Fitoterapia, Identification of components in coriander (Coriandrum sativum L.) inhibiting degranulation of RBL-2H3 cells., Rika Ohara; Takuya Sugahara; Yuna Sugie; Hiroyuki Onda; Nanami Yoshino; Kosuke Nishi; Momoko Ishida; Hiroe Kikuzaki, We found that a water-soluble extract of coriander (Coriandrum sativum L.) (leaves, petioles and stems) inhibits antigen-induced degranulation of RBL-2H3 cells, a rat basophil leukemia cell line. The aim of this study was to elucidate the anti-degranulation active components in the extract. The methanol-eluate fraction obtained by fractionation of the water-soluble extract using MCI gel column chromatography had strong activity, and eight components were isolated and identified. Two of them were identified as new compounds, (3S)-3-methyl-6-hydroxyisocoumarin 8-O-β-D-glucopyranoside (compound 1) and (7S,8R)-7,8-dihydro-8-β-D-glucopyranosyloxy-4-methoxy-7-methyl-5H-fro[2,3-g][2]benzopyran-5-one (compound 2). As a result of evaluation of anti-degranulation activity of eight components, seven of them, such as tryptophan, phenylalanine, dihydroxycoumarin glucoside, quercetin glycoside, rutin, compound 1, and compound 2, had the activity. These results indicated that the water-soluble extract of coriander contains several anti-degranulation substances., Nov. 2022, 163, 105298, 105298, Scientific journal, True, 10.1016/j.fitote.2022.105298

Molecules (Basel, Switzerland), Umbelliferose Isolated from Cuminum cyminum L. Seeds Inhibits Antigen-Induced Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells., Momoko Ishida; Rika Ohara; Fuka Miyagawa; Hiroe Kikuzaki; Kosuke Nishi; Hiroyuki Onda; Nanami Yoshino; Takuya Sugahara, Cuminum cyminum L. (cumin) is an annual plant of the Umbelliferae family native to Egypt. We previously showed that the aqueous extract of cumin seeds suppresses degranulation by downregulating the activation of antigen-induced intracellular signaling molecules in rat basophilic leukemia RBL-2H3 cells. However, the active substances in the extract have not yet been identified. Accordingly, herein, we aimed to ascertain the water-soluble substances present in cumin seeds that inhibit degranulation, which led to the identification of umbelliferose, a characteristic trisaccharide present in plants of the Umbelliferae family. Our study is the first to reveal the degranulation-suppressing activity of umbelliferose, and quantification studies suggest that cumin seed powder contains 1.6% umbelliferose. Raffinose, an isomer of umbelliferose, was also found to significantly suppress antigen-induced degranulation, but less so than umbelliferose. Both umbelliferose and raffinose contain sucrose subunits in their structures, with galactose moieties bound at different sites. These differences in structure suggest that the binding of galactose to the sucrose subunit at the α1-2 bond contributes to its strong degranulation-inhibiting properties., 25 Jun. 2022, 27, 13, Scientific journal, True, 10.3390/molecules27134101

Research Journal of Living Science, Elucidation of Food Factors based on Analytical Chemistry, Rika Ohara, Mar. 2024, 70, 2, 91, 98

〇Rika OHARA; Nozomi Tokuoka; Naho Tsumura; Hiroyuki ONDA; Tsuyoshi IKEDA; Hiroe KIKUZAKI, Changes in antioxidant activity of coriander with cooking manipulation, 30 Aug. 2024, 29 Aug. 2024 - 31 Aug. 2024

◯Rika Ohara; Yuna Sugie; Momoko Ishida; Takuya Sugahara; Hiroyuki Onda; Nanami Yoshino; Hiroe Kikuzaki, Pacifichem 2021, Elucidation of components in coriander leaves and their functions, 18 Dec. 2021

◯Rika Ohara; Yurika Kitamura; Takuya Sugahara; Hiroyuki Onda; Nanami Yoshino; Hiroe Kikuzaki, ICoFF 2019, Constituents of coriander leaves involved in antioxidant and anti-allergic activities, 04 Dec. 2019

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