Nutrients, MDPI AG, Screening Performance for Insufficient Vegetable Intake in Young Adult Japanese Women: Spot Urinary Potassium Excretion as a Simple, Non-Invasive Marker, Asaki Mizutani; Marina Yamagishi; Mami Sakuda; Ayuka Mita; Emi Morita; Naoko Suga; Atsuko Kitano; Rika Ohara; Akira Takamata; Junko Ishihara; Ribeka Takachi, Background/Objectives: Existing methods for assessing the intake of vegetables and fruits have limitations. This study aims to investigate the number of measurements required to screen individuals with insufficient usual vegetable intake or total fruit and vegetable intake using urinary potassium excretion estimated from spot urine samples, a non-invasive and simple biomarker. Methods: A total of 97 women aged 18–24 years provided a 12-day dietary survey, four 24 h urine collections, and three timed spot urine samples per day, collected in a duration of 4 days. Using the dietary surveys as a reference, we evaluated the number of spot urine samples required and their screening performance for identifying individuals whose vegetable intake or total fruit and vegetable intake was below the study population mean. Screening performance was assessed by receiver operating characteristic analysis and the area under the curve (AUC). Results: For vegetable intake, the AUCs were >0.70 when either a single spot urine sample was collected at the second urine void after waking or ≥2 spot urine samples were collected at arbitrary time points. For total fruit and vegetable intake, AUCs were >0.70 when using a single spot urine sample collected at the second void after waking up. Conclusions: These findings suggest that a time-specific single spot urine sample (i.e., the second urine void after waking) or ≥2 spot urine samples collected at arbitrary time points may help in screening individuals with habitually insufficient vegetable intake., 25 Feb. 2026, 18, 5, 735, 735, Scientific journal, 10.3390/nu18050735

Fitoterapia, Identification of components in coriander (Coriandrum sativum L.) inhibiting degranulation of RBL-2H3 cells., Rika Ohara; Takuya Sugahara; Yuna Sugie; Hiroyuki Onda; Nanami Yoshino; Kosuke Nishi; Momoko Ishida; Hiroe Kikuzaki, We found that a water-soluble extract of coriander (Coriandrum sativum L.) (leaves, petioles and stems) inhibits antigen-induced degranulation of RBL-2H3 cells, a rat basophil leukemia cell line. The aim of this study was to elucidate the anti-degranulation active components in the extract. The methanol-eluate fraction obtained by fractionation of the water-soluble extract using MCI gel column chromatography had strong activity, and eight components were isolated and identified. Two of them were identified as new compounds, (3S)-3-methyl-6-hydroxyisocoumarin 8-O-β-D-glucopyranoside (compound 1) and (7S,8R)-7,8-dihydro-8-β-D-glucopyranosyloxy-4-methoxy-7-methyl-5H-fro[2,3-g][2]benzopyran-5-one (compound 2). As a result of evaluation of anti-degranulation activity of eight components, seven of them, such as tryptophan, phenylalanine, dihydroxycoumarin glucoside, quercetin glycoside, rutin, compound 1, and compound 2, had the activity. These results indicated that the water-soluble extract of coriander contains several anti-degranulation substances., Nov. 2022, 163, 105298, 105298, Scientific journal, True, 10.1016/j.fitote.2022.105298

Molecules (Basel, Switzerland), Umbelliferose Isolated from Cuminum cyminum L. Seeds Inhibits Antigen-Induced Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells., Momoko Ishida; Rika Ohara; Fuka Miyagawa; Hiroe Kikuzaki; Kosuke Nishi; Hiroyuki Onda; Nanami Yoshino; Takuya Sugahara, Cuminum cyminum L. (cumin) is an annual plant of the Umbelliferae family native to Egypt. We previously showed that the aqueous extract of cumin seeds suppresses degranulation by downregulating the activation of antigen-induced intracellular signaling molecules in rat basophilic leukemia RBL-2H3 cells. However, the active substances in the extract have not yet been identified. Accordingly, herein, we aimed to ascertain the water-soluble substances present in cumin seeds that inhibit degranulation, which led to the identification of umbelliferose, a characteristic trisaccharide present in plants of the Umbelliferae family. Our study is the first to reveal the degranulation-suppressing activity of umbelliferose, and quantification studies suggest that cumin seed powder contains 1.6% umbelliferose. Raffinose, an isomer of umbelliferose, was also found to significantly suppress antigen-induced degranulation, but less so than umbelliferose. Both umbelliferose and raffinose contain sucrose subunits in their structures, with galactose moieties bound at different sites. These differences in structure suggest that the binding of galactose to the sucrose subunit at the α1-2 bond contributes to its strong degranulation-inhibiting properties., 25 Jun. 2022, 27, 13, Scientific journal, True, 10.3390/molecules27134101

Research Journal of Living Science, Elucidation of Food Factors based on Analytical Chemistry, Rika Ohara, Mar. 2024, 70, 2, 91, 98

Hiroe Kikuzaki; Rin Kondo; Rika Ohara; Ryoji Takagaki, Effects of cooking process on the average degree of polymerization of procyanidin in red sorghum grains, Poster presentation, 30 Aug. 2025, 30 Aug. 2025 - 31 Aug. 2025

〇Rika OHARA; Nozomi Tokuoka; Naho Tsumura; Hiroyuki ONDA; Tsuyoshi IKEDA; Hiroe KIKUZAKI, Changes in antioxidant activity of coriander with cooking manipulation, Others, 30 Aug. 2024, 29 Aug. 2024 - 31 Aug. 2024

◯Rika Ohara; Yuna Sugie; Momoko Ishida; Takuya Sugahara; Hiroyuki Onda; Nanami Yoshino; Hiroe Kikuzaki, Pacifichem 2021, Elucidation of components in coriander leaves and their functions, 18 Dec. 2021

◯Rika Ohara; Yurika Kitamura; Takuya Sugahara; Hiroyuki Onda; Nanami Yoshino; Hiroe Kikuzaki, ICoFF 2019, Constituents of coriander leaves involved in antioxidant and anti-allergic activities, 04 Dec. 2019

Oral presentation, 01 Sep. 2017

Mar. 2023

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Patent right, アレルギー治療用医薬組成物及び食品組成物, 菅原 卓也; 西 甲介; 石田 萌子; 菊﨑 泰枝; 小原 理加; 池田 剛; 恩田 浩幸; 吉野 七海, 特願2022-22489, 特開2023-119522, 特許第7801145号, 07 Jan. 2026, 16 Jan. 2026