HORI Sayaka
| Faculty Division of Natural Sciences Research Group of Biological Sciences | Associate Professor |
Last Updated :2025/04/27
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Optimization of the types and timing of avoidance behaviors depending on the intensity of a noxious stimulus is essential for survival; however, processing in the central nervous system and its developmental bases are largely unknown. We had reported that Caenorhabditis elegans preferentially selects one of three different types of avoidance behaviors depending on the strength of the noxious stimulus. We screened 279 neuronal transcription factors using a combination of optogenetics and RNA interference methods and identified 19 candidates required for avoidance behaviors. Our study will provide a novel molecular basis to specify the synaptic functions for complex behavior.
Name (Japanese)
HoriName (Kana)
Sayaka
Degree
Research Areas
Research History
- Apr. 2023 - Present, Nara Women's University, 研究院自然科学系生物科学領域, Associate Professor
- Apr. 2021 - Mar. 2023, Tokyo Women's Medical University, Center for Medical and Nursing Education, Division of Basic Sciences, Senior Lecturer
- Oct. 2018 - Mar. 2021, Tokyo Women's Medical University School of Medicine, Department of Physiology, Senior Lecturer
- Jan. 2010 - Sep. 2018, Tokyo Women's Medical University, Faculty of Medicine, Faculty of Medicine, 助教
- Oct. 2007 - Dec. 2009, 海洋研究開発機構 極限環境生物圏研究センター（海洋・極限環境生物圏領域）, ポストドクトラル研究員
- Apr. 2007 - Oct. 2007, 東京大学 大学院理学系研究科 生物科学専攻 細胞生理化学研究室, 産学官連携研究員
- Mar. 2007, The University of Tokyo, Graduate School of Science, Department of Biological Sciences, 博士（理学)
- Mar. 2004, The University of Tokyo, Graduate School of Science, Department of Biological Sciences
- Mar. 2002, The University of Tokyo, Faculty of Pharmaceutical Sciences
- Apr. 1998, The University of Tokyo, College of Arts and Sciences
Education
Teaching Experience
- Jul. 2024 - Present
- Jul. 2024 - Present
- Jun. 2024 - Present
- Apr. 2024 - Present
- Apr. 2024 - Present
- Apr. 2024 - Present
- Apr. 2024 - Present
- 行動生理学セミナーⅡ, Nara Women's University, Oct. 2023 - Present
- 行動生理学演習Ⅰ, Nara Women's University, Oct. 2023 - Present
- Practical Exercises on Biological and Environmental SciencesII, Nara Women's University, Oct. 2023 - Present
- Biodiversity Laboratory 2, Nara Women's University, Oct. 2023 - Present
- Animal Morphology and Histology, Nara Women's University, Oct. 2023 - Present
- Oct. 2023 - Present
- Topics in Molecular and Cellular Biology 8, Nara Women's University, Jun. 2023 - Present
- Topics in Functional Biology of Multicellular Organisms 1, Nara Women's University, May 2023 - Present
- 行動生理学演習Ⅳ, Nara Women's University, Apr. 2023 - Present
- 行動生理学セミナーⅠ, Nara Women's University, Apr. 2023 - Present
- Biology of Homeostasis, Nara Women's University, Apr. 2023 - Present
- 行動生理学特論, Nara Women's University, Apr. 2023 - Present
- Apr. 2022 - Mar. 2023
- Apr. 2022 - Mar. 2023
- Jan. 2022 - Mar. 2023
- Apr. 2021 - Mar. 2023
- Apr. 2021 - Mar. 2023
Professional Memberships
Social Activities
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■Ⅱ．研究活動実績
Published Papers
- Refereed, Zoological Letters, Springer Science and Business Media LLC, Male Caenorhabditis elegans optimizes avoidance behavior against acute and chronic stress for successful mating with hermaphrodites, Sayaka Hori; Shohei Mitani, Abstract
The optimization of avoidance behaviors in response to stress is an instinctual life function universally present in animals. In many sexually dimorphic animals, males exhibit higher stress resistance than females, but there have been no reports of comparative studies on stress resistance in sexually dimorphic hermaphrodites capable of reproducing alone. In the present study, we aimed to utilize a reversal/turn behavioral choice to conduct a comparative analysis of optimized avoidance behavior patterns in hermaphrodite and male Caenorhabditis elegans. We found that C. elegans males showed greater resistance to physical movement under acute stress and to lifespan reduction under chronic stress than C. elegans hermaphrodites. Interestingly, males exhibited a stronger avoidance behavior pattern known as “turn” than did the hermaphrodites, even in response to mild acute stress stimuli, to which they responded as if they had been exposed to strong stimuli. Stress conditions can lead to unsuccessful mating in C. elegans, and exaggerated stress avoidance in males may have biological significance for successful mating. This sexual dimorphism in avoidance behavior optimization was attributed to neural circuits downstream of the AIB neurons, the center of turn behavior, suggesting the presence of a novel mechanism distinct from previously reported neural and molecular mechanisms of avoidance behavior optimization., 17 Apr. 2025, 11, 4, Scientific journal, 10.1186/s40851-025-00250-7 - Refereed, microPublication Biology, An atonal homolog, lin-32, regulates hypodermal morphogenesis in Caenorhabditis elegans, Sayaka Hori; Shohei Mitani, 14 Feb. 2023, 10.17912/micropub.biology.000754., Scientific journal, 10.17912/micropub.biology.000754.
- Refereed, Scientific reports, The transcription factor unc-130/FOXD3/4 contributes to the biphasic calcium response required to optimize avoidance behavior., Sayaka Hori; Shohei Mitani, The central neural network optimizes avoidance behavior depending on the nociceptive stimulation intensity and is essential for survival. How the property of hub neurons that enables the selection of behaviors is genetically defined is not well understood. We show that the transcription factor unc-130, a human FOXD3/4 ortholog, is required to optimize avoidance behavior depending on stimulus strength in Caenorhabditis elegans. unc-130 is necessary for both ON responses (calcium decreases) and OFF responses (calcium increases) in AIBs, central neurons of avoidance optimization. Ablation of predicted upstream inhibitory neurons reduces the frequency of turn behavior, suggesting that optimization needs both calcium responses. At the molecular level, unc-130 upregulates the expression of at least three genes: nca-2, a homolog of the vertebrate cation leak channel NALCN; glr-1, an AMPA-type glutamate receptor; and eat-4, a hypothetical L-glutamate transmembrane transporter in the central neurons of optimization. unc-130 shows more limited regulation in optimizing behavior than an atonal homolog lin-32, and unc-130 and lin-32 appear to act in parallel molecular pathways. Our findings suggest that unc-130 is required for the establishment of some AIB identities to optimize avoidance behavior., 03 Feb. 2022, 12, 1, 1907, 1907, Scientific journal, True, 10.1038/s41598-022-05942-0
- Not Refereed, Feb. 2022, Research institution
- Refereed, PLoS Genetics, OFF-responses of interneurons optimize avoidance behaviors depending on stimulus strength via electrical synapses, Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, Jun. 2018, 14, 6, e1007477, Scientific journal, 10.1371/journal.pgen.1007477
- Refereed, Cell Reports, Elsevier B.V., An Aneuploidy-Free and Structurally Defined Balancer Chromosome Toolkit for Caenorhabditis elegans, Katsufumi Dejima; Sayaka Hori; Satoru Iwata; Yuji Suehiro; Sawako Yoshina; Tomoko Motohashi; Shohei Mitani, Balancer chromosomes are critical tools for genetic research. In C. elegans, reciprocal translocations that lead to aneuploidy have been widely used to maintain lethal and sterile mutations in stable stocks. Here, we generated a set of aneuploidy-free and structurally defined crossover suppressors that contain two overlapping inversions using the CRISPR/Cas9 system. The toolkit includes 13 crossover suppressors and covers approximately 63% of all C. elegans coding genes. Together with the classical intrachromosomal crossover suppressors, the system now covers 89% of the coding genes. We also labeled the created balancers with fluorescent and phenotypic markers. We show that the crossover suppressors are better for embryonic analysis compared with translocational balancers. Additionally, we demonstrate an efficient method to generate lethal alleles by targeting essential genes on a chromosome balanced with a crossover suppressor. The toolkit will allow more efficient experiments in which lethal and sterile mutants can be analyzed. Balancer chromosomes are critical tools for genetic research. Using the CRISPR/Cas9 system, Dejima et al. established a collection of balancer chromosomes in C. elegans. The toolkit will be useful not only for maintenance of lethal/sterile mutants but also for several other applications through customization to suit a particular use., 02 Jan. 2018, 22, 1, 232, 241, Scientific journal, 10.1016/j.celrep.2017.12.024
- Refereed, Micropublication: biology, Novel deletion alleles of a C. elegans gene Y48E1C.1, named as tm5468, tm5625 and tm5626, Hori, S; Suehiro, Y; Yoshina, S; Mitani S, Oct. 2017, https://doi.org/10.17912, /W2CQ1, Scientific journal
- Refereed, Jun. 2017, 79, 3, 44, 48
- Not Refereed, Feb. 2017, Research institution
- Refereed, Micropublication: biology, Novel deletion alleles of a C. elegans gene Y73E7A.1, named as tm6429 and tm6475. Micropublication, Suehiro, Y; Yoshina, S; Hori, S; Mitani, S, 2017, https://doi.org/10.17912/W2HH3, Scientific journal
- Refereed, Micropublication: biology, Novel deletion alleles of a C. elegans gene C38D4.9, named as tm4476 and tm4561., Yoshina S; Hori, S; Suehiro, Y; Mitani, S, 2017, https://doi.org/10.17912/W2808, Scientific journal
- Refereed, SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9, Satoru Iwata; Sawako Yoshina; Yuji Suehiro; Sayaka Hori; Shohei Mitani, Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes., Sep. 2016, 6, 33840, Scientific journal, 10.1038/srep33840
- Refereed, ELIFE, ELIFE SCIENCES PUBLICATIONS LTD, The DEG/ENaC Cation Channel Protein UNC-8 Drives Activity-Dependent Synapse Removal in Remodeling GABAergic Neurons, Tyne W. Miller-Fleming; Sarah C. Petersen; Laura Manning; Cristina Matthewman; Megan Gornet; Allison Beers; Sayaka Hori; Shohei Mitani; Laura Bianchi; Janet Richmond; David M. Miller, Genetic programming and neural activity drive synaptic remodeling in developing neural circuits, but the molecular components that link these pathways are poorly understood. Here we show that the C. elegans Degenerin/Epithelial Sodium Channel (DEG/ENaC) protein, UNC-8, is transcriptionally controlled to function as a trigger in an activity-dependent mechanism that removes synapses in remodeling GABAergic neurons. UNC-8 cation channel activity promotes disassembly of presynaptic domains in DD type GABA neurons, but not in VD class GABA neurons where unc-8 expression is blocked by the COUP/TF transcription factor, UNC-55. We propose that the depolarizing effect of UNC-8-dependent sodium import elevates intracellular calcium in a positive feedback loop involving the voltage-gated calcium channel UNC-2 and the calcium-activated phosphatase TAX-6/calcineurin to initiate a caspase-dependent mechanism that disassembles the presynaptic apparatus. Thus, UNC-8 serves as a link between genetic and activity-dependent pathways that function together to promote the elimination of GABA synapses in remodeling neurons., Jul. 2016, 5, e14599, e14599, Scientific journal, 10.7554/eLife.14599
- Not Refereed, Feb. 2016, Research institution
- Refereed, PLOS ONE, PUBLIC LIBRARY SCIENCE, A Conditional Knockout Toolkit for Caenorhabditis elegans Based on the Cre/loxP Recombination, Eriko Kage-Nakadai; Rieko Imae; Yuji Suehiro; Sawako Yoshina; Sayaka Hori; Shohei Mitani, Conditional knockout (cKO) based on site-specific recombination (SSR) technology is a powerful approach for estimating gene functions in a spatially and temporally specific manner in many model animals. In Caenorhabditis elegans (C. elegans), spatial- and temporal-specific gene functions have been largely determined by mosaic analyses, rescue experiments and feeding RNAi methods. To develop a systematic and stable cKO system in C. elegans, we generated Cre recombinase expression vectors that are driven by various tissue-specific or heat-shock promoters. Validation using Cre-mediated fluorescence protein inactivation or activation systems demonstrated successful Cre-dependent loxP excision. We established a collection of multi-copy Cre transgenic strains for each evaluated vector. To evaluate our Cre/loxP-based cKO system, we generated sid-1 deletion mutants harboring floxed sid-1 single-copy integration (SCI) using ultraviolet trimethylpsoralen (UV/TMP) methods. sid-1 mutants that were rescued by the floxed sid-1 SCI were then crossed with the Pdpy-7:: Cre strain for cKO in the hypodermis. The sid-1 cKO animals were resistant to bli-3 RNAi, which causes the Bli-phenotyple in the hypodermis, but they were sensitive to unc-22 RNAi, which leads to twitching of the body wall muscle. Our system, which is based on the combination of a transgenic Cre collection, pre-existing deletion mutants, and UV/TMP SCI methods, provided a systematic approach for cKO in C. elegans., Dec. 2014, 9, 12, e114680, Scientific journal, 10.1371/journal.pone.0114680
- Refereed, Nov. 2014, 34, 18, 19
- Refereed, FRONTIERS IN MICROBIOLOGY, FRONTIERS RESEARCH FOUNDATION, High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes, Mikihiko Kawai; Taiki Futagami; Atsushi Toyoda; Yoshihiro Takaki; Shinro Nishi; Sayaka Hori; Wataru Arai; Taishi Tsubouchi; Yuki Morono; Ikuo Uchiyama; Takehiko Ito; Asao Fujiyama; Fumio Inagaki; Hideto Takami, Marine subsurface sediments on the Pacific margin harbor diverse microbial communities even at depths of several hundreds meters below the seafloor (mbsf) or more. Previous PCR-based molecular analysis showed the presence of diverse reductive dehalogenase gene (rdhA) homologs in marine subsurface sediment, suggesting that anaerobic respiration of organohalides is one of the possible energy-yielding pathways in the organic-rich sedimentary habitat. However, primer-independent molecular characterization of rdhA has remained to be demonstrated. Here, we studied the diversity and frequency of rdhA homologs by metagenomic analysis of five different depth horizons (0.8, 5.1, 18.6, 48.5, and 107.0 mbsf) at Site C9001 off the Shimokita Peninsula of Japan. From all metagenomic pools, remarkably diverse rdhA-homologous sequences, some of which are affiliated with novel clusters, were observed with high frequency. As a comparison, we also examined frequency of dissimilatory sulfite reductase genes (dsrAB), key functional genes for microbial sulfate reduction. The dsrAB were also widely observed in the metagenomic pools whereas the frequency of dsrAB genes was generally smaller than that of rdhA-homologous genes. The phylogenetic composition of rdhA-homologous genes was similar among the five depth horizons. Our metagenomic data revealed that subseafloor rdhA homologs are more diverse than previously identified from PCR-based molecular studies. Spatial distribution of similar rdhA homologs across wide depositional ages indicates that the heterotrophic metabolic processes mediated by the genes can be ecologically important functioning in the organic-rich subseafloor sedimentary biosphere., Mar. 2014, 5, 80, 1, 15, Scientific journal, 10.3389/fmicb.2014.00080
- Nov. 2013, 33, 22, 23
- Refereed, PloS one, 11, Correction: Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis., Kaneko K; Ikeda T; Nagai M; Hori S; Umatani C; Tadano H; Ugajin A; Nakaoka T; Paul RK; Fujiyuki T; Shirai K; Kunieda T; Takeuchi H; Kubo T, Nov. 2013, 8, 10.1371/annotation/1fa31a02-1b58-4361-98eb-5c213e5d5336
- Refereed, PLOS ONE, PUBLIC LIBRARY SCIENCE, Novel Middle-Type Kenyon Cells in the Honeybee Brain Revealed by Area-Preferential Gene Expression Analysis, Kumi Kaneko; Tsubomi Ikeda; Mirai Nagai; Sayaka Hori; Chie Umatani; Hiroto Tadano; Atsushi Ugajin; Takayoshi Nakaoka; Rajib Kumar Paul; Tomoko Fujiyuki; Kenichi Shirai; Takekazu Kunieda; Hideaki Takeuchi; Takeo Kubo, The mushroom bodies (a higher center) of the honeybee (Apis mellifera L) brain were considered to comprise three types of intrinsic neurons, including large- and small-type Kenyon cells that have distinct gene expression profiles. Although previous neural activity mapping using the immediate early gene kakusei suggested that small-type Kenyon cells are mainly active in forager brains, the precise Kenyon cell types that are active in the forager brain remain to be elucidated. We searched for novel gene(s) that are expressed in an area-preferential manner in the honeybee brain. By identifying and analyzing expression of a gene that we termed mKast (middle-type Kenyon cell-preferential arrestin-related protein), we discovered novel 'middle-type Kenyon cells' that are sandwiched between large- and small-type Kenyon cells and have a gene expression profile almost complementary to those of large- and small-type Kenyon cells. Expression analysis of kakusei revealed that both small-type Kenyon cells and some middle-type Kenyon cells are active in the forager brains, suggesting their possible involvement in information processing during the foraging flight. mKast expression began after the differentiation of small- and large-type Kenyon cells during metamorphosis, suggesting that middle-type Kenyon cells differentiate by modifying some characteristics of large- and/or small-type Kenyon cells. Interestingly, CaMKII and mKast, marker genes for large- and middle-type Kenyon cells, respectively, were preferentially expressed in a distinct set of optic lobe (a visual center) neurons. Our findings suggested that it is not simply the Kenyon cell-preferential gene expression profiles, rather, a 'clustering' of neurons with similar gene expression profiles as particular Kenyon cell types that characterize the honeybee mushroom body structure., Aug. 2013, 8, 8, e71732, Scientific journal, 10.1371/journal.pone.0071732
- Refereed, NEURON, CELL PRESS, Sensory Neuron Fates Are Distinguished by a Transcriptional Switch that Regulates Dendrite Branch Stabilization, Cody J. Smith; Timothy O'Brien; Marios Chatzigeorgiou; W. Clay Spencer; Elana Feingold-Link; Steven J. Husson; Sayaka Hori; Shohei Mitani; Alexander Gottschalk; William R. Schafer; David M. Miller, Sensory neurons adopt distinct morphologies and functional modalities to mediate responses to specific stimuli. Transcription factors and their downstream effectors orchestrate this outcome but are incompletely defined. Here, we show that different classes of mechanosensory neurons in C. elegans are distinguished by the combined action of the transcription factors MEC-3, AHR-1, and ZAG-1. Low levels of MEC-3 specify the elaborate branching pattern of PVD nociceptors, whereas high MEC-3 is correlated with the simple morphology of AVM and PVM touch neurons. AHR-1 specifies AVM touch neuron fate by elevating MEC-3 while simultaneously blocking expression of nociceptive genes such as the MEC-3 target, the claudin-like membrane protein HPO-30, that promotes the complex dendritic branching pattern of PVD. ZAG-1 exercises a parallel role to prevent PVM from adopting the PVD fate. The conserved dendritic branching function of the Drosophila AHR-1 homolog, Spineless, argues for similar pathways in mammals., Jul. 2013, 79, 2, 266, 280, Scientific journal, 10.1016/j.neuron.2013.05.009
- Refereed, Bioscience, Biotechnology and Biochemistry, Active bacterial flora surrounding foraminifera (xenophyophorea) living on the deep-sea floor, Sayaka Hori; Masashi Tsuchiya; Shinro Nishi; Wataru Arai; Takao Yoshida; Hideto Takami, Bacteria form unique ecosystems by coexisting with large organisms. Here we present the first evidence of active flora surrounding xenophyophorea revealed through clone analyses of environmental ribosomal RNA gene sequences. The flora included eight phyla in the xenophyophorean cells with agglutinated test. The major operational taxonomic units were unique from that in the near-surface sediment. This flora appears to be formed by coexistence with xenophyophores., 2013, 77, 2, 381, 384, Scientific journal, 10.1271/bbb.120663
- Refereed, G3-GENES GENOMES GENETICS, GENETICS SOCIETY AMERICA, Large-Scale Screening for Targeted Knockouts in the Caenorhabditis elegans Genome, Robert Barstead; Gary Moulder; Beth Cobb; Stephen Frazee; Diane Henthorn; Jeff Holmes; Daniela Jerebie; Martin Landsdale; Jamie Osborn; Cherilyn Pritchett; James Robertson; John Rummage; Ed Stokes; Malani Vishwanathan; Shohei Mitani; Keiko Gengyo-Ando; Osamu Funatsu; Sayaka Hori; Rieko Imae; Eriko Kage-Nakadai; Hiroyuki Kobuna; Etsuko Machiyama; Tomoko Motohashi; Muneyoshi Otori; Yuji Suehiro; Sawako Yoshina; Donald Moerman; Mark Edgley; Ryan Adair; B. J. Allan; Vinci Au; Iasha Chaudhry; Rene Cheung; Owen Dadivas; Simon Eng; Lisa Fernando; Angela Fisher; Stephane Flibotte; Erin Gilchrist; Allison Hay; Peter Huang; Rebecca Worsley Hunt; Christine Kwitkowski; Joanne Lau; Norris Lee; Lucy Liu; Adam Lorch; Candy Luck; Jason Maydan; Sheldon McKay; Angela Miller; Greg Mullen; Candice Navaroli; Sarah Neil; Rebecca Hunt-Newbury; Mikhaela Partridge; Jaryn Perkins; Anna Rankin; Greta Raymant; Nadereh Rezania; Alexandra Rogula; Bin Shen; Greg Stegeman; Angela Tardif; Jon Taylor; Mariana Veiga; Tina Wang; Rick Zapf, The nematode Caenorhabditis elegans is a powerful model system to study contemporary biological problems. This system would be even more useful if we had mutations in all the genes of this multicellular metazoan. The combined efforts of the C. elegans Deletion Mutant Consortium and individuals within the worm community are moving us ever closer to this goal. At present, of the 20,377 protein-coding genes in this organism, 6764 genes with associated molecular lesions are either deletions or null mutations (WormBase WS220). Our three laboratories have contributed the majority of mutated genes, 6841 mutations in 6013 genes. The principal method we used to detect deletion mutations in the nematode utilizes polymerase chain reaction (PCR). More recently, we have used array comparative genome hybridization (aCGH) to detect deletions across the entire coding part of the genome and massively parallel short-read sequencing to identify nonsense, splicing, and missense defects in open reading frames. As deletion strains can be frozen and then thawed when needed, these strains will be an enduring community resource. Our combined molecular screening strategies have improved the overall throughput of our gene-knockout facilities and have broadened the types of mutations that we and others can identify. These multiple strategies should enable us to eventually identify a mutation in every gene in this multicellular organism. This knowledge will usher in a new age of metazoan genetics in which the contribution to any biological process can be assessed for all genes., Nov. 2012, 2, 11, 1415, 1425, Scientific journal, 10.1534/g3.112.003830
- Aug. 2012, 32, 22, 23
- Refereed, BMC BIOTECHNOLOGY, BIOMED CENTRAL LTD, Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method, Eriko Kage-Nakadai; Hiroyuki Kobuna; Osamu Funatsu; Muneyoshi Otori; Keiko Gengyo-Ando; Sawako Yoshina; Sayaka Hori; Shohei Mitani, Background: Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silenced in the germline. Mos1 transposon or microparticle bombardment methods have been developed to create single-or low-copy chromosomal integrated lines. Here we report an alternative method using ultraviolet trimethylpsoralen (UV/TMP) to generate single/low-copy gene integrations.
Results: We successfully integrated low-copy transgenes from extrachromosomal arrays using positive selection based on temperature sensitivity with a vps-45 rescue fragment and negative selection based on benzimidazole sensitivity with a ben-1 rescue fragment. We confirmed that the integrants express transgenes in the germline. Quantitative PCR revealed that strains generated by this method contain single-or low-copy transgenes. Moreover, positive selection marker genes flanked by LoxP sites were excised by Cre recombinase mRNA microinjection, demonstrating Cre-mediated chromosomal excision for the first time in C. elegans.
Conclusion: Our UV/TMP integration method, based on familiar extrachromosomal transgenics, provides a useful approach for generating single/low-copy gene integrations., Jan. 2012, 12, 1, 1, Scientific journal, 10.1186/1472-6750-12-1 - Refereed, APIDOLOGIE, SPRINGER FRANCE, Expression of two microRNAs, ame-mir-276 and-1000, in the adult honeybee (Apis mellifera) brain, Sayaka Hori; Kumi Kaneko; Takeshi H. Saito; Hideaki Takeuchi; Takeo Kubo, To identify candidate microRNAs involved in post-transcriptional regulation of brain (region)selective gene expression in the adult honeybee brain, we isolated eight microRNAs: seven known microRNAs, ame-mir-2-1, -8, 13a, -34, -276, -317, -1000, and one novel one, named mir-hbd, that has significant sequence similarity with the Drosophila dme-mir-11. Among them, ame-mir-1000 and -276 were expressed in a brain-selective and -preferential manner, respectively, in workers and drones. In particular, ame-mir-276-expression was enriched in the optic lobes and in the small type-Kenyon cells of the mushroom bodies in the nurse bee, forager, queen, and drone brains. Almost all predicted targets of ame-mir-1000 and -276 encode neural function related genes, suggesting the involvement in neural function of both microRNAs., Jan. 2011, 42, 1, 89, 102, Scientific journal, 10.1051/apido/2010032
- Refereed, PLOS ONE, PUBLIC LIBRARY SCIENCE, In Situ Hybridization Analysis of the Expression of Futsch, Tau, and MESK2 Homologues in the Brain of the European Honeybee (Apis mellifera L.), Kumi Kaneko; Sayaka Hori; Mai M. Morimoto; Takayoshi Nakaoka; Rajib Kumar Paul; Tomoko Fujiyuki; Kenichi Shirai; Akiko Wakamoto; Satomi Tsuboko; Hideaki Takeuchi; Takeo Kubo, Background: The importance of visual sense in Hymenopteran social behavior is suggested by the existence of a Hymenopteran insect-specific neural circuit related to visual processing and the fact that worker honeybee brain changes morphologically according to its foraging experience. To analyze molecular and neural bases that underlie the visual abilities of the honeybees, we used a cDNA microarray to search for gene(s) expressed in a neural cell-type preferential manner in a visual center of the honeybee brain, the optic lobes (OLs).
Methodology/Principal Findings: Expression analysis of candidate genes using in situ hybridization revealed two genes expressed in a neural cell-type preferential manner in the OLs. One is a homologue of Drosophila futsch, which encodes a microtubule-associated protein and is preferentially expressed in the monopolar cells in the lamina of the OLs. The gene for another microtubule-associated protein, tau, which functionally overlaps with futsch, was also preferentially expressed in the monopolar cells, strongly suggesting the functional importance of these two microtubule-associated proteins in monopolar cells. The other gene encoded a homologue of Misexpression Suppressor of Dominant-negative Kinase Suppressor of Ras 2 (MESK2), which might activate Ras/MAPK-signaling in Drosophila. MESK2 was expressed preferentially in a subclass of neurons located in the ventral region between the lamina and medulla neuropil in the OLs, suggesting that this subclass is a novel OL neuron type characterized by MESK2-expression. These three genes exhibited similar expression patterns in the worker, drone, and queen brains, suggesting that they function similarly irrespective of the honeybee sex or caste.
Conclusions: Here we identified genes that are expressed in a monopolar cell (Amfutsch and Amtau) or ventral medulla-preferential manner (AmMESK2) in insect OLs. These genes may aid in visualizing neurites of monopolar cells and ventral medulla cells, as well as in analyzing the function of these neurons., Feb. 2010, 5, 2, e9213, Scientific journal, 10.1371/journal.pone.0009213 - Refereed, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 11, [New approach toward understanding of neural basis of the honeybee "dance communication"], Kiya T; Hori S; Takeuchi H; Kubo T, Sep. 2008, 53, 11, 1368, 1374
- Refereed, JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, SPRINGER, Associative learning and discrimination of motion cues in the harnessed honeybee Apis mellifera L., Sayaka Hori; Hideaki Takeuchi; Takeo Kubo, We previously studied a conditioning paradigm to associate the proboscis extension reflex (PER) with monochromatic light (conditioned stimulus; CS) in harnessed honeybees. Here, we established a novel conditioning paradigm to associate the PER with a motion cue generated using graphics interchange format (GIF) animations with a speed of 12 mm/s speed and a frame rate of 25 Hz as the CS, which were projected onto a screen consisting of a translucent circular cone that largely covered the visual field of the harnessed bee using two liquid crystal projectors. The acquisition rate reached a plateau at approximately 40% after seven trials, indicating that the bees were successfully conditioned with the motion cue. We demonstrated four properties of the conditioning paradigm. First, the acquisition rate was enhanced by antennae deprivation, suggesting that sensory input from the antennae interferes with the visual associative learning. Second, bees conditioned with a backward-direction motion cue did not respond to the forward-direction, suggesting that bees can discriminate the two directions in this paradigm. Third, the bees can retain memory for motion cue direction for 48 h. Finally, the acquisition rate did not differ significantly between foragers and nurse bees., Aug. 2007, 193, 8, 825, 833, Scientific journal, 10.1007/s00359-007-0234-x
- Refereed, Establishment of visual associative learning paradigm using harnessed honeybee and analysis of its visual cognitive capacities, Sayaka Hori, 22 Mar. 2007, Doctoral thesis
- Refereed, Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology, 7, Associative visual learning, color discrimination, and chromatic adaptation in the harnessed honeybee Apis mellifera L., Sayaka Hori; Hideaki Takeuchi; Kentaro Arikawa; Michiyo Kinoshita; Naoko Ichikawa; Masami Sasaki; Takeo Kubo, We studied associative visual learning in harnessed honeybees trained with monochromatic lights associated with a reward of sucrose solution delivered to the antennae and proboscis, to elicit the proboscis extension reflex (PER). We demonstrated five properties of visual learning under these conditions. First, antennae deprivation significantly increased visual acquisition, suggesting that sensory input from the antennae interferes with visual learning. Second, covering the compound eyes with silver paste significantly decreased visual acquisition, while covering the ocelli did not. Third, there was no significant difference in the visual acquisition between nurse bees, guard bees, and foragers. Fourth, bees conditioned with a 540-nm light stimulus exhibited light-induced PER with a 618-nm, but not with a 439-nm light stimulus. Finally, bees conditioned with a 540-nm light stimulus exhibited PER immediately after the 439-nm light was turned off, suggesting that the bees reacted to an afterimage induced by prior adaptation to the 439-nm light that might be similar to the 540-nm light. © Springer-Verlag 2006., Jul. 2006, 192, 7, 691, 700, Scientific journal, 10.1007/s00359-005-0091-4
- Refereed, GENES TO CELLS, BLACKWELL PUBLISHING LTD, GRIP1 tau, a novel PDZ domain-containing transcriptional activator, cooperates with the testis-specific transcription elongation factor SII-T1, A Nakata; T Ito; M Nagata; S Hori; K Sekimizu, SII-T1 is a tissue-specific member of the transcription elongation factor S-II that is expressed specifically in male germ cells. In the present study, we have identified a protein named GRIP1tau interacting with SII-T1 by yeast two-hybrid screening. GRIP1tau is a novel isoform of glutamate receptor-interacting protein 1 (GRIP1) that associates with the cytoplasmic domain of the alpha-amino-3-hydroxy-5-methyl-4-isoaxazolepropionate (AMPA)-type glutamate receptor. GRIP1tau is a testis-specific nuclear protein that activates transcription when fused with a GAL4 DNA binding domain in GAL4-responsive reporter gene assays. The transactivation domain of GRIP1tau overlapped with the region essential for interaction with SII-T1, as revealed by co-immunoprecipitation assays. Also, transactivation by GRIP1tau was stimulated by SII-T1 in a dose-dependent manner. Therefore, we propose that GRIP1tau is a novel testis-specific transcriptional activator regulated by interaction with the testis-specific transcription elongation factor SII-T1., Nov. 2004, 9, 11, 1125, 1135, Scientific journal, 10.1111/j.1365-2443.2004.00795.x
MISC
- Not Refereed, 05 Sep. 2020
- Not Refereed, 日本分子生物学会年会プログラム・要旨集(Web), atonal,math1ホモログの転写因子lin‐32は線虫C.elegansの逃避行動における介在神経の分化に必要, HORI SAYAKA; ODA SHIGEKAZU; SUEHIRO YUJI; IINO YUICHI; MITANI SHOHEI, 2013, 36th, 2P-0699 (WEB ONLY)
- Not Refereed, 日本分子生物学会年会プログラム・要旨集(Web), 光遺伝学技術を利用した,侵害刺激からの逃避行動を規定する神経回路形成・遺伝子発現制御におけるbHLH型転写因子lin‐32の新機能の解析, HORI SAYAKA; ODA SHIGEKAZU; SUEHIRO YUJI; IINO YUICHI; MITANI SHOHEI, 2012, 35th, 1W11III-1 (WEB ONLY)
- Not Refereed, NEUROSCIENCE RESEARCH, ELSEVIER IRELAND LTD, Novel screening of molecules deciding synaptic connections using behavior induced by Channelrhodopsin-2 in C. elegans, Sayaka Hori; Shohei Mitani, 2011, 71, E132, E133, Summary international conference, 10.1016/j.neures.2011.07.569
- Not Refereed, Analysis of the brain substructures using region-preferential genes of the honeybee, Dec. 2009, Summary national conference
- Not Refereed, 第32回日本分子生物学会要旨集, Inactivation of alfa-proteobacteria and activation of gamma-proteobacteria induced by a new candidate genus Xenophyophores (giant foraminifera) at a depth 7111m in the Izu-Ogasawara Trench, Dec. 2009, Summary national conference
- Not Refereed, 2009, 25, 35, 35
- Not Refereed, Protein, nucleic acid and enzyme, New approach toward understanding of neural basis of the honeybee "dance communication", Sep. 2008, 53, 11, 1368, 1374
- Not Refereed, FEBS JOURNAL, BLACKWELL PUBLISHING, Identification and analysis of genes that are expressed strongly in the optic lobes in the honeybee brain, K. Kaneko; S. Hori; H. Takeuchi; R. K. Paul; T. Fujiyuki; K. Shirai; A. Wakamoto; T. Kubo, Jun. 2008, 275, 147, 147, Summary international conference
- Not Refereed, 12 Mar. 2008, 52, 129, 129
- Not Refereed, 02-040 Metagenomic analysis of deep subsurface core samples collected in an offing of the Shimokita Peninsula on the northwest Honshu, Japan(Soil ecosystem), Takami Hideto; Toyoda Atsushi; Itoh Tekehiko; Tsubouchi Taishi; Matsui Satomi; Hori Sayaka; Nishi Shinrou; Arai Wataru; Morono Yuki; Inagaki Fumio; Takai Ken, 2008, 24, 78, 78
- Not Refereed, 09-161 Bacterial population based on 16SrRNA genes of deep subsurface core sample collected in an offing of the Simokita Peninsula(Classification/Phylogenetic analysis), Nishi Shinro; Toyoda Atsushi; Matsui Satomi; Tsubouchi Taishi; Hori Sayaka; Arai Wataru; Morono Yuki; Inagaki Fumio; Takai Ken; Takami Hideto, 2008, 24, 139, 139
- Not Refereed, The 8th Japanese Drosophila Research Conference, Associative learning and discrimination of visual motion cues in the harnessed honeybees (Apis mellifera L), Hori, S; Takeuchi, H; Kubo, T, Jul. 2007, Summary international conference, 10.1007/s00359-007-0234-x
- Not Refereed, ZOOLOGICAL SCIENCE, ZOOLOGICAL SOC JAPAN, Identification and analysis of genes that are expressed strongly in the optic lobes in the honeybee brain, Kumi Kaneko; Sayaka Hori; Hideaki Takeuchi; Rajib Paul; Tomoko Fujiyuki; Kenichi Shirai; Akiko Wakamoto; Takeo Kubo, Dec. 2006, 23, 12, 1191, 1191, Summary international conference
- Not Refereed, 01 Mar. 2005, 49, 64, 64
- Not Refereed, ZOOLOGICAL SCIENCE, ZOOLOGICAL SOC JAPAN, Analysis of molecular basis of associative visual learning in the honeybee Apis mellifera L., Sayaka Hori; Hideaki Takeuchi; Kentaro Arikawa; Michiyo Kinoshita; Masami Sasaki; Naoko Ichikawa; Takeo Kubo, Dec. 2004, 21, 12, 1315, 1316, Summary international conference
- Not Refereed, 01 Mar. 2004, 48, 114, 114
- Not Refereed, 01 Mar. 2003, 47, 93, 93
- Zoological science, Zoological Society of Japan, ASSOCIATIVE VISUAL LEARNING IN HONEYBEES APIS MELLIFERA L.(Physiology,Abstracts of papers presented at the 74^
Annual Meeting of the Zoological Society of Japan) :, Hori Sayaka; Takeuchi Hideaki; Sasaki Masami; Ichikawa Naoko; Kubo Takeo, 2003, 20, 12, 1587, 1587 Books etc
Presentations
- Public discourse, 15 Mar. 2025
- 20 Dec. 2024, 02 Dec. 2024 - 20 Dec. 2024
- nFuture 2024, Establishment of a Simple Behavior Analysis Method Using a Paintbrush, 27 Aug. 2024, 27 Aug. 2024 - 28 Aug. 2024
- Sayaka Hori, Future of the Nematodes Studies 2023, A prototype circuit model for sex differences in optimization of avoidance behavior, 18 Aug. 2023, 17 Aug. 2023 - 18 Aug. 2023
- 03 Sep. 2021, 02 Sep. 2021 - 04 Sep. 2021
- Sayaka Hori; Shohei Mitani, 23rd International C. elegans Conference（Online）, Contribution of a FOXD3/4 ortholog to optimization of avoidance behavior mediated by pre- and postsynaptic gene expression for a biphasic calcium response, 23 Jun. 2021, 21 Jun. 2021 - 24 Jun. 2021
- 05 Sep. 2020, 04 Sep. 2020 - 05 Sep. 2020
- 03 Dec. 2019
- Sayaka Hori, Neuro2019, Forkhead box transcription factors determine pre-synaptic specificity and synaptic transmission efficiency of a strength-dependent sensory processing circuit, 25 Jul. 2019, False
- 24 Jul. 2019
- 01 Jun. 2019, False
- Sayaka Hori; Shohei Mitani, 次世代脳冬のシンポジウム, Forkhead box transcription factors determine synaptic specificity of a strength-dependent sensory processing circuit, 13 Dec. 2018, False
- 06 Dec. 2018
- 28 Nov. 2018
- Sayaka Hori; Shohei Mitani, Forkhead box D4 transcription factor homolog, unc-130, affects sensory processing depending on stimulus strength, 27 Jul. 2018, False
- 26 Jul. 2018
- 20 Jul. 2017
- Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, Neuroscience2017, A proneural Atonal defines sensory processing depending on stimulus strength via regulating electrical synapses, 20 Jul. 2017, False
- Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, 21th International C.elegans Conference, OFF-responding Interneurons Control Stimulus Strength-Dependent Sensory Processing, 23 Jun. 2017, True
- Katsufumi Dejima; Sayaka Hori; Satoru Iwata; Sawako Yoshina; Yuji Suehiro; Tomoko Motohashi; Shohei Mitani, 21th International C.elegans Conference, Balancer chromosome toolkit for C. elegans., 21 Jun. 2017
- 30 Nov. 2016
- Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, CeNeuro2016,, Transcription factor lin-32 is essential for synaptogenesis in AIB interneuron, sustained neck muscle contraction and aversive behavior,, 29 Jul. 2016, True
- Iwata, S; Dejima, K; Hori, S; Yoshina, S; Suehiro, Y; Mitani S, 7th Asia-Pacific C. elegans meeting, Genetic engineering of chromosomal balancer toolkit for gene-clusters mediated by CRISPR/Cas9 in Caenorhabditis elegans, 25 Jun. 2016
- Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, Analysis of novel functions of an atonal homolog in aversive behavior and gap junction formation, 23 Mar. 2016, False
- 01 Dec. 2015
- Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, Analysis of neural mechanisms in behavioral switch in response to stimulating strength, 28 Jul. 2015, False
- Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, C. elegans 2015 20th International Meeting, Transcription factor lin-32 is essential for synaptogenesis in AIB interneuron, sustained neck muscle contraction and aversive behavior, 28 Jun. 2015, True
- 12 Dec. 2014, False
- 25 Nov. 2014
- 15 May 2014
- Dec. 2013, False
- 31 Aug. 2013, False
- Tyne W Miller; Sarah C Petersen; Megan E Gornet; Ying Wang; Han Lu; Cristina Matthewman; Laura Bianchi; Janet E Richmond; Shohei Mitani; Sayaka Hori; David M Mille, 19th International C. elegans Meeting, The degenerin family ion channel UNC-8 promotes activity-dependent remodeling of GABAergic synapses in C. elegans., 28 Jun. 2013, True
- S. Hori; S. Oda; Y. Suehiro; Y. Iino; S. Mitani, 19th International C. elegans Meeting, Combination of optogenetics and reverse genetics: novel behavior screening for regulators of neural differentiation, Jun. 2013
- 15 May 2013
- 11 Dec. 2012
- Sayaka Hori; Shigekazu Oda; Yuji Suehiro; Yuichi Iino; Shohei Mitani, Novel function of bHLH transcriptional factor lin-32 in avoidance cir cuit in C. elegans., Dec. 2012
- Sayaka Hori; Shohei Mitani, Neuroscience 2012, Novel function of bHLH transcriptional factor lin-32 in escape/avoidance circuit in C. elegans, Sep. 2012
- 13 Dec. 2011, 13 Dec. 2011 - 16 Dec. 2011, False
- Dec. 2011, False
- Sayaka Hori; Shohei Mitani, Novel screening of molecules deciding synaptic connections using behavior induced by Channelrhodopsin-2 in C. elegans, Sep. 2011, False
- Aug. 2011, False
- 2010, False
- 21 Nov. 2009
- Sayaka Hori; Tsuchiya Masashi; Shinro Nishi; Wataru Arai; Takao Yoshida; Hideto Takami, International Symposium on Chemosynthesis-Based Ecosystems (4th CBE), Bacterial diversity surrounding a new candidate genes Xenophyophores (Foraminifera) discovered at a depth 7111m near the Boso Peninsula, 04 Jul. 2009, 29 Jun. 2009 - 03 Jul. 2009
- Hori, S; Takeuchi, H; Kubo, T, Eighth International Congress of Neuroethology, Associative learning and discrimination of visual motion cues in harnessed honeybees, 2007, 22 Jul. 2007 - 27 Jul. 2007
- 05 Jul. 2007, 05 Jul. 2007 - 06 Jul. 2007
- Hori, S; Takeuchi, H; Kubo, T, SECOND EUROPEAN CONFERENCE OF APIDOLOGY, Associative visual learning, color discrimination, and chromatic adaptation in the harnessed honeybee, 11 Sep. 2006, 10 Sep. 2006 - 14 Sep. 2006
- Sayaka Hori, Würzburg Biozentrum (Closed meeting), Würzburg University, Würzburg (Germany), Visual associative learning in the harnessed honeybee, 08 Sep. 2006, True
- Hori, S, Closed meeting, Université Paul-Sabatier, Toulouse (France), Visual associative learning in the harnessed honeybee, Sep. 2006
- 07 Mar. 2005
- Feb. 2005
- Oral presentation
Awards
- Dec. 2024
- Sep. 2024
- Outstanding Poster Award, nFuture 2024, Aug. 2024, Establishment of a Simple Behavior Analysis Method Using a Paintbrush
- Nest Leader Award, Future of the Nematodes Studies 2023, Sayaka Hori, Aug. 2023, A prototype circuit model for sex differences in optimization of avoidance behavior, 40218121, rm:research_project_id
- Jun. 2022
- Apr. 2021
- Hori et al., 2006が重要論文として推薦されました。, Faculty of 1000, Sayaka Hori, 2006
- Mar. 2004
- 19th Kyushu High School Broadcasting Contest Kumamoto Prefecture Announcement Division Prize, NHK, Sayaka Hori, Nov. 1997
- 18th Kyushu High School Broadcasting Contest Kumamoto Prefecture Announcement Division Prize, NHK, Sayaka Hori, Nov. 1996
Research Projects
- ダイバーシティ研究環境実現イニシアティブ（女性リーダー育成型）, Feb. 2025 - Mar. 2025, HJ2R06015, Principal investigator, 雄特有の逃避行動最適化の神経・分子機序の解明と基礎モデルの構築, 堀沙耶香,末廣勇司, 奈良女子大学, 令和６年度 奈良女子大学 女性研究者 大型共同研究支援経費（追加募集）, 奈良女子大学, rm:published_papers
- Dec. 2024 - Mar. 2025, 未定, 令和６年度II期教育研究支援員制度, 国立大学法人奈良国立大学機構 奈良女子大学
- Aug. 2024 - Mar. 2025, Principal investigator, Analysis of a prototype circuit model generating sexual dimorphism in avoidance behavior optimization, Sayaka Hori, 山田科学振興財団, 2024年度研究援助 女性活躍支援枠, 国立大学法人奈良国立大学機構 奈良女子大学, rm:published_papers
- Oct. 2022 - Nov. 2024, 2200082, Principal investigator, A prototype synaptic circuit model of sex differences in optimization of avoidance behavior, 堀沙耶香; 辻野賢治, The Sumitomo Foundation, Basic Science Research Projects, 東京女子医科大学, 奈良女子大学, rm:awards
- Aug. 2024, Principal investigator, 未定, 堀沙耶香, 国立大学法人奈良国立大学機構 奈良女子大学, 令和６年度研究スキルアップ経費, 国立大学法人奈良国立大学機構 奈良女子大学
- Mar. 2024 - Mar. 2024, Coinvestigator, 遺伝子のはたらきをみてみよう, 堀沙耶香, 寄付金「関西科学塾」, rm:social_contribution
- Mar. 2024 - Mar. 2024, Coinvestigator, 遺伝子のはたらきをみてみよう, 堀 沙耶香, 地域連携事業「サイエンス発信広場」, rm:social_contribution
- Apr. 2023 - Mar. 2024, Principal investigator, 逃避行動最適化の性差と破綻の回路モデル, 国立大学法人奈良国立大学機構 奈良女子大学, 令和5年度 奈良女子大学研究推進プロジェクト経費, 国立大学法人奈良国立大学機構 奈良女子大学
- Aug. 2023, Principal investigator, 令和5年度研究スキルアップ経費, 堀 沙耶香, 国立大学法人奈良国立大学機構 奈良女子大学, 国立大学法人奈良国立大学機構 奈良女子大学
- May 2023, Principal investigator, 令和５年度II期教育研究支援員制度, 堀 沙耶香, 国立大学法人奈良国立大学機構 奈良女子大学
- Apr. 2019 - Mar. 2023, Principal investigator, 逃避行動最適化の１神経細胞における神経応答のシナプス解剖学的解析, 堀沙耶香, 文部科学省, 科学研究費補助金 基盤研究(C), 4200000, 0, 0, Competitive research funding, rm:published_papers
- Oct. 2020 - Mar. 2022, Principal investigator, Analysis of the molecular basis of the prototype circuit for optimization of avoidance behavior, Sayaka Hori, 公益財団法人 大隅基礎科学創成財団, Ohsumi Frontier Science Foundation, 東京女子医科大学, rm:published_papers
- Apr. 2019 - Mar. 2020, Principal investigator, 行動最適化の原型回路をモデルとした精神疾患リスク因子のシナプス機能の解析, 堀沙耶香, 公益信託 成茂, 成茂神経科学研究助成基金, 500000, 500000, 0, Competitive research funding, rm:published_papers
- Oct. 2018 - Mar. 2019, Principal investigator, 逃避行動様式の決定に関する分子遺伝学的研究, 堀沙耶香, 東京女子医科大学, 研究支援員制度, 1920000, 1920000, 0, Competitive research funding, rm:misc
- Sep. 2016 - Mar. 2017, 高性能な線虫バランサーの整備, 三谷昌平, AMED, ナショナルバイオリソースプロジェクト 基盤技術整備プログラム, 6000000, 6000000, 0, 研究協力者として参加。, Competitive research funding
- Apr. 2016 - Mar. 2017, Principal investigator, 逃避行動選択の鍵となる電気的シナプスの発生制御と神経回路基盤の解析, 堀沙耶香, 東京女子医科大学, 基礎研究振興費, 700400, 700400, 0, Competitive research funding
- Apr. 2013 - Mar. 2016, Principal investigator, 逃避行動を規定する神経回路発生の分子機序の解析, Sayaka Hori, The Ministry of Education,Culuture,Sports,Science and Technology (MEXT), Grant-in-Aid for Young Scientists (B), 0, 0, 0, Competitive research funding
- Apr. 2012 - Mar. 2015, 高浸透圧刺激への忌避行動を規定する転写因子群の分子・神経機能の研究, 三谷昌平, 私立大学等経常費補助金 大学間連携等による共同研究, 0, 0, 0, Competitive research funding
- Apr. 2010 - Mar. 2011, Principal investigator, 行動変化を指標にした軸索投射を規定する分子基盤の網羅的スクリーニングと機能解析, 堀沙耶香, 東京女子医科大学, 基礎プール, 0, 0, 0, Competitive research funding
- 2010 - 2011, Principal investigator, 人為的誘発行動を指標にしたシナプス選択性を規定する分子基盤のスクリーニングと機能解析, 堀 沙耶香, ナリシゲ, 成茂神経科学研究助成基金, 0, 0, 0, Competitive research funding
- Grant-in-Aid for Scientific Research (B), 2008 - 2010, 20310124, Coinvestigator not use grants, Preparation method of environmental DNA sample for conservation of the genetic resource in the deep subsurface biosphere and analysis of biodiviersity harbored in the DNA, TAKAMI Hideto; HORI Sayaka; TSUBOUCHI Taishi; TOYODA Atsushi; HORI Sayaka; NISHI Shinro, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Japan Agency for Marine-Earth Science and Technology, 0, 0, 0, We attempted to extract environmental DNA mainly from ocean drilling core samples recovered offshore Shimokita Peninsula. The extracted DNA decreased with increasing of depth but the estimated cell number from DNA amount was not so different from those counted by microscopy. Thus, It is though that the DNA extraction method was almost successfully established. We also analyzed to figure out biodiversity based on 16S rDNA and the predicted metabolic potential from the identified genes in the DNA samples. JS1 and Chloroflexi were found to be predominant taxa as described previously up to a depth of 100m. In each DNA sample from different depth, 60,000-80,000 genes were identified. The sulfur respiration-related genes and methanogenesis-related genes were highlighted in sallower and deeper horizons, respectively., Competitive research funding, url
- Grant-in-Aid for Young Scientists (Start-up), 2008 - 2009, 20810047, Principal investigator, Research of microbe in the deepest subsurface biosphere based on RNA analysis, HORI Sayaka, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Japan Agency for Marine-Earth Science and Technology, 0, 0, 0, To clarify bacterial diversity in the geological static deep-sea sediment, I tried to extract RNA to make cDNA libraries. The Automatic Bottom Inspection and Sampling Mobile "ABISMO" succeeded sampling at the deepest Mariana Trench, however, the included microbe was lower amount than previous report. Then, preliminary experiment was performed using the sediment in the static Izu-Ogasawara Trench. The population of Chloroflexi surrounding giant protist was significantly lower than the plain sediment, suggesting that bacteria in the activity state were distributed heterogeneously in the geologically static environment by coexistence with the large-sized creature., Competitive research funding, url
- 2007, Principal investigator, 東京大学学術研究活動等奨励事業（国外）学術奨励費, 堀沙耶香, 東京大学, 東京大学学術研究活動等奨励事業（国外）学術奨励費, rm:presentations
- 2007, Principal investigator, 堀沙耶香, 東京大学, 東京大学学術研究活動等奨励事業（国外）学術奨励費, 0, 0, 0, Competitive research funding
■Ⅲ．社会連携活動実績
１．公的団体の委員等（審議会、国家試験委員、他大学評価委員，科研費審査委員等）
- 虫の集い, 線虫提供検討チーム, Feb. 2024 - Present, Society
- JNS, Future Planning Committee - Member of Working Group for JNS Official Journals, Feb. 2020 - Present, Society
- 奈良女子大学, オープンキャンパス委員, Apr. 2024 - Mar. 2026
- 奈良女子大学, 広報委員, Apr. 2024 - Mar. 2026
- 日本神経科学学会, 評議員, Apr. 2023 - Mar. 2026, Society
- 奈良女子大学, 1学年担任, Apr. 2024 - Mar. 2025
- 東京女子医科大学, AI・データサイエンスと医療の委員会 委員, Apr. 2022 - Mar. 2023
- 東京女子医科大学, 看護学部学友会特別会員, Apr. 2021 - Mar. 2023
- 東京女子医科大学, 国家試験対策委員会, Apr. 2021 - Mar. 2023
- 東京女子医科大学, 動物実験委員会, Apr. 2021 - Mar. 2023
- 東京女子医科大学, 実験動物中央施設運営委員会, Apr. 2021 - Mar. 2023
- 東京女子医科大学, ICT(Information and Communication Technology) 委員, Apr. 2021 - Mar. 2023
- 東京女子医科大学, 第１学年教育委員会, Apr. 2021 - Mar. 2023
- 東京女子医科大学, 教学IR委員 支援員, Apr. 2021 - Mar. 2023
- 東京女子医科大学, 数理データサイエンスAI教育プログラム 支援員, Dec. 2021 - Mar. 2022
