KYOGOKU Daisuke
| Faculty Division of Natural Sciences Research Group of Biological Sciences | Associate Professor |
Last Updated :2025/11/27
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Profile Information
Name (Japanese)
KyogokuName (Kana)
Daisuke
Research Interests
Education
■Ⅱ．研究活動実績
Published Papers
- Cold Spring Harbor Laboratory, Field-crop transcriptome models are enhanced by measurements in systematically controlled environments, Yoichi Hashida; Daisuke Kyogoku; Suguru E Tanaka; Naoya Mori; Takanari Tanabata; Hiroyuki Watanabe; Atsushi J Nagano, Plants in the field respond to seasonal and diel changes in various environmental factors such as irradiance and temperature. We previously developed a statistical model that predicts rice gene expression from the meteorological data and identified the environmental factors regulating each gene. However, since irradiance and temperature (the two most critical environmental factors) are correlated in the field, it remains difficult to distinguish their roles in gene expression regulation. Here, we show that transcriptome dynamics in the field are predominantly regulated by irradiance, by the modelling involving diurnal transcriptome data from the 73 controlled conditions where irradiance and temperature were independently varied. The model's prediction performance is substantially high when trained using field and controlled conditions data. Our results highlight the utility of a systematic sampling approach under controlled environments to understand the mechanism of plant environmental response and to improve transcriptome prediction under field environments., 23 Sep. 2024, 10.1101/2024.09.21.614268
- Ecology Letters, Wiley, Evolution of realized niche breadth diversity driven by community dynamics, Daisuke Kyogoku, Abstract
Why many herbivorous insects are host plant specialists, with non‐negligible exceptions, is a conundrum of evolutionary biology, especially because the host plants are not necessarily optimal larval diets. Here, I present a novel model of host plant preference evolution of two insect species. Because habitat preference evolution is contingent upon demographic dynamics, I integrate the evolutionary framework with the modern coexistence theory. The results show that the two insect species can evolve into a habitat specialist and generalist, when they experience both negative and positive frequency‐dependent community dynamics. This happens because the joint action of positive and negative frequency dependence creates multiple (up to nine) eco‐evolutionary equilibria. Furthermore, initial condition dependence due to positive frequency dependence allows specialization to poor habitats. Thus, evolved habitat preferences do not necessarily correlate with the performances. The model provides explanations for counterintuitive empirical patterns and mechanistic interpretations for phenomenological models of niche breadth evolution., 21 Jan. 2024, 27, 1, Scientific journal, 10.1111/ele.14369 - Plant Biology, Wiley, Strong plastic responses in aerenchyma formation in
F1 hybrids of Imperata cylindrica under different soil moisture conditions, Y. Nomura; S. Arima; D. Kyogoku; T. Yamauchi; T. Tominaga, Abstract
Hybrids can express traits plastically, enabling them to occupy environments that differ from parental environments. However, there is insufficient evidence demonstrating how phenotypic plasticity in specific traits mediates hybrid performance. Two parental ecotypes of Imperata cylindrica produce F1 hybrids. The E‐type in wet habitats has larger internal aerenchyma than the C‐type in dry habitats. This study evaluated relationships between habitat utilisation, aerenchyma plasticity, and growth of I. cylindrica accessions. We hypothesize that plasticity in expressing parental traits explains hybrid establishment in habitats with various soil moisture conditions.
Aerenchyma formation was examined in the leaf midribs, rhizomes and roots of two parental ecotypes and their F1 hybrids in their natural habitats. In common garden experiments, we examined plastic aerenchyma formation in leaf midribs, rhizomes and roots of natural and artificial F1 hybrids and parental ecotypes and quantified vegetative growth performance.
In the natural habitats where soil moisture content varied widely, the F1 hybrids showed larger variation in aerenchyma formation in rhizomes than their parental ecotypes. In the common garden experiments, F1 hybrids showed high plasticity of aerenchyma formation in rhizomes, and their growth was similar to that of C‐type and E‐type under drained and flooded conditions, respectively.
The results demonstrate that F1 hybrids of I. cylindrica exhibit plasticity in aerenchyma development in response to varying local soil moisture content. This characteristic allows the hybrids to thrive in diverse soil moisture conditions., 08 Jan. 2024, Scientific journal, 10.1111/plb.13618 - PLOS ONE, Public Library of Science (PLoS), Male applicants are more likely to be awarded fellowships than female applicants: A case study of a Japanese national funding agency, Daisuke Kyogoku; Yoko Wada, Scientific grant applications are subjected to scholarly peer review. Studies show that the success rates of grant applications are often higher for male than for female applicants, suggesting that gender bias is common in peer review. However, these findings mostly come from studies in Europe, North America and Australia. Here we report the analyses of gender-specific success rates of applications to the fellowships offered by Japan Society for the Promotion of Science (JSPS). Because we analyze the observational data (i.e., not experimental), our aim here is to describe the possible gender gaps in the success rates, rather than the examination of gender bias per se. Results show that the success rates are consistently higher for male applicants than for female applicants among five different fellowship categories. The gender gaps in the success rates varied significantly between research fields in some Fellowship categories. Furthermore, in some fellowship categories, the gender gaps were significantly associated with the representation of female applicants (both positive and negative correlations were found). Though the causes of the gender gaps are unknown, unintentional gender bias during the review process is suggested. Pre-application gender gaps may also be contributing to the gender gaps in success rates. At least some of the observed gender gaps were relatively small, which may be partly explicable by the designs of the review process. However, gender gaps or biases acting prior to the application, such as self-selection bias, may have reduced the superficial gender gaps in the success rates. Further investigations that control for the effects of covariates (e.g., scientific merits of each applicant, which were not accessible to us) and those of other funding agencies, especially of non-Western countries, are warranted., 25 Oct. 2023, 18, 10, e0291372, e0291372, Scientific journal, 10.1371/journal.pone.0291372
- POPULATION ECOLOGY, Time series analysis showing how different environmental conditions affect the interspecific interactions of Callosobruchus maculatus and Callosobruchus chinensis, Takanori Kawase; Daisuke Kyogoku; Kazutaka Kawatsu; Noboru Katayama; Takeshi Miki; Michio Kondoh, Jun. 2023, Scientific journal, 10.1002/1438-390X.12160
- JOURNAL OF EVOLUTIONARY BIOLOGY, Female-limited responses in remating rate and mating duration in the experimental evolution of a beetle Callosobruchus chinensis, Daisuke Kyogoku; Shigeto Dobata; Rui Takashima; Teiji Sota, Jan. 2023, 36, 1, 309, 314, Scientific journal, 10.1111/jeb.14141
- TRENDS IN ECOLOGY & EVOLUTION, When Ecology Fails: How Reproductive Interactions Promote Species Coexistence, Miguel Gomez-Llano; Rachel M. Germain; Daisuke Kyogoku; Mark A. McPeek; Adam M. Siepielski, Jul. 2021, 36, 7, 610, 622, 10.1016/j.tree.2021.03.003
- ECOLOGICAL RESEARCH, Seed beetles as a modern model system of interspecific competition, Daisuke Kyogoku, Jul. 2021, 36, 4, 580, 589, Scientific journal, 10.1111/1440-1703.12244
- Refereed, Scientific Reports, Springer Science and Business Media LLC, Relationship between gene regulation network structure and prediction accuracy in high dimensional regression, Yuichi Okinaga; Daisuke Kyogoku; Satoshi Kondo; Atsushi J. Nagano; Kei Hirose,
Abstract The least absolute shrinkage and selection operator (lasso) and principal component regression (PCR) are popular methods of estimating traits from high-dimensional omics data, such as transcriptomes. The prediction accuracy of these estimation methods is highly dependent on the covariance structure, which is characterized by gene regulation networks. However, the manner in which the structure of a gene regulation network together with the sample size affects prediction accuracy has not yet been sufficiently investigated. In this study, Monte Carlo simulations are conducted to investigate the prediction accuracy for several network structures under various sample sizes. When the gene regulation network is a random graph, a sufficiently large number of observations are required to ensure good prediction accuracy with the lasso. The PCR provided poor prediction accuracy regardless of the sample size. However, a real gene regulation network is likely to exhibit a scale-free structure. In such cases, the simulation indicates that a relatively small number of observations, such as$$N=300$$
- JOURNAL OF EVOLUTIONARY BIOLOGY, Sexual selection increased offspring production via evolution of male and female traits, Daisuke Kyogoku; Teiji Sota, Mar. 2021, 34, 3, 501, 511, Scientific journal, 10.1111/jeb.13753
- WEED RESEARCH, Pollination from invasive dandelions is unlikely to reduce seed production in the native Japanese dandelion, Taraxacum japonicum, Daisuke Kyogoku, Dec. 2020, Scientific journal, 10.1111/wre.12462
- JOURNAL OF ANIMAL ECOLOGY, Species coexist more easily if reinforcement is based on habitat preferences than on species recognition, Daisuke Kyogoku; Hanna Kokko, Nov. 2020, 89, 11, 2605, 2616, Scientific journal, 10.1111/1365-2656.13321
- JOURNAL OF EVOLUTIONARY BIOLOGY, Heterospecific mating interactions as an interface between ecology and evolution, Daisuke Kyogoku; David Wheatcroft, Oct. 2020, 33, 10, 1330, 1344, 10.1111/jeb.13687
- TRENDS IN ECOLOGY & EVOLUTION, Intraspecific Adaptation Load: A Mechanism for Species Coexistence, Masato Yamamichi; Daisuke Kyogoku; Ryosuke Iritani; Kazuya Kobayashi; Yuma Takahashi; Kaori Tsurui-Sato; Akira Yamawo; Shigeto Dobata; Kazuki Tsuji; Michio Kondoh, Oct. 2020, 35, 10, 897, 907, 10.1016/j.tree.2020.05.011
- Refereed, POPULATION ECOLOGY, When does reproductive interference occur? Predictions and data, Daisuke Kyogoku, Apr. 2020, 62, 2, 196, 206, 10.1002/1438-390X.12041
- Refereed, GENES & GENETIC SYSTEMS, New microsatellite markers recognize differences in tandem repeats among four related Gastrodia species (Orchidaceae) (vol 94, pg 225, 2019), Kenji Ogaki; Kenji Suetsugu; Keiju Kishikawa; Daisuke Kyogoku; Kohtaroh Shutoh; Yuji Isagi; Shingo Kaneko, Feb. 2020, 95, 1, 52, 53, Scientific journal, 10.1266/ggs.95.52
- Refereed, Genes & genetic systems, Corrigendum: New microsatellite markers recognize differences in tandem repeats among four related Gastrodia species (Orchidaceae) [Genes Genet. Syst. (2019) 94, p. 225-229]., Kenji Ogaki; Kenji Suetsugu; Keiju Kishikawa; Daisuke Kyogoku; Kohtaroh Shutoh; Yuji Isagi; Shingo Kaneko, Tables 3 and 4 on p. 228 should be replaced with the corrected Tables 3 and 4 shown bellow., 2020, 95, 1, 52, 53, Scientific journal, False, 10.1266/ggs.95.52
- Refereed, GENES & GENETIC SYSTEMS, Development of microsatellite markers for the endangered sleeper Eleotris oxycephala (Perciformes: Eleotridae), Uchu Yamakawa; Shingo Kaneko; Ryosuke Imail; Leanne Kay Faulks; Koetsu Kon; Daisuke Kyogoku; Yuji Isagi; Yoshiaki Tsuda, Oct. 2019, 94, 5, 219, 224, Scientific journal, False, 10.1266/ggs.19-00007
- Refereed, EVOLUTIONARY ECOLOGY, Who determines the timing of inflorescence closure of a sexual dandelion? Pollen donors versus recipients, Daisuke Kyogoku; Yutaro Kataoka; Michio Kondoh, Oct. 2019, 33, 5, 701, 712, Scientific journal, 10.1007/s10682-019-10000-9
- Refereed, MAMMAL STUDY, Potential and pitfalls of the DNA metabarcoding analyses for the dietary study of the large Japanese wood mouse Apodemus speciosus on Seto Inland Sea islands, Jun J. Sato; Daisuke Kyogoku; Taketo Komura; Chiaki Inamori; Kouhei Maeda; Yasunori Yamaguchi; Yuji Isagi, Oct. 2019, 44, 4, 221, 231, Scientific journal, 10.3106/ms2018-0067
- Refereed, ECOLOGY AND EVOLUTION, Does past evolutionary history under different mating regimes influence the demographic dynamics of interspecific competition?, Daisuke Kyogoku; Michio Kondoh; Teiji Sota, Aug. 2019, 9, 15, 8616, 8624, Scientific journal, True, 10.1002/ece3.5397
- Refereed, GENES & GENETIC SYSTEMS, Development of microsatellite markers for an endangered fern in the Ryukyus, Plagiogyria koidzumii (Plagiogyriaceae), Kazuki Kurita; Daisuke Kyogoku; Atsushi Abe; Masatsugu Yokota; Yuji Isagi, Apr. 2019, 94, 2, 99, 102, Scientific journal, 10.1266/ggs.18-00056
- Refereed, GENES & GENETIC SYSTEMS, Development of microsatellite markers for the completely cleistogamous species Gastrodia takeshimensis (Orchidaceae) that are transferable to its chasmogamous sister G. nipponica, Keiju Kishikawa; Kenji Suetsugu; Daisuke Kyogoku; Kenji Ogaki; Daisuke Iga; Kohtaroh Shutoh; Yuji Isagi; Shingo Kaneko, Apr. 2019, 94, 2, 95, 98, Scientific journal, 10.1266/ggs.18-00057
- Refereed, EVOLUTIONARY ECOLOGY, Mating pattern of a distylous primrose in a natural population: unilateral outcrossing and asymmetric selfing between sexual morphs, Kurumi Arima; Daisuke Kyogoku; Naoyuki Nakahama; Kenji Suetsugu; Masato Ohtani; Chiyo Ishii; Hiroshi Terauchi; Yumiko Terauchi; Yuji Isagi, Feb. 2019, 33, 1, 55, 69, Scientific journal, 10.1007/s10682-018-9965-0
- Refereed, JOURNAL OF MAMMALOGY, Dietary niche partitioning between sympatric wood mouse species (Muridae: Apodemus) revealed by DNA meta-barcoding analysis, Jun J. Sato; Takuya Shimada; Daisuke Kyogoku; Taketo Komura; Shigeru Uemura; Takashi Saitoh; Yuji Isagi, Aug. 2018, 99, 4, 952, 964, Scientific journal, 10.1093/jmammal/gyy063
- Refereed, EVOLUTION, The evolution of between-species reproductive interference capability under different within-species mating regimes, Daisuke Kyogoku; Teiji Sota, Nov. 2017, 71, 11, 2721, 2727, Scientific journal, 10.1111/evo.13359
- Refereed, SCIENTIFIC REPORTS, A generalized population dynamics model for reproductive interference with absolute density dependence, Daisuke Kyogoku; Teiji Sota, May 2017, 7, 1, Scientific journal, 10.1038/s41598-017-02238-6
- Refereed, Conservation Genetics Resources, Development of microsatellite markers for an endemic shrub, Leptopetalum grayi (Rubiaceae), on the Ogasawara Islands using Ion Torrent next-generation sequencing, Tsuda Y; Nakahama N; Izuno A; Kyogoku D; Arima K; Kato H; Isagi Y, Dec. 2016, 8, 4, 587, 594, Scientific journal
- Refereed, JOURNAL OF EVOLUTIONARY BIOLOGY, Exaggerated male genitalia intensify interspecific reproductive interference by damaging heterospecific female genitalia, D. Kyogoku; T. Sota, Jun. 2015, 28, 6, 1283, 1289, Scientific journal, 10.1111/jeb.12646
- Refereed, POPULATION ECOLOGY, Reproductive interference: ecological and evolutionary consequences of interspecific promiscuity, Daisuke Kyogoku, Apr. 2015, 57, 2, 253, 260, 10.1007/s10144-015-0486-1
- Refereed, JOURNAL OF INSECT PHYSIOLOGY, Does heterospecific seminal fluid reduce fecundity in interspecific copulation between seed beetles?, Daisuke Kyogoku; Teiji Sota, Jan. 2015, 72, 54, 60, Scientific journal, 10.1016/j.jinsphys.2014.11.009
- Refereed, POPULATION ECOLOGY, The mechanism of the fecundity reduction in Callosobruchus maculatus caused by Callosobruchus chinensis males, Daisuke Kyogoku; Takayoshi Nishida, Jan. 2013, 55, 1, 87, 93, Scientific journal, 10.1007/s10144-012-0344-3
- Refereed, POPULATION ECOLOGY, The presence of heterospecific males causes an Allee effect, Daisuke Kyogoku; Takayoshi Nishida, Jul. 2012, 54, 3, 391, 395, Scientific journal, 10.1007/s10144-012-0313-x
MISC
- Not Refereed, GENES & GENETIC SYSTEMS, New microsatellite markers recognize differences in tandem repeats among four related Gastrodia species (Orchidaceae) (vol 94, pg 225, 2019), Kenji Ogaki; Kenji Suetsugu; Keiju Kishikawa; Daisuke Kyogoku; Kohtaroh Shutoh; Yuji Isagi; Shingo Kaneko, Feb. 2020, 95, 1, 52, 53, Others
- Not Refereed, GENES & GENETIC SYSTEMS, New microsatellite markers recognize differences in tandem repeats among four related Gastrodia species (Orchidaceae), Kenji Ogaki; Kenji Suetsugu; Keiju Kishikawa; Daisuke Kyogoku; Kohtaroh Shutoh; Yuji Isagi; Shingo Kaneko, Oct. 2019, 94, 5, 225, 229, False, 10.1266/ggs.19-00025
- Not Refereed, CONSERVATION GENETICS RESOURCES, Microsatellite records for volume 8, issue 4, Alejandra Paola Ojeda; Alexandre Wagner Silva Hilsdorf; Ana Caroline Leite; Ao-ao Yang; Ayako Izuno; Chongbo He; Chun-ya Zhou; Daisuke Kyogoku; Danilo Caneppele; Dongmei Zhu; Fernando Stopato da Fonseca; Hao Yin; Hidetoshi Kato; Jackeline Alves Vilar; Jiale Li; Jianqing Peng; Kurumi Arima; Ling Zhu; L. V. Yefeng; Meilin Tian; Mingyu Di; Naoyuki Nakahama; Shilei Li; Weimin Wang; Wen Song; Weidong Liu; Xiaojun Liu; Xuekai Han; Ying Pan; Yong-quan Su; Yuichi Tsuda; Yuji Isagi; Yunfeng Li; Zhi-meng Zhuang; Zhiyi Bai; Zunchun Zhou, Dec. 2016, 8, 4, 587, 594, Others, 10.1007/s12686-016-0635-7
- Not Refereed, JAPANESE JOURNAL OF ECOLOGY, The Ecological Society of Japan, The role of adaptive interspecific promiscuity in the history of ecological research(
Reproductive interference and ecological communities), Honma Atsushi; Kishi Shigeki; Suzuki Noriyuki; Kyogoku Daisuke, 2012, 62, 2, 217, 224, 10.18960/seitai.62.2_217 - Not Refereed, JAPANESE JOURNAL OF ECOLOGY, The Ecological Society of Japan, Present knowledge and future directions in understanding the role of frequency dependence in reproductive interference(
Reproductive interference and ecological communities), Kyogoku Daisuke; Nishida Takayoshi, 近縁種間にしばしば認められる排他的な関係は長らく資源競争によってもたらされると考えられてきたが、多くの実証研究が行われたにもかかわらず、資源競争の重要性が示されたとは言いがたい。これに対し、繁殖干渉と呼ばれる配偶過程において生じる誤った種間での求愛行動などが、近縁種の関係を決定する上で重要であると考えられるようになってきた。繁殖干渉は相対的に個体数の多い種のほうが相手種に対してより強い悪影響を与える、すなわち頻度依存的にはたらくと考えられてきた。ここで本稿では繁殖干渉の強さを、繁殖干渉による個体の適応度の低下量と定義する。繁殖干渉はその頻度依存性によって正のフィードバックを生じやすく、そのため近縁種間の排他的な関係を説明する論理として資源競争よりも一般性が高いと考えられてきた。しかし相互作用する2種の総密度が大きく変化しても2種の頻度だけで繁殖干渉の強さを十分説明できるのかはこれまでほとんど問題にされてこなかった。実際には、総密度の変化が2種の頻度と繁殖干渉の強さの関係を変化させる可能性がある。また、配偶者選択のような行動が2種の頻度に応じて変化する場合には、繁殖干渉のはたらき方がこれまで考えられてきたよりも複雑になる可能性がある。個体が経験する繁殖干渉の強さは同所的に存在する同種個体・他種個体双方の個体数によって決まるため、繁殖干渉を考える上で個体群生態学的な視点は欠かせないが、我々の繁殖干渉に対する個体群生態学的な理解はあまり進んではいない。繁殖干渉がもたらす種々の生態学的・進化的な帰結を統一的に理解するためにも、繁殖干渉の個体群生態学的な研究が必要である。, 2012, 62, 2, 239, 245, 10.18960/seitai.62.2_239
Awards
Research Projects
- Grant-in-Aid for Scientific Research (B), 01 Apr. 2022 - 31 Mar. 2027, 23K23949, Mechanism of parallel and allopatric speciation in a shell-brooding cichlid, 高橋 鉄美; 京極 大助; 二階堂 雅人; 渡邉 正勝, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, University of Hyogo, 17030000, 13100000, 3930000, タンガニイカ湖に固有なTelmatochromis temporalisには三つの生態型が見られる。岩の隙間に隠れる普通型（最大体長８cm）、シェルベッド（巻貝の空き殻が敷き詰められた底質）に生息して貝殻を隠れ場所とする矮小型（同４cm）、そして岩と砂の混ざった底質に生息して岩の下に隠れる痩身型（同６cm）である。本研究では、矮小型の進化に注目して、１）普通型の体サイズに関するstanding genetic variation (SGV)が矮小型への平行進化を可能にしたとする仮説を分子生物学的に検証し、２）側所的種分化の初期段階として、岩場とシェルベッドで体サイズに対する同類交配が生じるかを飼育実験で確かめる。
１の研究では、体サイズの責任遺伝子を探索するため、pooled genome-wide association study (pool-GWAS)を行った。その結果、矮小型が並行して進化したチブウェンソロとウォンジにおいて、１０個の共通する一塩基多型（SNPs）を特定した。さらにそれらのSNPsを、普通型と矮小型を交配して作成したF2でジェノタイピングし、３つのSNPsで体サイズと関係することが明らかとなった。これらのSNPsは、普通型で多型として維持されており（SGV）、シェルベッドが出現した際に、小さい体サイズを発現するアリルに選択圧がかかり、矮小型が進化したことを示している。しかし、これらのSNPsでは体サイズに対する寄与率が低いため、他の遺伝子も体サイズ進化に関わっていると考えられ、今後の課題として残った。
２の研究では、両型を交配させたF2が必要である。現在、昨年３月に持ち帰った個体からF1を20個体程度得たところである。まだ成熟していないが、飼育は順調に行われている。
このほか、現地調査で痩身型の進化について調べた。それを含め、３報を投稿中である。, kaken - Grant-in-Aid for Scientific Research (B), 01 Apr. 2022 - 31 Mar. 2027, 22H02686, Mechanism of parallel evolution and allopatric speciation of dwarf morph that uses empty shells, 高橋 鉄美; 京極 大助; 二階堂 雅人; 渡邉 正勝, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), University of Hyogo, 17030000, 13100000, 3930000, kaken
- 2024 - 2027, Principal investigator, 花寿命をめぐる花粉とめしべの敵対的な相互作用とその進化, 京極 大助, 科学技術振興機構, 戦略的な研究開発の推進 戦略的創造研究推進事業 さきがけ, 兵庫県立人と自然の博物館, 進化生態学の理論予測にもとづき「花粉は花を枯死させる性質を進化させ、めしべはこれに抵抗する性質を進化させる」という仮説を多角的なアプローチで検証します。主にシロイヌナズナを用いて、花粉とめしべの性質を決定している遺伝子を特定します。また、近縁種間での遺伝子の系統進化パターン等を明らかにします。これらにより、ミクロスケールからマクロスケールを統合しつつ、花粉とめしべの「生命力」を明らかにします。, j_global
- Grant-in-Aid for Scientific Research (B), 01 Apr. 2022 - 31 Mar. 2026, 23K23969, Pollen-pistil interactions over floral longevity, 京極 大助; 高橋 鉄美; 藤井 壮太, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, The Museum of Nature and Human Activities, Hyogo, 16900000, 13000000, 3900000, シロイヌナズナ約220アクセッションの花粉を標準系統（Sha）のめしべに受粉し、乳頭細胞が枯死するまでの時間を計測することで、ゲノムワイド関連解析用の表現型データを取得した。現在までにおおむね700程度のサンプルサイズを得ている（枯死の開始と終了など複数形質を測定しているため、サンプルサイズは形質ごとに多少異なる）。公開されているリシーケンスデータを処理するパイプラインを構築し、得られた表現型と相関するSNPを探索した。遺伝子発現情報も参考に、いくつかの候補遺伝子を選定した。また、今後用いるためのバイオインフォマティクス解析用のパイプライン構築がほぼ完了した。, kaken
- Grant-in-Aid for Scientific Research (B), 01 Apr. 2022 - 31 Mar. 2026, 22H02706, Pollen-pistil interactions over floral longevity, 京極 大助; 藤井 壮太; 高橋 鉄美, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), The Museum of Nature and Human Activities, Hyogo, 16900000, 13000000, 3900000, kaken
- Grant-in-Aid for Early-Career Scientists, 01 Apr. 2019 - 31 Mar. 2022, 19K16222, A novel model of realized niche evolution, Kyogoku Daisuke, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 4160000, 3200000, 960000, Resource utilization patterns of herbivorous insects are often inexplicable by their physiological constraints. By simulation modeling, this study showed that hybridization (or other forms of costly interspecific mating interactions) between closely related species can limit the diversity of resources they use, as a result of the evolution of habitat (e.g. host plant) preferences to avoid interspecific encounters. A literature survey was also carried out to examine the biological plausibility of a model assumption (the degree of physiological constraints). In addition, mathematical formulae to analyze the data compiled from the literature were developed., kaken
- Apr. 2021 - Mar. 2022, 外来タンポポが在来タンポポを駆逐する機構の解明, 市村清新技術財団, 植物研究助成, 兵庫県立人と自然の博物館
- 特別研究員奨励費, 22 Apr. 2016 - 31 Mar. 2019, 16J03061, 繁殖形質置換の生じやすさに影響する要因：理論と実証, 京極 大助, 日本学術振興会, 科学研究費助成事業 特別研究員奨励費, 4420000, 3400000, 1020000, まず2018年度には、外来タンポポ（雑種含む）の花粉が在来種カンサイタンポポの種子生産に与える影響をハウス実験によって調べた。外来タンポポにはセイヨウタンポポ、アカミタンポポ、これらと在来種との雑種（複数の倍数体を含む）があるが、これらの素性をすべて明らかにしたうえで特定の外来タンポポが在来種の種子生産に影響している可能性を検討した。実験の結果、いずれの外来タンポポの花粉も在来タンポポの種子生産を低下させなかった。この結果は外来タンポポの花粉が在来タンポポの種子生産を低下させるとした先行研究と矛盾するものであり、外来タンポポと在来タンポポの相互作用メカニズムについて再考を迫るものである。本実験の結果はまもなく論文として専門誌に投稿予定である。
また2018年度には、カンサイタンポポの繁殖生態に関する論文の執筆を行った。前年度までに、カンサイタンポポの頭花が受粉によって閉じることが明らかとなった。この結果を論文としてまとめ、専門誌に投稿した。現在論文は大幅改定の支持を受けており、まもなく受理されるものと期待される。
加えて、2018年度には数理モデルの結果をまとめた論文の執筆を行った。前年度までに、繁殖干渉をさける適応として棲み分けと種認識の相対的な進化のしやすさを数理モデルにより検討し、棲み分けの方が生じやすいこと、両形質が同時に進化できる場合には住み分けが種認識の進化を促進するがその逆は生じないことなどが明らかになっていた。論文はいくつかの雑誌に掲載を拒否されているが、近いうちにいずれかの雑誌に受理されるものと期待される。, kaken - Grant-in-Aid for Scientific Research (B), 01 Apr. 2016 - 31 Mar. 2019, 16H04846, Ecology of Adaptation Load: The Effect of Adaptation to Interspecific Competition to Multi-Species Coexistence, Kondoh Michio, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), 17420000, 13400000, 4020000, For better understanding of how evolution of traits related to interspecific interactions, such as sexual selection, mate competition, social or cooperative behavior, sexual reproduction, affected community dynamics and structure, we took three approaches based on review, theoretical and empirical research. Our theory suggests that adaptation driven by interspecific mate competition or sexual conflict may enhance multi-species coexistence by strengthening self-regulation intensities of populations or weakening interspecific competition. Indeed, some earlier reports can be viewed as special cases of our more general theory. Empirical studies using bean beetles or fish also gave evidence that traits linked to intraspecific relationship affect interspecific interactions and community dynamics,, url
- 特別研究員奨励費, 01 Apr. 2013 - 31 Mar. 2015, 13J01115, 性的対立による形質進化がもたらす繁殖干渉の非対称性, 京極 大助, 日本学術振興会, 科学研究費助成事業 特別研究員奨励費, 京都大学, 2000000, 2000000, アズキゾウムシのオスは種間交尾を介してヨツモンマメゾウムシの産卵数を低下させる。しかし種間交尾が実際にどのように産卵数に影響するのかは不明であった。種間交尾によって雑種ができないことから、射精物の生理活性と生殖器の物理的損傷が産卵数低下の原因として考えられたが、前年度までの実験から射精物の効果は無視できることが分かっている。アズキゾウムシのオス交尾器には棘があり、この棘がヨツモンマメゾウムシのメス生殖器を傷つけることで産卵数低下を引き起こしている可能性が考えられた。そこで交尾器形態のさまざまに異なるアズキゾウムシのオスを用いることで交尾器形態がヨツモンマメゾウムシの産卵数に与える影響を調べた。この実験は前年度までに行っていたので、今年度は実験結果の詳細に解析を行った。その結果以下の事が明らかとなった。(i)より発達した交尾器の棘を有するアズキゾウムシのオスほど、種間交尾を介してヨツモンマメゾウムシのメス生殖器を傷つけやすかった。また(ii)種間交尾による生殖器の物理的損傷は、ヨツモンマメゾウムシの産卵数を低下させた。先行研究から、アズキゾウムシを含むマメゾウムシ類のオス交尾器は、性選択の一種である種内でのオス同士の受精をめぐる競争（精子競争）により発達してきたと考えられている。したがって本研究の結果は、種内での性選択の結果発達してきたオスの加害形質が種間相互作用にも影響しうることを示している。本研究はオスの加害形質が種間相互作用に影響しうることを示した点で高い新規性を有する。, kaken
■Ⅲ．社会連携活動実績
１．公的団体の委員等（審議会、国家試験委員、他大学評価委員，科研費審査委員等）
