研究者総覧

安田 恵子 (ヤスダ ケイコ)

  • 副学長 副学長
  • 研究院自然科学系生物科学領域 教授
  • 男女共同参画推進機構 男女共同参画推進機構長
メールアドレス:
ponkocc.nara-wu.ac.jp
Last Updated :2021/07/07

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学位

  • 博士(医学), 京都大学
  • 修士(理学), 奈良女子大学

研究キーワード

  • 生殖内分泌 卵巣 精巣 

研究分野

  • ライフサイエンス, 動物生理化学、生理学、行動学

経歴

  • 2016年01月 教授
  • 2013年04月 - 2015年12月 准教授
  • 1996年04月 - 2013年03月 講師
  • 1994年04月 - 1996年03月 助手
  • 2020年04月 奈良女子大学 副学長(男女共同参画担当)

学歴

  • - 1982年 奈良女子大学 理学研究科 生物学
  • - 1980年 奈良女子大学 理学部 生物学

委員歴

  • 日本動物学会 近畿支部委員 society

    学協会

  • 2021年01月 日本比較内分泌学会 監事

論文

  • Identification of a new theca/interstitial cell-specific genes and its biological role in growth of mouse ovarian follicles at the gonadotropin-independent stage.

    2019年.8月, Frontiers in Endocrinology, 10

  • Carbohydrate-Appended TQNPEN [N,N,N′,N′-Tetrakis(2-quinolylmethyl)-3-aza-1,5-pentanediamine] Derivatives for Fluorescence Detection of Intracellular Cd2+

    Yuji Mikata; Kana Nozaki; Minori Kaneda; Keiko Yasuda; Masato Aoyama; Satoshi Tamotsu; Arimasa Matsumoto

    © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Invited for the cover of this issue is the group of Yuji Mikata from Nara Women's University, Japan. The cover image shows the application of a carbohydrate-appended polyquinoline ligand as a fluorescent sensor for intracellular Cd2+., 2018年06月29日, European Journal of Inorganic Chemistry, 2018 (24), 2731, doi;scopus;scopus_citedby

  • Replacement of quinolines with isoquinolines affords target metal ion switching from Zn2+ to Cd2+ in the fluorescent sensor TQLN (N,N,N ',N '-tetrakis(2-quinolylmethyl)-2,6-bis(aminomethyl) pyridine)

    Yuji Mikata; Ayaka Takekoshi; Minori Kaneda; Hideo Konno; Keiko Yasuda; Masato Aoyama; Satoshi Tamotsu

    A quinoline-based heptadentate ligand, N, N,N',N'-tetrakis(2-quinolylmethyl)- 2,6-bis(aminomethyl) pyridine (TQLN), exhibits a Zn2+ -specific fluorescence increase at 428 nm, which is assigned to excimer emission (I-Zn/I-o = 38, I-Cd/I-Zn = 24%,phi(Zn) = 0.069). In contrast, the isoquinoline counterpart 1-isoTQLN exhibits a Cd2+ -specific fluorescence increase at 365 nm attributable to monomer emission (I-Cd/I-o = 83, I-Zn/I-Cd = 19%,phi(Cd) = 0.015)., 2017年01月, DALTON TRANSACTIONS, 46 (3), 632 - 637, doi;web_of_science

    研究論文(学術雑誌)

  • The protein phosphatase 6 catalytic subunit (Ppp6c) is indispensable for proper post-implantation embryogenesis

    Honami Ogoh; Nobuhiro Tanuma; Yasuhisa Matsui; Natsuki Hayakawa; Ayaka Inagaki; Mami Sumiyoshi; Yuki Momoi; Ayako Kishimoto; Mai Suzuki; Nozomi Sasaki; Tsukasa Ohuchi; Miyuki Nomura; Yuriko Teruya; Keiko Yasuda; Toshio Watanabe; Hiroshi Shima

    Ppp6c, which encodes the catalytic subunit of phosphoprotein phosphatase 6 (PP6), is conserved among eukaryotes from yeast to humans. In mammalian cells, PP6 targets I kappa B epsilon for degradation, activates DNA-dependent protein kinase to trigger DNA repair, and is reportedly required for normal mitosis. Recently, Ppp6c mutations were identified as candidate drivers of melanoma and skin cancer. Nonetheless, little is known about the physiological role of Ppp6c. To investigate this function in vivo, we established mice lacking the Ppp6c phosphatase domain by crossing heterozygous mutants. No viable homozygous pups were born, indicative of a lethal mutation. Ppp6c homozygous mutant embryos were identified among blastocysts, which exhibited a normal appearance, but embryos degenerated by E7.5 and showed clear developmental defects at E8.5, suggesting that mutant embryos die after implantation. Accordingly, homozygous blastocysts showed significant growth failure of the inner cellmass (ICM) in in vitro blastocyst culture, and primary Ppp6c exon4-deficient MEFs showed greatly reduced proliferation. These results establish for the first time that the Ppp6c phosphatase domain is indispensable for mouse embryogenesis after implantation. (C) 2016 Elsevier Ireland Ltd. All rights reserved., 2016年02月, MECHANISMS OF DEVELOPMENT, 139, 1 - 9, doi;web_of_science

    研究論文(学術雑誌)

  • Dwarf males in the epizoic barnacle Octolasmis unguisiformis and their implications for sexual system evolution

    Kota Sawada; Ryuta Yoshida; Keiko Yasuda; Sachi Yamaguchi; Yoichi Yusa

    Descriptions of the diversity of sexual systems in animal taxa such as the thoracican barnacles are needed to study the evolution of sexual systems. Androdioecious systems (coexistence of hermaphrodites and males) are particularly important due to their possible role as evolutionary intermediates in transitions between hermaphroditism and dioecy. In this study, we used histology to examine the sexual system of the crab-epizoic barnacle Octolasmis unguisiformis to determine if dwarf males were present or not; a previous study reported the existence of conspecific-attached individuals, but did not investigate their sexuality. All conspecific-attached individuals were dwarf males, irrespective of their attachment site. However, crab-attached individuals never acted as dwarf males even if they were small and lived together with large individuals. The result emphasizes the importance of attachment to conspecifics, but not to specific sites on conspecifics, in the evolution of dwarf males. Other individuals were hermaphroditic, indicating androdioecy in this species. However, their functional sexuality (that is, whether they actually do act as males) requires further study. The presence of dwarf males in this species supports theoretical predictions that small group size, short-lived habitats, or spatial limitation favor the evolution of dwarf males., 2015年06月, INVERTEBRATE BIOLOGY, 134 (2), 162 - 167, doi;web_of_science

    研究論文(学術雑誌)

  • Sexual system of a symbiotic pedunculate barnacle Poecilasma kaempferi (Cirripedia: Thoracica)

    Sachi Yamaguchi; Sachi Yoshida; Atsushi Kaneko; Kota Sawada; Keiko Yasuda; Yoichi Yusa

    The pedunculate barnacle Poecilasma kaempferi lives on the body surface of deep-sea decapod crustaceans. We observed that some individuals of this species are attached to conspecifics and investigated their reproductive status to determine whether such individuals are functional dwarf males, as reported for other pedunculates. We also experimentally evaluated the effect of attachment site (whether directly attached to the substratum, attached to conspecifics, or in contact with conspecifics) on reproductive status in an aquarium experiment. Both approaches demonstrated that in this species, conspecific-attached individuals are not dwarf males and are indistinguishable from other individuals in terms of reproductive status. Thus, this species should be considered hermaphroditic rather than androdioecious (i.e. males + hermaphrodites). Such conspecific-attached hermaphrodites may represent an example of pre-adaptation for the evolution of dwarf males. © 2014 Taylor & Francis., 2014年, Marine Biology Research, 10 (6), 635 - 640, doi

    研究論文(学術雑誌)

  • 8-TQEN (N,N,N′,N′-tetrakis(8-quinolylmethyl)ethylenediamine) analogs as fluorescent cadmium sensors: Strategies to enhance Cd 2+-induced fluorescence and Cd2+/Zn2+ selectivity

    安田恵子

    In order to exploit the untapped sensing potential of the TQEN (N,N,N',N'-tetrakis(2-quinolylmethyl)ethylenediamine) family of fluorescent probes, 8-TQEN (N,N,N',N'-tetrakis(8-quinolylmethyl)ethylenediamine) analogs were designed and characterized. Although 8-TQEN lacks practicality owing to poor solubility in both aqueous media and organic solvents, 6-MeO-8-TQEN (N,N,N',N'-tetrakis(6-methoxy-8-quinolylmethyl) ethylenediamine, 1b) exhibits Cd2+-selective fluorescence enhancement at 395 nm in DMF-HEPES buffer (1 : 1), (I-Cd/I-0 = 25, lambda(ex) = 332 nm, phi(Cd) = 0.025). Zn2+ induces weaker fluorescence (I-Zn/I-0 = 9, I-Zn/I-Cd = 0.35). In contrast, both the parent probes TQEN (I-Zn/I-0 = 23, I-Cd/I-Zn = 0.64) and 6-MeOTQEN (I-Zn/I-0 = 11, I-Cd/I-Zn = 1.29 exhibit higher sensitivity toward Zn2+. When the quinoline groups were replaced with 8-hydroxyquinoline different responses were observed. The propanediamine derivative, 8-TQOEPN (N,N,N',N'-tetrakis(8-quinolyloxyethylene)propanediamine, 2c) exhibits significant fluorescence enhancement at 423 nm upon Cd2+ binding (lambda(ex) = 325 nm, I-Cd/I-0 = 19, phi(Cd) = 0.31). Fluorescence enhancement is Cd2+-specific as Zn2+ induces only more modest emission increases (I-Zn/I-0 = 8.8, I-Zn/I-Cd = 0.45)., 2014年, RSC ADVANCES, 4 (25), 12849 - 12856, doi;web_of_science

    研究論文(学術雑誌)

  • Isoquinoline-derivatized tris(2-pyridylmethyl)-amines as fluorescent zinc sensors with strict Zn2+/Cd2+ selectivity

    Yuji Mikata; Keiko Kawata; Saaya Takeuchi; Kaori Nakanishi; Hideo Konno; Saori Itami; Keiko Yasuda; Satoshi Tamotsu; Shawn C. Burdette

    Tris(2-pyridylmethyl)amine-based fluorescent ligands, N,N-bis(1-isoquinolylmethyl)-2-pyridylmethyl-amine (1-isoBQPA) and N,N-bis(7-methoxy-1-isoquinolylmethyl)-2-pyridylmethylamine (7-MeO-1-isoBQPA), have been prepared and the Zn2+-induced fluorescence enhancement has been investigated. Upon excitation at 324 nm, 1-isoBQPA exhibits a very weak emission (phi = similar to 0.010) in DMF-H2O (1 : 1). Upon Zn2+ addition, the 1-isoBQPA fluorescence increases (phi(Zn) = 0.055) at 357 nm and 464 nm. The fluorescence enhancement at longer wavelengths is Zn2+-specific, whereas Cd2+ induces a small emission increase at 464 nm (I-Cd/I-0 = 1.1, I-Cd/I-Zn = 14%). The Zn2+/Cd2+ selectivity of the fluorescent response correlates with the Cd-N-isoquinoline and Zn-N-isoquinoline bond distances measured in the crystal structures. Introduction of methoxy groups into the 1-isoBQPA chromophore enhances the fluorescence significantly (phi(Zn) = 0.213), which affords 7-MeO-1-isoBQPA properties amenable for fluorescence microscopy in living cells., 2014年, DALTON TRANSACTIONS, 43 (28), 10751 - 10759, doi;web_of_science

    研究論文(学術雑誌)

  • Theca Cell Layer Formation in Mouse Ovarian Follicle Culture in vitro

    Saori Itami; Keiko Yasuda; Satoshi Tamotsu; Atsushi Sakai

    2012年09月, CYTOLOGIA, 77 (3), 288 - 288, web_of_science

    研究論文(学術雑誌)

  • Synthesis of Rhenium(I) Tricarbonyl Complexes with Carbohydrate-Pendant Tridentate Ligands and Their Cellular Uptake

    Yuji Mikata; Kyoko Takahashi; Yuka Noguchi; Masami Naemura; Anna Ugai; Saori Itami; Keiko Yasuda; Satoshi Tamotsu; Takashi Matsuo; Tim Storr

    Twelve [ReIL(CO)(3)](n+) complexes with various carbohydrate-pendant ligands L have been prepared and their uptake into HeLa S3 cells were investigated. The ligand library includes: (i) glucose/galactose as the carbohydrate group; (ii) bis(2-pyridylmethyl) amine (DPA), bis(2-quinolylmethyl)amine (DQA), or N-(2-pyridylmethyl)glycine (NPG) as the metal binding component; and (iii) an ethylene chain as a linker between the metal binding site and the O/C-glycosides. Microwave induced plasma mass spectroscopy (MIP-MS) measurements revealed that all complexes were extensively incorporated into the HeLa cells over a 24 h period, and the DQA complexes showed the highest uptake of all the complexes in the series. However, in comparison to the corresponding Re complexes without the pendant carbohydrate functions (prepared with the related ligands LDPA, LDQA, and L-NPG), only the NPG complexes exhibited carbohydrate enhanced cellular uptake. Considering their water solubility and cellular uptake properties, the NPG complexes containing an O-glycoside group (L1 and L'1) are the best candidates for enhancing cellular uptake of metal ions. Microscopic analysis with PC-12 cells in the presence of the fluorescent complex [Re(L'7)(CO)(3)]Cl, revealed that the complex stays in the cell cytosol and cannot penetrate into the nucleus., 2012年01月, EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2012 (2), 217 - 225, doi;web_of_science

    研究論文(学術雑誌)

  • Quinoline-Based, Glucose-Pendant Fluorescent Zinc Probes

    Yuji Mikata; Anna Ugai; Keiko Yasuda; Saori Itami; Satoshi Tamotsu; Hideo Konno; Satoshi Iwatsuki

    Quinoline-based tetradentate ligands with glucose pendants, N,N'-bis[2-(beta-d-glucopyranosyloxy)ethyl]-N,N'-bis[(6-methoxyquinolin-2-yl)methyl]ethylenediamine (N,N'-6-MeOBQBGEN) and its N,N-counterpart, N,N-6-MeOBQBGEN, have been prepared, and their fluorescence-spectral changes upon Zn binding were investigated. Upon excitation at 336 nm, N,N'-6-MeOBQBGEN showed weak fluorescence (?approximate to 0.016) in HEPES buffer (HEPES 50 mM, KCl 100 mM, pH 7.5). In the presence of Zn, N,N'-6-MeOBQBGEN exhibited a significant increase in fluorescence (?=0.096) at 414 nm. The fluorescence enhancement is specific for Zn and Cd (ICd/IZn of 50% at 414 nm). On the other hand, N,N-6-MeOBQBGEN exhibited a smaller fluorescence enhancement upon Zn complexation (?=0.043, ?ex=334 nm, ?em=407 nm) compared with N,N'-6-MeOBQBGEN. Fluorescence microscopic analysis using PC-12 rat adrenal cells revealed that N,N'-6-MeOBQBGEN exhibits a 1.8-fold higher fluorescence-signal response to Zn ion concentration compared with sugar-depleted compound 2 (N,N'-bis[(6-methoxyquinolin-2-yl)methyl]ethylenediamine), due to its enhanced uptake into cells due to the targeting ability of the attached carbohydrates., 2012年09月, CHEMISTRY & BIODIVERSITY, 9 (9), 2064 - 2075, doi;web_of_science

    研究論文(学術雑誌)

  • The maize coleoptiles do not perform typical C4 photosynthesis: investigation \nwith special reference to anatomy, photosynthetic property, and gene \nexpression.

    安田恵子

    2012年, Plant Physiology, 24, 111-121

  • Co-culturing of follicles with interstitial cells in collagen gel reproduce follicular development accompanied with theca cell layer formation

    Saori Itami; Keiko Yasuda; Yuka Yoshida; Chiyuki Matsui; Sachie Hashiura; Atsushi Sakai; Satoshi Tamotsu

    Background: The mechanism of theca cell layer formation in mammalian ovaries has not been elucidated; one reason is that there is no follicle culture system that can reproduce theca cell layer formation in vitro. Therefore, a three-dimensional follicle culture system that can reproduce theca cell layer formation is required. Methods: A collagen gel was used in the follicle culture system. To determine the optimum conditions for follicle culture that can reproduce theca cell layer formation, the effects of hormonal treatment and cell types co-cultured with follicles were examined. In addition, immunohistochemistry was used to examine the properties of the cell layers formed in the outermost part of follicles. Results: Follicles maintained a three-dimensional shape and grew in collagen gel. By adding follicle-stimulating hormone (FSH) and co-culturing with interstitial cells, the follicles grew well, and cell layers were formed in the outermost part of follicles. Immunohistochemistry confirmed that the cells forming the outermost layers of the follicles were theca cells. Conclusion: In this study, follicle culture system that can reproduce theca cell layer formation in vitro was established. In our opinion, this system is suitable for the analysis of theca cell layer formation and contributes to our understanding of the mechanisms of folliculogenesis., 2011年12月, REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 9, 159-167, doi;web_of_science

    研究論文(学術雑誌)

  • The roles of Thy-1 and integrin beta3 in cell adhesion during theca cell layer formation, and the effect of \nfollicle-stimulating hormone on Thy-1 and integrin beta3 localization in mouse ovarian follicles.

    安田恵子

    2011年01月, Biology of Reproduction, 84, 986-995

  • Methoxy-substituted isoTQEN family for enhanced fluorescence response toward zinc ion

    Yuji Mikata; Azusa Yamashita; Keiko Kawata; Hideo Konno; Saori Itami; Keiko Yasuda; Satoshi Tamotsu

    Previously, we have reported that 1- and 3-isoTQENs (N, N, N', N'-tetrakis(1- or 3-isoquinolylmethyl)ethylenediamines) exhibit a specific fluorescence enhancement toward zinc ion. In this study, three methoxy-substituted derivatives of 1-isoTQEN were synthesized and their fluorescent response toward zinc ion was studied. The substitution pattern of the methoxy group significantly changes the solubility of compounds in aqueous DMF, lambda(max) in the absorption spectra, excitation/emission wavelengths and fluorescence intensity of zinc complexes. In the presence of zinc ion, 7-MeO-1-isoTQEN exhibits higher fluorescence intensity and longer excitation/emission wavelengths (lambda(ex) = 342 nm, lambda(em) = 526 nm) than 6-MeO-1-isoTQEN (lambda(ex) = 303 nm, lambda(em) = 469 nm) and 5,6,7-triMeO-1-isoTQEN (lambda(ex) = 340 nm, lambda(em) = 504 nm). The fluorescence intensity of a zinc complex of 7-MeO-1-isoTQEN (phi = 0.122) is four times higher than the parent 1-isoTQEN (phi = 0.034) under the same conditions. The crystal structure of 7-MeO-1-isoTQEN-Zn complex reveals that all six nitrogen atoms participate to the metal coordination with ideal octahedral geometry, affording significantly high metal binding affinity comparable with TPEN (N, N, N', N'-tetrakis(2-pyridylmethyl)ethylenediamine). 7-MeO-1-isoTQEN detects zinc ion concentration change in cells by fluorescence microscopic analysis., 2011年, DALTON TRANSACTIONS, 40 (16), 4059 - 4066, doi;web_of_science

    研究論文(学術雑誌)

  • Methoxyquinoline-diethylenetriamine conjugate as a fluorescent zinc sensor

    Yuji Mikata; Azusa Yamashita; Keiko Kawata; Hideo Konno; Saori Itami; Keiko Yasuda; Satoshi Tamotsu

    A 6-methoxyquinoline conjugated diethylenetriamine derivative, N,N ''-bis(6-methoxy-2-quinolylmethyl) diethylenetriamine (6-MeOBQDIEN) has been synthesized and its fluorescent response toward zinc ion was investigated. In the presence of zinc ion, 6-MeOBQDIEN exhibits fluorescence (lambda(ex) = 329 nm, lambda(em) = 418 nm, phi = 0.039). The fluorescent intensity of the zinc complex of the compound is two times higher than the parent BQDIEN (phi = 0.021) under the same conditions. The crystal structure of 6-MeOBQDIEN-Zn complex shows that all five nitrogen atoms participate to the metal coordination in a distorted square-pyramidal geometry (tau = 0.145) with the aliphatic nitrogen in an apical position. Fluorescent microscopic analysis using 6-MeOBQDIEN reveals the zinc ion concentration change in living cells., 2011年, DALTON TRANSACTIONS, 40 (18), 4976 - 4981, doi;web_of_science

    研究論文(学術雑誌)

  • Development of single shot soft x-ray contact microscopy system for nano-scale dynamics measurement of living biological specimen

    Maki Kishimoto; Masataka Kado; Masahiko Ishino; Satoshi Tamotsu; Keiko Yasuda; Kunio Shinohara

    We have been developing a picosecond single shot soft x-ray contact microscopy system for observing the nanometer-scale inner structure of the living biological specimen in a hydrated condition. The microscopy system consists of an intense IR pump laser system for generating laser-induced plasma as a soft x-ray source and x-ray microscope chamber. The pump laser system employs OPCPA (Optical Parametric Chirped Pulse Amplification) technique to obtain a high contrast pump laser pulse, and we can generate water-window x-rays effectively by combining it to an ultra-thin metal target. The x-ray microscope chamber is composed of a vacuum chamber, a focusing lens, a metal film target, an in-vacuum type sample holder. The pump laser pulse is focused on the metal film target with a focusing lens. The soft x-rays from the laser-induced plasma illuminates bio-specimens on the PMMA photo resist set in the in-vacuum sample holder. The photo resist is developed and the x-ray transmission image recorded on the photo resist is read out by AFM. We took x-ray images of hydrated Leydig cells from mouse testicle and demonstrated that the developed x-ray microscopy system has a spatial resolution of about 100 nm., 2012年, LASER-DRIVEN RELATIVISTIC PLASMAS APPLIED TO SCIENCE, ENERGY, INDUSTRY, AND MEDICINE, 1465, 43 - 47, doi;web_of_science

    研究論文(国際会議プロシーディングス)

  • Observation of actin filaments in Leydig cells with a contact-type soft x-ray microscope with laser plasma x-ray source.

    安田恵子

    2010年, IEEJ Trans, 130 (1774-1778)

  • Effects of chloroplast dysfunction in subpopulation of leaf mesophyll cells on photosynthetic and respiratory activities of a whole leaf: A preliminary study using variegated leaves of Hedera helix L.

    安田恵子

    2009年, plant Morphology, 21 (1), 87-91

  • Changes in the distribution of tenascin and fibronectin in the mouse ovary during folliculogenesis, atresia, corpus luteum formation and luteolysis

    K Yasuda; E Hagiwara; A Takeuchi; C Mukai; C Matsui; A Sakai; S Tamotsu

    Tenascin and fibronectin are components of the extracellular matrices that oppose and promote adhesion, respectively. Using immunohistochemical techniques, we studied the distribution of tenascin and fibronectin in the mouse ovary, in which dynamic reconstruction and degeneration occur during folliculogenesis, atresia, ovulation, corpus luteum formation and luteolysis. In growing follicles, tenascin was only detected in the theca externa layer, while fibronectin was detected in the theca externa layer, theca interna layer and basement membrane. During follicular atresia, granulosa cells, which are surrounded by the basement membrane, began to die through apoptosis. In atretic follicles, tenascin was detected in the basement membrane and theca externa layer. Distribution of fibronectin in atretic follicles was similar to that in healthy growing follicles, except that granulosa cells were slightly immunopositive for fibronectin. In young corpus luteum, luteal cells exhibit high 3 beta-hydroxysteroid dehydrogenase (3-HSD) activity, an enzyme indispensable for progesterone production. Tenascin was barely detected in young luteal cells. 3 beta-HSD activity in luteal cells declines with corpus luteum age, and in older corpus luteum there is an increase in apoptotic death of luteal cells. Tenascin was intensely immunopositive in old luteal cells. In contrast, fibronectin immunostaining in luteal cells was relatively constant during corpus luteum formation and luteolysis. Our observations suggest that tenascin is critical in controlling the degenerative changes of tissues in mouse ovaries. Moreover, in all circumstances observed in this study, tenascin always co-localized with fibronectin, suggesting fibronectin is indispensable for the function of tenascin., 2005年02月, ZOOLOGICAL SCIENCE, 22 (2), 237 - 245, web_of_science

    研究論文(学術雑誌)

  • Vitellogenin-immunohistochemistry in the liver and the testis of Medaka, Oryzias laptis, exposed to 17β-estradiol and p-nonylphenol.

    安田恵子

    メダカ雄個体の17β-estradilolとp-nonyphenol暴露によって、肝臓では肝細胞に、精巣では間質細胞と生殖細胞に\nvitellogeninの存在が確認された。17β-estradilolとp-nonyphenol暴露により肝細胞で産生されたvitellogeninが精巣の生殖細胞によって取り込まれ、蓄積されたことが示唆された, 2005年04月, ZOOLOGICAL SCIENCE, 22 (4), 453 - 461, web_of_science

    研究論文(学術雑誌)

  • Effects of estrogen (17β-estradiol) and p-\n nonylphenol on the development of immune organs in male Japanese quail.

    安田恵子

    ニホンウズラに17β-estradilolとp-nonyphenolを投与し、免疫器官に対する影響について検討した。17β-estradilolと\n p-nonyphenolによってウズラの脾臓、胸腺においてリンパ球の現象や結合組織の増加等の変化が起こり、免疫機能に\n 影響することが示唆された。, 2005年, Environmental Sciences, 12, 99-110

  • Identification of a new theca/interstitial cell-specific gene and its biological role in growth of mouse ovarian follicles at the gonadotropin-independent stage.

    Aoyama M; Shiraishi A; Matubara S; Horie K; Osugi T; Kawada T; Yasuda K; Satake H

    2019年08月, Frontiers in Endocrinology, 10

  • In situ observation of cellular organelles with a contact x-ray microscope

    M. Kado; M. Kishimoto; S. Tamotsu; K. Yasuda; K. Shinohara

    A contact x-ray microscope coupled with a highly intense laser plasma soft x-ray source has been developed and in situ observations of cellular organelles have been conducted. The soft x-rays were generated by irradiating a high power laser pulse onto a thin-foiled gold target and about 1.3x10(15) photons/sr were obtained, which allowed the inner structures of live wet biological cells to be imaged. Single shot flash imaging is a key technique to image cellular organelles of live biological cells avoiding degradation of the spatial resolution of the images resulting from motion blur and radiation damage. The use of a fluorescence microscope to identify cellular organelles in conjunction with the soft x-ray microscope has allowed several cellular organelles to be identified precisely in the soft x-ray images. Combining the fluorescence microscope with the soft x-ray microscope will be very useful and will establish the technique as a powerful tool to analyze living function of biological cells., 2013年, 11TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY (XRM2012), 463, doi;web_of_science

    研究論文(国際会議プロシーディングス)

  • Observation of Organelles in Leydig Cells by Contact Soft X-Ray Microscopy with a Laser Plasma X-Ray Source

    M. Kado; M. Ishino; S. Tamotsu; K. Yasuda; M. Kishimoto; M. Nishikino; Y. Kinjo; K. Shinohara

    We observed the same biological specimens for comparison of the images by contact soft x-ray microscopy with a laser plasma x-ray source with those by confocal laser microscopy. Images of wet Leydig cells were directly comparable for organelles and showed that actin filaments and mitochondria were clearly identified in the soft x-ray images., 2011年, 10TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY, 1365, 391 - 394, doi;web_of_science

    研究論文(国際会議プロシーディングス)

  • The localization and role of Thy-1 during folliculogenesis in mouse ovary

    Saori Itami; Keiko Yasuda; Satoshi Tamotsu

    2006年12月, ZOOLOGICAL SCIENCE, 23 (12), 1217 - 1217, web_of_science

  • Chenges in the distribution of Tenascin and Fibronectin in murine ovary

    Keiko Yasuda; Emi Hagiwara; Akiko Takeuchi; Chinatsu Mukai; Chiyuki Matsui; Atsushi Sakai; Satoshi Tamotsu

    2004年12月, ZOOLOGICAL SCIENCE, 21 (12), 1336 - 1336, web_of_science

  • Effects of 17β-Estradiol and p-Nonylphenol on the Gonads and the Papillary Process Formation in Vivo and in Vitro.

    安田恵子

    2001年, Environmental Science, 8, 211

  • Relationship between the Number of Annuli of Adult Antenna and the length of Embryonic and Larval Period in ┣DBSamia cynthia ricini(/)-┫DB.

    安田恵子

    In the eri-silkworm, Samia cynthia ricini, the adult antennal flagellum is segmented into many annuli. Although the number of annuli is an important parameter in the morphogenesis of adult antenna, it is not clear when and how the number of annuli is determined. In the present study the fifth instar larva of the eri-silkworm was studied histologically to clarify when the antennal imaginal disk began morphogenesis and when the number of annuli of adult antennal flagellum was determined. In addition we studied whether the length of the embryonic and larval period might have any influence on the number of annuli in the eri-silkworm, and the influence of other factors such as body size and sex was also examined. Serial histological study of the imaginal disk during the larval period suggested that the number of annuli was determined by the second day after gut-purging, since the segmentation of the pupal antenna was almost finished by this time and the number of segments of pupal antenna was nearly equal to the number of annuli of adult antenna. The embryonic and larval period was closely related with the number of annuli. When the insects were reared at 25 degrees C, the number of annuli was almost equal to the number of the days in which the insects passed from oviposition to gut-purging. In addition, the number of annuli tended to increase one by one from 27 to 34 as the embryonic and larval period was extended day by day from 28 to 35 days. When the insects were reared at 18 degrees C, the larval period was doubled, whereas the number of annuli remained in the same range (28-34) as that reared at 25 degrees C. The body size did not correlate with the number of annuli. Although the number of annuli was significantly larger in female than in male, this difference seemed to be due to the difference in the length of the embryonic and larval period in both sexes., 1997年06月, ZOOLOGICAL SCIENCE, 14 (3), 435 - 442, web_of_science

    研究論文(学術雑誌)

  • CYTOKINES STIMULATE DIPEPTIDYL PEPTIDASE-IV EXPRESSION ON HUMAN LUTEINIZING GRANULOSA-CELLS

    H FUJIWARA; M FUKUOKA; K YASUDA; M UEDA; K IMAI; Y GOTO; H SUGINAMI; H KANZAKI; M MAEDA; T MORI

    We have previously reported that dipeptidyl peptidase-IV (DPPIV) is a differentiation antigen for human granulosa cells that is initially expressed during corpus luteum formation. To investigate the involvement of cytokines in luteal cell differentiation, we examined the expression and activity of DPPIV in human luteinizing granulosa cells cultured in vitro. Human granulosa cells obtained from patients who had undergone in vitro fertilization were cultured for 7 days in the absence (controls) or presence of hCG (1 U/mL), tumor necrosis factor-alpha (TNF alpha; 10 ng/ml), or interIeukin 1-alpha (IL-1 alpha; 10 ng/mL). Flow cytometry showed that the percentage of cultured granulosa cells treated with TNF alpha and IL-1 alpha that was positive for DPPIV expression was significantly higher than that in controls (43.7 +/- 5.4% and 43.4 +/- 5.6%, respectively, vs. 21.7 +/- 3.5%; P < 0.01), whereas hCG treatment produced no remarkable difference in DPPIV expression (24.0 +/- 5.2%). The DPPIV activity of cells treated with TNF alpha and IL-1 alpha was also significantly higher than that of controls, whereas hCG treatment produced no significant difference from control values. These findings indicate that TNF alpha and IL-1 alpha stimulate DPPIV expression and activity in human luteinizing granulosa cells in vitro and suggest the involvement of cytokines in the differentiation of granulosa cells during corpus luteum formation., 1994年10月, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 79 (4), 1007 - 1011, web_of_science

    研究論文(学術雑誌)

  • A NEW MONOCLONAL-ANTIBODY (POG-1) DETECTS A DIFFERENTIATION ANTIGEN OF PORCINE GRANULOSA AND THECAL CELLS AND INDICATES HETEROGENEITY OF THECAL-STROMAL CELLS

    H FUJIWARA; M UEDA; M FUKUOKA; K YASUDA; K IMAI; K TAKAKURA; H KANZAKI; H SUGINAMI; T MORI; M MAEDA

    To identify the differentiation antigen of ovarian cells, we raised a murine monoclonal antibody (POG-1 antibody) reactive to porcine granulosa and thecal cells in the ovary. Immunofluorescence staining showed that expression of the POG-1 antigen on granulosa and theca interns cells increased gradually in accordance with follicular development. The thecal cells just outside the basal lamina surrounding the follicles did not express the antigen, whereas some stromal cells around the theca externa layer in the large follicles did express it. These expression profiles indicated the heterogeneity of thecal-stromal cells and that the POG-1 antigen was a differentiation-related antigen of granulosa and thecal cells. Luteal cells also expressed the antigen. In organs other than the ovary, some endocrine and exocrine cells, such as Leydig cells and secretory cells of the breast, expressed the antigen. The POG-1 antigen was purified from granulosa cells by immunoaffinity chromatography. Polyacrylamide gel electrophoresis profiles showed that the antigen consisted of two specific proteins; the major one had a molecular mass of 77, and the other had a molecular mass of 81 kilodaltons. Analysis of the purified POG-1 antigen may contribute to understanding the differentiation mechanism of granulosa and thecal cells., 1994年03月, ENDOCRINOLOGY, 134 (3), 1132 - 1138, web_of_science

    研究論文(学術雑誌)

  • HUMAN-LEUKOCYTE ANTIGEN-DR IS A DIFFERENTIATION ANTIGEN FOR HUMAN GRANULOSA-CELLS

    H FUJIWARA; M UEDA; K IMAI; M FUKUOKA; K YASUDA; K TAKAKURA; H SUGINAMI; H KANZAKI; H INOKO; T MORI; M MAEDA

    We raised a murine monoclonal antibody, OG-3, which reacts with human granulosa cells. Immunohistologically, OG-3 antigen was weakly expressed on the granulosa cells of some growing and atretic follicles, but not on those of preovulatory follicles. After ovulation, the antigen expression rapidly increased on granulosa cells during corpus luteum formation. The antigen expression on granulosa/large luteal cells decreased in the mid-luteal phase, but increased again in the late luteal phase. In early pregnancy, OG-3 antigen expression on large luteal cells increased after 7 wk of gestation. The OG-3 antigen distribution in various organs resembled that of human leukocyte antigen (HLA) class II molecules. An HLA-class II-positive human B cell line (AKIBA) and a murine L-cell transfectant expressing HLA-DR antigen were positive for OG-3 antigen, whereas an HLA-class II-negative human T-cell line and L-cell transfectants expressing HLA-DP and DQ antigens were negative. The molecular mass of OG-3 antigen purified from AKIBA cells was 32-35 kDa. The staining profiles in ovaries with anti-HLA-DR or anti-HLA-class II antibodies were similar to that with OG-3. These results indicate that OG-3 antigen is identical to HLA-DR, and that HLA-DR is a differentiation antigen for human granulosa cells., 1993年10月, BIOLOGY OF REPRODUCTION, 49 (4), 705 - 715, web_of_science

    研究論文(学術雑誌)

  • A differentiation-related molecule on the cell surface of human granulosa cells. (共著)

    安田恵子

    We raised a monoclonal antibody (OG-1) specifically reactive to human ovarian granulosa cells. Indirect immunofluorescence staining of freshly isolated granulosa cells from preovulatory follicles showed the OG-1 antigen on the cell surface of granulosa cells. The antigen was purified from granulosa cells by immunoaffinity chromatography. Under reducing or nonreducing conditions, the sodium dodecyl sulfatepolyacrylamide gel electrophoresis profile of the purified antigen showed two specific protein bands, corresponding to mol wt of 125 and 145 kilodaltons. Immunohistologically, the OG-1 antigen on granulosa cells of primary follicles seemed to increase during follicular development. After ovulation, the antigen was detected on granulosa cells during corpus luteum formation. The antigen expression began to decrease on corpus luteum days 10-12 and disappeared on corpus luteum days 13-14. Luteal cells of pregnancy did not express the OG-1 antigen. Granulosa cells in atretic follicles expressed the OG-1 antigen. On the contrary, thecal or small luteal cells did not express it throughout the menstrual cycle. These results indicate that OG-1 antigen is a differentiation-related surface antigen of human granulosa cells., 1993年04月, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 76 (4), 956 - 961, web_of_science

    研究論文(学術雑誌)

  • Effects of interferon on the steroidogenic Functions and proliferation of immature porcine granulosa cells in culture(共著)

    安田恵子

    Recent studies suggest the relevance of several cytokines to the growth and differentiation of granulosa cells. In the present study, we investigated the effects of interferon (IFN) on the steroidogenic functions and proliferation of immature porcine granulosa cells. Human IFN-alpha inhibited FSH-induced progesterone secretion in a concentration-dependent manner. The effect of IFN-alpha was significant at a concentration as low as 10 pg/ml. Maximal inhibitory concentrations (10-50 ng/ml) of IFN-alpha reduced FSH-induced progesterone secretion by 70%. In contrast, estradiol secretion induced by FSH was significantly enhanced by relatively high concentrations (1-50 ng/ml) of IFN-alpha. IFN-alpha (0.1-10 ng/ml) reduced cAMP generation in response to FSH by as much as 80%, although its effect was not concentration-dependent. The proliferation of cultured granulosa cells was inhibited by IFN-alpha in a concentration-dependent manner. Human IFN-gamma did not affect granulosa cell functions. The stimulation of estradiol secretion and the inhibition of cell proliferation induced by IFN-alpha in cultured porcine granulosa cells in this study are in contrast with the effects of IL-1, which, as we reported previously, inhibited both progesterone and estradiol secretion and stimulated cell growth in these cell cultures. Such differences in the mode of action of cytokines may contribute to the regulation of granulosa cell functions under physiological or pathological conditions., 1992年12月, BIOLOGY OF REPRODUCTION, 47 (6), 931 - 936, web_of_science

    研究論文(学術雑誌)

  • Human luteal cells express dipeptidyl peptidase IV on the cell surface. (共著)

    安田恵子

    We previously reported that human theca interna cells and small luteal cells express membrane-bound aminopeptidase N, and suggested that membrane-bound peptidases are involved in folliculogenesis and luteal function by regulating extracellular peptide concentrations. In this study, we examined the expression of dipeptidyl peptidase IV (DPP IV), which is a membrane-bound peptidase and has its catalytic domain at extracellular sites, in human granulosa cells, thecal cells of growing, preovulatory, and atretic follicles, as well as corpora lutea. Indirect immunofluorescence staining of ovarian tissues with specific monoclonal antibodies revealed that DPP IV was present in large and small luteal cells in corpora lutea. DPP IV peptidase activity was also detected histochemically in corpora lutea. In growing, preovulatory, and atretic follicles, there was weak immunoreactivity and DPP IV peptidase activity on luteinized theca interna cells, but not on granulosa cells. The expression of DPP IV on the cell surface of large and small luteal cells was confirmed by indirect immunofluorescence staining of freshly isolated luteal cells. These results indicate that DPP IV is a useful surface differentiation marker of human luteal cells and suggest that peptidases are involved in luteal function., 1992年11月, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 75 (5), 1352 - 1357, web_of_science

    研究論文(学術雑誌)

  • Interactions between interferon γ , tumor necrosis factor α, and interleukin-1 in modulating progesterone and estradiol production by human luteinized granulosa cells in culture. (共著)

    安田恵子

    We have reported that the cytokines, interleukin-1 (IL-1), tumour necrosis factor alpha (TNFalpha), and interferon (IFN)alpha, beta, and gamma modulate the steroidogenic function of human luteinized granulosa cells in culture. In the present study we examined the interactions between these cytokines in modulating progesterone and oestradiol production by these cells. Neither IL-1 nor TNFalpha had significant effects on human chorionic gonadotrophin (HCG)-stimulated progesterone production, whereas IFNgamma (1 - 10 ng/ml) significantly reduced HCG-stimulated progesterone production by 26-37%. Concomitant treatment with IL-1 (I ng/ml) did not further enhance the inhibitory effect of IFNgamma on HCG-stimulated progesterone production. In contrast, the combination of TNFalpha (1 ng/ml) and IFNgamma (10 ng/ml) acted synergistically to markedly inhibit HCG-stimulated progesterone production by 81%. In addition, IL-1 and TNFalpha, neither of which was effective alone, acted synergistically to reduce significantly HCG-stimulated progesterone production by 30%. The combination of TNFalpha and IFNgamma also markedly inhibited follicle stimulating hormone (FSH)-stimulated oestradiol production by 97%, a significantly greater inhibition than that obtained with either cytokine alone. These results suggest that the cytokines may interact to modulate the steroidogenic function of luteal cells in the developing corpus luteum., 1992年11月, HUMAN REPRODUCTION, 7 (10), 1361 - 1364, web_of_science

    研究論文(学術雑誌)

  • Cytokine modulation of progesterone and estradiol secretion in cultures of luteinized human granulosa cells. (共著)

    安田恵子

    To clarify the possible roles of cytokines in the regulation of luteal cell function, we examined the effects of interferon (IFN), interleukin-1 (IL-1), and tumor necrosis factor (TNF) on progesterone and estradiol secretion in cultures of luteinized human granulosa cells. IFN-gamma reduced hCG-stimulated progesterone secretion in a concentration-dependent manner; at its maximal inhibitory concentration (10 ng/mL), IFN-gamma reduced progesterone secretion to 20% of that in the hCG-stimulated controls. Whereas other IFN (alpha and beta) reproduced the inhibitory effect of IFN-gamma, IL-1 and TNF had no effect on hCG-stimulated progesterone secretion at concentrations of 1 and 10 ng/mL. IFN-gamma also markedly reduced FSH-stimulated estradiol secretion. Unlike their effects on hCG-stimulated progesterone secretion, IL-1 and TNF reproduced the inhibitory effect of IFN-gamma on FSH-stimulated estradiol secretion. IFN-gamma significantly reduced both hCG- and FSH-stimulated cAMP generation in granulosa cells. IL-1 and TNF inhibited FSH-stimulated cAMP generation, but they did not inhibit hCG-stimulated cAMP generation. None of these cytokines reduced forskolin-stimulated cAMP generation, thus suggesting that these cytokines affect steps proximal to cAMP generation without affecting cAMP generation itself. IFN-gamma also reduced progesterone secretion in response to (Bu)2cAMP, suggesting that it also affects steps distal to cAMP generation. This study has demonstrated that cytokines modulate the steroidogenesis of luteinized human granulosa cells in vitro; the results suggest that cytokines may play permissive roles in regulating luteal cell function., 1992年07月, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 75 (1), 254 - 258, web_of_science

    研究論文(学術雑誌)

  • Synergistic actions of cytokines and growth factors in enhancing porcine granulosa cell growth. (共著)

    安田恵子

    In our studies of the growth-promoting effect of a cytokine, interleukin-1 (IL-1), on cultured porcine granulosa cells, we found that the potency of IL-1 action correlated with the serum concentration in the culture medium and that IL-1 acted synergistically with insulin to increase the number of cells in the presence of low serum concentrations (0.1-1%). With granulosa cells maintained in a quiescent state under serum-free conditions, we therefore examined the effects of combined treatment with IL-1 and peptide growth factors, including insulin, on [H-3]thymidine incorporation by these cells. IL-1 by itself enhanced [H-3]thymidine incorporation in a concentration-dependent manner. Moreover, IL-1 acted synergistically with insulin, epidermal growth factor (EGF), or fibroblast growth factor (FGF) to enhance [H-3]thymidine incorporation. Combinations of maximally effective concentrations of insulin (1-mu-g/ml), EGF (1 ng/ml), or FGF (50 ng/ml) with the maximally effective concentration of IL-1 (10 ng/ml) increased the levels of [H-3]thymidine incorporation to 10-, 22-, and 20-fold, respectively, over the control values. Whereas IL-2 (0.1-100 ng/ml) did not affect [H-3]thymidine incorporation, tumor necrosis factor alpha (TNF-alpha) stimulated [H-3]thymidine incorporation by itself and reproduced the actions of IL-1 to act synergistically with insulin, EGF, or FGF. When IL-1 and TNF-alpha were added together in relatively low concentrations (1 ng/ml each), the combination had synergistic effects in enhancing [H-3]thymidine incorporation. The present study demonstrates that cytokines and peptide growth factors act synergistically to markedly enhance porcine granulosa cell growth in vitro., 1992年06月, ENDOCRINOLOGIA JAPONICA, 39 (3), 277 - 288, web_of_science

    研究論文(学術雑誌)

  • Differential expression of aminopeptidase N on human ovarian granulosa and theca cells. (共著)

    安田恵子

    The expression of aminopetidase-N and neutral endopeptidase in human ovarian tissue was examined using specific monoclonal antibodies for each of these peptidases and histochemical staining for enzyme activity. Aminopeptidase-N is a membrane-bound metalloprotease catalyzing the removal of N-terminal amino acids from peptides and was detected by immunofluorescence staining on theca interna cells in secondary follicles and on luteinized thecal cells in preovulatory follicles and corpora lutea. However, aminopeptidase-N was not detected on granulosa cells. Peptidase activity was also detected by histochemical staining on theca interna cells and luteinized thecal cells. Luteinized granulosa cells showed peptidase activity, despite the lack of aminopeptidase-N. Neutral endopeptidase was not detected in ovarian granulosa and thecal cells. These observations indicate that aminopeptidase-N can be a useful surface marker for thecal cells., 1992年01月, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 74 (1), 91 - 95, web_of_science

    研究論文(学術雑誌)

  • Luteinizing hormone induces progesterone receptor gene expression in cultured porcine granulosa cells. (共著)

    安田恵子

    We have examined the effect of LH on the regulation of the progesterone receptor (PR) in cultured porcine granulosa cells. In this study we used the RNase protection assay to evaluate the PR mRNA levels with a porcine cDNA clone isolated by the polymerase chain reaction (PCR) method. This clone was regarded as part of the porcine PR cDNA because of its 98.3% and 95.7% homology to the hormone-binding domain of human PR cDNA in amino acid and nucleotide sequences, respectively. Treatment with LH (500 ng/ml) increased porcine PR mRNA to a maximum level of 8.6 +/- 1.1-fold (mean +/- SE) after 3-h exposure. This induction was mimicked by (Bu)2cAMP as well as by FSH and hCG, and the increased PR caused by LH and (Bu)2cAMP occurred in a dose-dependent manner. Basal and LH-induced PR mRNA levels were not affected by progesterone (100 ng/ml), estrogen (100 ng/ml), and RU 486 (10 ng/ml) at 3 h. The mechanism of the increased PR mRNA levels was studied in the presence of actinomycin-D and cycloheximide. While inhibition of RNA synthesis with actinomycin-D blocked LH-induced PR mRNA expression, inhibition of protein synthesis with cycloheximide increased basal and LH-induced PR mRNA levels. These results indicate that the expression of PR mRNA is positively regulated by LH, and this induction does not require ongoing protein synthesis. There may be a cycloheximide-sensitive mechanism that modulates PR mRNA stability. From our results were suspect that progesterone modulates ovarian function through LH-induced PR in granulosa cells., 1991年09月, ENDOCRINOLOGY, 129 (3), 1621 - 1627, web_of_science

    研究論文(学術雑誌)

  • Inhibitory effects of interleukin-1 on follicle stimulating hormone induction of aromatase activity, progesterone secretion and functional luteinizing hormone receptors in cultures of porcine granulosa cells. (共著)

    安田恵子

    1990年12月, BIOLOGY OF REPRODUCTION, 43 (6), 905 - 912, web_of_science

    研究論文(学術雑誌)

  • Interleukin-2 receptor/p55 (Tac) -inducing activity in porcine follicular fluid(共著)

    安田恵子

    1989年08月, ENDOCRINOLOGY, 125 (2), 618 - 623, web_of_science

    研究論文(学術雑誌)

  • Inhibitory effects of interleukin-1 on luteinizing hormone-stimulated adenosine 3´, 5´-monophosphate accumulation by cultured porcine granulosa cells.

    安田恵子

    1989年07月, ENDOCRINOLOGY, 125 (1), 136 - 143, web_of_science

    研究論文(学術雑誌)

  • Interleukin-1 stimulates growth and inhibits progesterone secretion in cultures of porcine granulosa cells. (共著)

    安田恵子

    1989年02月, ENDOCRINOLOGY, 124 (2), 884 - 890, web_of_science

    研究論文(学術雑誌)

  • Modulation of porcine granulosa cell functions by interleukin-1. (共著)

    安田恵子

    Increasing evidence suggests that functions of the immune system and gonads are closely related with each other. In cultures of granulosa and luteal cells, macrophages have been shown to modulate steroidogenic functions. In this paper we present the modulatory effects of interleukin-1, a cytokine produced predominantly by activated macrophages, on gonadotropin-induced differentiation, as well as growth of cultured porcine granulosa cells. © 1989., 1989年, Steroids, 54 (5), 543 - 552, doi;pubmed

    研究論文(学術雑誌)

  • Interleukin-1 inhibits luteinization of porcine granulosa cells in culture. (共著)

    安田恵子

    1988年01月, ENDOCRINOLOGY, 122 (1), 367 - 369, web_of_science

MISC

  • Chemical Shift Images of Organelles in Leydig cells of Mice Testes

    T. Ejima; Y. Neichi; M. Yanagihara; M. Kado; M. Ishino; K. Yasuda; S. Tamotsu

    Soft X-ray transmission images of Leydig cells of mice testes changing incident wavelength were observed with the use of a contact microscope. After normalization of transmission images, absorbance images were obtained and compared with a visible differential interference image. Some organelles were identified by the image comparison, and absorption spectra of the organelles were obtained from the absorbance images. The absorption spectra show that peak structures are different depending on the observed organelles. The structures and the positions of organelles were clearly identified at C-K absorption., IOP PUBLISHING LTD, 2013年, 11TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY (XRM2012), 463, doi;web_of_science

  • Imaging of fine structures of cellular organelles in hydrated biological cells by a soft x-ray microscope combined with a fluorescence microscope

    Masataka Kado; Maki Kishimoto; Satoshi Tamotsu; Keiko Yasuda; Masato Aoyama; Kunio Shinohara

    We have proposed and developed a new hybrid microscopy system using a soft x-ray microscope and a fluorescence microscope imaging the same biological cells at the nearly same time. Combining the powerful advantages such as high spatial resolution of the soft x-ray microscope and the accurate organelle identification of the fluorescence microscope, we can observe fine structures of the cellular organelles in live hydrated biological cells in situ. Staining the cells with several fluorescent dyes such as Mito-tracker, Phalloidin, and DAPI, the soft x-ray images of the cells have been directly compared with the fluorescent images and the cellular organelles such as mitochondria, actin filaments, and chromosomes in the soft x-ray images have been clearly identified. Since the soft x-ray microscope has higher spatial resolution than that of the fluorescence microscope, not only shape of the cellular organelles but also the fine structures of the cellular organelles of the live biological cells have been clearly observed for the first time. (C) (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only., SPIE-INT SOC OPTICAL ENGINEERING, 2013年, X-RAY LASERS AND COHERENT X-RAY SOURCES: DEVELOPMENT AND APPLICATIONS X, 8849, doi;web_of_science

  • 高輝度軟X線源を用いた軟X線顕微鏡による細胞内器官の高解像観察

    加道雅孝; 岸本牧; 保智己; 安田恵子; 青山雅人; 江島丈雄; 刀祢重信; 篠原邦夫; 篠原邦夫

    2016年03月, 大阪大学レーザーエネルギー学研究センター共同利用・共同研究成果報告書, 2015, 56 - 57, j_global;url

  • 高輝度軟X線源を用いた軟X線顕微鏡による細胞内器官の高解像観察

    加道雅孝; 岸本牧; 保智己; 安田恵子; 青山雅人; 江島丈雄; 篠原邦夫; 篠原邦夫

    2015年03月, 大阪大学レーザーエネルギー学研究センター共同利用・共同研究成果報告書, 2014, 70 - 71, j_global;url

書籍等出版物

  • Effects of endocrine disrupting substance on the development of gonads and immune organs in birds and fishes.

    安田恵子 (, 範囲: 分担)

    2002年

  • Ovarian cytokines in regulation of granulosa cell function. (共著)

    安田恵子

    Progress in Endocrinology : The proceedings of the Ninth International Congress of Endocrinology, Nice, 1993年, 577頁

  • Differential function of Luteal cell types in human and porcine luteogenesis. (共著)

    安田恵子

    Local Regulation of Ovarian Function : The Proceedings of the Second Organon Round Table Conference, Lund, 1992年, 11-15巻277頁

  • Granulosa cell immunotropism. (共著)

    安田恵子

    Recent Advances in Ovarian Function : Basic and Clinical Researches., 1992年, 31頁

  • Growth factor modulation of gonadotropin action on porcine granulosa cells. (共著)

    安田恵子

    Advances in Assisted Reproductive Technologies, 1990年, 93頁

  • Regulation of steroidogenic function during luteinization(共著)

    安田恵子

    Development of Preimplantion Embryos and Their Environment, 1989年, 117頁

講演・口頭発表等

  • マウス卵巣における卵胞選別機構の解明に向けてー抗アポトーシス因子cFLIPの発現に着目してー

    水平遥子

    第44回日本比較内分泌学会大会およびシンポジウム, 2019年11月08日, 2019年11月08日

    ポスター発表

  • マウス卵胞の莢膜細胞層形成過程に対するKit-ligandの関与

    近藤景子

    第44回日本比較内分泌学会大会およびシンポジウム, 2019年11月08日, 2019年11月08日

    ポスター発表

  • マウス卵巣において卵胞刺激ホルモン(FSH)の刺激によってマクロファージが産生するインターフェロンα7の卵胞発育に対する作用

    安田恵子

    日本動物学会第90回大阪大会, 2019年09月12日, 2019年09月12日

    口頭発表(一般)

  • 莢膜/間質細胞特異的遺伝子の同定とゴナドトロピン非依存段階の卵巣内卵胞の成長における生物学的役割

    青山雅人

    日本動物学会第90回大阪大会, 2019年09月12日, 2019年09月12日

    口頭発表(一般)

  • マウス卵巣顆粒膜細胞におけるFas/Fas ligand のアポトーシス誘導作用

    中野奏子

    日本動物学会第90回大阪大会, 2019年09月12日, 2019年09月12日

    口頭発表(一般)

  • マウス卵巣のマクロファージはFSH刺激によってインターフェロンα7を産生する。

    山本万遥; 青山雅人; 保智己; 安田恵子

    第43回日本比較内分泌学会, 2018年11月, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台, false

  • マウス卵巣の卵胞形成過程で起こる莢膜細胞層形成機構の解明-卵胞は間質細胞を誘引し、増殖を促進する-

    近藤景子; 伊丹沙織; 村木彩香; 青山雅人; 保智己; 安田恵子

    第43回日本比較内分泌学会大会, 2018年11月, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台, false

  • 腫瘍壊死因子(TNFα)はマウス卵巣の卵胞閉鎖を誘導するのか?

    水平遥子; 青山雅人; 保智己; 安田恵子

    第43回日本比較内分泌学会大会, 2018年11月, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台, false

  • 莢膜/間質細胞特異的遺伝子の同定とゴナドトロピン非依存段階の卵巣内卵胞の成長における生物学的役割

    青山雅人; 白石慧; 松原伸; 大杉知裕; 奥田利美; 安田恵子

    第43回日本比較内分泌学会大会, 2018年11月, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台, false

  • 腫瘍壊死因子α(TNFα)はマウス卵巣の卵胞閉鎖を誘導するのか?

    水平遥子; 青山雅人; 保智己; 安田恵子

    第89回日本動物学会大会, 2018年09月, 日本動物学会, 札幌コンベンションセンター 札幌, false

  • 莢膜/間質細胞特異的遺伝子の同定とゴナドトロピン非依存段階の卵巣内卵胞の成長における生物学的役割

    青山雅人; 白石慧; 松原伸; 堀江郁; 大杉知裕; 奥田利美; 安田恵子; 佐竹炎

    第89回日本動物学会大会, 2018年09月, 日本動物学会, 札幌コンベンションセンター 札幌, false

  • マウス卵巣の卵胞発育過程で起こる莢膜細胞層形成機構の解明-卵胞は間質細胞を誘引し、増殖を促進する-

    近藤景子; 伊丹沙織; 村木彩香; 青山雅人; 保智己; 安田恵子

    第89回日本動物学会大会, 2018年09月, 日本動物学会, 札幌コンベンションセンター 札幌, false

  • 抱卵するコケゴロモガキOstrea circumpictaの性システム

    安岡法子; 安田恵子; 遊佐陽一

    第65回日本生態学会大会, 2018年03月, 日本生態学会, 札幌コンベンションセンター 札幌, false

  • 性周期におけるマウス卵巣内monocyte chemotactic protein-1(MCP-1)の局在と変動

    鍛祥子; 青山雅人; 保智己; 安田恵子

    第42回日本比較内分泌学会, 2017年11月

  • エストロゲンによって促進されるマウス精巣血管新生におけるProkineticin2の関与

    藤原有紗; 青山雅人; 保智己; 安田恵子

    第42回日本比較内分泌学会, 2017年11月

  • マウス卵巣の卵胞発育/卵胞閉鎖におけるトランスフォーミング増殖因子(TGF-β)の機能

    木曾歩美; 深澤早紀; 青山雅人; 保智己; 安田恵子

    第42回日本比較内分泌学会, 2017年11月

  • ケモカインCCL21のマウス卵巣における局在解析と卵胞発育に対する機能

    堀江郁; 青山雅人; 大杉知裕; 佐竹炎; 安田恵子

    第42回日本内分泌学会, 2017年11月, false

  • マウス卵巣の卵胞発育/卵胞閉鎖における腫瘍壊死因子α(TNFα)の機能

    安田恵子; 内田智子; 白金美帆; 蓮室陽子; 青山雅人; 保智己

    日本動物学会第88回大会, 2017年09月, false

  • トランスクリプトーム解析によるマウス卵巣莢膜・間質細胞特異的発現遺伝子の同定

    青山雅人; 白石慧; 堀江郁; 大杉知裕; 奥田利美; 安田恵子; 佐竹炎

    日本動物学会第88回大会, 2017年09月

  • 性周期におけるマウス卵巣内マクロファージおよび末梢血単球のFSH受容体発現の変動

    鍛祥子; 栗野有加; 青山雅人; 保智己; 安田恵子

    第41回二本比較内分泌学会大会及びシンポジウム, 2016年12月, false

  • トランスクリプトーム解析によるマウス卵巣莢膜・間質細胞特異的発現遺伝子の同定

    青山雅人; 白石慧; 堀江郁; 大杉知裕; 安田恵子; 佐竹炎

    第41日本比較内分泌学会大会およびシンポジウム, 2016年12月

  • マウス精巣血管新生におけるプロキネチシン2の役割

    藤原有紗; 今村美瑛; 青山雅人; 保智己; 安田恵子

    第41回日本比較内分泌学会大会及びシンポジウム, 2016年12月, false

  • Identification of mouse ovarian interstitial cell-specific genes by transcriptome analysis.

    安田恵子

    The 22nd International Congress of Zoology and The 87 th meeting of Zoological Society of Japan, 2016年11月, false

  • Changes of localizations and gene expressions of prokineticins and their receptors by gonadotrpin stimulation in mouse ovaries.

    安田恵子

    CompBiol 2015 Hiroshima, 2015年12月, false

  • The localizations of the transforming growth factor beta(TGF-beta) and its receptors in mouse ovaries,.

    安田恵子

    CompBiol 2015 Hiroshima, 2015年12月, false

  • The role of tumor necrosis factor (TNF)α on apoptosis of granulosa cells during follicle atresia in mouse ovaries.

    安田恵子

    CompBiol 2015 Hiroshima, 2015年12月, false

  • Are the adult Leydig cells migrating from mesonephros in the fetal mouse testes?

    安田恵子

    CompBiol 2015 Hiroshima, 2015年12月, false

  • Identification of mouse interstitial cell-specific genes by transcriptome analysis.

    安田恵子

    CompBiol 2015 Hiroshima, 2015年12月, false

  • トランスクリプトーム解析によるマウス卵巣間質細胞特異的遺伝子の\n同定

    青山雅人; 白石慧; 堀江郁; 安田恵子; 佐竹炎

    日本動物学会第86回大会, 2015年09月, false

  • Localization of Tumor Necrosis Factor Receptor 2(TNFR2) in mouse Ovaries.

    安田恵子

    The annual meeting of the Japanese Society for Comparative Endocrinology, 8th International Sympojium on Amphibian and reptilian Endocrinology and Neurobiology., 2014年11月, true

  • Investigating the differentiation time of interstitial cells in the fetal mouse testis.

    安田恵子

    The annual meeting of the Japanese Society for Comparative Endocrinology, 8th International Sympojium on Amphibian and reptilian Endocrinology and Neurobiology., 2014年11月, true

  • Intense laser-plasma soft-X-ray sources and application\nfor biological X-ray microscopy

    安田恵子

    PLASMA2014, 2014年11月, 新潟, true

  • 生きている細胞の内部構造を直接観察できる軟X線顕微鏡の開発

    加道 雅孝; 岸本 牧; 保 智己; 安田 恵子; 青山 雅人; 刀祢 重信; 篠原 邦夫

    日本分子生物学会, 2014年11月, 横浜, 横浜, false

  • HYBRID IMAGING OF LIVING BIOLOGICAL CELLS WITH A SOFT X-RAY MICROSCOPE AND A FLUORESCENT MICROSCOPE

    安田恵子

    12th International Conference on X-ray MIcroscopy, 2014年10月, Melbourne Australia, true

  • Single flash imaging of live hydrated biological cells by a contact soft\nx-ray microscope coupled with an intense laser-plasma soft x-ray\nsource

    安田恵子

    18th International Microscopy Congress, 2014年09月, Prague, Czech Republic, true

  • マウス卵巣におけるプロキネチシン(PK)およびその受容体の局在

    小野伸子; 青山雅人; 大西彩菜; 安田恵子

    日本動物学会第85回大会, 2014年09月, false

  • 試料周辺の水の層の厚さの制御による密着型軟X線顕微鏡\nの高コントラスト化

    加道雅孝; 岸本牧; 刀祢重信; 保智己; 安田恵子; 青山雅人; 篠原邦夫

    日本応用物理学会第75回大会, 2014年09月, 北海道, false

  • N‐K吸収端近傍における生物細胞内構造のコントラスト

    江島丈雄; 廣瀬遼一; 加道雅孝; 石野雅彦; 青山雅人; 安田恵子; 保智己

    応用物理学会秋季学術講演会, 2014年09月, false

  • マウス精巣ライディッヒ細胞の顕微分光測定

    廣瀬僚一; 江島丈雄; 柳原美廣; 加道雅孝; 石野雅彦; 安田恵子; 青山雅人; 保智己

    日本放射光学会年会・放射光科学合同シンポジウム, 2014年01月, false

  • マウスにおいてタキキニンは二次卵胞成長を促進する

    青山雅人; 川田剛士; 伊丹沙織; 保智己; 安田恵子; 佐竹炎

    日本比較内分泌学会38回大会, 2013年10月, false

  • マウス精巣における新生仔期アンドロゲン投与による成体型ライディッヒ細胞増殖促進作用と成体型ライディヒ細胞の起源

    安田恵子; 西尾紗織; 岳崎乃梨子; 保智己

    日本比較内分泌学会第38回大会, 2013年10月, false

  • マウス精巣におけるProkineticinとProkineticin受容体の局在について

    今村美瑛; 保智己; 安田恵子

    日本比較内分泌学会第38回大会, 2013年10月, false

  • マウスにおいてタキキニンは卵胞成長を促進する

    青山雅人; 川田剛士; 伊丹沙織; 保智己; 安田恵子; 佐竹炎

    日本動物学会第84回大会, 2013年09月, false

  • マウス精巣ライディッヒ細胞の分光顕微観察

    根市侑太郎; 江島丈雄; 柳原美廣; 加道雅孝; 石野雅彦; 保智己; 安田恵子

    応用物理学会東北支部学術講演会, 2012年12月, false

  • 新生仔マウス精巣の_x000B_成体型ライディッヒ前駆細胞の増殖に対する_x000B_アンドロゲンの作用時期

    西尾紗織; 保智己; 安田恵子

    日本動物学会第83回大会, 2012年09月, false

  • マウスにおいてタキキニンは卵細胞成長を促進する

    青山 雅人; 川田 剛士; 伊丹 沙織; 保 智己; 安田 恵子; 佐竹 炎

    日本動物学会第83回大会, 2012年09月, 大阪大学, false

  • In situ observation of cellular organelles with a contact x-ray microscope

    安田恵子

    XRM, 2012年08月, true

  • Chemical shift images of Organella in Leydig cells of mice testis.

    安田恵子

    XRM, 2012年08月, true

  • 密着型X線顕微鏡によるマウスライディヒ細胞の吸収スペクトル

    江島丈雄; 根市侑太郎; 柳原美廣; 石野雅彦; 加道雅孝; 安田恵子; 保智己

    日本応用物理学会, 2012年, false

  • タキキニンはマウス二次卵胞の成長を促進する

    青山 雅人; 川田 剛士; 伊丹 沙織; 保 智己; 安田 恵子; 佐竹 炎

    日本比較内分泌学会第37回大会, 2012年, 福井

  • マウス卵巣の卵胞発育過程で起こる莢膜細胞層形成機構の解明V:卵巣内の間質細胞の集合

    伊丹沙織; 保智己; 安田恵子

    (社)日本動物学会第82回大会, 2011年09月, (社)日本動物学会, 北海道旭川市, false

  • マウス卵巣顆粒膜細胞の増殖にTNFαはTNF receptor Iを介して作用するか?

    白金美帆; 保智己; 安田恵子

    (社)日本動物学会第82回大会, 2011年09月, (社)日本動物学会, 北海道旭川市, false

  • レーザープラズマ軟X線顕微鏡による細胞内器官の構造解析II

    加道雅孝; 岸本牧; 石野雅彦; 保智己; 安田恵子; 篠原邦夫

    日本物理学会2011年秋季大会, 2011年09月, (社)日本物理学会, 富山, false

  • Development of single shot soft x-ray contact microscopy sustem for neno-scale dynamics measurement of living biological specimen

    安田恵子

    The 3rd International Symposium " Laser-Driven Relativistic Plasma Applied to Science, Energy, Industry, and Medicine.", 2011年05月, Kyoto Japan, true

  • 細胞内器官の蛍光顕微鏡像と軟X線顕微鏡像との直接比較

    石野雅彦; 加道雅孝; 保智己; 安田恵子; 篠原邦夫; 三方祐司; 岸本牧; 錦野将元; 大場俊幸; 海堀岳史; 河内哲哉

    光量子科学研究シンポジウム, 2010年06月, false

  • マウス卵巣における卵胞選別機構の解明に向けてー抗アポトーシス因子cFLIPの発現に着目してー

    水平遥子; 青山雅人; 保智己; 安田恵子

    第44回日本比較内分泌学会大会及びシンポジウム, 2019年11月09日, 2019年11月08日, 2019年11月08日

  • マウス卵胞の莢膜細胞層形成過程に対するKit-ligand の関与

    近藤景子; 伊丹沙織; 村木彩香; 青山雅人; 保智己; 安田恵子

    第44回日本比較内分泌学会大会及びシンポジウム, 2019年11月09日, 2019年11月08日, 2019年11月08日

  • マウス卵巣において卵胞刺激ホルモン(FSH)の刺激によってマクロファージが産生するインターフェロンα7の卵胞発育に対する作用

    山本万遥; 鍛祥子; 青山雅人; 保智己; 安田恵子

    日本動物学会第90回大阪大会, 2019年09月20日, 2019年09月12日, 2019年09月12日

  • 莢膜/間質細胞特異的遺伝子の同定とゴナドトロピン非依存段階の卵巣内卵胞の成長における生物学的役割

    青山雅人; 白石慧; 松原伸; 堀江郁; 大杉知裕; 奥田利美; 安田恵子; 佐竹炎

    日本動物学会第90回大阪大会, 2019年09月14日, 2019年09月12日, 2019年09月12日

  • マウス卵巣顆粒膜細胞におけるFas/Fas ligandのアポトーシス誘導作用

    中野奏子; 岡田佐和子; 青山雅人; 保智己; 安田恵子

    日本動物学会第90回大阪大会, 2019年09月12日, 2019年09月12日, 2019年09月12日

担当経験のある科目(授業)

  • 生物科学特別研究Ⅰ (奈良女子大学)

  • 生殖生理学セミナーⅠ (奈良女子大学)

  • 化学生物環境学特別研究Ⅱ (奈良女子大学)

  • 生物環境科学展開実習Ⅲ (奈良女子大学)

  • 生殖生理学演習Ⅱ (奈良女子大学)

  • 生殖生理学特論 (奈良女子大学)

  • 化学生物環境学特別研究Ⅰ (奈良女子大学)

  • 卒業研究Ⅰ (奈良女子大学)

  • 「奈良」女子大学入門 (奈良女子大学)

  • 生殖生理学演習Ⅰ (奈良女子大学)

  • 個体構造学セミナーⅠ (奈良女子大学)

  • 個体機能生物学概論A (奈良女子大学)

  • 生物科学特論8 (奈良女子大学)

  • 発生生物学 (奈良女子大学)

  • 学生生活入門 (奈良女子大学)

  • 課題研究Ⅲ (奈良女子大学)

  • 生物科学特別研究Ⅳ (奈良女子大学)

  • 生物科学特別研究Ⅱ (奈良女子大学)

  • 個体構造学セミナーⅣ (奈良女子大学)

  • 個体構造学セミナーⅡ (奈良女子大学)

  • 生物環境科学演習 (奈良女子大学)

  • 展開実習Ⅲ (奈良女子大学)

  • 課題研究Ⅱ (奈良女子大学)

  • 卒業研究Ⅲ (奈良女子大学)

  • 卒業研究Ⅱ (奈良女子大学)

  • 個体構造学特論Ⅱ (奈良女子大学)

  • 個体構造学セミナーⅢ (奈良女子大学)

  • 個体構造学セミナーI (奈良女子大学)

  • 生物科学特別研究Ⅲ (奈良女子大学)

  • 生物科学特別研究I (奈良女子大学)

  • 生物環境科学基礎演習Ⅰ (奈良女子大学)

  • 生物環境科学基礎実習Ⅰ (奈良女子大学)

  • 個体・集団生物学特論1 (奈良女子大学)

  • 課題研究Ⅳ (奈良女子大学)

  • 課題研究Ⅰ (奈良女子大学)

  • 卒業研究Ⅳ (奈良女子大学)

  • 海洋生物学野外実習 (奈良女子大学)

  • 個体・集団生物学特論3 (奈良女子大学)

  • 課題研究III (奈良女子大学)

  • 課題研究II (奈良女子大学)

  • 森林生物学野外実習 (奈良女子大学)

  • 基礎生物学実習Ⅰ (奈良女子大学)

  • 卒業研究IV (奈良女子大学)

  • 課題研究IV (奈良女子大学)

  • 課題研究I (奈良女子大学)

  • 生物環境科学基礎演習IB (奈良女子大学)

  • 生物環境科学基礎演習IA (奈良女子大学)

  • 生物環境科学基礎実習IB (奈良女子大学)

  • 生物環境科学基礎実習IA (奈良女子大学)

  • 生物科学方法論 (奈良女子大学)

  • 生殖生理演習 (奈良女子大学)

  • 生殖生理論 (奈良女子大学)

  • 卒業研究IV (奈良女子大学)

  • 展開実習III (奈良女子大学)

  • 卒業研究III (奈良女子大学)

  • 基礎生物科学実習I (奈良女子大学)

  • 生物科学特論8 (奈良女子大学)

  • 生物科学特論1 (奈良女子大学)

  • 生物学実験I (奈良女子大学)

  • 基礎生物科学演習 (奈良女子大学)

  • 生物学実験I (奈良女子大学)

  • 個体構造学セミナーIV (奈良女子大学)

  • 個体構造学セミナーII (奈良女子大学)

  • 卒業研究II (奈良女子大学)

  • 生物学特別講義IIC (奈良女子大学)

  • 個体構造学特論II (奈良女子大学)

  • 卒業研究I (奈良女子大学)

  • 生物学特別講義V (奈良女子大学)

  • 生物学演習II (奈良女子大学)

  • 基礎生物学実習I (奈良女子大学)

  • サイエンスオープンラボI (奈良女子大学)

  • 展開実習 (奈良女子大学)

  • 陸圏生物学野外実習 (奈良女子大学)

  • 個体構造学特論IV (奈良女子大学)

  • 卒業研究II (奈良女子大学)

  • 動物形態分類学実習 (奈良女子大学)

  • 卒業研究I (奈良女子大学)

  • 生物形態発生学実習 (奈良女子大学)

  • 恒常性の生理学 (奈良女子大学)

  • 個体構造学セミナーIV (奈良女子大学)

  • 個体構造学セミナーII (奈良女子大学)

  • 動物分類学実習 (奈良女子大学)

  • 形態学実習 (奈良女子大学)

  • 動物形態学 (奈良女子大学)

  • 個体構造学特論II (奈良女子大学)

  • 特別講義XIII (奈良女子大学)

  • 基礎生物学実習I (奈良女子大学)

  • 発生生物学実習 (奈良女子大学)

  • 形態形成学 (奈良女子大学)

  • 生物学序説 (奈良女子大学)

  • ジェンダー論入門 (奈良女子大学)

  • 総合演習G (奈良女子大学)

所属学協会

  • 日本動物学会

  • 日本発生生物学会

  • 日本比較内分泌学会

  • 日本内分泌学会

  • 日本生殖内分泌学会

Works(作品等)

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    2017年04月 - 2018年03月

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    2016年04月 - 2017年03月

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    2015年04月 - 2016年03月

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    2014年04月 - 2015年03月

  • 高輝度軟X 線源を用いた軟X 線顕微鏡による細胞内器官の高解像観察

    2014年04月 - 2015年03月

  • 軟X線による生きている細胞の生理現象の観察

    2014年04月 - 2015年03月

  • マウス精巣ライディッヒ細胞の顕微分光測定

    2014年04月 - 2015年03月

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    2014年04月 - 2015年03月

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    2013年04月 - 2014年03月

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    2012年04月 - 2013年03月



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