(Faculty Division of Human Life and Environmental Sciences Research Group of Food Science and Nutrition)｜Researchers' Profile Teacher performance management system

Name

Author

Position

Affiliation

Research Areas

NAKATA Rieko

Faculty Division of Human Life and Environmental Sciences Research Group of Food Science and Nutrition

Professor

Last Updated :2025/04/27

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Profile Information

Name (Japanese)
Nakata
Name (Kana)
Rieko

Degree

(BLANK), Nara Women's University

(BLANK), Nara Women's University

Research Interests

食品機能成分

ビタミン

葉酸

栄養

Research Areas

Life sciences, Nutrition and health science

Humanities & social sciences, Home economics, lifestyle science

Research History

1994, -:奈良女子大学生活環境学部講師

Professional Memberships

日本肥満学会

日本栄養改善学会

日本脂質生化学会

日本ビタミン学会

日本家政学会

日本栄養・食糧学会

日本生化学会

■Ⅱ．研究活動実績

Published Papers

Biochimica et biophysica acta. Gene regulatory mechanisms, Inflammation-induced nitric oxide suppresses PPARα expression and function via downregulation of Sp1 transcriptional activity in adipocytes., Jungin Kwon; Yumeko Aoki; Haruya Takahashi; Rieko Nakata; Satoko Kawarasaki; Zheng Ni; Rina Yu; Hiroyasu Inoue; Kazuo Inoue; Teruo Kawada; Tsuyoshi Goto, The activation of peroxisome proliferator-activated receptor alpha (PPARα), a ligand-dependent transcription factor that regulates lipid oxidation-related genes, has been employed to treat hyperlipidemia. Emerging evidence indicates that Ppara gene expression decreases in adipose tissue under obese conditions; however, the underlying molecular mechanisms remain elusive. Here, we demonstrate that nitric oxide (NO) suppresses Ppara expression by regulating its promoter activity via suppression of specificity protein 1 (Sp1) transcriptional activity in adipocytes. NO derived from lipopolysaccharide (LPS) -activated macrophages or a NO donor (NOR5) treatment, suppressed Ppara mRNA expression in 10T1/2 adipocytes. In addition, Ppara transcript levels were reduced in the white adipose tissue (WAT) in both acute and chronic inflammation mouse models; however, such suppressive effects were attenuated via a nitric oxide synthase 2 (NOS2) inhibitor. Endoplasmic reticulum (ER) stress inhibitors attenuated the NO-induced repressive effects on Ppara gene expression in 10T1/2 adipocytes. Promoter mutagenesis and chromatin immunoprecipitation assays revealed that NO decreased the Sp1 occupancy in the proximal promoter regions of the Ppara gene, which might partially result from the reduced Sp1 expression levels by NO. This study delineated the molecular mechanism that modulates Ppara gene transcription upon NO stimulation in white adipocytes, suggesting a possible mechanism for the transcriptional downregulation of Ppara in WAT under obese conditions., 20 Sep. 2023, 1866, 4, 194987, 194987, Scientific journal, True, 10.1016/j.bbagrm.2023.194987
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May 2022, 76回, 306, 306

Mar. 2022, 97, 5, 1362, 1362
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Mar. 2022, 97, 5, 1362, 1362
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Aug. 2021, 68回, 87, 87

Current developments in nutrition, Time-Restricted Feeding Regulates Circadian Rhythm of Murine Uterine Clock., Takashi Hosono; Masanori Ono; Takiko Daikoku; Michihiro Mieda; Satoshi Nomura; Kyosuke Kagami; Takashi Iizuka; Rieko Nakata; Tomoko Fujiwara; Hiroshi Fujiwara; Hitoshi Ando, Background: Skipping breakfast is associated with dysmenorrhea in young women. This suggests that the delay of food intake in the active phase impairs uterine functions by interfering with circadian rhythms. Objectives: To examine the relation between the delay of feeding and uterine circadian rhythms, we investigated the effects of the first meal occasion in the active phase on the uterine clock. Methods: Zeitgeber time (ZT) was defined as ZT0 (08:45) with lights on and ZT12 (20:45) with lights off. Young female mice (8 wk of age) were divided into 3 groups: group I (ad libitum consumption), group II (time-restricted feeding during ZT12-16, initial 4 h of the active period), and group III (time-restricted feeding during ZT20-24, last 4 h of the active period, a breakfast-skipping model). After 2 wk of dietary restriction, mice in each group were killed at 4-h intervals and the expression profiles of uterine clock genes, Bmal1 (brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein 1), Per1 (period circadian clock 1), Per2, and Cry1 (cryptochrome 1), were examined. Results: qPCR and western blot analyses demonstrated synchronized circadian clock gene expression within the uterus. Immunohistochemical analysis confirmed that BMAL1 protein expression was synchronized among the endometrium and myometrium. In groups I and II, mRNA expression of Bmal1 was elevated after ZT12 at the start of the active phase. In contrast, Bmal1 expression was elevated just after ZT20 in group III, showing that the uterine clock rhythm had shifted 8 h backward. The changes in BMAL1 protein expression were confirmed by western blot analysis. Conclusions: This study is the first to indicate that time-restricted feeding regulates a circadian rhythm of the uterine clock that is synchronized throughout the uterine body. These findings suggest that the uterine clock system is a new candidate to explain the etiology of breakfast skipping-induced uterine dysfunction., May 2021, 5, 5, nzab064, Scientific journal, True, 10.1093/cdn/nzab064
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Refereed, Nutrients, Adolescent Dietary Habit-induced Obstetric and Gynecologic Disease (ADHOGD) as a New Hypothesis-Possible Involvement of Clock System., Tomoko Fujiwara; Masanori Ono; Michihiro Mieda; Hiroaki Yoshikawa; Rieko Nakata; Takiko Daikoku; Naomi Sekizuka-Kagami; Yoshiko Maida; Hitoshi Ando; Hiroshi Fujiwara, There are growing concerns that poor dietary behaviors at young ages will increase the future risk of chronic diseases in adulthood. We found that female college students who skipped breakfast had higher incidences of dysmenorrhea and irregular menstruation, suggesting that meal skipping affects ovarian and uterine functions. Since dysmenorrhea is more prevalent in those with a past history of dieting, we proposed a novel concept that inadequate dietary habits in adolescence become a trigger for the subsequent development of organic gynecologic diseases. Since inadequate feeding that was limited during the non-active phase impaired reproductive functions in post-adolescent female rats, we hypothesize that circadian rhythm disorders due to breakfast skipping disrupts the hypothalamic-pituitary-ovarian axis, impairs the reproductive rhythm, and leads to ovarian and uterine dysfunction. To explain how reproductive dysfunction is memorized from adolescence to adulthood, we hypothesize that the peripheral clock system also plays a critical role in the latent progression of reproductive diseases together with the central system, and propose naming this concept "adolescent dietary habit-induced obstetric and gynecologic disease (ADHOGD)". This theory will contribute to analyzing the etiologies of and developing prophylaxes for female reproductive diseases from novel aspects. In this article, we describe the precise outline of the above hypotheses with the supporting evidence in the literature., 02 May 2020, 12, 5, Scientific journal, True, 10.3390/nu12051294
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Refereed, Current developments in nutrition, Time Restriction of Food Intake During the Circadian Cycle Is a Possible Regulator of Reproductive Function in Postadolescent Female Rats., Fujiwara T; Nakata R; Ono M; Mieda M; Ando H; Daikoku T; Fujiwara H, Background: We previously reported that skipping breakfast is associated with menstrual disorders of female college students during postadolescent maturation. Objective: In this study, we investigated the effects of meal timing during circadian cycle on the ovarian function using young female rats. Methods: Considering that rats are nocturnally active, 8-wk-old female Wistar rats were classified into 3 groups: Fed during the daytime only (nonactive phase), night-time only (active phase), or control group I (without time or calorie restriction, free access to a standard caloric diet, 20.0% protein, 62.9% carbohydrate, and 7.0% fat, 3.95 kcal/g) for 4 wk. The changes in body weight and frequency of ovulation in each group were evaluated by a weight scale and a vaginal smear, respectively. At the end of the period of dietary restriction, ovaries were removed, and the numbers of growing follicles (mean diameter >250 μm) and corpora lutea (>600 μm) were examined using hematoxylin-eosin-stained tissue sections. In addition, 8-wk-old female rats were fed only during the night-time for 4 wk under a 20%-reduced food supply of the control group II (without any restriction). Results: In the daytime-fed group, the frequency and number of ovulations were significantly decreased compared with those in the control group I (P < 0.05), with a reduced body weight gain concomitant with about 20% of reduction in the daily food intake. In contrast, in the night-time-fed group, even when a 20% reduction in the daily food intake was loaded, their estrus cyclicity did not change despite significant reductions in weight gain and food intake compared with control group II. Conclusion: These findings indicate that restricting food intake to the inactive phase impairs ovarian function in postadolescent female rats, suggesting that the timing of food intake during circadian cycle is one of the crucial factors interfering with the reproductive function., Apr. 2019, 3, 4, nzy093, Scientific journal, 10.1093/cdn/nzy093
DOI URL Scopus URL Scopus Citedby URL

Refereed, Journal of Functional Foods, Lactobacillus helveticus-MIKI-020 enhances hepatic FGF21 expression and decreases the core body temperature during sleep in mice, Kiriyama K; Goto T; Yamamoto H; Ara T; Takahashi H; Jheng HF; Nomura W; Inoue H; Nakata R; Kawada T, Mar. 2019, 54, 529, 535

Refereed, Molecular Nutrition and Food Research, Wiley-VCH Verlag, A Phytol-Enriched Diet Activates PPAR-α in the Liver and Brown Adipose Tissue to Ameliorate Obesity-Induced Metabolic Abnormalities, Ji-Yeong An; Huei-Fen Jheng; Hiroyuki Nagai; Kohei Sanada; Haruya Takahashi; Mari Iwase; Natsumi Watanabe; Young-Il Kim; Aki Teraminami; Nobuyuki Takahashi; Rieko Nakata; Hiroyasu Inoue; Shigeto Seno; Hideo Mastuda; Teruo Kawada; Tsuyoshi Goto, Scope: Peroxisome proliferator-activated receptor alpha (PPAR-α) is a ligand-activated transcription factor that regulates lipid and carbohydrate metabolism. We investigate the effects of naturally occurring PPAR-α agonists, phytol, and its metabolite phytanic acid, on obesity-induced metabolic disorders using a mouse model. Methods and results: A luciferase reporter assay shows that phytanic acid potently activates PPAR-α among PPAR subtypes. In high-fat-diet-induced, severely obese mice, a phytol-enriched diet increases phytanic acid levels in the liver and adipose tissue, where PPAR-α is abundantly expressed. A phytol-enriched diet ameliorates severe obesity and the related metabolic abnormalities of white adipose tissue. Moreover, the expression of PPAR-α target genes in the liver and brown adipose tissue is enhanced by a phytol-enriched diet, suggesting that phytol and phytanic acid activate PPAR-α in these organs. We confirm that phytanic acid treatment induced PPAR-α target gene expression in both primary hepatocytes and brown adipocytes from wild-type mice, but not in these cells from PPAR-α-deficient mice. Conclusion: A phytol-enriched diet may increase phytanic acid levels in the liver and brown adipocytes, thereby activating PPAR-α in these organs and ameliorating obesity-induced metabolic diseases., 01 Mar. 2018, 62, 6, e1700688, Scientific journal, 10.1002/mnfr.201700688
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Refereed, 2018, 90, 4, 529, 532

Seikagaku, Japanese Biochemical Society, Progress in the study on resveratrol, Rieko Nakata; Hiroyasu Inoue, 2018, 90, 4, 529, 532, Scientific journal, 10.14952/SEIKAGAKU.2018.900529
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Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Rice Koji Extract Enhances Lipid Metabolism through Proliferator-Activated Receptor Alpha (PPAR alpha) Activation in Mouse Liver, Haruya Takahashi; Hsin-Yi Chi; Shinsuke Mohri; Kosuke Kamakari; Kelp Nakata; Noriyoshi Ichijo; Rieko Nakata; Hiroyasu Inoue; Tsuyoshi Goto; Teruo Kawada, Koji is made from grains fermented with Aspergillus oiyzae and is essential for the production of many traditional Japanese foods. Many previous studies have shown that koji contributes to the improvement of dyslipidemia. However, little is known regarding the underlying mechanism of this effect. Furthermore, the compound contributing to the activation of lipid metabolism is unknown. We demonstrated that rice koji extract (RKE) induces the mRNA expression of peroxisome proliferator-activated receptor alpha (PPAR alpha) target genes, which promotes lipid metabolism in murine hepatocytes. This effect was not observed in PPAR alpha-KO hepatocytes. We also demonstrated that RKE contained linolenic acid (LIA), oleic acid (OA), and hydroxyoctadecadienoic acids (HODEs), which activate PPAR alpha, using LC-MS analysis. Our findings suggest that RKE, containing LIA, OA, and HODEs, could be valuable in improving dyslipidemia via PPAR alpha activation., Nov. 2016, 64, 46, 8848, 8856, Scientific journal, 10.1021/acs.jafc.6b03516
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Refereed, SCIENTIFIC REPORTS, NATURE PUBLISHING GROUP, Single ingestion of soy beta-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects, Tsutomu Hashidume; Asuka Kato; Tomohiro Tanaka; Shoko Miyoshi; Nobuyuki Itoh; Rieko Nakata; Hiroyasu Inoue; Akira Oikawa; Yuji Nakai; Makoto Shimizu; Jun Inoue; Ryuichiro Sato, Soy protein beta-conglycinin has serum lipid-lowering and anti-obesity effects. We showed that singleingestion of beta-conglycinin after fasting alters gene expression in mouse liver. A sharp increase in fibroblast growth factor 21 (FGF21) gene expression, which is depressed by normal feeding, resulted in increased postprandial circulating FGF21 levels along with a significant decrease in adipose tissue weights. Most increases in gene expressions, including FGF21, were targets for the activating transcription factor 4 (ATF4), but not for peroxisome proliferator-activated receptor a. Overexpression of a dominant-negative form of ATF4 significantly reduced beta-conglycinin-induced increases in hepatic FGF21 gene expression. In FGF21-deficient mice, beta-conglycinin effects were partially abolished. Methionine supplementation to the diet or primary hepatocyte culture medium demonstrated its importance for activating liver or hepatocyte ATF4-FGF21 signaling. Thus, dietary beta-conglycinin intake can impact hepatic and systemic metabolism by increasing the postprandial circulating FGF21 levels., Jun. 2016, 6, 28183, Scientific journal, 10.1038/srep28183
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Refereed, 2016, 3, 5, 424, 426

Refereed, Lipids, Springer Berlin Heidelberg, 9-Oxo-10(E),12(Z),15(Z)-Octadecatrienoic Acid Activates Peroxisome Proliferator-Activated Receptor α in Hepatocytes., Takahashi H; Kamakari K; Goto T; Hara H; Mohri S; Suzuki H; Shibata D; Nakata R; Inoue H; Takahashi N; Kawada T, Sep. 2015, 50, 11, 1083, 1091, 10.1007/s11745-015-4071-3
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Refereed, JOURNAL OF LIPID RESEARCH, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Metabolomics reveal 1-palmitoyl lysophosphatidylcholine production by peroxisome proliferator-activated receptor alpha, Haruya Takahashi; Tsuyoshi Goto; Yota Yamazaki; Kosuke Kamakari; Mariko Hirata; Hideyuki Suzuki; Daisuke Shibata; Rieko Nakata; Hiroyasu Inoue; Nobuyuki Takahashi; Teruo Kawada, PPAR is well known as a master regulator of lipid metabolism. PPAR activation enhances fatty acid oxidation and decreases the levels of circulating and cellular lipids in obese diabetic patients. Although PPAR target genes are widely known, little is known about the alteration of plasma and liver metabolites during PPAR activation. Here, we report that metabolome analysis-implicated upregulation of many plasma lysoGP species during bezafibrate (PPAR agonist) treatment. In particular, 1-palmitoyl lysophosphatidylcholine [LPC(16:0)] is increased by bezafibrate treatment in both plasma and liver. In mouse primary hepatocytes, the secretion of LPC(16:0) increased on PPAR activation, and this effect was attenuated by PPAR antagonist treatment. We demonstrated that Pla(2)g7 gene expression levels in the murine hepatocytes were increased by PPAR activation, and the secretion of LPC(16:0) was suppressed by Pla(2)g7 siRNA treatment. Interestingly, LPC(16:0) activates PPAR and induces the expression of PPAR target genes in hepatocytes. Furthermore, we showed that LPC(16:0) has the ability to recover glucose uptake in adipocytes induced insulin resistance. These results reveal that LPC(16:0) is induced by PPAR activation in hepatocytes; LPC(16:0) contributes to the upregulation of PPAR target genes in hepatocytes and the recovery of glucose uptake in insulin-resistant adipocytes., Feb. 2015, 56, 2, 254, 265, Scientific journal, 10.1194/jlr.M052464
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Refereed, PloS one, The 4'-hydroxyl group of resveratrol is functionally important for direct activation of PPARα., Yoshie Takizawa; Rieko Nakata; Kiyoshi Fukuhara; Hiroshi Yamashita; Hideo Kubodera; Hiroyasu Inoue, Long-term moderate consumption of red wine is associated with a reduced risk of developing lifestyle-related diseases such as cardiovascular disease and cancer. Therefore, resveratrol, a constituent of grapes and various other plants, has attracted substantial interest. This study focused on one molecular target of resveratrol, the peroxisome proliferator activated receptor α (PPARα). Our previous study in mice showed that resveratrol-mediated protection of the brain against stroke requires activation of PPARα; however, the molecular mechanisms involved in this process remain unknown. Here, we evaluated the chemical basis of the resveratrol-mediated activation of PPARα by performing a docking mode simulation and examining the structure-activity relationships of various polyphenols. The results of experiments using the crystal structure of the PPARα ligand-binding domain and an analysis of the activation of PPARα by a resveratrol analog 4-phenylazophenol (4-PAP) in vivo indicate that the 4'-hydroxyl group of resveratrol is critical for the direct activation of PPARα. Activation of PPARα by 5 μM resveratrol was enhanced by rolipram, an inhibitor of phosphodiesterase (PDE) and forskolin, an activator of adenylate cyclase. We also found that resveratrol has a higher PDE inhibitory activity (IC50 = 19 μM) than resveratrol analogs trans-4-hydroxystilbene and 4-PAP (IC50 = 27-28 μM), both of which has only 4'-hydroxyl group, indicating that this 4'-hydroxyl group of resveratrol is not sufficient for the inhibition of PDE. This result is consistent with that 10 μM resveratrol has a higher agonistic activity of PPARα than these analogs, suggesting that there is a feedforward activation loop of PPARα by resveratrol, which may be involved in the long-term effects of resveratrol in vivo., 2015, 10, 3, e0120865, Scientific journal, True, 10.1371/journal.pone.0120865
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Refereed, 2015, 2, 3, 18, 23

Refereed, ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS, BENTHAM SCIENCE PUBL LTD, Resveratrol Targets in Inflammation, Hiroyasu Inoue; Rieko Nakata, Resveratrol, a constituent of grapes and various other plants, has been an attractive compound for biomedical studies because moderate long-term drinking of red wine is associated with a reduced risk of lifestyle-related diseases, such as cardiovascular diseases and cancer. Resveratrol is as a phytoalexin, cyclooxygenase (COX) suppressor, and an activator of peroxisome proliferator-activated receptor (PPAR) and SIRT1. As a major phytoalexin, resveratrol is produced by plants in response to various environmental stresses, such as pathogens and ultraviolet (UV) radiation, and promotes resistance to these stresses. A similar active ingredient, salicylic acid (SA), is also produced by plants. Aspirin, acetylated SA, is a major nonsteroidal anti-inflammatory drug (NSAID) because it inhibits COX activity in humans. The jasmonic acid (JA) pathway in plants and the COX pathway in humans are both defense systems against environmental stresses and involve lipid mediators derived from phospholipids. We can hypothesize that there is a molecular basis for the mutually beneficial relationship between plants and humans, which is important for understanding the mode of action of resveratrol in inflammation. Here we provide a review of the studies on resveratrol, especially with respect to the role of COX and PPAR in inflammation., 2015, 15, 3, 186, 195, Scientific journal, 10.2174/1871530315666150316120316
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2015, 89, 12, 591, 591, 10.20632/vso.89.12_591_1
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Abstracts of Annual Congress of The Japan Society of Home Economics, The Japan Society of Home Economics, Effect of resveratrol on gene expression in human cardiomyocytes derived from iPS cells, Takizawa Yoshie; Hongo Shouko; Nakata Rieko; Inoue Hiroyasu, 2015, 67, 165, 165, 10.11428/kasei.67.0_165
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Abstracts of Annual Congress of The Japan Society of Home Economics, The Japan Society of Home Economics, Imprrovement of lipid metabolism by soybean-derived components targeted to PPAR activation, Nakata Rieko; Hongo Shouko; Takizawa Yoshie; Inoue Hiroyasu, 2015, 67, 163, 163, 10.11428/kasei.67.0_163
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Abstracts of the Annual Meeting of the Japan Society of Cookery Science, The Japan Society of Cookery Science, The addiction of smartphone usage in young women may affect self-recognition of food intake, Fujiwara Tomoko; Yamagishi Fumino; Nakata Rieko, 2015, 27, 200, 200, 10.11402/ajscs.27.0_200
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Refereed, FEBS LETTERS, ELSEVIER SCIENCE BV, Disturbed biopterin and folate metabolism in the Qdpr-deficient mouse, Feng Xu; Yusuke Sudo; Sho Sanechika; Junpei Yamashita; Sho Shimaguchi; Shun-ichiro Honda; Chiho Sumi-Ichinose; Masayo Mori-Kojima; Rieko Nakata; Tadaomi Furuta; Minoru Sakurai; Masahiro Sugimoto; Tomoyoshi Soga; Kazunao Kondo; Hiroshi Ichinose, Quinonoid dihydropteridine reductase (QDPR) catalyzes the regeneration of tetrahydrobiopterin (BH4), a cofactor for monoamine synthesis, phenylalanine hydroxylation and nitric oxide production. Here, we produced and analyzed a transgenic Qdpr (/) mouse model. Unexpectedly, the BH4 contents in the Qdpr (/) mice were not decreased and even increased in some tissues, whereas those of the oxidized form dihydrobiopterin (BH2) were significantly increased. We demonstrated that unlike the wild-type mice, dihydrofolate reductase regenerated BH4 from BH2 in the mutants. Furthermore, we revealed wide alterations in folate-associated metabolism in the Qdpr (/) mice, which suggests an interconnection between folate and biopterin metabolism in the transgenic mouse model. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved., Nov. 2014, 588, 21, 3924, 3931, Scientific journal, 10.1016/j.febslet.2014.09.004
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Oct. 2014, 87回, [2P, 419]

Refereed, PHOTOCHEMISTRY AND PHOTOBIOLOGY, WILEY-BLACKWELL, Possible Involvement of a Tetrahydrobiopterin in Photoreception for UV-B-induced Anthocyanin Synthesis in Carrot, Junko Takeda; Rieko Nakata; Hiroshi Ueno; Akio Murakami; Mineo Iseki; Masakatsu Watanabe, Our previous studies of action spectra for UV-B-induced anthocyanin accumulation in cultured carrot cells indicated that a reduced form of pterin, possibly tetrahydrobiopterin, contributes to UV-B photoreception. In this report, we provide additional evidence for the involvement of pterin in UV-B light sensing. UV-B-induced phenylalanine ammonia-lyase (PAL) activity was considerably suppressed by N-acetylserotonin (an inhibitor of tetrahydrobiopterin biosynthesis), and this suppression was partially recovered by adding biopterin or tetrahydrobiobiopterin. In addition, protein(s) specifically bound to biopterin were detected by radiolabeling experiments in N-acetylserotonin-treated cells. Furthermore, diphenyleneiodonium, a potent inhibitor of electron transfer, completely suppressed UV-B-induced PAL activity. These results suggest the occurrence of an unidentified UV-B photoreceptor (other than UVR8, the tryptophan-based UV-B sensor originally identified in Arabidopsis) with reduced pterin in carrot cells. After reexamining published action spectra, we suggest that anthocyanin synthesis is coordinately regulated by these two UV-B sensors., Sep. 2014, 90, 5, 1043, 1049, Scientific journal, 10.1111/php.12302
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Refereed, LEUKEMIA & LYMPHOMA, INFORMA HEALTHCARE, Plasma homocysteine, methionine and S-adenosylhomocysteine levels following high-dose methotrexate treatment in pediatric patients with acute lymphoblastic leukemia or Burkitt lymphoma: association with hepatotoxicity, Masaru Kubota; Rieko Nakata; Souichi Adachi; Ken-Ichiro Watanabe; Toshio Heike; Yasufumi Takeshita; Midori Shima, This study aimed to investigate: (i) changes of plasma homocysteine, methionine and S-adenosylhomocysteine levels following high-dose methotrexate (HD-MTX) treatment and (ii) the correlation of these sulfur-containing amino acids with MTX-induced hepatotoxicity. Fifteen pediatric patients with acute lymphoblastic leukemia and one patient with Burkitt lymphoma, with a total of 26 treatment courses of HD-MTX, were enrolled. Homocysteine levels increased at 24 h after HD-MTX treatment, and showed marginal decreases at 48 and 72 h. Methionine levels showed a biphasic pattern, i. e. an initial decrease at 24 h followed by increases at 48 and 72 h. S-adenosylhomocysteine exhibited a marginal decrease at 24 h. Changes of homocysteine exhibited significant correlation only with a maximum increase of alanine aminotransferase or total bilirubin from baseline. This study has demonstrated, for the first time, simultaneous changes of plasma homocysteine, methionine and S-adenosylhomocysteine following HD-MTX. The potential of homocysteine as a marker of hepatotoxicity is also presented., Jul. 2014, 55, 7, 1591, 1595, Scientific journal, 10.3109/10428194.2013.850684
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Not Refereed, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, AMER PHYSIOLOGICAL SOC, Role of AMPK and PPAR gamma 1 in exercise-induced lipoprotein lipase in skeletal muscle, Takashi Sasaki; Rieko Nakata; Hiroyasu Inoue; Makoto Shimizu; Jun Inoue; Ryuichiro Sato, Exercise can effectively ameliorate type 2 diabetes and insulin resistance. Here we show that the mRNA levels of one of peroxisome proliferator-activated receptor (PPAR) family members, PPAR gamma 1, and genes related to energy metabolism, including PPAR gamma coactivator-1 protein-1 alpha (PGC-1 alpha) and lipoprotein lipase (LPL), increased in the gastrocnemius muscle of habitual exercise-trained mice. When mice were intraperitoneally administered an AMP-activated protein kinase (AMPK) activator 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), the mRNA levels of the aforementioned three genes increased in gastrocnemius muscle. AICAR treatment to C2C12 differentiated myotubes also increased PPAR gamma 1 mRNA levels, but not PPAR alpha and -delta mRNA levels, concomitant with increased PGC-1 alpha mRNA levels. An AMPK inhibitor, compound C, blocked these AICAR effects. AICAR treatment increased the half-life of PPAR gamma 1 mRNA nearly threefold (4-12 h) by activating AMPK. When C2C12 myoblast cells infected with a PPAR gamma 1 expression lentivirus were differentiated into myotubes, PPAR gamma 1 overexpression dramatically increased LPL mRNA levels more than 40-fold. In contrast, when PPAR gamma 1 expression was suppressed in C2C12 myotubes, LPL mRNA levels were significantly reduced, and the effect of AICAR on increased LPL gene expression was almost completely blocked. These results indicated that PPAR gamma was intimately involved in LPL gene expression in skeletal muscle and the AMPK-PPAR gamma 1 pathway may play a role in exercise-induced LPL expression. Thus, we identified a novel critical role for PPAR gamma 1 in response to AMPK activation for controlling the expression of a subset of genes associated with metabolic regulation in skeletal muscle., May 2014, 306, 9, E1085, E1092, Scientific journal, 10.1152/ajpendo.00691.2013
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Refereed, Curr Nutr Rep, Resveratrol and Cardiovascular Disease, NAKATA Rieko; Nakata R; Inoue H, 2014, 3, 163, 169

2014, 88, 2, 108, 109, 10.20632/vso.88.2_108
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Not Refereed, British Journal of Nutrition, Up-regulation of endothelial nitric oxide synthase (eNOS), silent mating type information regulation 2 homologue 1 (SIRT1) and autophagy-related genes by repeated treatments with resveratrol in human umbilical vein endothelial cells, Yoshie Takizawa; Yukiko Kosuge; Hiroyo Awaji; Emi Tamura; Ayako Takai; Takaaki Yanai; Reiko Yamamoto; Koichi Kokame; Toshiyuki Miyata; Rieko Nakata; Hiroyasu Inoue, Resveratrol, a polyphenolic phytoalexin found in red wine and various plants, has been reported to up-regulate the expression of endothelial NO synthase (eNOS) in human umbilical vein endothelial cells (HUVEC). However, this effect was neither long term in nature nor physiologically relevant at the concentration of resveratrol studied. In the present study, we investigated the effects of repeated treatments with a lower concentration of resveratrol on the expression of genes in HUVEC. The expression levels of eNOS and silent mating type information regulation 2 homologue 1 (SIRT1) were up-regulated in HUVEC by repeated treatments with 1 μm-resveratrol for 6 d, but not with fenofibrate. Moreover, resveratrol treatment increased the expression of autophagy-regulated genes such as γ-aminobutyric acid A receptor-associated protein (GABARAP), microtubule-associated protein 1 light chain 3B (LC3B) and autophagy-related protein 3 (ATG3), the radical scavenger activity-related metallothionein-1X (MT1X) gene and the anti-inflammatory activity-related annexin A2 (ANXA) gene. In addition, resveratrol treatment down-regulated the expression of the cell-cycle checkpoint control RAD9 homologue B (RAD9B) gene. These results indicate the beneficial effects of resveratrol on the cardiovascular system. © The Authors 2013., 28 Dec. 2013, 110, 12, 2150, 2155, Scientific journal, 10.1017/S0007114513001670
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Not Refereed, JOURNAL OF LIPID RESEARCH, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, DHA attenuates postprandial hyperlipidemia via activating PPAR alpha in intestinal epithelial cells, Rino Kimura; Nobuyuki Takahashi; Shan Lin; Tsuyoshi Goto; Kaeko Murota; Rieko Nakata; Hiroyasu Inoue; Teruo Kawada, It is known that peroxisome proliferator-activated receptor (PPAR)alpha, whose activation reduces hyperlipidemia, is highly expressed in intestinal epithelial cells. Docosahexaenoic acid (DHA) could improve postprandial hyperlipidemia, however, its relationship with intestinal PPAR alpha activation is not revealed. In this study, we investigated whether DHA can affect postprandial hyperlipidemia by activating intestinal PPAR alpha using Caco-2 cells and C57BL/6 mice. The genes involved in fatty acid (FA) oxidation and oxygen consumption rate were increased, and the secretion of triacylglyceride (TG) and apolipoprotein B (apoB) was decreased in DHA-treated Caco-2 cells. Additionally, intestinal FA oxidation was induced, and TG and apoB secretion from intestinal epithelial cells was reduced, resulting in the attenuation of plasma TG and apoB levels after oral administration of olive oil in DHA-rich oil-fed mice compared with controls. However, no increase in genes involved in FA oxidation was observed in the liver. Furthermore, the effects of DHA on intestinal lipid secretion and postprandial hyperlipidemia were abolished in PPAR alpha knockout mice. In conclusion, the present work suggests that DHA can inhibit the secretion of TG from intestinal epithelial cells via PPAR alpha activation, which attenuates postprandial hyperlipidemia., Dec. 2013, 54, 12, 3258, 3268, Scientific journal, 10.1194/jlr.M034942
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Not Refereed, FEBS LETTERS, ELSEVIER SCIENCE BV, Secretion of miraculin through the function of a signal peptide conserved in the Kunitz-type soybean trypsin inhibitor family, Ayako Takai; Makiko Satoh; Tomomi Matsuyama; Akane Ito; Rieko Nakata; Takashi Aoyama; Hiroyasu Inoue, Miraculin, a glycoprotein that modifies sour tastes into sweet ones, belongs to the Kunitz-type soybean trypsin inhibitor (STI) family. To clarify the functional relation of miraculin with Kunitz-type STIs, we investigated its subcellular localization and trypsin inhibitory activity. In transgenic Arabidopsis thaliana, miraculin, fused to yellow fluorescent protein, localized to and outside the plasma membrane depending on the putative secretion signal peptide. When transgenic seedlings were cultured in liquid medium, miraculin was present in the supernatant only after cellulase treatment. No trypsin inhibitory activity was detected in native or recombinant miraculin. In conclusion, miraculin is secreted outside the plasma membrane through the function of a signal peptide, conserved in Kunitz-type STIs, whereas its trypsin inhibitory activity may be lost during its evolution. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved., Jun. 2013, 587, 12, 1767, 1772, Scientific journal, 10.1016/j.febslet.2013.04.026
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Not Refereed, Food Science and Technology Research, Evaluation of food-derived functional ingredients according to activation of PPAR and suppression of COX-2 expression, Rieko Nakata; Yoshie Takizawa; Ayako Takai; Hiroyasu Inoue, The peroxisome proliferator-activated receptors (PPARs) are ligand-dependent transcription factors belonging to the nuclear receptor family. They are considered molecular targets for the prevention of lifestyle-related diseases and are involved in the control of cyclooxygenase (COX)-2 expression. COX-2, the rate-limiting enzyme in prostaglandin biosynthesis, plays a key role in inflammation and circulatory homeostasis, and its expression is partly controlled by PPAR. We have identified several natural chemicals, such as resveratrol, that activate PPARs and suppress COX-2 expression. In this review, we provide an evaluation of food-derived functional ingredients that target PPARs and COX-2., 2013, 19, 3, 339, 345, 10.3136/fstr.19.339
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Not Refereed, 2013, 56, 544, 546

Abstracts of the Annual Meeting of the Japan Society of Cookery Science, The Japan Society of Cookery Science, The effects of the caloric restriction on ovarian function in post-adolescent women, Fujiwara Tomoko; Furukawa Azusa; Matsumoto Akane; Nakata Rieko, 2013, 25, 136, 136, 10.11402/ajscs.25.0.136.0
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Abstracts of Annual Congress of The Japan Society of Home Economics, The Japan Society of Home Economics, Improvement of lipid metabolism by cinnamon burk oil, Takizawa Yoshie; Nakata Rieko; Inoue Hiroyasu, 2013, 65, 78, 78, 10.11428/kasei.65.0_78
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Abstracts of Annual Congress of The Japan Society of Home Economics, The Japan Society of Home Economics, Effect of folate deficiency on lipid metabolism in high-fat diet, NAKATA Rieko; MATSUMOTO Akane; INOUE Hiroyasu, 2013, 65, 79, 79, 10.11428/kasei.65.0_79
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Abstracts of Annual Congress of The Japan Society of Home Economics, The Japan Society of Home Economics, Possible effects of dietary intention on reproductive function of young women, FUJIWARA Tomoko; NAKATA Rieko, 2013, 65, 148, 148, 10.11428/kasei.65.0_148
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2013, 87, 5, 309, 310, 10.20632/vso.87.5-6_309
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Not Refereed, JOURNAL OF FOOD SCIENCE, WILEY-BLACKWELL, The Beneficial Effect of Folate-Enriched Egg on the Folate and Homocysteine Levels in Rats Fed a Folate- and Choline-Deficient Diet, Ayami Sugiyama; Hiroyo Awaji; Kenji Horie; Mujo Kim; Rieko Nakata, We investigated the effects of folate-enriched egg yolk powder on folate and homocysteine levels in plasma and liver of rats fed the folate- and choline-deficient diet to determine bioavailability in vivo. Three-wk-old Wistar rats were fed (1) the pteroylglutamate (PteGlu), (2) the choline, (3) the PteGlu and choline, (4) the folate-enriched egg yolk powder diet for 4 wk after having been fed the folate-and choline-deficient diet. The hepatic folate level in the folate-enriched egg yolk powder group was significantly higher than that in the folate-and choline-deficient or the control groups. The homocysteine concentration in plasma and liver of the folate-enriched egg yolk powder group was significantly lower than that of the folate-and choline-deficient or the PteGlu groups. The S-adenosyl-methionine (SAM)/S-adenosyl-homocysteine (SAH) ratio in the folate-enriched egg yolk powder group was significantly higher than that in the folate-and choline-deficient group. These effects were similar in the PteGlu and choline, but not the PteGlu or the choline groups. These data suggest that the intake of folate-enriched eggs, as well as of both folate and choline, induced the beneficial effects on folate and homocysteine metabolism. Thus, folate-enriched eggs could be used as beneficial source of folate with a high bioavailability., Dec. 2012, 77, 12, H268, H272, Scientific journal, 10.1111/j.1750-3841.2012.02997.x
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Not Refereed, BIOLOGICAL & PHARMACEUTICAL BULLETIN, PHARMACEUTICAL SOC JAPAN, Recent Advances in the Study on Resveratrol, Rieko Nakata; Satoru Takahashi; Hiroyasu Inoue, Appropriate long-term drinking of red wine is associated with a reduced risk for lifestyle-related diseases such as cardiovascular disease and cancer, making resveratrol, a constituent of grapes and various other plants, an attractive compound to be studied. Historically, resveratrol has been identified as a phytoalexin, antioxidant, cyclooxygenase (COX) inhibitor, peroxisome proliferator-activated receptor (PPAR) activator, endothelial nitric oxide synthase (eNOS) inducer, silent mating type information regulation 2 homolog I (SIRT1) activator, and more. Despite scepticism concerning the biological availability of resveratrol, a growing body of in viva evidence indicates that resveratrol has protective effects in several stress and disease models. Here, we provide a review of the studies on resveratrol, especially with respect to COX, PPAR, and eNOS activities, and discuss its potential for promoting human health., Mar. 2012, 35, 3, 273, 279, 10.1248/bpb.35.273
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Not Refereed, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, AMER PHYSIOLOGICAL SOC, Farnesol, an isoprenoid, improves metabolic abnormalities in mice via both PPAR alpha-dependent and -independent pathways, Tsuyoshi Goto; Young-Il Kim; Kozue Funakoshi; Aki Teraminami; Taku Uemura; Shizuka Hirai; Joo-Young Lee; Makoto Makishima; Rieko Nakata; Hiroyasu Inoue; Hiroyuki Senju; Masayoshi Matsunaga; Fumihiko Horio; Nobuyuki Takahashi; Teruo Kawada, Goto T, Kim YI, Funakoshi K, Teraminami A, Uemura T, Hirai S, Lee JY, Makishima M, Nakata R, Inoue H, Senju H, Matsunaga M, Horio F, Takahashi N, Kawada T. Farnesol, an isoprenoid, improves metabolic abnormalities in mice via both PPAR alpha-dependent and -independent pathways. Am J Physiol Endocrinol Metab 301: E1022-E1032, 2011. First published August 23, 2011; doi:10.1152/ajpendo.00061.2011.-Peroxisome proliferator-activated receptors (PPARs) control energy homeostasis. In this study, we showed that farnesol, a naturally occurring ligand of PPARs, could ameliorate metabolic diseases. Obese KK-Ay mice fed a high-fat diet (HFD) containing 0.5% farnesol showed significantly decreased serum glucose level, glucosuria incidence, and hepatic triglyceride contents. Farnesol-containing HFD upregulated the mRNA expressions of PPAR alpha target genes involved in fatty acid oxidation in the liver. On the other hand, farnesol was not effective in upregulating the mRNA expressions of PPAR gamma target genes in white adipose tissues. Experiments using PPAR alpha-deficient [ (-/-)] mice revealed that the upregulation of fatty acid oxidation-related genes required PPAR alpha function, but the suppression of hepatic triglyceride accumulation was partially PPAR alpha dependent. In hepatocytes isolated from the wild-type and PPAR alpha (-/-) mice, farnesol suppressed triglyceride synthesis. In luciferase assay, farnesol activated both PPAR alpha and the farnesoid X receptor (FXR) at similar concentrations. Moreover, farnesol increased the mRNA expression level of a small heterodimer partner known as one of the FXR target genes and decreased those of sterol regulatory element-binding protein-1c and fatty acid synthase in both the wildtype and PPAR alpha (-/-) hepatocytes. These findings suggest that farnesol could improve metabolic abnormalities in mice via both PPAR alpha-dependent and -independent pathways and that the activation of FXR by farnesol might contribute partially to the PPAR alpha-independent hepatic triglyceride content-lowering effect. To our knowledge, this is the first study on the effect of the dual activators of PPAR alpha and FXR on obesity-induced metabolic disorders., Nov. 2011, 301, 5, E1022, E1032, Scientific journal, 10.1152/ajpendo.00061.2011
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Not Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, Citronellol and Geraniol, Components of Rose Oil, Activate Peroxisome Proliferator-Activated Receptor alpha and gamma and Suppress Cyclooxygenase-2 Expression, Michiko Katsukawa; Rieko Nakata; Satomi Koeji; Kazuyuki Hori; Saori Takahashi; Hiroyasu Inoue, We evaluated the effects of rose oil on the peroxisome proliferator-activated receptor (PPAR) and cyclooxygenase-2 (COX-2). Citronellol and geraniol, the major components of rose oil, activated PPAR alpha and gamma, and suppressed LPS-induced COX-2 expression in cell culture assays, although the PPAR gamma-dependent suppression of COX-2 promoter activity was evident only with citronellol, indicating that citronellol and geraniol were the active components of rose oil., May 2011, 75, 5, 1010, 1012, Scientific journal, 10.1271/bbb.110039
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Not Refereed, APPETITE, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Skipping breakfast is associated with reproductive dysfunction in post-adolescent female college students, Tomoko Fujiwara; Rieko Nakata, Although increasing attention has been paid to the adverse effects of skipping breakfast on quality of life, there are very few reports concerning the relationship between skipping breakfast and reproductive function. Therefore, we examined this issue by conducting a questionnaire survey of female college students aged from 18 to 20 years old. The 5 annual surveys of questionnaire demonstrated that the severity of dysmenorrhea was significantly higher in the population that skipped breakfast. The incidence of irregular menses was also higher in the population that skipped breakfast, although there was no difference in the incidence of premenstrual symptoms. The group that skipped breakfast showed a tendency to suffer from constipation. In addition, despite no difference in body mass index, there was a significantly higher incidence of a self-perception of poor general health among the group that skipped breakfast. These findings suggest that skipping breakfast is associated with menstrual disorders, and affects the physical condition of female college students who are undergoing post-adolescent maturation. Since these menstrual disorders may influence the quality of life of young women not only in the present but also in the future, skipping breakfast should be re-evaluated from the perspective of future reproductive function. (C) 2010 Elsevier Ltd. All rights reserved., Dec. 2010, 55, 3, 714, 717, Scientific journal, 10.1016/j.appet.2010.08.005
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Not Refereed, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, ELSEVIER SCIENCE BV, Citral, a component of lemongrass oil, activates PPAR alpha and gamma and suppresses COX-2 expression, Michiko Katsukawa; Rieko Nakata; Yoshie Takizawa; Kazuyuki Hori; Saori Takahashi; Hiroyasu Inoue, Lemongrass is a widely used herb as a food flavoring, as a perfume, and for its analgesic and anti-inflammatory purposes; however, the molecular mechanisms of these effects have not been elucidated. Previously, we identified carvacrol from the essential oil of thyme as a suppressor of cyclooxygenase (COX)-2, a key enzyme for prostaglandin synthesis, and also an activator of peroxisome proliferator-activated receptor (PPAR), a molecular target for "lifestyle-related" diseases. In this study, we evaluated the essential oil of lemongrass using our established assays for COX-2 and PPARs. We found that COX-2 promoter activity was suppressed by lemongrass oil in cell-based transfection assays, and we identified citral as a major component in the suppression of COX-2 expression and as an activator of PPAR alpha and gamma. PPAR gamma-dependent suppression of COX-2 promoter activity was observed in response to citral treatment In human macrophage-like U937 cells, citral suppressed both U'S-induced COX-2 mRNA and protein expression, dose-dependently. Moreover, citral induced the mRNA expression of the PPAR alpha-responsive carnitine palmitoyltransferase 1 gene and the PPAR gamma-responsive fatty acid binding protein 4 gene, suggesting that citral activates PPAR alpha and gamma, and regulates COX-2 expression. These results are important for understanding the anti-inflammatory and anti-lifestyle-related disease properties of lemongrass. (C) 2010 Elsevier B.V. All rights reserved., Nov. 2010, 1801, 11, 1214, 1220, Scientific journal, 10.1016/j.bbalip.2010.07.004
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Not Refereed, NUTRITION & METABOLISM, BIOMED CENTRAL LTD, Vaticanol C, a resveratrol tetramer, activates PPAR alpha and PPAR beta/delta in vitro and in vivo, Tomoko Tsukamoto; Rieko Nakata; Emi Tamura; Yukiko Kosuge; Aya Kariya; Michiko Katsukawa; Satoshi Mishima; Tetsuro Ito; Munekazu Iinuma; Yukihiro Akao; Yoshinori Nozawa; Yuji Arai; Shobu Namura; Hiroyasu Inoue, Background: Appropriate long-term drinking of red wine is associated with a reduced risk of cardiovascular disease. Resveratrol, a well-known SIRT1 activator is considered to be one of the beneficial components contained in red wine, and also developed as a drug candidate. We previously demonstrated that resveratrol protects brain against ischemic stroke in mice through a PPAR alpha-dependent mechanism. Here we report the different effects of the oligomers of resveratrol.
Methods: We evaluated the activation of PPARs by epsilon-viniferin, a resveratrol dimer, and vaticanol C, a resveratrol tetramer, in cell-based reporter assays using bovine arterial endothelial cells, as well as the activation of SIRT1. Moreover, we tested the metabolic action by administering vaticanol C with the high fat diet to wild-type and PPAR alpha-knockout male mice for eight weeks.
Results: We show that vaticanol C activates PPAR alpha and PPAR beta/delta in cell-based reporter assays, but does not activate SIRT1. epsilon-Viniferin shows a similar radical scavenging activity as resveratrol, but neither effects on PPARs and SIRT-1. Eight-week intake of vaticanol C with a high fat diet upregulates hepatic expression of PPAR alpha-responsive genes such as cyp4a10, cyp4a14 and FABP1, and skeletal muscle expression of PPAR beta/delta-responsive genes, such as UCP3 and PDK4 ( pyruvate dehydrogenase kinase, isoform 4), in wild-type, but not PPAR alpha-knockout mice.
Conclusion: Vaticanol C, a resveratrol tetramer, activated PPAR alpha and PPAR beta/delta in vitro and in vivo. These findings indicate that activation of PPAR alpha and PPAR beta/delta by vaticanol C may be a novel mechanism, affording beneficial effects against lifestyle-related diseases., May 2010, 7, 46, Scientific journal, 10.1186/1743-7075-7-46
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Not Refereed, JOURNAL OF LIPID RESEARCH, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Carvacrol, a component of thyme oil, activates PPAR alpha and gamma and suppresses COX-2 expression, Mariko Hotta; Rieko Nakata; Michiko Katsukawa; Kazuyuki Hori; Saori Takahashi; Hiroyasu Inoue, Cyclooxygenase-2 (COX-2), the rate-limiting enzyme in prostaglandin biosynthesis, plays a key role in inflammation and circulatory homeostasis. Peroxisome proliferator-activated receptors (PPARs) are ligand-dependent transcription factors belonging to the nuclear receptor superfamily and are involved in the control of COX-2 expression, and vice versa. Here, we show that COX-2 promoter activity was suppressed by essential oils derived from thyme, clove, rose, eucalyptus, fennel, and bergamot in cell-based transfection assays using bovine arterial endothelial cells. Moreover, from thyme oil, we identified carvacrol as a major component of the suppressor of COX-2 expression and an activator of PPAR alpha and gamma. PPAR gamma-dependent suppression of COX-2 promoter activity was observed in response to carvacrol treatment. In human macrophage-like U937 cells, carvacrol suppressed lipopolysaccharide-induced COX-2 mRNA and protein expression, suggesting that carvacrol regulates COX-2 expression through its agonistic effect on PPAR alpha. These results may be important in understanding the antiinflammatory and antilifestyle-related disease properties of carvacrol.-Hotta, M., R. Nakata, M. Katsukawa, K. Hori, S. Takahashi, and H. Inoue. Carvacrol, a component of thyme oil, activates PPAR alpha and gamma and suppresses COX-2 expression. J. Lipid Res. 2010. 51: 132-139., Jan. 2010, 51, 1, 132, 139, Scientific journal, 10.1194/jlr.M900255-JLR200
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Not Refereed, 2010, 17, 203, 206

Not Refereed, JOURNAL OF BIOCHEMISTRY, OXFORD UNIV PRESS, Functional Expression of Miraculin, a Taste-Modifying Protein in Escherichia Coli, Tomomi Matsuyama; Makiko Satoh; Rieko Nakata; Takashi Aoyama; Hiroyasu Inoue, Miraculin isolated from red berries of Richadella dulcifica, a native shrub of West Africa, has the unusual property of modifying a sour taste into a sweet one. This homodimer protein consists of two glycosylated polypeptides that are cross-linked by a disulfide bond. Recently, functional expression of miraculin was reported in host cells with the ability to glycosylate proteins, such as lettuce, tomato and the microbe Aspergillus oryzae, but not Escherichia coli. Thus, a question remains as to whether glycosylation of miraculin is essential for its taste-modifying properties. Here we show that recombinant miraculin expressed in E. coli has taste-modifying properties as a homodimer, not as a monomer, indicating that glycosylation is not essential for the taste-modifying property., Apr. 2009, 145, 4, 445, 450, Scientific journal, 10.1093/jb/mvn184
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Not Refereed, INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, TAYLOR & FRANCIS LTD, Skipping breakfast adversely affects menstrual disorders in young college students, Tomoko Fujiwara; Natsuyo Sato; Hiroyo Awaji; Hiroko Sakamoto; Rieko Nakata, In the present study we conducted a questionnaire survey to examine the relationship between dietary habits and menstrual disorders in young women. Subjects were recruited from 315 college students and were classified as: Group I, eating breakfast; Group II, skipping breakfast; Group III, not eating fast foods; Group IV, eating fast foods; Group V, not eating processed foods; and Group VI, eating processed foods. The intensity of dysmenorrhea was scored using three grades. All participants were further divided into groups based on having regular or irregular menstruation, having premenstrual symptoms or not, and self-perception of good or poor general health. General health was poor in Groups II and VI, and dysmenorrhea scores were high in Groups II, IV and VI. The incidence of irregular menses was also high in Group II. However, there was no apparent relation between premenstrual symptoms and dietary habits. These findings suggest that skipping breakfast adversely affects menstrual disorders in young college students., 2009, 60, S6, 23, 31, Scientific journal, 10.1080/09637480802260998
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Not Refereed, 2009, 16, 293, 294

Not Refereed, 2009, 16, 683, 686

Not Refereed, Journal of Home Economics of Japan, The Japan Society of Home Economics, Evaluation of Plant Oils by Their Suppressive Effects on the Expression of Cyclooxygenase-2, HOTTA Mariko; NAKATA Rieko; INOUE Hiroyasu, Cyclooxygenase-2 (COX-2), a rate-limiting enzyme for prostaglandins (PG), is widely accepted as a target for non-steroidal anti-inflammatory drugs (NSAIDs). Recent studies have shown that COX-2 was involved not only in inflammation, but also in tumorigenesis and lifestyle-related diseases. We have been investigating the regulation of the COX-2 gene, and found that several chemicals derived from plants such as resveratrol (grapes) suppressed COX-2 gene expression. In this present study, 21 plant oils were evaluated for their suppressive effect on COX-2 gene expression by using a transient transfection assay of the luciferase reporter vector containing the human COX-2 promoter into arterial endothelial cells. We found that the COX-2 promoter activity was suppressed by origanum (65%), clove (40%), rose (30%), eucalyptus (25%), fennel (22%) and bergamot oils (21%) in descending order, suggesting that these oils function as suppressors of COX-2 gene expression, and that this assay system would be useful to identify novel functional components in food materials., 2008, 59, 6, 373, 378, 10.11428/jhej.59.373
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Not Refereed, 2008, 15, 3, 517, 520

Not Refereed, The Open Medical Informatics Journal, Young Japanese college students with dysmenorrheal have high frequency of irregular menstruation and premenstryal symptoms., NAKATA Rieko; Fujiwara T; Nakata R, 2007, 1, 8, 11

Not Refereed, The Open Food Science Journal, Adverse effects of dietary habits on menstrual disorders in young women., NAKATA Rieko; Fujiwara T; Sato N; Awaji H; Nakata R, 2007, 1, 24, 30

Not Refereed, NUTRITION RESEARCH, PERGAMON-ELSEVIER SCIENCE LTD, Betaine supplementation suppresses plasma homocysteine level elevation induced by folate deficiency in rats, Masako Yagisawa; Yuko Doi; Terumi Uenohara; Maiko Toda; Norihiro Shigematsu; Rieko Nakata, The effects of betaine on the plasma homocysteine concentration were investigated in partially folate deficient rats. The rats were fed a test diet containing folic acid at a dose of 8 (control) or 0.5 (low folate) mg/kg. After a 4-week feeding, the plasma homocysteine concentration in the low-folate group increased to 1.6-fold that in the control group. Betaine supplementation (30 g/kg diet) significantly suppressed the plasma homocysteine level elevation and led to an upward trend in liver betaine-homocysteine methyltransferase (BHMT) activity, whereas no change was observed with supplementation with choline (10 g/kg diet), a precursor of betaine. The results of this study suggest that betaine suppresses the plasma homocysteine level elevation induced by low folate intake, and the suppression is associated with the metabolic pathway driven by BHMT. (c) 2006 Elsevier Inc. All rights reserved., Jun. 2006, 26, 6, 266, 270, Scientific journal, 10.1016/j.nutres.2006.05.011
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Not Refereed, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, CENTER ACADEMIC PUBL JAPAN, Effects of chronic betaine ingestion on methionine-loading induced plasma homocysteine elevation in rats, Masako Yagisawa; Norihiro Shigematsu; Rieko Nakata, The effects of chronic betaine ingestion were investigated in rats. Rats were fed an experimental diet containing 5% bctaine for 4wk and methionine was intravenously administered. The elevations of plasma homocysteine were assessed by comparing the increments to the initial measured value and the positive incremental area under the plasma homocysteine concentration curve over the 240-min post-methionine-loading period (Delta AUC(0-240)). In the betaine-ingesting rats, Delta AUC(0-240) was significantly lower than in the control group (48% of the control), and the increments of plasma homocysteine were also lower compared with the control, especially 15-30 min after methionine loading. Choline, a precursor of betaine, did not alter the plasma homocysteme elevation. In a definite period immediately after methionine loading, carnitine, a methyl-group-rich amino acid, induced a significant increase of plasma homocysteme, compared to the control. Moreover, plasma homocysteine concentration was significantly decreased by 4 wk of betaine ingestion. Betaine enhanced liver BHMT activity whereas choline and carnitine did not show any effects on BHMT activity. These results suggest that betaine contributes to both the decrease in the plasma homocysteine concentration and the suppression of plasma homocysteine elevation through the activation of liver BHMT., Jun. 2006, 52, 3, 194, 199, Scientific journal, 10.3177/jnsv.52.194
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Not Refereed, Current Trends in Endocrinology, Assessment of human regular menstrual cycle., NAKATA Rieko; Fujiwara T; Nakata R, 2006, 2, 59, 64

Not Refereed, Current Trends in Endocrinology, The influence of food intake on female reproductive function in young women., NAKATA Rieko; Fujiwara T; Nakata R; Fujiwara H, 2005, 1, 137, 143

Not Refereed, JOURNAL OF NUTRITIONAL BIOCHEMISTRY, ELSEVIER SCIENCE INC, Effects of intravenous betaine on methionine-loading-induced plasma homocysteine elevation in rats, M Yagisawa; N Okawa; N Shigematsu; R Nakata, An intravenous methionine-loading model was characterized, and the suppressive effect of betaine on plasma homocysteine elevation induced by methionine loading was examined in rats. The plasma homocysteine concentrations significantly increased 5-120 minutes after 0.34 mmol/kg of methionine loading and then returned to the baseline within 240 minutes. Betaine was then intravenously administered at the same time as the methionine loading. The total increment of plasma homocysteine was assessed using the positive incremental area under the plasma homocysteine concentration curve over the 240-minute post-methionine-loading period (DeltaAUC(0-240)).
Betaine reduced DeltaAUC(0-240) dose-dependently: 81% of the control by 1.7 mmol/kg of betaine and 33% by 3.4 mmol/kg. The effects of glycine and methylglycine, analogues of betaine, were also investigated. As observed for betaine, methylglycine decreased DeltaAUC(0-240) to 44% of the control, whereas glycine showed no significant effect on DeltaAUC(0-240), indicating that methyl groups of betaine and dimethylglycine were necessary to suppress plasma homocysteine elevation. These results suggest that betaine contributes to the suppression of plasma homocysteine elevation by promoting homocysteine metabolism, and seems to work as a methyl donor. (C) 2004 Elsevier Inc. All rights reserved., Nov. 2004, 15, 11, 666, 671, Scientific journal, 10.1016/j.jnutbio.2004.05.004
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Not Refereed, Reproductive Medicine and Biology, John Wiley and Sons Ltd, Current problems of food intake in young women in Japan: Their influence on female reproductive function, Tomoko Fujiwara; Rieko Nakata, Accumulating evidence suggests that food customs are associated with quality of life in women of the reproductive age. In Japan, dietary limitation for cosmetic purposes, skipping food intake, intake of processed foods and the shift from Japanese to Westernized style food have increased among young women. These changes in food habits can cause inadequate intake of calories, micronutrients, unsaturated fat, phytestrogens and fiber as well as increasing environmental toxins. Furthermore, these food habits increase risk as a result of intake of food additives, anti-oxidants, processing agents and sweeteners, which have been demonstrated to be harmful to human health. These factors are speculated to not only influence the present lifestyle, but also to induce gynecologic disorders such as dysmenorrhea and irregular menstruation. The adverse effects of these dietary habits on pregnancy outcome and carcinogenesis of breast and ovarian cancers have also been demonstrated. In addition, latent development of organic diseases such as endometriosis, which are accompanied by dysmenorrhea, is a concern under the current nutritional environment in young women. Thus, it is an urgent issue to evaluate the present situation of eating habits in young Japanese women and estimate the influence of these habits on the quality of life including reproductive functions., 2004, 3, 3, 107, 114, 10.1111/j.1447-0578.2004.00063.x
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Not Refereed, Current Topics in Biochemical Research, Regulation on reproductive functions by membrane-bound cell surface peptidases., NAKATA Rieko; Fujiwara H; Nakata R; Fujiwara T; Ueda M; Maeda M, 2004, 6, 1, 13

Not Refereed, 2002, 48, 1, 57, 76

Not Refereed, BIOCHEMICAL PHARMACOLOGY, PERGAMON-ELSEVIER SCIENCE LTD, Change in caspase-3-like protease in the liver and plasma during rat liver regeneration following partial hepatectomy, S Hayami; M Yaita; Y Ogiri; F Sun; R Nakata; S Kojo, Recent studies have shown that many factors orchestrate liver regeneration after a two-thirds partial hepatectomy (PH). However, the termination mechanism in liver regeneration has not been thoroughly studied. In this paper, we report that the activity of liver caspase-3-like protease, which is specifically activated in apoptosis, increases 18, 36, and 48 hr after PH during maximal hepatocyte proliferative activity. This is the first study that shows the activation of an apoptosis-executing enzyme during physiological liver regeneration. These results suggest that apoptosis is induced in each surge of DNA synthesis as the termination mechanism. When phenoxybenzamine, an alpha -blocker that has been reported to inhibit DNA synthesis during liver regeneration, was injected 8 hr after PH, the caspase-3-like activity in the liver peaked at 15 hr after PH and the enzyme activity also increased in plasma at 18 and 24 hr after PH in sharp contrast to the case of normal regeneration. These results indicate that extensive apoptosis is caused by phenoxybenzamine and that the secondary necrosis of apoptotic cells results in the increase of caspase-3-like protease activity in the plasma. BIOCHEM PHARMACOL 60;12:1883-1886, 2000. (C) 2000 Elsevier Science Inc., Dec. 2000, 60, 12, 1883, 1886, Scientific journal, 10.1016/S0006-2952(00)00508-6
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Not Refereed, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, CENTER ACADEMIC PUBL JAPAN, Determination of folate derivatives in rat tissues during folate deficiency, R Nakata, A method for the sensitive and specific determination of folate derivatives was developed. The method involves hydrolysis by gamma -glutamyl hydrolase and high-performance liquid chromatography with electrochemical detection. The method was applied to measure the change in the level of folate derivatives in the liver, kidney, spleen and brain of rats during folate deficiency. 5,6,7,8-Tetrahydrofolic acid was the major folate derivative in the liver, kidney, spleen and brain. Total concentration of folate derivatives decreased from the second week of folate deficiency in the liver, kidney spleen and brain followed by anemia, which appeared at the fifth week. The level of 5,6,7,8-tetrahydrofolic acid in the brain did not change during folate deficiency, but it significantly decreased in the liver, kidney and spleen., Oct. 2000, 46, 5, 215, 221, Scientific journal, 10.3177/jnsv.46.215
DOI URL

Not Refereed, JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOC, Facile degradation of apolipoprotein B by radical reactions and the presence of cleaved proteins in serum, K Tanaka; H Iguchi; S Taketani; R Nakata; S Tokumaru; T Sugimoto; S Kojo, A facile cleavage of peptide bonds of apolipoprotein B (apoB) by radical reaction is reported, When human LDL was subjected to oxidative damage using Cu2+, extensive degradation of apoB was observed based on immunoblotting, The degradation of apoB was inhibited by radical scavengers (beta-mercaptoethanol, butylated hydroxytoluene, and probucol) and promoted by a radical initiator [2,2'-azobis(2-amidinopropane)dihydrochloride], When human serum was treated with Cu2+, a similar cleavage pattern of apoB was observed. The cleaved apoB proteins were also detected in normal serum on the basis of immunoblots, These results suggest that apoB is highly reactive toward radicals in vitro and in vivo, with reaction resulting in the cleavage of peptide bonds., Jan. 1999, 125, 1, 173, 176, Scientific journal

Not Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Change ire the level of vitamin C and lipid peroxidation in tissues of the inherently scorbutic rat during ascorbate deficiency, S Tokumaru; S Takeshita; R Nakata; Tsukamoto, I; S Kojo, To investigate an accurate profile of vitamin C deficiency, ascorbate deficiency was caused in the inherently scorbutic rat [Osteogenic Disorder Shionogi (ODS)], and changes in the level of the vitamin in 12 tissues of the animals (plasma, liver, stomach, small and large intestines, lung, heart, kidney, adrenal gland, spleen, muscle, and brain) were followed based on the specific method (Kishida et al. Anal. Chem. 1992, 64, 1505-1507). The level of ascorbate in plasma decreased most rapidly, and the rate of decline of the vitamin was the slowest in the brain among the 12 tissues. Based on the kinetic profile of ascorbate decay, these tissues were classified into four groups. After 25 days of ascorbate deficiency, indicators of oxidative stress changed significantly compared with the control group. The indices included increased lipid hydroperoxide level determined by the specific method (Tokumaru et al. Anal. Chim. Acta 1995, 307, 97-102) in the brain, elevated thiobarbituric acid-reactive substances (TBARS) and glutathione peroxidase activity in the heart, and the fall of glutathione in plasma and the liver., Sep. 1996, 44, 9, 2748, 2753, Scientific journal, 10.1021/jf960184u
DOI URL

Not Refereed, Journal of home economics of Japan, Effects of Irregular Feeding under an 80% Energy Restricted Condition on the Growth and the Daily Energy Metabolism in Rats, Toyohara Masako; Ando Mami; Nakata Rieko; Miyoshi Masamitsu, Effects of irregular feeding on a 2 days cycle under an 80% energy restriction were examined on the growth rate, biochemical composition of liver and blood, and daily energy metabolism in 6-12 weeks old rats. The rats were divided into four groups which were fed an experimental diet for 38 days ad libitum (A) every day, 80% diet of group A every day (R80), 100 and 60% diets of group A on alternate days (R100-60), and 120 and 40% diets of group A on alternate days (R120-40), respectively. Body weight gain was significantly lower in group R120-40 than in the other groups. The weights of internal organs were lower in all the restricted groups than in group A. However, their weight ratios to whole body weight were not significantly different among all the groups. Differences in such daily energy metabolism as total energy expenditure per kg body weight were not significant between group A and group R80, which suggested that the rats in group R80 attained metabolic adaptation to the feeding conditions by diminishing their body size. On the other hand, the rats in group R120-40 changed total energy expenditure (TEE) and minimal energy expenditure (MEE) according to the amount of food ingested on alternate days. They maintained MEE on 120% diet days at a level comparable to that of the rats in group R80 and adapted the present irregular feeding conditions by decreasing total activity (TA). However, the decrease in TA brought about by irregular feeding over a long period of time is considered to be undesirable for health., 1995, 46, 9, 833, 840, 10.11428/jhej1987.46.833

Not Refereed, BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, ACADEMIC PRESS AUST, REGULATION OF THYMIDYLATE SYNTHASE IN REGENERATING RAT-LIVER AFTER PARTIAL-HEPATECTOMY, M WAKABAYASHI; R NAKATA; TSUKAMOTO, I, Sep. 1994, 34, 2, 345, 350, Scientific journal

Not Refereed, 1994, 68, 8, 445, 449, 10.20632/vso.68.8_445
DOI URL

Not Refereed, BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, ACADEMIC PRESS AUST, EFFECT OF AGING ON RAT-LIVER REGENERATION AFTER PARTIAL-HEPATECTOMY, TSUKAMOTO, I; R NAKATA; S KOJO, Jul. 1993, 30, 4, 773, 778, Scientific journal

Not Refereed, Eiyo To Shokuryo, Japan Society of Nutrition and Food Science, Circadian Changes in Physical and Physiological Capacity for Exercise., KATO Hideo; ISHIKURA Minako; OKA Michiko; MUNEMASA Kazumi; OKAMOTO Tsunemi; NAKATA Rieko; TAGUCHI Tomoko, Since various functions of the body have their own biorhythms adapted to the living environment, it is considered that the reactivity of physical locomotion may vary depending on the time of day when exercise is performed. In this study, in order to assess differences in locomotive capacity according to the time of day, 18 male high school baseball players were tested for physical strength in the morning (8: 00) and evening (18: 00). Furthermore, the time differences in endocrine and metabolic responses after physical exercise were assessed according to biorhythms. (1) Physical capacity changed according to the time of evaluation, and was greater in the evening than in the morning. (2) Among physiological functions that determine the development of physical strength, thyroid-stimulating hormone (TSH) and growth hormone glasma levels were higher after evening exercise than after morning exercise. (3) The increase in urinary excretion of 3-methylhistidine (degradation index of muscle protein) after exercise was smaller in the evening than in the morning. These findings suggest that evening exercise promotes the development of physical strength more than morning exercise., 1993, 46, 1, 33, 38, 10.4327/jsnfs.46.33
DOI URL

Not Refereed, BIOCHEMISTRY INTERNATIONAL, ACADEMIC PRESS AUST, EFFECT OF ENDOTOXIN ON RAT-LIVER REGENERATION AFTER PARTIAL-HEPATECTOMY, TSUKAMOTO, I; R NAKATA; S KOJO, Sep. 1992, 27, 6, 1047, 1050, Scientific journal

Not Refereed, BIOCHIMICA ET BIOPHYSICA ACTA, ELSEVIER SCIENCE BV, A NEW IMMUNOBLOTTING ASSAY FOR THYMIDYLATE SYNTHETASE AND ITS APPLICATION TO THE REGULATION OF ENZYME-ACTIVITY IN REGENERATING RAT-LIVER, TSUKAMOTO, I; R NAKATA; M MIYOSHI; S TAKETANI; S KOJO, Feb. 1988, 964, 2, 254, 259, Scientific journal

Not Refereed, BIOCHIMICA ET BIOPHYSICA ACTA, ELSEVIER SCIENCE BV, PURIFICATION AND CHARACTERIZATION OF THYMIDYLATE SYNTHETASE FROM RAT REGENERATING LIVER, R NAKATA; TSUKAMOTO, I; M MIYOSHI; S KOJO, May 1987, 924, 2, 297, 302, Scientific journal

Not Refereed, CLINICAL SCIENCE, PORTLAND PRESS, EFFECT OF THYROPARATHYROIDECTOMY ON THE ACTIVITIES OF THYMIDYLATE SYNTHETASE AND THYMIDINE KINASE DURING LIVER-REGENERATION AFTER PARTIAL-HEPATECTOMY, R NAKATA; TSUKAMOTO, I; M MIYOSHI; S KOJO, Apr. 1987, 72, 4, 455, 461, Scientific journal

Not Refereed, BIOCHEMICAL PHARMACOLOGY, PERGAMON-ELSEVIER SCIENCE LTD, LIVER-REGENERATION IN STREPTOZOTOCIN-DIABETIC RATS, R NAKATA; TSUKAMOTO, I; M MIYOSHI; S KOJO, Mar. 1986, 35, 5, 865, 867, 10.1016/0006-2952(86)90256-X
DOI URL

Not Refereed, EUROPEAN JOURNAL OF PHARMACOLOGY, ELSEVIER SCIENCE BV, ALPHA-ADRENERGIC REGULATION OF THE ACTIVITY OF THYMIDYLATE SYNTHETASE AND THYMIDINE KINASE DURING LIVER-REGENERATION AFTER PARTIAL-HEPATECTOMY, R NAKATA; TSUKAMOTO, I; M NANME; S MAKINO; M MIYOSHI; S KOJO, 1985, 114, 3, 355, 360, Scientific journal, 10.1016/0014-2999(85)90380-2
DOI URL

Not Refereed, BIOCHEMICAL PHARMACOLOGY, PERGAMON-ELSEVIER SCIENCE LTD, LIVER-REGENERATION AFTER CARBON-TETRACHLORIDE INTOXICATION IN THE RAT, R NAKATA; TSUKAMOTO, I; M MIYOSHI; S KOJO, 1985, 34, 4, 586, 588, 10.1016/0006-2952(85)90195-9
DOI URL

Refereed, 2025

Refereed, 2024, 16, 3574

Refereed

Refereed, 2023, 42, 111940

MISC

Not Refereed, 日本栄養・食糧学会大会講演要旨集, 米麹抽出物が脂質代謝に与える効果に関する研究, 高橋春弥; CHI Hsin‐Yi; 毛利晋輔; 鎌苅浩介; 中田啓司; 一條範好; 中田理恵子; 井上裕康; 後藤剛; 後藤剛; 河田照雄; 河田照雄, 27 Apr. 2018, 72nd, 224
URL

Not Refereed, 日本農芸化学会大会講演要旨集(Web), 麹抽出物の脂質代謝改善作用に関する研究, 高橋春弥; CHI Hsin‐Yi; 毛利晋輔; 鎌苅浩介; 中田啓司; 一條範好; 中田理恵子; 井上裕康; 後藤剛; 後藤剛; 河田照雄; 河田照雄, 05 Mar. 2017, 2017, ROMBUNNO.2A08p08 (WEB ONLY)
URL

Not Refereed, 2017, 59, 54, 56

Not Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Rice Koji Extract Enhances Lipid Metabolism through Peroxisome Proliferator-Activated Receptor Alpha (PPAR alpha) Activation in Mouse Liver (vol 64, pg 8848, 2016), Haruya Takahashi; Hsin-Yi Chi; Shinsuke Mohri; Kosuke Kamakari; Keiji Nakata; Noriyoshi Ichijo; Rieko Nakata; Hiroyasu Inoue; Tsuyoshi Goto; Teruo Kawada, Jan. 2017, 65, 1, 251, 251, Others, 10.1021/acs.jafc.6b05717
DOI URL

Refereed, 日本栄養・食糧学会近畿支部大会および公開シンポジウム講演抄録集, 脂質代謝亢進時における代謝変動の網羅的解析及び変動代謝物の機能解析, 高橋春弥; 後藤剛; 山崎陽太; 鎌苅浩介; 平田茉莉子; 柴田大輔; 中田理恵子; 井上裕康; 高橋信之; 高橋信之; 河田照雄, 14 Sep. 2016, 55th, 44
URL

Not Refereed, 日本生化学会大会プログラム・講演要旨集, (公社)日本生化学会, 腸内細菌の長鎖不飽和脂肪酸代謝物によるGPR120活性化の検討, 本郷翔子; 森本育美; 山上小百合; 古田美咲; 滝澤祥恵; 井上飛鳥; 青木淳賢; 東山繁樹; 吉田守克; 宮里幹也; 岸野重信; 小川順; 中田理恵子; 井上裕康, Sep. 2016, 89回, [2P, 106]

Study of the Molecular Targets of Metabolic Improvement Caused by Soy Protein Intake, Jun. 2016, 18, 36, 12, 16

Refereed, 日本栄養・食糧学会大会講演要旨集, フィトールおよびその代謝産物のPPARα活性化能が肝臓および脂肪組織における脂質代謝に及ぼす影響, 眞田康平; 安芝英; 後藤剛; 高橋春弥; 永井宏幸; 金英一; 中田理恵子; 井上裕康; 高橋信之; 高橋信之; 河田照雄, 25 Apr. 2016, 70th, 232
URL

Not Refereed, 日本栄養・食糧学会大会講演要旨集, (公社)日本栄養・食糧学会, 活動期に対応したラットの摂食行動はカロリー制限による卵巣機能抑制の新たな制御因子か?, 藤原智子; 山岸郁乃; 井上裕康; 中田理恵子; 藤原浩, Apr. 2016, 70回, 274, 274

Not Refereed, 2016, 58, 53, 54

Not Refereed, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, (公社)日本生化学会, GPR120活性化を指標とする新規食品機能評価系の検討, 森本育美; 滝澤祥恵; 本郷翔子; 井上飛鳥; 青木淳賢; 東山繁樹; 吉田守克; 宮里幹也; 中田理恵子; 井上裕康, Dec. 2015, 88回・38回, [2P1232], [2P1232]

Refereed, 肥満研究, メタボロミクスを用いたPPARα活性化時の変動代謝物に関する研究, 高橋春弥; 後藤剛; 山崎陽太; 鎌苅浩介; 平田茉莉子; 鈴木秀幸; 柴田大輔; 中田理恵子; 井上裕康; 高橋信之; 河田照雄, 08 Sep. 2015, 21, Supplement, 208
URL

25 Apr. 2015, 89, 4, 219, 219

Not Refereed, ビタミン, (公社)日本ビタミン学会, TGFα切断アッセイを用いたω-3系脂肪酸によるGPR120活性化, 森本育美; 滝澤祥恵; 本郷翔子; 井上飛鳥; 青木淳賢; 東山繁樹; 吉田守克; 宮里幹也; 中田理恵子; 井上裕康, Apr. 2015, 89, 4, 223, 223

Not Refereed, 2015, 57, 153, 155

25 Apr. 2014, 88, 4, 224, 224

25 Apr. 2014, 88, 4, 231, 231

Not Refereed, 2014, 56

25 Apr. 2013, 87, 4, 206, 206

25 Apr. 2013, 87, 4, 224, 224

Not Refereed, 2013, 55, 94, 96

Not Refereed, 2013

25 Apr. 2012, 86, 4, 283, 283

25 Apr. 2012, 86, 4, 280, 280

Not Refereed, 2012, 54, 252, 255

Not Refereed, 2012, 54, 12, 1, 6

Not Refereed, 2012, 1, 33, 37

Not Refereed, CHEMISTRY AND PHYSICS OF LIPIDS, ELSEVIER IRELAND LTD, A possible function of resveratrol and essential oil-derived chemicals for prevention of life-style related diseases targeted to COX-2 and PPAR, Hiroyasu Inoue; Yoshie Takizawa; Ayako Takai; Chisako Takashiba; Satomi Koeji; Chiharu Iwasa; Rieko Nakata, Aug. 2011, 164, S29, S29, Summary international conference, 10.1016/j.chemphyslip.2011.05.095
DOI URL

25 Apr. 2011, 85, 4, 225, 225

25 Apr. 2011, 85, 4, 251, 251

Not Refereed, 2011, 69, 41, 43

Not Refereed, VITAMINS, THE VITAMIN SOCIETY OF JAPAN, Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ in vivo and in vitro, Tsukamoto Tomoko; Nakata Rieko; Tamura Emi; Kosuge Yukiko; Kariya Aya; Katsukawa Michiko; Mishima Satoshi; Itoh Tetsuro; Iinuma Munekazu; Akao Yukihiro; Nozawa Yoshinori; Arai Yuji; Namura Shobu; Inoue Hiroyasu, 2011, 85, 2, 70, 72, 10.20632/vso.85.2_70
DOI URL

Not Refereed, 2011, 53

Not Refereed, 2011, 53, 144, 146

Not Refereed, 2011, 20, 6, 9

25 Apr. 2010, 84, 4, 179, 179

25 Apr. 2010, 84, 4, 203, 203

Not Refereed, 2010, 52, 199, 201

Not Refereed, 2010, 84, 255, 256

25 Apr. 2009, 83, 4, 175, 175

25 Apr. 2009, 83, 4, 190, 190

Not Refereed, CHEMICAL SENSES, OXFORD UNIV PRESS, Functional Expression of Miraculin, a Taste-Modifying Protein in Arabidopsis thaliana and Escherichia coli, Makiko Satoh; Tomomi Matsuyama; Rieko Nakata; Takashi Aoyama; Hiroyasu Inoue, Feb. 2009, 34, 2, J14, J14, Summary international conference

Not Refereed, ANNALS OF NUTRITION AND METABOLISM, KARGER, RESVERATROL ACTIVATES NUCLEAR RECEPTOR PPARS IN VITRO AND IN VIVO, Rieko Nakata; Emi Tamura; Yukiko Kosuge; Aya Kariya; Michiko Katsukawa; Hiroyasu Inoue, 2009, 55, 160, 160, Summary international conference

Not Refereed, 小児がん, (NPO)日本小児がん学会, High-dose MTX投与後の葉酸代謝物質とセラミド、ビタミンEの血中動態, 久保田優; 市育代; 中田理恵子; 足立壮一; 渡邊健一郎; 松原央; 中畑龍俊; 樋口万緑; 吉岡章, Nov. 2008, 45, プログラム・総会号, 192, 192

25 Apr. 2008, 82, 4, 282, 282

25 Apr. 2008, 82, 4, 299, 299

Not Refereed, 2008, 12, 4

Not Refereed, 2008, 50, 304, 307

Not Refereed, 2008, 50, 300, 303

25 Apr. 2007, 81, 4, 149, 149

25 Apr. 2007, 81, 4, 157, 157

08 Jun. 2006, 48, 131, 134

25 Apr. 2006, 80, 4, 217, 217

Not Refereed, 2006, 48, 141, 143

Not Refereed, 2004, 17, 35, 47

Not Refereed, 2003, 56, 11, 1260

25 Apr. 1997, 71, 4, 186, 186

Not Refereed, Journal of Home Economics of Japan, Effects of Irregular Feeding under an 80% Energy Restricted Condition on the Growth and the Daily Energy Metabolism in Rats, NAKATA Rieko, Effects of irregular feeding on a 2 days cycle under an 80% energy restriction were examined on the growth rate, biochemical composition of liver and blood, and daily energy metabolism in 6-12 weeks old rats. The rats were divided into four groups which were fed an experimental diet for 38 days ad libitum (A) every day, 80% diet of group A every day (R80), 100 and 60% diets of group A on alternate days (R100-60), and 120 and 40% diets of group A on alternate days (R120-40), respectively. Body weight gain was significantly lower in group R120-40 than in the other groups. The weights of internal organs were lower in all the restricted groups than in group A. However, their weight ratios to whole body weight were not significantly different among all the groups. Differences in such daily energy metabolism as total energy expenditure per kg body weight were not significant between group A and group R80, which suggested that the rats in group R80 attained metabolic adaptation to the feeding conditions by diminishing their body size. On the other hand, the rats in group R120-40 changed total energy expenditure (TEE) and minimal energy expenditure (MEE) according to the amount of food ingested on alternate days. They maintained MEE on 120% diet days at a level comparable to that of the rats in group R80 and adapted the present irregular feeding conditions by decreasing total activity (TA). However, the decrease in TA brought about by irregular feeding over a long period of time is considered to be undesirable for health., 1995, 46, 9, 833, 840, 10.11428/jhej1987.46.833

Not Refereed, Vitamins, The Vitamin Society of Japan, Effects of Folic Acid on Rat Liver Regeneration After Partial Hepatectomy, NAKATA Rieko, Effects of folic acid on rat liver regeneration were evaluated by the activities of thymidylate synthase and thymidine kinase, which are rate-limiting enzymes of DNA synthesis in liver regeneration, and the contents of DNA, RNA and protein, and liver weight. By injection of folic acid immediately after partial (70%) hepatectomy, thymidine kinase activity was inhibited in the 24 h-regenerating liver, whereas thymidylate synthase activity increased in the 48 and 72 h-regenerating liver, The DNA and RNA contents and liver weight were also reduced in the 72 h-regenerating liver of folate-injected rats. These results suggest the stimulatory effect of folic acid on the induction of thymidylate synthase and the inhibitory effect on rat liver regeneration., 1994, 68, 8, 445, 449, 10.20632/vso.68.8_445

Not Refereed, Biochemistry and Molecular Biology International, Effect of ageing on rat liver regeneration after partial hepatectomy, I. Tsukamoto; R. Nakata; S. Kojo, The effect of ageing on liver regeneration after two-thirds partial hepatectomy was evaluated using rats of 6 and 60 weeks of age. The induction of thymidylate synthase and thymidine kinase, which are rate-determining enzymes of DNA synthesis in liver regeneration, delayed by 24 h and the maximal activities were significantly lower in old rats. Effects of aging on liver weight and synthesis of DNA, RNA and protein were discussed quantitatively in terms of the relative restoration yield deduced from the comparison with the young animals., 1993, 30, 4, 773, 778

Not Refereed, 1990, 43, 12, 2076, 2083

Not Refereed, 1990, 26, 101, 107

Not Refereed, Journal of Hiroshima Medical Association, Circadian changes in the development of physical strength by exercise, NAKATA Rieko, 1990, 43, 12, 2076, 2083

Not Refereed, 1989, 25, 25, 1, 7

Books etc

2018, Not Refereed

2017, Not Refereed

2015, Not Refereed

2014, Not Refereed

2011, Not Refereed

2011, Not Refereed

2010, Not Refereed

2010, Not Refereed

2007, Not Refereed

2007, Not Refereed

2006, Not Refereed

2006, Not Refereed

2004, Not Refereed

2003, Not Refereed

Presentations

May 2020

May 2020

The 9th International Conference on Polyphenols and Health - ICPH2019（, The direct activation of PPARalpha by 4’-hydroxyl group of resveratrol and a feedforward regulation via cAMP, Nov. 2019

Sep. 2019

Sep. 2019

Jul. 2019

Jun. 2019

May 2019

May 2019

May 2019

Mar. 2019

Sep. 2018

Sep. 2018

Sep. 2018

Sep. 2018

May 2018

May 2018

May 2018

May 2018

May 2018

2012

2012

2012

2012

2012

2012

2012

2012

2012

2012

2012

2012

2012

2012

Keystone symposium -Aging and Diseases of Aging-, 4'hydroxy group of resveratrol plays a key role in PPARα activation, 2012

Keystone symposium -Aging and Diseases of Aging-, Resveratrol improves lipid metabolism and life span via PPARα., 2012

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

2011

XI Asian Congress of Nutrition, Resveratrol and its tetramer activate PPARs in vitro and in vivo., 2011

XI Asian Congress of Nutrition, A possible novel function of Asian herbs for prevention of lifestyle-related diseases targeted to COX-2 and PPARs., 2011

XI Asian Congress of Nutrition, Skipping breakfast adversely affects reproductive function in post-adolescent female college students., 2011

52nd International Conference on the Bioscience of Lipids, A possible function of resveratrol and essential oil-derived chemicals for prevention of lifestyle-related diseases targeted to COX-2 and PPAR., 2011

2011 International Conference on Food Factors, Activation of PPARs by resveratrol in vitro and in vivo., 2011

2011 International Conference on Food Factors, A possible function of resveratrol and essential oil-derived chemicals for prevention of lifestyle-related diseases targeted COX-2 and PPAR., 2011

2010

2010

2010

2010

2010

2010

2010

2010

2010

2010

2010

2010

2010

2010

2010

2010

Keystone symposium (Bioactive Lipids: Biochemistry and Diseases), Resveratrol activates PPARs in vitro and in vivo., 2010

Keystone symposium (Bioactive Lipids: Biochemistry and Diseases), Evaluation of essential oils by activation of PPARs and suppression of COX-2 expression, 2010

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

2009

4th International Conference on Phospholipase A2 and Lipid mediators, Change in gene expression of lipid metabolic enzyme in liver and adipose tissue of the folate-deficient rats., 2009

4th International Conference on Phospholipase A2 and Lipid mediators, Evaluation of essential oils by activation of PPARs and suppression of COX-2 expression., 2009

19th International Congress of Nutrition, Resveratrol activates nuclear receptor PPARs in vitro and in vivo., 2009

Bioactive Lipid Conference, Carvacrol and other components of essential oils activate PPARs and suppress COX-2 expression., 2009

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

2008

Poster presentation

Poster presentation

Poster presentation

Poster presentation

Poster presentation

Oral presentation

Poster presentation

Poster presentation

Works

2008 - 2009

2007 - 2008

2006 - 2007

2005 - 2006

2005 - 2006

Research Projects

基盤研究(B), 01 Apr. 2024 - 31 Mar. 2027, 24K00362, 異常な空腹ストレスによる摂食制御機構の破綻－思春期の摂食障害と次世代への影響－, 藤原智子; 三枝理博; 大黒多希子; 安藤仁; 細野隆; 小野政徳; 中田理恵子; 藤原浩, 日本学術振興会, 科学研究費助成事業, 京都ノートルダム女子大学, 18590000, 14300000, 4290000, kaken
対象リソースIRI

基盤研究(C), 01 Apr. 2022 - 31 Mar. 2025, 22K02181, 健康長寿社会の実現を促す葉酸の機能解析と食生活への応用, 中田理恵子, 日本学術振興会, 科学研究費助成事業, 奈良女子大学, 4160000, 3200000, 960000, kaken
対象リソースIRI

挑戦的研究(開拓), 09 Jul. 2021 - 31 Mar. 2025, 21K18297, 思春期の欠食とダイエットで性差をもって“リプログラミング”される食欲と代謝効率, 藤原智子; 三枝理博; 藤原浩; 大黒多希子; 堀家慎一; 安藤仁; 中田理恵子; 程肇; 水本泰成, 日本学術振興会, 科学研究費助成事業, 京都ノートルダム女子大学, 25740000, 19800000, 5940000, kaken
対象リソースIRI

2018 - Mar. 2021, Coinvestigator, 概日時計の乱れが誘発する若年女性の生殖機能障害の実態とその機序の解析－朝食欠食とダイエットに着目して－, 日本医療研究開発機構（AMED）, 女性の健康の包括的支援実用化研究事業

2014 - Mar. 2019, Coinvestigator, 次世代機能性農林水産物・食品の開発－運動・身体機能維持を促す次世代機能性食品の創製－, 内閣府, 戦略的イノベーション創造プログラム

Grant-in-Aid for Scientific Research (C), 2009 - 2011, 21500758, Development of new fermented food includingγ-amino butyric acid using various scallop, ISOBE Yuka; NAKATA Rieko; HIRASHIMA Madoka, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Mie University, 4420000, 3400000, 1020000, The new fermented food made from shellfish includingγ-amino butyric acid(GABA) was developed using the strain isolated from various food in our laboratory. The optimal condition for producing GABA was clarified. Manufacturing conditions of fermented food, such as composition of materials and sanitary pretreatment, were established. It was clarified that production of fermented food used by Japanese scallop, noble scallop(hiogi-gai), and Japanese pearl oyster was possible. The manufactured fermented food had higher GABA content than some commercial products containing GABA., kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (B), 2007 - 2009, 19300250, Elucidation of mechanism on "French Paradox" toward prevention of lifestyle-related diseases, INOUE Hiroyasu; NAKATA Rieko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nara Women's University, 19630000, 15100000, 4530000, Dairy oral intake of resveratrol, a phytoalexin contained in red wine, reduces plasma triglyceride concentration, and upregulates hepatic expression of PPAR-α responsive genes in the wild-type but not PPARα knockout mice. Moreover, long-term intake of 0.02% resveratrol with high fat diet prevents body weight gain observed after 46 weeks and accumulation of abdominal white adipose tissue, and improves survival of mice. These findings will account for a novel mechanism that may afford beneficial effects of preventing lifestyle-related diseases.
Several natural chemicals such as resveratrol have been identified as suppressors of COX-2 expression and activators of PPARs. These two properties targeted to COX-2 and PPARs will be useful in evaluating functional food components against lifestyle-related diseases. In this context, we evaluated oils from various plants in cell-based transfection assays, and found that six essential oils from thyme, rose, clove, fennel, eucalyptus, and bergamot, have these properties. From thyme oil, we identified carvacrol as a major component of the activator of PPARα and γ and suppressor of COX-2 expression, and PPARγ-dependent suppression of COX-2 promoter activity was observed in response to carvacrol treatment. These results will be important in understanding the anti-lifestyle-related disease properties of essential oils., kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (C), 2007 - 2008, 19500707, Survey and experimental verification of adverse effects of dietary habits on reproductive functions in post-adolescence women, FUJIWARA Tomoko; NAKATA Rieko; FUJIWARA Hiroshi, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Ashiya College, 4550000, 3500000, 1050000, 性成熟過程にある女性の食生活は以後の母性を担う時期の女性のQOLに重要な影響を与えるという新しい着想から、悪影響を及ぼす可能性のある食生活習慣の因子を抽出することを目的として、18-20歳の女子学生を対象に食事習慣を含む基本的な生活習慣、体型認識やダイエット行動の実態、婦人科愁訴をふくむ身体症状についてのアンケート調査を平成19年度に引き続いて行った。また奈良女子大学の倫理委員会に諮り、承認を得た上でボランティア学生を募って3ヶ月に及ぶ食事調査を実施し、合わせて基礎体温や婦人科愁訴などのデータも収集した。食事内容についてはデジタルカメラで撮影した記録をもとに詳細な栄養分析を行い、朝食の欠食とともに生殖機能に障害をもたらす可能性のある食習慣について調査した。その結果、女子学生の食生活上の問題点として新たに、ファストフードやコンビニエンスストアで売られている加工食品を多用するといった他の有害な食習慣を有していることが示された。ファストフードや加工食品の摂取頻度の多いグループにおいても月経痛の程度が強いことが観察され、食習慣が将来の婦人科疾患の誘因になる可能性を支持する結果が示された。一方、朝食欠食についても月経周期の異常すなわち性機能に機能的に悪影響を及ぼす可能性が示されたが、さらにファストフードや加工食品の多用者に比べ朝食を欠食している学生に体調不良を訴える者が多いことが明らかとなった。以上の知見より一日の活動の最初の段階での飢餓状態を生じる朝食の欠食は女子学生のQOLに影響を及ぼすもっとも重要な因子のひとつである可能性が示唆された。本研究にて得られた結果は性成熟完成期女性に対してより効果的な教育プログラムの開発を進める上で意義ある知見といえる。, kaken
対象リソースIRI

萌芽研究, 2006 - 2007, 18650213, ミラクリン蛋白質による味覚変革機構の検討, 井上裕康; 中田理恵子, 日本学術振興会, 科学研究費助成事業, 奈良女子大学, 3300000, 3300000, ミラクリンは酸味を甘味に変換する蛋白質として知られている。しかし、ミラクリンがどのような分子機構によって、味覚変革を引き起こすかは不明である。一方、味覚受容体として、甘味を感知する受容体(T1R2/T1R3)、酸味を感知する受容体などが見出されている。これらの物質レベルでの知見から、ミラクリン蛋白質と甘味受容体、あるいは酸味受容体との相互作用が味覚変革の分子機構である可能性が高い。本研究では、味覚変革機構の解明を目標とし、まず味覚変革蛋白質ミラクリンを活性のある形で、大量に発現するシステムの構築を進めた。その結果、世界で初めて、大腸菌を用いた活性型ミラクリンの発現、精製に成功した(論文投稿準備中)。これは今まで、ミラクリンの味覚修飾作用には糖鎖が必要であるとする従来の定説を覆すものであるが、同時にミラクリンが活性を持つためには、ホモ二量体を形成することが必須であることを示している。なお糖鎖を持つシロイヌナズナを用いた発現系ではほぼ全てが二量体を形成するのに対し、糖鎖を持たない大腸菌を用いた発現系では、二量体を形成するものが少ないことがわかり、ミラクリンの糖鎖は二量体形成の安定性に寄与することが示唆された。一方、甘味受容体を培養細胞に発現する系の構築を行い、免疫学的に蛋白質の発現を確認した。今後は、受容体蛋白質と組換えミラクリンとの相互作用を検討していく予定である。なお、本研究の内容は平成19年度の「ひらめき、ときめきサイエンス」・「食のライフサイエンス」として採択され、高校生向けに研究の説明を行った。, kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (C), 2005 - 2006, 17500540, Study on estimation of biological function of folate -the importance of folate as prophylactic factor of arteriosclerosis-, NAKATA Rieko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nara Women's University, 3600000, 3600000, Folate plays an important role in the biosynthesis of nucleic acids and amino acids. It has been known that folate deficiency induces the inhibition of cell proliferation, and the increase of homocysteine, which one of the risk factor of vascular diseases. In this study, we investigated the effect on vascular function and the regulatory mechanism of homocysteine, which is increased during folate deficiency.
The folate-deficient rats received an amino acid-defined, and folate-deficient diet. The control rats were fed the same diet supplemented with 8 mg/kg diet. The rats were killed by collecting the blood at the 4, 6, 8 weeks, and the liver and vessel was excised.
The concentrations of folate derivatives in plasma and liver of folate-deficient rats were significantly decreased. On the other hand, the concentrations of homocysteine on plasma and liver during folate deficiency were significantly increased. In vessel, the content of TBARS was increased, and the contents of glutathione and vitamin C were decreased. In folate-deficient rats, the concentration of nitric oxide (NO) in plasma was significantly decreased, and the protein of endothelial NO synthase was decreased by detection of western blotting. These results suggest that the increase of homocysteine during folate deficiency induced oxidative stress in vessel following dysfunction, which NO synthesis is deceased.
Secondly, it was examined that the change in gene expression of homocysteine metabolic enzymes during folate deficiency by real time RT-PCR method. In the folate-deficient rats, the levels of mRNA were significantly decreased in 5,10-methylelnetetrabhydrofolate reductase (MTHFR), methionine synthase (MS) and cystathionine-β-synthase (CBS), which are involved in the metabolism of homocysteine, compared to the control rats. These results suggest that the increase of homocysteine during folate deficiency is regulated by gene expressions of MTHFR, MS and CBS., kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (B), 2003 - 2005, 15402001, Investigative Studies on Women's lives and their Living Environments in Xinjiang Uygur Autonomous Region in China, IWASAKI Masami; SETO Akiko; MIYASAKA Yasuko; KUBO Hiroko; NAKATA Rieko; HATTORI Noriko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nara Women's University, 12200000, 12200000, We visited Urumchi, Kashgar, Atush, Hami, Turfan and Hotan, from 2003 to 2005 and aimed to conduct investigations on actual conditions of Uygur women and their living environments. In our investigation, we principally conducted oral interviews and observation in house visits, and made photographic records.
In conducting investigations, we were divided into groups according to each one's specialty, such as families, genders, cloth-ing, dietary culture, and living environments. In order to comprehend the situation of the region, we invited the cooperation of teachers of secondary school and Xinjian University, instructors of museums and foreign students of Uygur people.
We report about the following contents of our investigation about which we have formed a viewpoint.
1)Children's education 2)Ordinary lives and Religious lives like Ramadan 3)Expression of costumes as Muslim 4)Traditional customs and their changes in Family relations and lives.
Main problems of school-education are that the high school attendance rate has declined and that students who are not able to go to school have been increased. In particular, among the poor farmers' households that account for 80% of the region, parents do not have higher expectations for their children, thus they prevent their children from going to school.
Their main agent of child-rearing is the mother. Mothers raise children by receiving sufficient support from relatives, especially their own mothers.
Uygur people maintain the culture of gender division of domestic labor. Particularly Muslims maintain a strong patriarchal system so that their educational expectations for daughters are lower compared to sons., kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (C), 2002 - 2003, 14580140, Study on estimation of biological function of folate -The importance of folate intake during pregnancy -, NAKATA Reiko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nara Women's University, 3600000, 3600000, Folate is essential for DNA synthesis and cell proliferation. Specially, the folate deficiency of the maternal body effects on the health of progeny during pregnancy and lactation, that DNA synthesis is activated. It is expected that folate deficiency induce the inhibition of cell proliferation, and result in cell death (apoptosis). In this study, it was examined apoptosis after folate deficiency in rats of the growth, by measuring the activity of caspase-3, which is specifically activated in apoptosis. Caspase-3 activities in bone narrow were significantly higher than the activities of corresponding control rats at the 4, 6 and 8 weeks after folate deficiency. Furthermore, positive signals for apoptosis by immuno-histochemical method (TUNEL method) were detected in bone marrow of folate-deficient rats. These results were shown that the increase of caspase-3 activity occurred in bone marrow during folate deficiency, and was followed by inducing apoptosis.
Secondly, it was examined that the effect of folate intake during pregnancy and lactation in rats on the health of progeny. The pregnant rats received folate-deficient diet, or low-folate diet, or control diet during pregnancy and lactation, In folate-deficient rats, the survival of progeny was only 30%, and the body weight gain was lower than the low-folate and control rats, The folate contents in liver of progeny were influenced by the folate intake of maternal body. It was followed that the increases of caspase-3 activity in liver, spleen and bone marrow were appeared, and resulted in apoptosis. Moreover, the increases of homocysteine concentration in liver and plasma, and TBARS in liver were observed in progeny, and they expected the promotion of oxidant stress, Therefore, these results were shown that the decrease of folate intake during pregnancy and lactation in rats largely damaged the health of progeny, and that the folate intake during pregnancy was important to the health promotion of progeny., kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (B), 2000 - 2002, 12572041, Comparative study on Japanese and Chinese women and their living environments -Comparative study conducted in Xinjiang Uygur Autonomous Region in China, and Japan-, IWASAKI Masami; MIYASAKA Yasuko; SETO Akiko; KATSUTA Keiko; NAKATA Rieko; KUBO Hiroko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nara Women's University, 14000000, 14000000, Xinjiang Uygur Autonomous Region in China lies just halfway between Europe and Japan. We aimed to conduct investigations on the actual conditions of Uygur women and their lives. Because the population of Uygur people is the largest among the forty-seven minor ethnic groups in the region. In conducting investigations, we were divided into groups according to each one's specialty, such as families, gender, clothing, dietary culture and living environments, and we decided to compare a city and its surrounding area (a rural community), tradition and changes, and South Xinjiang and North Xinjiang. In order to comprehend the situation of the region, we invited the cooperation of researchers in the Center for Research on Women at Xinjiang University as our counterparts.
In 2000, we visited Urumchi and Turfan, and investigated the roles of the female staff of educational organizations, differences in the lives among typical ethnic groups, the labor of women who engaged in traditional industries, their tasks at home. In 2001, we visited Kashgar and Hotan, recorded the conditions of deeply religious lives connected with Islamic culture, and conducted a study on them. In 2002, in order to compare lives in South Xinjiang and North Xinjiang, we visited Yining, adjacent to Russia (the former Soviet Union) and investigated mostly farmers' lives. As a result, we witnessed remarkable affluence due to trade with Russia and other regions, and westernized freedom for various aspects, of then-lives, such as their home interiors and preferences for doming, unlike South Xinjiang. As the results of our comparison between the city and its surrounding area, and South Xinjiang and North Xinjiang, it was learned that close family relations common among Uygur people continued, but religious characteristics changed drastically in some regions. There were also problems, such as difficulty in the coexistence of international aspects with ethnical aspects., kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (C), 2000 - 2001, 12680131, Study on estimation of biological funciton of folate, NAKATA Rieko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nara Women's University, 3600000, 3600000, We investigated change of homocysteine concentrations in rat tissues, methyl group, metabolism, peroxidant and antioxidants during folate deficiency, for the purpose of esitimation of biological function of folate. Homocysteine concentrations of plasma, liver and heart of folate deficient rats were significantly higher than those of control rats. Thereforem these of kidney and brain did not differ significantly between folate deficiency group and control group. S-adenosylrnethionine(SAM), S-adenosylhomocysteine(SAH) hydrolase activity and L-methionine-S-adenosyl(MA) transferase activity in liver of rats fed folate deficient diet were significant higher, SAH and SAM/SAH ratio were lower than in liver of control rats. But in brain, these values did not differ among two groups. Thiobarbituric acid-reactive substances in liver, heart and kidney of folate deficient rats were significantly higher than the controls after 6-weeks feeding period, we measured antioxidants during folate deficiency. Vitamin C concentrations of liver, heart and plasma were significantly lower than the contols. Vitamin E of liver and plasma, and glutathione of liver in folate deficient rats were significantly lower than those in control rats. Glutathione peroxidase activity was not significantly altered. It was shown that methyl group methbolism was altered in liver, but not in brain, during folate deficiency. Further, these data demonstrate promotion of oxidative stress in liver, kidney and heart of folate deficient rats. However, folate deficient did not promote oxidative stress in brain., kaken
対象リソースIRI

奨励研究(A), 1998 - 1999, 10780081, 特異的定量法に基づく葉酸欠乏症の発症機序の解明, 中田理恵子, 日本学術振興会, 科学研究費助成事業, 奈良女子大学, 1900000, 1900000, 申請者は、葉酸欠乏症の発症機序、さらには生体での葉酸の機能を、化学的に正確な量を基礎として解明するための研究を推進している。本年度は、葉酸代謝とビタミンB_<12>との関係を検討した。ビタミンB_<12>は葉酸と同様に、DNA合成や細胞増殖にとって必須のビタミンであり、その欠乏によって葉酸代謝に何らかの変化をひき起こすと考えられているが、物質レベルで相互作用の検討は十分になされていない。そこで、生体内での葉酸とビタミンB_<12>の関係を明らかにするため、葉酸欠乏(F群)、ビタミンB_<12>欠乏(B群)、葉酸とビタミンB_<12>欠乏(BF群)ラットを各々6週間飼育して、その欠乏過程における葉酸誘導体量、ビタミンB_<12>量、葉酸代謝に関連する物質を定量した。肝臓中の葉酸誘導体は、還元条件下で肝臓より抽出した後、HPLCにて分離し、電気化学的に検出する方法により特異的に定量した。F群とBF群では、葉酸誘導体量が2週目より有意に減少した。また、B群においても、葉酸を十分摂取しているにもかかわらず、4週目から葉酸誘導体量の有意な減少がみられた。一方、肝臓中のビタミンB_<12>量は、B群、BF群において4週目より大きく減少したが、F群では減少はみられなかった。以上のように、ビタミンB_<12>の欠乏は葉酸誘導体量の減少をひき起こすが、葉酸の欠乏はビタミンB_<12>量に変化を与えないことが明らかになった。さらに、葉酸を基質とし、ビタミンB_<12>を補酵素として生体内で働くメチオニンシンターゼ活性を測定した結果、補酵素であるビタミンB_<12>の欠乏によって、B群とBF群において2週目より有意に減少した。また、肝臓中のヌクレオチド量は、F群、BF群において有意な変化がみられたが、B群では変化がみられなかった。F群、BF群においては、ヌクレオチド量のインバランスを反映して、肝臓中のDNA、RNA量の減少がみられた。, kaken
対象リソースIRI

Grant-in-Aid for Scientific Research (C), 1997 - 1999, 09680060, Survey of fibrynolytic enzymes in food materials and examination of their effects in vivo, TOYOHARA Masako; NAKATA Reiko; TOYOHARA Haruhiko; MURATA Michiyo, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, KACHO JUNIOR COLLEGE, 2600000, 2600000, The purpose of the present study was to search fibrinolytic activities among various food materials by using the fibrinolytic assay and fibrinogen zymography originally devised by the authors. The author also made attempts to examine the intake of the active enzyme in the blood of animal by oral administration and the reduction of the activity during curing.
Among more than 200 materials examined, the author successfully detected the strong activities in mushrooms in addition to natto as reported. Judged from the inhibitory effect by 1,10-phenanthroline, most of the activities from mushrooms were supposed to be derived from metalloproteinases. The most heat stable activity was found in edible mushroom basidiomycete (Grifola frondosa), which remained after short period heating at 90℃. Saute was shown to be the most suitable cooking to reserve the activity.
Purification of the enzyme by conventional ion exchange chromatographies from the fruiting body of basidiomycete was hampered possibly because the enzyme formed a complex with glycoproteins with various isoelectric points. By gel permeation chromatographies, the enzyme molecule was eluted as a relatively low molecular weight molecule (approximately 20kDa). The intake of the enzyme into blood by oral administration was examined by using antibodies raised against whole extract of basidiomycete, but significant intake was not immunologically detected. The cDNA possibly encoding the enzyme was cloned by PCR method using primers designed form the report determining the amino acid sequence of the enzyme (J. Biol. Chem., 272, 30032-30039(1999)). The successful cloning of the enzyme would lead the bacterial production of the large amount of the enzyme as recombinant proteins., kaken
対象リソースIRI

奨励研究(A), 1996 - 1996, 08780012, 特異的定量法に基づいた葉酸所要量の評価に関する研究, 中田理恵子, 日本学術振興会, 科学研究費助成事業, 奈良女子大学, 1000000, 1000000, 水溶性ビタミンである葉酸は、プリン,ピリミジン,アミノ酸,タンパク質など、生体の非常に重要な物質の合成に関与しており、生命の維持に基本的でかつ非常に重要なビタミンの1つである。しかしながら、その定量法すら十分に確立しておらず、摂取量およびその必要量も推測の域をでていないのが現状である。そこで、本研究においては、葉酸の全誘導体の特異的な定量法を確立を試みた。さらに、確立した定量法を用いて、生体内の葉酸、特に代謝の中心である肝臓中の濃度および分布を明らかにすることを目的とした。
1,葉酸およびその誘導体の定量法の確立
葉酸は、生体内では還元型となり、メチル化,ホルミル化等の反応により、8種類の誘導体として存在しているが、微量かつ化学的に非常に不安定であるため、その定量法すら十分に確立していなかった。本研究では、HPLC(カラム:μBondasphere Phenyl,移動相:50mM KH_2PO_4と0.1mM EDTAを含む12%メタノール(pH3.5)と電気化学検出器(+750mV,Ag/AgCl)を用いる方法により、8種類の葉酸誘導体(テトラヒドロ葉酸(THF),5-メチル-THF,5-ホルミル-THF,10-ホルミル-THF,5,10-メテニル-THF,5,10-メチレン-THF,プテロイルグルタミン酸,ジヒドロ葉酸)を特異的かつ高感度に定量することができた。
2,肝臓中の葉酸誘導体の定量
この確立した定量法を用いて、生体内の葉酸代謝の中心である肝臓中の葉酸誘導体の濃度およびその分布を明らかにすることを試みた。その結果、肝臓中には、6種類の葉酸誘導体が存在しており、10-ホルミル-THFが全体の53%をしめ、THF,5-メチル-THF,プテロイルグルタミン酸が全体の約15%であった。, kaken
対象リソースIRI

米胚乳タンパク質の機能性評価

2023, Principal investigator, 米胚乳タンパク質の機能性評価

2022, Principal investigator, 米胚乳タンパク質の機能性評価

2021 - 2025, Coinvestigator, ペプチドテクノロジーを基盤とした米タンパク質由来の新規高機能中分子ペプチド素材開発, 科学技術振興機構, 産学共創プラットフォーム共同研究推進プログラム（OPERA）

2021, Principal investigator, 米胚乳タンパク質の機能性評価

基盤研究（C）, 2019 - Mar. 2022, Principal investigator, 健康寿命に寄与する葉酸の機能解析と食生活への応用‐生涯を通した葉酸摂取の重要性‐

基盤研究（B）, 2019 - Mar. 2022, Coinvestigator, 摂食リズムの非同調による時計遺伝子の記憶が誘発する女性生殖機能異常の総合的解析

基盤研究（C）, 2019 - Mar. 2023, Coinvestigator, インゲン属雑豆の処理条件と含有レクチンの免疫賦活活性との関連と食生活への応用

■Ⅲ．社会連携活動実績

１．公的団体の委員等（審議会、国家試験委員、他大学評価委員，科研費審査委員等）

日本栄養改善学会, 評議員, 2006, 日本栄養改善学会, Society

日本ビタミン学会, 評議員, 2006, 日本ビタミン学会, Society

日本家政学会, 関西地区幹事（2006-2012, 2018-2021)、評議員、編集委員(2020-), 日本家政学会, Society

日本ポリフェノール学会, 評議員, Society

日本栄養・食糧学会, 近畿支部参与、JNSV編集委員, Society

NAKATA Rieko

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■Ⅱ．研究活動実績

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■Ⅲ．社会連携活動実績

１．公的団体の委員等（審議会、国家試験委員、他大学評価委員，科研費審査委員等）