研究者総覧

前田 純夫 (マエダ スミオ)

  • 研究院生活環境科学系食物栄養学領域 准教授
メールアドレス:
smaedacc.nara-wu.ac.jp
Last Updated :2021/06/02

researchmap

学位

  • 農学博士, 名古屋大学

研究キーワード

  • <国際的にも独創性の高い研究を目指しています。> <研究室web → http://food.moon.bindcloud.jp/staffdetail/pg1548851.html> <大学院生を広く募集します。「実力を高めること」が当研究室教育の第一目標です。> 抗生物質耐性菌・耐性遺伝子 パーシスター細胞 大腸菌 バイオフィルム 遺伝子水平伝播 微生物 persister antibiotic-resistant bacteria Escherichia coli Biofilm Horizontal gene transfer Microbiology 

研究分野

  • ライフサイエンス, 遺伝学
  • ライフサイエンス, 細菌学
  • ライフサイエンス, 応用微生物学
  • ライフサイエンス, 応用分子細胞生物学

受賞

  • 日本細菌学会総会優秀発表賞, 望月美奈子(研究グループ責任者:前田純夫), 日本細菌学会, 2020年02月, japan_society

論文

  • High temperatures promote cell-to-cell plasmid transformation in Escherichia coli

    Hashimoto M; Hasegawa H; Maeda S

    2019年06月, Biochemical and Biophysical Research Communications, 515, 196

  • Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms

    Hasegawa H; Suzuki E; Maeda S

    2018年10月, Frontiers in Microbiology, 9, 2365, rm:research_project_id

  • Bacterial memory of persisters: Bacterial persister cells can retain their phenotype for days or weeks after withdrawal from colony–biofilm culture

    Saki Miyaue; Erika Suzuki; Yoko Komiyama; Yu Kondo; Miki Morikawa; Sumio Maeda

    2018年, Frontiers in Microbiology, 9, 1396, doi;rm:research_project_id

  • Bacteriophage P1vir-induced cell-to-cell plasmid transformation in Escherichia coli

    Chiaki Sugiura; Saki Miyaue; Yuka Shibata; Akiko Matsumoto; Sumio Maeda

    2017年, AIMS Microbiology, 3 (4), 784 - 797, doi;rm:research_project_id

  • Competence development and horizontal plasmid transfer in natural Escherichia coli strains

    Akiko Matsumoto; Ayuka Sekoguchi; Yukako Murakami; Junko Imai; Kumiko Kondo; Yuka Shibata; Sumio Maeda

    2016年, Microbes in the spotlight: recent progress in the understanding of beneficial and harmful microorganisms, 468 - 473, rm:research_project_id

  • Natural Escherichia coli strains undergo cell-to-cell plasmid transformation

    Akiko Matsumoto; Ayuka Sekoguchi; Junko Imai; Kumiko Kondo; Yuka Shibata; Sumio Maeda

    2016年, Biochemical and Biophysical Research Communications, 481, 59 - 62, doi;rm:research_project_id

  • 大腸菌標準野生株(ECOR strains)におけるバイオフィルム形成能の分析

    柴田有加; 松本晃子; 世古口歩華; 前田純夫

    2014年, 家政学研究, 60 (2), 111 - 116

  • Genome-wide screen for Escherichia coli genes involved in repressing cell-to-cell transfer of non-conjugative plasmids

    Matsuda, A; Kurono, N; Kawano, C; Shirota, K; Hirabayashi, A; Horino, M; Etchuya, R; Sobue, R; Sasaki, Y; Miyaue, S; Sekoguchi, A; Sugiura, C; Shibata, Y; Ito, M; Ando, T; Maeda, S

    2012年, Biochemical and Biophysical Research Communications, 428, 445 - 450, doi;rm:research_project_id

  • Genome-wide screening of Escherichia coli genes involved in execution and promotion of cell-to-cell transfer of non-conjugative plasmids: rodZ (yfgA) is essential for plasmid acceptance in recipient cells.

    Naomi Kurono; Ayako Matsuda; Rika Etchuya; Rina Sobue; Yumi Sasaki; Miki Ito; Tsuyako Ando; Sumio Maeda

    2012年, Biochemical and Biophysical Research Communications, 421, 119 - 123, doi;rm:research_project_id

  • Genome-wide screening of Escherichia coli genes involved in repression of peptide-pheromone-regulated natural transformation

    MAEDA Sumio; Ayako Matsuda; Naomi Kurono; Chinatsu Kawano; Kozue Shirota; Akiko Hirabayashi; Mutsumi Horino; Rika Etchuuya; Rina Sobue; Yumi Sasaki; Tsuyako Ando; Miki Ito; Sumio Maeda

    2011年, Peptide Science 2010, 81

  • Genome-wide screening of Escherichia coli genes involved in exection and promotion of peptide-pheromone-regulated natural transformation

    Naomi Kurono; Ayako Matsuda; Rika Etchuuya; Rina Sobue; Yumi Sasaki; Tsuyako Ando; Miki Ito; Sumio Maeda

    2011年, Peptide Science 2010, 79

  • Identification of a novel DNA element that promotes cell-to-cell transformation in Escherichia coli.

    Rina Sobue; Naomi Kurono; Rika Etchuya; Sumio Maeda

    2011年, FEBS Letters, 585, 2223 - 2228, doi;rm:research_project_id

  • Cell-to-Cell Transformation in Escherichia coli: A Novel Type of Natural Transformation Involving Cell-Derived DNA and a Putative Promoting Pheromone.

    MAEDA Sumio; Rika Etchuuya; Miki Ito; Seiko Kitano; Fukiko Shigi; Rina Sobue; Sumio Maed

    2011年, PLoS One, 6 (1), e16355, doi;rm:research_project_id

  • Horizontal transfer of non-conjugative plasmid in colony biofilm of Escherichia coli on food-based media

    Ando, T; Maeda, S

    2009年, World Journal of Microbiology and Biotechnology, 25, 1865 - 1869, doi;rm:research_project_id

  • Freeze-thaw-induced lateral transfer of non-conjugative plasmids by in situ transformation in Escherichia coli in natural waters and food extracts

    Ishimoto, Y; Kato, S; Maeda, S

    2008年, World Journal of Microbiology and Biotechnology, 24 (11), 2731-2735, doi:10.1007/s11274-008-9761-z, doi;rm:research_project_id

  • Phi29 DNA polymeraseで増幅した米ゲノムDNAのPCRによる遺伝子多型分析への適用例と条件検討

    伊藤他; 前田 純夫

    2007年, DNA多型, 15, 299 - 303

  • バイオフィルム状微生物の遺伝子転移に関する研究

    前田 純夫

    2007年, IFO Research Communications, 21, 71 - 81

  • Phi29 DNA polymeraseで増幅したヒト口腔粘膜細胞DNAのPCRによる遺伝子多型分析への適用例と条件検討

    伊藤未来; 井上幸子; 前田純夫

    2006年, DNA多型, 14, 46 - 51

  • 微生物バイオフィルムと食品製造環境

    前田純夫

    2006年, 食品機械装置, 43, 48 - 53

  • 食の安心を築くために

    前田純夫

    2006年, 日刊工業新聞, (19871), 14

  • Horizontal Transfer of Nonconjugative Plasmids in a Colony Biofilm of Escherichia coli.

    Maeda, S; Ito, M; Ando, T; Ishimoto, Y; Fujisawa, Y; Takahashi, H; Sawamura, A; Matsuda, A; Kato, S

    2006年, FEMS Microbiology Letters, 255, 115 - 120, doi

  • 遺伝子組換え食品の社会的受容過程の分析

    堺谷展子; 前田純夫

    2005年, 家政学研究, 52, 27 - 38

  • Cell-direct PCR法によるヒトゲノムSNPの簡便検出法

    石本裕子; 前田純夫

    2004年, 家政学研究, 51, 1 - 5

  • 奈良女子大学学生の遺伝子組換え食品への意識調査

    堺谷展子; 前田純夫

    2004年, 家政学研究, 50, 154 - 162

  • Transformation of Colonial Escherichia coli on Solid Media

    Maeda, S; Sawamura, A; Matsuda, A

    2004年, FEMS Microbiology Letters, 236, 61 - 64, doi

  • Purification and Characterization of an Extracellular Laccase of a Fungus (Family Chaetomiaceae) Isolated from Soil.

    Saito, T; Hong, P; Kato, K; Okazaki, M; Inagaki, H; Tanaka, K; Takada, M; Maeda, S; Yokogawa, Y

    2003年, Enzyme and Microbial Technology, 33, 520 - 526, doi

  • バイオテクノロジーと食物

    前田 純夫

    2003年, 食物科学概論, 6.3

  • Competency Development of Escherichia coli in Foodstuffs.

    MAEDA Sumio

    2003年, Microbes & Environment, 18 (2), 100 - 103, doi

  • Spatial Distribution of mDLG6 mRNA in Embryonic and Adult Mouse Brain.

    Inagaki, H; Tanaka, K; Takada, M; Maeda, S; Ichihara, S; Saito, T

    2002年, Cell Biology International, 26, 635 - 640, doi

  • Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA

    Inagaki, H; Hayashi, T; Matsushima, Y; Lin. K.H; Maeda, S; Ichihara, S; Kitagawa, Y; Saito, T

    2002年, DNA Sequence, 11, 131 - 135, doi

  • プロテイントランスダクション:外来タンパク質を細胞内に導入する新方法

    前田 純夫

    2000年, 化学と工業, 53 (4), 519

  • Staurosporine Promotion of Formation of Continuous Monolayers of Primary Rat Hepatocytes by Improving Attachment and Spreading.

    MAEDA Sumio; Maeda, S; Lin, K.H; Inagaki, H; Saito, T

    2000年, Bioscience, Biotechnology, and Biochemistry, 64 (9), 1985 - 1987, doi

  • Mechanisms of Active Cell Death in Isolated Hepatocytes

    MAEDA Sumio

    2000年, The Hepatocyte Review, Chapter 18, 251 - 267

  • rDLG6: a Novel Homolog of Drosophila DLG Expressed in Rat Brain.

    Inagaki, H; Maeda, S; Lin, K.H; Shimizu, N; Saito, T

    1999年, Biochemical and Biophysical Research Communications, 265, 462 - 468, doi

  • Inhibition of Mitochondrial Gene Expression by Antisense RNA of Mitochondrial Transcription Factor A (mtTFA).

    Inagaki, H; Kitano, S; Lin, K.H; Maeda, S; Saito, T

    1998年, Biochemistry and Molecular Biology International, 45, 567 - 573

  • Influence of Aldehyde Groups on the Thermostability of an Immobilized Enzyme on an Inorganic Support.

    Saito, T; Yoshida, Y; Kawashima, K; Lin, K.H; Maeda, S; Kobayashi, T

    1997年, Materials Sci. Eng. C, 5, 149 - 152

  • Long-Term Culture of Rat Hepatocytes on Heparin- or Lambda Carrageenan- containing Collagen Gels

    Lin, K.H; Maeda, S; Inagaki, H; Saito, T

    1997年, Bioscience, Biotechnology, and Biochemistry, 61, 971 - 974, doi

  • アポトーシス(細胞の自殺)誘導機構の解明とその生物工学的利用

    前田 純夫

    1997年, 工業技術, 38 (2), 33

  • アポトーシス(細胞の自殺)誘導機構の解明とその生物工学的利用

    前田 純夫

    1997年, KITEC INFORMATION, 36 (4), 6

  • Promotive Effect of DMSO on TGF-b1-Induced Apoptosis in Primary Culture of Rat Hepatocytes.

    MAEDA Sumio; Maeda, S; Miyazawa, A; Lin, K.H; Inagaki, H; Saito, T

    1997年, Journal of Biochemistry, Molecular Biology and Biophysics, 1, 117 - 124

  • Osteonectin Gene Expression in Fibrotic Liver.

    Inagaki, H; Lin, K.H; Maeda, S; Saito, T

    1996年, Life Sciences, 58, 927 - 934, doi

  • Induction of Apoptosis in Primary Culture of Rat Hepatocytes by Protease Inhibitors.

    Maeda, S; Lin, K.H; Inagaki, H; Saito, T

    1996年, Biochemistry and Molecular Biology International, 39, 447 - 453

  • Albumin Synthesis by Rat Hepatocytes Cultured on Collagen Gels Is Sustained Specifically by Heparin.

    Lin, K.H; Hino, H; Maeda, S; Inagaki, H; Valiakhmetov, A.J; Saito, T

    1995年, Experimental Cell Research, 218, 717 - 721, doi

  • 肝実質細胞と血管内皮細胞の相互作用による増殖と分化機能の発現制御に関する研究

    前田純夫; 林孔華; 稲垣英利; 斎藤隆雄

    1995年, 名古屋工業技術研究所報告, 44, 530 - 546

  • Long-Term Maintenance of Liver-Specific Functions in Three-Dimensional Culture of Adult Rat Hepatocytes with a Porous Gelatin Sponge Support..

    Lin, K.H; Maeda, S; Saito, T

    1995年, Biotechnology and Applied Biochemistry, 21, 19 - 27

  • DNA Fragmentation Induced in High-Cell-Density Culture of Primary Rat Hepatocytes Is an Active Process Dependent on Energy Availability, Gene Expression, and Calmodulin.

    MAEDA Sumio; Maeda, S; Suzuki, A; Lin, K.H; Inagaki, H; Saito, T

    1995年, Journal of Biochemistry, 118, 1161 - 1165

  • Inhibition by Retinoic Acid of Albumin and DNA Synthesis in Adult Rat Hepatocytes.

    Lin, K.H; Maeda, S; Koga, N; Saito, T

    1994年, Bioscience, Biotechnology, and Biochemistry, 58, 584 - 585, doi

  • Immobilization and Characterization of a Thermostable ß-Galactosidase from a Thermophilic Anaerobe on a Porous Ceramic Support

    Saito, T; Yoshida, Y; Kawashima, K; Lin, K.H; Maeda, S; Kobayashi, T

    1994年, Applied and Microbiological Biotechnology, 40, 618 - 621, doi

  • プログラムされた細胞死:アポトーシス(工業技術)

    前田 純夫

    1993年, 工業技術, 34 (6), 23 - 24

  • プログラムされた細胞死 : アポトーシス (通産ジャーナル)

    前田 純夫

    1993年, 通産ジャーナル

  • Cell Density-Dependent DNA Fragmentation and Its Suppression by Heparin in Primary Culture of Adult Rat Hepatocytes.

    MAEDA Sumio; Maeda, S; Kimura, H; Koga, N; Lin, K.H; Saito, T

    1993年, Biochemical and Biophysical Research Communications, 195, 270 - 275, doi

  • A MODIFIED COLORIMETRIC MTT ASSAY ADAPTED FOR PRIMARY CULTURED-HEPATOCYTES - APPLICATION TO PROLIFERATION AND CYTOTOXICITY ASSAYS

    M OKA; S MAEDA; N KOGA; K KATO; T SAITO

    1992年09月, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 56 (9), 1472 - 1473, doi;web_of_science

  • Overproduction of Thermostable ß-Galactosidase in Escherichia coli, Its Purification and Molecular Structure.

    Saito, T; Kato, K; Maeda, S; Suzuki, T; Shiba, S; Iijima, S; Kobayashi, T

    The lacN gene encoding thermostable, beta-galactosidase from a thermophilic anaerobe strain NA10 was overexpressed in Escherichia coli strain MV1184 by cloning the gene downstream from the lac promoter on pUC119. The amount of the enzyme produced by the E. coli transformant was estimated to be about 20% of the total cellular proteins when induced with isopropyl-beta-D-thiogalactopyranoside. Fed-batch culture of this strain resulted in about a 2,400-fold increase in enzyme production as compared to that in the original bacterium, strain NA10. The enzyme was purified, and its molecular structure was determined by SDS-polyacrylamide gel electrophoresis and gel filtration to be a tetrametric protein consisting of identical subunits., 1992年, Journal of Fermentation and Bioengineering, 74 (1), 12 - 16, doi;web_of_science

    研究論文(学術雑誌)

  • Expression of micF Involved in Porin Synthesis in Escherichia coli: Two Distinct Cis-Acting Elements Respectively Regulate micF Expression Positively and Negatively

    Takayanagi, K; Maeda, S; Mizuno, T

    1991年, FEMS Microbiology Letters, 83, 39 - 44, doi

  • Activation of the Osmoregulated ompC Gene by the OmpR Protein in Escherichia coli : A Study Involving Synthetic OmpR-Binding Sequences

    MAEDA Sumio; Maeda, S; Takayanagi, K; Nishimura, Y; Maruyama, T; Mizuno, T

    1991年, Journal of Biochemistry, 3, 324 - 327

  • EVIDENCE FOR MULTIPLE OMPR-BINDING SITES IN THE UPSTREAM ACTIVATION SEQUENCE OF THE OMPC PROMOTER IN ESCHERICHIA-COLI - A SINGLE OMPR-BINDING SITE IS CAPABLE OF ACTIVATING THE PROMOTER

    S MAEDA; T MIZUNO

    1990年01月, JOURNAL OF BACTERIOLOGY, 172 (1), 501 - 503, web_of_science

    研究論文(学術雑誌)

  • Activation of the ompC Gene by the OmpR Protein in Escherichia coli : the Cis-Acting Upstream Sequence Can Function in Both Orientations with Respect to the Canonical Promoter.

    MAEDA Sumio; Maeda; S. Mizuno, T

    1988年, Journal of Biological Chemistry, 263, 14629 - 14633

  • Stereospecific Positioning of the Canonical Promoter Is Required for Activation of the ompC Gene by a Positive Regulator, OmpR, in Escherichia coli.

    MAEDA Sumio; Maeda, S; Ozawa, Y; Mizuno, T; Mizushima, S

    1988年, Journal of Molecular Biology, 202, 433 - 441, doi

  • First and broad detection of three typical carbapenemase genes on the surfaces of commercially available spices worldwide and isolation of complete NDM-1 genes from black pepper, cumin, and clove

    Minako Mochizuki; Sumio Maeda

    ABSTRACTThe spread of multidrug-resistant bacteria, particularly those producing carbapenemases, has become a major public health concern. The presence of carbapenemase genes has primarily been reported in clinical samples, whereas the presence of these genes in commercially available foods has insufficiently been studied despite its growing importance. The present study aimed to detect and characterize carbapenemase genes (blaNDM,blaIMP, blaKPC, and blaOXA-48-like) on the surfaces of commercially available spices using PCR to amplify conserved regions of these genes. It was revealed that DNAs of these genes are commonly present (13 genes/29 samples) on spices derived from at least 9 different countries. This is the first detection of any carbapenemase gene on eight spices (black pepper, cumin, clove, cardamom, mustard, caraway, parsley, and rosemary) among these. This is also the first detection of the blaIMP and blaNDM as well as the broad detection of the blaOXA-48-like on spices. We also isolated complete, functional blaNDM-1 genes from three spices (black pepper, cumin, and clove) up to the present., 2020年06月10日, bioRxiv, 2020, bioRxiv, doi;url

    研究論文(学術雑誌)

  • Natural Transformation in Escherichia coli

    Yoko Komiyama; Sumio Maeda

    2020年, Methods in Molecular Biology "Horizontal Gene Transfer", 2075, 179 - 187, doi;url;rm:research_project_id

    論文集(書籍)内論文

MISC

  • First and broad detection of three typical carbapenemase genes on the surfaces of commercially available spices worldwide and isolation of complete NDM-1 genes from black pepper, cumin, and clove

    Cold Spring Harbor Laboratory, 2020年, bioRxiv, 2020, 14614

書籍等出版物

  • Methods in Molecular Biology "Horizontal Gene Transfer" 2075 179-187, Chapter 13: Natural Transformation in Escherichia coli

    Komiyama Y; Maeda S

    Springer Nature, 2020年

  • 食物科学概論 改訂版

    (, 範囲: 6.3 バイオテクノロジーと食物)

    朝倉書店, 2014年, vii, 165p, cinii_books (ISBN: 9784254606263)

  • 食物科学概論、6.3 バイオテクノロジーと食物

    朝倉書店, 2003年

  • The Hepatocyte Review, Chapter 18: Mechanisms of Active Cell Death in Isolated Hepatocytes

    Maeda S

    Kluwer Academic Publishers, 2000年

講演・口頭発表等

所属学協会

  • 日本微生物生態学会

  • 日本生化学会

  • 日本分子生物学会

  • 日本農芸化学会



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