Researchers Database

YOSHIKAWA Hisao

    Faculty Division of Natural Sciences Research Group of Biological Sciences Associate Professor
Last Updated :2021/07/07

researchmap

Degree

  • Doctor of Medical Science, Kyoto Prefectural University of Medicine
  • Master of Science, Konan University

Research Interests

  • protist, diversity, molecular phylogeny, Blastocystis 

Research Areas

  • Life sciences, Parasitology
  • Life sciences, Biodiversity and systematics
  • Life sciences, Cell biology

Research Experience

  • Apr. 2014 Mar. - 2015, 金沢大学 非常勤講師
  • May 2013 May - 2013, 京都府立医科大学・非常勤講師
  • Apr. 2012 Mar. - 2013, 京都府立医科大学 非常勤講師
  • Apr. 2001 Mar. - 2009, Gifu University, School of Medicine, \npart-time instructor
  • Apr. 1990 Mar. - 2009, Kyoto Prefectural University of Medicine\npart-time instructor
  • Apr. 2007, 奈良女子大学 准教授
  • Sep. 1990 Aug. - 1991, University of Oklahoma, Department of Microbiology and Immunology, Health Sciences Center, Visiting Professor
  • Sep. 1989 Mar. - 1990, 京都大学医療技術短期大学部\n非常勤講師
  • Nov. 1989, Nara Women's University\nAssociate Professor
  • Apr. 1986 Oct. - 1989, Kyoto Prefectural University of Medicine\nResearch Associate
  • Apr. 1988 Mar. - 1989, Kyoto Women's University\npart-time instructor

Education

  • - 1986, Kyoto Prefectural University of Medicine, Graduate School, Division of Medicine, 病理系
  • - 1982, Konan University, Graduate School, Division of Science and Technology (Konan University), Biology
  • - 1980, Konan University, Faculty of Science, 生物学

Committee Memberships

  • Jan. 2015Parasitology ResearchReviewer
  • Apr. 2004日独原虫病協会評議委員、監事
  • 1999日本寄生虫学会評議委員
  • Apr. 1997 Mar.2019日本顕微鏡学会関西支部評議員

Awards

  • The third prize for poster presentation, Hisao Yoshikawa, Turkish Microbiology Committee, Nov. 2010
  • 風戸研究奨励賞, 財団法人風戸研究奨励会, 1992

Published Papers

  • Three monophyletic clusters in Retortamonas species isolated from vertebrates.

    Joko Hendarto; Tetsushi Mizuno; Anggi P N Hidayati; Ismail E Rozi; Puji B S Asih; Din Syafruddin; Hisao Yoshikawa; Makoto Matsubayashi; Masaharu Tokoro

    Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet been elucidated. Haplotypes of the 18S small subunit ribosomal RNA locus (1836-1899 bp) of Retortamonas spp. from humans (n = 8), pigs (n = 6), dogs (n = 1), goats (n = 16), water buffalos (n = 23), cattle (n = 7), rats (n = 3), and chickens (n = 5) were analyzed with references isolated from non-human mammals, amphibians, and insects. Phylogenetic and network analyses revealed a statistically supported three cluster formation among the vertebrate-isolated haplotypes, while insect-isolated haplotypes were independently clustered with Chilomastix. In the clade of vertebrate isolates, assemblage A (amphibian genotype), which included the amphibian references, was addressed as an out-group of the other clusters. Assemblage B (mammalian and chicken genotype) included most haplotypes from various mammals including humans with the haplotypes isolated from a chicken. Human isolates were all classified into this assemblage, thus assemblage B might correspond to R. intestinalis. Assemblage C (bovine genotype), which included specific haplotypes from water buffalos and cattle, was addressed as a sister lineage of assemblage B. Among the diversified haplotypes of assemblage B, a specific haplotype, which was identified from multiple host mammals (humans, dogs, pigs, cattle, water buffalos, elks, goats, and rats), indicates the potential zoonotic transmission of the Retortamonas among them. The genotyping classification of retortamonads could contribute to a better understanding of its molecular epidemiology, especially among humans and related host organisms., Apr. 2019, Parasitology international, 69 (1), 93 - 98, True, doi;pubmed

  • Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia.

    Takahiro Matsumura; Joko Hendarto; Tetsushi Mizuno; Din Syafruddin; Hisao Yoshikawa; Makoto Matsubayashi; Taro Nishimura; Masaharu Tokoro

    Background: Although parasites are still endemic in developing areas, residents in those regions seem not to be affected by the presence of intestinal protozoans. This study aimed to investigate whether pathogenic and commensal protozoans are the causal agents of diarrhea via a school-based cross-sectional survey conducted in Indonesia, in September 2016. Results: Molecular screening for intestinal protozoans in collected 144 stool samples from healthy students (age range 7-15 years) was carried out. The prevalence of protozoan parasites was as follows: Giardia intestinalis (56.3%), Entamoeba histolytica (0%), E. dispar (6.9%), E. moshkovskii (0%), E. hartmanni (31.3%), and E. coli (44.4%). Observational evaluation of stool conditions using the Bristol stool chart confirmed the loose stool rate (33.3-90.9%) in each age group. Logistic regression analysis of protozoan infection or colonization for loose stool (mild to severe diarrhea) as an outcome revealed no significant findings in examined protozoans including pathogenic G. intestinalis infection [adjusted odds ratio (AOR) 0.78, 95% confidence interval (CI) 0.36-1.67], except in E. hartmanni colonization (AOR 2.81, 95% CI 1.1-3.7, P = 0.026). Conclusions: The molecular survey of intestinal protozoans targeting healthy population with their stool form evaluation could address the pathogenicity of those parasites appropriately. In comparatively higher-age children at least 7 years of age or greater in the endemic area, G. intestinalis could regard commensal, while E. hartmanni seems to possess a certain pathogenicity as a causal agent of mild diarrhea., 2019, Tropical medicine and health, 47 (7), 7 - 7, False, doi;pubmed;pmc

  • Molecular phylogeny of Blastocystis isolates from wild rodents captured in Indonesia and Japan

    Maai Katsumata; Hisao Yoshikawa; Masaharu Tokoro; Tetsushi Mizuno; Takehiro Nagamoto; Joko Hendarto; Puji B.S. Asih; Ismail E. Rozi; Isao Kimata; Kazutoshi Takami; Din Syafruddin

    Springer Science and Business Media LLC, Sep. 2018, Parasitology Research, 117 (9), 2841 - 2846, doi;url;url;rm:research_project_id

    Scientific journal

  • Human Blastocystis subtyping with subtype-specific primers developed from unique sequences of the SSU rRNA gene

    Hisao Yoshikawa; Ayana Iwamasa

    Elsevier BV, Dec. 2016, Parasitology International, 65 (6), 785 - 791, doi;web_of_science

    Scientific journal

  • Molecular survey of Blastocystis sp. from humans and associated animals in an Indonesian community with poor hygiene

    Hisao Yoshikawa; Masaharu Tokoro; Takehiro Nagamoto; Shunsuke Arayama; Puji B.S. Asih; Ismail E. Rozi; Din Syafruddin

    Elsevier BV, Dec. 2016, Parasitology International, 65 (6), 780 - 784, doi;web_of_science;rm:research_project_id

    Scientific journal

  • Blastocystis phylogeny among various isolates from humans to insects

    Hisao Yoshikawa; Yukiko Koyama; Erika Tsuchiya; Kazutoshi Takami

    Blastocystis is a common unicellular eukaryotic parasite found not only in humans, but also in various kinds of animal species worldwide. Since Blastocystis isolates are morphologically indistinguishable, many molecular biological approaches have been applied to classify these isolates. The complete or partial sequences of the small subunit rRNA gene (SSU rDNA) are mainly used for comparisons and phylogenetic analyses among Blastocystis isolates. However, various lengths of the partial SSU rDNA sequence have been used for phylogenetic inference among genetically different isolates. Based on the complete SSU rDNA sequences, consensus terminology of nine subtypes (STs) of Blastocystis sp. that were supported by phylogenetically monophyletic nine clades was proposed in 2007. Thereafter, eight additional kinds of STs comprising non-human mammalian Blastocystis isolates have been reported based on the phylogeny of SSU rDNA sequences, while STs 11 and 12 were only proposed on the base of partial sequences. Although many sequence data from mammalian and avian Blastocystis are registered in GenBank, only limited data on SSU rDNA are available for poikilotherm-derived Blastocystis isolates. Therefore, the phylogenetic positions of the reptilian/amphibian Blastocystis clades are unstable. The phylogenetic inference of various STs comprising mammalian and/or avian Blastocystis isolates was verified herein based on comparisons between partial and complete SSU rDNA sequences, and the phylogenetic positions of reptilian and amphibian Blastocystis isolates were also investigated using 14 new Blastocystis isolates from reptiles with all known isolates from other reptilians, amphibians, and insects registered in GenBank. (C) 2016 Published by Elsevier Ireland Ltd., ELSEVIER IRELAND LTD, Dec. 2016, PARASITOLOGY INTERNATIONAL, 65 (6), 750 - 759, doi;web_of_science

    Scientific journal

  • Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia

    Mudyawati Kamaruddin; Masaharu Tokoro; Md. Moshiur Rahman; Shunsuke Arayama; Anggi P. N. Hidayati; Din Syafruddin; Puji B. S. Asih; Hisao Yoshikawa; Ei Kawahara

    Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mills, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species., KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI, Oct. 2014, KOREAN JOURNAL OF PARASITOLOGY, 52 (5), 471 - 478, doi;web_of_science

    Scientific journal

  • Genetic Diversity of Blastocystis in Livestock and Zoo Animals

    Mohammed A. Alfellani; Derya Taner-Mulla; Alison S. Jacob; Christine Atim Imeede; Hisao Yoshikawa; C. Rune Stensvold; C. Graham Clark

    Blastocystis is a common unicellular anaerobic eukaryote that inhabits the large intestine of many animals worldwide, including humans. The finding of Blastocystis in faeces in mammals and birds has led to proposals of zoonotic potential and that these hosts may be the source of many human infections. Blastocystis is, however, a genetically diverse complex of many distinct organisms (termed subtypes; STs), and sampling to date has been limited, both geographically and in the range of hosts studied. In order to expand our understanding of host specificity of Blastocystis STs, 557 samples were examined from various non-primate animal hosts and from a variety of different countries in Africa, Asia and Europe. STs were identified using 'barcoding' of the small subunit rRNA gene using DNA extracted either from culture or directly from faeces. The host and geographic range of several STs has thereby been greatly expanded and the evidence suggests that livestock is not a major contributor to human infection. Two new STs were detected among the barcode sequences obtained; for these, and for three others where the data were incomplete, the corresponding genes were fully sequenced and phylogenetic analysis was undertaken. (c) 2013 Elsevier GmbH. All rights reserved., ELSEVIER GMBH, URBAN & FISCHER VERLAG, Jul. 2013, PROTIST, 164 (4), 497 - 509, doi;web_of_science

    Scientific journal

  • Subtype identification of Blastocystis spp. isolated from patients in a major hospital in northeastern Thailand

    Sarinee Jantermtor; Porntip Pinlaor; Kookwan Sawadpanich; Somchai Pinlaor; Arunnee Sangka; Chotechana Wilailuckana; Wachanan Wongsena; Hisao Yoshikawa

    The present study is aimed to identify the prevalence of Blastocystis subtypes isolated from patients in a major hospital in northeastern Thailand. A total of 562 stool samples were examined by culture technique, and 56 Blastocystis-positive samples were analyzed further by the combination of restriction fragment length polymorphism (RFLP) followed by polymerase chain reaction with sequence-tagged site primers (PCR-STS). By RFLP profiles, Blastocystis genotypes were categorized into four groups: group A (12, 21.4 %), group B (32, 57.1 %), group C (10, 17.9 %), and group D (2, 3.6 %). By PCR-STS, only four subtypes were identified. All 12 (21.4 %) isolates in group A were identified as subtype 1. Similarly, all 32 (57.1 %) isolates in group B were subtype 3. In group C, 10 (17.9 %) samples were all subtype 7, and two samples (3.6 %) in group D were both subtype 6. Of 56 Blastocystis-positive patients, 31 (55.4 %) were asymptomatic and 22 (39.4 %) have gastrointestinal symptoms. No significant association was observed between the Blastocystis subtypes and the clinical features. Among the Blastocystis-positive patients, the most characteristic stool samples were loose (78.6 %) and soft (17.9 %). In conclusion, the most common Blastocystis spp. in northeastern Thailand was subtype 3 followed by subtype 1. Relatively minor subtypes, subtype 6 and subtype 7 which are considered as avian subtypes, were found for the first time in humans in Thailand. © 2012 Springer-Verlag Berlin Heidelberg., Apr. 2013, Parasitology Research, 112 (4), 1781 - 1786, doi;pubmed

    Scientific journal

  • Morphology of human and animal Blastocystis isolates with special reference to reproductive modes.

    YOSHIKAWA Hisao; Minoru Yamamada; Hisao Yoshikawa

    Dec. 2012, Blastocystis:Pathogen or Passenger?, 9-35

  • Epidemiology, transmission, and zoonotic potential of Blastocystis in human and animals

    YOSHIKAWA Hisao

    Dec. 2012, Blastocystis:Pathogen or Passenger?, 37-49

  • Molecular approaches on systematic position, genetic polymorphism, and classification of Blastocystis from humans and animals.

    YOSHIKAWA Hisao

    Dec. 2012, Blastocystis:Pathogen or Passenger?, 85-94

  • Landmarks in the Discovery of Blastocystis Stages

    YOSHIKAWA Hisao; H. Mehlhorn; M. Yamada; H. Yoshikawa

    Dec. 2012, Blastocystis: Pathogen or Passenger?, 1-8

  • A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia

    M. Tokoro; H. Yoshikawa; D. Syafruddin

    ELSEVIER SCI LTD, Jun. 2012, INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 16, E177 - E177, doi;web_of_science

  • 赤痢アメーバ感染症の2例:Entamoeba disper感染にヒトブラストシスチスおよび小型アメーバが検出されたブラジル人症例と回腸部に潰瘍を認めた無症候性E. histolytica感染の日本人症例

    YOSHIKAWA Hisao

    2012, Clinical Parasitology, 23 (1), 82-85

  • Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples

    Hisao Yoshikawa; Funda Dogruman-AI; Songul Turk; Semra Kustimur; Neriman Balaban; Nedim Sultan

    Springer Science and Business Media LLC, Oct. 2011, Parasitology Research, 109 (4), 1045 - 1050, doi;web_of_science;url;url;url

    Scientific journal

  • PCR-based subtyping of Blastocystis isolates from symptomatic and asymptomatic individuals in a major hospital in Ankara, Turkey

    Funda Dogruman-Al; Hisao Yoshikawa; Semra Kustimur; Neriman Balaban

    Springer Science and Business Media LLC, Dec. 2009, Parasitology Research, 106 (1), 263 - 268, doi;url;url;url

    Scientific journal

  • Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey

    Funda Dogruman-Al; Semra Kustimur; Hisao Yoshikawa; Candan Tuncer; Zahide Simsek; Mehmet Tanyuksel; Engin Araz; Kenneth Boorom

    Blastocystis infection has been reported to be associated with irritable bowel syndrome (IBS), inflammatory bowel disease (IBD) and chronic diarrhoea. The availability of data on the subtypes of Blastocystis found in these patient groups would be of interest in understanding the significance of Blastocystis infection in chronic illness. In this study, we identify Blastocystis subtypes found in patients presenting with IBS, IBD, chronic diarrhoea and asymptomatic patients in Ankara, Turkey. Blastocystis was detected in 11 symptomatic patients by microscopy and 19 by stool culture. Stool culture was more sensitive than microscopy in identifying Blastocystis. Using standard nomenclature adopted in 2007, Blastocystis sp. subtype 3 was the most common in all groups, followed by Blastocystis sp. subtype 2. Identical subtypes of Blastocystis are found in patients with IBS, IBD and chronic diarrhoea. These particular subtypes show low host specificity and are carried by humans and some farm animals. The subtypes of Blastocystis that are commonly found in rodents and certain wild birds were not found in these patients. We suggest a model in which the severity of enteric protozoan infection may be mediated by host factors., FUNDACO OSWALDO CRUZ, Aug. 2009, MEMORIAS DO INSTITUTO OSWALDO CRUZ, 104 (5), 724 - 727, web_of_science

    Scientific journal

  • Expression of interferon gamma and proinflammatory cytokines in the cecal mucosa of rats experimentally infected with Blastocystis sp. strain RN94-9

    Aya Iguchi; Hisao Yoshikawa; Minoru Yamada; Isao Kimata; Naoki Arizono

    Springer Science and Business Media LLC, Jun. 2009, Parasitology Research, 105 (1), 135 - 140, doi;url;url;url

    Scientific journal

  • Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal

    Hisao Yoshikawa; Zhiliang Wu; Kishor Pandey; Basu Dev Pandey; Jeevan Bahadur Sherchand; Tetsuo Yanagi; Hiroji Kanbara

    Elsevier BV, Mar. 2009, Veterinary Parasitology, 160 (3-4), 295 - 300, doi;web_of_science

    Scientific journal

  • A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis

    Funda Dogruman-Al; Hande Dagci; Hisao Yoshikawa; Özgur Kurt; Mete Demirel

    Springer Science and Business Media LLC, Aug. 2008, Parasitology Research, 103 (3), 685 - 689, doi;web_of_science;url;url;url

    Scientific journal

  • Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore

    Kenneth H. S. Wong; G. C. Ng; Raymond T. P. Lin; H. Yoshikawa; Mark B. Taylor; Kevin S. W. Tan

    Springer Science and Business Media LLC, Mar. 2008, Parasitology Research, 102 (4), 663 - 670, doi;web_of_science;url;url;url

    Scientific journal

  • Molecular epidemiology of human Blastocystis in a village in Yunnan province, China

    Lan-Hua Li; Xiao-Nong Zhou; Zun-Wei Du; Xue-Zhong Wang; Li-Bo Wang; Jin-Yong Jiang; Hisao Yoshikawa; Peter Steinmann; Jürg Utzinger; Zhiliang Wu; Jia-Xu Chen; Shao-Hong Chen; Ling Zhang

    Elsevier BV, Dec. 2007, Parasitology International, 56 (4), 281 - 286, doi;web_of_science

    Scientific journal

  • Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China

    Lan Hua Li; Xiao Ping Zhang; Shan Lv; Ling Zhang; Hisao Yoshikawa; Zhiliang Wu; Peter Steinmann; Jürg Utzinger; Xiao Mei Tong; Shao Hong Chen; Xiao Nong Zhou

    Springer Science and Business Media LLC, 18 Oct. 2007, Parasitology Research, 102 (1), 83 - 90, doi;url;url;url

    Scientific journal

  • Infectivity of different genotypes of human Blastocystis hominis isolates in chickens and rats

    Aya Iguchi; Akiko Ebisu; Satiko Nagata; Yumie Saitou; Hisao Yoshikawa; Satoru Iwatani; Isao Kimata

    Elsevier BV, Jun. 2007, Parasitology International, 56 (2), 107 - 112, doi;web_of_science

    Scientific journal

  • Terminology for Blastocystis subtypes – a consensus

    C. Rune Stensvold; G. Kumar Suresh; Kevin S.W. Tan; R.C. Andrew Thompson; Rebecca J. Traub; Eric Viscogliosi; Hisao Yoshikawa; C. Graham Clark

    Elsevier BV, Mar. 2007, Trends in Parasitology, 23 (3), 93 - 96, doi;web_of_science

    Scientific journal

  • Ultrastructural and Phylogenetic Studies on Blastocystis Isolates from Cockroaches

    HISAO YOSHIKAWA; ZHILIANG WU; JOSEPHINE HOWE; TETSUO HASHIMOTO; NG GEOK-CHOO; KEVIN S. W. TAN

    Wiley, Jan. 2007, The Journal of Eukaryotic Microbiology, 54 (1), 33 - 37, doi;web_of_science

    Scientific journal

  • Zoonotic potential and cyst passage of human Blastocystis hominis.

    YOSHIKAWA Hisao; Saitou Y

    Dec. 2006, Parasitic Zoonosis in Asian-Pacific Regions 2006, 20-24

  • Infectivity and pathogenicity of Blastocystis.

    YOSHIKAWA Hisao; Iguchi A

    Dec. 2006, Parasitic Zoonosis in Asian-Pacific Regions 2006, 25-31

  • アメーバ性大腸炎にBlastocystis hominisとGiardia lambliaの同時感染を認めた一症例

    YOSHIKAWA Hisao

    2006, 岐阜市民病院年報, 26, 47-51

  • Infectivity of Blastocystis isolates from chickens, quails and geese in chickens

    Aya Tanizaki; Hisao Yoshikawa; Satoru Iwatani; Isao Kimata

    Springer Science and Business Media LLC, Apr. 2005, Parasitology Research, 96 (1), 57 - 61, doi;url;url;url

    Scientific journal

  • Fecal-oral transmission of the cyst form of Blastocystis hominis in rats

    Hisao Yoshikawa; Kumi Yoshida; Ayumi Nakajima; Kimie Yamanari; Satoru Iwatani; Isao Kimata

    Springer Science and Business Media LLC, Dec. 2004, Parasitology Research, 94 (6), 391 - 396, doi;web_of_science;url;url;url

    Scientific journal

  • Problems in speciation in the genus Blastocystis

    Hisao Yoshikawa; Keiko Morimoto; Zhiliang Wu; Mulkit Singh; Tetsuo Hashimoto

    Elsevier BV, Jun. 2004, Trends in Parasitology, 20 (6), 251 - 255, doi;web_of_science

    Scientific journal

  • A survey of Blastocystis infection in anuran and urodele amphibians

    Hisao Yoshikawa; Keiko Morimoto; Minako Nagashima; Naoko Miyamoto

    Elsevier BV, Jun. 2004, Veterinary Parasitology, 122 (2), 91 - 102, doi;web_of_science

    Scientific journal

  • PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds

    Hisao Yoshikawa; Niichiro Abe; Zhiliang Wu

    Microbiology Society, 01 May 2004, Microbiology, 150 (5), 1147 - 1151, doi;web_of_science;url

    Scientific journal

  • クリプトスポリジウムの特異な細胞内寄生様式について

    YOSHIKAWA Hisao

    Mar. 2004, 顕微鏡, 39 (1), 48-52

  • Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries

    Hisao Yoshikawa; Zhiliang Wu; Isao Kimata; Motohiro Iseki; Ibne Karim; M D Ali; Momammad B Hossain; Viqar Zaman; Rashidul Haque; Yuzo Takahashi

    Springer Science and Business Media LLC, 01 Jan. 2004, Parasitology Research, 92 (1), 22 - 29, doi;web_of_science;url;url;url

    Scientific journal

  • Genomic Polymorphism Among Blastocystis Isolates and Development of PCR-Based Identification of Zoonotic Isolates

    HISAO YOSHIKAWA; NIICHIRO ABE; ZHILIANG WU

    Wiley, Jul. 2003, The Journal of Eukaryotic Microbiology, 50 (s1), 710 - 711, doi;web_of_science

    Scientific journal

  • Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene

    Niichiro Abe; Zhiliang Wu; Hisao Yoshikawa

    Springer Science and Business Media LLC, Jun. 2003, Parasitology Research, 90 (2), 124 - 128, doi;web_of_science;url;url

    Scientific journal

  • Molecular characterization of Blastocystis isolates from primates

    Niichiro Abe; Zhiliang Wu; Hisao Yoshikawa

    Elsevier BV, May 2003, Veterinary Parasitology, 113 (3-4), 321 - 325, doi

    Scientific journal

  • Molecular characterization of Blastocystis isolates from birds by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene

    Niichiro Abe; Zhiliang Wu; Hisao Yoshikawa

    Springer Science and Business Media LLC, Mar. 2003, Parasitology Research, 89 (5), 393 - 396, doi;web_of_science;url;url

    Scientific journal

  • Freeze-Fracture and Cytoehemical Studies on the in Vitro Cyst Form of Reptilian Blastocystis pythoni

    HISAO YOSHIKAWA; MINAKO NAGASHIMA; KEIKO MORIMOTO; YASUKO YAMANOUTI; EU HIAN YAP; MULKIT SINGH

    Wiley, Jan. 2003, The Journal of Eukaryotic Microbiology, 50 (1), 70 - 75, doi

    Scientific journal

  • Molecular comparative studies among Blastocystis isolates obtained from humans and animals

    Hisao Yoshikawa; Zhiliang Wu; Isao Nagano; Yuzo Takahashi

    This study compared specific polymerase chain reaction (PCR) primers and phylogenetic tree analysis of restriction fragment length polymorphism using the small subunit ribosomal RNA gene in various Blastocystis populations. A phylogenetic tree was constructed using 12 restriction enzymes and a sample pool of 22 isolates, including 2 reference strains and Proteromonas lacertae as an outgroup. The analysis showed that the 22 isolates could be separated into 7 clusters. Four of the 7 clusters were mixed groups that comprised isolates from both humans and nonhuman hosts. The other 3 clusters contained isolates from humans or nonhuman hosts only. The phylogenetic analysis also showed that B. hominis isolates from geographical separated areas did not necessarily cluster in the genetically different groups. The results of genetic homology and phylogenetic tree analysis among Blastocystis isolates from humans and animals indicated that all isolates from animals appear to be B. hominis. Polymerase chain reaction amplifications using previously described and newly defined specific primers mirrored the clusters obtained by the phylogenetic tree analysis. Our results show that primer PCR can be used as a powerful tool for the typing of Blastocystis populations., American Society of Parasitologists, 2003, Journal of Parasitology, 89 (3), 585 - 594, doi;pubmed

    Scientific journal

  • Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates

    N. ARISUE; T. HASHIMOTO; H. YOSHIKAWA

    Genes encoding small subunit ribosomal RNA (SSUrRNA) of 16 Blastocystis isolates from humans and other animals were amplified by the polymerase chain reaction, and the corresponding fragments were cloned and sequenced. Alignment of these sequences with the previously reported ones indicated the presence of 7 different sequence patterns in the highly variable regions of the small subunit ribosomal RNA. Phylogenetic reconstruction analysis using Proteromonas lacertae as the outgroup clearly demonstrated that the 7 groups with the different sequence patterns are separated to form independent clades, 5 of which consisted of the Blastocystis isolates from both humans (B. hominis) and other animals. The presence of 3 higher order clades was also clearly supported in the phylogenetic tree. However, a relationship among the 4 groups including these 3 higher order clades was not settled with statistical confidence. The remarkable heterogeneity of small subunit ribosomal RNAs among different Blastocystis isolates found in this study confirmed, with sequence-based evidence, that these organisms are genetically highly divergent in spite of their morphological identity. The highly variable small subunit ribosomal RNA regions among the distinct groups will provide useful information for the development of group-specific diagnostic primers., Cambridge University Press (CUP), Jan. 2003, Parasitology, 126 (1), 1 - 9, doi;web_of_science;url

    Scientific journal

  • Formation of palladium(0) nanoparticles from a microemulsion system composed of bis (N-octylethylenediamine)palladium(II) chloride complex

    M Iida; S Ohkawa; H Er; N Asaoka; H Yoshikawa

    Palladium(0) nanoparticles were obtained in high densities from a novel type reverse micelle (or W/O microemulsion) of bis(N-octylethylenediamine)palladium(II) chloride (=[Pd(octen)(2)]Cl-2)/water/chloroform (= CHCl3). The shape of the nanoparticles was largely affected by the phase of the additive, sodium boron hydride (= NaBH4) reductant, and by the replacement of a small amount of water with methanol., CHEMICAL SOC JAPAN, Oct. 2002, CHEMISTRY LETTERS, 10 (10), 1050 - 1051, web_of_science

    Scientific journal

  • A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan

    Niichiro Abe; Mizuho Nagoshi; Kazutoshi Takami; Yoshinori Sawano; Hisao Yoshikawa

    Elsevier BV, Jun. 2002, Veterinary Parasitology, 106 (3), 203 - 212, doi;web_of_science

    Scientific journal

  • Phylogenetic Position of Blastocystis hominis and of Stramenopiles Inferred from Multiple Molecular Sequence Data

    NOBUKO ARISUE; TETSUO HASHIMOTO; HISAO YOSHIKAWA; YOSHIfflRO NAKAMURA; GEN NAKAMURA; FUMINORI NAKAMURA; TAKA-AKI YANO; MASAMI HASEGAWA

    Wiley, Jan. 2002, The Journal of Eukaryotic Microbiology, 49 (1), 42 - 53, doi;web_of_science

    Scientific journal

  • Genomic Analysis of Blastocystis hominisStrains Isolated from Two Long-Term Health Care Facilities

    Hisao Yoshikawa; Niichiro Abe; Mizue Iwasawa; Syoko Kitano; Isao Nagano; Zhiliang Wu; Yuzo Takahashi

    The genotype Blastocystis hominis is highly polymorphic. Therefore, a genetic marker would be a powerful tool for the identification or classification of B. hominis subtypes and could be used as a means to resolve the transmission route or origin of the parasite. To this end, 32 B. hominis isolates were collected from patients and/or staff members of two long-term health care facilities (facilities A and B), and these organisms were subjected to genotype analysis based on diagnostic PCR primers and restriction fragment length polymorphism (RFLP) of small subunit rRNA gene (rDNA). Based on PCR amplification using diagnostic primers which were developed from randomly amplified polymorphic DNA analysis of known strains of B. hominis, the 32 isolates of B. hominis were classified into three different subtypes. Thirty isolates, including twenty-four that were isolated from patients and a staff member, from facility A and all isolates isolated from six patients from facility B showed the same genotype. Two of six patients of facility B had been transferred from facility A, and these two patients also had the same-genotype B. hominis that corresponded to 24 isolates from facility A. This genotype strain may have been transmitted by these two patients from facility A to facility B, suggesting human-to-human transmission. In contrast, 2 of 26 isolates from facility A showed distinct genotypes, suggesting that the colonization by these two isolates is attributable to another infectious route. These different subtypes were subjected to RFLP analysis, and the RFLP profiles were correlated with the results obtained by diagnostic PCR primers. This study presents the first molecular evidence of possible human-to-human B. hominisinfection between and/or among two small communities., American Society for Microbiology, 2000, Journal of Clinical Microbiology, 38 (4), 1324 - 1330, doi;web_of_science

    Scientific journal

  • Genomic polymorphism amongBlastocystis hominisstrains and development of subtype-specific diagnostic primers

    Hisao Yoshikawa; Isao Nagano; Zhiliang Wu; Eu Hian Yap; Mulkit Singh; Yuzo Takahashi

    Elsevier BV, Jun. 1998, Molecular and Cellular Probes, 12 (3), 153 - 159, doi;web_of_science

    Scientific journal

  • Freeze-fracture studies of Cryptosporidium muris.

    YOSHIKAWA Hisao; H. Yoshikawa; M. Iseki

    1998, Journal of Protozoology, 38 (6), 171S-172S

  • Isolation and characterization of Blastocystis spp. isolated from anuran amphibians

    H Yoshikawa; K Morimoto; M Nagashima

    Blastocystis spp. were isolated from three species of anuran amphibians in Japan. Eleven strains of Blastocystis sp were isolated from 13 frogs of Rana catesbeiana. Four strains of Blastocystis sp. were isolated from 21 frogs of Rana nigromaculata. All of 5 frogs of Bufo japonicus japonicus were positive of Blastocystis sp. Culturing of the organisms at various temperatures and temperature tolerance assays indicated that two strains isolated from a R. nigromaculata and a B. japonicus japonicus were different from Blastocystis hominis and Blastocystis lapemi isolated from a reptile. Based on the difference of temperature tolerance, two strains isolated from frogs might be new species of Blastocystis., MEDIMOND S R L, 1998, ICOPA IX - 9TH INTERNATIONAL CONGRESS OF PARASITOLOGY, 1075 - 1079, web_of_science

    International conference proceedings

  • Cyst form of Blastocystis pythoni in vitro culture

    H Yoshikawa; M Nagashima; K Morimoto

    IOP PUBLISHING LTD, 1998, ELECTRON MICROSCOPY 1998, VOL 4, 403 - 404, web_of_science

    International conference proceedings

  • Heterogeneous Distribution of Membrane Cholesterol at the Attachment Site of Cryptosporidium muris to Host Cells

    HISAO YOSHIKAWA; ISAO KIMATA; MOTOHIRO ISEKI

    Wiley, Sep. 1997, The Journal of Eukaryotic Microbiology, 44 (5), 454 - 456, doi;web_of_science

    Scientific journal

  • Ultrastructural localization of basic proteins of Blastocystis hominis

    H Yoshikawa; K Oishi

    Basic proteins of Blastocystis hominis were detected by the ammoniacal silver and ethanolic phosphotungstic acid techniques using electron microscopy. The central vacuole showed many silver grains when treated with ammoniacal silver and an increased electron density when treated with phosphotungstic acid. The intensity of positive reactions correlated with the electron density of the central vacuole, because cells having an electron-lucent central vacuole showed no silver grain deposits. Since it is known that the concentration of electron-dense materials in the central vacuole increases during log phase of growth, and then decreases in stationary phase, this organelle must accumulate basic proteins during cell growth., SPRINGER WIEN, 1997, PROTOPLASMA, 200 (1-2), 31 - 34, web_of_science

    Scientific journal

  • Freeze-fracture Cytochemistry of Membrane Cholesterol in Blastocystis hominis

    H. Yoshikawa; A. Hayakawa

    Elsevier BV, Oct. 1996, International Journal for Parasitology, 26 (10), 1111 - 1114, doi;web_of_science

    Scientific journal

  • Blastocystis hominis infection

    RE Boreham; S Benson; DJ Stenzel; PFL Boreham

    LANCET LTD, Jul. 1996, LANCET, 348 (9022), 272 - 273, web_of_science

  • Phylogenetic position of Blastocystis hominis that contains cytochrome-free mitochondria, inferred from the protein phylogeny of elongation factor 1α

    Yoshihiro Nakamura; Tetsuo Hashimoto; Hisao Yoshikawa; Takashi Kamaishi; Fuminori Nakamura; Ken-ichi Okamoto; Masami Hasegawa

    Elsevier BV, May 1996, Molecular and Biochemical Parasitology, 77 (2), 241 - 245, doi;web_of_science

    Scientific journal

  • DNA Polymorphism Revealed by Arbitrary Primers Polymerase Chain Reaction AmongBlastocystisStrains Isolated from Humans, a Chicken, and a Reptile

    HISAO YOSHIKAWA; ISAO NAGONO; EU HIAN YAP; MULKIT SINGH; YUZO TAKAHASHI

    Wiley, Mar. 1996, Journal of Eukaryotic Microbiology, 43 (2), 127 - 130, doi;web_of_science

    Scientific journal

  • Morphological changes in the central vacuole of Blastocystis hominis during in vitro culture

    H Yoshikawa; A Hayakawa

    Morphological changes in the central vacuole during the growth in in vitro culture of Blastocystis hominis were investigated by light and electron microscopy. Most cells in log phase and an early stationary phase showed a positive staining reaction in the central vacuole with PAS or Sudan black B stain, whereas cells in late stationary phase showed few positive reactions. Electron microscopic observations revealed that 95% of B. hominis cells in log phase and 50% of cells in early stationary phase, had a substantial accumulation of electron-dense material in the central vacuole. In contrast, only 25% of the organisms in late stationary phase had an electron-dense central vacuole, while more than 50% of cells had an electron-lucent central vacuole. These results indicate that B. hominis accumulated carbohydrates and lipids in the central vacuole during cell growth and that the organism probably consumed these metabolic substances during stationary growth. Therefore, it is strongly suggested that the central vacuole is an important organelle for storage of metabolic substances, such as carbohydrates and lipids, required for cell growth., SPRINGER WIEN, 1996, PROTOPLASMA, 194 (1-2), 63 - 68, web_of_science

    Scientific journal

  • Antigenic differences between Blastocystis hominis and Blastocystis sp revealed by polyclonal and monoclonal

    H Yoshikawa; Katagiri, I; XH Li; M BeckerHapak; DC Graves

    The antigenic differences between Blastocystis hominis and Blastocystis sp. (isolated from a human and a chicken sources, respectively) were investigated by using polyclonal and monoclonal antibodies, Polyclonal antibodies did not reveal distinct antigenic differences between the strains by Western blot analysis, whereas monoclonal antibodies (MAbs) produced against B. hominis did detect antigenic polymorphism between B. hominis and Blastocystis sp, Six out of 8 MAbs were specific to B. hominis antigens and 2 MAbs reacted with both B. hominis and Blastocystis sp, antigens, Immunoelectron microscopy using these MAbs, revealed labeling on the filamentous layer or the central vacuole, Interestingly, the 6 MAbs reacted only with B. hominis antigens on the filamentous layer, whereas the other 2 MAbs labeled on the central vacuole, Therefore, the antigenic components of the filamentous layer may be strain or species specific. In contrast, the antigenic components of the central vacuole are shared by both B. hominis and Blastocystis sp, suggesting the metabolic substances in the central vacuole are antigenically common., NATL RESEARCH CENTER PROTOZOAN DISEASES, Jul. 1995, JOURNAL OF PROTOZOOLOGY RESEARCH, 5 (3), 118 - 128, web_of_science

    Scientific journal

  • LIGHT AND ELECTRON-MICROSCOPIC LOCALIZATION OF LIPIDS IN BLASTOCYSTIS-HOMINIS

    H YOSHIKAWA; J SATOH; Y ENOSE

    Localization of the lipid of Blastocystis hominis was histochemically demonstrated, B. hominis was fixed with glutaraldehyde, treated with detergents, and stained with Sudan black B or Nile blue. The central vacuole of B. hominis stained with Sudan black B showed many black droplets with great variation in staining intensity. In Nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. These results indicated the presence of neutral lipids and/or acidic lipids in the central vacuole, At the ultrastructural level, the central vacuole and some cytoplasmic vesicles showed enhanced electron density after post-fixation with imidazole-buffered osmium tetroxide solution, Great variation in the density and distribution of the electron-dense materials was observed in the central vacuole. Since the electron-dense vesicles were frequently observed in the cytoplasm, B. hominis may accumulate the lipid in the central vacuole, the function of which is not well known., JAPANESE SOC ELECTRON MICRO BUS CENTER ACAD SOC JAPAN, Apr. 1995, JOURNAL OF ELECTRON MICROSCOPY, 44 (2), 100 - 103, web_of_science

    Scientific journal

  • Histochemical Detection of Carbohydrates of Blastocystis hominis

    HISAO YOSHIKAWA; NOBUKO KUWAYAMA; YOSHIMI ENOSE

    Wiley, Jan. 1995, The Journal of Eukaryotic Microbiology, 42 (1), 70 - 74, doi;web_of_science

    Scientific journal

  • DISTRIBUTION OF FILIPIN-STEROL COMPLEXES IN THE BLOOD-STREAM FORM OF TRYPANOSOMA-BRUCEI-GAMBIENSE

    H YOSHIKAWA; J FURUKI; Y TAKAHASHI; H MORIOKA; Y YOSHIDA

    The polyene antibiotic, filipin, was used as a probe for demonstrating sterols in the plasma membrane of Trypanosoma brucei gambiense. Three different regions of the continuous plasma membrane, over the cell body proper, flagellar pocket, and flagellum, were compared as to density and distribution of the filipin-sterol complexes by freeze-fracture method. The density of the complexes was highest in the cell body membrane and lowest over the flagellar pocket. The filipin-sterol complexes in the cell body membrane were distributed homogeneously on both the protoplasmic and exoplasmic faces except in the zone of flagellar attachment. This junctional zone showed a linear, complex-poor region. In the flagellar membranes, a line of complex-poor regions was observed along the juncture of the flagellum to the cell body. At the neck of the flagellar pocket, the membranes of the flagellar pocket and flagellum were closely opposed, with few filipin-sterol complexes on either membrane. At the base of the flagellar shaft, the complexes were completely lacking on both faces. Based on these observations, the zones of juncture observed in T. b. gambiense seems to be similar in ultrastructure to mammalian cell junctions. such as tight, gap, septate junctions and desmosomes, which show a nearly complete absence of the filipin-sterol complexes., OXFORD UNIV PRESS UNITED KINGDOM, Oct. 1992, JOURNAL OF ELECTRON MICROSCOPY, 41 (5), 364 - 368, web_of_science

    Scientific journal

  • Freeze-Fracture Study of the Site of Attachment ofCryptosporidium murisin Gastric Glands

    HISAO YOSHIKAWA; MOTOHIRO ISEKI

    Wiley, Sep. 1992, The Journal of Protozoology, 39 (5), 539 - 544, doi;web_of_science

    Scientific journal

  • FREEZE-FRACTURE STUDIES OF CRYPTOSPORIDIUM-MURIS

    H YOSHIKAWA; M ISEKI

    The attachment site of Cryptosporidium muris to host cells was investigated using the freeze-fracture method. Cryptosporidium muris was enveloped by a double membrane of host plasma membrane origin, which formed the parasitophorous vacuole. The outer membrane of the double membrane was continuous with the host plasma membrane at the dense band, while the inner membrane was connected with the anterior part of the parasite plasma membrane at the annular ring. The density of intramembranous particles (IMP) was dramatically altered at the above two junctures. The outer parasitophorous membrane showed low IMP-density as compared to the host plasma membrane, although both membranes were continuous. The inner parasitophorous membrane had few IMP, whereas the parasite plasma membrane showed numerous IMP. When the attachment sites of parasites and host cells were fractured, circular-shaped fractured faces were observed on both sites of the parasite and host cell. These exposed faces corresponded to the dense bands and were very similar in size in each parasite., SOC PROTOZOOLOGISTS, Nov. 1991, JOURNAL OF PROTOZOOLOGY, 38 (6), S171 - S172, web_of_science

  • ULTRASTRUCTURAL-LOCALIZATION OF EPITOPES RECOGNIZED BY MONOCLONAL-ANTIBODIES PRODUCED TO RAT PNEUMOCYSTIS-CARINII

    H YOSHIKAWA; LE DEBAULT; DC GRAVES

    The binding sites of five monoclonal antibodies (MAb) developed against rat Pneumocystis carinii were examined at the ultrastructural level by using a post-embedding labeling method. Although all five MAb reacted with the pellicle of P. carinii, they were divided into two groups by localization of binding sites. The MAb 168.2.1, 174.2.1, and 215.2.1 reacted mainly with the electron-dense outer layer, whereas MAb 227.1.1 and 228.1.1 labeled both the outer dense layer and the middle lucent layer. With in the first group of MAb, no significant differences were observed in the reactivity patterns seen with the different stages of P. carinii. In the second group, however, the intensity of labeling of the electron-dense layer was higher in the precyst, cyst, and ruptured cyst stages than in the trophozoite stage. These latter results indicate that there may be an increase in antigen accumulation during development from the trophozoite to the cyst stages, or that antigens may be modified the development., SOC PROTOZOOLOGISTS, Nov. 1991, JOURNAL OF PROTOZOOLOGY, 38 (6), S18 - S20, web_of_science

  • Freeze-Fracture Study of the Bloodstream Form of Trypanosoma brucei gambiense

    HISAO YOSHIKAWA; JUNKO FURUKI; YUZO TAKAHASHI; HIROYUKI MORIOKA; YUKIO YOSHIDA

    Wiley, Jan. 1990, The Journal of Protozoology, 37 (1), 27 - 32, doi;web_of_science

    Scientific journal

  • Bromodeoxyuridine labeling studies on the proliferation of intestinal mucosal mast cells in normal and athymic rats

    NAOKI Arizono; TSUNEZO Shiota; MINORU Yamada; YOSHITSUGU Matsumoto; HISAO Yoshikawa; SHINJI Matsuda; TATSUYA Tegoshi

    Wiley, Jan. 1990, APMIS, 98 (1-6), 369 - 376, doi;web_of_science

    Scientific journal

  • Disseminated infection ofPneumocystis carinii in a patient with the acquired immunodeficiency syndrome

    Shinji Matsuda; Yohji Urata; Tsunezo Shiota; Minoru Yamada; Hisao Yoshikawa; Tatsuya Tegoshi; Mitsunori Okada; Hayato Nakamura; Toshio Kitaoka; Tsukasa Ashihara; Yukio Yoshida

    Springer Science and Business Media LLC, 1989, Virchows Archiv A Pathological Anatomy and Histopathology, 414 (6), 523 - 527, doi;web_of_science;url;url;url

    Scientific journal

  • Variations in egg size ofTrichuris trichiura

    H. Yoshikawa; M. Yamada; Y. Matsumoto; Y. Yoshida

    Springer Science and Business Media LLC, 1989, Parasitology Research, 75 (8), 649 - 654, doi;web_of_science;url;url;url

    Scientific journal

  • The Present Status ofPneumocystis cariniiPneumonia in Japan with Special Reference to AIDS

    YUKIO YOSHIDA; TSUXEZO SHIOTA; MINORU YAMADA; HISAO YOSHIKAWA; TATSUYA TEGOSHI; TSUYOSHI IKAI; KENJI OGINO

    Wiley, Jan. 1989, The Journal of Protozoology, 36 (1), 78S - 80S, doi;web_of_science

    Scientific journal

  • Detection of Surface Carbohydratesof Pneumocystis carinii

    HISAO YOSHIKAWA; TATSUYA TEGOSHI; YUKIO YOSHIDA

    Wiley, Jan. 1989, The Journal of Protozoology, 36 (1), 63S - 64S, doi;web_of_science

    Scientific journal

  • Ultrastructure of Pneumocystis carinii: A Freeze-Fracture Study

    HISAO VOSHIKAWA; YUKIO YOSHIDA

    Wiley, Jan. 1989, The Journal of Protozoology, 36 (1), 51S - 52S, doi;web_of_science

    Scientific journal

  • Freeze-Fracture Study of Blastocystis hominis

    HISAO YOSHIKAWA; MINORU YAMADA; YUKIO YOSHIDA

    Wiley, Nov. 1988, The Journal of Protozoology, 35 (4), 522 - 528, doi;web_of_science

    Scientific journal

  • Freeze-Fracture Study of the Trophozoite and the Cyst ofEntamoeba histolytica

    HISAO YOSHIKAWA; MINORU YAMADA; YUKIO YOSHIDA

    Wiley, May 1988, The Journal of Protozoology, 35 (2), 268 - 273, doi;web_of_science

    Scientific journal

  • Distribution of digitonin-sterol complexes in Pneumocystis carinii revealed by freeze-fracture method

    Hisao Yoshikawa; Yukio Yoshida

    Elsevier BV, Feb. 1988, International Journal for Parasitology, 18 (1), 39 - 45, doi;web_of_science

    Scientific journal

  • Ultrastructural detection of carbohydrates in the pellicle of Pneumocystis carinii

    H. Yoshikawa; H. Morioka; Y. Yoshida

    Springer Science and Business Media LLC, 1988, Parasitology Research, 74 (6), 537 - 543, doi;web_of_science;url;url;url

    Scientific journal

  • FREEZE-FRACTURE LOCALIZATION OF FILIPIN-STEROL COMPLEXES IN PLASMA-MEMBRANES AND CYTO-MEMBRANES OF PNEUMOCYSTIS-CARINII

    H YOSHIKAWA; H MORIOKA; Y YOSHIDA

    SOC PROTOZOOLOGISTS, May 1987, JOURNAL OF PROTOZOOLOGY, 34 (2), 131 - 137, web_of_science

    Scientific journal

  • LOCALIZATION OF SILVER DEPOSITS ON PNEUMOCYSTIS-CARINII TREATED WITH GOMORI METHENAMINE SILVER-NITRATE STAIN

    H YOSHIKAWA; Y YOSHIDA

    GUSTAV FISCHER VERLAG, May 1987, ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, 264 (3-4), 363 - 372, web_of_science

    Scientific journal

  • LIGHT MICROSCOPIC STUDY OF BLASTOCYSTIS SPP IN MONKEYS AND FOWLS

    M YAMADA; H YOSHIKAWA; T TEGOSHI; Y MATSUMOTO; T YOSHIKAWA; T SHIOTA; Y YOSHIDA

    SPRINGER VERLAG, 1987, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 73 (6), 527 - 531, web_of_science

    Scientific journal

  • Effect of working time and temperature of sulfuric ether in the Toluidine Blue O stain for Pneumocystis carinii. (共著)

    YOSHIKAWA Hisao; M. Yamada

    1987, The Japanese Journal of Parasitology, 36 (6), 371-374

  • DETECTION OF SURFACE CARBOHYDRATES ON PNEUMOCYSTIS-CARINII BY FLUORESCEIN-CONJUGATED LECTINS

    H YOSHIKAWA; T TEGOSHI; Y YOSHIDA

    SPRINGER VERLAG, 1987, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 74 (1), 43 - 49, web_of_science

    Scientific journal

  • FREEZE-FRACTURE STUDIES ON PNEUMOCYSTIS-CARINII .2. FINE-STRUCTURE OF THE TROPHOZOITE

    H YOSHIKAWA; H MORIOKA; Y YOSHIDA

    SPRINGER VERLAG, 1987, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 73 (2), 132 - 139, web_of_science

    Scientific journal

  • Clinical trial of trimethoprim-sulfamethoxazole in the treatment of vivax, ovale and falciparum malaria. (共著)

    YOSHIKAWA Hisao; Y. Yoshida

    1987, Japan. J. Trop. Med. Hyg., 15 (2), 105-116

  • FREEZE-FRACTURE STUDIES ON PNEUMOCYSTIS-CARINII .3. FINE-STRUCTURE OF THE PRECYST AND THE CYST

    H YOSHIKAWA; Y YOSHIDA

    SPRINGER VERLAG, 1987, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 74 (1), 36 - 42, web_of_science

    Scientific journal

  • FREEZE-FRACTURE STUDIES ON PNEUMOCYSTIS-CARINII .1. STRUCTURAL ALTERATION OF THE PELLICLE DURING THE DEVELOPMENT FROM TROPHOZOITE TO CYST

    H YOSHIKAWA; Y YOSHIDA

    SPRINGER VERLAG, 1986, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, 72 (4), 463 - 477, web_of_science

    Scientific journal

  • Trimethoprim-SulfamethoxazoleおよびPyrimethamine-SulfamonomethoxineによるPneumocystis carinii肺炎の発症予防に関する実験的研究 (共著)

    YOSHIKAWA Hisao

    1985, 日本熱帯医学会雑誌, 13 (4), 287-294

  • PNEUMOCYSTIS-CARINII - ELECTRON-MICROSCOPIC INVESTIGATION ON THE INTERACTION OF TROPHOZOITE AND ALVEOLAR LINING CELL

    Y YOSHIDA; Y MATSUMOTO; M YAMADA; K OKABAYASHI; H YOSHIKAWA; M NAKAZAWA

    GUSTAV FISCHER VERLAG, 1984, ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, 256 (3), 390 - 399, web_of_science

    Scientific journal

  • Report of an imported case of Dipetalonema perstans in Japan. (共著)

    YOSHIKAWA Hisao; Y. Yoshida

    1983, Southeast Asian Journal Trop. Med. Pub. Hlth., 14 (3), 341-344

MISC

  • 腸管寄生原虫類の遺伝子検査法

    YOSHIKAWA Hisao

    Jun. 2012, 検査と技術, 40 (6), 478-486

  • 腸管寄生原虫ブラストシスチスとその検査法

    YOSHIKAWA Hisao

    Jan. 2011, 検査と技術, 39 (1), 11-17

  • 最近注目され始めた腸管寄生原虫Blastocystis hominisの検査法と鑑別診断法

    YOSHIKAWA Hisao

    Aug. 1997, 臨床検査, 41 (8), 938-943

  • フリーズレプリカ装置FR-7000を用いた応用例

    YOSHIKAWA Hisao

    Mar. 1997, Hitachi Scienetific Instrument News, 39 (2), 14-17

  • プラジカンテルによる肝吸虫症の治療 (共著)

    YOSHIKAWA Hisao

    1991, 診断と治療, 79 (1), 155-158

  • フリーズフラクチャー法による寄生原虫の研究

    YOSHIKAWA Hisao

    1989, 医学のあゆみ, 148 (2), 84

  • キロショウジョウバエ温度感受性突然変異Shibirets1の翅原基の腫瘍化 (共著)

    YOSHIKAWA Hisao

    1988, Memoris of the Konan University Science Series, 35 (1), 31-38

Books etc

  • Materials and Methods in Parasitology

    YOSHIKAWA Hisao (, Range: 分担執筆)

    三恵社, 2013, 1-4; 21-24

  • Parasitology Research Monographs 4: Blastocystis:Pathogen or Passenger? An evaluation of 101 years of research

    Heinz Mehlhorn; Kevin S.W. Tan; Hisao Yoshikawa (, Range: Editor & Author)

    Springer, Dec. 2012, 編集者&執筆者

  • Molecular Detection of Human Parasitic Pathogens. Blastocystis

    YOSHIKAWA Hisao (, Range: Author)

    CRC Press, Jul. 2012, 39-51

  • スタンダード微生物学(第2版)

    YOSHIKAWA Hisao (, Range: 分担執筆)

    文光堂, Feb. 2008, 350-362

  • Parasitic Zoonosis in Asian-Pacific Regions 2006

    YOSHIKAWA Hisao (, Range: 分担執筆)

    Sankei-sha Co. Ltd., Dec. 2006, 16, 20-31

  • スタンダード微生物学

    YOSHIKAWA Hisao (, Range: 分担執筆)

    文光堂, Mar. 2005, 351-362

  • 虫の知らせ

    YOSHIKAWA Hisao (, Range: 分担執筆)

    三恵社, Jul. 2002

  • Isolation and characterization of Blastocystis spp. isolated from anuran amphibians(共著)

    H. Yoshikawa; K. Morimoto; M. Nagashima (, Range: pp. 1075-1079.)

    9th International Corgress of Parasitology. International Proceedings Division., 1998, 1075-1079頁

  • Cyst form of Blastocystis pythoni in vitro culture. (共著)

    H. Yoshikawa; M. Nagashima; K. Morimoto (, Range: pp. 403-404)

    Electron Microscopy Vol. IV Biological Sciences, 1998, 4巻403-404頁

  • 図説細胞生物学

    YOSHIKAWA Hisao (, Range: 分担執筆)

    丸善, 1998, 150-159

Presentations

  • Blastocystisのゲノム上のSSU rRNA遺伝子コピー間の 塩基配列の違いについて: ST6とST7に分類されるヒト由来株と鳥類由来株間の比較

    横江彩子、吉川尚男

    第75回日本寄生虫学会西日本支部大会, 21 Sep. 2019, 21 Sep. 2019, 22 Sep. 2019

  • 複数のサブタイプを混合培養した際のBlastocystisの増殖について

    辻本あかり、吉川尚男

    第75回日本寄生虫学会西日本支部大会, 21 Sep. 2019, 21 Sep. 2019, 22 Sep. 2019

  • ブラストシスチス属の人獣共通感染性、インドネシア・スンバ島の10家族の住民とその家畜動物から分離された株について

    YOSHIKAWA Hisao

    第88回日本寄生虫学会大会, Mar. 2019, 長崎大学

  • Blastocystis and zoonotic potential: Molecular epidemiological approach to residents and their domestic animals among 10 families in a small Indonesian community with poor hygiene.

    Hisao Yoshikawa

    The 2nd International Blastocystis Conference, 12 Sep. 2018, 11 Sep. 2018, 12 Sep. 2018

  • Molecular study of Blastocystis isolates from residents and their domestic animals among 10 families in a small Indonesian community with poor hygiene.

    H. Yoshikawa; A. Iwamasa; M. Tokoro; T. Nagamoto; D. Syafruddin

    15th International Congress of Protistology, 01 Aug. 2017, rm:research_project_id

  • 奈良市内の環境水からのアカントアメーバの分離と遺伝子型解析

    YOSHIKAWA Hisao

    第86回日本寄生虫学会大会, May 2017, 北海道大学・学術交流会館

  • インドネシア・スンバ島の住民と家畜動物間のブラストシスチス感染伝播について(2)

    YOSHIKAWA Hisao

    第72回日本寄生虫学会西日本支部大会, Oct. 2016, 岐阜市

  • インドネシア・スンバ島で捕獲したゴキブリから分離されたブラストシスチス株について

    YOSHIKAWA Hisao

    第72回日本寄生虫学会西日本支部大会, Oct. 2016

  • インドネシアで分離されたヒト・ブラストシスチス分離株のゲノム多型について

    YOSHIKAWA Hisao

    第72回日本寄生虫学会西日本支部大会, Oct. 2016, 岐阜市

  • カエルから分離されたブラストシスチスの分子系統解析

    YOSHIKAWA Hisao

    第72回日本寄生虫学会西日本支部大会, Oct. 2016, 岐阜市

  • 環境水からのアカントアメーバの分離の試み

    YOSHIKAWA Hisao

    第72回日本寄生虫学会西日本支部大会, Oct. 2016, 岐阜市

  • ブラストシスチス属の人獣共通感染性、インドネシア・スンバ島住民とその家畜から分離された株について

    YOSHIKAWA Hisao

    第85回日本寄生虫学会大会, Mar. 2016, 宮崎

  • 爬虫類から分離したブラストシスチス株の分子系統解析

    YOSHIKAWA Hisao

    第71回日本寄生虫学会西日本支部大会, Oct. 2015, 奈良

  • インドネシア・スンバ島の住民と家畜動物間のブラストシスチス感染伝播について

    YOSHIKAWA Hisao

    第71回日本寄生虫学会西日本支部大会, Oct. 2015, 奈良

  • Human Blastocystis Subtyping by a Combination of RFLP Analysis and Subtype-Specific Primers

    Hisao Yoshikawa, Ayana Iwamasa

    The 1 st International Blastocystis Symposium, 29 May 2015, 28 May 2015, 29 May 2015

  • Host Specificity of Blastocystis Subtypes: Comparison of Humans with Domestic Animals in a Small Community in Sumba Island, Indonesia

    Hisao Yoshikawa, Masaharu Tokoro, Din Syafulddin

    The 1 st International Blastocystis Symposium, 29 May 2015, 28 May 2015, 29 May 2015

  • Blastocystis phylogeny among a variety isolates from humans to insects.

    Hisao Yoshikawa

    The 1st International Blastocystis Symposium., 28 May 2015, 28 May 2015, 29 May 2015

  • 遺伝的多型のヒトブラストシスチス株を簡便にサブタイプ分類するためのプライマー構築

    YOSHIKAWA Hisao

    第84回日本寄生虫学会大会, Mar. 2015, 杏林大学医学部

  • 爬虫類由来株のブラストシスチスの遺伝的多様性について

    YOSHIKAWA Hisao

    第70回日本寄生虫学会西日本支部大会, Oct. 2014, 兵庫医療大学薬学部微生物学分野, 神戸市・兵庫医療大学

  • ブラストシスチス株の簡便なサブタイプ分類と系統解析のためのプライマー設計

    YOSHIKAWA Hisao

    第70回日本寄生虫学会西日本支部大会, Oct. 2014, 兵庫医療大学薬学部微生物分野, 神戸市・兵庫医療大学

  • Host-specificity of Blastocystis subtypes: Comparison of humans with domestic pigs and wild rodents in a small community in Sumba Island, Indonesia

    Hisao Yoshikawa; Masaharu Tokoro; Din Syafruddin

    13th International Congress of Parasitology, 12 Aug. 2014

  • Genetic diversity of Giardia intestinalis detected from humans: Is there any geographical distribution pattern?

    Masaharu Tokoro; Hisao Yoshikawa; Din Syafruddin

    13th International Congress of Parasitology, 11 Aug. 2014

  • ブラストシスチス属の遺伝的多様性と宿主特異性:特に日本とインドネシアのげっ歯類から分離された株について

    YOSHIKAWA Hisao

    第83回日本寄生虫学会大会, Mar. 2014, 愛媛大学

  • 爬虫類由来ブラストシスチス株の性状と遺伝的多様性

    YOSHIKAWA Hisao

    第69回日本寄生虫学会西日本支部大会, Oct. 2013, 高松市、香川

  • 日本とインドネシアのげっ歯類由来のブラストシスチス株の遺伝的多様性

    YOSHIKAWA Hisao

    第69回日本寄生虫学会西日本支部大会, Oct. 2013, 高松市、香川

  • 糞便検体からのDNA精製効率改善を目指した試み

    YOSHIKAWA Hisao

    第69回日本寄生虫学会西日本支部大会, Oct. 2013, 高松市、香川

  • Spcificity of PCRprimers developed for Blastocystis subtype identification

    YOSHIKAWA Hisao; Hisao Yoshikawa; Masaharu Tokoro; Din Syafruddin

    24th Molecular Parasitology Meeting, Sep. 2013

  • A comprehensive molecular analysis for various intestinal protozoan parasites.

    YOSHIKAWA Hisao; Masaharu Tokoro; Hisao Yoshikawa; Din Syafruddin

    24th Molecular Parasitology Meeting, Sep. 2013

  • A Unique Intestinal Protozoan Parasite, Blastocystis spp. --Epidemiology and Zoonotic Potential--

    YOSHIKAWA Hisao; Hisao Yoshikawa

    Internation Workshop on Enteric Protozoan Infections, Feb. 2013, National Institute of Infectious Diseases, 東京

  • A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia.

    YOSHIKAWA Hisao; M. Tokoro; H. Yoshikawa; D. Syafruddin

    The 12th Asia-Pacific Congree for Parasitic Zoonoses, Oct. 2012, Kobe, Japan

  • Molecular phylogeny of Blastocystis isolates from rodents in the Sumba Island, Indonesia

    YOSHIKAWA Hisao; M. Katsumata; H. Yoshikawa; M. Tokoro; D. Syafruddin

    The 12th Asia-Pacific Congree for Parasitic Zoonoses, Oct. 2012, Kobe, Japan

  • Improvement of molecular detection methods for intestinal protozoan parasites.

    YOSHIKAWA Hisao; S. Arayama; S. Nakazawa; H. Yoshikawa; M. Tokoro

    The 12th Asia-Pacific Congree for Parasitic Zoonoses, Oct. 2012, Kobe, Japan

  • ブラストシスチスのサブタイプ同定に用いられているPCRプライマーの特異性について

    YOSHIKAWA Hisao

    第68回日本寄生虫学会西日本支部大会, Oct. 2012, 奈良

  • 動物園で飼育されている爬虫類からのブラストシスチス株分離の試み

    YOSHIKAWA Hisao

    第68回日本寄生虫学会西日本支部大会, Oct. 2012, 奈良

  • 爬虫類由来ブラストシスチス株の性状と遺伝的多様性について

    YOSHIKAWA Hisao

    第68回日本寄生虫学会西日本支部大会, Oct. 2012, 奈良

  • A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia.

    YOSHIKAWA Hisao; M. Tokoro; H. Yoshikawa; D. Syafruddin

    15th International Congress of Infectious Diseases, Jun. 2012, Bangkok, Thailand

  • Entamoeba dispar感染にヒトブラストシスチス及び小形アメーバが検出されたブラジル人症例と回盲部に潰瘍を認めた無症候性E. histolytica感染の日本人症例

    YOSHIKAWA Hisao

    第23回日本臨床寄生虫学会, Jun. 2012, 東京

  • Comprehensive molecular detection methods revealed a true image of protozoan endemicity in Southwest Sumba district, Indonesia

    YOSHIKAWA Hisao

    第81回日本寄生虫学会大会, Mar. 2012, 日本寄生虫学会, 兵庫医科大学、西宮

  • ブラストシスチスの感染伝播に関する研究:2009年に実施したインドネシア・スンバ島での調査

    YOSHIKAWA Hisao

    第81回日本寄生虫学会大会, Mar. 2012, 日本寄生虫学会, 兵庫医科大学、西宮

  • Diversity of the genus Blastocystis

    YOSHIKAWA Hisao; Hisao Yoshikawa

    University of Leicester, College of Medicine, Biological Science & Psychology, Nov. 2011, Leicester, England

  • 蛍光抗体試薬のブラストシスチス検出感度について

    YOSHIKAWA Hisao

    第67回日本寄生虫学会西日本支部大会, Oct. 2011, 日本寄生虫学会, 金沢大学

  • サブタイプ1から9までのブラストシスチス株のミトコンドリア遺伝子ND3による系統解析

    YOSHIKAWA Hisao

    第67回日本寄生虫学会西日本支部大会, Oct. 2011, 日本寄生虫学会, 金沢大学

  • ブラストシスチス属の分子分類の現状と問題点

    YOSHIKAWA Hisao

    第67回日本寄生虫学会西日本支部大会, Oct. 2011, 所 正治, 金沢大学

  • Molecular phylogenetic studies of Blastocystis isolates from manrsupial mammals.

    YOSHIKAWA Hisao; Hisao Yoshikawa; Mayumi Murakami; Zhiliang Wu; Tetsuo Yanagi

    17th Japanese-German Cooperative Symposium on Protozoan Diseases and Workshop of Gastrointestinal Protozoan Diseases, Sep. 2011, The Japanese-German Association on Protozoan Diseases, Nara Women's University, Nara

  • Massive waterborne outbreaks of cryptosporidiosis in Japan

    YOSHIKAWA Hisao; Norishige Yamamoto; Marina Kon; Mahaharu Tokoro; Motohiro Iseki; Isao Kimata; Hisao Yoshikawa

    17th Japanese-German Cooperative Symposium on Protozoan Diseases and Workshop of Gastrointestinal Protozoan Diseases, Sep. 2011, The Japanese-German Association on Protozoan Diseases, Nara Women's University, Nara

  • ミトコンドリアNADHデヒドロゲナーゼND3遺伝子によるブラストシスチス株の分子系統解析

    YOSHIKAWA Hisao

    第80回日本寄生虫学会大会, Jul. 2011, 日本寄生虫学会, 東京慈恵会医科大学、東京

  • Comparison of commercial DNA isolation kits for molecular diagosis of Blastocystis protozoon with culture method.

    YOSHIKAWA Hisao; H. Yoshikawa; F Dogruman-AI; S. Turk; S. Kustimur; N. Balaban; N. Sultan

    XXXIV Turkish Microbiology Congress, Sep. 2010

  • ブラストシスチスの診断・検出法の評価:市販キットによる糞便からの直接DNA抽出-PCR法と培養法の比較

    YOSHIKAWA Hisao

    第79回日本寄生虫学会大会, May 2010, 日本寄生虫学会, 北海道、旭川市

  • Diversity and phylogeny in the genus Blastocystis

    YOSHIKAWA Hisao; Hisao Yoshikawa

    Gazi University in Turkey, Sep. 2009, Department of Medical Microbiology, Gazi University in Turkey, Ankara

  • トルコの3病院下痢症患者と無症状患者におけるブラストシスチス感染状況と分離株のサブタイプ分類

    YOSHIKAWA Hisao

    第78回日本寄生虫学会大会, Mar. 2009, 日本寄生虫学会, 順天堂大学、東京

  • ブラストシスチスの新規遺伝子型サブタイプ8と9を検出するプライマーの作製

    YOSHIKAWA Hisao

    第64回日本寄生虫学会西日本支部大会, Nov. 2008

  • 当院で経験したBlastocystis hominisの3例

    YOSHIKAWA Hisao

    第55回日本臨床検査医学会学術集会, Nov. 2008, 名古屋国際会議場

  • Inflammatory response in rats infected with Blastocystis hominis.

    YOSHIKAWA Hisao; Hisao Yoshikawa; Aya Iguchi; Minoru Yamada; Isao Kimata; Naoki Arizono

    16th Japanese-German Cooperative Symposium on Protozoan Diseases, Sep. 2008, Gottingen, Germany

  • Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu

    YOSHIKAWA Hisao; Hisao Yoshikawa; Zhiliang Wu; Kishor Pandy; Tetsuo Yanagi; Hiroji Kanbara

    The Tenth Asian-Pacific Congress for Parasitic Zoonoses, Aug. 2008, Taiwan

  • ブラストシスチス培養細胞と感染動物から得たシスト型の単一細胞からのSSU rRNA遺伝子配列の比較

    YOSHIKAWA Hisao

    第77回日本寄生虫学会大会, Apr. 2008

  • 有袋類のカンガルーとワラビーから分離されたブラストシスチス株の分子系統学的位置関係

    YOSHIKAWA Hisao

    第63回日本寄生虫学会西日本支部大会, Oct. 2007

  • ブラストシスチス栄養型とシスト型の単一細胞からのSSU rRNA遺伝子配列の比較

    YOSHIKAWA Hisao

    第63回日本寄生虫学会西日本支部大会, Oct. 2007, 日本寄生虫学会

  • ブラストシスチスの感染動物モデルの開発(10):感染ラットにおける抗体産生について

    YOSHIKAWA Hisao

    第63回日本寄生虫学会西日本支部大会, Oct. 2007

  • ブラストシスチスの感染動物モデルの開発(9):ラット由来RN94-9株の病原性について

    YOSHIKAWA Hisao

    第76回日本寄生虫学会大会, Mar. 2007

  • Recent advances on Blastocystis research

    YOSHIKAWA Hisao; Hisao Yoshikawa

    Nanjing Medical University, Nov. 2006, 南京医科大学 病原性微生物学教室, 南京医科大学

  • ブラストシスチスの分子系統的解析:哺乳類から昆虫類まで

    YOSHIKAWA Hisao

    第62回日本寄生虫学会西日本支部大会, Nov. 2006

  • 分類困難な寄生原虫の分子系統によるアプローチ

    YOSHIKAWA Hisao

    第62回日本寄生虫学会西日本支部大会, Nov. 2006, 愛知医科大学寄生虫学講座, 愛知県

  • Infectivity of human Blastocystis hominis isolates in chickens and rats.

    YOSHIKAWA Hisao; Hisao Yoshikawa; Aya Iguchi; Akiko Ebisu; Satiko Nagata; Yumie Saitou; Satoru Iwatani; Isao Kimata

    15th Japanese-German Cooperative Symposium on Protozoan Diseases, Sep. 2006

  • Phylogenetic study of Blastocystis isolates from mammals, birds, reptilians, amphibians and insects.

    YOSHIKAWA Hisao; H. Yoshikawa; Z. Wu; J. Howe; N. Geok-choo; KSW Tan

    XIth International Congress of Parasitology, Aug. 2006, England

  • Zoonotic potential and cyst passage of human Blastocystis hominis.

    YOSHIKAWA Hisao; Y. Saitou; A. Iguchi; S. Iwatani; I. Kimata; H. Yoshikawa

    The 9th Asian-Pasific Congress for Parasitic Zoonosis, Aug. 2006, Gifu, Japan

  • Infectivity and pathogenicity of Blastocystis.

    YOSHIKAWA Hisao; A. Iguchi; H. Yoshikawa; M. Yamada; I. Kimata; N. Arizono

    The 9th Asian-Pasific Congres for Parasitic Zoonosis, Aug. 2006, Gifu, Japan

  • Diversity and phylogeny in the genus Blastocystis

    YOSHIKAWA Hisao; Hisao Yoshikawa

    The 9th Asian-Pasific Congress for Parasitic Zoonosis, Aug. 2006, Prof. Dr. Yuzo Takahashi, Gifu University

  • ネパール、カトマンドゥの小児から分離したブラストシスチス株の遺伝子型と感染由来について

    YOSHIKAWA Hisao

    第75回日本寄生虫学会大会, May 2006

  • Zoonotic potential of human Blastocystis

    YOSHIKAWA Hisao; H. Yoshikawa

    National University of Singapore, Oct. 2005, Dr. KSW Tan, Singapore

  • Biology and molecular aspects on Blastocystis.

    YOSHIKAWA Hisao; H. Yoshikawa

    National Institute of Parasitic Diseases & Chinese Center for Disease Control, Shanghai, China, Sep. 2005, Dr. Zhou, China

  • Genomic polymorphism among Blastocystis isolates from human and animals.

    YOSHIKAWA Hisao; H. Yoshikawa

    Khon Kaen University, Thailand, Aug. 2005, Deapartment of Parasitology, Thailand

  • 寄生性原生生物:大家と住人の関係

    YOSHIKAWA Hisao

    平成17年度奈良女子大学大学院人間文化研究科 公開講座, Aug. 2005, 奈良女子大学大学院人間文化研究科, 奈良市

Association Memberships

  • International Society of Prototistologists

  • 日本顕微鏡学会

  • International Society of Protistologists

  • 日本寄生虫学会

  • 日独原虫病協会

Works

  • Molecular subtyping of human Blastocystis isolates in Thailand

    Sep. 2010

  • Molecular subtyping of human Blastocystis isolates in Thailand

    Sep. 2010

  • 腸管内感染原虫症の疫学研究

    Apr. 2005, Mar. - 2010

  • A survey of Blastocystis infection in humans and animals in Sumba Island, Indonesia

    Aug. 2009

  • A survey of Blastocystis infection in humans and animals in Sumba Island, Indonesia

    Aug. 2009

  • A survey of human Blastocystis in China

    Aug. 2005, Mar. - 2008

  • A survey of human Blastocystis in China

    Aug. 2005, Mar. - 2008

  • ブラストシスチスの病原性に関する研究

    2006, - 2008

  • A survey of Blastocystis hominis infection and genotype classification of B. hominis isolates in Thailand

    2005, - 2007

  • A survey of Blastocystis hominis infection and genotype classification of B. hominis isolates in Thailand

    2005, - 2007

  • Molecular phylogenetic study on human and animal Blastocystis in Turkey

    Aug. 2006

  • Molecular phylogenetic study on human and animal Blastocystis in Turkey

    Aug. 2006

  • 生物の初期進化の解明:研究の現状とデータ解析の問題点

    Apr. 2002, Mar. - 2003

  • クリプトスポリジウムの分子生物学的研究

    Apr. 2000, Mar. - 2003

  • Molecular biological studies on Cryptosporidium

    Apr. 2000, Mar. - 2003

  • 金属錯体界面活性剤から成るマイクロエマルジョン系による金属ナノ粒子の制御

    Apr. 2000, Mar. - 2002

  • A control of metal nanoparticles by microemulsion systems composed of metal complex surfactants

    Apr. 2000, Mar. - 2002

  • ミトコンドリアを持たない真核生物の系統的位置づけと真核生物の初期進化

    Apr. 1996, Mar. - 1999

  • 腸管内感染寄生原虫の分離と保存に関する研究

    1999

  • 腸管内寄生原虫ブラストシスチスの分子生物学的研究

    1996

  • Biological study on Blastocystis in humans and animals

    Sep. 1994

  • Biological study on Blastocystis in humans and animals

    Sep. 1994

Academic Contribution

  • 国際雑誌投稿論文の査読審査(4件), Review, 2019
  • 国際雑誌投稿論文の査読審査(5件), Review, 2018
  • 国際雑誌投稿論文の査読審査(6件), Review, 2017
  • 際雑誌投稿論文の査読審査(6件), Review, 2016
  • 1st International Blastocystis Symposium, Competition etc, Dr. Funda Dogruman-AI, 26 May 2015, 28 May 2015
  • 国際雑誌投稿論文の査読審査(3件), Review, 2015
  • 17th Japanese-German Cooperative Symposium on Protozoan Diseases & Workshop of Gastrointestinal Protozoan Diseases, Competition etc, 吉川尚男, 12 Sep. 2011, 16 Sep. 2011
  • 科学研究費の申請内容の審査, Review, National Medical Research Council (NMRC), 2011
  • Ph D学位の審査, Review, National University of Singapore, 2010

Others

  • 国際交流の推進(共同研究と外国人研究者の受け入れ), 1994, 9999, 国際共同研究(実施国名と共著論文数):アメリカ(2)、シンガポール(6)、トルコ共和国(4)、バングラデシュ人民共和国(1)、中華人民共和国(2)、ネパール連邦民主共和国(1)、タイ王国(1)、デンマーク(2)、インドネシア共和国(5) 長期外国人研究者の受け入れ・国名(期間と費用負担先):シンガポール(1994. 9.25-1994. 11.9. JSPS)(1997. 11. 24-1997. 12. 14. JSPS); トルコ共和国(2007. 6. 30-2007. 7. 28. Gazi University);中華人民共和国(2017. 9. 23-2017. 11. 30. 国立寄生虫病研究所)


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