13C) (ASR with 13-cis, 15-syn chromophore), which does not produce M. This distinction between ASR(AT) and ASR(13C), the two isomeric forms in a color-sensitive equilibrium in ASR, provides a potential mechanism for color-sensitive signaling by ASR., Aug. 2011, 133, 34, 13406, 13412, Scientific journal, 10.1021/ja202329u
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  • JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, Light-Induced Conformational Change and Product Release in DNA Repair by (6-4) Photolyase, Masato Kondoh; Kenichi Hitomi; Junpei Yamamoto; Takeshi Todo; Shigenori Iwai; Elizabeth D. Getzoff; Masahide Terazima, Proteins of the cryptochrome/photolyase family share high sequence similarities, common folds, and the flavin adenine dinucleotide (FAD) cofactor, but exhibit diverse physiological functions. Mammalian cryptochromes are essential regulatory components of the 24 h circadian clock, whereas (6-4) photolyases recognize and repair UV-induced DNA damage by using light energy absorbed by FAD. Despite increasing knowledge about physiological functions from genetic analyses, the molecular mechanisms and conformational dynamics involved in clock signaling and DNA repair remain poorly understood. The (6-4) photolyase, which has strikingly high similarity to human clock cryptochromes, is a prototypic biological system to study conformational dynamics of cryptochrome/photolyase family proteins. The entire light-dependent DNA repair process for (6-4) photolyase can be reproduced in a simple in vitro system. To decipher pivotal reactions of the common FAD cofactor, we accomplished time-resolved measurements of radical formation, diffusion, and protein conformational changes during light-dependent repair by full-length (6-4) photolyase on DNA carrying a single UV-induced damage. The (6-4) photolyase by itself showed significant volume changes after blue-light activation, indicating protein conformational changes distant from the Flavin cofactor. A drastic diffusion change was observed only in the presence of both (6-4) photolyase and damaged DNA, and not for (6-4) photolyase alone or with undamaged DNA. Thus, we propose that this diffusion change reflects the rapid (50 mu s time constant) dissociation of the protein from the repaired DNA product. Conformational changes with such fast turnover would likely enable DNA repair photolyases to access the entire genome in cells., Feb. 2011, 133, 7, 2183, 2191, Scientific journal, 10.1021/ja107691w
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  • CHEMICAL PHYSICS LETTERS, ELSEVIER SCIENCE BV, Photochemical reaction and diffusion of caged calcium studied by the transient grating, Masato Kondoh; Naoki Baden; Masahide Terazima, A photoreaction of caged calcium (Ca(2+)) was investigated by using the time-resolved transient grating (TG) method. The q(2)- dependent feature of the TG signal was analyzed based on a model that there are two parallel dissociation steps with different rates and Ca(2+) is released promptly after the fracture of the caged compound. The TG signal representing the presence of Ca(2+) was appeared by the volume contribution. Although the diffusion coefficients of Cg and the decomposed product are different without Ca(2+), only one diffusion component was observed after the dissociation of CgCa(2+), indicating that the caged compounds and Ca(2+) diffuse together. (C) 2008 Elsevier B. V. All rights reserved., Sep. 2008, 462, 1-3, 58, 63, Scientific journal, 10.1016/j.cplett.2008.07.032
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MISC

  • 工作ニュース, <研究ノート>脂質膜と生体分子間相互作用観測のための全内部反射ラマン分光装置の構築, 近藤正人; 佐野有里紗; 石橋孝章, Apr. 2021, 12, 7, 11, Report research institution

Presentations

  • Takumi Kameyama; Masato Kondoh; Atsuya Momotake; Taka-aki Ishibashi, 9th SFG symposium, Measurement of HD-VSFG spectra of SHG active dye Ap3 in lipid monolayers on water, 13 Nov. 2023
  • 鈴木沙弥; 近藤正人; 石橋孝章, 第17回分子科学討論会, 全内部反射ラマン分光によるグラミシジンAと基板支持脂質二分子膜との相互作用の観測, 14 Sep. 2023
  • 亀山拓海; 近藤正人; 百武篤也; 石橋孝章, 第17回分子科学討論会, SHG活性色素Ap3の水上脂質単分子膜中におけるHD-VSFGスペクトル測定, 12 Sep. 2023
  • 篠田亮介; 近藤正人; 百武篤也; 石橋孝章, 第17回分子科学討論会, HD-VSFG分光法による空気/水溶液界面の脂質単分子膜と水溶性色素の相互作用の観測, 12 Sep. 2023
  • 近藤正人; 窪木俊介; 久米英隆; 小田英里久; 安倍学; 石橋孝章, 第17回分子科学討論会, 時間分解赤外分光で観るシクロペンタン-1,3-ジラジカルの結合性に対する置換基効果, 12 Sep. 2023
  • 鈴木, 沙弥; 近藤, 正人; 石橋, 孝章, 分子科学討論会, リン脂質二分子膜中のグラミシジンAの全内部反射ラマンスペクトル測定, 19 Sep. 2022, 22 Sep. 2022
  • 篠田, 亮介; 近藤, 正人; 百武 篤也; 石橋, 孝章, 分子科学討論会, 4'-ジヘキシルアミノ-4-スルホアゾベンゼン水溶液上の脂質単分子膜のHD-VSFG分光, 19 Sep. 2022, 22 Sep. 2022
  • 近藤正人; 佐野有里沙; 鈴木沙弥; 石橋孝章, 第16回分子科学討論会, 全内部反射ラマン分光による膜結合ペプチドと基板支持脂質二分子膜の相互作用の観測, Sep. 2022
  • Kondoh, Masato; Ishibashi, Taka-aki, The Pacifichem 2021 meeting, Time-resolved IR spectroscopy of photo-induced ring opening and closure reactions between 2-hydroxychalcone and flav-3-en-2-ol, 21 Dec. 2021, 21 Dec. 2021, True
  • 佐野, 有里紗; 近藤, 正人; 川村出; 石橋, 孝章, 日本分光学会年次講演会, 全内部反射ラマン分光による固液界面リン脂質二重膜とアラメチシンの相互作用観測の試み, 13 Oct. 2021, 13 Oct. 2021, False
  • 佐野, 有里紗; 近藤, 正人; 川村, 出; 石橋, 孝章, 分子科学討論会, 固液界面におけるリン脂質二重膜と膜透過ペプチドの全内部反射ラマンスペクトル測定, 21 Sep. 2021, 21 Sep. 2021, False
  • 近藤, 正人; 新井, 達郎; 中島, 晃希; 石橋, 孝章, 分子科学討論会, VSFG分光で観るSHG活性色素Ap3の平面支持脂質二重膜への吸脱着過程, 21 Sep. 2021, 21 Sep. 2021, False
  • 永山, 弘季; 近藤, 正人; 田島, 優衣; 石橋, 孝章, 分子科学討論会, 非光反応性タンパク質の紫外光励起過渡回折格子信号とその起源の検討, 19 Sep. 2021, 19 Sep. 2021, False
  • 中島, 晃希; 近藤, 正人; 石橋, 孝章, 日本分光学会年次講演会, HD-VSFG分光法で観るSHGプローブ色素AP3と水上脂質単分子膜の相互作用, 26 Oct. 2020, 26 Oct. 2020, 28 Oct. 2020, 日本分光学会, False
  • 筑波, 標; 近藤, 正人; 石橋, 孝章, 日本分光学会年次講演会, HD-VSFG分光で観る脂質二重膜の構造に対するコレステロールの影響, 26 Oct. 2020, 26 Oct. 2020, 28 Oct. 2020, 日本分光学会, False
  • 近藤, 正人; 田島, 優衣; 永山, 弘季; 石橋, 孝章, 日本分光学会年次講演会, 非光反応性タンパク質水溶液の紫外光励起過渡回折格子信号, 26 Oct. 2020, 26 Oct. 2020, 28 Oct. 2020, 日本分光学会, False
  • 佐野, 有里紗; 近藤, 正人; 江波, 静夏; 石橋, 孝章, 日本分光学会年次講演会, HD-VSFG分光で観る脂質二重膜の構造に対するコレステロールの影響, 26 Oct. 2020, 日本分光学会, False
  • 近藤, 正人; 本多, 駿斗; 東ヶ崎, 慶; 西村, 賢宣; 新井, 達郎; 石橋, 孝章, 第13回分子科学討論会, 芳香族ウレア－酢酸イオン会合体の光誘起プロトン移動の時間分解赤外分光と多変量解析, 17 Sep. 2019, 17 Sep. 2019, 21 Sep. 2019, False
  • 森谷, 英和; 近藤, 正人; 石橋, 孝章, 第13回分子科学討論会, 全内部反射過渡回折格子法による脂質二重膜中の分子拡散係数の測定, 17 Sep. 2019, 17 Sep. 2019, 21 Sep. 2019, False
  • Moritani, Hidekazu; Kondoh, Masato; Ishibashi, Taka-aki, MANA International Symposium, Measurement of molecular diffusion coefficient and reaction rate constant at solid-liquid interface by total internal reflection - transient grating, 04 Mar. 2019, 04 Mar. 2019, 06 Mar. 2019, Tsukuba, False
  • 森渉; 近藤正人; 奥野, 将成; 王琳; 石橋孝章; 森田明弘, 第12回分子科学討論会, Investigation of bulk quadrupole effect in sum frequency generation spectroscopy, 10 Sep. 2018, 10 Sep. 2018, 13 Sep. 2018, 福岡, False
  • 近藤, 正人; 作田, 光; 岡澤, 一樹; 赤瀬, 大; 相田, 美砂子; 石橋, 孝章, The 12th Annual Meeting of Japan Society for Molecular Science, Photochromic reaction of hydroxychalcone and flavenol monitored by time-resolved infrared spectroscopy, 10 Sep. 2018, 10 Sep. 2018, 13 Sep. 2018, 分子化学会, 日本 福岡県博多市, Photochromic reaction of hydroxychalcone and flavenol has been studied by time-resolved infrared (TR-IR) spectroscopy and quantum chemical calculations. Here, the photo-induced ring-opening reaction from flav-3-en-2-ol to trans-2-hydroxychalcone is discussed. A vibrational band due to a C=O stretching mode of an intermediate species, enol form of 2-hydroxychalcone, was detected at 1632 cm-1 in TR-IR spectra obtained after photoexcitation of flav-3-en-2-ol. We also found that a vibrational band at 1664 cm-1, where a C=O stretching mode of trans-2-hydroxychalcone appears, appeared immediately after the photoexcitation and increased in intensity upon depletion of the enol intermediate. The DFT calculation showed that the enol intermediate has no vibrational modes at around the 1664 cm-1. These findings indicate that trans-2-hydroxychalcone is produced not only via the enol intermediate but also directly from flav-3-en2-ol without via the enol intermediate., False
  • 森谷, 英和; 近藤, 正人; 石橋, 孝章, 第12回分子科学討論会, 全内部反射過渡回折格子法による固液界面近傍の分子拡散係数や反応速度定数の測定, 10 Sep. 2018, 10 Sep. 2018, 13 Sep. 2018, False
  • 近藤, 正人; 本多, 駿斗; 東ヶ崎, 慶; 西村, 賢宣; 新井, 達郎; 石橋, 孝章, 第12回分子科学討論会, 時間分解赤外分光で観る芳香族ウレア-アセテートアニオン会合体の光誘起プロトン移動, 10 Sep. 2018, 10 Sep. 2018, 13 Sep. 2018, False
  • 森谷, 英和; 近藤, 正人; 石橋, 孝章, 平成30年度 日本分光学会年次講演会, 固液界面における分子拡散や化学反応を測定するための全内部反射過渡回折格子装置の製作, 22 May 2018, 22 May 2018, 25 May 2018, False
  • 近藤正人; Okuno, Masanari; 井上圭一; 神取秀樹; 石橋孝章, 第11回分子科学討論会, Dynamics of conformational and orientational change in sodium pump rhodopsin at the air/water interface monitored by vibrational sum frequency generation spectroscopy, 15 Sep. 2017, 15 Sep. 2017, 18 Sep. 2017, 仙台, False
  • 本多, 駿斗; 近藤, 正人; 東ヶ崎, 慶; 西村, 賢宣; 新井, 達郎; 石橋, 孝章, 第11回分子科学討論会, 時間分解赤外分光による1-anthracen-2-yl-3-phenylurea-酢酸イオン会合体の光誘起プロトン移動の研究, 15 Sep. 2017, 15 Sep. 2017, 18 Sep. 2017, False
  • 山下, 聡; 近藤, 正人; 水野, 操; 水谷, 泰久, 第11回分子科学討論会, タンパク質における振動エネルギー移動経路, 15 Sep. 2017, 15 Sep. 2017, 18 Sep. 2017, False
  • 近藤, 正人; 井上圭一; 神取秀樹; 石橋, 孝章, 平成29年度 日本分光学会年次講演会, 気水界面におけるナトリウムポンプロドプシンの振動和周波発生分光, 23 May 2017, 23 May 2017, 25 May 2017, False
  • 本多, 駿斗; 近藤, 正人; 東ヶ崎, 慶; 西村, 賢宣; 新井, 達郎; 石橋, 孝章, 平成29年度 日本分光学会年次講演会, 時間分解赤外分光による1-anthracen-2-yl-3-phenylurea-酢酸イオン会合体の光励起プロトン移動の研究, 23 May 2017, 23 May 2017, 25 May 2017, False
  • 近藤正人, 日本分光学会 先端レーザー分光部会 第11回若手研究者による先端的レーザー分光シンポジウム, 紫外共鳴ラマン分光法によるタンパク質内振動エネルギーフローの時空間分解観測, 10 Mar. 2016, 10 Mar. 2016, 10 Mar. 2016
  • Masato Kondoh; Misao Mizuno; Yasuhisa Mizutani, 9th Asian Conference on Ultrafast Phenomena, Vibrational energy flow in hemeprotein monitored by time-resolved anti-Stokes ultraviolet resonance Raman spectroscopy, 22 Feb. 2016, 22 Feb. 2016, 24 Feb. 2016

Awards

  • BCSJ Award Article, 日本化学会, Masato Kondoh;Hidekazu Moritani;Taka-aki Ishibashi, May 2020
  • Poster award, 17th International Conference on Time-Resolved Vibrational Spectroscopy (TRVS 2015), Jun. 2015
  • 優秀講演賞, 第六回分子科学討論会, Oct. 2012
  • ポスター賞, 第33回溶液化学シンポジウム, Nov. 2010
  • 最優秀学生発表賞, 2010年光化学討論会, Sep. 2010
  • 優秀講演賞, 第三回分子科学討論会, Oct. 2009
  • Best presentation award, 10th KAIST-KYOTO Chemistry Symposium (First KAIST-KYOTO-TSING HUA Chemistry Symposium), Dec. 2008
  • 優秀ポスター賞, 第一回分子科学討論会, Oct. 2007

Research Projects

  • 基盤研究(C), 01 Apr. 2023, 31 Mar. 2026, 23K04661, 全内部反射ラマン分光で観るゲル－液晶相転移に伴う脂質膜中の抗菌ペプチドの構造変化, 近藤 正人, 日本学術振興会, 科学研究費助成事業 基盤研究(C), 筑波大学, 4680000, 3600000, 1080000, kaken
    対象リソースIRI
  • Grant-in-Aid for Scientific Research (C), 01 Apr. 2022, 31 Mar. 2025, 22K05016, Vibrational spectroscopic study of supported lipid bilayers with peptides, 石橋 孝章; 近藤 正人; 野嶋 優妃, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), University of Tsukuba, 4290000, 3300000, 990000, kaken
    対象リソースIRI
  • Grant-in-Aid for Scientific Research (C), 01 Apr. 2020, 31 Mar. 2023, 20K05415, Method to measure vibrational spectra of proteins in aqueous solution for detection of intermolecular interactions using solid/liquid interface, 近藤正人, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), University of Tsukuba, 4290000, 3300000, 990000, 本研究では，固体と液体の界面を利用して，水溶液中のタンパク質の振動スペクトルを水和水のものまで含めて高感度に得る新しい方法論を確立することを目的としている．その基本的なアイデアは，界面敏感な振動分光法である振動和周波発生（VSFG）分光法を，固液界面に向きを制御した上で固定したタンパク質の系に適用することである．この用途に用いるための基板として，シリカ薄膜をコートしたCaF2基板に着目した．この基板では，シリカに試料分子をシランカップリング反応により化学的に固定できる．固液界面のVSFG測定は，水の強い赤外吸収を避けるため，赤外光を基板側から入射する内部反射配置で行う必要がある．シリカは2000 cm-1以下の赤外領域で不透明であるが，薄膜としたうえで赤外領域で透明なCaF2にコートすることで，この配置での測定も可能となる．初年度は，このシリカコートCaF2基板上での有機単分子膜について，内部反射配置でヘテロダイン検出（HD）-VSFG測定を行うための取り組みを行った． まず，ゾルゲル法によってCaF2（Φ20×t5）上にシリカ薄膜（厚み~100 nm）をコートした．この基板上の有機単分子膜を内部反射配置でHD-VSFG測定するには，試料と同じ面に，参照試料を準備する必要がある．そこでシリカコート面の一部に，スパッタ法で銀薄膜を蒸着した．試料の絶対配向の情報を含んだ量である絶対的な非線形感受率を得るには，その基準となる銀の絶対的な感受率が必要である．そこで，リン脂質DPPCの脂質膜を用いて銀の絶対的な感受率を決定した．DPPCのアルキル鎖が上を向けた脂質膜の絶対的な感受率と，下を向けた膜のものでは，その符号が反転する．このことを利用して，それぞれの膜を，外部および内部反射配置でHD-VSFG測定した結果から，シリカコートCaF2に蒸着した銀薄膜の感受率の値を決定した．, Competitive research funding, kaken
    対象リソースIRI
  • Grant-in-Aid for Scientific Research (C), 01 Apr. 2019, 31 Mar. 2022, 19K05362, Study on structures and orientations of proteins in lipid bilayers by heterodyne-detected vibrational SFG spectroscopy, Ishibashi Taka-aki, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), University of Tsukuba, 4290000, 3300000, 990000, Heterodyne-detected vibrational sum frequency generation (HD-VSFG) spectroscopy and total internal reflection Raman (TR-Raman) spectroscopy were used to study the interaction of substrate-supported lipid bilayers (SLBs) with peptides and small molecules. Cholesterol, the cell membrane staining dye AP3, gramicidin, and alamethicin were examined as molecules that interact with the membrane. In particular, the interaction between SBLs and peptides was captured for the first time by Raman spectroscopy using TR-Raman spectroscopy, as a result of the improvement of the measurement system., kaken
    対象リソースIRI
  • Grant-in-Aid for Early-Career Scientists, 01 Apr. 2018, 31 Mar. 2020, 18K14175, Principal investigator, Development of total internal reflection transient grating system to monitor ion pump dynamics of rhodopsin in a lipid bilayer, Kondoh Masato, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, University of Tsukuba, 4160000, 3200000, 960000, A method based on the transient grating (TG) technique with total internal reflection geometry for probe light (TIR-TG) has been developed to detect conformational and ion transportation dynamics of rhodopsin proteins in a lipid bilayer membrane. In this study, we constructed a TIR-TG apparatus and applied it to diffusion coefficient measurements of stilbene in a dimyristoylphosphatidylcholine (DMPC) lipid bilayer membrane at a silica/water interface. As a result, we found that the stilbene molecules in the DMPC bilayer membrane show two different diffusion coefficients. This result indicates that the DMPC membrane is an inhomogeneous environment that can be described with two different effective viscosities., Competitive research funding, url;kaken
    対象リソースIRI
  • Grant-in-Aid for Young Scientists (B), 01 Apr. 2012, 31 Mar. 2014, 24750027, Interaction dynamics of solvated electron with water investigated by terahertz time-resolved dielectric spectroscopy, KONDOH Masato, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), 独立行政法人日本原子力研究開発機構, 4030000, 3100000, 930000, Experimental and technical developments for the time-resolved terahertz (THz) spectroscopy on aqueous solutions have been made in this study. First, we performed the steady-state THz spectroscopy on aqueous ionic solutions. As a result, we successfully obtained an experimental evidence for the existence of the ion effects that weaken hydrogen bonding structure in solution, demonstrating the effectiveness of THz spectroscopy for studies on a solution sample. Second, a window-less method using a liquid sheet was developed. This method salves the problem of damaging windows due to a strong pump laser pulse in the conventional method using a solution sample cell. Here, a nozzle which can produce a liquid sheet with controllable thickness from 50 to 120 micrometer has been developed and optimized to THz spectroscopy., url;kaken
    対象リソースIRI

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