Researchers Database

SAKAI Atsushi

    Faculty Division of Natural Sciences Research Group of Biological Sciences Professor
    Education and Resesrch Council Education and Resesrch Councilors
Contact:
sakaicc.nara-wu.ac.jp
Last Updated :2021/10/27

researchmap

Degree

  • (BLANK), The University of Tokyo
  • (BLANK), The University of Tokyo

Research Interests

  • photosynthesis, respiration, plastids, mitocondria, interaction among organisms, allelopathy, defense against herbibors, defense against pathogens, thigmomorphogenesis 

Research Areas

  • Life sciences, Plants: molecular biology and physiology

Research Experience

  • Apr. 2014, 奈良女子大学研究院自然科学系・教授
  • Apr. 1999 Mar. - 2014, 奈良女子大学理学部助教授/准教授
  • Aug. 1991 Mar. - 1999, 東京大学理学部助手

Education

  • - 1991, The University of Tokyo, Graduate School, Division of Science, 植物学
  • - 1989, The University of Tokyo, Faculty of Science, 生物学科植物学教室

Committee Memberships

  • 日本植物生理学会会計幹事
  • 日本植物形態学会庶務幹事
  • 日本植物形態学会評議員
  • 日本植物形態学会大会準備委員長
  • Apr. 2020The Japan Mendel Societycouncillor

Awards

  • キトロギア奨励賞, 酒井敦, 日本メンデル協会, 2014
  • 日本植物学会奨励賞, 1999

Published Papers

  • C3-Like Photosynthetic Properties of Senescing Maize Leaves Are Accompanied by Preferential Senescence of Mesophyll Cells

    Saya Shiogai; Satoshi Tamotsu; Atsushi Sakai

    International Society of Cytology, 25 Dec. 2018, CYTOLOGIA, 83 (4), 387 - 391, doi;url

    Scientific journal

  • Cytological Studies on Proliferation, Differentiation, and Death of BY-2 Cultured Tobacco Cells

    Atsushi Sakai; Mari Takusagawa; Asuka Nio; Yu Sawai

    International Society of Cytology, Jun. 2015, CYTOLOGIA, 80 (2), 133 - 141, doi;web_of_science

    Scientific journal

  • Phototaxis of sacoglossan sea slugs with different photosynthetic abilities: a test of the ‘crawling leaves’ hypothesis

    Ayaka Miyamoto; Atsushi Sakai; Rie Nakano; Yoichi Yusa

    Springer Science and Business Media LLC, Jun. 2015, Marine Biology, 162 (6), 1343 - 1349, doi;url;url;url

    Scientific journal

  • Relative importance and interactive effects of photosynthesis and food in two solar-powered sea slugs

    Ayana Akimoto; Yayoi M. Hirano; Atsushi Sakai; Yoichi Yusa

    Sacoglossans use chloroplasts taken from algal food for photosynthesis (kleptoplasty), but the adaptive significance of this phenomenon remains unclear. Two con-generic sacoglossans (Elysia trisinuata and E. atroviridis) were collected in 2009-2011 from Shirahama (33.69A degrees N, 135.34A degrees E) and Mukaishima (34.37A degrees N, 133.22A degrees E), Japan, respectively. They were individually maintained for 16 days under four experimental conditions (combination of light/dark and with/without food), and their survival rate and relative (=final/initial) weights were measured. Both light and food had positive effects on the survival in E. trisinuata, whereas no positive effects of light or food on survival were detected in E. atroviridis. Both light and food had positive effects on relative weights in both species, but light had smaller effects than food. A significant interaction term between light and food was detected in E. trisinuata (but not in E. atroviridis) in that only the presence of both resulted in weight gains. This result suggests that E. trisinuata can obtain sufficient additional energy from photosynthesis for sustaining growth when fresh chloroplasts are continuously supplied from algal food. In addition, fluorescence yield measurements showed that unfed individuals of both E. trisinuata and E. atroviridis lost photosynthetic activity soon (< 4 and 4-8 days, respectively). In conclusion, photosynthesis may function to obtain supplementary nutrition for sustaining growth when food is available in sacoglossans with short-term functional kleptoplasty., SPRINGER, May 2014, MARINE BIOLOGY, 161 (5), 1095 - 1102, doi;web_of_science

    Scientific journal

  • Effects of Salinity on the Growth and Survival of the Seedlings of Mangrove, Rhizophora stylosa

    Hiromi Kanai; Mitsuki Tajima; Atsushi Sakai

    Sciencedomain International, 10 Jan. 2014, International Journal of Plant & Soil Science, 3 (7), 879 - 893, doi;url

    Scientific journal

  • Histone H3 is absent from organelle nucleoids in BY-2 cultured tobacco cells

    Mari Takusagawa; Satoshi Tamotsu; Atsushi Sakai

    Wiley, Jul. 2013, Cell Biology International, 37 (7), 748 - 754, doi;web_of_science;url

    Scientific journal

  • Effects of chloroplast dysfunction on mitochondria: white sectors in variegated leaves have higher mitochondrial DNA levels and lower dark respiration rates than green sectors

    Haruka Toshoji; Tomomi Katsumata; Mari Takusagawa; Yoichi Yusa; Atsushi Sakai

    Springer Science and Business Media LLC, Oct. 2011, Protoplasma, 249 (3), 805 - 817, doi;web_of_science;url

    Scientific journal

  • Monoterpenes of Salvia leucophylla

    Atsushi Sakai; Hiroko Yoshimura

    Bentham Science Publishers Ltd., 02 Apr. 2012, Current Bioactive Compounds, 8 (1), 90 - 100, doi;url

    Scientific journal

  • Theca Cell Layer Formation in Mouse Ovarian Follicle Culture in vitro

    Saori Itami; Keiko Yasuda; Satoshi Tamotsu; Atsushi Sakai

    UNIV TOKYO CYTOLOGIA, Sep. 2012, CYTOLOGIA, 77 (3), 288 - 288, web_of_science

    Scientific journal

  • Effects of photosynthesis on the survival and weight retention of two kleptoplastic sacoglossan opisthobranchs

    Shoko Yamamoto; Yayoi M. Hirano; Yoshiaki J. Hirano; Cynthia D. Trowbridge; Ayana Akimoto; Atsushi Sakai; Yoichi Yusa

    Many sacoglossan sea slugs utilize chloroplasts ingested from food algae for photosynthesis (functional kleptoplasty), and the extent and duration of kleptoplast retention differs greatly among sacoglossan species. Although most recent studies focus on the genetic, microscopic, or physiological mechanisms responsible for this unique phenomenon, its effects on the life history traits of sacoglossans have not been fully explored. To study the effects of light conditions on survival and weight retention, adult individuals of two sacoglossan species, Elysia trisinuata and Plakobranchus ocellatus (‘black type'), were reared under light conditions (a 14-hour light: 10-hour dark photoperiod with an irradiance level of 28 µmol m−2s−1) or complete darkness for 21 days. There was no significant difference in the survival rate between the light and dark treatments for E. trisinuata, and its wet weight relative to the initial weight was smaller in the light than in the dark. However, both the survival and relative weights were greater in the light than dark for P. ocellatus. Based on the fluorescent yield measurement using pulse-amplitude-modulated fluorometry, the retention duration of functional chloroplasts was longer (>17 days) for P. ocellatus than E. trisinuata (<4 days). These results indicate that P. ocellatus benefits from photosynthesis for survival and growth, whereas E. trisinuata does not under starved conditions. This interspecific difference is likely related to the period of functional chloroplast retention., Cambridge University Press (CUP), Feb. 2013, Journal of the Marine Biological Association of the United Kingdom, 93 (1), 209 - 215, doi;url

    Scientific journal

  • The Roles of THY1 and Integrin Beta3 in Cell Adhesion During Theca Cell Layer Formation and the Effect of Follicle-Stimulating Hormone on THY1 and Integrin Beta3 Localization in Mouse Ovarian Follicles

    Saori Itami; Satoshi Tamotsu; Atsushi Sakai; Keiko Yasuda

    Oxford University Press (OUP), 01 May 2011, Biology of Reproduction, 84 (5), 986 - 995, doi;web_of_science

    Scientific journal

  • 1,8-Cineole Inhibits Both Proliferation and Elongation of BY-2 Cultured Tobacco Cells

    Hiroko Yoshimura; Yu Sawai; Satoshi Tamotsu; Atsushi Sakai

    Springer Science and Business Media LLC, Feb. 2011, Journal of Chemical Ecology, 37 (3), 320 - 328, doi;web_of_science;url

    Scientific journal

  • Co-culturing of follicles with interstitial cells in collagen gel reproduce follicular development accompanied with theca cell layer formation

    Saori Itami; Keiko Yasuda; Yuka Yoshida; Chiyuki Matsui; Sachie Hashiura; Atsushi Sakai; Satoshi Tamotsu

    Springer Science and Business Media LLC, Dec. 2011, Reproductive Biology and Endocrinology, 9 (1), 159 - 159, doi;web_of_science

    Scientific journal

  • Organization of Mitochondrial-Nucleoids in BY-2 Cultured Tobacco Cells

    Mari Takusagawa; Tomomi Hayashi; Hiroyoshi Takano; Atsushi Sakai

    International Society of Cytology, Sep. 2009, CYTOLOGIA, 74 (3), 329 - 341, doi

    Scientific journal

  • Effects of Growth Phase and Cell Density on Cryptogein-induced Programmed Cell Death in Suspension-cultured Tobacco BY-2 Cells: Development of a Model System for 100% Efficient Hypersensitive Cell Death

    Yu Sawai; Satoshi Tamotsu; Kazuyuki Kuchitsu; Atsushi Sakai

    International Society of Cytology, Dec. 2010, CYTOLOGIA, 75 (4), 389 - 396, doi;web_of_science

    Scientific journal

  • Effects of chloroplast dysfunction in a subpopulation of leaf mesophyll cells on photosynthetic and respiratory activities of a whole leaf: A study using variegated leaves of Hedera helix L.

    Naoko Yoshioka; Yuki Imanishi; Keiko Yasuda; Atsushi Sakai

    The Japanese Society of Plant Morphology, Dec. 2009, PLANT MORPHOLOGY, 21 (1), 87 - 91, doi

    Scientific journal

  • NtPolI-like1 and NtPolI-like2, Bacterial DNA Polymerase I Homologs Isolated from BY-2 Cultured Tobacco Cells, Encode DNA Polymerases Engaged in DNA Replication in Both Plastids and Mitochondria

    Y. Ono; A. Sakai; K. Takechi; S. Takio; M. Takusagawa; H. Takano

    Oxford University Press (OUP), 17 Oct. 2007, Plant and Cell Physiology, 48 (12), 1679 - 1692, doi;web_of_science;url

    Scientific journal

  • Tobacco BY-2 Cells as a Model for Differentiation in Heterotrophic Plant Cells

    Y. Miyazawa; A. Sakai

    Springer Berlin Heidelberg, 2006, Tobacco BY-2 Cells: From Cellular Dynamics to Omics, 119 - 132, doi;url

    In book

  • Changes in the Distribution of Tenascin and Fibronectin in the Mouse Ovary During Folliculogenesis, Atresia, Corpus Luteum Formation and Luteolysis

    Keiko Yasuda; Emi Hagiwara; Akiko Takeuchi; Chinatsu Mukai; Chiyuki Matsui; Atsushi Sakai; Satoshi Tamotsu

    Zoological Society of Japan, Feb. 2005, Zoological Science, 22 (2), 237 - 245, doi;web_of_science;url

    Scientific journal

  • Allelopathic Effects of Volatile Monoterpenoids Produced by Salvia leucophylla: Inhibition of Cell Proliferation and DNA Synthesis in the Root Apical Meristem of Brassica campestris Seedlings

    Nami Nishida; Satoshi Tamotsu; Noriko Nagata; Chieko Saito; Atsushi Sakai

    Springer Science and Business Media LLC, May 2005, Journal of Chemical Ecology, 31 (5), 1187 - 1203, doi;web_of_science;url;url;url

    Scientific journal

  • Profiling of Bryophyte gene expression by hybridization of an Arabidopsis cDNA array with Bryophyte cDNA.

    SUNG JIN CHUNG; KATSUAKI TAKECHI; ATSUSHI SAKAI; KANJI ONO; HIROYOSHI TAKANO

    Jan. 2006, Journal of Hattori Botanical Laboratory, 99, 233 - 244

    Scientific journal

  • Detection of genes differentially expressed in cultured cells of Marchantia polymorpha, but not in Arabidopsis thaliana, using an Arabidopsis cDNA microarray.

    Sung Jin Chung; Katsuaki Takechi; Atsushi Sakai; Kanji Ono; Hiroyoshi Takano

    Jan. 2006, Journal of Hattori Botanical Laboratory, 99, 245 - 247

    Scientific journal

  • Studies on Dynamic Changes of Organelles Using Tobacco BY-2 as the Model Plant Cell Line

    Atsushi Sakai; Yutaka Miyazawa; Tsuneyoshi Kuroiwa

    Springer Berlin Heidelberg, 2004, Tobacco BY-2 Cells, 192 - 216, doi;url

    In book

  • Organelle nuclei in higher plants: Structure, composition, function, and evolution

    Atsushi Sakai; Hiroyoshi Takano; Tsuneyoshi Kuroiwa

    Plant cells have two distinct types of energy-converting organelles: plastids and mitochondria. These organelles have their own DNAs and are regarded as descendants of endosymbiotic prokaryotes. The organelle DNAs associate with various proteins to form compact DNA-protein complexes, which are referred to as organelle nuclei or nucleoids. Various functions of organelle genomes, such as DNA replication and transcription, are performed within these compact structures. Fluorescence microscopy using the DNA-specific fluorochrome 4′,6-diamidino-2-phenylindole has played a pivotal role in establishing the concept of "organelle nuclei." This fluorochrome has also facilitated the isolation of morphologically intact organelle nuclei, which is indispensable for understanding their structure and composition. Moreover, development of an in vitro transcriptionDNA synthesis system using isolated organelle nuclei has provided us with a means of measuring and analyzing the function of organelle nuclei. In addition to these morphological and biochemical approaches, genomics has also had a great impact on our ability to investigate the components of organelle nuclei. These analyses have revealed that organelle nuclei are not a vestige of the bacterial counterpart, but rather are a complex system established through extensive interaction between organelle and cell nuclear genomes during evolution. Extensive diversion or exchange during evolution is predicted to have occurred for several important structural proteins, such as major DNA-compacting proteins, and functional proteins, such as RNA and DNA polymerases, resulting in complex mechanisms to control the function of organelle genomes. Thus, organelle nuclei represent the most dynamic front of interaction between the three genomes (cell nuclear, plastid, and mitochondrial) constituting eukaryotic plant cells. © 2004 Elsevier Inc., Jan. 2004, International Review of Cytology, 238, 59 - 118, doi;pubmed

    In book

  • Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan Synthesis Pathway on Moss Chloroplast Division

    Nami Katayama; Hiroyoshi Takano; Motoji Sugiyama; Susumu Takio; Atsushi Sakai; Kan Tanaka; Haruko Kuroiwa; Kanji Ono

    Oxford University Press (OUP), 15 Jul. 2003, Plant and Cell Physiology, 44 (7), 776 - 781, doi;web_of_science;url

    Scientific journal

  • Glom Is a Novel Mitochondrial DNA Packaging Protein inPhysarum polycephalumand Causes Intense Chromatin Condensation without Suppressing DNA Functions

    Narie Sasaki; Haruko Kuroiwa; Chikako Nishitani; Hiroyoshi Takano; Tetsuya Higashiyama; Tamaki Kobayashi; Yuki Shirai; Atsushi Sakai; Shigeyuki Kawano; Kimiko Murakami-Murofushi; Tsuneyoshi Kuroiwa

    Mitochondrial DNA (mtDNA) is packed into highly organized structures called mitochondrial nucleoids (mt-nucleoids). To understand the organization of mtDNA and the overall regulation of its genetic activity within the mt-nucleoids, we identified and characterized a novel mtDNA packaging protein, termed Glom (a protein inducing agglomeration of mitochondrial chromosome), from highly condensed mt-nucleoids of the true slime mold, Physarum polycephalum. This protein could bind to the entire mtDNA and package mtDNA into a highly condensed state in vitro. Immunostaining analysis showed that Glom specifically localized throughout the mt-nucleoid. Deduced amino acid sequence revealed that Glom has a lysine-rich region with proline-rich domain in the N-terminal half and two HMG boxes in C-terminal half. Deletion analysis of Glom revealed that the lysine-rich region was sufficient for the intense mtDNA condensation in vitro. When the recombinant Glom proteins containing the lysine-rich region were expressed in Escherichia coli, the condensed nucleoid structures were observed in E. coli. Such in vivo condensation did not interfere with transcription or replication of E. coli chromosome and the proline-rich domain was essential to keep those genetic activities. The expression of Glom also complemented the E. coli mutant lacking the bacterial histone-like protein HU and the HMG-boxes region of Glom was important for the complementation. Our results suggest that Glom is a new mitochondrial histone-like protein having a property to cause intense DNA condensation without suppressing DNA functions., American Society for Cell Biology (ASCB), Dec. 2003, Molecular Biology of the Cell, 14 (12), 4758 - 4769, doi;web_of_science

    Scientific journal

  • Activation of cell proliferation by brassinolide application in tobacco BY-2 cells: effects of brassinolide on cell multiplication, cell-cycle-related gene expression, and organellar DNA contents

    Yutaka Miyazawa; Naoko Nakajima; Tomoko Abe; Atsushi Sakai; Shozo Fujioka; Shigeyuki Kawano; Tsuneyoshi Kuroiwa; Shigeo Yoshida

    Oxford University Press (OUP), 29 Oct. 2003, Journal of Experimental Botany, 54 (393), 2669 - 2678, doi;web_of_science;url

    Scientific journal

  • An obligate-halophytic mangrove, Rhizophora mucronate, does not require Na+ for the uptake of nutrient ions in their roots

    Hiromi Kanai; Atsushi Sakai

    Elsevier BV, Feb. 2021, Aquatic Botany, 169, 103328 - 103328, doi

    Scientific journal

  • The maize coleoptiles do not perform typical C4 photosynthesis: investigation with special reference to anatomy, photosynthetic property, and gene expression

    Yasuko Fukaya; Naoko Yoshioka; Hitoshi Sakakibara; Carlos S. Andreo; Tadahiko Mae; Keiko Yasuda; Atsushi Sakai

    The Japanese Society of Plant Morphology, 2012, PLANT MORPHOLOGY, 24 (1), 111 - 121, doi

    Scientific journal

  • Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2

    Yutaka Miyazawa; Atsushi Sakai; Sachihiro Matsunaga; Tadao Asami; Shigeyuki Kawano; Tsuneyoshi Kuroiwa; Shigeo Yoshida

    Oxford University Press (OUP), 01 Dec. 2002, Journal of Experimental Botany, 53 (379), 2451 - 2452, doi;web_of_science;url

    Scientific journal

  • Ampicillin Inhibits Chloroplast Division in Cultured Cells of the Liverwort Marchantia polymorpha.

    Eiichirou Tounou; Susumu Takio; Atsushi Sakai; Kanji Ono; Hiroyoshi Takano

    International Society of Cytology, 2002, CYTOLOGIA, 67 (4), 429 - 434, doi

    Scientific journal

  • Angiosperm species that produce sperm cell pairs or generative cells with polarized distribution of DNA-containing organelles

    Chieko Saito; Noriko Nagata; Atsushi Sakai; Kimie Mori; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, Dec. 2002, Sexual Plant Reproduction, 15 (4), 167 - 178, doi;web_of_science;url;url;url

    Scientific journal

  • Activation of organelle DNA synthesis during the initial phase of proliferation of BY-2 cultured tobacco cells after medium renewal

    Saori Okamura; Takeshi Suzuki; Yutaka Miyazawa; Tsuneyoshi Kuroiwa; Atsushi Sakai

    The Japanese Society of Plant Morphology, Dec. 2002, PLANT MORPHOLOGY, 14 (1), 16 - 28, doi

    Scientific journal

  • Three-Dimensional Progression of Programmed Death in the Rice Coleoptile

    Noriko Inada; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Elsevier, 2002, International Review of Cytology, 221 - 260e, doi;web_of_science

    In book

  • Differential regulation of starch synthesis gene expression during amyloplast development in cultured tobacco BY-2 cells

    Yutaka Miyazawa; Atsushi Sakai; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Elsevier BV, Jan. 2001, Journal of Plant Physiology, 158 (8), 1077 - 1084, doi;web_of_science

    Scientific journal

  • In vitro Transcription/DNA Synthesis Using Isolated Organelle-nuclei: Application to the Analysis of the Mechanisms that Regulate Organelle Genome Function

    Atsushi Sakai

    Springer Science and Business Media LLC, Jun. 2001, Journal of Plant Research, 114 (2), 199 - 211, doi;web_of_science;url;url;url

    Scientific journal

  • Behavior of plastid nucleoids during male gametogenesis in Plumbago auriculata

    C Saito; N Nagata; A Sakai; H Kuroiwa; T Kuroiwa

    We characterized the behavior of plastid (pt) and mitochondrial(mt) nucleoids during male gametogenesis in Plumbago auriculata in three dimensions. The behavior of pt-nucleoids and mt-nucleoids differed throughout male gametogenesis. Pt-nucleoids were distributed in a characteristic manner in three stages: in the early microspore, pt-nucleoids assemble around cell nucleus: in the mid-generative cell, pt-nucleoids gather at the internal side of the pollen: in the late-generative cell, pt-nucleoids aggregation turns its pole to the external side of the pollen. We also studied organelle nucleoids in the egg and the central cell by a method in which semi thick sections of resin-embedded anthers and ovaries were observed by confocal laser scanning microscopy. The number of pt-nucleoids in the sperm cell did not differ significantly from that in the egg. These results suggest that the behavior of DNA-containing organelles is regulated strictly during male gametogenesis in P. auriculata, and that a biparental inheritance of plastids in the Plumbago embryo is more favored than was previously thought., SPRINGER-VERLAG WIEN, 2001, PROTOPLASMA, 216 (3-4), 143 - 154, web_of_science

    Scientific journal

  • A putative mitochondrial ftsZ gene is present in the unicellular primitive red alga Cyanidioschyzon merolae

    M. Takahara; H. Takahashi; S. Matsunaga; S. Miyagishima; H. Takano; A. Sakai; S. Kawano; T. Kuroiwa

    Springer Science and Business Media LLC, Nov. 2000, Molecular Genetics and Genomics, 264 (4), 452 - 460, doi;web_of_science;url;url;url

    Scientific journal

  • Unequal distribution of DNA-containing organelles in generative and sperm cells of Erythrina crista-galli (Fabaceae)

    C. Saito; N. Nagata; A. Sakai; K. Mori; H. Kuroiwa; T. Kuroiwa

    Springer Science and Business Media LLC, 14 Apr. 2000, Sexual Plant Reproduction, 12 (5), 296 - 301, doi;web_of_science;url;url;url

    Scientific journal

  • Isolation, characterization, and chromosomal mapping of an ftsZ gene from the unicellular primitive red alga Cyanidium caldarium RK-1

    Manabu Takahara; Hidenori Takahashi; Sachihiro Matsunaga; Atsushi Sakai; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, 23 Feb. 2000, Current Genetics, 37 (2), 143 - 151, doi;web_of_science;url;url;url

    Scientific journal

  • Unique positioning of mitochondria in developing microspores and pollen grains inPharbitis nil: mitochondria cover the nuclear surface at specific developmental stages

    N. Nagata; C. Saito; A. Sakai; H. Kuroiwa; T. Kuroiwa

    Springer Science and Business Media LLC, Mar. 2000, Protoplasma, 213 (1-2), 74 - 82, doi;web_of_science;url;url;url

    Scientific journal

  • Organellar Protein Synthesis Controls Amyloplast Formation Independent of Starch Synthesis Gene Expression.

    Yutaka Miyazawa; Atsushi Sakai; Shigeyuki Kawano; Tsuneyoshi Kuroiwal

    The transfer of stationary-phase cultured tobacco (Nicotiana tabacum L.) BY-2 cells into auxin-depleted culture medium induces amyloplast formation. To investigate the timing and requirement for de novo protein synthesis in organelles during amyloplast development and the enhancement of starch synthesis gene expression, we added chloramphenicol, at various times, to cells grown in amyloplast-inducing medium. Changes in cell growth, starch accumulation, and the mRNA levels of the ADP-glucose pyrophosphorylase small subunit (AgpS) gene Were monitored. Chloramphnicol inhibited starch accumulation, but had no significant effect on cell growth, irrespective of the time of addition. RNA gel-blot analyses revealed that chloramphenicol treatment did not reduce the accumulation of mRNA from the AgpS gene, irrespective of addition time. These results suggest that organellar protein synthesis affects starch accumulation independently of starch synthesis gene expression. The necessity of organellar gene expression for starch accumulation is also discussed., International Society of Cytology, Dec. 2000, CYTOLOGIA, 65 (4), 435 - 442, doi

    Scientific journal

  • Isolation of chloroplasts and chloroplast-nuclei(nucleoids)from Chlamydomonas reinhardtii

    Atsushi Sakai; Lena Suzuki; Osami Misumi; Tetsuya Higashiyama; Tsuneyoshi Kuroiwa

    The Japanese Society of Plant Morphology, Dec. 2000, PLANT MORPHOLOGY, 12 (1), 2 - 9, doi

    Scientific journal

  • Auxin and Cytokinin Have Opposite Effects on Amyloplast Development and the Expression of Starch Synthesis Genes in Cultured Bright Yellow-2 Tobacco Cells

    Yutaka Miyazawa; Atsushi Sakai; Shin-ya Miyagishima; Hiroyoshi Takano; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Oxford University Press (OUP), 01 Oct. 1999, Plant Physiology, 121 (2), 461 - 470, doi;web_of_science;url

    Scientific journal

  • Two Types of ftsZ Genes Isolated from the Unicellular Primitive Red Alga Galdieria sulphuraria

    M. Takahara; H. Takahashi; S. Matsunaga; A. Sakai; S. Kawano; T. Kuroiwa

    Oxford University Press (OUP), 01 Jan. 1999, Plant and Cell Physiology, 40 (8), 784 - 791, doi;web_of_science;url

    Scientific journal

  • The selective increase or decrease of organellar DNA in generative cells just after pollen mitosis one controls cytoplasmic inheritance

    Noriko Nagata; Chieko Saito; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, 19 Jul. 1999, Planta, 209 (1), 53 - 65, doi;web_of_science;url;url;url

    Scientific journal

  • Decrease in mitochondrial DNA and concurrent increase in plastid DNA in generative cells of Pharbitis nil during pollen development

    Noriko Nagata; Chieko Saitoa; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Elsevier BV, Apr. 1999, European Journal of Cell Biology, 78 (4), 241 - 248, doi;web_of_science

    Scientific journal

  • Amyloplast formation in cultured tobacco cells. III Determination of the timing of gene expression necessary for starch accumulation

    A. Sakai; Y. Miyazawa; C. Saito; N. Nagata; H. Takano; H.-Y. Hirano; T. Kuroiwa

    Springer Science and Business Media LLC, 09 Mar. 1999, Plant Cell Reports, 18 (7-8), 589 - 594, doi;web_of_science;url;url;url

    Scientific journal

  • Plastid Gene Expression during Amyloplast Formation in Cultured Tobacco Cells

    Atsushi Sakai; Yutaka Miyazawa; Takeshi Suzuki; Narie Sasaki; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Elsevier BV, Jan. 1999, Journal of Plant Physiology, 154 (1), 71 - 78, doi;web_of_science

    Scientific journal

  • Comparative analysis of DNA synthesis activity in plastid-nuclei and mitochondrial-nuclei simultaneously isolated from cultured tobacco cells

    Atsushi Sakai; Takeshi Suzuki; Noriko Nagata; Narie Sasaki; Yutaka Miyazawa; Chieko Saito; Noriko Inada; Yoshiki Nishimura; Tsuneyoshi Kuroiwa

    Elsevier BV, Jan. 1999, Plant Science, 140 (1), 9 - 19, doi;web_of_science

    Scientific journal

  • Semi-Automatic Laser Beam Microdissection of the Y Chromosome and Analysis of Y Chromosome DNA in a Dioecious Plant, Silene latifolia

    S. Matsunaga; S. Kawano; T. Michimoto; T. Higashiyama; S. Nakao; A. Sakai; T. Kuroiwa

    Oxford University Press (OUP), 01 Jan. 1999, Plant and Cell Physiology, 40 (1), 60 - 68, doi;web_of_science;url

    Scientific journal

  • Improved Sensitivity for High Resolution in Situ Hybridization Using Resin Extraction of Methyl Methacrylate Embedded Material

    Chieko Saito; Makoto Hay Ashi; Atsushi Sakai; Makoto Fujie; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Informa UK Limited, Jan. 1999, Biotechnic & Histochemistry, 74 (1), 40 - 48, doi;web_of_science;url

    Scientific journal

  • Senescence program in rice (Oryza sativa L.) leaves: analysis of the blade of the second leaf at the tissue and cellular levels

    N Inada; A Sakai; H Kuroiwa; T Kuroiwa

    Previously, we showed that all greening mesophyll cells in the coleoptiles of rice (Oryza sativa L. cv. Nippon-bare) follow the identical program of senescence, which features the early degradation of chloroplast DNA (cpDNA) and subsequent nuclear condensation and disorganization. Following the coleoptile study, we analyzed the senescence-associated changes in the blade of the second leaf of rice at the tissue and cellular levels. Under the experimental conditions, the second leaf started to elongate rapidly 2 days after sowing and emerged on day 3. The blade of the second leaf completed its growth on day 4, although the sheath continued to grow until day 7. The amount of soluble protein and chlorophyll (Chl) per blade reached a maximum on day 7, and then declined. When blades were divided into three parts (the tip, mid-region, and base), levels of both soluble protein and Chl in the tip segment peaked earlier and decreased at a faster rate than in the other parts, demonstrating a longitudinal gradient of senescence from the tip to the base of the blade. In cross sections through the center of the tip and base segments, all the mesophyll cells senesced synchronously. They passed through the following steps in order: (i) degradation of cpDNA, (ii) decrease in the size of the chloroplast with degeneration of the chloroplast inner membranes, and (iii) condensation and disorganization of the nuclei. Although some differences were shown between the coleoptile and the second leaf in the timing and rate of each event, the order of those senescence-related events was conserved, suggesting an identical program of senescence exists in rice leaves., SPRINGER-VERLAG WIEN, 1999, PROTOPLASMA, 207 (3-4), 222 - 232, web_of_science

    Scientific journal

  • Isolation and phenotypic characterization of Chlamydomonas reinhardtii mutants defective in chloroplast DNA segregation

    O Misumi; L Suzuki; Y Nishimura; A Sakai; S Kawano; H Kuroiwa; T Kuroiwa

    Each wild-type Chlamydomonas reinhardtii cell has one large chloroplast containing several nuclei (nucleoids). We used DNA insertional mutagenesis to isolate Chlamydomonas mutants which contain a single, large chloroplast (cp) nucleus and which we named moc (monokaryotic chloroplast). DAPI-fluorescence microscopy and microphotometry observations revealed that moc mutant cells only contain one cp-nucleus throughout the cell division cycle, and that unequal segregation of cpDNA occurred during cell division in the moc mutant. One cell with a large amount of cpDNA and another with a small amount of cpDNA were produced after the first cell division. Unequal segregation also occurred in the second cell division, producing one cell with a large amount (about 70 copies) of cpDNA and three other cells with a small amount (only 2-8 copies) of cpDNA, However, most individual moc cells contained several dozen cpDNA copies 12 h after the completion of cell division, suggesting that cpDNA synthesis was activated immediately after chloroplast division. In contrast to the cpDNA, the mitochondrial (mt) DNA of the moc mutants was observed as tiny granules scattered throughout the entire cell. These segregated to each daughter cell equally during cell division. Electron-microscopic observation of the ultrastructure of moc mutants showed that a low-electron-density area, which was identified as the cp-nucleus by immunoelectron microscopy with anti-DNA antibody, existed near the pyrenoid. However, there were no other structural differences between the chloroplasts of wild-type cells and moc mutants. The thylakoid membranes and pyrenoid were identical. Therefore, we propose that the novel moc mutants are only defective in the dispersion and segregation of cpDNA. This strain should be useful to elucidate the mechanism for the segregation of cpDNA., SPRINGER WIEN, 1999, PROTOPLASMA, 209 (3-4), 273 - 282, web_of_science

    Scientific journal

  • Three-dimensional analysis of the senescence program in rice ( Oryza sativa L.) coleoptiles

    Noriko Inada; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, 07 Oct. 1998, Planta, 206 (4), 585 - 597, doi;web_of_science;url;url;url

    Scientific journal

  • Transcriptional Activities of the Chloroplast-Nuclei and Proplastid-Nuclei Isolated from Tobacco Exhibit Different Sensitivities to Tagetitoxin: Implication of the Presence of Distinct RNA Polymerases

    A. Sakai; C. Saito; N. Inada; T. Kuroiwa

    Oxford University Press (OUP), 01 Sep. 1998, Plant and Cell Physiology, 39 (9), 928 - 934, doi;web_of_science;url

    Scientific journal

  • Three-dimensional analysis of the senescence program in rice ( Oryza sativa L.) coleoptiles

    Noriko Inada; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, 18 May 1998, Planta, 205 (2), 153 - 164, doi;web_of_science;url;url;url

    Scientific journal

  • Comparative Analysis of Plastid Gene Expression in Tobacco Chloroplasts and Proplastids: Relationship between Transcription and Transcript Accumulation

    A. Sakai; T. Suzuki; Y. Miyazawa; S. Kawano; T. Nagata; T. Kuroiwa

    Oxford University Press (OUP), 01 Jun. 1998, Plant and Cell Physiology, 39 (6), 581 - 589, doi;web_of_science;url

    Scientific journal

  • Isolation, characterization, and chromosome mapping of an actin gene from the primitive green alga, Nannochloris bacillaris (Chlorophyceae)

    Sayaka Arai; Hidenori Takahashi; Hiroyoshi Takano; Atsushi Sakai; Shigeyuki Kawano

    Wiley, Jun. 1998, Journal of Phycology, 34 (3), 477 - 485, doi;web_of_science;url

    Scientific journal

  • Simultaneous isolation of cell-nuclei, plastid-nuclei and mitochondrial-nuclei from cultured tobacco cells; comparative analysis of their transcriptional activities in vitro

    Atsushi Sakai; Takeshi Suzuki; Yutaka Miyazawa; Tsuneyoshi Kuroiwa

    Elsevier BV, Apr. 1998, Plant Science, 133 (1), 17 - 31, doi;web_of_science

    Scientific journal

  • Detection and quantification of rRNA by high-resolutionin situ hybridization in pollen grains

    Chieko Saito; Makoto Fujie; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, Mar. 1998, Journal of Plant Research, 111 (1), 45 - 52, doi;web_of_science;url;url;url

    Scientific journal

  • The Division Apparatus of Plastids and Mitochondria

    Tsuneyoshi Kuroiwa; Haruko Kuroiwa; Atsushi Sakai; Hidenori Takahashi; Kyoko Toda; Ryuuichi Itoh

    Elsevier, 1998, International Review of Cytology, 1 - 41, doi;web_of_science

    In book

  • DNA synthesis in isolated mitochondrial nucleoids from plasmodia of Physarum polycephalum

    N Sasaki; A Sakai; S Kawano; H Kuroiwa; T Kuroiwa

    A mitochondrion contains multiple copies of mitochondrial DNA (mtDNA) in the mitochondrial nucleoid (mt-nucleoid, synonym for mitochondrial nuclei). Replicaton of mtDNA in the mt-nucleoids appears to be regulated within groups of adjacent mtDNA molecules, known as mitochondrial replicon clusters (MRCs). In this study, we isolated structurally intact mt-nucleoids from the plasmodia of Physarum polycephalum and characterized DNA synthesis in the isolated mt-nucleoids. The mt-nucleoids were isolated by dissolving the membranes of highly purified mitochondria with 0.5% Nonidet P-40. The structural integrity of the isolated mt-nucleoid was determined by observing the rod shape of the mt-nucleoid and the structure of the MRC. The isolated mt-nucleoids required four deoxyribonucleoside triphosphates and MgCl2 for DNA synthesis. The DNA synthesis was resistant to aphidicolin and showed only low sensitivity to N-ethylmaleimide and to ddTTP, suggesting that the DNA synthesis is catalyzed by plant-type mitochondrial DNA polymerase. The capacity for DNA synthesis in the isolated mt-nucleoids was similar to that in the isolated mitochondria, despite removal of most of the mitochondrial matrix and membrane. Furthermore, visualization of sites of DNA synthesis in vitro revealed that DNA synthesis in the isolated mt-nucleoids occurred in each MRC. These results suggest that the isolated mt-nucleoids are capable of efficient and systematic DNA synthesis in vitro. Therefore, the use of isolated mt-nucleoids should permit in vitro characterization of the molecular mechanism of mtDNA replication in the MRC., SPRINGER-VERLAG WIEN, 1998, PROTOPLASMA, 203 (3-4), 221 - 231, web_of_science

    Scientific journal

  • A High Density of rRNA in the Generative Cells and Sperm Cells of Pollen Grains of Five Angiosperm Species.

    Chieko Saito; Makoto Fujie; Atsushi Sakai; Noriko Nagata; Sachihiro Matsunaga; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    International Society of Cytology, Sep. 1998, CYTOLOGIA, 63 (3), 293 - 300, doi

    Scientific journal

  • 1,8-Cineole inhibits root growth and DNA synthesis in the root apical meristem ofBrassica campestris L.

    Ritsuko Koitabashi; Takeshi Suzuki; Tamotsu Kawazu; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, Mar. 1997, Journal of Plant Research, 110 (1), 1 - 6, doi;web_of_science;url;url;url

    Scientific journal

  • Preferential degradation of plastid DNA with preservation of mitochondrial DNA in the sperm cells of Pelargonium zonale during pollen development

    N Nagata; Sodmergen; C Saito; A Sakai; H Kuroiwa; T Kuroiwa

    In the present study, we studied changes in organellar DNA in the sperm cells of maturing pollen of Pelargonium zonale, a plant typical to exhibit biparental inheritance, by fluorescence microscopy after staining with 4',6-diamidino-2-phenylindole (DAPI) and by immunogold electron microscopy using anti-DNA antibody. Fluorescence intensities of DAPI-stained plastid nuclei in generative and sperm cells at various developmental stages were quantified with a video-intensified microscope photon counting system (VIMPCS). Results indicated that the amount of DNA per plastid in generative cells increased gradually during pollen development and reached a maximum value (about 70 T per plastid; 1 T represents the amount of DNA in a particle of T4 phage) in young sperm cells at 5 days before flowering. However, the DNA content of plastids was subsequently reduced to about 20% of the maximum value on the day of flowering. Moreover, the DNA content of the plastid further decreased to 4% of the maximum value when pollen grains were cultured for 6 h in germination medium. In contrast, the amount of DNA per mitochondrion did not decrease significantly around the flowering day. Similar results were also obtained by immunogold electron microscopy using anti-DNA antibody. The density of gold particles on plastids decreased during pollen maturation whereas labelling density on mitochondria remained relatively constant. The number of plastids and mitochondria per generative cell or per pair of sperm cells did not change significantly, indicating that the segregation of DNA by plastid division was not responsible for the decrease in the amount of DNA per plastid. These results indicate that the plastid DNA is preferentially degraded, but the mitochondrial DNA is preserved, in the sperm cells of P. zonale. While the plastid DNA of the sperm cells decreased before fertilization, it was also suggested that the low DNA contents that remain in the plastids of the sperm cells are enough to account for the biparental inheritance of prastids in P. zonale., SPRINGER WIEN, 1997, PROTOPLASMA, 197 (3-4), 217 - 229, web_of_science

    Scientific journal

  • Isolation of organellar DNA from Codium fragile (Codiaceae, Codiales, Ulvophyceae)

    Masaya Satoh; Atsushi Sakai; Shizue Hamazaki; Yuki Takashima; Tsuneyoshi Kuroiwa

    Wiley, Dec. 1997, Phycological Research, 45 (4), 213 - 216, doi;url

    Scientific journal

  • Amyloplast Formation in Cultured-Tobacco Cells II: Effects of Transcription/Translation Inhibitors on Accumulation of Starch.

    Atsushi Sakai; Yutaka Miyazawa; Kumiko Yashiro; Takeshi Suzuki; Shigeyuki Kawano

    International Society of Cytology, Sep. 1997, CYTOLOGIA, 62 (3), 295 - 301, doi

    Scientific journal

  • Variability of mitochondrial subgenomic molecules in the meristematic cells of higher plants.

    Takeshi Suzuki; Shigeyuki Kawano; Atsushi Sakai; Atsushi Hirai; Tsuneyoshi Kuroiwa

    Genetics Society of Japan, 1996, Genes & Genetic Systems, 71 (5), 329 - 333, doi;web_of_science;url

    Scientific journal

  • Isolation and developmental expression of male reproductive organ-specific genes in a dioecious campion, Melandrium album (Silene latifolia)

    Sachihiro Matsunaga; Shigeyuki Kawano; Hiroyoshi Takano; Hidenobu Uchida; Atsushi Sakai; Tsuneyoshi Kuroiwa

    Wiley, Oct. 1996, The Plant Journal, 10 (4), 679 - 689, doi;web_of_science;url

    Scientific journal

  • Amyloplast formation in cultured tobacco cells; effects of plant hormones on multiplication, size, and starch content

    Atsushi Sakai; Kumiko Yashiro; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Springer Science and Business Media LLC, Apr. 1996, Plant Cell Reports, 15 (8), 601 - 605, doi;web_of_science;url;url;url

    Scientific journal

  • Organelle DNA Synthesis before Cell Nuclear Replication is Essential for Subsequent Cell Propagation.

    Takeshi Suzuki; Atsushi Sakai; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    International Society of Cytology, Jun. 1996, CYTOLOGIA, 61 (2), 235 - 245, doi

    Scientific journal

  • Cytological Analysis of the Mature Pollen of Actinidia deliciosa (Kiwifruit).

    Sachihiro Matsunaga; Atsushi Sakai; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    International Society of Cytology, Sep. 1996, CYTOLOGIA, 61 (3), 337 - 341, doi

    Scientific journal

  • Localization of organelle DNA synthesis within the root apical meristem of rice

    Takeshi Suzuki; Narie Sasaki; Atsushi Sakai; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Oxford University Press (OUP), Jan. 1995, Journal of Experimental Botany, 46 (1), 19 - 25, doi;web_of_science;url

    Scientific journal

  • Preferential mitochondrial and plastid DNA synthesis before multiple cell divisions in Nicotiana tabacum.

    T. Suzuki; S. Kawano; A. Sakai; M. Fujie; H. Kuroiwa; H. Nakamura; T. Kuroiwa

    Organelle DNA synthesis in root meristem and cultured cell line BY-2, both derived from Nicotiana tabacum cv. Bright Yellow 2, was examined by immunofluorescence microscopy of Technovit sections with antibody against 5-bromodeoxyuridine (BrdU) and co-fluorescent staining with 4',6-diamidino-2-phenylindole (DAPI) and quantitative Southern hybridization. In the root meristem, the mitochondrial DNAs (mtDNAs) were synthesized in a specific region near to the quiescent center, where a low frequency of DNA synthesis of cell nuclei was observed. The mitochondrial nuclei (nucleoids) changed morphologically from long ellipsoids with a high frequency of DNA synthesis, in the region just above the quiescent center, to granules with a low frequency of DNA synthesis, as cell distance from the quiescent center increased. Similar patterns were observed in the cultured tobacco cell line (BY-2), in which large amounts of preferential synthesis of DNA of both mitochondria and plastids occurred prior to cell nuclear DNA synthesis just after stationary phase cells were transferred to fresh medium. Granular mitochondria which vigorously synthesized mtDNA were observed in both lag phase and logarithmic growth phase cells. However, long, ellipsoidal mitochondria which showed a low frequency of mtDNA synthesis were observed in stationary phase cells. Morphological changes of plastids were more conspicuous than those of mitochondria. After the medium was renewed, spherical plastids became extremely elongated and string-like, for 24 h, but were divided into small pieces after the third day. Vigorous synthesis of plastid DNA (ptDNA) occurred during this period of plastids elongation., COMPANY OF BIOLOGISTS LTD, Nov. 1992, JOURNAL OF CELL SCIENCE, 103, 831 - 837, web_of_science

    Scientific journal

  • Conversion of Proplastids to Amyloplasts in Tobacco Cultured Cells Is Accompanied by Changes in the Transcriptional Activities of Plastid Genes

    Atsushi Sakai; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Oxford University Press (OUP), 01 Oct. 1992, Plant Physiology, 100 (2), 1062 - 1066, doi;web_of_science;url

    Scientific journal

  • Transcriptional Activity of Morphologically Intact Proplastid-Nuclei (Nucleoids) Isolated from Tobacco Cultured Cells

    Atsushi Sakai; Hirofumi Yamashita; Yasuyuki Nemoto; Shigeyuki Kawano; Tsuneyoshi Kuroiwa

    Oxford University Press (OUP), Sep. 1991, Plant and Cell Physiology, 32, 835 - 843, doi;web_of_science;url

    Scientific journal

  • Dispersion of the structural organization of proplastid-nuclei in vitro changes the transcriptional activity of plastid genes.(共著)

    A SAKAI; S KAWANO; T KUROIWA

    We have developed a novel assay system for analyzing the relationship between the structure and the transcriptional activity of the plastid-nuclei (plastid-nucleoids). The organization of morphologically intact proplastid-nuclei, isolated from tobacco cultured cells (line BY-2), was dispersed by treatment with NaCl at various concentrations and their transcriptional activities were examined by an assay of transcription in vitro and Southern hybridization. Disturbance of the structural organization of the proplastid-nuclei caused changes in both absolute and relative transcriptional activities of plastid genes, a result that suggests that the transcriptional activity of plastid genes may actually be regulated by structural changes in the plastid-nuclei., SPRINGER-VERLAG WIEN, Jun. 1991, PROTOPLASMA, 163 (2-3), 203 - 206, web_of_science

    Scientific journal

  • RAPD isolation of a Y chromosome specific ORF in a dioecious plant, Silene latifolia.

    Nakao S; Matsunaga S; Sakai A; Kuroiwa T; Kawano S

    Apr. 2002, Genome, 45, 413 - 420

  • Senescence in the nongreening region of the rice (Oryza sativa) coleoptile

    Inada N; Sakai A; KuroiwaH; Kuroiwa T

    2000, Protoplasma, 214, 180 - 193

    Scientific journal

  • Changes in the Extent of the Condensation of Nuclear Chromatin and the Localization of RNA During Pollen Development in Nicotiana tabacum

    Chieko Saito; Makoto Fujie; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa

    International Society of Cytology, Jun. 1997, CYTOLOGIA, 62 (2), 121 - 132, doi

    Scientific journal

MISC

  • プラスチド核(核様体)とDNA合成

    SAKAI Atsushi

    ミニレビュー, 2008, Plant Organelles News Letter, 7

  • 色素体の細胞科学-人類存続の科学へ-(共著)

    SAKAI Atsushi

    1996, 生物科学の現状と展望(「遺伝」別冊), 22-35

  • New Images on the Proliferation of Plant Cells ; Regulation by organelles.

    SAKAI Atsushi

    1995, Research Journal of Food and Agriculture, 18 (4), 18-22

  • Function and Differentiation of Plastids.

    SAKAI Atsushi

    1995, 「細胞工学」別冊 植物細胞工学シリーズ3 「植物の分子細胞生物学」, 110-123

  • Plastid Differentiation from the viewpoint of Plastid-nuclei

    SAKAI Atsushi

    1993, Plant Cell Technology, 5 (5), 366

  • Plant cells and genes.

    SAKAI Atsushi

    1992, Metals and Technology "KINZOKU", 62, 8

  • 葉緑体の遺伝子発現-植物はどのようにして色素体を作動させるか-(共著翻訳)

    SAKAI Atsushi

    1990, 分子・細胞レベルからみた高等植物(「植物細胞工学」2巻臨時増刊号), 2 (臨時増刊), 321-335

  • Generative Cells

    Atsushi Sakai

    Springer Japan, 2014, Atlas of Plant Cell Structure, 157 - 186, doi;url

    Introduction other

Books etc

  • 植物学の百科事典

    SAKAI Atsushi (, Range: 分担)

    丸善出版株式会社, 2016

  • 生物学辞典

    SAKAI Atsushi (, Range: 分担)

    東京化学同人, Dec. 2010

  • 「大台ケ原の自然誌―森の中のシカをめぐる生物間相互作用」

    SAKAI Atsushi (, Range: 分担)

    東海大学出版会, 2009, 第11章 シカの影響の有無によるササの形態と光合成能力の変化

  • 生物学大辞典

    SAKAI Atsushi (, Range: 分担)

    東京化学同人, 2007

  • プラントミメティックスー−植物に学ぶー−

    SAKAI Atsushi (, Range: 分担)

    エヌ・ティー・エス, 2006, pp. 279 - 285, 葉緑体と色素体-色素体七変化-

  • 光合成事典

    SAKAI Atsushi (, Range: 分担)

    学会出版センター, 2003

  • 細胞生物学事典

    SAKAI Atsushi (, Range: 分担)

    朝倉書店, 2002

  • 分子生物学(共著)

    SAKAI Atsushi

    東京化学同人, 1999, 27-35頁

  • 分子細胞生物学辞典

    SAKAI Atsushi (, Range: 分担)

    東京化学同人, 1997

  • オルガネラ形成の遺伝子発現機構(共著)

    SAKAI Atsushi

    講談社サイエンティフィク,「植物の遺伝子発現」, 1994, 214-231頁

Presentations

  • マングローブ植物ヤエヤマヒルギのイオン吸収速度に対するNa+の影響 ~modified root-split法を用いた解析~

    SAKAI Atsushi

    日本植物形態学会第30回大会, Sep. 2018, 広島

  • トウモロコシ第一葉老化過程における葉緑体核様体の挙動と光合成特性の変化

    SAKAI Atsushi

    日本植物形態学会第30回大会, Sep. 2018, 広島

  • タバコ培養細胞BY-2の細胞死誘導過程におけるDNA断片化へのタンパク質合成阻害の影響

    SAKAI Atsushi

    日本植物形態学会第29回大会, Sep. 2017, 日本植物形態学会, 千葉県野田市

  • 地下部からの滲出による成長阻害物質の放出を検出する実験法の検討

    SAKAI Atsushi

    日本植物学会第81回大会, Sep. 2017, 日本植物学会, 千葉県野田市

  • セイタカアワダチソウの生葉・枯葉由来成長阻害物質の同定に向けた解析

    SAKAI Atsushi

    日本植物形態学会第28回大会, Sep. 2016, 日本植物形態学会, 沖縄

  • タバコ培養細胞BY-2の3種類の細胞死誘導過程におけるDNA断片化プロセスの検討

    SAKAI Atsushi

    日本植物形態学会第28回大会, Sep. 2016, 日本植物形態学会, 沖縄

  • ヒマラヤスギの根からの成長阻害物質放出について

    SAKAI Atsushi

    日本植物形態学会第28回大会, Sep. 2016, 日本植物形態学会, 沖縄

  • ヒマラヤスギ周囲の裸地形成における枯葉の役割

    SAKAI Atsushi

    日本植物形態学会第27回大会, Sep. 2015, 新潟

  • 単細胞緑藻Chlamydomonas reinhardtii 高温耐性株の解析

    SAKAI Atsushi

    日本植物形態学会第27回大会, Sep. 2015, 新潟

  • セイタカアワダチソウの枯葉由来成長阻害物質の同定に向けて

    SAKAI Atsushi

    日本植物形態学会第27回大会, Sep. 2015

  • 低温下における葉の赤色化は葉温を上昇させるか?

    SAKAI Atsushi

    日本植物形態学会第27回大会, Sep. 2015, 新潟

  • タバコ培養細胞BY-2の細胞密度が過敏感細胞死誘導に及ぼす影響 ―過敏感細胞から発信されるシグナル物質の関与の可能性―

    SAKAI Atsushi

    日本植物形態学会第27回大会, Sep. 2015

  • ヒマラヤスギ周囲の裸地形成には複数の要因が関与する

    SAKAI Atsushi

    日本植物形態学会第26回大会, Sep. 2014, 日本植物形態学, 神奈川県横浜市生田

  • 接触形態形成が窒素含量の変化を通して光合成に及ぼす影響

    SAKAI Atsushi

    日本植物形態学会第26回大会, Sep. 2014, 日本植物形態学会, 神奈川県横浜市生田

  • タバコ培養細胞BY-2の細胞学:細胞の増殖・分化と死

    SAKAI Atsushi

    日本植物学会第78回大会, Sep. 2014, 日本植物学会、日本メンデル協会, 神奈川県横浜市生田

  • 接触形態形成と光合成の接点

    SAKAI Atsushi

    近畿植物学会講演会, Dec. 2013, 奈良

  • 栄養環境の違いが接触形態形成、窒素含量、光合成能力に及ぼす影響

    SAKAI Atsushi

    日本植物形態学会第25回大会, Sep. 2013, 札幌

  • 機械的ストレスに対するシロイヌナズナの応答:サイズ、窒素含量、および光合成機能の変化

    SAKAI Atsushi

    日本植物学会第77回大会, Sep. 2013, 札幌

  • 盗葉緑体が光合成ウミウシの走光性に与える影響

    SAKAI Atsushi

    第60回日本生態学会大会, Mar. 2013, 静岡

  • ヒマラヤスギ周囲の裸地は他感作用によるものか?II. 他感物質の供給経路と季節変化

    SAKAI Atsushi

    日本植物形態学会第24回大会, Sep. 2012, 東京

  • ヤエヤマヒルギ幼植物体の生存・成長・呼吸・光合成に対するNaClの影響

    SAKAI Atsushi

    日本植物形態学会第24回大会, Sep. 2012, 東京

  • オルガネラ核様体におけるMicrococcal nucleaseによる分解からDNAを保護する構造の比較

    SAKAI Atsushi

    日本植物形態学会第24回大会, Sep. 2012, 東京

  • 接触形態形成が窒素含量の変化を介して光合成能力に影響を及ぼす現象について:刺激耐性の異なる種間での比較

    SAKAI Atsushi

    日本植物学会第76回大会, Sep. 2012, 姫路

  • ヒマラヤスギ周囲の裸地は他感作用によるものか?

    SAKAI Atsushi

    日本植物形態学会第23回大会, Sep. 2011, 東京

  • 接触形態形成による植物体サイズの変化は植物体内窒素濃度の変化を介して光合成能力の変化をもたらす

    SAKAI Atsushi

    日本植物学会第75回大会, Sep. 2011, 東京

  • タバコ培養細胞におけるPCDと非PCDに伴う核DNAの断片化

    SAKAI Atsushi

    日本植物学会第75回大会, Sep. 2011

  • Reevaluation of allelopathic potential of goldenrod (Solidago altissima) naturalized in Japan.

    Atsushi Sakai; Yukiko Ushikoshi; Akiko Sasaki

    第61回日本植物生理学会年会, 21 Mar. 2020, 19 Mar. 2020, 21 Mar. 2020

Association Memberships

  • 日本植物学会

  • 日本植物生理学会

  • 日本植物形態学会



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