研究者総覧

杉浦 真由美 (スギウラ マユミ)

  • 研究院自然科学系生物科学領域 准教授
Last Updated :2021/06/02

researchmap

学位

  • 博士(理学), 奈良女子大学

研究キーワード

  • 環境応答 交配フェロモン 有性生殖 繊毛虫 原生生物 

研究分野

  • ライフサイエンス, 細胞生物学
  • ライフサイエンス, 分子生物学

委員歴

  • 2018年10月 日本原生生物学会 評議員 society

    学協会

  • 2017年09月 日本動物学会 男女共同参画委員会委員 日本動物学会 society

    学協会

  • 2015年11月 - 2018年10月 日本原生生物学会 学会活性化委員 日本原生生物学会 society

    学協会

受賞

  • 2018 Joint Meeting of the Korean Society of Protistologists and Japan Society of Protozoology, Best Poster Award, 2018年
  • 日本動物学会女性研究者奨励OM賞, 2005年, JPN
  • 日本原生動物学会奨励賞, 2004年, JPN
  • 第21回井上研究奨励賞, 2004年, JPN

論文

  • A single amino acid residue regulates the substrate affinity and specificity of indoleamine 2,3-dioxygenase

    Hajime J. Yuasa; Mayumi Sugiura; Terue Harumoto

    Indoleamine 2,3-dioxygenase (IDO) is a heme-containing enzyme that catalyses the oxidative cleavage of L-Trp. The ciliate Blepharisma stoltei has four IDO genes (IDO-I, -II, -III and -IV), which seem to have evolved via two sequential gene duplication events. Each IDO enzyme has a distinct enzymatic property, where IDO-III has a high affinity for L-Trp, whereas the affinity of the other three isoforms for L-Trp is low. IDO-I also exhibits a significant catalytic activity with another indole compound: 5-hydroxy-L-tryptophan (5-HTP). IDO-I is considered to be an enzyme that is involved in the biosynthesis of the 5-HTP-derived mating pheromone, gamone 2. By analysing a series of chimeric enzymes based on extant and predicted ancestral enzymes, we identified Asn131 in IDO-I and Glu132 in IDO-III as the key residues responsible for their high affinity for each specific substrate. These two residues were aligned in an identical position as the substrate-determining residue (SDR). Thus, the substrate affinity and specificity are regulated mostly by a single amino acid residue in the Blepharisma IDO-I and IDO-III enzymes., 2018年02月15日, Archives of Biochemistry and Biophysics, 640, 1 - 9, doi

    研究論文(学術雑誌)

  • Novel Specificity of IDO Enzyme Involved in the Biosynthesis of Mating Pheromone in the Ciliate Blepharisma stoltei

    Mayumi Sugiura; Hajime J. Yuasa; Terue Harumoto

    Mating pheromones (gamone 1 and gamone 2) in the ciliate Blepharisma are biologically active substances that trigger sexual reproduction (conjugation) under starvation conditions. Gamone 1 is a glycoprotein secreted by type I cells, and gamone 2 is a tryptophan (Trp)-derivative compound secreted by type II cells. Both gamones stimulate complementary mating type cells to promote each gamone production and induce pair formation. To elucidate the biosynthetic pathway of gamone 2, we investigated the enzymes involved in the pathway and the specificity of the enzymes. An RNA-seq analysis revealed that Blepharisma stoltei (Heterotrichea) possesses four indoleamine 2,3-dioxygenase (IDO) genes showing distinct expression patterns. Along with results from real-time PCR, these findings demonstrated that each IDO gene has different expression patterns that depend on the cellular conditions. Expression of IDO-I was correlated with the intensity of gamone 2 expression, and the recombinant IDO-I protein showed catalytic activity for 5-hydroxy-L-Trp (5-HTP) but very weak activity for L-Trp. Our results indicate that IDO-I is an enzyme evolutionary specialized to gamone 2 production in Blepharisma, and that the biosynthetic pathway for gamone 2 uses 5-HTP as an intermediate. (C) 2017 Elsevier GmbH. All rights reserved., 2017年12月, PROTIST, 168 (6), 686 - 696, doi;web_of_science

    研究論文(学術雑誌)

  • Low- and High-LET Ionizing Radiation Induces Delayed Homologous Recombination that Persists for Two Weeks before Resolving

    Christopher P. Allen; Hirokazu Hirakawa; Nakako Izumi Nakajima; Sophia Moore; Jingyi Nie; Neelam Sharma; Mayumi Sugiura; Yuko Hoki; Ryoko Araki; Masumi Abe; Ryuichi Okayasu; Akira Fujimori; Jac A. Nickoloff

    Genome instability is a hallmark of cancer cells and dysregulation or defects in DNA repair pathways cause genome instability and are linked to inherited cancer predisposition syndromes. Ionizing radiation can cause immediate effects such as mutation or cell death, observed within hours or a few days after irradiation. Ionizing radiation also induces delayed effects many cell generations after irradiation. Delayed effects include hypermutation, hyper-homologous recombination, chromosome instability and reduced clonogenic survival (delayed death). Delayed hyperrecombination (DHR) is mechanistically distinct from delayed chromosomal instability and delayed death. Using a green fluorescent protein (GFP) direct repeat homologous recombination system, time-lapse microscopy and colony-based assays, we demonstrate that DHR increases several-fold in response to low-LET X rays and high-LET carbon-ion radiation. Time-lapse analyses of DHR revealed two classes of recombinants not detected in colony-based assays, including cells that recombined and then senesced or died. With both low-and high-LETradiation, DHR was evident during the first two weeks postirradiation, but resolved to background levels during the third week. The results indicate that the risk of radiation-induced genome destabilization via DHR is time limited, and suggest that there is little or no additional risk of radiation-induced genome instability mediated by DHR with high-LET radiation compared to low-LET radiation. (C) 2017 by Radiation Research Society, 2017年07月, RADIATION RESEARCH, 188 (1), 82 - 93, doi;web_of_science

    研究論文(学術雑誌)

  • Rapid response to nutrient depletion on the expression of mating pheromone, gamone 1, in Blepharisma japonicum.

    Sugiura M; Yamanaka M; Suzaki T; Harumoto T

    2016年, Jpn. J. Protozool., 49 (1,2), 27 - 36, doi

    研究論文(学術雑誌)

  • Two Possible Barriers Blocking Conjugation Between Different Megakaryotypes of Blepharisma

    Mayumi Kobayashi; Mamiko Miura; Mari Takusagawa; Mayumi Sugiura; Terue Harumoto

    We investigated mating pair formation between three Blepharisma species Blepharisma undulans, Blepharisma japonicum, and Blepharisma stoltei to determine whether their respective gamones (mating pheromones) effectively induce mating pairs between different species. Cell-free fluid from type II cells (CFF2) of B. undulans (megakaryotype II) induced pairing of B. japonicum and B. stoltei type I cells (megakaryotype IV), and CFF2 of B. japonicum and B. stoltei induced pairing of B. undulans type I cells. Cell-free fluid from B. undulans type I cells (CFF1) did not induce pairing of B. japonicum and B. stoltei type II cells, and CFF1 of B. japonicum and B. stoltei failed to induce pairing of B. undulans. CFF1 from B. japonicum and B. stoltei mutually induced pairing, as previously reported. These results indicate that gamone 2 is common among megakaryotypes II and IV, and that gamone 1 appears to be at least megakaryotype-specific. When cells belonging to megakaryotypes II and IV are separately pre-treated with effective gamones and mixed, mating pairs between megakaryotypes rarely form. Taken together, these results suggest at least two barriers, a gamone and a factor involved in pair formation, that prevent conjugation between different megakaryotypes of Blepharisma., 2015年01月, ZOOLOGICAL SCIENCE, 32 (1), 53 - 61, doi;web_of_science

    研究論文(学術雑誌)

  • Induced Pluripotent Stem Cell Generation-Associated Point Mutations Arise during the Initial Stages of the Conversion of These Cells

    Mayumi Sugiura; Yasuji Kasama; Ryoko Araki; Yuko Hoki; Misato Sunayama; Masahiro Uda; Miki Nakamura; Shunsuke Ando; Masumi Abe

    A large number of point mutations have been identified in induced pluripotent stem cell (iPSC) genomes to date. Whether these mutations are associated with iPSC generation is an important and controversial issue. In this study, we approached this critical issue in different ways, including an assessment of iPSCs versus embryonic stem cells (ESCs), and an investigation of variant allele frequencies and the heterogeneity of point mutations within a single iPSC clone. Through these analyses, we obtained strong evidence that iPSC-generation-associated point mutations occur frequently in a transversion-predominant manner just after the onset of cell lineage conversion. The heterogeneity of the point mutation profiles within an iPSC clone was also revealed and reflects the history of the emergence of each mutation. Further, our results suggest a possible approach for establishing iPSCs with fewer point mutations., 2014年01月, STEM CELL REPORTS, 2 (1), 52 - 63, doi;web_of_science

    研究論文(学術雑誌)

  • Negligible immunogenicity of terminally differentiated cells derived from induced pluripotent or embryonic stem cells

    Ryoko Araki; Masahiro Uda; Yuko Hoki; Misato Sunayama; Miki Nakamura; Shunsuke Ando; Mayumi Sugiura; Hisashi Ideno; Akemi Shimada; Akira Nifuji; Masumi Abe

    The advantages of using induced pluripotent stem cells (iPSCs) instead of embryonic stem (ES) cells in regenerative medicine centre around circumventing concerns about the ethics of using ES cells and the likelihood of immune rejection of ES-cell-derived tissues(1,2). However, partial reprogramming and genetic instabilities in iPSCs(3-6) could elicit immune responses in transplant recipients even when iPSC-derived differentiated cells are transplanted. iPSCs are first differentiated into specific types of cells in vitro for subsequent transplantation. Although model transplantation experiments have been conducted using various iPSC-derived differentiated tissues(7-10) and immune rejections have not been observed, careful investigation of the immunogenicity of iPSC-derived tissue is becoming increasingly critical, especially as this has not been the focus of most studies done so far. A recent study reported immunogenicity of iPSC- but not ES-cell-derived teratomas(11) and implicated several causative genes. Nevertheless, some controversy has arisen regarding these findings(12). Here we examine the immunogenicity of differentiated skin and bone marrow tissues derived from mouse iPSCs. To ensure optimal comparison of iPSCs and ES cells, we established ten integration-free iPSC and seven ES-cell lines using an inbred mouse strain, C57BL/6. We observed no differences in the rate of success of transplantation when skin and bone marrow cells derived from iPSCs were compared with ES-cell-derived tissues. Moreover, we observed limited or no immune responses, including T-cell infiltration, for tissues derived from either iPSCs or ES cells, and no increase in the expression of the immunogenicity-causing Zg16 and Hormad1 genes in regressing skin and teratoma tissues. Our findings suggest limited immunogenicity of transplanted cells differentiated from iPSCs and ES cells., 2013年02月, NATURE, 494 (7435), 100 - 104, doi;web_of_science

    研究論文(学術雑誌)

  • Alternative Gene Expression in Type I and Type II Cells May Enable further Nuclear Changes during Conjugation of Blepharisma japonicum

    Mayumi Sugiura; Yuri Tanaka; Toshinobu Suzaki; Terue Harumoto

    In contrast to most ciliates, meiosis and successive nuclear changes during conjugation occur only in heterotypic pairs in Blepharisma. It has been suggested that homotypic pairs are ready for conjugation, but lack a trigger to initiate the nuclear changes, and the conjugation process is arrested before the onset of meiosis. To explore the possible nature of the trigger, we previously identified the genes BjCdk1 (homologous to cdk1/cdc2), Bj4HPPD (4-hydroxy-phenylpyruvate dioxygenase) and BjCks (cyclin dependent kinase regulatory subunit) whose expression is up-regulated in gamone1-treated type II cells. In this study, we investigated the molecular structures of these three genes, and compared their expression patterns in homotypic and heterotypic pairs, finding remarkable differences. BjCdk1, Bj4HPPD and BjCks were expressed specifically in gamone1-treated type II cells, but not in gamone2-treated type I cells. In heterotypic pairs, the expression of these genes stayed at the same level or gradually decreased throughout the entire process of conjugation, but it rapidly decreased and ceased after 10 hours in homotypic pairs. These results indicate that some genes are expressed in a mating-type specific manner. Alternative gene expression in mating type I and type II cells and merging of individual factors in a heterotypic pair may induce nuclear changes including meiosis. (C) 2011 Elsevier GmbH. All rights reserved., 2012年03月, PROTIST, 163 (2), 204 - 216, doi;web_of_science

    研究論文(学術雑誌)

  • Crucial Role of C-Myc in the Generation of Induced Pluripotent Stem Cells

    Ryoko Araki; Yuko Hoki; Masahiro Uda; Miki Nakamura; Yuko Jincho; Chihiro Tamura; Misato Sunayama; Shunsuke Ando; Mayumi Sugiura; Mitsuaki A. Yoshida; Yasuji Kasama; Masumi Abe

    c-Myc transduction has been considered previously to be nonessential for induced pluripotent stem cell (iPSC) generation. In this study, we investigated the effects of c-Myc transduction on the generation of iPSCs from an inbred mouse strain using a genome integration-free vector to exclude the effects of the genetic background and the genomic integration of exogenous genes. Our findings reveal a clear difference between iPSCs generated using the four defined factors including c-Myc (4F-iPSCs) and those produced without c-Myc (3F-iPSCs). Molecular and cellular analyses did not reveal any differences between 3F-iPSCs and 4F-iPSCs, as reported previously. However, a chimeric mice formation test indicated clear differences, whereby few highly chimeric mice and no germline transmission was observed using 3F-iPSCs. Similar differences were also observed in the mouse line that has been widely used in iPSC studies. Furthermore, the defect in 3F-iPSCs was considerably improved by trichostatin A, a histone deacetyl transferase inhibitor, indicating that c-Myc plays a crucial role in iPSC generation through the control of histone acetylation. Indeed, low levels of histone acetylation were observed in 3F-iPSCs. Our results shed new light on iPSC generation mechanisms and strongly recommend c-Myc transduction for preparing high-quality iPSCs. STEM CELLS 2011;29:1362-1370, 2011年09月, STEM CELLS, 29 (9), 1362 - 1370, doi;web_of_science

    研究論文(学術雑誌)

  • Behavioural changes induced by the conjugation-inducing pheromones, gamone 1 and 2, in the ciliate Blepharisma japonicum

    Mayumi Sugiura; Hiromi Shiotani; Toshinobu Suzaki; Terue Harumoto

    Preconjugant interactions between complementary mating-type cells in ciliates occur before sexual reproduction. The interactions include retardation of swimming behaviour, courtship dancing, chemoattraction, nuclear activation, cell division, or cell agglutination, depending on ciliate species. In Blepharisma japonicum, chemoattraction of mating-type I by mating-type II has been reported previously. It has been shown that chemoattraction here is caused by a conjugation-inducing substance called gamone 2 secreted by mating-type II cells. In this study, we show that mating-type II cells accumulate near the site where gamone 1 secreted by mating-type I cells is present at a high concentration. We also show that the behaviour of individual cells changes when exposed to the complementary mating-type gamone; cells begin to rotate and swim slowly, thus shortening their minimum path length (final displacement of a cell from its origin). These results suggest that gamones 1 and 2 induce behavioural changes in type II and I cells, respectively, and that gamone-stimulated cells may accumulate at the site with the highest activity of the complementary gamone, after repetition of swimming changes in the gradient of gamone concentration. This reciprocal induction of the changes in behaviour may increase the probability of sexual encounters for conjugation. (C) 2010 Elsevier GmbH. All rights reserved., 2010年05月, EUROPEAN JOURNAL OF PROTISTOLOGY, 46 (2), 143 - 149, doi;web_of_science

    研究論文(学術雑誌)

  • Synthesis of fluorescent molecular probes specific for the receptor of blepharismone, a mating-inducing pheromone of the ciliate Blepharisma japonicum

    Yoshiyuki Uruma; Mayumi Sugiura; Terue Harumoto; Yoshinosuke Usuki; Hideo Iio

    Blepharismone (gamone 2) is a mating-inducing pheromone of the ciliate Blepharisma japonicum. N-Pyrenylbutyryl-blepharismone and N-biphenylacetyl-blepharismone, which are fluorescent derivatives of blepharismone, were synthesized as molecular probes for the gamone 2 receptor. Further, we proved that they have inhibitory activities against the blepharismone-induced monotypic pairing of B. japonicum. Published by Elsevier Ltd., 2007年02月, BIOORGANIC & MEDICINAL CHEMISTRY, 15 (4), 1622 - 1627, doi;web_of_science

    研究論文(学術雑誌)

  • Cdk1 and cks gene homologs are transcriptionally activated during induction of conjugating pairs in mating-type II cells of the ciliate Blepharisma japonicum

    Tanaka Y; Sugiura M; Harumoto T

    2007年, Jpn. J. Protozool., 40 (2), 131 - 138, doi

    研究論文(学術雑誌)

  • Developmentally and environmentally regulated expression of gamone 1: the trigger molecule for sexual reproduction in Blepharisma japonicum

    M Sugiura; S Kawahara; H Iio; T Harumoto

    Sexual reproduction (conjugation) in protozoan ciliates is induced by specific cell-cell interactions between cells of complementary mating types. The ancestral ciliate Blepharisma japonicum has two mating types, I and II. The substances that act as signaling molecules in this extracellular interaction for conjugation are called gamones. The glycoprotein gamone 1, produced by mating type I cells, is a key factor that triggers this interaction. We have previously isolated gamone 1 and determined its complete amino acid sequence. To elucidate the mechanism of initiation of conjugation in ciliates, we investigated the transcription of the gamone I gene and found that it is controlled by various internal and external factors. The gamone I gene transcript appeared specifically when sexually mature mating type I cells were starved. It was not detected in immature cells, mating type 11 cells or proliferating cells. The level of transcription was markedly increased in type I cells when they were stimulated with gamone 2, which is secreted by type H cells. This is the first report that the transcription of gamone genes in ciliates is strictly regulated by developmental and environmental factors. This study suggests that the onset of transcription of gamone I is linked to the switching mechanism that converts mitotically proliferating cells to differentiated preconjugants, the mechanism of differentiation from immature to mature cells in clonal development, and the mechanism that ensures mating type-specific gene silencing., 2005年06月, JOURNAL OF CELL SCIENCE, 118 (12), 2735 - 2741, doi;web_of_science

    研究論文(学術雑誌)

  • ND7 gene involved in the membrane fusion in regulated exocytosis is conserved in three species of Paramecium

    Harumoto T; Mizoguchi M; Sugiura M

    2004年, Jpn. J. Protozool., 37 (2), 151 - 158

    研究論文(学術雑誌)

  • Identification, characterization, and complete amino acid sequence of the conjugation-inducing glycoprotein (blepharmone) in the ciliate Blepharisma japonicum

    M Sugiura; T Harumoto

    Conjugation in Blepharisma japonicum is induced by interaction between complementary mating-types I and II, which excrete blepharmone (gamone 1) and blepharismone (gamone 2), respectively. Gamone 1 transforms type II cells such that they can unite, and gamone 2 similarly transforms type I cells. Moreover, each gamone promotes the production of the other gamone. Gamone 2 has been identified as calcium-3-(2'-formylamino-5'-hydroxy-benzoyl) lactate and has been synthesized chemically. Gamone 1 was isolated and characterized as a glycoprotein of 20-30 kDa containing 175 amino acids and 6 sugars. However, the amino acid sequence and arrangement of sugars in this gamone are still unknown. To determine partial amino acid sequences of gamone 1, we established a method of isolation based on the finding that this glycoprotein can be concentrated by a Con A affinity column. Gamone 1 is extremely unstable and loses its biological activity once adsorbed to any of the columns that we tested. By using a Con A affinity column and native PAGE, we detected a 30-kDa protein corresponding to gamone 1 activity and determined the partial amino acid sequences of the four peptides. To isolate gamone 1 cDNA, we isolated mRNA from mating-type I cells stimulated by synthetic gamone 2 and then performed rapid amplification of cDNA ends procedures by using gene-specific primers and cloned DNA of gamone 1. The cDNA sequence contains an ORF of 305 amino acids and codes a possibly novel protein. We also estimated the arrangement of sugars by comparing the affinity to various lectin columns., 2001年12月, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 98 (25), 14446 - 14451, doi;web_of_science

    研究論文(学術雑誌)

  • Induced pluripotent stem cell generation-associated point mutations.

    Araki R; Sugiura M; Hoki Y; Sunayama M; Nakamura M; Kasama Y; Abe M

    2015年, Inflammation and Regeneration, 35 (5), 226 - 232, doi

  • 繊毛虫ブレファリズマにおける接合誘導物質ガモン1抗体を用いた受容体の探索

    杉浦 真由美; 春本 晃江; 洲崎 敏伸

    2009年, 原生動物学雑誌, 42: 34-35.

    研究論文(学術雑誌)

MISC

  • iPS化に伴う点突然変異

    荒木良子; 杉浦真由美; 笠間康次; 安倍真澄

    2015年, 日本臨牀, 73 (増刊号5), 55 - 61

    記事・総説・解説・論説等(学術雑誌)

  • ブレファリズマの接合

    春本晃江; 杉浦真由美

    2003年, 原生動物学雑誌, 36 (2), 147 - 172

    記事・総説・解説・論説等(学術雑誌)

  • Conjugation in Blepharisma japonicum

    Harumoto T; Sugiura M

    2003年, 36 (2), 147 - 172

  • Cell-cell interaction prior to conjugation in ciliates

    Terue Harumoto; Mayumi Sugiura

    ZOOLOGICAL SOC JAPAN, 2006年12月, ZOOLOGICAL SCIENCE, 23 (12), 1139 - 1139, web_of_science

    研究発表ペーパー・要旨(国際会議)

  • Genes specifically expressed during the induction of conjugation in mating type II cells of the ciliate Blepharisma japonicum

    Yuri Tanaka; Mayumi Sugiura; Terue Harumoto

    ZOOLOGICAL SOC JAPAN, 2006年12月, ZOOLOGICAL SCIENCE, 23 (12), 1161 - 1161, web_of_science

    研究発表ペーパー・要旨(国際会議)

  • Cloning of the genes which specifically expressed during the induction of conjugation in type II cells of Blepharisma japonicum

    Yuri Tanaka; Mayumi Sugiura; Terue Harumoto

    ZOOLOGICAL SOC JAPAN, 2004年12月, ZOOLOGICAL SCIENCE, 21 (12), 1277 - 1277, web_of_science

    研究発表ペーパー・要旨(国際会議)

  • Developmentally and environmentally regulated expression of mating pheromone in the ciliate Blepharisma japonicum

    Mayumi Sugiura; Seiko Kawahara; Terue Harumoto

    ZOOLOGICAL SOC JAPAN, 2004年12月, ZOOLOGICAL SCIENCE, 21 (12), 1277 - 1277, web_of_science

    研究発表ペーパー・要旨(国際会議)

  • Identification of the upstream sequences of gamone 1 gene in the ciliate Blepharisma japonicum

    Risa Takami; Mayumi Sugiura; Terue Harumoto

    ZOOLOGICAL SOC JAPAN, 2004年12月, ZOOLOGICAL SCIENCE, 21 (12), 1277 - 1277, web_of_science

    研究発表ペーパー・要旨(国際会議)

講演・口頭発表等

  • 比較的原始的な繊毛虫における有性生殖誘導の鍵物質:フェロモンの多様性

    杉浦真由美

    第43回日本分子生物学会年会・フォーラム:有性生殖物語・原生生物編「生態,フェロモン,クロマチン,そして進化」, 2020年12月02日, 2020年12月02日

    口頭発表(招待・特別)

  • 原生生物繊毛虫における有性生殖の多様性を探る

    杉浦真由美

    日本動物学会大会第91回大会・本部企画シンポジウム「第1回茗原眞路子研究奨励助成記念シンポジウム」, 2020年09月04日, 2020年09月04日

    口頭発表(招待・特別)

  • Blepharisma hyalinum のガモン1相同遺伝子の全長配列とその特徴

    小森美沙希; 杉野亜優; 春本晃江; 杉浦真由美

    日本原生生物学会第52回大会, 2019年10月25日, 2019年10月25日

    ポスター発表

  • 繊毛虫ブレファリズマにおけるトリプトファン由来生理活性物質の接合への影響およびガモン2受容体の局在解析に向けた蛍光プローブの検討

    熊谷歩乃佳; 吉川千晶; 臼杵克之助; 春本晃江; 杉浦真由美

    日本原生生物学会第52回大会, 2019年10月25日, 2019年10月25日

    ポスター発表

  • Blepharisma stolteiにおけるジャイアント形成に伴う遺伝子発現の変化

    大北千紗; 春本晃江; 杉浦真由美

    日本原生生物学会第52回大会, 2019年10月25日, 2019年10月25日

    ポスター発表

  • 繊毛虫ブレファリズマにおける網羅的解析を用いたガモン1受容体候補の探索

    山根菜摘; 洲崎敏伸; 春本晃江; 杉浦真由美

    日本原生生物学会第52回大会, 2019年10月25日, 2019年10月25日

    ポスター発表

  • IDO enzyme involved in the biosynthesis of mating pheromone (Gamone) in the ciliate Blepharisma

    Mayumi Sugiura; Hajime J. Yuasa; Terue Harumoto

    VIII European Congress of Protistology – ISOP joint meeting, 2019年07月28日, 2019年07月28日, true

    ポスター発表

  • 繊毛虫Blepharismaにおけるガモン2生合成に関わるTMO相同遺伝子の探索

    佐藤恵那; 杉浦真由美; 春本晃江

    日本原生生物学会第51回大会, 2018年10月, false

    ポスター発表

  • 繊毛虫ブレファリズマの数種における凍結保存の試み

    佐々木愛澄; 杉浦真由美; 春本晃江

    日本原生生物学会第51回大会, 2018年10月, false

    ポスター発表

  • 繊毛虫Blepharismaにおけるジャイアント形成機構の解明に向けて‐新たな繊毛形成に関わる遺伝子の探索とその発現解析‐

    大北千紗; 春本晃江; 杉浦真由美

    日本原生生物学会第51回大会, 2018年10月, false

    ポスター発表

  • 繊毛虫Blepharisma stolteiにおけるジャイアントの形態的特徴と形成過程

    井坂友紀; 杉浦真由美; 春本晃江

    日本原生生物学会第51回大会, 2018年10月, false

    ポスター発表

  • Morphological Traits and Formation Process of Giant Cells in the Ciliate Blepharisma stoltei

    Yuki Isaka; Mayumi Sugiura; Terue Harumoto

    Joint meeting of the Korean Society of Protistologists and Japan Society of Protistology, 2018年07月, true

    ポスター発表

  • Toward the elucidation of the giant formation mechanism in ciliate Blepharisma: Identification of genes that may be involved in the formation of new cilia

    Kazusa Okita; Terue Harumoto; Mayumi Sugiura

    Joint meeting of the Korean Society of Protistologists and Japan Society of Protistology, 2018年07月, true

    ポスター発表

  • IDO enzyme involved in the biosynthesis of mating pheromone in the ciliate Blepharisma

    Mayumi Sugiura; Hajime J. Yuasa; Terue Harumoto

    Joint meeting of the Korean Society of Protistologists and Japan Society of Protistology, 2018年07月, true

    ポスター発表

  • トリプトファン分解酵素の分子進化の新局面 IX

    湯浅創; 杉浦真由美; 春本晃江

    2017年度生命科学系学会合同年次大会, 2017年12月, false

  • Blepharisma hyalinumの表層顆粒の機能とその内容物の毒性

    田村望; 杉浦真由美; 春本晃江

    第50回日本原生生物学会大会・第1回日本共生生物学会大会合同大会, 2017年11月, false

    ポスター発表

  • 繊毛虫Blepharisma属における異種間接合を防ぐ仕組みの解明に向けて

    杉野亜優; 杉浦真由美; 春本晃江

    第50回日本原生生物学会大会・第1回日本共生生物学会大会合同大会, 2017年11月, false

    ポスター発表

  • 繊毛虫ブレファリズマにおける凍結保存の試み

    佐々木愛澄; 杉浦真由美; 春本晃江

    第50回日本原生生物学会大会・第1回日本共生生物学会大会合同大会, 2017年11月, false

    ポスター発表

  • 繊毛虫Blepharisma stolteiにおけるジャイアントの形態的特徴

    井坂友紀; 杉浦真由美; 春本晃江

    第50回日本原生生物学会大会・第1回日本共生生物学会大会合同大会, 2017年11月, false

    ポスター発表

  • ブレファリズマにおけるジャイアントの形成と生存戦略

    尾野 優奈; 杉浦 真由美; 春本 晃江

    日本動物学会第88回大会, 2017年09月, false

    ポスター発表

  • ブレファリズマの環境応答:共食い・接合・休眠

    杉浦真由美

    日本動物学会第88回大会、シンポジウム「原生生物の魅力と研究材料としての有用性」, 2017年09月, false

    口頭発表(招待・特別)

  • Giant Formation and Surviving Strategy in Blepharisma

    Terue Harumoto; Yuna Ono; Mayumi Sugiura

    XV International Congress of Protistology-ICOP, 2017年07月, true

    ポスター発表

  • Blepharisma hyalinumの表層顆粒の機能の探索

    田村望; 杉浦真由美; 春本晃江

    日本原生生物学会第49回大会, 2016年10月, false

    口頭発表(一般)

  • 繊毛虫Blepharismaの異なるmegakaryotype間での異種間接合

    杉野亜優; 杉浦真由美; 春本晃江

    日本原生生物学会第49回大会, 2016年10月, false

    口頭発表(一般)

  • 繊毛虫ブレファリズマにおいてガモン2生合成に関与する新規IDOの同定

    杉浦真由美; 湯浅創; 春本晃江

    日本原生生物学会第49回大会, 2016年10月, false

    口頭発表(一般)

  • ブレファリズマのジャイアント形成の意義と形成条件の検討

    尾野優奈; 杉浦真由美; 春本晃江

    日本原生生物学会第48回大会, 2015年11月, false

    口頭発表(一般)

  • Blepharisma hyalinum の分類の再検討

    小林真弓; 杉浦真由美; 春本晃江

    日本原生生物学会第48回大会, 2015年11月, false

    口頭発表(一般)

  • ブレファリズマの性成熟過程における接合型発現パターンと遺伝子発現解析

    杉浦真由美; 春本晃江

    日本原生生物学会第48回大会, 2015年11月, false

    口頭発表(一般)

  • 繊毛虫ブレファリズマにおける交配フェロモンの多様性と種分化

    春本晃江; 小林真弓; 杉浦真由美; 篠原きよの; 田草川真里

    日本動物学会第86回大会, 2015年09月, false

    口頭発表(一般)

  • Mating pheromone gamone 1 plays an important role in speciation of the ciliate Blepharisma

    Mayumi Kobayashi; Yui Nishihara; Mamiko Miura; Mari Takusagawa; Mayumi Sugiura; Terue Harumoto

    Ciliate Molecular Biology Conference, 2015年07月, true

    ポスター発表

  • Mating type expression during sexual maturation in Blepharisma stoltei

    Mayumi Sugiura; Terue Harumoto

    Ciliate Molecular Biology Conference, 2015年07月, true

    ポスター発表

  • iPS樹立初期過程には多くのpoint mutationが生じる

    藤森(法喜)ゆう子; 杉浦真由美; 笠間康次; 砂山美里; 宇田昌広; 中村美樹; 荒木良子; 安倍真澄

    第37回日本分子生物学会年会, 2014年11月, false

  • Molecular mechanism of induction of sexual reproduction in the ciliates

    杉浦真由美

    第37回日本分子生物学会年会、ワークショップ(原生生物~モデル生物としての大いな る可能性を探る~), 2014年11月, false

  • 繊毛虫Blepharisma属における交配フェロモンgamone1の多様性と種分化

    小林真弓; 田草川真理; 杉浦真由美; 春本晃江

    日本原生生物学会第47回大会, 2014年10月, false

  • ブレファリズマの交配フェロモン(ガモン1)の糖鎖構造とその役割

    春本晃江; 山岸由和; 岩﨑祥子; 杉浦真由美; 小林真弓; 飯尾英夫

    日本原生生物学会第47回大会, 2014年10月, false

  • ブレファリズマにおけるジャイアント形成要因の検討と口部構造の観察

    尾野優奈; 杉浦真由美; 春本晃江

    日本動物学会第85回大会, 2014年09月, false

  • 繊毛虫ブレファリズマの分類の再検討

    小林真弓; 杉浦真由美; 春本晃江

    日本動物学会第85回大会, 2014年09月, false

  • 繊毛虫 Blepharisma における交配フェロモンの生合成にIDO は関与するか

    亀岡里江子; 篠原きよの; 杉浦真由美; 春本晃江

    日本動物学会第85回大会, 2014年09月, false

  • ES cells vs. iPS cells: Lineage conversion-associated point mutations

    Yuko Hoki; Mayumi Sugiura; Yasuji Kasama; Misato Sunayama; Masahiro Uda; Miki Nakamura; Shunsuke Ando; Ryoko Araki; Masumi Abe

    International Society for Stem Cell Research-ISSCR 12th Annual meeting, 2014年06月, true

  • Point mutations in ES cells

    砂山美里; 杉浦真由美; 法喜ゆう子; 笠間康次; 宇田昌広; 中村美樹; 安藤俊輔; 荒木良子; 安倍真澄

    第36回日本分子生物学会年会, 2013年12月, false

  • iPS cells generation-associated point mutations

    荒木良子; 杉浦真由美; 笠間康次; 砂山美里; 宇田昌広; 安藤俊輔; 中村美樹; 法喜ゆう子; 安倍真澄

    第36回日本分子生物学会年会, 2013年12月, false

  • Acceleration of the histone acetylation in pluripotent iPS cells

    杉浦真由美; 法喜ゆう子; 砂山美里; 宇田昌広; 荒木良子; 安倍真澄

    第36回日本分子生物学会年会, 2013年12月, false

  • 繊毛虫Blepharismaの異種間接合対において核変化は起こりうるのか

    山田真央; 小林真弓; 杉浦真由美; 春本晃江

    日本原生動物学会第46回大会, 2013年11月, false

  • 細胞密度がブレファリズマのジャイアント形成に与える影響

    尾野優奈; 杉浦真由美; 春本晃江

    日本原生動物学会第46回大会, 2013年11月, false

  • 繊毛虫ブレファリズマの異種間接合を妨げる要因

    小林真弓; 杉浦真由美; 春本晃江

    日本動物学会第84回大会, 2013年09月, false

  • 接合誘導物質ガモン2 に類似するアミノ酸が繊毛虫Blepharismaのガモン2 活性に 与える影響

    篠原きよの; 春本晃江; 杉浦真由美

    日本動物学会第84回大会, 2013年09月, false

  • Two barriers prevent interspecific mating pair formation in Blepharisma

    Mayumi Kobayashi; Mayumi Sugiura; Terue Harumoto

    FASEB Summer Research Conferences, Ciliate Molecular Biology, 2013年07月, true

  • iPS cells generation-associated point mutations

    Ryoko Araki; Mayumi Sugiura; Yasuji Kasama; Misato Sunayama; Masahiro Uda; Miki Nakamura; Shunsuke Ando; Yuko Hoki; Masumi Abe

    International Society for Stem Cell Research-ISSCR 11th Annual Meeting, 2013年06月, true

  • Point mutations in ES cells

    Yuko Hoki; Mayumi Sugiura; Yasuji Kasama; Misato Sunayama; Masahiro Uda; Miki Nakamura; Shunsuke Ando; Ryoko Araki; Masumi Abe

    International Society for Stem Cell Research-ISSCR 11th Annual Meeting, 2013年06月, true

  • A comparison between iPSCs and ESCs reveals that genome reprogramming during iPSC generation causes transversion-predominant point mutations

    杉浦真由美; 笠間康次; 藤森ゆう子; 宇田昌広; 中村美樹; 安藤俊輔; 砂山美里; 荒木良子; 安倍真澄

    第35回日本分子生物学会年会, 2012年12月, false

  • A comparison between immunogenicity of iPSCs and of ESCs

    Ryoko Araki; Masahiro Uda; Yuko Hoki-Fujimori; Miki Nakamura; Shunsuke Ando; Misato Sunayama; Mayumi Sugiura; Hisashi Ideno; Akemi Shimada; Akira Nifuji; Masumi Abe

    International Society for Stem Cell Research-ISSCR 10th Annual Meeting, 2012年06月, true

  • A Comparison between iPSCs and ESCs reveals reprogramming-associated point mutations

    Masumi Abe; Mayumi Sugiura; Yasuji Kasama; Yuko Hoki-Fujimori; Masahiro Uda; Miki Nakamura; Shunsuke Ando; Misato Sunayama; Ryoko Araki

    International Society for Stem Cell Research-ISSCR 10th Annual Meeting, 2012年06月, true

  • c-MycはiPS細胞の多能性獲得において重要な役割を担っている

    宇田昌広; 荒木良子; 藤森ゆう子; 中村美樹; 安藤俊輔; 神長祐子; 杉浦真由美; 砂山美里; 笠間康次; 安倍真澄

    第34回日本分子生物学会年会, 2011年12月, false

  • マウスiPS細胞およびES細胞における点突然変異のゲノムワイド解析

    杉浦真由美; 荒木良子; 笠間康次; 砂山美里; 安藤俊輔; 藤森ゆう子; 宇田昌広; 中村美樹; 安倍真澄

    第34回日本分子生物学会年会, 2011年12月, false

  • Function of mating pheromones, Gamone 1 and 2, in the ciliated protozoa Blepharisma japonicum

    Mayumi Sugiura; Yuri Tanaka; Shoko Iwasaki; Toshinobu Suzaki; Terue Harumoto

    XIII International Congress of Protistology-ICOP, 2009年08月, true

  • Mating interaction through Gamones in Blepharisma japonicum

    Mayumi Sugiura; Terue Harumoto

    FASEB Summer Research Conferences, Ciliate Molecular Biology, 2009年07月, true

  • 原生動物繊毛虫ブレファリズマの交配フェロモンとしてのトリプトファン誘導体

    杉浦真由美; 洲崎敏伸; 飯尾英夫; 岩﨑祥子; 春本晃江

    日本トリプトファン研究会第30回学術集会, 2008年12月, false

  • 原生生物繊毛虫ブレファリズマにおける交配フェロモンの機能

    杉浦真由美; 田中悠里; 岩﨑祥子; 吉村千代; 沢田奈穂子; 洲崎敏伸; 春本晃江

    第31回日本分子生物学会年会、第81回日本生化学会大会合同大会, 2008年12月, false

  • 繊毛虫ブレファリズマにおける接合誘導物質ガモン1抗体を用いた受容体の探索

    杉浦真由美; 春本晃江; 洲崎敏伸

    日本原生動物学会第41回大会, 2008年11月, false

  • 繊毛虫Blepharisma japonicumにおける接合誘導物質ガモン1の糖鎖の役割

    岩﨑祥子; 杉浦真由美; 春本晃江

    日本原生動物学会第41回大会, 2008年11月, false

  • ブレファリズマのII型細胞で接合誘導時特異的に発現するcdk1, cks遺伝子の分子的特徴

    田中悠里; 杉浦真由美; 春本晃江

    日本原生動物学会第40回大会, 2007年11月, false

  • 繊毛虫ブレファリズマにおける接合誘導物質ガモン1の糖鎖の研究-糖鎖は接合誘導活性に必要か?-

    岩﨑祥子; 杉浦真由美; 春本晃江

    日本原生動物学会第40回大会, 2007年11月, false

  • 強制飢餓によるガモン1の発現誘導-発現開始と飢餓の関連性-

    山中美果; 杉浦真由美; 田中悠里; 春本晃江

    日本原生動物学会第40回大会, 2007年11月, false

  • 繊毛虫Blepharisma stolteiとB. americanumにおけるガモン1相同遺伝子の単離と配列比較

    三浦満美子; 杉浦真由美; 春本晃江

    日本動物学会第78回大会, 2007年09月, false

  • Specifically regulated expression of gamone 1: the trigger molecule for conjugation in Blepharisma japonicum

    Mayumi Sugiura; Seiko Kawahara; Kaori Teramoto; Kanae Kobayashi; Risa Takami; Mika Yamanaka; Yuri Tanaka; Terue Harumoto

    FASEB Summer Research Conferences, Ciliate Molecular Biology, 2007年07月, true

  • Gene expression toward conjugation in Blepharisma japonicum

    Terue Harumoto; Mayumi Sugiura; Yuri Tanaka

    FASEB Summer Research Conferences, Ciliate Molecular Biology, 2007年07月, true

  • Genes transcriptionally activated during induction of conjugating pairs in mating-type II cells of the ciliate Blepharisma japonicum

    Yuri Tanaka; Mayumi Sugiura; Terue Harumoto

    FASEB Summer Research Conferences, Ciliate Molecular Biology, 2007年07月, true

  • 繊毛虫Blepharisma stolteiにおけるガモン1相同遺伝子の検出と配列決定

    三浦満美子; 杉浦真由美; 春本晃江

    日本原生動物学会第39回大会, 2006年11月, false

  • 繊毛虫ブレファリズマのII型細胞の接合誘導時に特異的に発現する遺伝子

    田中悠里; 杉浦真由美; 春本晃江

    日本動物学会第77回大会, 2006年09月, false

  • 繊毛虫ブレファリズマで接合誘導時に発現する遺伝子の研究

    杉浦真由美; 田中悠里; 久保陽子; 春本晃江

    日本原生動物学会第38回大会, 2005年10月, false

  • Gene expression involved in early stage of conjugation in Blepharisma japonicum

    Mayumi Sugiura; Yuri Tanaka; Risa Takami; Seiko Kawahara; Terue Harumoto

    FASEB Summer Research Conferences, Ciliate Molecular Biology, 2005年08月, true

  • Specific expression of mating pheromone, gamone 1 in the ciliate Blepharisma japonicum

    Mayumi Sugiura

    XII International Congress of Protozoology-ICOP, 2005年07月, true

  • Genes which specifically expressed during the induction of conjugation in mating type II cells of the ciliate Blepharisma japonicum

    Yuri Tanaka; Mayumi Sugiura; Terue Harumoto

    XII International Congress of Protozoology-ICOP, 2005年07月, true

  • 原生生物繊毛虫ブレファリズマにおける性フェロモンの同定と発現制御

    杉浦真由美; 高見梨沙; 川原聖子; 春本晃江

    第27回日本分子生物学会年会, 2004年12月, false

  • 繊毛虫ブレファリズマの性成熟過程におけるガモン1遺伝子の発現

    杉浦真由美; 川原聖子; 春本晃江

    日本原生動物学会第37回大会, 2004年11月, false

  • 繊毛虫ブレファリズマにおけるガモン1遺伝子のゲノム配列とその上流配列の決定

    高見梨沙; 杉浦真由美; 春本晃江

    日本原生動物学会第37回大会, 2004年11月, false

  • 繊毛虫ブレファリズマのII型細胞で接合誘導時に発現する遺伝子の単離

    田中悠里; 杉浦真由美; 春本晃江

    日本原生動物学会第37回大会, 2004年11月, false

  • 繊毛虫ブレファリズマの性フェロモンの発現を制御する環境要因と内部要因

    杉浦真由美; 川原聖子; 春本晃江

    日本動物学会第75回大会, 2004年09月, false

  • 繊毛虫ブレファリズマにおけるガモン1遺伝子の上流配列の同定

    高見梨沙; 杉浦真由美; 春本晃江

    日本動物学会第75回大会, 2004年09月, false

  • 繊毛虫ブレファリズマの有性生殖(接合)誘導時に特異的に発現する遺伝子の単離

    田中悠里; 杉浦真由美; 春本晃江

    日本動物学会第75回大会, 2004年09月, false

  • 繊毛虫ブレファリズマの接合誘導物質(ガモン1)の大量発現系の構築

    杉浦真由美; 春本晃江

    日本原生動物学会第36回大会, 2003年11月, false

  • Regulation of specific expression of the conjugation-inducing substance, gamone 1, in the ciliate Blepharisma japonicum

    Mayumi Sugiura; Terue Harumoto

    4 th European Congress of Protistology and 10 th European Conference on Ciliate Biology, 2003年08月, true

  • ブレファリズマにおけるガモン1遺伝子の発現

    杉浦真由美; 春本晃江

    日本原生動物学会第34回大会, 2001年11月, false

  • cDNA sequence determination of blepharmone, a conjugation-inducing glycoprotein of the ciliate Blepharisma japonicum

    Mayumi Sugiura; Terue Harumoto

    XI International Congress of Protozoology-ICOP, 2001年07月, true

  • Complete amino acid sequence of the conjugation-inducing glycoprotein ( blepharmone ) in the ciliate Blepharisma japonicum

    Mayumi Sugiura; Terue Harumoto

    FASEB Summer Research Conferences, Ciliate Molecular Biology, 2001年07月, true

  • ブレファリズマにおける接合誘導物質ガモン1のcDNAクローニング

    杉浦真由美; 春本晃江

    日本動物学会第71回大会, 2000年09月, false

  • ブレファリズマにおける遺伝子単離へ向けてのガモン1の単離精製

    杉浦真由美; 春本晃江

    日本原生動物学会第32回大会, 1999年11月, false

  • ブレファリズマの接合誘導時に見られる形態変化

    吉村千代; 杉浦真由美; 春本晃江

    日本原生動物学会第32回大会, 1999年11月, false

  • ブレファリズマの接合誘導物質ガモン1の糖鎖構造の推定と新しい単離精製法

    杉浦真由美; 春本晃江

    日本動物学会第70回大会, 1999年09月, false

所属学協会

  • 日本原生生物学会

  • 日本動物学会

  • 日本分子生物学会

  • Japan Society of Protistology

  • The Zoological Society of Japan

  • The Molecular Biology Society of Japan



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