Researchers Database

YOSHIKAWA Hisao

FacultyFaculty Division of Natural Sciences Research Group of Biological Sciences
PositionAssociate Professor
Last Updated :2022/10/06

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Profile and Settings

  • Name (Japanese)

    Yoshikawa
  • Name (Kana)

    Hisao

Degree

  • Doctor of Medical Science, Kyoto Prefectural University of Medicine
  • Master of Science, Konan University

Research Interests

  • protist, diversity, molecular phylogeny, Blastocystis

Research Experience

  • Apr. 2014, Mar. 2015, 金沢大学 非常勤講師
  • May 2013, May 2013, 京都府立医科大学・非常勤講師
  • Apr. 2012, Mar. 2013, 京都府立医科大学 非常勤講師
  • Apr. 2001, Mar. 2009, Gifu University, School of Medicine, \npart-time instructor
  • Apr. 1990, Mar. 2009, Kyoto Prefectural University of Medicine\npart-time instructor
  • Apr. 2007, 奈良女子大学 准教授
  • Sep. 1990, Aug. 1991, University of Oklahoma, Department of Microbiology and Immunology, Health Sciences Center, Visiting Professor
  • Sep. 1989, Mar. 1990, 京都大学医療技術短期大学部\n非常勤講師
  • Nov. 1989, Nara Women's University\nAssociate Professor
  • Apr. 1986, Oct. 1989, Kyoto Prefectural University of Medicine\nResearch Associate
  • Apr. 1988, Mar. 1989, Kyoto Women's University\npart-time instructor

Education

  • 1986, Kyoto Prefectural University of Medicine, Graduate School, Division of Medicine, 病理系
  • 1982, Konan University, Graduate School, Division of Science and Technology (Konan University), Biology
  • 1980, Konan University, Faculty of Science, 生物学

Association Memberships

  • International Society of Prototistologists, Jan. 1983, Dec. 2015
  • 日本顕微鏡学会, Apr. 1983, Dec. 2015
  • International Society of Protistologists, Jan. 1983, Dec. 2015
  • 日本寄生虫学会, Jan. 1983, 9999
  • 日独原虫病協会, Jan. 1985, Dec. 2019

Academic Contribution

  • 国際雑誌投稿論文の査読審査(4件), Review, 2019
  • 国際雑誌投稿論文の査読審査(5件), Review, 2018
  • 国際雑誌投稿論文の査読審査(6件), Review, 2017
  • 際雑誌投稿論文の査読審査(6件), Review, 2016
  • 1st International Blastocystis Symposium, Competition etc, Dr. Funda Dogruman-AI, 26 May 2015, 28 May 2015
  • 国際雑誌投稿論文の査読審査(3件), Review, 2015
  • 17th Japanese-German Cooperative Symposium on Protozoan Diseases & Workshop of Gastrointestinal Protozoan Diseases, Competition etc, 吉川尚男, 12 Sep. 2011, 16 Sep. 2011
  • 科学研究費の申請内容の審査, Review, National Medical Research Council (NMRC), 2011
  • Ph D学位の審査, Review, National University of Singapore, 2010
  • 国際雑誌投稿論文の査読審査(2件), Peer review etc, 2021
  • 国際雑誌投稿論文の査読審査(6件), Peer review etc, 01 Jan. 2020, 31 Dec. 2020

Others

  • 国際交流の推進(共同研究・共著論文数、および外国人研究者の受け入れ), 1994, 9999, 国際共同研究(実施国名と共著論文数):アメリカ(2)、シンガポール(6)、トルコ共和国(4)、バングラデシュ人民共和国(1)、中華人民共和国(2)、ネパール連邦民主共和国(1)、タイ王国(1)、デンマーク(2)、インドネシア共和国(5) 長期外国人研究者の受け入れ・国名(期間と費用負担先):シンガポール(1994. 9.25-1994. 11.9. JSPS)(1997. 11. 24-1997. 12. 14. JSPS); トルコ共和国(2007. 6. 30-2007. 7. 28. Gazi University);中華人民共和国(2017. 9. 23-2017. 11. 30. 国立寄生虫病研究所)

Ⅱ.研究活動実績

Published Papers

  • Refereed, Parasitology international, Three monophyletic clusters in Retortamonas species isolated from vertebrates., Joko Hendarto; Tetsushi Mizuno; Anggi P N Hidayati; Ismail E Rozi; Puji B S Asih; Din Syafruddin; Hisao Yoshikawa; Makoto Matsubayashi; Masaharu Tokoro, Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet been elucidated. Haplotypes of the 18S small subunit ribosomal RNA locus (1836-1899 bp) of Retortamonas spp. from humans (n = 8), pigs (n = 6), dogs (n = 1), goats (n = 16), water buffalos (n = 23), cattle (n = 7), rats (n = 3), and chickens (n = 5) were analyzed with references isolated from non-human mammals, amphibians, and insects. Phylogenetic and network analyses revealed a statistically supported three cluster formation among the vertebrate-isolated haplotypes, while insect-isolated haplotypes were independently clustered with Chilomastix. In the clade of vertebrate isolates, assemblage A (amphibian genotype), which included the amphibian references, was addressed as an out-group of the other clusters. Assemblage B (mammalian and chicken genotype) included most haplotypes from various mammals including humans with the haplotypes isolated from a chicken. Human isolates were all classified into this assemblage, thus assemblage B might correspond to R. intestinalis. Assemblage C (bovine genotype), which included specific haplotypes from water buffalos and cattle, was addressed as a sister lineage of assemblage B. Among the diversified haplotypes of assemblage B, a specific haplotype, which was identified from multiple host mammals (humans, dogs, pigs, cattle, water buffalos, elks, goats, and rats), indicates the potential zoonotic transmission of the Retortamonas among them. The genotyping classification of retortamonads could contribute to a better understanding of its molecular epidemiology, especially among humans and related host organisms., Apr. 2019, 69, 1, 93, 98, Scientific journal, True
  • Refereed, Tropical medicine and health, Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia., Takahiro Matsumura; Joko Hendarto; Tetsushi Mizuno; Din Syafruddin; Hisao Yoshikawa; Makoto Matsubayashi; Taro Nishimura; Masaharu Tokoro, Background: Although parasites are still endemic in developing areas, residents in those regions seem not to be affected by the presence of intestinal protozoans. This study aimed to investigate whether pathogenic and commensal protozoans are the causal agents of diarrhea via a school-based cross-sectional survey conducted in Indonesia, in September 2016. Results: Molecular screening for intestinal protozoans in collected 144 stool samples from healthy students (age range 7-15 years) was carried out. The prevalence of protozoan parasites was as follows: Giardia intestinalis (56.3%), Entamoeba histolytica (0%), E. dispar (6.9%), E. moshkovskii (0%), E. hartmanni (31.3%), and E. coli (44.4%). Observational evaluation of stool conditions using the Bristol stool chart confirmed the loose stool rate (33.3-90.9%) in each age group. Logistic regression analysis of protozoan infection or colonization for loose stool (mild to severe diarrhea) as an outcome revealed no significant findings in examined protozoans including pathogenic G. intestinalis infection [adjusted odds ratio (AOR) 0.78, 95% confidence interval (CI) 0.36-1.67], except in E. hartmanni colonization (AOR 2.81, 95% CI 1.1-3.7, P = 0.026). Conclusions: The molecular survey of intestinal protozoans targeting healthy population with their stool form evaluation could address the pathogenicity of those parasites appropriately. In comparatively higher-age children at least 7 years of age or greater in the endemic area, G. intestinalis could regard commensal, while E. hartmanni seems to possess a certain pathogenicity as a causal agent of mild diarrhea., 2019, 47, 7, 7, 7, Scientific journal, False
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Molecular phylogeny of Blastocystis isolates from wild rodents captured in Indonesia and Japan, Maai Katsumata; Hisao Yoshikawa; Masaharu Tokoro; Tetsushi Mizuno; Takehiro Nagamoto; Joko Hendarto; Puji B.S. Asih; Ismail E. Rozi; Isao Kimata; Kazutoshi Takami; Din Syafruddin, Blastocystis sp. is a common intestinal protist found worldwide in a variety of animals, including humans. Currently, 17 subtypes (STs) of Blastocystis isolates from mammalian and avian host species have been reported based on the small subunit ribosomal RNA gene (SSU rDNA). Among these, human Blastocystis were only identified among STs 1-9. Except ST9, all other STs comprised isolates from humans and other animal species. Entire sequence data of the SSU rDNA of nine Blastocystis isolates from laboratory rats or guinea pigs previously showed ST4, whereas Blastocystis isolates from wild rodents have not been addressed genetically. In this study, Blastocystis infection in wild rodents was surveyed in Indonesia and Japan, and 11 and 12 rodent Blastocystis parasites were obtained from Rattus exulans and R. novercious, respectively. All new Blastocystis isolates from wild rodents were identified as ST4 based on the SSU rDNA sequences. The best tree inferred with the entire sequences of the SSU rDNA of all ST4 isolates including 17 data registered in GenBank clearly showed monophyletic ST4A and ST4B clades. Although ST4 isolates from laboratory rats were separated into these two clades, all Blastocystis isolates from wild rodents in the present study were positioned into the clade ST4A and further separated into two sub-clusters within the clade ST4A according to the location of the host species. Considering the fact that laboratory rats were susceptible to both ST4A and ST4B, separation of the monophyletic sub-clusters of Blastocystis isolates from Indonesian Polynesian rats and Japanese brown rats may indicate the presence of geographical variations rather than a host-specific separation. In either way, the robust host preference to rodent species of ST4 Blastocystis was also confirmed., Sep. 2018, 117, 9, 2841, 2846, Scientific journal, True
  • Refereed, Parasitology International, Elsevier BV, Human Blastocystis subtyping with subtype-specific primers developed from unique sequences of the SSU rRNA gene, Hisao Yoshikawa; Ayana Iwamasa, Dec. 2016, 65, 6, 785, 791, Scientific journal
  • Refereed, Parasitology International, Elsevier BV, Molecular survey of Blastocystis sp. from humans and associated animals in an Indonesian community with poor hygiene, Hisao Yoshikawa; Masaharu Tokoro; Takehiro Nagamoto; Shunsuke Arayama; Puji B.S. Asih; Ismail E. Rozi; Din Syafruddin, Blastocystis sp. is a common parasite found in human and animal fecal samples. Currently, human Blastocystis isolates are classified into nine subtypes (STs) based on the phylogeny of their small subunit ribosomal RNA genes (SSU rDNAs). Since eight of the nine STs, except for ST9, have been reported in both humans and animals, these parasites are considered to be potentially zoonotic STs. To evaluate whether zoonotic transmissions play a main role in the lifecycle of Blastocystis, STs derived from humans, domestic pigs, domestic chickens, and wild rodents in a community with poor hygiene in Sumba Island, Indonesia were surveyed. Although fecal cross-contaminations between humans and animals were likely common at the investigation site, the confirmed major Blastocystis STs, which were detected as intense bands on gels following PCR targeting of the SSU rDNA, were different in each host species. STs 1-3 were found in resident children, while ST5, ST7, and ST4 were found in domestic pigs and chickens, and in wild rodents, respectively. Faint bands of STs 1, 2, and 7 were detected in samples from pigs, while no minor STs were observed in samples from the other host species. The distinct distributions of the major STs among the host animals examined, including humans, indicate host specificity in the lifecycle of Blastocystis. Considering the coprophagous nature of pigs, the presence of minor STs observed only in pigs could be explained by the mechanical passage of contaminated fecal materials., Dec. 2016, 65, 6, 780, 784, Scientific journal, True
  • Refereed, PARASITOLOGY INTERNATIONAL, ELSEVIER IRELAND LTD, Blastocystis phylogeny among various isolates from humans to insects, Hisao Yoshikawa; Yukiko Koyama; Erika Tsuchiya; Kazutoshi Takami, Blastocystis is a common unicellular eukaryotic parasite found not only in humans, but also in various kinds of animal species worldwide. Since Blastocystis isolates are morphologically indistinguishable, many molecular biological approaches have been applied to classify these isolates. The complete or partial sequences of the small subunit rRNA gene (SSU rDNA) are mainly used for comparisons and phylogenetic analyses among Blastocystis isolates. However, various lengths of the partial SSU rDNA sequence have been used for phylogenetic inference among genetically different isolates. Based on the complete SSU rDNA sequences, consensus terminology of nine subtypes (STs) of Blastocystis sp. that were supported by phylogenetically monophyletic nine clades was proposed in 2007. Thereafter, eight additional kinds of STs comprising non-human mammalian Blastocystis isolates have been reported based on the phylogeny of SSU rDNA sequences, while STs 11 and 12 were only proposed on the base of partial sequences. Although many sequence data from mammalian and avian Blastocystis are registered in GenBank, only limited data on SSU rDNA are available for poikilotherm-derived Blastocystis isolates. Therefore, the phylogenetic positions of the reptilian/amphibian Blastocystis clades are unstable. The phylogenetic inference of various STs comprising mammalian and/or avian Blastocystis isolates was verified herein based on comparisons between partial and complete SSU rDNA sequences, and the phylogenetic positions of reptilian and amphibian Blastocystis isolates were also investigated using 14 new Blastocystis isolates from reptiles with all known isolates from other reptilians, amphibians, and insects registered in GenBank. (C) 2016 Published by Elsevier Ireland Ltd., Dec. 2016, 65, 6, 750, 759, Scientific journal
  • Refereed, KOREAN JOURNAL OF PARASITOLOGY, KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI, Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia, Mudyawati Kamaruddin; Masaharu Tokoro; Md. Moshiur Rahman; Shunsuke Arayama; Anggi P. N. Hidayati; Din Syafruddin; Puji B. S. Asih; Hisao Yoshikawa; Ei Kawahara, Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mills, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species., Oct. 2014, 52, 5, 471, 478, Scientific journal
  • Refereed, PROTIST, ELSEVIER GMBH, URBAN & FISCHER VERLAG, Genetic Diversity of Blastocystis in Livestock and Zoo Animals, Mohammed A. Alfellani; Derya Taner-Mulla; Alison S. Jacob; Christine Atim Imeede; Hisao Yoshikawa; C. Rune Stensvold; C. Graham Clark, Blastocystis is a common unicellular anaerobic eukaryote that inhabits the large intestine of many animals worldwide, including humans. The finding of Blastocystis in faeces in mammals and birds has led to proposals of zoonotic potential and that these hosts may be the source of many human infections. Blastocystis is, however, a genetically diverse complex of many distinct organisms (termed subtypes; STs), and sampling to date has been limited, both geographically and in the range of hosts studied. In order to expand our understanding of host specificity of Blastocystis STs, 557 samples were examined from various non-primate animal hosts and from a variety of different countries in Africa, Asia and Europe. STs were identified using 'barcoding' of the small subunit rRNA gene using DNA extracted either from culture or directly from faeces. The host and geographic range of several STs has thereby been greatly expanded and the evidence suggests that livestock is not a major contributor to human infection. Two new STs were detected among the barcode sequences obtained; for these, and for three others where the data were incomplete, the corresponding genes were fully sequenced and phylogenetic analysis was undertaken. (c) 2013 Elsevier GmbH. All rights reserved., Jul. 2013, 164, 4, 497, 509, Scientific journal
  • Refereed, Parasitology Research, Subtype identification of Blastocystis spp. isolated from patients in a major hospital in northeastern Thailand, Sarinee Jantermtor; Porntip Pinlaor; Kookwan Sawadpanich; Somchai Pinlaor; Arunnee Sangka; Chotechana Wilailuckana; Wachanan Wongsena; Hisao Yoshikawa, The present study is aimed to identify the prevalence of Blastocystis subtypes isolated from patients in a major hospital in northeastern Thailand. A total of 562 stool samples were examined by culture technique, and 56 Blastocystis-positive samples were analyzed further by the combination of restriction fragment length polymorphism (RFLP) followed by polymerase chain reaction with sequence-tagged site primers (PCR-STS). By RFLP profiles, Blastocystis genotypes were categorized into four groups: group A (12, 21.4 %), group B (32, 57.1 %), group C (10, 17.9 %), and group D (2, 3.6 %). By PCR-STS, only four subtypes were identified. All 12 (21.4 %) isolates in group A were identified as subtype 1. Similarly, all 32 (57.1 %) isolates in group B were subtype 3. In group C, 10 (17.9 %) samples were all subtype 7, and two samples (3.6 %) in group D were both subtype 6. Of 56 Blastocystis-positive patients, 31 (55.4 %) were asymptomatic and 22 (39.4 %) have gastrointestinal symptoms. No significant association was observed between the Blastocystis subtypes and the clinical features. Among the Blastocystis-positive patients, the most characteristic stool samples were loose (78.6 %) and soft (17.9 %). In conclusion, the most common Blastocystis spp. in northeastern Thailand was subtype 3 followed by subtype 1. Relatively minor subtypes, subtype 6 and subtype 7 which are considered as avian subtypes, were found for the first time in humans in Thailand. © 2012 Springer-Verlag Berlin Heidelberg., Apr. 2013, 112, 4, 1781, 1786, Scientific journal
  • Refereed, Blastocystis:Pathogen or Passenger?, Morphology of human and animal Blastocystis isolates with special reference to reproductive modes., YOSHIKAWA Hisao; Minoru Yamamada; Hisao Yoshikawa, Dec. 2012, 9-35
  • Refereed, Blastocystis:Pathogen or Passenger?, Epidemiology, transmission, and zoonotic potential of Blastocystis in human and animals, YOSHIKAWA Hisao, Dec. 2012, 37-49
  • Refereed, Blastocystis:Pathogen or Passenger?, Molecular approaches on systematic position, genetic polymorphism, and classification of Blastocystis from humans and animals., YOSHIKAWA Hisao, Dec. 2012, 85-94
  • Refereed, Blastocystis: Pathogen or Passenger?, Landmarks in the Discovery of Blastocystis Stages, YOSHIKAWA Hisao; H. Mehlhorn; M. Yamada; H. Yoshikawa, Dec. 2012, 1-8
  • Not Refereed, INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, ELSEVIER SCI LTD, A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia, M. Tokoro; H. Yoshikawa; D. Syafruddin, Jun. 2012, 16, E177, E177
  • Refereed, Clinical Parasitology, 赤痢アメーバ感染症の2例:Entamoeba disper感染にヒトブラストシスチスおよび小型アメーバが検出されたブラジル人症例と回腸部に潰瘍を認めた無症候性E. histolytica感染の日本人症例, YOSHIKAWA Hisao, 2012, 23, 1, 82-85
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples, Hisao Yoshikawa; Funda Dogruman-AI; Songul Turk; Semra Kustimur; Neriman Balaban; Nedim Sultan, Oct. 2011, 109, 4, 1045, 1050, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, PCR-based subtyping of Blastocystis isolates from symptomatic and asymptomatic individuals in a major hospital in Ankara, Turkey, Funda Dogruman-Al; Hisao Yoshikawa; Semra Kustimur; Neriman Balaban, Dec. 2009, 106, 1, 263, 268, Scientific journal
  • Refereed, MEMORIAS DO INSTITUTO OSWALDO CRUZ, FUNDACO OSWALDO CRUZ, Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey, Funda Dogruman-Al; Semra Kustimur; Hisao Yoshikawa; Candan Tuncer; Zahide Simsek; Mehmet Tanyuksel; Engin Araz; Kenneth Boorom, Blastocystis infection has been reported to be associated with irritable bowel syndrome (IBS), inflammatory bowel disease (IBD) and chronic diarrhoea. The availability of data on the subtypes of Blastocystis found in these patient groups would be of interest in understanding the significance of Blastocystis infection in chronic illness. In this study, we identify Blastocystis subtypes found in patients presenting with IBS, IBD, chronic diarrhoea and asymptomatic patients in Ankara, Turkey. Blastocystis was detected in 11 symptomatic patients by microscopy and 19 by stool culture. Stool culture was more sensitive than microscopy in identifying Blastocystis. Using standard nomenclature adopted in 2007, Blastocystis sp. subtype 3 was the most common in all groups, followed by Blastocystis sp. subtype 2. Identical subtypes of Blastocystis are found in patients with IBS, IBD and chronic diarrhoea. These particular subtypes show low host specificity and are carried by humans and some farm animals. The subtypes of Blastocystis that are commonly found in rodents and certain wild birds were not found in these patients. We suggest a model in which the severity of enteric protozoan infection may be mediated by host factors., Aug. 2009, 104, 5, 724, 727, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Expression of interferon gamma and proinflammatory cytokines in the cecal mucosa of rats experimentally infected with Blastocystis sp. strain RN94-9, Aya Iguchi; Hisao Yoshikawa; Minoru Yamada; Isao Kimata; Naoki Arizono, Jun. 2009, 105, 1, 135, 140, Scientific journal
  • Refereed, Veterinary Parasitology, Elsevier BV, Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal, Hisao Yoshikawa; Zhiliang Wu; Kishor Pandey; Basu Dev Pandey; Jeevan Bahadur Sherchand; Tetsuo Yanagi; Hiroji Kanbara, Mar. 2009, 160, 3-4, 295, 300, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis, Funda Dogruman-Al; Hande Dagci; Hisao Yoshikawa; Özgur Kurt; Mete Demirel, Aug. 2008, 103, 3, 685, 689, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore, Kenneth H. S. Wong; G. C. Ng; Raymond T. P. Lin; H. Yoshikawa; Mark B. Taylor; Kevin S. W. Tan, Mar. 2008, 102, 4, 663, 670, Scientific journal
  • Refereed, Parasitology International, Elsevier BV, Molecular epidemiology of human Blastocystis in a village in Yunnan province, China, Lan-Hua Li; Xiao-Nong Zhou; Zun-Wei Du; Xue-Zhong Wang; Li-Bo Wang; Jin-Yong Jiang; Hisao Yoshikawa; Peter Steinmann; Jürg Utzinger; Zhiliang Wu; Jia-Xu Chen; Shao-Hong Chen; Ling Zhang, Dec. 2007, 56, 4, 281, 286, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China, Lan Hua Li; Xiao Ping Zhang; Shan Lv; Ling Zhang; Hisao Yoshikawa; Zhiliang Wu; Peter Steinmann; Jürg Utzinger; Xiao Mei Tong; Shao Hong Chen; Xiao Nong Zhou, 18 Oct. 2007, 102, 1, 83, 90, Scientific journal
  • Refereed, Parasitology International, Elsevier BV, Infectivity of different genotypes of human Blastocystis hominis isolates in chickens and rats, Aya Iguchi; Akiko Ebisu; Satiko Nagata; Yumie Saitou; Hisao Yoshikawa; Satoru Iwatani; Isao Kimata, Jun. 2007, 56, 2, 107, 112, Scientific journal
  • Refereed, Trends in Parasitology, Elsevier BV, Terminology for Blastocystis subtypes – a consensus, C. Rune Stensvold; G. Kumar Suresh; Kevin S.W. Tan; R.C. Andrew Thompson; Rebecca J. Traub; Eric Viscogliosi; Hisao Yoshikawa; C. Graham Clark, Mar. 2007, 23, 3, 93, 96, Scientific journal
  • Refereed, The Journal of Eukaryotic Microbiology, Wiley, Ultrastructural and Phylogenetic Studies on Blastocystis Isolates from Cockroaches, HISAO YOSHIKAWA; ZHILIANG WU; JOSEPHINE HOWE; TETSUO HASHIMOTO; NG GEOK-CHOO; KEVIN S. W. TAN, Jan. 2007, 54, 1, 33, 37, Scientific journal
  • Refereed, Parasitic Zoonosis in Asian-Pacific Regions 2006, Zoonotic potential and cyst passage of human Blastocystis hominis., YOSHIKAWA Hisao; Saitou Y, Dec. 2006, 20-24
  • Refereed, Parasitic Zoonosis in Asian-Pacific Regions 2006, Infectivity and pathogenicity of Blastocystis., YOSHIKAWA Hisao; Iguchi A, Dec. 2006, 25-31
  • Refereed, 岐阜市民病院年報, アメーバ性大腸炎にBlastocystis hominisとGiardia lambliaの同時感染を認めた一症例, YOSHIKAWA Hisao, 2006, 26, 47-51
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Infectivity of Blastocystis isolates from chickens, quails and geese in chickens, Aya Tanizaki; Hisao Yoshikawa; Satoru Iwatani; Isao Kimata, Apr. 2005, 96, 1, 57, 61, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Fecal-oral transmission of the cyst form of Blastocystis hominis in rats, Hisao Yoshikawa; Kumi Yoshida; Ayumi Nakajima; Kimie Yamanari; Satoru Iwatani; Isao Kimata, Dec. 2004, 94, 6, 391, 396, Scientific journal
  • Refereed, Trends in Parasitology, Elsevier BV, Problems in speciation in the genus Blastocystis, Hisao Yoshikawa; Keiko Morimoto; Zhiliang Wu; Mulkit Singh; Tetsuo Hashimoto, Jun. 2004, 20, 6, 251, 255, Scientific journal
  • Refereed, Veterinary Parasitology, Elsevier BV, A survey of Blastocystis infection in anuran and urodele amphibians, Hisao Yoshikawa; Keiko Morimoto; Minako Nagashima; Naoko Miyamoto, Jun. 2004, 122, 2, 91, 102, Scientific journal
  • Refereed, Microbiology, Microbiology Society, PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds, Hisao Yoshikawa; Niichiro Abe; Zhiliang Wu, 01 May 2004, 150, 5, 1147, 1151, Scientific journal
  • Refereed, 顕微鏡, クリプトスポリジウムの特異な細胞内寄生様式について, YOSHIKAWA Hisao, Mar. 2004, 39, 1, 48-52
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries, Hisao Yoshikawa; Zhiliang Wu; Isao Kimata; Motohiro Iseki; Ibne Karim; M D Ali; Momammad B Hossain; Viqar Zaman; Rashidul Haque; Yuzo Takahashi, 01 Jan. 2004, 92, 1, 22, 29, Scientific journal
  • Refereed, The Journal of Eukaryotic Microbiology, Wiley, Genomic Polymorphism Among Blastocystis Isolates and Development of PCR-Based Identification of Zoonotic Isolates, HISAO YOSHIKAWA; NIICHIRO ABE; ZHILIANG WU, Jul. 2003, 50, s1, 710, 711, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Zoonotic genotypes of Blastocystis hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene, Niichiro Abe; Zhiliang Wu; Hisao Yoshikawa, Jun. 2003, 90, 2, 124, 128, Scientific journal
  • Refereed, Veterinary Parasitology, Elsevier BV, Molecular characterization of Blastocystis isolates from primates, Niichiro Abe; Zhiliang Wu; Hisao Yoshikawa, May 2003, 113, 3-4, 321, 325, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Molecular characterization of Blastocystis isolates from birds by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene, Niichiro Abe; Zhiliang Wu; Hisao Yoshikawa, Mar. 2003, 89, 5, 393, 396, Scientific journal
  • Refereed, The Journal of Eukaryotic Microbiology, Wiley, Freeze-Fracture and Cytoehemical Studies on the in Vitro Cyst Form of Reptilian Blastocystis pythoni, HISAO YOSHIKAWA; MINAKO NAGASHIMA; KEIKO MORIMOTO; YASUKO YAMANOUTI; EU HIAN YAP; MULKIT SINGH, Jan. 2003, 50, 1, 70, 75, Scientific journal
  • Refereed, Journal of Parasitology, American Society of Parasitologists, Molecular comparative studies among Blastocystis isolates obtained from humans and animals, Hisao Yoshikawa; Zhiliang Wu; Isao Nagano; Yuzo Takahashi, This study compared specific polymerase chain reaction (PCR) primers and phylogenetic tree analysis of restriction fragment length polymorphism using the small subunit ribosomal RNA gene in various Blastocystis populations. A phylogenetic tree was constructed using 12 restriction enzymes and a sample pool of 22 isolates, including 2 reference strains and Proteromonas lacertae as an outgroup. The analysis showed that the 22 isolates could be separated into 7 clusters. Four of the 7 clusters were mixed groups that comprised isolates from both humans and nonhuman hosts. The other 3 clusters contained isolates from humans or nonhuman hosts only. The phylogenetic analysis also showed that B. hominis isolates from geographical separated areas did not necessarily cluster in the genetically different groups. The results of genetic homology and phylogenetic tree analysis among Blastocystis isolates from humans and animals indicated that all isolates from animals appear to be B. hominis. Polymerase chain reaction amplifications using previously described and newly defined specific primers mirrored the clusters obtained by the phylogenetic tree analysis. Our results show that primer PCR can be used as a powerful tool for the typing of Blastocystis populations., 2003, 89, 3, 585, 594, Scientific journal
  • Refereed, Parasitology, Cambridge University Press (CUP), Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates, N. ARISUE; T. HASHIMOTO; H. YOSHIKAWA, Genes encoding small subunit ribosomal RNA (SSUrRNA) of 16 Blastocystis isolates from humans and other animals were amplified by the polymerase chain reaction, and the corresponding fragments were cloned and sequenced. Alignment of these sequences with the previously reported ones indicated the presence of 7 different sequence patterns in the highly variable regions of the small subunit ribosomal RNA. Phylogenetic reconstruction analysis using Proteromonas lacertae as the outgroup clearly demonstrated that the 7 groups with the different sequence patterns are separated to form independent clades, 5 of which consisted of the Blastocystis isolates from both humans (B. hominis) and other animals. The presence of 3 higher order clades was also clearly supported in the phylogenetic tree. However, a relationship among the 4 groups including these 3 higher order clades was not settled with statistical confidence. The remarkable heterogeneity of small subunit ribosomal RNAs among different Blastocystis isolates found in this study confirmed, with sequence-based evidence, that these organisms are genetically highly divergent in spite of their morphological identity. The highly variable small subunit ribosomal RNA regions among the distinct groups will provide useful information for the development of group-specific diagnostic primers., Jan. 2003, 126, 1, 1, 9, Scientific journal
  • Refereed, CHEMISTRY LETTERS, CHEMICAL SOC JAPAN, Formation of palladium(0) nanoparticles from a microemulsion system composed of bis (N-octylethylenediamine)palladium(II) chloride complex, M Iida; S Ohkawa; H Er; N Asaoka; H Yoshikawa, Palladium(0) nanoparticles were obtained in high densities from a novel type reverse micelle (or W/O microemulsion) of bis(N-octylethylenediamine)palladium(II) chloride (=[Pd(octen)(2)]Cl-2)/water/chloroform (= CHCl3). The shape of the nanoparticles was largely affected by the phase of the additive, sodium boron hydride (= NaBH4) reductant, and by the replacement of a small amount of water with methanol., Oct. 2002, 10, 10, 1050, 1051, Scientific journal
  • Refereed, Veterinary Parasitology, Elsevier BV, A survey of Blastocystis sp. in livestock, pets, and zoo animals in Japan, Niichiro Abe; Mizuho Nagoshi; Kazutoshi Takami; Yoshinori Sawano; Hisao Yoshikawa, Jun. 2002, 106, 3, 203, 212, Scientific journal
  • Refereed, The Journal of Eukaryotic Microbiology, Wiley, Phylogenetic Position of Blastocystis hominis and of Stramenopiles Inferred from Multiple Molecular Sequence Data, NOBUKO ARISUE; TETSUO HASHIMOTO; HISAO YOSHIKAWA; YOSHIfflRO NAKAMURA; GEN NAKAMURA; FUMINORI NAKAMURA; TAKA-AKI YANO; MASAMI HASEGAWA, Jan. 2002, 49, 1, 42, 53, Scientific journal
  • Refereed, Journal of Clinical Microbiology, American Society for Microbiology, Genomic Analysis of Blastocystis hominisStrains Isolated from Two Long-Term Health Care Facilities, Hisao Yoshikawa; Niichiro Abe; Mizue Iwasawa; Syoko Kitano; Isao Nagano; Zhiliang Wu; Yuzo Takahashi, The genotype Blastocystis hominis is highly polymorphic. Therefore, a genetic marker would be a powerful tool for the identification or classification of B. hominis subtypes and could be used as a means to resolve the transmission route or origin of the parasite. To this end, 32 B. hominis isolates were collected from patients and/or staff members of two long-term health care facilities (facilities A and B), and these organisms were subjected to genotype analysis based on diagnostic PCR primers and restriction fragment length polymorphism (RFLP) of small subunit rRNA gene (rDNA). Based on PCR amplification using diagnostic primers which were developed from randomly amplified polymorphic DNA analysis of known strains of B. hominis, the 32 isolates of B. hominis were classified into three different subtypes. Thirty isolates, including twenty-four that were isolated from patients and a staff member, from facility A and all isolates isolated from six patients from facility B showed the same genotype. Two of six patients of facility B had been transferred from facility A, and these two patients also had the same-genotype B. hominis that corresponded to 24 isolates from facility A. This genotype strain may have been transmitted by these two patients from facility A to facility B, suggesting human-to-human transmission. In contrast, 2 of 26 isolates from facility A showed distinct genotypes, suggesting that the colonization by these two isolates is attributable to another infectious route. These different subtypes were subjected to RFLP analysis, and the RFLP profiles were correlated with the results obtained by diagnostic PCR primers. This study presents the first molecular evidence of possible human-to-human B. hominisinfection between and/or among two small communities., 2000, 38, 4, 1324, 1330, Scientific journal
  • Refereed, Molecular and Cellular Probes, Elsevier BV, Genomic polymorphism amongBlastocystis hominisstrains and development of subtype-specific diagnostic primers, Hisao Yoshikawa; Isao Nagano; Zhiliang Wu; Eu Hian Yap; Mulkit Singh; Yuzo Takahashi, Jun. 1998, 12, 3, 153, 159, Scientific journal
  • Refereed, Journal of Protozoology, Freeze-fracture studies of Cryptosporidium muris., YOSHIKAWA Hisao; H. Yoshikawa; M. Iseki, 1998, 38, 6, 171S-172S
  • Refereed, ICOPA IX - 9TH INTERNATIONAL CONGRESS OF PARASITOLOGY, MEDIMOND S R L, Isolation and characterization of Blastocystis spp. isolated from anuran amphibians, H Yoshikawa; K Morimoto; M Nagashima, Blastocystis spp. were isolated from three species of anuran amphibians in Japan. Eleven strains of Blastocystis sp were isolated from 13 frogs of Rana catesbeiana. Four strains of Blastocystis sp. were isolated from 21 frogs of Rana nigromaculata. All of 5 frogs of Bufo japonicus japonicus were positive of Blastocystis sp. Culturing of the organisms at various temperatures and temperature tolerance assays indicated that two strains isolated from a R. nigromaculata and a B. japonicus japonicus were different from Blastocystis hominis and Blastocystis lapemi isolated from a reptile. Based on the difference of temperature tolerance, two strains isolated from frogs might be new species of Blastocystis., 1998, 1075, 1079, International conference proceedings
  • Refereed, ELECTRON MICROSCOPY 1998, VOL 4, IOP PUBLISHING LTD, Cyst form of Blastocystis pythoni in vitro culture, H Yoshikawa; M Nagashima; K Morimoto, 1998, 403, 404, International conference proceedings
  • Refereed, The Journal of Eukaryotic Microbiology, Wiley, Heterogeneous Distribution of Membrane Cholesterol at the Attachment Site of Cryptosporidium muris to Host Cells, HISAO YOSHIKAWA; ISAO KIMATA; MOTOHIRO ISEKI, Sep. 1997, 44, 5, 454, 456, Scientific journal
  • Refereed, PROTOPLASMA, SPRINGER WIEN, Ultrastructural localization of basic proteins of Blastocystis hominis, H Yoshikawa; K Oishi, Basic proteins of Blastocystis hominis were detected by the ammoniacal silver and ethanolic phosphotungstic acid techniques using electron microscopy. The central vacuole showed many silver grains when treated with ammoniacal silver and an increased electron density when treated with phosphotungstic acid. The intensity of positive reactions correlated with the electron density of the central vacuole, because cells having an electron-lucent central vacuole showed no silver grain deposits. Since it is known that the concentration of electron-dense materials in the central vacuole increases during log phase of growth, and then decreases in stationary phase, this organelle must accumulate basic proteins during cell growth., 1997, 200, 1-2, 31, 34, Scientific journal
  • Refereed, International Journal for Parasitology, Elsevier BV, Freeze-fracture Cytochemistry of Membrane Cholesterol in Blastocystis hominis, H. Yoshikawa; A. Hayakawa, Oct. 1996, 26, 10, 1111, 1114, Scientific journal
  • Refereed, Molecular and Biochemical Parasitology, Elsevier BV, Phylogenetic position of Blastocystis hominis that contains cytochrome-free mitochondria, inferred from the protein phylogeny of elongation factor 1α, Yoshihiro Nakamura; Tetsuo Hashimoto; Hisao Yoshikawa; Takashi Kamaishi; Fuminori Nakamura; Ken-ichi Okamoto; Masami Hasegawa, May 1996, 77, 2, 241, 245, Scientific journal
  • Refereed, Journal of Eukaryotic Microbiology, Wiley, DNA Polymorphism Revealed by Arbitrary Primers Polymerase Chain Reaction AmongBlastocystisStrains Isolated from Humans, a Chicken, and a Reptile, HISAO YOSHIKAWA; ISAO NAGONO; EU HIAN YAP; MULKIT SINGH; YUZO TAKAHASHI, Mar. 1996, 43, 2, 127, 130, Scientific journal
  • Refereed, PROTOPLASMA, SPRINGER WIEN, Morphological changes in the central vacuole of Blastocystis hominis during in vitro culture, H Yoshikawa; A Hayakawa, Morphological changes in the central vacuole during the growth in in vitro culture of Blastocystis hominis were investigated by light and electron microscopy. Most cells in log phase and an early stationary phase showed a positive staining reaction in the central vacuole with PAS or Sudan black B stain, whereas cells in late stationary phase showed few positive reactions. Electron microscopic observations revealed that 95% of B. hominis cells in log phase and 50% of cells in early stationary phase, had a substantial accumulation of electron-dense material in the central vacuole. In contrast, only 25% of the organisms in late stationary phase had an electron-dense central vacuole, while more than 50% of cells had an electron-lucent central vacuole. These results indicate that B. hominis accumulated carbohydrates and lipids in the central vacuole during cell growth and that the organism probably consumed these metabolic substances during stationary growth. Therefore, it is strongly suggested that the central vacuole is an important organelle for storage of metabolic substances, such as carbohydrates and lipids, required for cell growth., 1996, 194, 1-2, 63, 68, Scientific journal
  • Refereed, JOURNAL OF PROTOZOOLOGY RESEARCH, NATL RESEARCH CENTER PROTOZOAN DISEASES, Antigenic differences between Blastocystis hominis and Blastocystis sp revealed by polyclonal and monoclonal, H Yoshikawa; Katagiri, I; XH Li; M BeckerHapak; DC Graves, The antigenic differences between Blastocystis hominis and Blastocystis sp. (isolated from a human and a chicken sources, respectively) were investigated by using polyclonal and monoclonal antibodies, Polyclonal antibodies did not reveal distinct antigenic differences between the strains by Western blot analysis, whereas monoclonal antibodies (MAbs) produced against B. hominis did detect antigenic polymorphism between B. hominis and Blastocystis sp, Six out of 8 MAbs were specific to B. hominis antigens and 2 MAbs reacted with both B. hominis and Blastocystis sp, antigens, Immunoelectron microscopy using these MAbs, revealed labeling on the filamentous layer or the central vacuole, Interestingly, the 6 MAbs reacted only with B. hominis antigens on the filamentous layer, whereas the other 2 MAbs labeled on the central vacuole, Therefore, the antigenic components of the filamentous layer may be strain or species specific. In contrast, the antigenic components of the central vacuole are shared by both B. hominis and Blastocystis sp, suggesting the metabolic substances in the central vacuole are antigenically common., Jul. 1995, 5, 3, 118, 128, Scientific journal
  • Refereed, JOURNAL OF ELECTRON MICROSCOPY, JAPANESE SOC ELECTRON MICRO BUS CENTER ACAD SOC JAPAN, LIGHT AND ELECTRON-MICROSCOPIC LOCALIZATION OF LIPIDS IN BLASTOCYSTIS-HOMINIS, H YOSHIKAWA; J SATOH; Y ENOSE, Localization of the lipid of Blastocystis hominis was histochemically demonstrated, B. hominis was fixed with glutaraldehyde, treated with detergents, and stained with Sudan black B or Nile blue. The central vacuole of B. hominis stained with Sudan black B showed many black droplets with great variation in staining intensity. In Nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. These results indicated the presence of neutral lipids and/or acidic lipids in the central vacuole, At the ultrastructural level, the central vacuole and some cytoplasmic vesicles showed enhanced electron density after post-fixation with imidazole-buffered osmium tetroxide solution, Great variation in the density and distribution of the electron-dense materials was observed in the central vacuole. Since the electron-dense vesicles were frequently observed in the cytoplasm, B. hominis may accumulate the lipid in the central vacuole, the function of which is not well known., Apr. 1995, 44, 2, 100, 103, Scientific journal
  • Refereed, The Journal of Eukaryotic Microbiology, Wiley, Histochemical Detection of Carbohydrates of Blastocystis hominis, HISAO YOSHIKAWA; NOBUKO KUWAYAMA; YOSHIMI ENOSE, Jan. 1995, 42, 1, 70, 74, Scientific journal
  • Refereed, JOURNAL OF ELECTRON MICROSCOPY, OXFORD UNIV PRESS UNITED KINGDOM, DISTRIBUTION OF FILIPIN-STEROL COMPLEXES IN THE BLOOD-STREAM FORM OF TRYPANOSOMA-BRUCEI-GAMBIENSE, H YOSHIKAWA; J FURUKI; Y TAKAHASHI; H MORIOKA; Y YOSHIDA, The polyene antibiotic, filipin, was used as a probe for demonstrating sterols in the plasma membrane of Trypanosoma brucei gambiense. Three different regions of the continuous plasma membrane, over the cell body proper, flagellar pocket, and flagellum, were compared as to density and distribution of the filipin-sterol complexes by freeze-fracture method. The density of the complexes was highest in the cell body membrane and lowest over the flagellar pocket. The filipin-sterol complexes in the cell body membrane were distributed homogeneously on both the protoplasmic and exoplasmic faces except in the zone of flagellar attachment. This junctional zone showed a linear, complex-poor region. In the flagellar membranes, a line of complex-poor regions was observed along the juncture of the flagellum to the cell body. At the neck of the flagellar pocket, the membranes of the flagellar pocket and flagellum were closely opposed, with few filipin-sterol complexes on either membrane. At the base of the flagellar shaft, the complexes were completely lacking on both faces. Based on these observations, the zones of juncture observed in T. b. gambiense seems to be similar in ultrastructure to mammalian cell junctions. such as tight, gap, septate junctions and desmosomes, which show a nearly complete absence of the filipin-sterol complexes., Oct. 1992, 41, 5, 364, 368, Scientific journal
  • Refereed, The Journal of Protozoology, Wiley, Freeze-Fracture Study of the Site of Attachment ofCryptosporidium murisin Gastric Glands, HISAO YOSHIKAWA; MOTOHIRO ISEKI, Sep. 1992, 39, 5, 539, 544, Scientific journal
  • Refereed, JOURNAL OF PROTOZOOLOGY, SOC PROTOZOOLOGISTS, FREEZE-FRACTURE STUDIES OF CRYPTOSPORIDIUM-MURIS, H YOSHIKAWA; M ISEKI, The attachment site of Cryptosporidium muris to host cells was investigated using the freeze-fracture method. Cryptosporidium muris was enveloped by a double membrane of host plasma membrane origin, which formed the parasitophorous vacuole. The outer membrane of the double membrane was continuous with the host plasma membrane at the dense band, while the inner membrane was connected with the anterior part of the parasite plasma membrane at the annular ring. The density of intramembranous particles (IMP) was dramatically altered at the above two junctures. The outer parasitophorous membrane showed low IMP-density as compared to the host plasma membrane, although both membranes were continuous. The inner parasitophorous membrane had few IMP, whereas the parasite plasma membrane showed numerous IMP. When the attachment sites of parasites and host cells were fractured, circular-shaped fractured faces were observed on both sites of the parasite and host cell. These exposed faces corresponded to the dense bands and were very similar in size in each parasite., Nov. 1991, 38, 6, S171, S172
  • Refereed, JOURNAL OF PROTOZOOLOGY, SOC PROTOZOOLOGISTS, ULTRASTRUCTURAL-LOCALIZATION OF EPITOPES RECOGNIZED BY MONOCLONAL-ANTIBODIES PRODUCED TO RAT PNEUMOCYSTIS-CARINII, H YOSHIKAWA; LE DEBAULT; DC GRAVES, The binding sites of five monoclonal antibodies (MAb) developed against rat Pneumocystis carinii were examined at the ultrastructural level by using a post-embedding labeling method. Although all five MAb reacted with the pellicle of P. carinii, they were divided into two groups by localization of binding sites. The MAb 168.2.1, 174.2.1, and 215.2.1 reacted mainly with the electron-dense outer layer, whereas MAb 227.1.1 and 228.1.1 labeled both the outer dense layer and the middle lucent layer. With in the first group of MAb, no significant differences were observed in the reactivity patterns seen with the different stages of P. carinii. In the second group, however, the intensity of labeling of the electron-dense layer was higher in the precyst, cyst, and ruptured cyst stages than in the trophozoite stage. These latter results indicate that there may be an increase in antigen accumulation during development from the trophozoite to the cyst stages, or that antigens may be modified the development., Nov. 1991, 38, 6, S18, S20
  • Refereed, The Journal of Protozoology, Wiley, Freeze-Fracture Study of the Bloodstream Form of Trypanosoma brucei gambiense, HISAO YOSHIKAWA; JUNKO FURUKI; YUZO TAKAHASHI; HIROYUKI MORIOKA; YUKIO YOSHIDA, Jan. 1990, 37, 1, 27, 32, Scientific journal
  • Refereed, APMIS, Wiley, Bromodeoxyuridine labeling studies on the proliferation of intestinal mucosal mast cells in normal and athymic rats, NAOKI Arizono; TSUNEZO Shiota; MINORU Yamada; YOSHITSUGU Matsumoto; HISAO Yoshikawa; SHINJI Matsuda; TATSUYA Tegoshi, Jan. 1990, 98, 1-6, 369, 376, Scientific journal
  • Refereed, Virchows Archiv A Pathological Anatomy and Histopathology, Springer Science and Business Media LLC, Disseminated infection ofPneumocystis carinii in a patient with the acquired immunodeficiency syndrome, Shinji Matsuda; Yohji Urata; Tsunezo Shiota; Minoru Yamada; Hisao Yoshikawa; Tatsuya Tegoshi; Mitsunori Okada; Hayato Nakamura; Toshio Kitaoka; Tsukasa Ashihara; Yukio Yoshida, 1989, 414, 6, 523, 527, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Variations in egg size ofTrichuris trichiura, H. Yoshikawa; M. Yamada; Y. Matsumoto; Y. Yoshida, 1989, 75, 8, 649, 654, Scientific journal
  • Refereed, The Journal of Protozoology, Wiley, The Present Status ofPneumocystis cariniiPneumonia in Japan with Special Reference to AIDS, YUKIO YOSHIDA; TSUXEZO SHIOTA; MINORU YAMADA; HISAO YOSHIKAWA; TATSUYA TEGOSHI; TSUYOSHI IKAI; KENJI OGINO, Jan. 1989, 36, 1, 78S, 80S, Scientific journal
  • Refereed, The Journal of Protozoology, Wiley, Detection of Surface Carbohydratesof Pneumocystis carinii, HISAO YOSHIKAWA; TATSUYA TEGOSHI; YUKIO YOSHIDA, Jan. 1989, 36, 1, 63S, 64S, Scientific journal
  • Refereed, The Journal of Protozoology, Wiley, Ultrastructure of Pneumocystis carinii: A Freeze-Fracture Study, HISAO YOSHIKAWA; YUKIO YOSHIDA, Jan. 1989, 36, 1, 51S, 52S, Scientific journal
  • Refereed, The Journal of Protozoology, Wiley, Freeze-Fracture Study of Blastocystis hominis, HISAO YOSHIKAWA; MINORU YAMADA; YUKIO YOSHIDA, Nov. 1988, 35, 4, 522, 528, Scientific journal
  • Refereed, The Journal of Protozoology, Wiley, Freeze-Fracture Study of the Trophozoite and the Cyst ofEntamoeba histolytica, HISAO YOSHIKAWA; MINORU YAMADA; YUKIO YOSHIDA, May 1988, 35, 2, 268, 273, Scientific journal
  • Refereed, International Journal for Parasitology, Elsevier BV, Distribution of digitonin-sterol complexes in Pneumocystis carinii revealed by freeze-fracture method, Hisao Yoshikawa; Yukio Yoshida, Feb. 1988, 18, 1, 39, 45, Scientific journal
  • Refereed, Parasitology Research, Springer Science and Business Media LLC, Ultrastructural detection of carbohydrates in the pellicle of Pneumocystis carinii, H. Yoshikawa; H. Morioka; Y. Yoshida, 1988, 74, 6, 537, 543, Scientific journal
  • Refereed, JOURNAL OF PROTOZOOLOGY, SOC PROTOZOOLOGISTS, FREEZE-FRACTURE LOCALIZATION OF FILIPIN-STEROL COMPLEXES IN PLASMA-MEMBRANES AND CYTO-MEMBRANES OF PNEUMOCYSTIS-CARINII, H YOSHIKAWA; H MORIOKA; Y YOSHIDA, May 1987, 34, 2, 131, 137, Scientific journal
  • Refereed, ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, GUSTAV FISCHER VERLAG, LOCALIZATION OF SILVER DEPOSITS ON PNEUMOCYSTIS-CARINII TREATED WITH GOMORI METHENAMINE SILVER-NITRATE STAIN, H YOSHIKAWA; Y YOSHIDA, May 1987, 264, 3-4, 363, 372, Scientific journal
  • Refereed, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, SPRINGER VERLAG, LIGHT MICROSCOPIC STUDY OF BLASTOCYSTIS SPP IN MONKEYS AND FOWLS, M YAMADA; H YOSHIKAWA; T TEGOSHI; Y MATSUMOTO; T YOSHIKAWA; T SHIOTA; Y YOSHIDA, 1987, 73, 6, 527, 531, Scientific journal
  • Refereed, The Japanese Journal of Parasitology, Effect of working time and temperature of sulfuric ether in the Toluidine Blue O stain for Pneumocystis carinii. (共著), YOSHIKAWA Hisao; M. Yamada, 1987, 36, 6, 371-374
  • Refereed, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, SPRINGER VERLAG, DETECTION OF SURFACE CARBOHYDRATES ON PNEUMOCYSTIS-CARINII BY FLUORESCEIN-CONJUGATED LECTINS, H YOSHIKAWA; T TEGOSHI; Y YOSHIDA, 1987, 74, 1, 43, 49, Scientific journal
  • Refereed, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, SPRINGER VERLAG, FREEZE-FRACTURE STUDIES ON PNEUMOCYSTIS-CARINII .2. FINE-STRUCTURE OF THE TROPHOZOITE, H YOSHIKAWA; H MORIOKA; Y YOSHIDA, 1987, 73, 2, 132, 139, Scientific journal
  • Refereed, Japan. J. Trop. Med. Hyg., Clinical trial of trimethoprim-sulfamethoxazole in the treatment of vivax, ovale and falciparum malaria. (共著), YOSHIKAWA Hisao; Y. Yoshida, 1987, 15, 2, 105-116
  • Refereed, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, SPRINGER VERLAG, FREEZE-FRACTURE STUDIES ON PNEUMOCYSTIS-CARINII .3. FINE-STRUCTURE OF THE PRECYST AND THE CYST, H YOSHIKAWA; Y YOSHIDA, 1987, 74, 1, 36, 42, Scientific journal
  • Refereed, ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH, SPRINGER VERLAG, FREEZE-FRACTURE STUDIES ON PNEUMOCYSTIS-CARINII .1. STRUCTURAL ALTERATION OF THE PELLICLE DURING THE DEVELOPMENT FROM TROPHOZOITE TO CYST, H YOSHIKAWA; Y YOSHIDA, 1986, 72, 4, 463, 477, Scientific journal
  • Refereed, 日本熱帯医学会雑誌, Trimethoprim-SulfamethoxazoleおよびPyrimethamine-SulfamonomethoxineによるPneumocystis carinii肺炎の発症予防に関する実験的研究 (共著), YOSHIKAWA Hisao, 1985, 13, 4, 287-294
  • Refereed, ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY, GUSTAV FISCHER VERLAG, PNEUMOCYSTIS-CARINII - ELECTRON-MICROSCOPIC INVESTIGATION ON THE INTERACTION OF TROPHOZOITE AND ALVEOLAR LINING CELL, Y YOSHIDA; Y MATSUMOTO; M YAMADA; K OKABAYASHI; H YOSHIKAWA; M NAKAZAWA, 1984, 256, 3, 390, 399, Scientific journal
  • Refereed, Southeast Asian Journal Trop. Med. Pub. Hlth., Report of an imported case of Dipetalonema perstans in Japan. (共著), YOSHIKAWA Hisao; Y. Yoshida, 1983, 14, 3, 341-344
  • Clinical Parasitology, Pathogenicity of Blastocystis spp., Masaharu Tokoro; Hisao Yoshikawa, Feb. 2021, 32, 1, 15, 18, Scientific journal

MISC

  • Refereed, 検査と技術, 腸管寄生原虫類の遺伝子検査法, YOSHIKAWA Hisao, Jun. 2012, 40, 6, 478-486
  • Not Refereed, 検査と技術, 腸管寄生原虫ブラストシスチスとその検査法, YOSHIKAWA Hisao, Jan. 2011, 39, 1, 11-17
  • Not Refereed, 臨床検査, 最近注目され始めた腸管寄生原虫Blastocystis hominisの検査法と鑑別診断法, YOSHIKAWA Hisao, Aug. 1997, 41, 8, 938-943
  • Not Refereed, Hitachi Scienetific Instrument News, フリーズレプリカ装置FR-7000を用いた応用例, YOSHIKAWA Hisao, Mar. 1997, 39, 2, 14-17
  • Not Refereed, 診断と治療, プラジカンテルによる肝吸虫症の治療 (共著), YOSHIKAWA Hisao, 1991, 79, 1, 155-158
  • Not Refereed, 医学のあゆみ, フリーズフラクチャー法による寄生原虫の研究, YOSHIKAWA Hisao, 1989, 148, 2, 84
  • Not Refereed, Memoris of the Konan University Science Series, キロショウジョウバエ温度感受性突然変異Shibirets1の翅原基の腫瘍化 (共著), YOSHIKAWA Hisao, 1988, 35, 1, 31-38

Books etc

  • Materials and Methods in Parasitology, 三恵社, YOSHIKAWA Hisao, 分担執筆, 2013, 1-4; 21-24, Not Refereed
  • Parasitology Research Monographs 4: Blastocystis:Pathogen or Passenger? An evaluation of 101 years of research, Springer, Heinz Mehlhorn; Kevin S.W. Tan; Hisao Yoshikawa, Editor & Author, Dec. 2012, 編集者&執筆者, Not Refereed
  • Molecular Detection of Human Parasitic Pathogens. Blastocystis, CRC Press, YOSHIKAWA Hisao, Author, Jul. 2012, 39-51, Not Refereed
  • スタンダード微生物学(第2版), 文光堂, YOSHIKAWA Hisao, 分担執筆, Feb. 2008, 350-362, Not Refereed
  • Parasitic Zoonosis in Asian-Pacific Regions 2006, Sankei-sha Co. Ltd., YOSHIKAWA Hisao, 分担執筆, Dec. 2006, 16, 20-31, Not Refereed
  • スタンダード微生物学, 文光堂, YOSHIKAWA Hisao, 分担執筆, Mar. 2005, 351-362, Not Refereed
  • 虫の知らせ, 三恵社, YOSHIKAWA Hisao, 分担執筆, Jul. 2002, Not Refereed
  • Isolation and characterization of Blastocystis spp. isolated from anuran amphibians(共著), 9th International Corgress of Parasitology. International Proceedings Division., H. Yoshikawa; K. Morimoto; M. Nagashima, pp. 1075-1079., 1998, 1075-1079頁, Not Refereed
  • Cyst form of Blastocystis pythoni in vitro culture. (共著), Electron Microscopy Vol. IV Biological Sciences, H. Yoshikawa; M. Nagashima; K. Morimoto, pp. 403-404, 1998, 4巻403-404頁, Not Refereed
  • 図説細胞生物学, 丸善, YOSHIKAWA Hisao, 分担執筆, 1998, 150-159, Not Refereed
  • 978-3-642-32737-7

Presentations

  • Oral presentation, 25 Sep. 2021, 25 Sep. 2021
  • Oral presentation, 25 Sep. 2021, 25 Sep. 2021
  • Oral presentation, 16 Apr. 2021, 17 Apr. 2021
  • Poster presentation, 30 May 2020, 31 May 2020
  • 横江彩子、吉川尚男, 第75回日本寄生虫学会西日本支部大会, Blastocystisのゲノム上のSSU rRNA遺伝子コピー間の 塩基配列の違いについて: ST6とST7に分類されるヒト由来株と鳥類由来株間の比較, 21 Sep. 2019, 21 Sep. 2019, 22 Sep. 2019
  • 辻本あかり、吉川尚男, 第75回日本寄生虫学会西日本支部大会, 複数のサブタイプを混合培養した際のBlastocystisの増殖について, 21 Sep. 2019, 21 Sep. 2019, 22 Sep. 2019
  • YOSHIKAWA Hisao, 第88回日本寄生虫学会大会, ブラストシスチス属の人獣共通感染性、インドネシア・スンバ島の10家族の住民とその家畜動物から分離された株について, Oral presentation, Mar. 2019, 長崎大学, False
  • Hisao Yoshikawa, The 2nd International Blastocystis Conference, Blastocystis and zoonotic potential: Molecular epidemiological approach to residents and their domestic animals among 10 families in a small Indonesian community with poor hygiene., Keynote oral presentation, 12 Sep. 2018, 11 Sep. 2018, 12 Sep. 2018
  • H. Yoshikawa; A. Iwamasa; M. Tokoro; T. Nagamoto; D. Syafruddin, 15th International Congress of Protistology, Molecular study of Blastocystis isolates from residents and their domestic animals among 10 families in a small Indonesian community with poor hygiene., Poster presentation, 01 Aug. 2017, rm:research_project_id
  • YOSHIKAWA Hisao, 第86回日本寄生虫学会大会, 奈良市内の環境水からのアカントアメーバの分離と遺伝子型解析, Oral presentation, May 2017, 北海道大学・学術交流会館, False
  • YOSHIKAWA Hisao, 第72回日本寄生虫学会西日本支部大会, インドネシア・スンバ島の住民と家畜動物間のブラストシスチス感染伝播について(2), Oral presentation, Oct. 2016, 岐阜市, False
  • YOSHIKAWA Hisao, 第72回日本寄生虫学会西日本支部大会, インドネシア・スンバ島で捕獲したゴキブリから分離されたブラストシスチス株について, Oral presentation, Oct. 2016, False
  • YOSHIKAWA Hisao, 第72回日本寄生虫学会西日本支部大会, インドネシアで分離されたヒト・ブラストシスチス分離株のゲノム多型について, Oral presentation, Oct. 2016, 岐阜市, False
  • YOSHIKAWA Hisao, 第72回日本寄生虫学会西日本支部大会, カエルから分離されたブラストシスチスの分子系統解析, Oral presentation, Oct. 2016, 岐阜市, False
  • YOSHIKAWA Hisao, 第72回日本寄生虫学会西日本支部大会, 環境水からのアカントアメーバの分離の試み, Oral presentation, Oct. 2016, 岐阜市, False
  • YOSHIKAWA Hisao, 第85回日本寄生虫学会大会, ブラストシスチス属の人獣共通感染性、インドネシア・スンバ島住民とその家畜から分離された株について, Oral presentation, Mar. 2016, 宮崎, False
  • YOSHIKAWA Hisao, 第71回日本寄生虫学会西日本支部大会, 爬虫類から分離したブラストシスチス株の分子系統解析, Oral presentation, Oct. 2015, 奈良, False
  • YOSHIKAWA Hisao, 第71回日本寄生虫学会西日本支部大会, インドネシア・スンバ島の住民と家畜動物間のブラストシスチス感染伝播について, Oral presentation, Oct. 2015, 奈良, False
  • Hisao Yoshikawa, Ayana Iwamasa, The 1 st International Blastocystis Symposium, Human Blastocystis Subtyping by a Combination of RFLP Analysis and Subtype-Specific Primers, Invited oral presentation, 29 May 2015, 28 May 2015, 29 May 2015
  • Hisao Yoshikawa, Masaharu Tokoro, Din Syafulddin, The 1 st International Blastocystis Symposium, Host Specificity of Blastocystis Subtypes: Comparison of Humans with Domestic Animals in a Small Community in Sumba Island, Indonesia, Invited oral presentation, 29 May 2015, 28 May 2015, 29 May 2015
  • Hisao Yoshikawa, The 1st International Blastocystis Symposium., Blastocystis phylogeny among a variety isolates from humans to insects., Keynote oral presentation, 28 May 2015, 28 May 2015, 29 May 2015
  • YOSHIKAWA Hisao, 第84回日本寄生虫学会大会, 遺伝的多型のヒトブラストシスチス株を簡便にサブタイプ分類するためのプライマー構築, Mar. 2015, 杏林大学医学部, False
  • YOSHIKAWA Hisao, 第70回日本寄生虫学会西日本支部大会, 爬虫類由来株のブラストシスチスの遺伝的多様性について, Oct. 2014, 兵庫医療大学薬学部微生物学分野, 神戸市・兵庫医療大学, False
  • YOSHIKAWA Hisao, 第70回日本寄生虫学会西日本支部大会, ブラストシスチス株の簡便なサブタイプ分類と系統解析のためのプライマー設計, Oct. 2014, 兵庫医療大学薬学部微生物分野, 神戸市・兵庫医療大学, False
  • Hisao Yoshikawa; Masaharu Tokoro; Din Syafruddin, 13th International Congress of Parasitology, Host-specificity of Blastocystis subtypes: Comparison of humans with domestic pigs and wild rodents in a small community in Sumba Island, Indonesia, 12 Aug. 2014
  • Masaharu Tokoro; Hisao Yoshikawa; Din Syafruddin, 13th International Congress of Parasitology, Genetic diversity of Giardia intestinalis detected from humans: Is there any geographical distribution pattern?, 11 Aug. 2014
  • YOSHIKAWA Hisao, 第83回日本寄生虫学会大会, ブラストシスチス属の遺伝的多様性と宿主特異性:特に日本とインドネシアのげっ歯類から分離された株について, Mar. 2014, 愛媛大学, False
  • YOSHIKAWA Hisao, 第69回日本寄生虫学会西日本支部大会, 爬虫類由来ブラストシスチス株の性状と遺伝的多様性, Oct. 2013, 高松市、香川, False
  • YOSHIKAWA Hisao, 第69回日本寄生虫学会西日本支部大会, 日本とインドネシアのげっ歯類由来のブラストシスチス株の遺伝的多様性, Oct. 2013, 高松市、香川, False
  • YOSHIKAWA Hisao, 第69回日本寄生虫学会西日本支部大会, 糞便検体からのDNA精製効率改善を目指した試み, Oct. 2013, 高松市、香川, False
  • YOSHIKAWA Hisao; Hisao Yoshikawa; Masaharu Tokoro; Din Syafruddin, 24th Molecular Parasitology Meeting, Spcificity of PCRprimers developed for Blastocystis subtype identification, Sep. 2013, True
  • YOSHIKAWA Hisao; Masaharu Tokoro; Hisao Yoshikawa; Din Syafruddin, 24th Molecular Parasitology Meeting, A comprehensive molecular analysis for various intestinal protozoan parasites., Sep. 2013, True
  • YOSHIKAWA Hisao; Hisao Yoshikawa, Internation Workshop on Enteric Protozoan Infections, A Unique Intestinal Protozoan Parasite, Blastocystis spp. --Epidemiology and Zoonotic Potential--, Feb. 2013, National Institute of Infectious Diseases, 東京, False
  • YOSHIKAWA Hisao; M. Tokoro; H. Yoshikawa; D. Syafruddin, The 12th Asia-Pacific Congree for Parasitic Zoonoses, A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia., Oct. 2012, Kobe, Japan, True
  • YOSHIKAWA Hisao; M. Katsumata; H. Yoshikawa; M. Tokoro; D. Syafruddin, The 12th Asia-Pacific Congree for Parasitic Zoonoses, Molecular phylogeny of Blastocystis isolates from rodents in the Sumba Island, Indonesia, Oct. 2012, Kobe, Japan, True
  • YOSHIKAWA Hisao; S. Arayama; S. Nakazawa; H. Yoshikawa; M. Tokoro, The 12th Asia-Pacific Congree for Parasitic Zoonoses, Improvement of molecular detection methods for intestinal protozoan parasites., Oct. 2012, Kobe, Japan, True
  • YOSHIKAWA Hisao, 第68回日本寄生虫学会西日本支部大会, ブラストシスチスのサブタイプ同定に用いられているPCRプライマーの特異性について, Oct. 2012, 奈良, False
  • YOSHIKAWA Hisao, 第68回日本寄生虫学会西日本支部大会, 動物園で飼育されている爬虫類からのブラストシスチス株分離の試み, Oct. 2012, 奈良, False
  • YOSHIKAWA Hisao, 第68回日本寄生虫学会西日本支部大会, 爬虫類由来ブラストシスチス株の性状と遺伝的多様性について, Oct. 2012, 奈良, False
  • YOSHIKAWA Hisao; M. Tokoro; H. Yoshikawa; D. Syafruddin, 15th International Congress of Infectious Diseases, A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia., Jun. 2012, Bangkok, Thailand, True
  • YOSHIKAWA Hisao, 第23回日本臨床寄生虫学会, Entamoeba dispar感染にヒトブラストシスチス及び小形アメーバが検出されたブラジル人症例と回盲部に潰瘍を認めた無症候性E. histolytica感染の日本人症例, Jun. 2012, 東京, False
  • YOSHIKAWA Hisao, 第81回日本寄生虫学会大会, Comprehensive molecular detection methods revealed a true image of protozoan endemicity in Southwest Sumba district, Indonesia, Mar. 2012, 日本寄生虫学会, 兵庫医科大学、西宮, False
  • YOSHIKAWA Hisao, 第81回日本寄生虫学会大会, ブラストシスチスの感染伝播に関する研究:2009年に実施したインドネシア・スンバ島での調査, Mar. 2012, 日本寄生虫学会, 兵庫医科大学、西宮, False
  • YOSHIKAWA Hisao; Hisao Yoshikawa, University of Leicester, College of Medicine, Biological Science & Psychology, Diversity of the genus Blastocystis, Nov. 2011, Leicester, England, True
  • YOSHIKAWA Hisao, 第67回日本寄生虫学会西日本支部大会, 蛍光抗体試薬のブラストシスチス検出感度について, Oct. 2011, 日本寄生虫学会, 金沢大学, False
  • YOSHIKAWA Hisao, 第67回日本寄生虫学会西日本支部大会, サブタイプ1から9までのブラストシスチス株のミトコンドリア遺伝子ND3による系統解析, Oct. 2011, 日本寄生虫学会, 金沢大学, False
  • YOSHIKAWA Hisao, 第67回日本寄生虫学会西日本支部大会, ブラストシスチス属の分子分類の現状と問題点, Oct. 2011, 所 正治, 金沢大学, False
  • YOSHIKAWA Hisao; Hisao Yoshikawa; Mayumi Murakami; Zhiliang Wu; Tetsuo Yanagi, 17th Japanese-German Cooperative Symposium on Protozoan Diseases and Workshop of Gastrointestinal Protozoan Diseases, Molecular phylogenetic studies of Blastocystis isolates from manrsupial mammals., Sep. 2011, The Japanese-German Association on Protozoan Diseases, Nara Women's University, Nara, True
  • YOSHIKAWA Hisao; Norishige Yamamoto; Marina Kon; Mahaharu Tokoro; Motohiro Iseki; Isao Kimata; Hisao Yoshikawa, 17th Japanese-German Cooperative Symposium on Protozoan Diseases and Workshop of Gastrointestinal Protozoan Diseases, Massive waterborne outbreaks of cryptosporidiosis in Japan, Sep. 2011, The Japanese-German Association on Protozoan Diseases, Nara Women's University, Nara, True
  • YOSHIKAWA Hisao, 第80回日本寄生虫学会大会, ミトコンドリアNADHデヒドロゲナーゼND3遺伝子によるブラストシスチス株の分子系統解析, Jul. 2011, 日本寄生虫学会, 東京慈恵会医科大学、東京, False
  • YOSHIKAWA Hisao; H. Yoshikawa; F Dogruman-AI; S. Turk; S. Kustimur; N. Balaban; N. Sultan, XXXIV Turkish Microbiology Congress, Comparison of commercial DNA isolation kits for molecular diagosis of Blastocystis protozoon with culture method., Sep. 2010, True
  • YOSHIKAWA Hisao, 第79回日本寄生虫学会大会, ブラストシスチスの診断・検出法の評価:市販キットによる糞便からの直接DNA抽出-PCR法と培養法の比較, May 2010, 日本寄生虫学会, 北海道、旭川市, False
  • YOSHIKAWA Hisao; Hisao Yoshikawa, Gazi University in Turkey, Diversity and phylogeny in the genus Blastocystis, Sep. 2009, Department of Medical Microbiology, Gazi University in Turkey, Ankara, True
  • YOSHIKAWA Hisao, 第78回日本寄生虫学会大会, トルコの3病院下痢症患者と無症状患者におけるブラストシスチス感染状況と分離株のサブタイプ分類, Mar. 2009, 日本寄生虫学会, 順天堂大学、東京, False
  • YOSHIKAWA Hisao, 第64回日本寄生虫学会西日本支部大会, ブラストシスチスの新規遺伝子型サブタイプ8と9を検出するプライマーの作製, Nov. 2008, False
  • YOSHIKAWA Hisao, 第55回日本臨床検査医学会学術集会, 当院で経験したBlastocystis hominisの3例, Nov. 2008, 名古屋国際会議場, False
  • YOSHIKAWA Hisao; Hisao Yoshikawa; Aya Iguchi; Minoru Yamada; Isao Kimata; Naoki Arizono, 16th Japanese-German Cooperative Symposium on Protozoan Diseases, Inflammatory response in rats infected with Blastocystis hominis., Sep. 2008, Gottingen, Germany, True
  • YOSHIKAWA Hisao; Hisao Yoshikawa; Zhiliang Wu; Kishor Pandy; Tetsuo Yanagi; Hiroji Kanbara, The Tenth Asian-Pacific Congress for Parasitic Zoonoses, Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Aug. 2008, Taiwan, True
  • YOSHIKAWA Hisao, 第77回日本寄生虫学会大会, ブラストシスチス培養細胞と感染動物から得たシスト型の単一細胞からのSSU rRNA遺伝子配列の比較, Apr. 2008, False
  • YOSHIKAWA Hisao, 第63回日本寄生虫学会西日本支部大会, 有袋類のカンガルーとワラビーから分離されたブラストシスチス株の分子系統学的位置関係, Oct. 2007, False
  • YOSHIKAWA Hisao, 第63回日本寄生虫学会西日本支部大会, ブラストシスチス栄養型とシスト型の単一細胞からのSSU rRNA遺伝子配列の比較, Oct. 2007, 日本寄生虫学会, False
  • YOSHIKAWA Hisao, 第63回日本寄生虫学会西日本支部大会, ブラストシスチスの感染動物モデルの開発(10):感染ラットにおける抗体産生について, Oct. 2007, False
  • YOSHIKAWA Hisao, 第76回日本寄生虫学会大会, ブラストシスチスの感染動物モデルの開発(9):ラット由来RN94-9株の病原性について, Mar. 2007, False
  • YOSHIKAWA Hisao; Hisao Yoshikawa, Nanjing Medical University, Recent advances on Blastocystis research, Nov. 2006, 南京医科大学 病原性微生物学教室, 南京医科大学, True
  • YOSHIKAWA Hisao, 第62回日本寄生虫学会西日本支部大会, ブラストシスチスの分子系統的解析:哺乳類から昆虫類まで, Nov. 2006, False
  • YOSHIKAWA Hisao, 第62回日本寄生虫学会西日本支部大会, 分類困難な寄生原虫の分子系統によるアプローチ, Nov. 2006, 愛知医科大学寄生虫学講座, 愛知県, False
  • YOSHIKAWA Hisao; Hisao Yoshikawa; Aya Iguchi; Akiko Ebisu; Satiko Nagata; Yumie Saitou; Satoru Iwatani; Isao Kimata, 15th Japanese-German Cooperative Symposium on Protozoan Diseases, Infectivity of human Blastocystis hominis isolates in chickens and rats., Sep. 2006, True
  • YOSHIKAWA Hisao; H. Yoshikawa; Z. Wu; J. Howe; N. Geok-choo; KSW Tan, XIth International Congress of Parasitology, Phylogenetic study of Blastocystis isolates from mammals, birds, reptilians, amphibians and insects., Aug. 2006, England, True
  • YOSHIKAWA Hisao; Y. Saitou; A. Iguchi; S. Iwatani; I. Kimata; H. Yoshikawa, The 9th Asian-Pasific Congress for Parasitic Zoonosis, Zoonotic potential and cyst passage of human Blastocystis hominis., Aug. 2006, Gifu, Japan, True
  • YOSHIKAWA Hisao; A. Iguchi; H. Yoshikawa; M. Yamada; I. Kimata; N. Arizono, The 9th Asian-Pasific Congres for Parasitic Zoonosis, Infectivity and pathogenicity of Blastocystis., Aug. 2006, Gifu, Japan, True
  • YOSHIKAWA Hisao; Hisao Yoshikawa, The 9th Asian-Pasific Congress for Parasitic Zoonosis, Diversity and phylogeny in the genus Blastocystis, Aug. 2006, Prof. Dr. Yuzo Takahashi, Gifu University, True
  • YOSHIKAWA Hisao, 第75回日本寄生虫学会大会, ネパール、カトマンドゥの小児から分離したブラストシスチス株の遺伝子型と感染由来について, May 2006
  • YOSHIKAWA Hisao; H. Yoshikawa, National University of Singapore, Zoonotic potential of human Blastocystis, Oct. 2005, Dr. KSW Tan, Singapore, True
  • YOSHIKAWA Hisao; H. Yoshikawa, National Institute of Parasitic Diseases & Chinese Center for Disease Control, Shanghai, China, Biology and molecular aspects on Blastocystis., Sep. 2005, Dr. Zhou, China, True
  • YOSHIKAWA Hisao; H. Yoshikawa, Khon Kaen University, Thailand, Genomic polymorphism among Blastocystis isolates from human and animals., Aug. 2005, Deapartment of Parasitology, Thailand, True
  • YOSHIKAWA Hisao, 平成17年度奈良女子大学大学院人間文化研究科 公開講座, 寄生性原生生物:大家と住人の関係, Aug. 2005, 奈良女子大学大学院人間文化研究科, 奈良市, False
  • 横江彩子; 吉川尚男, 第76回日本寄生虫学会西日本支部大会, ブラストシスチスのゲノム上のSSU rRNA遺伝子コピー間の塩基配列の違いとND3遺伝子の多様性との比較, 25 Sep. 2021, 25 Sep. 2021, 25 Sep. 2021
  • 船木美奈; 吉川尚男, 第76回日本寄生虫学会西日本支部大会, ヒト由来ブラストシスチスの分子系統的に異なる9種類のサブタイプを検出する2種類の方法の比較, 25 Sep. 2021, 25 Sep. 2021, 25 Sep. 2021
  • Hisao Yoshikawa; Andini Nurfatimah Karim, 第90回日本寄生虫学会/第32回日本臨床寄生虫学会合同大会, Genetic polymorphism of the genus Blastocystis spp.: diversity among the isolates from wild frogs in Indonesia and Japan, 16 Apr. 2021, 16 Apr. 2021, 17 Apr. 2021
  • 吉川尚男、竹谷美希、所正治、ディン・シャフルディン, 第89回日本寄生虫学会大会, インドネシア・スンバ島で捕獲したゴキブリから分離されたブラストシスチスの分子系統解析, 30 May 2020, 30 May 2020, 31 May 2020
  • Andini Nurfatimah Karim, 第75回日本寄生虫学会西日本支部大会, Isolation and characterization of Blastocystis isolates from Indonesian frogs, 21 Sep. 2019, 21 Sep. 2019, 22 Sep. 2019

Works

  • Molecular subtyping of human Blastocystis isolates in Thailand, Sep. 2010
  • Molecular subtyping of human Blastocystis isolates in Thailand, Sep. 2010
  • 腸管内感染原虫症の疫学研究, Apr. 2005, Mar. 2010
  • A survey of Blastocystis infection in humans and animals in Sumba Island, Indonesia, Aug. 2009
  • A survey of Blastocystis infection in humans and animals in Sumba Island, Indonesia, Aug. 2009
  • A survey of human Blastocystis in China, Aug. 2005, Mar. 2008
  • A survey of human Blastocystis in China, Aug. 2005, Mar. 2008
  • ブラストシスチスの病原性に関する研究, 2006, 2008
  • A survey of Blastocystis hominis infection and genotype classification of B. hominis isolates in Thailand, 2005, 2007
  • A survey of Blastocystis hominis infection and genotype classification of B. hominis isolates in Thailand, 2005, 2007
  • Molecular phylogenetic study on human and animal Blastocystis in Turkey, Aug. 2006
  • Molecular phylogenetic study on human and animal Blastocystis in Turkey, Aug. 2006
  • 生物の初期進化の解明:研究の現状とデータ解析の問題点, Apr. 2002, Mar. 2003
  • クリプトスポリジウムの分子生物学的研究, Apr. 2000, Mar. 2003
  • Molecular biological studies on Cryptosporidium, Apr. 2000, Mar. 2003
  • 金属錯体界面活性剤から成るマイクロエマルジョン系による金属ナノ粒子の制御, Apr. 2000, Mar. 2002
  • A control of metal nanoparticles by microemulsion systems composed of metal complex surfactants, Apr. 2000, Mar. 2002
  • ミトコンドリアを持たない真核生物の系統的位置づけと真核生物の初期進化, Apr. 1996, Mar. 1999
  • 腸管内感染寄生原虫の分離と保存に関する研究, 1999
  • 腸管内寄生原虫ブラストシスチスの分子生物学的研究, 1996
  • Biological study on Blastocystis in humans and animals, Sep. 1994
  • Biological study on Blastocystis in humans and animals, Sep. 1994

Awards

  • The third prize for poster presentation, Turkish Microbiology Committee, Hisao Yoshikawa, Nov. 2010
  • 風戸研究奨励賞, 財団法人風戸研究奨励会, 1992

Research Projects

  • Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Oct. 2019, Mar. 2023, 19KK0200, Study on the dynamics of initial infections and colonizations with intestinal protozoan parasites, 所 正治; 吉川 尚男; 松村 隆弘, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Kanazawa University, 18330000, 14100000, 4230000
  • Grant-in-Aid for Scientific Research (C), Apr. 2018, Mar. 2023, 18K07085, Principal investigator, Revalidation of zoonotic potential of Blastocystis based on the DNA samples obtained from endemic area of fecal-oral infection., 吉川 尚男, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Nara Women's University, 3640000, 2800000, 840000, 腸管寄生原虫ブラストシスチスは、ヒトから検出される最も頻度の高い寄生虫であり、人獣共通感染性と想定されてきた。しかし、我々の糞口感染地域に住む児童らの予備的調査では人獣共通感染性が見られなかった(Yoshikawa et al. 2016, Parasitol. Int. 65:780-784)。 本研究では、インドネシアの糞口感染地域の住民と、その住民が保有している家畜動物、ならびに住民の家屋内で捕獲した野ネズミ等から得た糞便材料から直接抽出したDNA材料を使い、ブラストシスチスの人獣共通感染性について再検証した。 本研究材料は、糞口感染性の寄生虫がまん延している地域に住む住民とその家畜動物、ならびに家屋内の野ネズミ等の新鮮な糞便材料から直接抽出したDNAである。そのため、ブラストシスチスが人獣共通感染性であるならば、住民・家畜動物・家屋内の野ネズミ等の糞便汚染により住民と動物から遺伝的に同じブラストシスチスが検出されるはずである。今回、家屋内で捕獲された野ネズミ由来のブラストシスチスを調べたところ、サブタイプ4のみが検出された。一方、住民の児童からはサブタイプ1,2,3が検出されており、住民が感染していたブラストシスチスと同じサブタイプは野ネズミからは検出されなかった。さらに、家畜動物のブタ、ニワトリ、ヤギ、水牛などを調べた結果、ブタからサブタイプ5、ニワトリからはサブタイプ6と7、ヤギからはサブタイプ1と5が検出され、水牛からは何も検出されなかった。一方、住民からは予備調査と同様に、サブタイプ1,2,3が検出された。住民が感染していたサブタイプと同じサブタイプ1が、ヤギからも検出されたため、これが同じブラストシスチス由来であるかどうかについて、現在遺伝子のシークエンス解析を行って詳細に調べている。
  • Grant-in-Aid for Scientific Research (B), Apr. 2014, Mar. 2017, 26305008, Coinvestigator, Comprehensive molecular epidemiological study for parasites distributing in Indonesia, Tokoro Masaharu, Kenji Kimura, Hisao Yoshikawa, Makoto Ito, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Kanazawa University, 16380000, 12600000, 3780000, Seasonal molecular epidemiology of Entamoeba spp. and Giardia intestinalis was evaluated using children’s and related animal fecal samples at a rural village in Indonesia. The higher positive rate of E. coli in rainy season (23.9%, vs. 33.7%, P=0.02 in dry and rainy seasons respectively) was observed, and the main factor that brings about this was thought to be the higher E. coli prevalence (11.5% vs. 20.6%, P=0.02, same as the above) in female. While, the genotyping evaluation of G. intestinalis confirmed the lower prevalence of both assemblage A and B among female population in rainy season (A: 5.9% vs. 0.8%, P=0.026; B: 16.9%, vs. 7.8%, P=0.035, same as the above), though there was no seasonal difference of those prevalences in male. The comprehensive molecular screening approaches for those various species and genotypes could address environmental and host-related factors effectively., rm:presentations
  • 2009, Mar. 2022, Principal investigator, Molecular phylogenetic study of human and animal Blastocystis in Sumba Island, Indonesia, 吉川尚男; 所正治; Din Syafruddin, 国際貢献国際共同研究, 0, 0, 0, Competitive research funding
  • 2003, 2022, Principal investigator, 腸管内寄生原虫ブラストシスチスの遺伝的多様性と人畜共通性との関連性の解明, 吉川尚男、所正治、Din Syafruddin, 国際貢献国際共同研究, 0, 0, 0, Competitive research funding
  • Grant-in-Aid for Scientific Research (B), Apr. 2011, Mar. 2015, 23406006, Coinvestigator, Study on genetic diversity of highly endemic pathogenic intestinal porotozans in Indonesia, MASAHARU Tokoro; YOSHIKAWA Hisao, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Kanazawa University, 15860000, 12200000, 3660000, The molecular taxonomic method was applied in investigations of various protozoan infections in Indonesia, and the endemicity data was epidemiologically assessed from the view of mutual relations with the characteristics, such as age, gender, of target population. The results showed following findings: (1) All the investigated protozoan groups, Amoebazoa, Fornicata, Parabasalia, and Stramenopiles, were highly endemic in the target site. (2) Age-related and gender-related differences of protozoan prevalences were indicated in targeted student population. (3) Giardial prevalence reduction on aging was attributed to assemblage A prevalence, which was reduced in higher age layers. (4) The endemicity of Pentatrichomonas hominis was maintained by zoonotic transmissions of a specific strain in the area. Molecular epidemiological methods targeting various protozoan species could improve our understanding of the endemicity of those infections., rm:published_papers;rm:published_papers
  • 2010, 2013, Coinvestigator, Molecular phylogenetic research on human Blastocystis isolates in Thailand., Somachai Pinlaor; Hisao Yoshikawa, 国際貢献国際共同研究, 0, 0, 0, Competitive research funding
  • 2008, 2011, Coinvestigator, Subtype classification of human Blastocystis isolates in Turkey., Funda Dogruman, 吉川尚男, 国際貢献国際共同研究, 0, 0, 0, Competitive research funding
  • Grant-in-Aid for Scientific Research (B), Apr. 2005, Mar. 2007, 17406007, Coinvestigator not use grants, Epidemiological survey and classification of genotypes of intestinal parasitic protozoa Blastocystis, YOSHIKAWA Hisao; WU Zhiliang, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Nara Women's University, 4550000, 4400000, 150000, Although, pathogenic potential of an intestinal parasite, Blastocystis hominis, had been proposed on the basis of clinical symptom, the true nature of the parasite infection in humans is still controversial. Since B. hominis is known to be genetically polymorphic, it is generally assumed that the certain genotype of the parasite may contribute to its pathogenic potential. Based on these backgrounds, this project was performed on the survey of human Blastocystis infection and determined genotypes among Blastocystis populations. Since the prevalence of human Blastocystis infection in Japan was lower than other developing Asian countries, this project was performed in Thailand, China and Singapore. Finally, a total 47 isolates were obtained from 2 different epidemiological setting in Thailand. In China, 270 isolates were obtained from 4 different epidemiological setting. In Singapore, only 9 isolates were obtained from a major hospital in Singapore. When 270 isolates in China was screened by PCR with our developed sequenced tagged-site primers, all of the isolates except for an isolate were classified into the known genotypes. Since an isolate was negative with any STS primers, entire sequence of the small subunit rRNA gene was sequence and phylogenetically analyzed with the known genotypes. Interestingly, an human isolates was positioned in an additional new clade with other 2 isolates reported in GenBank.
  • Grant-in-Aid for Scientific Research (C), Apr. 2004, Mar. 2006, 16590338, Principal investigator, Studies on genotype polymorphism, zoonotic potential and transmission of Blastocystis, YOSHIKAWA Hisao, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Nara Women's University, 3600000, 3600000, Ever since the first description of Blastocystis hominis found in human intestinal tract in 1912, this parasite has been widely isolated from a variety animals including non-human primates, mammals, birds, reptiles, amphibians and insects. Blastocystis isolates from mammals and birds are known to be classified into 7 kinds of different clades inferred from the small subunit rRNA genes. Since most of the clades shared between humans and animals, most human Blastocystis isolates have been assumed to be zoonotic. Most attempts undertaken to date to assess the zoonotic potential of human Blastocystis isolates in host animals have not been completely successful. This study demonstrated that rats are susceptible animals to both RN94-9 and NIH:1295-1 isolates recovered from a laboratory rat and a guinea pig, respectively. Moreover, chicks were susceptible to all of 6 Blastocystis isolates from chickens, quails and geese. Therefore, rats and chicks are useful animal model for Blastocystis infection. In addition, transmission of this parasite caused by ingestion of fecal cysts was also demonstrated in this study. Infectivity associated with several unique human Blastocystis genotypes was also investigated by infecting chicks and rats with two isolates of each genotype experimentally. Our results confirmed that several genotypes of human isolates could infect chicks and/or rats, indicating that chicks and rats are suitable experimental animals models for studying the zoonotic potential of human Blastocystis isolates.
  • 基盤研究(C), Apr. 2001, Mar. 2003, Principal investigator, ブラストシスチスのゲノム遺伝子の多様性を利用した人畜共通株の同定, 吉川尚男, 日本学術振興会 科学研究費助成事業 基盤研究(C), 0, 0, 0, Competitive research funding
  • 基盤研究(C), Apr. 1997, Mar. 1999, Coinvestigator, 腸管寄生原虫であるブラストシスチスの分類・系統に関する分子生物学的研究, 吉川尚男, 日本学術振興会 科学研究費助成事業 基盤研究(C), 科学研究費補助金, 奈良女子大学, 0, 0, 0, Competitive research funding
  • 基盤研究(C), Apr. 1994, 1995, ブラストシスチス・ホミニスの分類に関する電子顕微鏡的研究, 吉川尚男, 日本学術振興会 科学研究費助成事業 奨励研究, 0, 0, 0, Competitive research funding
  • 奨励研究, Apr. 1989, Mar. 1990, Principal investigator, ニューモシスチス・カリニ感染における肺上皮細胞表面の接着因子としての糖の存在, 吉川尚男, 日本学術振興会 科学研究費助成事業 奨励研究
  • 奨励研究, Apr. 1988, Mar. 1989, Principal investigator, レクチンによるニューモシスチス・カリニ表面に存在する糖の同定, 吉川尚男, 日本学術振興会 科学研究費助成事業 奨励研究, 京都府立医科大学

Ⅲ.社会連携活動実績

1.公的団体の委員等(審議会、国家試験委員、他大学評価委員,科研費審査委員等)

  • Parasitology Research, Reviewer, Jan. 2015, 9999, Society
  • 日独原虫病協会, 評議委員、監事, Apr. 2004, 9999, Society
  • 日本寄生虫学会, 評議委員, 1999, 9999, Society
  • 日本顕微鏡学会, 関西支部評議員, Apr. 1997, Mar. 2019, Society
  • Apr. 2001, Mar. 2007, Society
  • 1993, 1998, Society


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