鍵和田　聡KAGIWADA Satoshiカギワダ　サトシ

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プロフィール情報

• 姓

  鍵和田

• 名

  聡

学位

• 博士（理学）, 京都大学

研究キーワード

• 生体膜、リン脂質、膜輸送、タンパク質輸送、酵母、小胞体、ゴルジ体、脂質輸送タンパク質、リン脂質代謝、遺伝子発現、

研究分野

• ライフサイエンス, 細胞生物学
• ライフサイエンス, 分子生物学

経歴

• 2016年06月, 奈良女子大学研究院自然科学系・教授
• 2006年04月, 2016年05月, 奈良女子大学研究院自然科学系・准教授
• 1998年04月, 2006年03月, 奈良女子大学理学部・助教授
• 1997年04月, 1998年03月, 科学技術振興事業団研究員
• 1995年05月, 1997年03月, 理化学研究所基礎科学特別研究員
• 1993年04月, 1993年05月, 日本学術振興会特別研究員
• ヒューマンフロンティアサイエンスプログラム長期研究員

学歴

• 1993年, 京都大学, 理学研究科, 生物物理学
• 1988年, 京都大学, 理学部

担当経験のある科目（授業）

• 細胞機構学演習Ⅳ, 奈良女子大学
• 細胞機構学演習Ⅲ, 奈良女子大学
• 細胞機構学演習Ⅱ, 奈良女子大学
• 細胞機構学演習Ⅰ, 奈良女子大学
• 生物環境科学展開実習Ⅲ, 奈良女子大学
• 生物環境科学基礎演習Ⅰ, 奈良女子大学
• 実践生物環境科学演習Ⅱ, 奈良女子大学
• 化学生物環境学入門, 奈良女子大学
• 生物環境科学基礎演習Ⅱ, 奈良女子大学
• 細胞機構学特論, 奈良女子大学
• 細胞機構学セミナーⅢ, 奈良女子大学
• 細胞機構学セミナーⅠ, 奈良女子大学
• 分子・細胞生物学特論３, 奈良女子大学
• 細胞機構学セミナーIII, 奈良女子大学
• 細胞機構学セミナーI, 奈良女子大学
• 細胞機構学セミナーⅣ, 奈良女子大学
• 細胞機構学セミナーⅡ, 奈良女子大学
• 細胞機構学特論Ⅱ, 奈良女子大学
• 生物環境科学基礎実習IIA, 奈良女子大学
• 生物環境科学基礎演習IIA, 奈良女子大学
• 生物環境科学基礎実習IIB, 奈良女子大学
• 生物環境科学基礎演習IIB, 奈良女子大学
• 基礎生物学実習Ⅱ, 奈良女子大学
• 基礎生物学２, 奈良女子大学
• 展開実習Ⅲ, 奈良女子大学
• 基礎細胞生物学, 奈良女子大学
• 生物学実験Ⅱ, 奈良女子大学
• 生物科学演習Ⅰ, 奈良女子大学
• 卒業研究II, 奈良女子大学
• 展開実習III, 奈良女子大学
• 生物科学演習II, 奈良女子大学
• 生物科学特論６, 奈良女子大学
• 生物科学特別研究IV, 奈良女子大学
• 生物科学特別研究II, 奈良女子大学
• 生物科学特別研究III, 奈良女子大学
• 生物科学特別研究I, 奈良女子大学
• 細胞機構学特論２, 奈良女子大学
• 生物学実験II, 奈良女子大学
• 展開実習, 奈良女子大学
• 生物学特別講義２, 奈良女子大学
• 生物科学英語III, 奈良女子大学
• 生物学実験２, 奈良女子大学
• 生物科学特別講義II, 奈良女子大学
• 生物学実験１, 奈良女子大学
• 生物科学２, 奈良女子大学
• 生物学特別講義V, 奈良女子大学
• 細胞生物学実習, 奈良女子大学
• 細胞生物学, 奈良女子大学
• 生体膜構造・機能論演習, 奈良女子大学
• 生体膜構造・機能論, 奈良女子大学
• 細胞機構学セミナーIV, 奈良女子大学
• 細胞機構学セミナーII, 奈良女子大学
• 細胞機構学特論II, 奈良女子大学
• 細胞機構学実習, 奈良女子大学
• 細胞機構学, 奈良女子大学
• 生物科学英語I, 奈良女子大学
• 基礎生物学実験II, 奈良女子大学
• 生化学, 奈良女子大学

所属学協会

• 日本生化学会
• 日本分子生物学会
• American Society for Microbiology
• 酵母研究会

Ⅱ．研究活動実績

論文

• 英語, Journal of Fungi, The Fission Yeast RNA-Binding Protein Meu5 Is Involved in Outer Forespore Membrane Breakdown during Spore Formation, 2020年11月, 6, 4, 280, https://doi.org/10.3390/jof6040284
• 査読あり, 英語, JOURNAL OF BIOCHEMISTRY, Induction of intranuclear membranes by overproduction of Opi1p and Scs2p, regulators for yeast phospholipid biosynthesis, suggests a mechanism for Opi1p nuclear translocation, Miki Masuda; Ayaka Oshima; Tetsuko Noguchi; Satoshi Kagiwada, In the yeast Saccharomyces cerevisiae, the expression of phospholipid biosynthetic genes is suppressed by the Opi1p negative regulator. Opi1p enters into the nucleoplasm from the nuclear membrane to suppress the gene expression under repressing conditions. The binding of Opi1p to the nuclear membrane requires an integral membrane protein, Scs2p and phosphatidic acid (PA). Although it is demonstrated that the association of Opi1p with membranes is affected by PA levels, how Opi1p dissociates from Scs2p is unknown. Here, we found that fluorescently labelled Opi1p accumulated on a perinuclear region in an Scs2p-dependent manner. Electron microscopic analyses indicated that the perinuclear region consists of intranuclear membranes, which may be formed by the invagination of the nuclear membrane due to the accumulation of Opi1p and Scs2p in a restricted area. As expected, localization of Opi1p and Scs2p in the intranuclear membranes was detected by immunoelectron microscopy. Biochemical analysis showed that Opi1p recovered in the membrane fraction was detergent insoluble while Scs2p was soluble, implying that Opi1p behaves differently from Scs2p in the fraction. We hypothesize that Opi1p dissociates from Scs2p after targeting to the nuclear membrane, making it possible to be released from the membrane quickly when PA levels decrease., 2016年03月, 159, 3, 351, 361, 研究論文（学術雑誌）, 10.1093/jb/mvv105

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• 査読あり, 英語, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Coordinated regulation by two VPS9 domain-containing guanine nucleotide exchange factors in small GTPase Rab5 signaling pathways in fission yeast, Yuta Tsukamoto; Satoshi Kagiwada; Sayuri Shimazu; Kaoru Takegawa; Tetsuko Noguchi; Masaaki Miyamoto, The small GTPase Rab5 is reported to regulate various cellular functions, such as vesicular transport and endocytosis. VPS9 domain-containing proteins are thought to activate Rab5(s) by their guaninenucleotide exchange activities. Numerous VPS9 proteins have been identified and are structurally conserved from yeast to mammalian cells. However, the functional relationships among VPS9 proteins in cells remain unclear. Only one Rab5 and two VPS9 proteins were identified in the Schizosaccharomyces pombe genome. Here, we examined the cellular function of two VPS9 proteins and the relationship between these proteins in cellular functions. Vps901-GFP and Vps902-GFP exhibited dotted signals in vegetative and differentiated cells. vps901 deletion mutant (Delta vps901) cells exhibited a phenotype deficient in the mating process and responses to high concentrations of ions, such as calcium and metals, and Delta vps901 Delta vps902 double mutant cells exhibited round cell shapes similar to ypt5-909 (Rab5 mutant allele) cells. Deletion of both vps901 and vps902 genes completely abolished the mating process and responses to various stresses. A lack of vacuole formation and aberrant inner cell membrane structures were also observed in Delta vps901 Delta vps902 cells by electron microscopy. These data strongly suggest that Vps901 and Vps902 are cooperatively involved in the regulation of cellular functions, such as cell morphology, sexual development, response to ion stresses, and vacuole formation, via Rab5 signaling pathways in fission yeast cells. (C) 2015 Elsevier Inc. All rights reserved., 2015年03月, 458, 4, 802, 809, 研究論文（学術雑誌）, 10.1016/j.bbrc.2015.02.031

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• 査読あり, 英語, Algal Research, Colony sheath formation is accompanied by shell formation and release in the green alga Botryococcus braunii (race B), 鍵和田聡, 2015年02月, 8, 214-223
• 査読あり, 英語, Experimental Eye Research, Immediate differentiation of neuronal cells from stem/progenitor-like cells in the avian iris tissues, Tamami Matsushita; Ai Fujihara; Lars Royall; Satoshi Kagiwada; Mitsuko Kosaka; Masasuke Araki, A simple culture method that was recently developed in our laboratory was applied to the chick iris tissues to characterize neural stem/progenitor-like cells. Iris tissue is a non-neuronal tissue and does not contain any neuronal cells. In the present study we isolated iris tissues from chick embryos just prior to hatching. The isolated iris pigmented epithelium (IPE) or the stroma was embedded in Matrigel and cultured in Dulbecco's MEM supplemented with either fetal bovine serum or the synthetic serum replacement solution B27. Within 24h of culture, elongated cells with long processes extended out from the explants of both tissues and were positively stained for various neuronal markers such as transitin, Tuj-1 and acetylated tubulin. After a longer culture period, cells positive for photoreceptor markers like rhodopsin, iodopsin and visinin were found, suggesting that the iris tissues contain retinal stem/progenitor-like cells. Several growth factors were examined to determine their effects on neuronal differentiation. EGF was shown to dramatically enhance neuronal cell differentiation, particularly the elongation of neuronal fibers. The addition of exogenous FGF2, however, did not show any positive effects on neuronal differentiation, although FGF signaling inhibitor, SU5402, suppressed neuronal differentiation. The results show that neuronal stem/progenitor-like cells can differentiate into neuronal cells immediately after they are transferred into an appropriate environment. This process did not require any exogenous factors, suggesting that neural stem/progenitor-like cells are simply suppressed from neuronal differentiation within the tissue, and isolation from the tissue releases the cells from the suppression mechanism. © 2014 Elsevier Ltd., 2014年, 123, 16, 26, 研究論文（学術雑誌）, 10.1016/j.exer.2014.04.007

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• 査読あり, 英語, JOURNAL OF CELL SCIENCE, Osh proteins regulate COPII-mediated vesicular transport of ceramide from the endoplasmic reticulum in budding yeast, Kentaro Kajiwara; Atsuko Ikeda; Auxiliadora Aguilera-Romero; Guillaume A. Castillon; Satoshi Kagiwada; Kentaro Hanada; Howard Riezman; Manuel Muniz; Kouichi Funato, Lipids synthesized at the endoplasmic reticulum (ER) are delivered to the Golgi by vesicular and non-vesicular pathways. ER-to-Golgi transport is crucial for maintaining the different membrane lipid composition and identities of organelles. Despite their importance, mechanisms regulating transport remain elusive. Here we report that in yeast coat protein complex II (COPII) vesicle-mediated transport of ceramide from the ER to the Golgi requires oxysterol-binding protein homologs, Osh proteins, which have been implicated in lipid homeostasis. Because Osh proteins are not required to transport proteins to the Golgi, these results indicate a specific requirement for the Osh proteins in the transport of ceramide. In addition, we provide evidence that Osh proteins play a negative role in COPII vesicle biogenesis. Together, our data suggest that ceramide transport and sphingolipid levels between the ER and Golgi are maintained by two distinct functions of Osh proteins, which negatively regulate COPII vesicle formation and positively control a later stage, presumably fusion of ceramide-enriched vesicles with Golgi compartments., 2014年01月, 127, 2, 376, 387, 研究論文（学術雑誌）, 10.1242/jcs.132001

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• 査読あり, 英語, PLoS ONE, Transformation of lipid bodies related to hydrocarbon accumulation in a green alga, Botryococcus braunii (race B), Reiko Suzuki; Naoko Ito; Yuki Uno; Ichiro Nishii; Satoshi Kagiwada; Sigeru Okada; Tetsuko Noguchi, The colonial microalga Botryococcus braunii accumulates large quantities of hydrocarbons mainly in the extracellular space most other oleaginous microalgae store lipids in the cytoplasm. Botryococcus braunii is classified into three principal races (A, B, and L) based on the types of hydrocarbons. Race B has attracted the most attention as an alternative to petroleum by its higher hydrocarbon contents than the other races and its hydrocarbon components, botryococcenes and methylsqualenes, both can be readily converted into biofuels. We studied race B using fluorescence and electron microscopy, and clarify the stage when extracellular hydrocarbon accumulation occurs during the cell cycle, in a correlation with the behavior and structural changes of the lipid bodies and discussed development of the algal colony. New accumulation of lipids on the cell surface occurred after cell division in the basolateral region of daughter cells. While lipid bodies were observed throughout the cell cycle, their size and inclusions were dynamically changing. When cells began dividing, the lipid bodies increased in size and inclusions until the extracellular accumulation of lipids started. Most of the lipids disappeared from the cytoplasm concomitant with the extracellular accumulation, and then reformed. We therefore hypothesize that lipid bodies produced during the growth of B. braunii are related to lipid secretion. New lipids secreted at the cell surface formed layers of oil droplets, to a maximum depth of six layers, and fused to form flattened, continuous sheets. The sheets that combined a pair of daughter cells remained during successive cellular divisions and the colony increased in size with increasing number of cells. Copyright © 2013 Suzuki et al., 2013年12月05日, 8, 12, e81626, 研究論文（学術雑誌）, 10.1371/journal.pone.0081626

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• 査読あり, 英語, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Human VAPA and the yeast VAP Scs2p with an altered proline distribution can phenocopy amyotrophic lateral sclerosis-associated VAPB(P56S), Shoko Nakamichi; Kumiko Yamanaka; Mai Suzuki; Toshio Watanabe; Satoshi Kagiwada, A human isoform of the vesicle-associated membrane protein-associated proteins (VAPs), VAPB, causes amyotrophic lateral sclerosis eight due to the missense mutation of Pro-56, whereas human VAPA and the yeast VAR Scs2p proteins are not significantly affected by similar mutations. We have found that VAPA and Scs2p have three prolines present in a conserved region however VAPB has only two prolines in this region. Consequently, this mutation in VAPB (VAPB(P56S)) leaves a single proline in this region whereas other VAPs can retain two proline residues even if the proline equivalent to the Pro-56 is substituted. When Scs2p and VAPA were mutated to be equivalent to VAPB(P56S) in terms of the distribution of proline residues in this region, Scs2p became inactive and aggregated, and VAPA localize to membranous aggregates indistinguishable from those induced by VAPB(P56S). This suggests that the appropriate distribution of three conserved prolines, not the existence of a particular proline, confers VAPA and Scs2p resistance to the Pro-56 mutation and, therefore, is critical for VAR activities. (C) 2010 Elsevier Inc. All rights reserved., 2011年01月, 404, 2, 605, 609, 研究論文（学術雑誌）, 10.1016/j.bbrc.2010.12.011

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• 査読あり, 英語, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, The yeast VAP homolog Scs2p has that is correlated a phosphoinositide-binding ability with its activity, Satoshi Kagiwada; Misa Hashimoto, The yeast VAMP-associated protein (VAP) homolog Scs2p is an endoplasmic reticulum (ER)/nuclear membrane protein that binds to an FFAT (diphenylalanine in an acidic tract) motif found in various lipid-metabolic proteins, including Opi1p, a negative regulator of phospholipid biosynthesis. Here, we show that Scs2p is a novel phosphoinositide-binding protein that can bind to phosphatidylinositol, monophosphates and bisphosphates in vitro. The phosphoinositide-binding domain was assigned to the N-terminal major sperm protein (MSP) domain which also contains the FFAT-binding domain. When several lysine residues in the MSP domain were substituted for alanine, the resulting mutant Scs2 proteins lost the phosphoinositide-binding ability and failed to complement the inositol auxotrophy of an scs2 deletion strain. However, the mutant proteins still localized in the ER/nuclear membrane, in a similar manner to wild-type Scs2p. These results suggest the possibility that Scs2p activity is regulated by phosphoinositides to coordinate phospholipid biosynthesis in response to changes in phospholipid composition. (C) 2007 Elsevier Inc. All rights reserved., 2007年12月, 364, 4, 870, 876, 研究論文（学術雑誌）, 10.1016/j.bbrc.2007.10.079

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• 査読あり, 英語, Marine biotechnology, Docosahexaenoic acid production and lipid-body formation in Schizochytrium limacinum SR21., 鍵和田聡, 2006年05月, 8, 3, 319-327, 327
• 査読あり, 英語, PLANT SCIENCE, Characterization of coat proteins of COPI- and clathrin-coated vesicles in the unicellular green alga Botryococcus braunii, S Kagiwada; S Sugita; Y Masaike; S Kato; T Noguchi, Unicellular green algae are a useful system for studying intracellular protein transport in plant cells because they possess various plantspecific features such as cell wall formation and isolated Golgi bodies with distinct multilayered cisternae. In this study, two genes encoding the P-COP subunit of COPI-coated vesicles and the sigma 1 subunit of the AP-1 complex of clathrin-coated vesicles were cloned from the green alga Botryococcus braunii. The beta-COP gene contains an open reading frame (ORF) that codes for a 947-amino acid protein with a deduced molecular mass of 104 kDa, while the sigma 1 gene contains an ORF for a 159-amino acid protein with a deduced molecular mass of 19 kDa. Polyclonal antibodies raised against these proteins recognized proteins with predicted molecular masses in cell lysates prepared from B. braunii and the green alga Chlamydomonas reinhardtii. Cell fractionation studies revealed that both beta-COP and sigma 1 were associated with membranous compartments. By immunoelectron microscopy, beta-COP was found mainly on Golgi cisternae and to a lesser extent on Golgi-associated vesicles at all levels of the Golgi bodies, consistent with a proposed role of COPI-coated vesicles in inter-compartmental transport between Golgi cisternae. (c) 2005 Elsevier Ireland Ltd. All rights reserved., 2005年10月, 169, 4, 668, 679, 研究論文（学術雑誌）, 10.1016/j.plantsci.2005.05.027

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• 査読あり, 英語, PLANT SCIENCE, Cytoskeleton-dependent polarized secretion of arylsulfatase in the unicellular green alga, Chlamydomonas reinhardtii, S Kagiwada; Nakamae, I; M Kayukawa; S Kato, In the green alga Chlamydomonas reinhardtii, protein secretion has not been well investigated at the molecular level although the process plays important roles in cell wall biogenesis, the mating process, and stress response. Here, we studied the cytoskeleton dependence of Chlamydomonas protein secretion by examining the effects of cytoskeleton-disrupting reagents. Protein transport activity was monitored by the secretion of arylsulfatase (ARS), a glycoprotein which is synthesized in response to sulfur deprivation and transported to the periplasmic space. Microtubule-disrupting reagents, oryzalin and nocodazole, inhibited ARS secretion in a dose-dependent manner, while actin-disrupting reagents, cytochalasins B and D, induced up to a 1.4-fold increase in ARS secretion. The effect of oryzatin on the intracellular ARS distribution was different from that of brefeldin A (BFA), which disrupts the ER to Golgi protein transport. Immunofluorescence analysis using an anti-ARS polyclonal antibody revealed specific punctate staining on the anterior side of the cell surface of cells cultured in sulfur-depleted medium. This staining was not observed when the cells were treated with oryzalin. These results suggest that Chlamydomonas protein secretion is dependent on microtubules and inhibited by actin-related cytoskeletons. This cytoskeleton dependence is similar to that found in mammalian cells rather than that found in plant cells. (C) 2004 Elsevier Ireland Ltd. All rights reserved., 2004年06月, 166, 6, 1515, 1524, 研究論文（学術雑誌）, 10.1016/j.plantsci.2004.02.003

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• 査読あり, 英語, JOURNAL OF BIOCHEMISTRY, Role of the yeast VAP homolog, Scs2p, in INO1 expression and phospholipid metabolism, S Kagiwada; R Zen, In the yeast Saccharomyces cerevisiae, the expression of phospholipid biosynthetic genes, including the INO1 gene (encoding inositol-1-phosphate synthase), is coordinately regulated by a cis-acting transcriptional element, UAS(INO) (inositol-sensitive upstream activating sequence). For this paper we studied the effect of SCS2 disruption on INO1 expression. SCS2 encodes a type II membrane protein and its deletion leads to inositol auxotrophy at temperatures above 34degreesC. We found that the expression of the INO1 gene was reduced in the scs2Delta strain even when the cells were cultured under derepressing conditions for INO1 expression. However, the beta-galactosidase gene fused with the INO1 promoter region was expressed normally in the scs2Delta strain. The phospholipid composition of scs2Delta cells was not dramatically changed compared with wild-type cells at 28degreesC, but the phosphatidylinositol level was reduced in scs2Delta cells cultured at 34degreesC. In addition, elevated phosphatidylcholine synthesis through the CDP-choline pathway was observed in the scs2Delta strain, and the disruption of genes involved in the CDP-choline pathway rescued the INO1 expression defect of the scs2Delta strain. These results indicate that Scs2p can contribute to coordinated phospholipid metabolism including INO1 expression by regulating phosphatidylcholine synthesis through the CDP-choline pathway., 2003年04月, 133, 4, 515, 522, 研究論文（学術雑誌）, 10.1093/jb/mvg068

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• 査読あり, 英語, JOURNAL OF BIOCHEMISTRY, Effect of brefeldin A on melatonin secretion of chick pineal cells, T Hirota; S Kagiwada; T Kasahara; T Okano; M Murata; Y Fukada, Melatonin is secreted from the pineal gland in a circadian manner. It is well established that the synthesis of melatonin shows a diurnal rhythm reflecting a daily change in serotonin N-acetyltransferase (NAT) activity, and the overall secretion of melatonin requires a cellular release process, which is poorly understood. To investigate the possible involvement of Golgi-derived vesicles in the release, we examined the effect of brefeldin A (BFA), a reversible inhibitor of Golgi-mediated secretion, on melatonin secretion of cultured chick pineal cells. We show here that treatment with BFA completely disassembles the Golgi apparatus and reduces melatonin secretion. In more detailed time course experiments, however, the inhibition of melatonin secretion is only observed after the removal of BFA in parallel with the reassembly of the Golgi apparatus. This inhibition of melatonin secretion is not accompanied by accumulation of melatonin in the cells. These observations indicate that chick pineal melatonin is released independently of the Golgi-derived vesicles, and suggest inhibition of melatonin synthesis after the removal of BFA. By measuring the activities and mRNA levels of melatonin-synthesizing enzymes, we found that the removal of BFA specifically inhibits NAT activity at the protein level. On the other hand, BFA causes no detectable phase-shift of the chick pineal oscillator regulating the circadian rhythm of melatonin secretion. The results presented here suggest that the Golgi-mediated vesicular transport is involved in neither the melatonin release nor the time-keeping mechanism of the circadian oscillator, but rather contributes to the regulation of NAT activity., 2001年01月, 129, 1, 51, 59, 研究論文（学術雑誌）
• 査読あり, 英語, MOLECULAR BIOLOGY OF THE CELL, Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: Relationship to "bypass sec14p" and inositol auxotrophy, MP Rivas; BG Kearns; ZG Xie; SL Guo; MC Sekar; K Hosaka; S Kagiwada; JD York; VA Bankaitis, SacIp dysfunction results in bypass of the requirement for phosphatidylinositol transfer protein (Sec14p) function in yeast Golgi processes. This effect is accompanied by alterations in inositol phospholipid metabolism and inositol auxotrophy. Elucidation of how sac1 mutants effect "bypass Sec14p" will provide insights into Sec14p function in vivo. We now report that, in addition to a dramatic accumulation of phosphatidylinositol-4-phosphate, sad mutants also exhibit a specific acceleration of phosphatidylcholine biosynthesis via the CDP-choline pathway. This phosphatidylcholine metabolic phenotype is sensitive to the two physiological challenges that abolish bypass Sec14p in sac1 strains; i.e. phospholipase D inactivation and expression of bacterial diacylglycerol (DAG) kinase. Moreover, we demonstrate that accumulation of phosphatidylinositol-4-phosphate in sad mutants is insufficient to effect bypass Sec14p. These data support a model in which phospholipase D activity contributes to generation of DAG that, in turn, effects bypass Sec14p. A significant fate for this DAG is consumption by the CDP-choline pathway. Finally, we determine that CDP-choline pathway activity contributes to the inositol auxotrophy of sac1 strains in a novel manner that does not involve obvious defects in transcriptional expression of the INO1 gene., 1999年07月, 10, 7, 2235, 2250, 研究論文（学術雑誌）
• 査読あり, 英語, JOURNAL OF BACTERIOLOGY, The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism, S Kagiwada; K Hosaka; M Murata; J Nikawa; A Takatsuki, The Saccharomyces cerevisiae SCS2 gene has been cloned as a suppressor of inositol auxotrophy of CSE1 and hac1/ire15 mutants (J. Nikawa, A. Murakami, E. Esumi, and K. Hosaka, J. Biochem. 118:39-45, 1995) and has homology with a synaptobrevin/VAMP-associated protein, VAP-33, cloned from Aplysia californica (P. A. Skehel, K. C. Martin, E. R. Kandel, and D. Bartsch, Science 269:1580-1583, 1995). In this study we have characterized an SCS2 gene product (Scs2p). The product has a molecular mass of 35 kDa and is C-terminally anchored to the endoplasmic reticulum, with the bulk of the protein located in the cytosol. The disruption of the SCS2 gene causes yeast cells to exhibit inositol auxotrophy at temperatures of above 34 degrees C, Genetic studies reveal that the overexpression of the INO1 gene rescues the inositol auxotrophy of the SCS2 disruption strain. The significant primary structural feature of Scs2p is that the protein contains the 16-amino-acid sequence conserved in yeast and mammalian cells. The sequence is required for normal Scs2p function, because a mutant Scs2p that lacks the sequence does not complement the inositol auxotrophy of the SCS2 disruption strain. Therefore, the Scs2p function might be conserved among eukaryotic cells., 1998年04月, 180, 7, 1700, 1708, 研究論文（学術雑誌）
• 査読あり, 英語, NATURE, Essential role for diacylglycerol in protein transport from the yeast Golgi complex, BG Kearns; TP McGee; P Mayinger; A Gedvilaite; SE Phillips; S Kagiwada; VA Bankaitis, Yeast phosphatidylinositol transfer protein (Sec14p) is required for the production of secretory vesicles from the Golgi. This requirement can be relieved by inactivation of the cytosine 5'-diphosphate (CDP)-choline pathway for phosphatidylcholine biosynthesis, indicating that Sec14p is an essential component of a regulatory pathway linking phospholipid metabolism with vesicle trafficking (the Sec14p pathway(1-6)). Sac1p (refs 7 and 8) is an integral membrane protein related to inositol-5-phosphatases such as synaptojanin(9), a protein found in rat brain, Here we show that defects in Sac1p also relieve the requirement for Sec14p by altering phospholipid metabolism so as to expand the pool of diacylglycerol (DAG) in the Golgi. Moreover, although short-chain DAG improves secretory function in strains with a temperature-sensitive Sec14p, expression of diacylglycerol kinase from Escherichia call further impairs it. The essential function of Sec14p may therefore be to maintain a sufficient pool of DAG in the Golgi to support the production of secretory vesicles., 1997年05月, 387, 6628, 101, 105, 研究論文（学術雑誌）
• 査読あり, 英語, EMBO JOURNAL, Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis, M Fang; BG Kearns; A Gedvilaite; S Kagiwada; M Kearns; MKY Fung; VA Bankaitis, The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation of the CDP-choline pathway for phosphatidylcholine biosynthesis. These findings indicate that Sec14p functions to alleviate a CDP-choline pathway-mediated toxicity to yeast Golgi secretory function, We now report that this toxicity is manifested through the action of yeast Kes1p, a polypeptide that shares homology with the ligand-binding domain of human oxysterol binding protein (OSBP), Identification of Kes1p as a negative effector for Golgi function provides the first direct insight into the biological role of any member of the OSBP family, and describes a novel pathway for the regulation of Golgi-derived transport vesicle biogenesis., 1996年12月, 15, 23, 6447, 6459, 研究論文（学術雑誌）
• 査読あり, 英語, GENETICS, The yeast BSD2-1 mutation influences both the requirement for phosphatidylinositol transfer protein function and derepression of phospholipid biosynthetic gene expression in yeast, S Kagiwada; BG Kearns; TP McGee; M Fang; K Hosaka; VA Bankaitis, The BSD2-1 allele renders Saccharomyces cerevisiae independent of its normally essential requirement for phosphatidylinositol transfer protein (Sec14p) in the stimulation of Golgi secretory function and cell viability. We now report that BSD2-1 yeast mutants also exhibit yet another phenotype, an inositol auxotrophy. We demonstrate that the basis for this Ino(-) phenotype is the inability of BSD2-1 strains to derepress transcription of lNO1, the structural gene for the enzyme that catalyzes the committed step in de novo inositol biosynthesis in yeast. This constitutive repression of INO1 expression is mediated through specific inactivation of Ino2p, a factor required for trans-activation of INO1 transcription, and we show that these transcriptional regulatory defects can be uncoupled from the ''bypass Sec14p'' phenotype of BSD2-1 strains. Finally, we present evidence that newly synthesized phosphatidylinositol is subject to accelerated turnover in BSD2-1 mutants and that prevention of this accelerated phosphatidylinositol turnover in turn negates suppression of Sec14p defects by BSD2-1. We propose that, in BSD2-1 strains, a product(s) generated by phosphatidylinositol turnover coordinately modulates the activities of both the Sec14p/Golgi pathway and the pathway through which transcription of phospholipid biosynthetic genes is derepressed., 1996年06月, 143, 2, 685, 697, 研究論文（学術雑誌）
• 査読あり, 英語, BIOCHIMICA ET BIOPHYSICA ACTA, FUSION OF DIOLEOYLPHOSPHATIDYLCHOLINE VESICLES INDUCED BY AN AMPHIPHILIC CATIONIC PEPTIDE AND OLIGOPHOSPHATES AT NEUTRAL PH, M MURATA; Y SHIRAI; R ISHIGURO; S KAGIWADA; Y TAHARA; S OHNISHI; S TAKAHASHI, Peptide E5 is an analogue of the fusion peptide of influenza virus hemagglutinin and K5 is a cationic peptide which has an arrangement of electric charges complementary to that of E5. We reported that a stoichiometric mixture of E5 and K5 caused fusion of large unilamellar vesicles (LUV) of neutral phospholipids (Murata, M., Kagiwada, S., Takahashi, S. and Ohnishi, S. (1991) J. Biol. Chem. 266, 14353-14358). K5 caused fusion of LUV composed of dioleoylphosphatidylcholine (DOPC) at pH > 10, but not at neutral pH. In the presence of oligophosphates, such as 1 mM ATP, GTP, or polyphosphate, K5 caused rapid and efficient fusion of DOPC LUV at neutral pH without hydrolysis of oligophosphate groups, but another anions such as citrate, acetate, AMP, phosphate, or EDTA were ineffective. The peptide/oligophosphate-induced fusion behaviors have been investigated by a fluorescence resonance energy transfer assay for lipid mixing of LUV and negative staining electron microscopy. At higher ionic strengths (> 0.3 M KCl) or in the presence of 5.0 mM MgCl2, the fusion was inhibited. Even at the inhibitory conditions, the association of K5 with lipid vesicles at neutral pH was directly confirmed by the Ficoll gradient assay method and by blue shifts of the tryptophan fluorescence of the peptide. A nonhydrolyzable GTP analogue, GTPgammaS, also induced fusion. These observations suggested that the electrostatic interactions between the positive and negative charges of K5 and oligophosphate, respectively, induced complex formation, triggering membrane fusion., 1993年10月, 1152, 1, 99, 108, 研究論文（学術雑誌）
• 査読あり, 英語, BIOPHYSICAL JOURNAL, SPECIFICITY OF AMPHIPHILIC ANIONIC PEPTIDES FOR FUSION OF PHOSPHOLIPID-VESICLES, M MURATA; S TAKAHASHI; Y SHIRAI; S KAGIWADA; R HISHIDA; S OHNISHI, We have synthesized five amphiphilic anionic peptides derived from E5 peptide [Murata, M., Takahashi, S., Kagiwada, S., Suzuki, A., Ohnishi, S. 1992. Biochemistry 31:1986-1992. E5NN and E5CC are duplications of the N-terminal and the C-terminal halves of E5, respectively, and E5CN is an inversion of the N- and the C-terminal halves. E5P contains a Pro residue in the center of E5 and E8 has 8 Glu residues and 9 Leu residues, We studied fusion of dioleoylphosphatidylcholine (DOPC) large unilamellar vesicles assayed by fluorescent probes. The peptides formed alpha-helical structure with different degrees; E5NN, E5CN, and E8 with high helical content and E5CC and E5P with low helical content. These peptides bound to DOPC vesicles at acidic pH in proportion to the helical content of peptide. The peptides caused leakage of DOPC vesicles which increased with decreasing pH. The leakage was also proportional to the helicity of peptide. Highly helical peptides E5NN, E5CN, and E8 caused hemolysis at acidic pH but not at neutral pH. The fusion activity was also dependent on the helicity of peptides. In fusion induced by an equimolar mixture of E5 analogues and K5 at neutral pH, E8, E5NN, and E5CN were most active but E5CC did not cause fusion. In fusion induced by E5-analogue peptides alone, E5CN was active at acidic pH but not at neutral pH. Other peptides did not cause fusion. Amphiphilic peptides also appear to require other factors to cause fusion., 1993年03月, 64, 3, 724, 734, 研究論文（学術雑誌）
• 査読あり, 英語, JOURNAL OF BIOLOGICAL CHEMISTRY, INVITRO FUSION OF RABBIT LIVER GOLGI MEMBRANES WITH LIPOSOMES, S KAGIWADA; M MURATA; R HISHIDA; M TAGAYA; S YAMASHINA; S OHNISHI, Fusion of Golgi membranes isolated from rabbit liver with liposomes was studied by lipid mixing of fluorescent lipid analogues and internal content mixing and by electron microscopic observation of transfer of horseradish peroxidase from liposomes into Golgi membranes. A monoclonal antibody was used to confirm fusion of Golgi membranes but not other contaminating vesicles. Fusion was rapid and efficient, reaching about 20% of the maximum after a 5-min incubation using small or large unilamellar dioleoylphosphatidylcholine vesicles. The fusion was dependent on temperature, decreasing at lower temperatures, and becoming nearly zero below 10-degrees-C. The addition of ATP, GTP, cytosolic factors, or N-ethylmaleimide did not affect fusion. Treatments of Golgi membranes with 0.1 M Na2CO3 or 1 M KCl did not cause any changes in fusion. However, treatment with proteases inhibited fusion. These results suggest that Golgi integral membrane protein(s) are involved in fusion. Changing the medium to an isoosmotic substance, sucrose, in place of KCl or NaCl inhibited fusion. The binding assay of fluorescent liposomes to Golgi membranes showed that lowering the temperature or replacing salts with sucrose did not affect binding. However, treatment of Golgi membranes with proteases inhibited binding. Addition of phosphatidylserine or phosphatidylethanolamine to dioleoylphosphatidylcholine liposomes caused a 2-fold increase in binding and fusion. Fusion between Golgi membranes by themselves did not occur. These results provide some information on the mechanism of intracellular vesicular transport., 1993年01月, 268, 2, 1430, 1435, 研究論文（学術雑誌）
• 査読あり, 英語, BIOLOGY OF THE CELL, INTERACTION OF THE GOLGI MEMBRANES ISOLATED FROM RABBIT LIVER WITH MICROTUBULES INVITRO, M MURATA; TJ ITOH; S KAGIWADA; R HISHIDA; H HOTANI; S OHNISHI, We have developed a reconstituted model system to study the interaction of the Golgi membranes isolated from rabbit liver with taxol-stabilized bovine-brain microtubules without microtubule-associated proteins (MAPs). The Golgi membranes are associated with microtubules. The sheets of vesicles and the membranous tubules are observed along microtubules by direct visualization using differential-interference-contrast, dark field, or fluorescence microscopy. The monoclonal antibody against Golgi membranes suggests that the Golgi membranes, but not the contaminating vesicles, are interacting with microtubules. The degree of association is assayed quantitatively using rhodamine-labeled microtubules after separation of the complex from unbound microtubules by centrifugation upon sucrose gradient, The association is inhibited by crude MAPs, purified MAP2, or 1.0 mM ATP. However, the association neither requires the cytosol from rat liver or bovine brain nor N-ethylmaleimide, brefeldin A, or GTP-gamma-S. The association is mediated by trypsin-sensitive peripheral protein(s) on the Golgi membranes., 1992年, 75, 2, 127, 134, 研究論文（学術雑誌）
• 査読あり, 英語, BIOCHEMISTRY, PH-DEPENDENT MEMBRANE-FUSION AND VESICULATION OF PHOSPHOLIPID LARGE UNILAMELLAR VESICLES INDUCED BY AMPHIPHILIC ANIONIC AND CATIONIC PEPTIDES, M MURATA; S TAKAHASHI; S KAGIWADA; A SUZUKI; S OHNISHI, We studied fusion induced by a 20-amino acid peptide derived from the amino-terminal segment of hemagglutinin of influenza virus A/PR/8/34 [Murata, M., Sugahara, Y., Takahashi, S., & Ohnishi, S. (1987) J. Biochem. (Tokyo) 102, 957-962]. To extend the study, we have prepared several water-soluble amphiphilic peptides derived from the HA peptide; the anionic peptides D4, E5, and E5L contain four and five acidic residues and the cationic peptide K5 has five Lys residues in place of the five Glu residues in E5. Fusion of egg phosphatidylcholine large unilamellar vesicles induced by these peptides is assayed by two different fluorescence methods, lipid mixing and internal content mixing. Fusion is rapid in the initial stage (12-15% within 20 s) and remains nearly the same or slightly increasing afterward. The anionic peptides cause fusion at acidic pH lower than 6.0-6.5, and the cationic peptide causes fusion at alkaline pH higher than 9.0. Leakage and vesiculation of vesicles are also measured. These peptides are bound and associated with vesicles as shown by Ficoll discontinuous gradients and by the blue shift of tryptophan fluorescence. They take an alpha-helical structure in the presence of vesicles. They become more hydrophobic in the pH regions for fusion. When the suspension is made acidic or alkaline, the vesicles aggregate, as shown by the increase in light scattering. The fusion mechanism suggests that the amphiphilic peptides become more hydrophobic by neutralization due to protonation of the carboxyl groups or deprotonation of the lysyl amino groups, aggregate the vesicles together, and interact strongly with lipid bilayers to cause fusion. At higher peptide concentrations, E5 and E5L cause fusion transiently at acidic pH followed by vesiculation., 1992年02月, 31, 7, 1986, 1992, 研究論文（学術雑誌）
• 査読あり, 英語, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, MODIFICATION OF THE N-TERMINUS OF MEMBRANE FUSION-ACTIVE PEPTIDES BLOCKS THE FUSION ACTIVITY, M MURATA; S KAGIWADA; R HISHIDA; R ISHIGURO; S OHNISHI; S TAKAHASHI, 1991年09月, 179, 2, 1050, 1055, 研究論文（学術雑誌）
• 査読あり, 英語, JOURNAL OF BIOLOGICAL CHEMISTRY, MEMBRANE-FUSION INDUCED BY MUTUAL INTERACTION OF THE 2 CHARGE-REVERSED AMPHIPHILIC PEPTIDES AT NEUTRAL PH, M MURATA; S KAGIWADA; S TAKAHASHI; SI OHNISHI, An anionic amphiphilic peptide and the charge-reversed cationic peptide are synthesized. They contain 20 amino acids with the same sequence except for 5 Glu residues for the anionic versus 5 Lys residues for the cationic peptides. Fusion of egg phosphatidylcholine large unilamellar vesicles is assayed with the fluorescent probes by the lipid mixing and the internal content mixing at neutral pH. The peptide mixture causes a rapid and efficient membrane fusion, in spite of no fusions with each peptide by itself. Each peptide takes nearly random coils with a small amount of helix, but the peptide mixture has an ordered helical structure. The equimolar peptide mixture forms a much more hydrophobic complex than those of different molar ratios of peptides and also that of each peptide itself. The equimolar peptide mixture causes the most efficient fusion. Preincubations of two peptides before addition to vesicles cause the slower rates of fusion. The fusion is greatly reduced at higher ionic strength and nearly zero at 800 mM NaCl and 40 mM sodium phosphate. Each peptide and the peptide mixture show the same alpha-helical structure, interact with vesicles, but do not induce fusion at higher ionic strengths. These results suggest that the two peptides interact mutually through the electrostatic Coulombic interaction between the charged groups. The electrically neutralized hydrophobic complex aggregates the separate vesicles together and interacts with the hydrocarbon region of lipid bilayers to cause fusion., 1991年08月, 266, 22, 14353, 14358, 研究論文（学術雑誌）
• 査読あり, 英語, BIOCHEMISTRY, DEVELOPMENT OF A STREAK-CAMERA-BASED TIME-RESOLVED MICROSCOPE FLUOROMETER AND ITS APPLICATION TO STUDIES OF MEMBRANE-FUSION IN SINGLE CELLS, A KUSUMI; A TSUJI; M MURATA; Y SAKO; AC YOSHIZAWA; S KAGIWADA; T HAYAKAWA; S OHNISHI, A time-resolved microscope fluorimeter based on a synchroscan streak camera and a fast pulsed laser system has been developed to measure the fluorescence lifetime decay under the fluorescence microscope. This system allows one to measure the nanosecond fluorescence lifetimes of fluorophores in a small spot (0.8-6.3-mu-m diameter) in single cultured cells under a fluorescence microscope, while the cells are being viewed under a high-power objective lens. A signal acquisition time between a second and a minute was usually sufficient to obtain fluorescence decay curves with good quality for 10(3)-10(5) fluorophores localized in 1-mu-m2 domain. A signal-to-noise ratio better than 30 was obtained for almost-equal-to 30 000 fluorescein-labeled band 3 molecules in a 2-mu-m2 region in a single human erythrocyte ghost after signal accumulation for 30 s. The measured lifetimes for a variety of fluorescent probes attached to proteins in solution and lipids in liposomes showed a good agreement with those measured in a cuvette under standard conditions by time-correlated single photon counting. With the development of this instrument, microscope fluorimetry has become a practical, straightforward, quantitative technique for investigation of molecular processes in single cells in culture. Time-resolved microscope fluorimetry has been applied to observe fusion of liposomes in vitro and that of endosomes in single cells by monitoring resonance energy transfer. Inspection of individual liposomes and endosomes revealed the extent of fusion for each vesicle. Since the use of time-resolved microscope fluorimetry eliminates the need for subcellular fractionation or the complex correction procedures in steady-state microfluorimetry, it greatly simplifies the assay for endosome fusion in vivo. The results showed that extensive fusion of sequentially formed endosomes takes place all over the cell matrix in cultured cells. This suggests that extensive fusion with incoming endosomes takes place in many endosomal compartments, possibly sorting organelles, or that the early endosomes fuse with the preexisting network of tubular cisternae of the endosomal compartment at many points in the network. It is concluded that time-resolved microscope fluorimetry is a powerful noninvasive technique for studies of in situ biochemistry and biophysics using cells and tissues., 1991年07月, 30, 26, 6517, 6527, 研究論文（学術雑誌）
• その他, Journal of Fungi, The Fission Yeast RNA-Binding Protein Meu5 Is Involved in Outer Forespore Membrane Breakdown during Spore Formation, Bowen Zhang; Erika Teraguchi; Kazuki Imada; Yuhei O. Tahara; Shuko Nakamura; Makoto Miyata; Satoshi Kagiwada; Taro Nakamura, In Schizosaccharomyces pombe, the spore wall confers strong resistance against external stress. During meiosis II, the double-layered intracellular forespore membrane (FSM) forms de novo and encapsulates the nucleus. Eventually, the inner FSM layer becomes the plasma membrane of the spore, while the outer layer breaks down. However, the molecular mechanism and biological significance of this membrane breakdown remain unknown. Here, by genetic investigation of an S. pombe mutant (E22) with normal prespore formation but abnormal spores, we showed that Meu5, an RNA-binding protein known to bind to and stabilize more than 80 transcripts, is involved in this process. We confirmed that the E22 mutant does not produce Meu5 protein, while overexpression of meu5+ in E22 restores the sporulation defect. Furthermore, electron microscopy revealed that the outer membrane of the FSM persisted in meu5∆ spores. Investigation of the target genes of meu5+ showed that a mutant of cyc1+ encoding cytochrome c also showed a severe defect in outer FSM breakdown. Lastly, we determined that outer FSM breakdown occurs coincident with or after formation of the outermost Isp3 layer of the spore wall. Collectively, our data provide novel insights into the molecular mechanism of spore formation., 2020年11月13日, 6, 4, 284, 284, 研究論文（学術雑誌）, 10.3390/jof6040284

  PermalinkDOI URL
• 日本語, 生物物理, 一般社団法人 日本生物物理学会, 1PA057 ブレフェルジンAのパルス投与によるニワトリ松果体細胞のメラトニン合成阻害の分子機構, 広田 毅; 鍵和田 聡; 笠原 和起; 岡野 俊行; 深田 吉孝; 村田 昌之, 1999年, 39, S48, 10.2142/biophys.39.S48_1

  DOI URL
• 査読あり, 英語, TIME-RESOLVED LASER SPECTROSCOPY IN BIOCHEMISTRY II, PTS 1 AND 2, DEVELOPMENT OF TIME-RESOLVED MICROFLUORIMETRY AND ITS APPLICATION TO STUDIES OF CELLULAR MEMBRANES, A KUSUMI; A TSUJI; M MURATA; Y SAKO; S KAGIWADA; T HAYAKAWA; SI OHNISHI, 1990年, 1204, 776, 783, 研究論文（国際会議プロシーディングス）, 10.1117/12.17720

  PermalinkDOI URL

MISC

• 査読無し, 日本語, 蛋白質核酸酵素, 共立出版, 酵母におけるイノシトールリン脂質の機能, 鍵和田聡, 1999年06月, 44, 8, 1194-1199, 1199

  Permalink
• 査読無し, 日本語, 化学と生物, Japan Society for Bioscience, Biotechnology, and Agrochemistry, リン脂質輸送タンパク質の新しい機能, 鍵和田聡, 1996年10月, 34, 7, 433-439, 439, 10.1271/kagakutoseibutsu1962.34.433

  PermalinkDOI URL
• 査読無し, 日本語, 実験医学, 細胞内蛋白質輸送におけるリン脂質輸送タンパク質の新しい機能, 鍵和田聡, 1996年08月, 14, 14, 2058-2064, 2064

  Permalink
• 査読無し, 日本語, 生物物理, 細胞内の膜小胞形成に関与する蛋白質, 鍵和田聡, 1992年10月, 32, 5, 268-272
• 査読無し, 日本語, 細胞, 細胞内小胞輸送を制御する蛋白質, 鍵和田聡, 1991年12月, 23, 12, 475-479
• 査読無し, 日本語, 組織培養, 新しい物理化学的手法によるエンドサイトーシス機構の解析, 鍵和田聡, 1991年07月, 17, 7, 220-224
• 査読無し, 日本語, 生物物理, The Biophysical Society of Japan General Incorporated Association, 膜融合活性ペプチドのマイクロマニュピレーションとその応用, 鍵和田聡, 1989年12月, 29, 6, 316-319, 319, 10.2142/biophys.29.6_316

  PermalinkURLDOI URL
• 査読無し, 日本語, 細胞, 膜小胞化と膜融合, 鍵和田聡, 1989年11月, 21, 11, 416-420
• 査読無し, 英語, PHYCOLOGIA, INT PHYCOLOGICAL SOC, BIOSYNTHESIS AND ACCUMULATION OF HYDROCARBONS AND POLYSACCHARIDES IN A COLONIAL GREEN ALGA, BOTRYOCOCCUS BRAUNII RACE B, R. Suzuki; Y. Uno; I. Nishii; S. Kagiwada; T. Noguchi, 2013年07月, 52, 4, 109, 109, 研究発表ペーパー・要旨（国際会議）
• 査読無し, 日本語, 生化学, (公社)日本生化学会, ブレフェルジンAを用いたニワトリ松果体細胞のメラトニン分泌機構の解析, 広田 毅; 鍵和田 聡; 笠原 和起; 岡野 俊行; 村田 昌之; 深田 吉孝, 2000年08月, 72, 8, 1016, 1016

書籍等出版物

• Phospholipid transfer protein function in the yeast Saccharomyces cerevisiae., Plenum Press, 鍵和田聡, 分担, 1996年08月, 83-91, 英語, 査読無し, その他
• Phospholipid transfer proteins: emerging roles in vesicle trafficking, signal transduction, and metabolic regulation, Springer-Verlag, 鍵和田聡, 1996年, 英語, 査読無し, その他

講演・口頭発表等

• 水谷泉美; 岡田茂; 鍵和田聡, 日本農芸化学会2019年度大会, 出芽酵母Saccharomyces cerevisiae内で大量生産させたトリテルペンの細胞壁分画への溶出, 2019年03月, 日本語, 東京, 国内会議
• 中村珠子; 鍵和田聡, 日本農芸化学会2019年度大会, 出芽酵母Saccharomyces cerevisiaeのOpi1タンパク質による膜陥入が生じる核膜領域の形態的解析, 2019年03月, 日本語, 東京, 国内会議
• 鍵和田聡, 平成29年度日本水産学会春季大会, Functional characterization of a unique squalene epoxidase from the freshwater alga Botryococcus braunii, race B, 2018年03月, 英語, 東京, 国内会議
• 池田美咲; 三井菜々香; 竹田綾女; 野口哲子; 岡田茂; 鍵和田聡, 日本農芸化学会2017年度大会, 緑藻Botryococcus brauniiのトリテルペン炭化水素合成酵素の出芽酵母Saccharomyces cerevisiaeを用いた機能解析, 2017年03月, 日本語, 京都女子大学, 国内会議
• 鍵和田聡, 日本水産学会春季大会, cDNA cloning, yeast expression, and functional characterization of 10,11-squalene epoxidase from the freshwater alga Botryococcus braunii, race B, 2017年03月, 英語, 東京海洋大学品川キャンパス, 国内会議
• 桒原 雅子; 児林 美萌; 鍵和田 聡, 第38回日本分子生物学会 第88回日本生化学会大会 共同年会, 出芽酵母Saccharomyces cerevisiaeにおけるリン脂質生合成遺伝子発\n現の負の調節因子Opi1pは正の調節因子Ino2pを分解することで遺伝子発現を抑制\nする, 2015年12月, 日本語, 神戸市, 国内会議
• 内田 英伸; Tsou Chungyau; 福永 有佑; Victor M.E. Ferriols; 鍵和田 聡; 松永 茂樹; 野口 哲子; 岡田 茂, 第33回植物細胞分子生物学会, Botryococcus braunii B品種におけるスクアレンエポキシダーゼ遺伝子群の\n単離と機能解析, 2015年08月, 日本語, 東京, 国内会議
• 三井菜々香; 岡田茂; 野口哲子; 鍵和田聡, 日本植物学会第78回年会, 緑藻Botryococcus由来炭化水素（Botryococcene）生産酵母での細胞内脂質蓄積部位の形態, 2014年09月, 日本語, 川崎市, 国内会議
• 三井菜々香; 野口哲子; 鍵和田聡, 日本植物学会第77回大会, 緑藻Botryococcus brauniiのトリテルペン合成酵素群の出芽酵母での発現と細胞内局在, 2013年09月, 日本語, 札幌, 国内会議
• 鍵和田聡; Suzuki, R; Uno, Y; Nishii, I; Kagiwada, S; Noguchi, T, 10th International Phycological Congress Annual Meeting, Biosynthesis and accumulation of hydrocarbons and polysaccharides in a colonial green alga, Botryococcus braunii race B, 2013年08月, 英語, Orlando, USA, 国際会議
• 鍵和田聡, 第54回日本植物生理学会年会, Biosynthesis and accumulation of lipids and polysaccharides in a colonial\ngreen alga, Botryococcus braunii race B, 2013年03月, 英語, 国内会議
• 大島彩加; 横井梨紗; 増田実希; 鍵和田聡, 第35回日本分子生物学会年会, 出芽酵母Saccharomyces cerevisiaeのリン脂質合成遺伝に発現に関与するOpi1p転写因子の量は膜への結合により調節される, 2012年12月, 日本語, 福岡, 国内会議
• 三宅和; 中道晶子; 鍵和田聡, 第35回日本分子生物学会年会, 出芽酵母(Saccharomyces cerevisiae)のALS8型Scs2pが引き起こす多重膜小胞体はリン脂質組成の変化により解消できる, 2012年12月, 日本語, 福岡, 国内会議
• 鈴木玲子; 宇野由紀; 鍵和田聡; 西井一郎; 野口哲子, 日本植物学会第76回大会, 緑藻Botryococcus braunii Ｂ品種における脂質の分泌に伴うオイルボディの動態, 2012年09月, 日本語, 兵庫県姫路市, 国内会議
• 鍵和田聡, The 2nd International Conference on Algal Biomass, Biofuels and Bioproducts, Hydrocarbon biosynthesis and secretion in a green alga, Botryococcus braunii, 2012年06月, 英語, San Diego, USA, 国際会議
• 増田実希; 中道晶子; 鍵和田聡, 第34回日本分子生物学会年会, 出芽酵母のイノシトール生合成遺伝子INO1の負の転写因子Opi1pの局在, 2011年12月, 日本語, 横浜市みなとみらい, 国内会議
• 大島彩加; 中道晶子; 横井梨紗; 鍵和田聡, 第34回日本分子生物学会年会, 出芽酵母のリン脂質合成遺伝子の負の転写調節因子OPI1の発現は、Opi1pに結合する核/小胞体膜タンパク質Scs2pにより調節される, 2011年12月, 日本語, 横浜市みとみらい
• 伊藤奈央子; 宇野由紀; 西井一郎; 鍵和田聡; 野口哲子, 日本植物形態学会第23回大会, 緑藻Botryococcus braunii B品種における炭化水素生産について, 2011年09月, 日本語
• 家田七彩; 鍵和田聡, 日本分子生物学会, 出芽酵母Saccharomyces cerevisiaeにおいてeisosome構成タンパ ク質Pil1pが形成する細胞内紐状構造の成因, 2021年12月03日, 2021年12月01日, 2021年12月03日, 日本語
• 坂巻美羽; 鍵和田聡, 日本農芸化学会2023年度大会, 緑藻Botryococcus braunii由来ボツリオコッセン合成酵素Ssl- 3pの酵素活性向上, 2023年03月14日, 2023年03月14日, 2023年03月17日, その他
• 池田亜衣梨; 鍵和田聡, 第46回日本分子生物学会年会, 出芽酵母 Saccharomyces cerevisiae の スクアレン 合成活性 が 膜接触部位 に 与える 影響, 2022年12月01日, その他

共同研究・競争的資金等の研究課題

• 生体膜の構造と機能に関する分子生物学的研究, 0, 0, 0, 競争的資金
• Structure and function of biomembrane, 0, 0, 0, 競争的資金
• 基盤研究(C), 2022年04月, 2025年03月, 22K04842, 細胞壁自己消化性酵母を用いたスクアレン回収システムの構築, 鍵和田 聡, 日本学術振興会, 科学研究費助成事業 基盤研究(C), 奈良女子大学, 4290000, 3300000, 990000, kaken

  対象リソースIRI