Refereed, Annals of Botany, Oxford University Press (OUP), Callose deficiency modulates plasmodesmata frequency and extracellular distance in rice pollen mother and tapetal cells, Harsha Somashekar; Keiko Takanami; Yoselin Benitez-Alfonso; Akane Oishi; Rie Hiratsuka; Ken-Ichi Nonomura, Abstract

Background and Aims

Fertilization relies on pollen mother cells able to transition from mitosis to meiosis to supply gametes. This process involves remarkable changes at the molecular, cellular and physiological levels, including (but not limited to) remodelling of the cell wall. During the onset of meiosis, the cellulose content in the pollen mother cell walls gradually declines, with the concurrent deposition of the polysaccharide callose in anther locules. We aim to understand the biological significance of cellulose-to-callose turnover in pollen mother cells walls.

Methods

We carried out electron microscopic, aniline blue and renaissance staining analyses of rice flowers.

Key Results

Our observations indicate that in wild-type rice anthers, the mitosis-to-meiosis transition coincides with a gradual reduction in the number of cytoplasmic connections called plasmodesmata. A mutant in the Oryza sativa callose synthase GSL5 (Osgsl5-3), impaired in callose accumulation in premeiotic and meiotic anthers, displayed a greater reduction in plasmodesmata frequency among pollen mother cells and tapetal cells, suggesting a role for callose in maintenance of plasmodesmata. In addition, a significant increase in extracellular distance between pollen mother cells and impaired premeiotic cell shaping was observed in the Osgsl5-3 mutant.

Conclusions

The results suggest that callose-to-cellulose turnover during the transition from mitosis to meiosis is necessary to maintain cell-to-cell connections and optimal extracellular distance among the central anther locular cells. The findings of this study contribute to our understanding of the regulatory influence of callose metabolism during initiation of meiosis in flowering plants., Aug. 2024, Scientific journal, 10.1093/aob/mcae137

Refereed, Frontiers in Molecular Neuroscience, Frontiers Media SA, Function of gastrin-releasing peptide receptors in ocular itch transmission in the mouse trigeminal sensory system, Keiko Takanami; Masaya Kuroiwa; Ren Ishikawa; Yuji Imai; Akane Oishi; Midori Hashino; Yasushi Shimoda; Hirotaka Sakamoto; Tsuyoshi Koide, The prevalence of allergic conjunctivitis in itchy eyes has increased constantly worldwide owing to environmental pollution. Currently, anti-allergic and antihistaminic eye drops are used; however, there are many unknown aspects about the neural circuits that transmit itchy eyes. We focused on the gastrin-releasing peptide (GRP) and GRP receptor (GRPR), which are reportedly involved in itch transmission in the spinal somatosensory system, to determine whether the GRP system is involved in itch neurotransmission of the eyes in the trigeminal sensory system. First, the instillation of itch mediators, such as histamine (His) and non-histaminergic itch mediator chloroquine (CQ), exhibited concentration-dependent high levels of eye scratching behavior, with a significant sex differences observed in the case of His. Histological analysis revealed that His and CQ significantly increased the neural activity of GRPR-expressing neurons in the caudal part of the spinal trigeminal nucleus of the medulla oblongata in GRPR transgenic mice. We administered a GRPR antagonist or bombesin-saporin to ablate GRPR-expressing neurons, followed by His or CQ instillation, and observed a decrease in CQ-induced eye-scratching behavior in the toxin experiments. Intracisternal administration of neuromedin C (NMC), a GRPR agonist, resulted in dose-dependent excessive facial scratching behavior, despite the absence of an itch stimulus on the face. To our knowledge, this is the first study to demonstrate that non-histaminergic itchy eyes were transmitted centrally via GRPR-expressing neurons in the trigeminal sensory system, and that NMC in the medulla oblongata evoked facial itching., Nov. 2023, 16, 1280024, Scientific journal, 10.3389/fnmol.2023.1280024

Refereed, RESEARCH JOURNAL OF LIVING SCIENCE, Itch sensation that fluctuates due to environmental and psychological factors during the course of life, Takanami Keiko, Oct. 2023, 70, 1, 15, 20

Refereed, General and Comparative Endocrinology, Elsevier BV, Chronic corticosterone exposure evokes itch hypersensitivity and sexual dysfunction in male rats: Relationship between the two distinct gastrin-releasing peptide systems in the spinal cord, Keiko Takanami; Makoto Morishita; Tatsuya Sakamoto; Hirotaka Sakamoto, Aug. 2023, 339, 114289, 114289, Scientific journal, 10.1016/j.ygcen.2023.114289

Refereed, Histaminergic and non-histaminergic itch: From channels to behavior. Nova science publishers, Gastrin-Releasing Peptide and Gastrin-Releasing Peptide Receptor Expressing Neurons (Chapter 5), Keiko Takanami, Jul. 2023, 93, 108

Refereed, Journal of Comparative Neurology, Wiley, Characterization of the expression of gastrin‐releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans, Keiko Takanami; Takumi Oti; Yasuhisa Kobayashi; Koki Hasegawa; Takashi Ito; Naoaki Tsutsui; Yasumasa Ueda; Earl Carstens; Tatsuya Sakamoto; Hirotaka Sakamoto, Nov. 2022, 530, 16, 2804, 2819, Scientific journal, 10.1002/cne.25376

Refereed, Proceedings of the Royal Society B: Biological Sciences, The Royal Society, Footedness for scratching itchy eyes in rodents, Yukitoshi Katayama; Ayane Miura; Tatsuya Sakamoto; Keiko Takanami; Hirotaka Sakamoto, The neural bases of itchy eye transmission remain unclear compared with those involved in body itch. Here, we show in rodents that the gastrin-releasing peptide receptor (GRPR) of the trigeminal sensory system is involved in the transmission of itchy eyes. Interestingly, we further demonstrate a difference in scratching behaviour between the left and right hindfeet in rodents; histamine instillation into the conjunctival sac of both eyes revealed right-foot biased laterality in the scratching movements. Unilateral histamine instillation specifically induced neural activation in the ipsilateral sensory pathway, with no significant difference between the activations following left- and right-eye instillations. Thus, the behavioural laterality is presumably due to right-foot preference in rodents. Genetically modified rats with specific depletion of Grpr- expressing neurons in the trigeminal sensory nucleus caudalis of the medulla oblongata exhibited fewer and shorter histamine-induced scratching movements than controls and eliminated the footedness. These results taken together indicate that the Grpr -expressing neurons are required for the transmission of itch sensation from the eyes, but that foot preference is generated centrally. These findings could open up a new field of research on the mechanisms of the laterality in vertebrates and also offer new potential therapeutic approaches to refractory pruritic eye disorders., 26 Oct. 2022, 289, 1985, 20221126, 20221126, Scientific journal, True, 10.1098/rspb.2022.1126

Refereed, Communications Biology, Springer Science and Business Media LLC, Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals, Ayumu Inutsuka; Sho Maejima; Hiroyuki Mizoguchi; Ryosuke Kaneko; Rei Nomura; Keiko Takanami; Hirotaka Sakamoto; Tatsushi Onaka, Abstract

Transgenic animals expressing fluorescent proteins are widely used to label specific cells and proteins. By using a split Cre recombinase fused with mCherry-binding nanobodies or designed ankyrin repeat proteins, we created Cre recombinase dependent on red fluorescent protein (RFP) (Cre-DOR). Functional binding units for monomeric RFPs are different from those for polymeric RFPs. We confirmed selective target RFP-dependent gene expression in the mouse cerebral cortex using stereotaxic injection of adeno-associated virus vectors. In estrogen receptor-beta (Esr2)-mRFP1 mice and gastrin-releasing peptide receptor (Grpr)-mRFP1 rats, we confirmed that Cre-DOR can be used for selective tracing of the neural projection from RFP-expressing specific neurons. Cellular localization of RFPs affects recombination efficiency of Cre-DOR, and light and chemical-induced nuclear translocation of an RFP-fused protein can modulate Cre-DOR efficiency. Our results provide a method for manipulating gene expression in specific cells expressing RFPs and expand the repertory of nanobody-based genetic tools., 16 Sep. 2022, 5, 1, 979, 979, Scientific journal, True, 10.1038/s42003-022-03944-2

Refereed, eLife, eLife Sciences Publications, Ltd, Inhibition of itch by neurokinin 1 receptor (Tacr1) -expressing ON cells in the rostral ventromedial medulla in mice, Taylor Follansbee; Dan Domocos; Eileen Nguyen; Amanda Nguyen; Aristea Bountouvas; Lauren Velasquez; Mirela Iodi Carstens; Keiko Takanami; Sarah E Ross; Earl Carstens, The rostral ventromedial medulla (RVM) is important in descending modulation of spinal nociceptive transmission, but it is unclear if the RVM also modulates spinal pruriceptive transmission. RVM ON cells are activated by noxious algesic and pruritic stimuli and are pronociceptive. Many RVM-spinal projection neurons express the neurokinin-1 receptor (Tacr1), and ON-cells are excited by local administration of substance P (SP). We hypothesized that Tacr1-expressing RVM ON cells exert an inhibitory effect on itch opposite to their pronociceptive action. Intramedullary microinjection of SP significantly potentiated RVM ON cells and reduced pruritogen-evoked scratching while producing mild mechanical sensitization. Chemogenetic activation of RVM Tacr1-expressing RVM neurons also reduced acute pruritogen-evoked scratching. Optotagging experiments confirmed RVM Tacr1-expressing neurons to be ON cells. We conclude that Tacr1-expressing ON cells in RVM play a significant role in the modulation of pruriceptive transmission., 16 Aug. 2022, 11, e69626, Scientific journal, 10.7554/elife.69626

Refereed, Science (New York, N.Y.), A spinal microglia population involved in remitting and relapsing neuropathic pain., Keita Kohno; Ryoji Shirasaka; Kohei Yoshihara; Satsuki Mikuriya; Kaori Tanaka; Keiko Takanami; Kazuhide Inoue; Hirotaka Sakamoto; Yasuyuki Ohkawa; Takahiro Masuda; Makoto Tsuda, Neuropathic pain is often caused by injury and diseases that affect the somatosensory system. Although pain development has been well studied, pain recovery mechanisms remain largely unknown. Here, we found that CD11c-expressing spinal microglia appear after the development of behavioral pain hypersensitivity following nerve injury. Nerve-injured mice with spinal CD11c+ microglial depletion failed to recover spontaneously from this hypersensitivity. CD11c+ microglia expressed insulin-like growth factor-1 (IGF1), and interference with IGF1 signaling recapitulated the impairment in pain recovery. In pain-recovered mice, the depletion of CD11c+ microglia or the interruption of IGF1 signaling resulted in a relapse in pain hypersensitivity. Our findings reveal a mechanism for the remission and recurrence of neuropathic pain, providing potential targets for therapeutic strategies., Apr. 2022, 376, 6588, 86, 90, Scientific journal, True, 10.1126/science.abf6805

Refereed, Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Estrogens influence female itch sensitivity via the spinal gastrin-releasing peptide receptor neurons, Keiko Takanami; Daisuke Uta; Ken Ichi Matsuda; Mitsuhiro Kawata; Earl Carstens; Tatsuya Sakamoto; Hirotaka Sakamoto, There are sex differences in somatosensory sensitivity. Circulating estrogens appear to have a pronociceptive effect that explains why females are reported to be more sensitive to pain than males. Although itch symptoms develop during pregnancy in many women, the underlying mechanism of female-specific pruritus is unknown. Here, we demonstrate that estradiol, but not progesterone, enhances histamine-evoked scratching behavior indicative of itch in female rats. Estradiol increased the expression of the spinal itch mediator, gastrin-releasing peptide (GRP), and increased the histamine-evoked activity of itch-processing neurons that express the GRP receptor (GRPR) in the spinal dorsal horn. The enhancement of itch behavior by estradiol was suppressed by intrathecal administration of a GRPR blocker. In vivo electrophysiological analysis showed that estradiol increased the histamine-evoked firing frequency and prolonged the response of spinal GRP-sensitive neurons in female rats. On the other hand, estradiol did not affect the threshold of noxious thermal pain and decreased touch sensitivity, indicating that estradiol separately affects itch, pain, and touch modalities. Thus, estrogens selectively enhance histamine-evoked itch in females via the spinal GRP/GRPR system. This may explain why itch sensation varies with estrogen levels and provides a basis for treating itch in females by targeting GRPR., Aug. 2021, 118, 31, e2103536118, e2103536118, Scientific journal, True, 10.1073/pnas.2103536118

Refereed, Scientific Reports, Springer Science and Business Media LLC, The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus, Asuka Hirooka; Mayuko Hamada; Daiki Fujiyama; Keiko Takanami; Yasuhisa Kobayashi; Takumi Oti; Yukitoshi Katayama; Tatsuya Sakamoto; Hirotaka Sakamoto, Bombesin is a putative antibacterial peptide isolated from the skin of the frog, Bombina bombina. Two related (bombesin-like) peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) have been found in mammals. The history of GRP/bombesin discovery has caused little attention to be paid to the evolutionary relationship of GRP/bombesin and their receptors in vertebrates. We have classified the peptides and their receptors from the phylogenetic viewpoint using a newly established genetic database and bioinformatics. Here we show, by using a clawed frog (Xenopus tropicalis), that GRP is not a mammalian counterpart of bombesin and also that, whereas the GRP system is widely conserved among vertebrates, the NMB/bombesin system has diversified in certain lineages, in particular in frog species. To understand the derivation of GRP system in the ancestor of mammals, we have focused on the GRP system in Xenopus. Gene expression analyses combined with immunohistochemistry and Western blotting experiments demonstrated that GRP peptides and their receptors are distributed in the brain and stomach of Xenopus. We conclude that GRP peptides and their receptors have evolved from ancestral (GRP-like peptide) homologues to play multiple roles in both the gut and the brain as one of the ‘gut-brain peptide’ systems., Jun. 2021, 11, 1, 13315, 13315, Scientific journal, True, 10.1038/s41598-021-92528-x

Refereed, Journal of Comparative Neurology, Wiley, Variation of pro‐vasopressin processing in parvocellular and magnocellular neurons in the paraventricular nucleus of the hypothalamus: Evidence from the vasopressin‐related glycopeptide copeptin, Natsuko Kawakami; Akito Otubo; Sho Maejima; Ashraf H. Talukder; Keita Satoh; Takumi Oti; Keiko Takanami; Yasumasa Ueda; Keiichi Itoi; John F. Morris; Tatsuya Sakamoto; Hirotaka Sakamoto, May 2021, Scientific journal, 10.1002/cne.25026

Refereed, Current Biology, Elsevier BV, Oxytocin Influences Male Sexual Activity via Non-synaptic Axonal Release in the Spinal Cord, Takumi Oti; Keita Satoh; Daisuke Uta; Junta Nagafuchi; Sayaka Tateishi; Ryota Ueda; Keiko Takanami; Larry J. Young; Antony Galione; John F. Morris; Tatsuya Sakamoto; Hirotaka Sakamoto, Jan. 2021, 31, 1, 103, 114.e5, Scientific journal, 10.1016/j.cub.2020.09.089

Refereed, Neuroscience, Elsevier BV, Detection and Characterization of Estrogen Receptor Beta Expression in the Brain with Newly Developed Transgenic Mice, Shoko Sagoshi; Sho Maejima; Masahiro Morishita; Satoshi Takenawa; Akito Otubo; Keiko Takanami; Tatsuya Sakamoto; Hirotaka Sakamoto; Shinji Tsukahara; Sonoko Ogawa, Two types of nuclear estrogen receptors, ERα and ERβ, have been shown to be differentially involved in the regulation of various types of behaviors. Due to a lack of tools for identifying ERβ expression, detailed anatomical distribution and neurochemical characteristics of ERβ expressing cells and cellular co-expression with ERα remain unclear. We have generated transgenic mice ERβ-RFPtg, in which RFP was inserted downstream of ERβ BAC promotor. We verified RFP signals as ERβ by confirming: (1) high ERβ mRNA levels in RFP-expressing cells collected by fluorescence-activated cell sorting; and (2) co-localization of ERβ mRNA and RFP proteins in the paraventricular nucleus (PVN). Strong ERβ-RFP signals were found in the PVN, medial preoptic area (MPOA), bed nucleus of the stria terminalis, medial amygdala (MeA), and dorsal raphe nucleus (DRN). In the MPOA and MeA, three types of cell populations were identified; those expressing both ERα and ERβ, and those expressing exclusively either ERα or ERβ. The majority of PVN and DRN cells expressed only ERβ-RFP. Further, ERβ-RFP positive cells co-expressed oxytocin in the PVN, and tryptophan hydroxylase 2 and progesterone receptors in the DRN. In the MeA, some ERβ-RFP positive cells co-expressed oxytocin receptors. These findings collectively suggest that ERβ-RFPtg mice can be a powerful tool for future studies on ERβ function in the estrogenic regulation of social behaviors., Jul. 2020, 438, 182, 197, Scientific journal, True, 10.1016/j.neuroscience.2020.04.047

Refereed, Frontiers in Physiology,, The Amphibious Mudskipper: A Unique Model Bridging the Gap of Central Actions of Osmoregulatory Hormones Between Terrestrial and Aquatic Vertebrates., Katayama K; Sakamoto T; Takanami K; Takei Y, Aug. 2018, 9, 1112

Refereed, Endocrinology, Effects of sex steroids on the spinal gastrin-releasing peptide system controlling male sexual function in rats., Oti T; Takanami K; Ito S; Ueda T; Matsuda KI; Kawata M; Soh J; Ukimura O; Sakamoto T; Sakamoto H, The gastrin-releasing peptide (GRP) system in the lumbosacral spinal cord controls male sexual function in rats. In contrast, in female rats, GRP neurons could scarcely be detected around puberty when circulating ovarian steroid hormones such as estradiol and progesterone levels are increasing. However, little information is available on feminizing or demasculinizing effects of ovarian steroids on the central nervous system in female puberty and adulthood. In this study, to visualize the spinal GRP neurons in vivo, we generated a GRP-promoter-Venus transgenic (Tg) rat line and studied the effects of the sex steroid hormones on GRP expression in the rat lumbar cord by examining the Venus fluorescence. In these Tg rats, the sexually dimorphic spinal GRP neurons controlling male sexual function were clearly labeled with Venus fluorescence. As expected, Venus fluorescence in the male lumbar cord was markedly decreased after castration and restored by chronic androgen replacement. Furthermore, androgen-induced Venus expression in the spinal cord of adult Tg males was significantly attenuated by chronic treatment with progesterone but not with estradiol. A luciferase assay using a human GRP-promoter construct showed that androgens enhance the spinal GRP system, and more strikingly, that progesterone acts to inhibit the GRP system via an androgen receptor-mediated mechanism. These results demonstrate that circulating androgens may play an important role in the spinal GRP system controlling male sexual function not only in rats but also in humans and that progesterone could be an important feminizing factor in the spinal GRP system in females during pubertal development., Apr. 2018, 159, 4, 1886, 1896, Scientific journal, True, 10.1210/en.2018-00043

Refereed, Spinal Cord, Nature Publishing Group, A sexually dimorphic peptidergic system in the lower spinal cord controlling penile function in non-human primates, T. Ito; T. Oti; K. Takanami; K. Satoh; Y. Ueda; T. Sakamoto; H. Sakamoto, 2018, 56, 1, 57, 62, Scientific journal, 10.1038/sc.2017.105

Refereed, JOURNAL OF COMPARATIVE NEUROLOGY, Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus), Kei Tamura; Yasuhisa Kobayashi; Asuka Hirooka; Keiko Takanami; Takumi Oti; Takamichi Jogahara; Sen-ichi Oda; Tatsuya Sakamoto; Hirotaka Sakamoto, May 2017, 525, 7, 1586, 1598, Scientific journal, 10.1002/cne.24138

Refereed, NEUROSCIENCE LETTERS, Decrease in neuronal spine density in the postpartum period in the amygdala and bed nucleus of the stria terminalis in rat, Seiki Matsuo; Ken Ichi Matsuda; Keiko Takanami; Taisuke Mori; Masaki Tanaka; Mitsuhiro Kawata; Jo Kitawaki, Feb. 2017, 641, 21, 25, Scientific journal, 10.1016/j.neulet.2017.01.040

Refereed, Biological psychiatry, Activation of Supraoptic Oxytocin Neurons by Secretin Facilitates Social Recognition., Yuki Takayanagi; Masahide Yoshida; Akihide Takashima; Keiko Takanami; Shoma Yoshida; Katsuhiko Nishimori; Ichiko Nishijima; Hirotaka Sakamoto; Takanori Yamagata; Tatsushi Onaka, BACKGROUND: Social recognition underlies social behavior in animals, and patients with psychiatric disorders associated with social deficits show abnormalities in social recognition. Oxytocin is implicated in social behavior and has received attention as an effective treatment for sociobehavioral deficits. Secretin receptor-deficient mice show deficits in social behavior. The relationship between oxytocin and secretin concerning social behavior remains to be determined. METHODS: Expression of c-Fos in oxytocin neurons and release of oxytocin from their dendrites after secretin application were investigated. Social recognition was examined after intracerebroventricular or local injection of secretin, oxytocin, or an oxytocin receptor antagonist in rats, oxytocin receptor-deficient mice, and secretin receptor-deficient mice. Electron and light microscopic immunohistochemical analysis was also performed to determine whether oxytocin neurons extend their dendrites into the medial amygdala. RESULTS: Supraoptic oxytocin neurons expressed the secretin receptor. Secretin activated supraoptic oxytocin neurons and facilitated oxytocin release from dendrites. Secretin increased acquisition of social recognition in an oxytocin receptor-dependent manner. Local application of secretin into the supraoptic nucleus facilitated social recognition, and this facilitation was blocked by an oxytocin receptor antagonist injected into, but not outside of, the medial amygdala. In the medial amygdala, dendrite-like thick oxytocin processes were found to extend from the supraoptic nucleus. Furthermore, oxytocin treatment restored deficits of social recognition in secretin receptor-deficient mice. CONCLUSIONS: The results of our study demonstrate that secretin-induced dendritic oxytocin release from supraoptic neurons enhances social recognition. The newly defined secretin-oxytocin system may lead to a possible treatment for social deficits., 01 Feb. 2017, 81, 3, 243, 251, True, 10.1016/j.biopsych.2015.11.021

Refereed, PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, Postnatal development of the gastrin-releasing peptide system in the lumbosacral spinal cord controlling male reproductive function in rats, Nao Katayama; Takumi Oti; Keiko Takanami; Tatsuya Sakamoto; Hirotaka Sakamoto, Feb. 2016, 92, 2, 69, 75, Scientific journal, 10.2183/pjab.92.69

Refereed, BIOLOGY OF SEX DIFFERENCES, Perinatal testosterone exposure is critical for the development of the male-specific sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that mediates erection and ejaculation, Takumi Oti; Keiko Takanami; Nao Katayama; Tomoca Edey; Keita Satoh; Tatsuya Sakamoto; Hirotaka Sakamoto, Jan. 2016, 7, 4, Scientific journal, 10.1186/s13293-016-0058-x

Refereed, ACTA HISTOCHEMICA ET CYTOCHEMICA, Expression of Sex Steroid Hormone Receptors in Vagal Motor Neurons Innervating the Trachea and Esophagus in Mouse, Shigeyuki Mukudai; Ken Ichi Matsuda; Hideki Bando; Keiko Takanami; Takeshi Nishio; Yoichiro Sugiyama; Yasuo Hisa; Mitsuhiro Kawata, 2016, 49, 1, 37, 46, Scientific journal, 10.1267/ahc.15037

Refereed, ACTA HISTOCHEMICA ET CYTOCHEMICA, Comparative Anatomy of Gastrin-releasing Peptide Pathways in the Trigeminal Sensory System of Mouse and the Asian House Musk Shrew Suncus murinus, Keiko Takanami; Kaihei Inoue; Hiroki Mukai; Kei Tamura; Takamichi Jogahara; Sen-ichi Oda; Mitsuhiro Kawata; Tatsuya Sakamoto; Hirotaka Sakamoto, 2016, 49, 6, 181, 190, Scientific journal, 10.1267/ahc.16030

Refereed, NEUROSCIENCE LETTERS, Critical role of androgen receptor in the postnatal period in male sexual behavior in rats, Shunji Yamada; Miku Ohoya; Keiko Takanami; Ken Ichi Matsuda; Mitsuhiro Kawata, Nov. 2015, 609, 189, 193, Scientific journal, 10.1016/j.neulet.2015.10.040

Refereed, Data in Brief, Elsevier Inc., Three-dimensional visualization of multiple synapses in thick sections using high-voltage electron microscopy in the rat spinal cord, Keita Satoh†; Keiko Takanami†; Kazuyoshi Murata; Mitsuhiro Kawata; Tatsuya Sakamoto; Hirotaka Sakamoto (†:co-first author), 01 Sep. 2015, 4, 566, 570, Scientific journal, 10.1016/j.dib.2015.07.005

Refereed, NEUROSCIENCE LETTERS, Effective synaptome analysis of itch-mediating neurons in the spinal cord: A novel immunohistochemical methodology using high-voltage electron microscopy, Keita Satoh†; Keiko Takanami†; Kazuyoshi Murata; Mitsuhiro Kawata; Tatsuya Sakamoto; Hirotaka Sakamoto (†:co-first author), Jul. 2015, 599, 86, 91, Scientific journal, 10.1016/j.neulet.2015.05.031

Refereed, JOURNAL OF COMPARATIVE NEUROLOGY, Distribution of Gastrin-Releasing Peptide in the Rat Trigeminal and Spinal Somatosensory Systems, Keiko Takanami; Hirotaka Sakamoto; Ken Ichi Matsuda; Keita Satoh; Takashi Tanida; Shunji Yamada; Kaihei Inoue; Takumi Oti; Tatsuya Sakamoto; Mitsuhiro Kawata, Jun. 2014, 522, 8, 1858, 1873, Scientific journal, 10.1002/cne.23506

Refereed, CURRENT NEUROPHARMACOLOGY, The Gastrin-Releasing Peptide Receptor (GRPR) in the Spinal Cord as a Novel Pharmacological Target, Keiko Takanami; Hirotaka Sakamoto, 2014, 12, 5, 434, 443, Scientific journal

Refereed, GLIA, G protein-coupled receptor 30 contributes to improved remyelination after cuprizone-induced demyelination, Yukie Hirahara; Ken Ichi Matsuda; Hisao Yamada; Akira Saitou; Shinsuke Morisaki; Keiko Takanami; Joan M. Boggs; Mitsuhiro Kawata, Mar. 2013, 61, 3, 420, 431, Scientific journal, 10.1002/glia.22445

Refereed, BRAIN RESEARCH, Testosterone has sublayer-specific effects on dendritic spine maturation mediated by BDNF and PSD-95 in pyramidal neurons in the hippocampus CA1 area, Meihua Li; Miwako Masugi-Tokita; Keiko Takanami; Shunji Yamada; Mitsuhiro Kawata, Nov. 2012, 1484, 76, 84, Scientific journal, 10.1016/j.brainres.2012.09.028

Refereed, FRONTIERS IN BIOSCIENCE-LANDMARK, Rapid signaling of steroid hormones in the vertebrate nervous system, Hirotaka Sakamoto; Hideya Takahashi; Ken-Ichi Matsuda; Mayumi Nishi; Keiko Takanami; Maho Ogoshi; Tatsuya Sakamoto; Mitsuhiro Kawata, Jan. 2012, 17, 996, 1019, Scientific journal, 10.2741/3970

Refereed, BRAIN RESEARCH, Expression of G protein-coupled receptor 30 in the spinal somatosensory system, Keiko Takanami; Hirotaka Sakamoto; Ken-Ichi Matsuda; Koji Hosokawa; Mayumi Nishi; Eric R. Prossnitz; Mitsuhiro Kawata, Jan. 2010, 1310, 17, 28, Scientific journal, 10.1016/j.brainres.2009.11.004

Refereed, ENDOCRINOLOGY, Androgen Regulates the Sexually Dimorphic Gastrin-Releasing Peptide System in the Lumbar Spinal Cord that Mediates Male Sexual Function, Hirotaka Sakamoto; Keiko Takanami; Damian G. Zuloaga; Ken-ichi Matsuda; Cynthia L. Jordan; S. Marc Breedlove; Mitsuhiro Kawata, Aug. 2009, 150, 8, 3672, 3679, Scientific journal, 10.1210/en.2008-1791

Refereed, PLOS ONE, Stress Affects a Gastrin-Releasing Peptide System in the Spinal Cord That Mediates Sexual Function: Implications for Psychogenic Erectile Dysfunction, Hirotaka Sakamoto; Ken-Ichi Matsuda; Damian G. Zuloaga; Nobuko Nishiura; Keiko Takanami; Cynthia L. Jordan; S. Marc Breedlove; Mitsuhiro Kawata, Jan. 2009, 4, 1, e4276, Scientific journal, 10.1371/journal.pone.0004276

Refereed, JOURNAL OF NEUROENDOCRINOLOGY, Steroid receptor signalling in the brain - Lessons learned from molecular imaging, M. Kawata; M. Nishi; K. Matsuda; H. Sakamoto; N. Kaku; M. Masugi-Tokita; K. Fujikawa; Y. Hirahara-Wada; K. Takanami; H. Mori, Jun. 2008, 20, 6, 673, 676, 10.1111/j.1365-2826.2008.01727.x

Refereed, Jan. 2025, 61, 1, 50, 54

Refereed, Oct. 2023, 139, 70, 15, 20

Refereed, Jan. 2025, 61, 1, 50, 54

Refereed, Sep. 2021

Not Refereed, Oct. 2020, 69, 4, 142, 143

Refereed, ACTA DERMATO-VENEREOLOGICA, Morphological and molecular evolutional analyses of itch focused on the gastrin-releasing peptide system in mammals, Keiko Takanami; Keita Satoh; Kazuyoshi Murata; Tatsuya Sakamoto; Hirotaka Sakamoto, Sep. 2017, 97, 8, 1049, 1050

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Invited oral presentation, Mar. 2025

Invited oral presentation, Nov. 2024

Invited oral presentation, Oct. 2024

Poster presentation, Oct. 2024

Poster presentation, Oct. 2024

Keiko Takanami; Masaya Kuroiwa; Ren Ishikawa; Yuji Imai; Akane Oishi; Midori Hashino; Yasushi Shimoda; HirotakaSakamoto; Tsuyoshi Koide, The 47th Annual Meeting of the Japan Neuroscience Society (NEURO 2024), Function of gastrin-releasing peptide receptors in ocular itchtransmission in the trigeminal sensory system, Poster presentation, Jul. 2024

Invited oral presentation, Jun. 2024

Invited oral presentation, Dec. 2023

Invited oral presentation, Nov. 2023

Invited oral presentation, Oct. 2023

Invited oral presentation, Oct. 2023

Invited oral presentation, Aug. 2023

Invited oral presentation, Jun. 2023

Invited oral presentation, May 2023

Oral presentation, Mar. 2023

Invited oral presentation, Mar. 2023

Oral presentation, Mar. 2023

Invited oral presentation, Jan. 2023

Invited oral presentation, Oct. 2022

Keiko TAKANAMI; Yukitoshi KATAYAMA; Tatsuya SAKAMOTO; Hirotaka SAKAMOTO, Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology, Symposia “Stress Axis: Molecular and Cellular Regulation of the HPI/HPA Axis”, Itch and its molecular/functional evolution, Invited oral presentation, Sep. 2022

Invited oral presentation, Jul. 2022

Keynote oral presentation, Apr. 2022

Public symposium, Mar. 2022

Oral presentation, Feb. 2022

Public discourse, Jan. 2022

Poster presentation, Dec. 2021

Poster presentation, Nov. 2021

Oral presentation, Oct. 2021

Oral presentation, Oct. 2021

Oral presentation, Oct. 2021

Keiko Takanami, International Federation of Societies for Histochemistry and Cytochemistry, IFSHC Workshop 2021, Session Japan, Histochemical challenges to the itch neurotransmission and evolution, Nominated symposium, Sep. 2021

Poster presentation, Jul. 2021

Keiko Takanami; Tsuyoshi Koide, The 44th Annual Meeting of the Japan Neuroscience Society, Species and strain differences in the itch threshold in rodents, Poster presentation, Jul. 2021

Oral presentation, May 2021

Keiko Takanami; Tsuyoshi Koide, The 68th Annual Meeting of Japanese Association for Laboratory Animal Science, Mouse strain difference in the sensory threshold, Oral presentation, May 2021

Nominated symposium, Apr. 2021

Keiko Takanami, Approach from morphology of molecular and functional evolution of itch, Public symposium, Mar. 2021

Nominated symposium, 19 Dec. 2020

Sho Maejima; Rei Nomura; Keiko Takanami; Tatsuya Sakamoto; Hirotaka Sakamoto, The gastrin-releasing peptide system in the medial preoptic area controls male sexual activity in rats, Mar. 2020

TAKANAMI Keiko; MORISHITA Makoto; SAKAMOTO Tatsuya; SAKAMOTO Hirotaka, 10th World Congress of Itch, Chronic stress affects itch sensitivity and male sexual function in rats, Oral presentation, Nov. 2019

K Takanami; D Fujiyama; A Hirooka; H Sakamoto, THE 48th NAITO CONFERENCE on Integrated Sensory Sciences―Pain, Itch, Smell and Taste, Molecular and functional evolutionary analysis of itch focused on the gastrin-releasing peptide system in vertebrate, Poster presentation, Oct. 2019

Others, Oct. 2019

Oral presentation, Sep. 2019

Nominated symposium, Sep. 2019

Effects of sociality on; the; social contagious behavior, The 42th Annual Meeting of the Japan Neuroscience Society, K Takanami, O Yakimenko, H Nagayama, T Koide, Poster presentation, Jul. 2019

Poster presentation, May 2019

K Takanami; O Yakimenko; H Nagayama; T Koide, The 3rd Sino-Japan Symposium on the Frontier of Behavioral Neuroendocrinology, DOES SOCIALITY AFFECT THE SOCIAL CONTAGIOUS BEHAVIOR?, Poster presentation, Mar. 2019

Public discourse, Mar. 2019

TAKANAMI Keiko; SAKAMOTO Hirotaka; KOIDE Tsuyoshi, Comparative analysis of itch transmission using mouse and rat, Public symposium, Nov. 2018

Oral presentation, Oct. 2018

Oral presentation, Oct. 2018

Oral presentation, Sep. 2018

Oral presentation, Sep. 2018

Makoto Morishita; Keiko Takanami; Tatsuya Sakamoto; Hirotaka Sakamoto, 9th International Congress of Neuroendocrinology, Effects of chronic corticosterone administration on male sexual function and itch sensation in rats, Poster presentation, Jul. 2018

K Takanami; K Murata; T Sakamoto; H Sakamoto, 9th International Congress of Neuroendocrinology, Ultrastructural analysis of the peptidergic neurons mediating itch sensation, Poster presentation, Jul. 2018

Oral presentation, May 2018

高浪景子; 伊藤隆志; 越智拓海; 小林靖尚; 佐藤慧太; 上田康雅; 坂本竜哉; 坂本浩隆, 第123回日本解剖学会総会・全国学術集会, 霊長類ニホンザルにおけるGastrin-releasing peptide神経系の機能局在, Poster presentation, Mar. 2018

Poster presentation, Dec. 2017

Keiko Takanami, National Institute of Genetics, Biological Symposium, Gastrin-releasing peptide system as itch neural circuits in rodents, Nov. 2017

K Takanami; K Satoh; K Murata; T Sakamoto; H Sakamoto, The 9th World Congress of Itch, Morphological and molecular evolutional analyses of itch focused on the gastrin-releasing peptide system in mammals, Oct. 2017

Dec. 2023

Oct. 2023

Jul. 2019

Oct. 2018

Aug. 2018

Sep. 2017

Sep. 2017

Nov. 2016

Aug. 2014

Sep. 2012