研究者総覧

坂口 修一 (サカグチ シュウイチ)

  • 研究院自然科学系生物科学領域 准教授
メールアドレス:
guchicc.nara-wu.ac.jp
Last Updated :2021/06/02

researchmap

学位

  • 理学博士, 東京大学
  • 理学修士, 東京大学

研究キーワード

  • 高等植物 発生学 形態形成 茎頂 葉序 微小管 情報伝達 クローン解析 遺伝子組換え 組織培養 植物の運動 電子顕微鏡 マイクロX線CT 

研究分野

  • ライフサイエンス, 形態、構造

経歴

  • 2012年04月 奈良女子大学研究院自然科学系生物科学領域准教授
  • 2003年04月 - 2012年03月 奈良女子大学理学部生物科学科助教授
  • 1999年04月 - 2003年03月 奈良女子大学大学院人間文化研究科人間環境科学専攻生命環境講座助教授
  • 1994年04月 - 1999年03月 奈良女子大学理学部生物科学科助教授
  • 1990年09月 - 1994年03月 奈良女子大学理学部生物科学科助手
  • 1988年11月 - 1990年08月 岡崎国立共同研究機構基礎生物学研究所博士研究員

学歴

  • - 1988年 東京大学 理学系研究科 植物学
  • - 1982年 東京大学 理学部 生物学(植物学)

委員歴

  • 2013年01月 - 2016年12月 日本植物学会 編集委員(editor: Journal of Plant Research) society

    学協会

  • 2010年01月 - 2013年12月 日本植物形態学会 評議員 society

    学協会

  • 2011年01月 - 2012年12月 日本植物学会 編集委員会委員(Editorial board member: Journal of Plant Research) society

    学協会

  • 2010年01月 - 2011年12月 日本植物形態学会 広報委員(庶務幹事兼任) society

    学協会

  • 2008年01月 - 2011年12月 日本植物形態学会 庶務幹事 society

    学協会

  • 2011年04月 - 2011年09月 日本植物形態学会 第23回大会準備委員長 society

    学協会

  • 2010年04月 - 2010年09月 日本植物形態学会 第22回大会準備委員長 society

    学協会

  • 2009年04月 - 2009年09月 日本植物形態学会 第21回大会準備委員長 society

    学協会

  • 2008年04月 - 2008年09月 日本植物形態学会 第20回大会準備委員長 society

    学協会

  • 2006年01月 - 2007年12月 日本植物生理学会 選挙管理委員 society

    学協会

  • 1998年04月 - 2004年03月 日本植物形態学会 編集委員 society

    学協会

  • 2000年01月 - 2001年12月 日本植物形態学会 評議員 society

    学協会

論文

  • Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley

    Makiko Nagai; Miwa Ohnishi; Takeo Uehara; Mutsumi Yamagami; Eiko Miura; Mai Kamakura; Akira Kitamura; Shu-Ichi Sakaguchi; Wataru Sakamoto; Teruo Shimmen; Hidehiro Fukaki; Robert J. Reid; Akio Furukawa; Tetsuro Mimura

    The concentration of ions in plant cells and tissues is an essential factor in determining physiological function. In the present study, we established that concentration gradients of mobile ions exist in both xylem exudates and tissues within a barley (Hordeum vulgare) primary leaf. For K+ and NO3-, ion concentrations generally decreased from the leaf base to the tip in both xylem exudates and tissues. Ion gradients were also found for Pi and Cl- in the xylem. The hydathode strongly absorbed Pi and re-translocated it to the rest of the plant, whereas Cl- was extruded. The ion concentration gradients developed early during leaf growth, increased as the tissue aged and remained under both high and low transpiration conditions. Measurement of the expression profiles of Pi, K+ and NO3- transporters along the longitudinal axis of the leaf revealed that some transporters are more expressed at the hydathode, but for most transporters, there was no significant variation along the leaf. The mechanisms by which longitudinal ion gradients develop in leaves and their physiological functions are discussed. © 2013 John Wiley & Sons Ltd., 2013年10月, Plant, Cell and Environment, 36 (10), 1826 - 1837, doi;pubmed

    研究論文(学術雑誌)

  • Unique profiles of changes in cell membrane fluidity during ethanol-induced yeast to pseudohyphal transition in Candida tropicalis.

    坂口修一; Suzuki, T; Kono, K; Tawara, S; Fujimura, T; Ito, T; Omi, K; Ohbuchi, K; Komatsu, Y; Sakaguchi, S; Kamihara, T

    石油資化酵母Candida tropicalisのエタノール添加培養では、膜の流動性が上昇して脱極性化した細胞となり、その後に膜の流動性が戻る過程で菌糸形成が起こることを、DPHを蛍光プローブとした蛍光偏光法によって解析し、あわせて膜のリン脂質成分変化量との対応関係を調べた。, 2010年, Journal of General and Applied Microbiology, 56, 321-329

  • Somaclonal variation and the ploidy Level in Phalaenopsis alliance.

    坂口修一; 周天甦

    2003年, Plant Morphology, 15 (1), 2-7

  • Development and disintegration of phragmoplasts in living cultured cells of a GFP :: TUA6 transgenic Arabidopsis thaliana plant

    K Ueda; S Sakaguchi; F Kumagai; S Hasezawa; H Quader; U Kristen

    Cultured suspension cells of Arabidopsis thaliana that stably express a green-fluorescent protein-alpha-tubulin 6 fusion protein were used to follow the development and disintegration of phragmoplasts. The development and disintegration of phragmoplasts in the living cultured cells could be successively observed by detecting the green-fluorescent protein fluorescence of the microtubules. In the early telophase spindle, where two kinetochore groups and two daughter chromosome groups had completely separated from one another, fluorescence appeared in the interzone between the two chromosome groups. The fluorescent region was gradually condensed at the previous equator and increased in fluorescence intensity, and finally it formed the initial phragmoplast. The initial phragmoplast moved from the cell center towards the cell periphery, and it lost fluorescence at its center and became double rings in shape. The expansion orientation of the phragmoplast was not always the same as that of the future new cell wall before it came in contact with the cell wall. The phragmoplast did not usually come in contact with the cell wall simultaneously with its entire length. A portion of the phragmoplast which was earlier in contact with the cell wall disappeared earlier than other portions of the phragmoplast. The duration of contact between any portions of the phragmoplast and the plasma membrane of the cell wall was 15-30 min. The fluorescence intensity of the cytoplasm did not seem to be elevated by the disintegration of the strongly fluorescent phragmoplast., 2003年, PROTOPLASMA, 220 (3-4), 111 - 118, doi;web_of_science

    研究論文(学術雑誌)

  • ナツミカンCitrus natsudaidai Hayata果実の毛状体の起源と発生.

    坂口修一; 杉山明子

    2002年, Plant Morphology, 14 (1), 29-33

  • Ca2+ signal is generated only once in the mating pheromone response pathway in Saccharomyces cerevisiae

    J Nakajima-Shimada; S Sakaguchi; F Tsuji; Y Anraku; H Iida

    The mating pheromone, alpha-factor, of the yeast Saccharomyces cerevisiae binds to the heterotrimeric G protein-coupled cell surface receptor of MATa cells and induces cellular responses necessary for mating. In higher eukaryotic cells, many hormones and growth factors rapidly mobilize a second messenger, Ca2+, by means of receptor-C protein signaling. Although striking similarities between the mechanisms of the receptor-G protein signaling in yeast and higher eukaryotes have long been known, it is still uncertain whether the pheromone rapidly mobilizes Ca2+ necessary for early events of the pheromone response. Here we reexamine this problem using. sensitive methods for detecting Ca2+ fluxes and mobilization, and find no evidence that there is rapid Ca2+ influx leading to a rapid increase in the cytosolic free Ca2+ concentration. In addition, the yeast PLC1 deletion mutant lacking phosphoinositide-specific phospholipase C, a hey enzyme for generating Ca2+ signals in higher eukaryotic cells, responds normally to the pheromone. These findings suggest that the receptor-G protein signaling does not utilize Ca2+ as a second messenger In the early stage of the pheromone response pathway, Since the receptor-G protein signaling does stimulate Ca2+ influx after early events have finished and this stimulation is essential for late events in the pheromone response pathway {Iida et al,, (1990) J, Biol, Chem., 265: 13391-13399} Ca2+ may be used only once in the signal transduction pathway in unicellular eukaryotes such as yeast., 2000年04月, CELL STRUCTURE AND FUNCTION, 25 (2), 125 - 131, web_of_science

    研究論文(学術雑誌)

  • Roles of Ca2+ in hyphal and yeast-form growth in Candida albicans. Growth regulation by altered extracellular and intracellular free Ca2+ concentrations.

    坂口修一; 渋谷恭子他

    1997年, Mycoscience, 38, 215-225

  • Overproduction of Cdc24p (Cls4p), a guanine nucleotide-exchange factor toward Cdc42 GTPase, impairs initiation of budding in Saccharomyces cerevisiae

    S Sakaguchi; S Miyamoto; H Iida; T Suzuki; Y Ohya; Y Anraku

    An entire coding region of the CDC24/CLS4 gene and its truncated derivatives were overexpressed in yeast cells under the control of the GAL1 promoter. Western blotting analysis of the yeast cell lysates showed that the CDC24/CLS4 protein (Cdc24p) was induced to reach its maximum level after 9 h incubation of the cells in galactose medium. Overexpression of Cdc24p within the cells caused the morphological change, accumulating large spherical unbudded cells which exhibited actin cytoskeleton disturbed, chitin delocalized on the cell surface, and cell viability decreased. Multiple nuclei were observed in these cells, indicating that only budding cycle bur not nuclear division cycle is blocked by the overproduction of Cdc24p. In order to identify the region of Cdc24p responsible for the growth inhibition, several truncated CDC24 genes were expressed. Surprisingly, overexpression of fragments either containing the C-terminal 76 amino acid residues or deleting the same region inhibited cellular growth. This suggests that Cdc24p contains multiple functional domains for its tasks, likely cooperating signals of bud positioning and bud timing., 1995年, PROTOPLASMA, 189 (3-4), 142 - 148, web_of_science

    研究論文(学術雑誌)

  • Overproduction of Cdc24p(cls4p), a GTP-GDP exchange factor toward Cdc42 GTPase impairs initiation of budding in Saccharomyces cerevisiae.

    坂口修一; 大矢禎一他

    1995年, Protoplasma, 189 (3-4), 142-148

  • OCCURRENCE OF CHROMOSOME REARRANGEMENTS DURING THE FUSION PROCESS IN THE IMPERFECT YEAST CANDIDA-ALBICANS

    T SUZUKI; M YAMADA; S SAKAGUCHI

    Auxotrophic derivatives of three strains of the pathogenic yeast Candida albicans of different origins, including 1006 derived from CBS5736, A5153 derived from FC18 and NARA2 derived from NUM961, were used in spheroplast fusion experiments. The DNA content of the prototrophic fusion product obtained following fusion between strains 1006 and A5153 approximated to the sum of those of the parents, but was variable when NARA2 was used as the parent for fusion. Chromosome-sized DNA molecules of the fusion derivatives were separated by pulsed-field gel electrophoresis to examine whether either or both of the chromosome-sized DNA molecules of each parent were transferred into the fusion derivatives. In the fusion derivatives obtained following fusion between strains 1006 and A5153, nearly the full complement of chromosomes was shown to be transferred, but partial transfer of chromosomes occurred in the fusion derivatives that were obtained following fusion between strains NARA2 and A5153. Results indicated that chromosome loss also occurred when these two strains were fused. Variations in the size of R chromosomes, the rDNA-containing chromosomes, were observed in all fusion derivatives tested, indicating high-frequency recombination between R chromosomes during the fusion process., 1994年12月, MICROBIOLOGY-UK, 140, 3319 - 3328, web_of_science

    研究論文(学術雑誌)

  • CORRELATION BETWEEN POLYPLOIDY AND AUXOTROPHIC SEGREGATION IN THE IMPERFECT YEAST CANDIDA-ALBICANS

    T SUZUKI; A HITOMI; PT MAGEE; S SAKAGUCHI

    In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperature-dependent or -independent manner, when incubated at low (28 degrees C) and high (37 degrees C) temperatures. In the case of the temperature-dependent type of variants, high-ploidy cells appeared at 37 degrees C but rarely at 28 degrees C. This phenotype was named Pld(ts) (temperature-sensitive polyploidization), and the temperature-independent phenotype was called Pld(-). The appearance of high-ploidy cells in the culture of the Pld(ts) strain at 37 degrees C was accompanied by a significant increase in the frequency of auxotrophic variants; these variants probably occur as a result of segregation of auxotrophic markers from the heterozygous to the homozygous state. Both Pld(ts) and Pld(-) phenotypes were recessive in a fusion with a Pld(+) parent. An adenine auxotrophic marker (ade1) was introduced into a Pld(ts) strain in a heterozygous state, and the individual high-ploidy cells of this strain, grown at 37 degrees C, were micromanipulated to form colonies, which consisted of red and white sectors appearing at high frequency on a pink background. When the ade1 auxotrophy was introduced into Pld(-) strains, frequently sectored colonies were produced. These results suggested an increased level of chromosome missegregation in both types of Pld mutants. Analyses by pulsed-field gel electrophoresis of Ade(-) segregants, derived from a micromanipulated high-ploidy cell of a Pld(ts) strain, suggested the occurrence of nonreciprocal recombination, some of which includes chromosome loss., 1994年06月, JOURNAL OF BACTERIOLOGY, 176 (11), 3345 - 3353, web_of_science

    研究論文(学術雑誌)

  • A DBL-homologous region of the yeast CLS4/CDC24 gene product is important for Ca2+-modulated bud Assembly.

    坂口修一; 宮本茂美他

    1991年, Biochemical and Biophysical Research Communications, 181, 604-610

  • CELL-CYCLE CONTROL BY CA-2+ IN SACCHAROMYCES-CEREVISIAE

    H IIDA; S SAKAGUCHI; Y YAGAWA; Y ANRAKU

    1990年12月, JOURNAL OF BIOLOGICAL CHEMISTRY, 265 (34), 21216 - 21222, web_of_science

    研究論文(学術雑誌)

  • SPECIFIC ARRANGEMENTS OF CORTICAL MICROTUBULES ARE CORRELATED WITH THE ARCHITECTURE OF MERISTEMS IN SHOOT APICES OF ANGIOSPERMS AND GYMNOSPERMS

    S SAKAGUCHI; T HOGETSU; N HARA

    1990年06月, BOTANICAL MAGAZINE-TOKYO, 103 (1070), 143 - 163, web_of_science

    研究論文(学術雑誌)

  • ARRANGEMENT OF CORTICAL MICROTUBULES AT THE SURFACE OF THE SHOOT APEX IN VINCA-MAJOR L - OBSERVATIONS BY IMMUNOFLUORESCENCE MICROSCOPY

    S SAKAGUCHI; T HOGETSU; N HARA

    1988年12月, BOTANICAL MAGAZINE-TOKYO, 101 (1064), 497 - 507, web_of_science

    研究論文(学術雑誌)

  • ARRANGEMENT OF CORTICAL MICROTUBULES IN THE SHOOT APEX OF VINCA-MAJOR L - OBSERVATIONS BY IMMUNOFLUORESCENCE MICROSCOPY

    S SAKAGUCHI; T HOGETSU; N HARA

    1988年09月, PLANTA, 175 (3), 403 - 411, web_of_science

    研究論文(学術雑誌)

  • INTRA-LEAF AND INTRACELLULAR GRADIENTS IN CHLOROPLAST ULTRASTRUCTURE OF DORSIVENTRAL LEAVES ILLUMINATED FROM THE ADAXIAL OR ABAXIAL SIDE DURING THEIR DEVELOPMENT

    TERASHIMA, I; S SAKAGUCHI; N HARA

    1986年09月, PLANT AND CELL PHYSIOLOGY, 27 (6), 1023 - 1031, web_of_science

    研究論文(学術雑誌)

MISC

  • 高等学校理科「生物基礎」における生態学教育 ?猿沢池のアオコを題材とした実践的授業展開とその高大連携的検討 ?

    矢野幸洋; 鍵和田聡; 坂口修一; 吉川尚男

    2017年10月, 教育システム研究(奈良女子大学教育システム研究開発センター)別冊「本学の教員養成課程の改善・高度化に向けた大学教員と附属教員の連携研究推進事業」成果論文集, 別冊, 147-151

  • 植物器官表面の細胞観察法

    坂口修一

    海外で開発された最新技術を紹介するとともに日本でおこなうための改変点について記載した。, 1995年, Plant Morphology, 7 (1), 59-64

書籍等出版物

  • 植物学の百科事典

    坂口修一; 相田光宏 (, 範囲: 分担)

    丸善出版, 2016年06月, 536-539, 542-543

  • Atlas of Plant Cell Structure

    坂口修一; Tetsuko Noguchi (, 範囲: 分担)

    Springer, 2014年09月, 134-135 (ISBN: 9784431549406)

  • 岩波生物学辞典、第五版

    坂口修一; 相羽惠介 (, 範囲: 分担)

    岩波書店, 2013年02月, 63--1476中の56ヵ所

  • 生物学辞典

    坂口修一; 相川真一 (, 範囲: 分担)

    東京化学同人, 2010年12月, p. 337他、71カ所

  • これでナットク!植物の謎 植木屋さんも知らないたくましいその生き方\n(ブルーバックスB-1565)

    坂口修一; 浅田浩二 (, 範囲: その他)

    講談社, 2007年08月, 71-73, 73-78, 103-106

  • 食Up to Date - 食と健康|食と安全|食と環境 -

    坂口修一; 松田覚他 (, 範囲: 分担)

    金芳堂、京都, 2005年01月, pp. 194-211

講演・口頭発表等

  • コチョウラン花柄のねじれ運動機構解明にむけた阻害剤処理実験系の検討

    福田有希; 坂口修一

    日本植物学会第76回大会, 2012年09月, 日本植物学会, false

  • コチョウラン花柄のねじれ運動と微小管との関係について

    福田有希; 坂口修一

    日本植物形態学会, 2011年09月, 東京(日本女子大学), false

  • タバコ葉にみられる潜在的異形葉性の観察

    齋藤幸恵; 坂口修一

    日本植物形態学会, 2011年09月, 東京(日本女子大学), false

担当経験のある科目(授業)

  • 生物学実験(C) (奈良女子大学)

  • 生物学実験(B) (奈良女子大学)

  • 生物学実験(A) (奈良女子大学)

  • 実践生物環境科学演習Ⅰ (奈良女子大学)

  • 分子・細胞生物学特論1 (奈良女子大学)

  • 分子・細胞生物学特論8 (奈良女子大学)

  • 分子細胞工学実習 (奈良女子大学)

  • 卒業研究III (奈良女子大学)

  • 卒業研究IV (奈良女子大学)

  • 生物形態発生学実習 (奈良女子大学)

  • 細胞調節学セミナーⅣ (奈良女子大学)

  • 細胞調節学セミナーⅡ (奈良女子大学)

  • 生物科学演習Ⅰ (奈良女子大学)

  • サイエンス・オープンラボⅠ(D) (奈良女子大学)

  • 生物科学特論17 (奈良女子大学)

  • 生物科学演習I (奈良女子大学)

  • 生物学実験I (奈良女子大学)

  • 生物学特別講義III(D) (奈良女子大学)

  • 生物科学英語II (奈良女子大学)

  • 基礎生物学1 (奈良女子大学)

  • 生物科学英語III (奈良女子大学)

  • 展開実習 (奈良女子大学)

  • 環境 (奈良女子大学)

  • 生物学特別講義V (奈良女子大学)

  • 基礎科学英語 (奈良女子大学)

  • 植物生理学実習 (奈良女子大学)

  • 細胞調節学特論Ⅱ (奈良女子大学)

  • 植物形態学 (奈良女子大学)

  • 植物形態分類学実習 (奈良女子大学)

  • 基礎生物学実習II (奈良女子大学)

  • 基礎生物学実習I (奈良女子大学)

  • 細胞調節学セミナーIV (奈良女子大学)

  • 生物科学方法論 (奈良女子大学)

  • 生物学特別講義XIII (奈良女子大学)

  • 植物形態形成論演習 (奈良女子大学)

  • 植物形態形成論 (奈良女子大学)

  • 植物形態形成調節論演習 (奈良女子大学)

  • 植物形態形成調節論 (奈良女子大学)

  • 生物科学特別研究IV (奈良女子大学)

  • 生物科学特別研究II (奈良女子大学)

  • 卒業研究II (奈良女子大学)

  • 細胞生理学実習 (奈良女子大学)

  • 形態学実習 (奈良女子大学)

  • 分子・細胞生物学実習 (奈良女子大学)

  • 形態学 (奈良女子大学)

  • 生物科学2 (奈良女子大学)

  • 細胞調節学特論II (奈良女子大学)

  • 生物科学特別研究III (奈良女子大学)

  • 生物科学特別研究I (奈良女子大学)

  • 卒業研究I (奈良女子大学)

  • 植物分類学実習 (奈良女子大学)

  • 植物分類学 (奈良女子大学)

  • 細胞調節学セミナーII (奈良女子大学)

  • 細胞生理学 (奈良女子大学)

所属学協会

  • American Sciety of Plant Biologists

  • Botanical Sciety of America

  • 日本植物生理学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物形態学会

  • 日本植物学会

  • 日本植物学会



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