SUGA Naoko

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Profile and Settings

• Name (Japanese)

  Suga

• Name (Kana)

  Naoko

Research Areas

• Life sciences, Nutrition and health science
• Life sciences, Food sciences

Research Experience

• Apr. 2023, 9999, Nara Women's University, Faculty Division of Human Life and Environmental Sciences, 助教
• Apr. 2021, Mar. 2023, Konan Women's University, Faculty of Human Sciences, 講師
• Apr. 2018, Mar. 2019, University of Hyogo, 環境人間学部食環境栄養課程, 特任助手

Education

• 2017, 2021, 兵庫県立大学大学院, 環境人間学研究科 博士後期課程
• 2015, 2017, University of Hyogo, 環境人間学研究科 博士前期課程
• 2011, 2015, University of Hyogo, 環境人間学部食環境栄養課程
• 2003, 2008, Nara Women's University, Faculty of Human Life and Environment

Teaching Experience

• Apr. 2021, Mar. 2024
• Apr. 2021, Mar. 2023
• Apr. 2021, Mar. 2023
• Apr. 2021, Mar. 2023
• Apr. 2021, Mar. 2023
• Apr. 2020, Mar. 2021
• Health and Nutrition, Shiga University, Oct. 2024, 9999
• Nutritional epidemiology, Kyoto Women's University, Sep. 2024, 9999
• Food Science, Shiga University, Apr. 2024, 9999
• Pathophysiology and biochemistry experiments, Nara Women's University, Apr. 2023, 9999
• Basic nutrition experiment, Nara Women's University, Apr. 2023, 9999
• Graduation Research I and II, Konan Women's University, Apr. 2021, Mar. 2023

Ⅱ．研究活動実績

Published Papers

• Refereed, 調理加工への応用を目指したマヌカハニー特有成分の熱安定性に関する検討, 菅 尚子; 焼本千里; 岡野やや子; 坂本 薫; 加藤陽二, Jul. 2021, 54, 4, 186, 192
• Journal of clinical biochemistry and nutrition, Luteolin suppresses 5-hydroxytryptamine elevation in stimulated RBL-2H3 cells and experimental colitis mice., Naoko Suga; Akira Murakami; Hideyuki Arimitsu; Toshiyuki Nakamura; Yoshimasa Nakamura; Yoji Kato, Increased 5-hydroxytryptamine may be associated with the development and progression of inflammatory bowel disease. In this study, we examined the suppressive effect of flavonoids on the increased intra- and extracellular 5-hydroxytryptamine levels in rat mast RBL-2H3 cells, known to produce 5-hydroxytryptamine by the phorbol 12-myristate 13-acetate stimulation. Among the flavonoids examined, luteolin and quercetin significantly reduced the cellular 5-hydroxytryptamine concentration. Gene and protein expression analyses revealed that luteolin significantly suppressed cellular tryptophan hydroxylase 1 expression induced by phorbol 12-myristate 13-acetate stimulation. Mitogen-activated protein kinase/extracellular signal-regulated kinase signaling was also suppressed by luteolin, suggesting that this pathway is one of targets of 5-hydroxytryptamine modulation by luteolin. An in vivo experimental colitis model was prepared by administering 2.5% dextran sodium sulfate in drinking water to C57BL/6 mice for seven days. The ingestion of 0.1% dietary luteolin suppressed the increasing 5-hydroxytryptamine in the colorectal mucosa. In conclusion, luteolin possesses a suppressive effect on extensive 5-hydroxytryptamine formation in both experimental RBL-2H3 cells and colitis models., Jul. 2021, 69, 1, 20, 27, Scientific journal, False, 10.3164/jcbn.20-192

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• Journal of clinical biochemistry and nutrition, Elevation of the serotonin-derived quinone, tryptamine-4,5-dione, in the intestine of ICR mice with dextran sulfate-induced colitis., Naoko Suga; Akira Murakami; Hideyuki Arimitsu; Kazuya Shiogama; Sarasa Tanaka; Mikiko Ito; Yoji Kato, Inflammatory bowel diseases, including Crohn's disease and ulcerative colitis, are chronic inflammatory disorders associated with oxidative stress. The intestines produce 5-hydroxytryptamine that may negatively affect disease state under inflammatory conditions when overproduced. 5-Hydroxytryptamine is a substrate for myeloperoxidase and is converted into reactive tryptamine-4,5-dione. Here, an experimental colitis model was established through oral administration of 5% dextran sulfate sodium to ICR mice for 7 days. Furthermore, the formation of tryptamine-4,5-dione in the colorectal mucosa/submucosa and colorectal tissue was analyzed by chemical and immunochemical methodologies. First, free tryptamine-4,5-dione in the homogenate was chemically trapped by o-phenylenediamine and analyzed as the stable phenazine derivative. Tryptamine-4,5-dione localization as adducted proteins in the colorectal tissue was immunohistochemically confirmed, and as demonstrated by both methods, this resulted in the significant increase of tryptamine-4,5-dione in dextran sulfate sodium-challenged mice compared with control mice. Immunohistochemical staining confirmed tryptamine-4,5-dione-positive staining at the myeloperoxidase accumulation site in dextran sulfate sodium-challenged mice colorectal tissue. The tryptamine-4,5-dione locus in the mice was partly matched with that of a specific marker for myeloperoxidase, halogenated tyrosine. Overall, the results possibly indicate that tryptamine-4,5-dione is generated by neutrophil myeloperoxidase in inflammatory tissue and may contribute to the development of inflammatory bowel disease., Jul. 2021, 69, 1, 61, 67, Scientific journal, False, 10.3164/jcbn.20-161

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• Food chemistry, Methylglyoxal binds to amines in honey matrix and 2'-methoxyacetophenone is released in gaseous form into the headspace on the heating of manuka honey., Yoji Kato; Yui Kishi; Yayako Okano; Masaki Kawai; Michiyo Shimizu; Naoko Suga; Chisato Yakemoto; Mai Kato; Akika Nagata; Noriyuki Miyoshi, Reports on the thermal stability of manuka honey in terms of food processing have been few. This study investigated changes in nine characteristic chemicals of manuka honey during heating. Among these, methylglyoxal (MGO) and 2'-methoxyacetophenone (MAP) were significantly decreased by heating at 90 °C. To elucidate the mechanism for this decrease, artificial honey was prepared from sugars and water with MAP or MGO and then heated. The decrease of MGO was enhanced with l-proline, lysine, or arginine derivatives, accompanied by formation of 2-acetyl-1-pyrroline, MGO-derived lysine dimer, or argpyrimidine, respectively, suggesting that an amino-carbonyl reaction is one pathway for the loss of MGO. The decrease of MAP in the artificial honey depended on the volume of headspace in a vessel. MAP from heated manuka honey was also detected in the gas phase, indicating that MAP was vaporized. Heating could thus reduce the beneficial and/or signature molecules in honey., 01 Feb. 2021, 337, 127789, 127789, Scientific journal, True, 10.1016/j.foodchem.2020.127789

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• Journal of clinical biochemistry and nutrition, Covalent adduction of endogenous and food-derived quinones to a protein: its biological significance., Yoji Kato; Naoko Suga, There are many chemically reactive compounds, including quinone, in living systems and also food. Even after the ingestion of food polyphenols, quinones derived from catechol moieties could form endogenously in the body. Dopaquinone, dopamine quinone, estrogen-derived quinones, tryptamine-4,5-dione, and ubiquinone are examples of an endogenous quinone. These indicate that quinone is ubiquitously formed or present in living systems and food. Quinones can induce a variety of hazardous effects and also could have beneficial physiological effects. This review focuses on the chemical reactivity of quinone toward a biomolecule and its biological action., May 2018, 62, 3, 213, 220, Scientific journal, False, 10.3164/jcbn.18-26

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• Free radical research, Cytotoxic and cytoprotective effects of tryptamine-4,5-dione on neuronal cells: a double-edged sword., Naoko Suga; Akira Murakami; Yoshimasa Nakamura; Akari Ishisaka; Noritoshi Kitamoto; Mikiko Ito; Yoji Kato, Serotonin (5-hydroxytryptamine) is a putative substrate for myeloperoxidase, which may convert it into the reactive quinone tryptamine-4,5-dione (TD). In this study, we found that the viability of human SH-SY5Y neuroblastoma cells treated with 25 μM TD was increased to approximately 117%. On the other hand, the cell viability was significantly decreased by exposure to TD (150-200 μM), with an increase in intracellular reactive oxygen species (ROS). Interestingly, pre-treatment of SH-SY5Y cells with 100 μM TD prevented cell death and suppressed intracellular ROS generation evoked by the addition of hydrogen peroxide (H2O2). Expression of the phase-II antioxidant enzyme NAD(P)H: quinone oxidoreductase 1 and haem oxygenase 1 were upregulated by TD at a concentration of 50-100 μM. Nuclear factor erythroid 2-related factor 2 (Nrf2), the regulator of these enzyme, was translocated from the cytosol to the nucleus by 100 μM TD. In summary, moderate concentrations of TD may increase the self-defence capacity of neuronal cells against oxidative stress., May 2017, 51, 5, 545, 553, Scientific journal, True, 10.1080/10715762.2017.1331038

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• Free radical biology & medicine, A novel quinone derived from 5-hydroxyindoleacetic acid reacts with protein: Possible participation of oxidation of serotonin and its metabolite in the development of atherosclerosis., Yoji Kato; Kota Oki; Naoko Suga; Shigeki Ono; Akari Ishisaka; Yoko Miura; Satoshi Kanazawa; Michitaka Naito; Noritoshi Kitamoto; Anthony J Kettle, The modification of 5-hydroxyindoleacetic acid (5HIAA) by myeloperoxidase with a xanthine oxidase system was investigated by chromatographic analyses. Two major products were identified as a dimer and quinone (indoleacetate dione) of 5HIAA. The formation of a quinone moiety was also confirmed by chemical trapping with o-phenylenediamine. In the presence of N-acetyl-cysteine (NAC), a quinone-NAC adduct was formed. When glyceraldehyde 3-phosphate dehydrogenase was exposed to the myeloperoxidase system with 5HIAA, quinone adducts were formed on the protein molecule. A monoclonal antibody was prepared using a quinone-modified protein as an immunogen to immunochemically detect the quinone on a protein. The established antibody recognized the quinone-NAC adduct, quinone-modified poly-L-lysine, and quinone-modified low-density lipoprotein. Quinone-modified proteins in human atherosclerotic lesions were immunohistochemically observed using the established antibody to the quinone and also a monoclonal antibody to tryptamine dione-modified protein, suggesting an occurrence of in vivo oxidation of serotonin and 5HIAA, accompanied by covalent adduction to biomolecules., Dec. 2016, 101, 500, 510, Scientific journal, True, 10.1016/j.freeradbiomed.2016.11.023

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• Non-coding RNA, CircRNAs and RNA-Binding Proteins Involved in the Pathogenesis of Cancers or Central Nervous System Disorders., Yuka Ikeda; Sae Morikawa; Moeka Nakashima; Sayuri Yoshikawa; Kurumi Taniguchi; Haruka Sawamura; Naoko Suga; Ai Tsuji; Satoru Matsuda, Circular RNAs (circRNAs), a newly recognized group of noncoding RNA transcripts, have established widespread attention due to their regulatory role in cell signaling. They are covalently closed noncoding RNAs that form a loop, and are typically generated during the splicing of precursor RNAs. CircRNAs are key post-transcriptional and post-translational regulators of gene expression programs that might influence cellular response and/or function. In particular, circRNAs have been considered to function as sponges of specific miRNA, regulating cellular processes at the post-transcription stage. Accumulating evidence has shown that the aberrant expression of circRNAs could play a key role in the pathogenesis of several diseases. Notably, circRNAs, microRNAs, and several RNA-binding proteins, including the antiproliferative (APRO) family proteins, could be indispensable gene modulators, which might be strongly linked to the occurrence of diseases. In addition, circRNAs have attracted general interest for their stability, abundance in the brain, and their capability to cross the blood-brain barrier. Here, we present the current findings and theragnostic potentials of circRNAs in several diseases. With this, we aim to provide new insights to support the development of novel diagnostic and/or therapeutic strategies for these diseases., 31 Mar. 2023, 9, 2, Scientific journal, True, 10.3390/ncrna9020023

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• Microorganisms, MDPI AG, Efficacy of Life Protection Probably from Newly Isolated Bacteria against Cisplatin-Induced Lethal Toxicity, Yuka Ikeda; Naoko Suga; Satoru Matsuda, Cisplatin may be commonly used in chemotherapy against various solid tumors. However, cisplatin has a limited safety range with serious side effects, which may be one of the dose-restraining reasons for cisplatin. A favorable therapeutic approach is immediately required for ameliorating cisplatin-induced toxicity. In the present study, the potential protective effects of certain bacteria have been investigated at the lethal dosage of cisplatin in mice experimental models. Treated under the highest dosage of cisplatin, treatment of certain commensal bacteria could significantly increase the survival rate. In addition, our findings revealed that probiotic supplementation of these bacteria could result in the attenuation of the damage appearance on the kidney as well as the alteration of several antioxidant-related gene expressions, including SOD1, SOD2, SOD3, Nrf2, and/or HO-1 genes in the high dosage of cisplatin-treated mice. In short, acute kidney injury in mice was induced by a single dose of cisplatin 11 or 15 mg/kg intraperitoneally. Then, peroral administration of newly isolated bacteria could protect against the cisplatin-induced injury, probably by decreasing oxidative stress. Therefore, the data shown here might suggest that the usage of certain probiotic supplementation could contribute to the life protection of patients suffering from severe toxicity of cisplatin. However, the molecular mechanisms need to be further explored., 06 Sep. 2023, 11, 9, 2246, 2246, Scientific journal, 10.3390/microorganisms11092246

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• Discovery medicine, Recent Progress of Chitosan Nanoparticles for the Development of Superior Delivery of Vaccines., Moeka Nakashima; Naoko Suga; Yuka Ikeda; Sayuri Yoshikawa; Satoru Matsuda, Chitosan seems to be an innovative biological material potentially utilized as a nanoparticle carrier for drug delivery, which could be low toxic, biocompatible, and easy to prepare. Chitosan nanoparticles have been employed in gene delivery. As a type of multifunctional adjuvant, chitosan nanoparticles could activate the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway to induce cell protection and/or proliferation via the modulation of autophagy within dendritic cells. In general, adjuvants may improve the innate and/or adaptive immune responses to a vaccine antigen by facilitating the antigen presentation of antigen presenting cells such as dendritic cells. The choice of a suitable adjuvant has become vital for improved safety and/or expanded application of vaccines. Fortunately, chitosan nanoparticles could be designed to target the dendritic cells to be enhanced by its adjuvant effect and for stimulating robust immune responses. Therefore, chitosan nanoparticles may be a good immune stimulant with encouraging properties for the development of superior vaccine delivery. Indeed, vaccines could play a key role in human health. In this review, we summarize the concept and/or recent progress in the field of chitosan nanoparticles, providing a valuable resource for investigating the molecular mechanisms of chitosan for the development of a greater vaccine., Mar. 2024, 36, 182, 457, 466, Scientific journal, True, 10.24976/Discov.Med.202436182.43

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• Biomolecules, Inspiring Tactics with the Improvement of Mitophagy and Redox Balance for the Development of Innovative Treatment against Polycystic Kidney Disease, Moeka Nakashima; Naoko Suga; Yuka Ikeda; Sayuri Yoshikawa; Satoru Matsuda, Polycystic kidney disease (PKD) is the most common genetic form of chronic kidney disease (CKD), and it involves the development of multiple kidney cysts. Not enough medical breakthroughs have been made against PKD, a condition which features regional hypoxia and activation of the hypoxia-inducible factor (HIF) pathway. The following pathology of CKD can severely instigate kidney damage and/or renal failure. Significant evidence verifies an imperative role for mitophagy in normal kidney physiology and the pathology of CKD and/or PKD. Mitophagy serves as important component of mitochondrial quality control by removing impaired/dysfunctional mitochondria from the cell to warrant redox homeostasis and sustain cell viability. Interestingly, treatment with the peroxisome proliferator-activated receptor-α (PPAR-α) agonist could reduce the pathology of PDK and might improve the renal function of the disease via the modulation of mitophagy, as well as the condition of gut microbiome. Suitable modulation of mitophagy might be a favorable tactic for the prevention and/or treatment of kidney diseases such as PKD and CKD., Feb. 2024, 14, 2, 10.3390/biom14020207

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• Non-coding RNA, Circular RNAs, Noncoding RNAs, and N6-methyladenosine Involved in the Development of MAFLD, Moeka Nakashima; Naoko Suga; Yuka Ikeda; Sayuri Yoshikawa; Satoru Matsuda, Noncoding RNAs (ncRNAs), including circular RNAs (circRNAs) and N6-methyladenosine (m6A), have been shown to play a critical role in the development of various diseases including obesity and metabolic disorder-associated fatty liver disease (MAFLD). Obesity is a chronic disease caused by excessive fat accumulation in the body, which has recently become more prevalent and is the foremost risk factor for MAFLD. Causes of obesity may involve the interaction of genetic, behavioral, and social factors. m6A RNA methylation might add a novel inspiration for understanding the development of obesity and MAFLD with post-transcriptional regulation of gene expression. In particular, circRNAs, microRNAs (miRNAs), and m6A might be implicated in the progression of MAFLD. Interestingly, m6A modification can modulate the translation, degradation, and other functions of ncRNAs. miRNAs/circRNAs can also modulate m6A modifications by affecting writers, erasers, and readers. In turn, ncRNAs could modulate the expression of m6A regulators in different ways. However, there is limited evidence on how these ncRNAs and m6A interact to affect the promotion of liver diseases. It seems that m6A can occur in DNA, RNA, and proteins that may be associated with several biological properties. This study provides a mechanistic understanding of the association of m6A modification and ncRNAs with liver diseases, especially for MAFLD. Comprehension of the association between m6A modification and ncRNAs may contribute to the development of treatment tactics for MAFLD., Feb. 2024, 10, 1, 10.3390/ncrna10010011

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• Genes, MDPI AG, Non-Coding RNAs and Gut Microbiota in the Pathogenesis of Cardiac Arrhythmias: The Latest Update, Naoko Suga; Yuka Ikeda; Sayuri Yoshikawa; Kurumi Taniguchi; Haruka Sawamura; Satoru Matsuda, Non-coding RNAs (ncRNAs) are indispensable for adjusting gene expression and genetic programming throughout development and for health as well as cardiovascular diseases. Cardiac arrhythmia is a frequent cardiovascular disease that has a complex pathology. Recent studies have shown that ncRNAs are also associated with cardiac arrhythmias. Many non-coding RNAs and/or genomes have been reported as genetic background for cardiac arrhythmias. In general, arrhythmias may be affected by several functional and structural changes in the myocardium of the heart. Therefore, ncRNAs might be indispensable regulators of gene expression in cardiomyocytes, which could play a dynamic role in regulating the stability of cardiac conduction and/or in the remodeling process. Although it remains almost unclear how ncRNAs regulate the expression of molecules for controlling cardiac conduction and/or the remodeling process, the gut microbiota and immune system within the intricate networks might be involved in the regulatory mechanisms. This study would discuss them and provide a research basis for ncRNA modulation, which might support the development of emerging innovative therapies against cardiac arrhythmias., 30 Aug. 2023, 14, 9, 1736, 1736, Scientific journal, 10.3390/genes14091736

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• Neurology International, MDPI AG, In Search of a Function for the N6-Methyladenosine in Epitranscriptome, Autophagy and Neurodegenerative Diseases, Naoko Suga; Yuka Ikeda; Sayuri Yoshikawa; Kurumi Taniguchi; Haruka Sawamura; Satoru Matsuda, Changes in epitranscriptome with N6-methyladenine (m6A) modification could be involved in the development of multiple diseases, which might be a prevalent modification of messenger RNAs (mRNAs) in eukaryotes. The m6A modification might be performed through the action of methyltransferases, demethylases, and methylation-binding proteins. Importantly, the m6A methylation may be associated with various neurological disorders including Alzheimer’s disease (AD), Parkinson’s disease (PD), depression, aging-related diseases, and/or aging itself. In addition, the m6A methylation might functionally regulate the eukaryotic transcriptome by influencing the splicing, export, subcellular localization, translation, stability, and decay of mRNAs. Neurodegenerative diseases may possess a wide variety of phenotypes, depending on the neurons that degenerate on occasion. Interestingly, an increasing amount of evidence has indicated that m6A modification could modulate the expression of autophagy-related genes and promote autophagy in neuronal cells. Oxidative stresses such as reactive oxygen species (ROS) could stimulate the m6A RNA methylation, which may also be related to the regulation of autophagy and/or the development of neurodegenerative diseases. Both m6A modification and autophagy could also play critical roles in regulating the health condition of neurons. Therefore, a comprehensive understanding of the m6A and autophagy relationship in human diseases may benefit in developing therapeutic strategies in the future. This paper reviews advances in the understanding of the regulatory mechanisms of m6A modification in the occurrence and development of neurodegenerative diseases and/or aging, discussing the possible therapeutic procedures related to mechanisms of m6A RNA methylation and autophagy., 10 Aug. 2023, 15, 3, 967, 979, Scientific journal, 10.3390/neurolint15030062

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• LWT, Elsevier BV, Thermal stability of cricket powder and its effects on antioxidant activity, physical, and sensory properties of rice crackers, Naoko Suga; Eri Tsumura; Yuzuka Naito; Ikue Hamaguchi; Satoru Matsuda; Kyuichi Kawabata; Kaoru Sakamoto, Aug. 2023, 186, 115267, 115267, Scientific journal, 10.1016/j.lwt.2023.115267

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• Exploration of Medicine, Roles of poly(ADP-ribose) polymerase 1 and mitophagy in progeroid syndromes as well as physiological ageing, Naoko Suga; Yuka Ikeda; Sayuri Yoshikawa; Satoru Matsuda, Progeroid syndromes are characterized by clinical signs of premature ageing, which may contain several diseases such as Werner syndrome, Bloom syndrome, Rothmund-Thomson syndrome, Hutchinson-Gilford progeria syndrome, and Cockayne syndrome. These disorders may also exhibit some pathological involvements reminiscent of primary mitochondrial diseases. Emerging evidence has linked mitochondria even to physiological ageing. In addition, alterations in the maintenance pathway of mitochondria have been also deliberated as relevant in age-related diseases. In particular, mitophagy and its regulatory pathway might be key process for the homeostasis of mitochondria. Therefore, chronic DNA damage and/or the activation of poly[adenosine diphosphate (ADP)-ribose] polymerase 1 (PARP1) could be a threat to the mitochondrial alterations. The PARP1 is an enzyme responding to the DNA damage, which might be also involved in the mitophagy. Interestingly, the PARP1 has been reported to play an important role in the longevity of lifespan, which has attracted growing attention with the social development. This review may provide a rationalized overview of the involvement of mitochondrial oxidative stresses in genetically defined accelerated ageing, progeroid syndromes, physiological ageing, and/or age-related diseases for the innovative therapeutic approaches., 2023, 4, 5, 822, 838, 10.37349/emed.2023.00180

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• Genes, Potential Molecular Mechanisms of Alcohol Use Disorder with Non-Coding RNAs and Gut Microbiota for the Development of Superior Therapeutic Application., Moeka Nakashima; Naoko Suga; Sayuri Yoshikawa; Yuka Ikeda; Satoru Matsuda, Many investigations have evaluated the expression of noncoding RNAs (ncRNAs) as well as their related molecular functions and biological machineries in individuals with alcohol dependence. Alcohol dependence may be one of the most prevailing psychological disorders globally, and its pathogenesis is intricate and inadequately comprehended. There is substantial evidence indicating significant links between multiple genetic factors and the development of alcohol dependence. In particular, the critical roles of ncRNAs have been emphasized in the pathology of mental illnesses, probably including alcohol dependence. In the comprehension of the action of ncRNAs and their machineries of modification, furthermore, they have emerged as therapeutic targets for a variety of psychiatric illnesses, including alcohol dependence. It is worth mentioning that the dysregulated expression of ncRNAs has been regularly detected in individuals with alcohol dependence. An in-depth knowledge of the roles of ncRNAs and m6A modification may be valuable for the development of a novel treatment against alcohol dependence. In general, a more profound understanding of the practical roles of ncRNAs might make important contributions to the precise diagnosis and/or actual management of alcohol dependence. Here, in this review, we mostly focused on up-to-date knowledge regarding alterations and/or modifications in the expression of ncRNAs in individuals with alcohol dependence. Then, we present prospects for future research and therapeutic applications with a novel concept of the engram system., 29 Mar. 2024, 15, 4, Scientific journal, True, 10.3390/genes15040431

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MISC

• Abstracts of the Annual Meeting of the Japan Society of Cookery Science, The Japan Society of Cookery Science, The study on brown rice processing to enhance the prebiotic potential, Suga Naoko; Nuka Erika; Uchida Haruka; Kawabata Kyuichi; Sakamoto Kaoru, 【目的】玄米は、食物繊維を多く含むことから、プレバイオティクス作用が期待されている食品の一つである。しかし、玄米の食物繊維の多くは不溶性であり、善玉菌に対する資化性を高めるには、それらを水溶化させる必要がある。そこで本研究では、玄米のプレバイオティクス性を高める調理加工方法を明らかにすることを目的として、加工による機能性成分の変動と食物繊維の一つであるアラビノキシランの水溶化について検討した。 【方法】玄米を原料として、穀物膨張機によりパフ加工したサンプルを二種、また焙煎機により焙煎したサンプルを二種作製した。機能性成分量の評価として、抗酸化性はDPPHラジカル消去活性で評価するとともに、フォーリンチオカルト法により総ポリフェノール含量を測定した。また、フロログルシノール法を用いて総アラビノキシラン及び水溶性アラビノキシランの定量を行った。さらに、糠層ではアラビノキシランがポリフェノールの一種であるフェルラ酸と結合して存在していることから、高速液体クロマトグラフを用いて総フェルラ酸及び遊離フェルラ酸量について測定した。 【結果・考察】未加工の玄米と比較して、パフ加工および焙煎加工したものは抗酸化活性および総ポリフェノール量が増加することを確認した。興味深いことに、水溶性アラビノキシラン量と遊離フェルラ酸量は、焙煎加工したサンプルにおいては減少したが、パフ加工したサンプルにおいては顕著な増加が認められた。高い圧力下でパフ加工したサンプルにおいては、総アラビノキシランと総フェルラ酸がともに減少したが、アラビノキシランを顕著に水溶化したことから、パフ加工はプレバイオティクス効果を高めるのに適した加工方法であることが示唆された。, Sep. 2022, 33, 8, 10.11402/ajscs.33.0_8

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• Abstracts of the Annual Meeting of the Japan Society of Cookery Science, The Japan Society of Cookery Science, Cooking properties and antioxidative activity of rice crackers with cricket powder, Tsumura Eri; Naito Yuzuka; Sakamoto Kaoru; Suga Naoko, 【目的】昆虫食を普及するためには、嗜好性の向上や高付加価値化に寄与する知見を増やす必要がある。そこで、本研究では、食用コウロギパウダー（以下、CP）をライスクラッカーに添加した際の物性や色と抗酸化性に与える影響ついて検証した。 【方法】CPはTAKEO社のものを使用した。初めに、CPのみを140、160、180℃で30分間加熱し、DPPHラジカル捕捉活性およびSOD様活性を測定することで、CPが有する抗酸化力の熱安定性について評価した。続いて、米粉にCPをそれぞれ0、10、20、30 wt%添加したライスクラッカーを作成した。ライスクラッカーの物性評価として破断荷重を、外観評価として明度（L*）と色度（a*,b*）をそれぞれ測定した。またライスクラッカーの抗酸化力についてもCPと同様にして評価した。 【結果・考察】加熱したCPのDPPHラジカル捕捉活性は、未加熱のものと比較して減少したが、その減少率は大きくなかった。一方で、SOD様活性については加熱により高くなる傾向を示した。また、CPを添加したライスクラッカーにおいては、添加なしのものと比較して破断荷重が低下し、暗く赤みが増すことが確認された。さらに、CPを添加したライスクラッカーでは、DPPHラジカル捕捉活性及びSOD様活性がともに高くなった。, Sep. 2022, 33, 173, 10.11402/ajscs.33.0_173

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Books etc

• 978-4-06-534135-3

Presentations

• 菅尚子, 額惠理香, 内田はるか, 川畑球一, 坂本薫, 日本調理科学会 2022年度大会, プレバイオティクス性の向上を目指した玄米加工に関する研究, Sep. 2022
• 津村恵莉, 内藤柚子香, 坂本薫, 菅尚子, 日本調理科学会 2022年度大会, コオロギパウダーを添加したライスクラッカーの調理特性および抗酸化性, Sep. 2022
• 菅尚子, 村上明, 有満秀幸, 塩竈和也, 田中更沙, 伊藤美紀子, 加藤陽二, 第74回日本酸化ストレス学会学術集会, 大腸炎モデルマウスにおけるセロトニン酸化物Tryptamine-4,5-dioneの定量と局在性解析, May 2021
• Naoko Suga, Hideyuki Arimitsu, Akira Murakami, Yoji Kato, The 7th International Conference on Food Factors, Suppressive effects of luteolin on serotonin production in RBL-2H3 cells, Dec. 2019
• Yoji Kato, Sae Fujisjima, Naoko Suga, Aoi Sugimoto,  Makoto Naoi, Wakako Maruyama, The 7th International Conference on Food Factors, Irreversible inhibition  of monoamine oxidases by serotonin-derived quinones, Dec. 2019
• Yui Kishi, Masaki Kawai, Yayako Okano, Naoko Suga, Mai Kato, Akika Nagata, Noriyuki Miyoshi, Yoji Kato, The 7th International Conference on Food Factors, Loss of key components, methylglyoxal and 2′-methoxyacetophenone, in manuka honey by heat processing, Dec. 2019
• Naoko Suga, Akira Murakami, Yoji Kato, The 9th Biennial Meeting of Society for Free Radical Research-Asia, Oxidation of serotonin metabolite, 5-hydroxyindoleacetic acid, in the intestinal mucosa - A model study using intestine homogenate -, Apr. 2019
• 菅尚子、岡野やや子、河合翔太、坂本 薫、加藤陽二, 日本調理科学会近畿支部第44回研究発表会, マヌカ蜂蜜特有成分の熱安定性, Dec. 2018
• 岡野やや子、河合翔太、清水与代、菅尚子、加藤陽二, 第23回日本フードファクター（JSoFF）学会学術集会, マヌカ蜂蜜に含まれるケミカルマーカーの熱安定性について, Sep. 2018
• 菅尚子、村上明、有満秀幸、伊藤美紀子、加藤陽二, 第71回日本酸化ストレス学会学術集会, 腸粘膜におけるTryptamine-4,5-dione (TD) の動態解析−タンパク質修飾と遊離TD検出−, May 2018
• 森田朱美、朝鍋けいと、菅尚子、有満秀幸、加藤陽二, 第22回日本フードファクター（JSoFF）学会学術集会, 生理的条件下におけるセロトニン及びその代謝物に由来するキノン体生成～NETs形成との関連～, Dec. 2017
• 菅尚子、村上明、中村宜督、石坂朱里、北元憲利、伊藤美紀子、加藤陽二, 第22回フードサイエンスフォーラム（FSF）学術集会, セロトニン酸化物Tryptamine-4,5-dioneによる神経細胞毒性と保護効果, Sep. 2016
• 菅尚子、村上明、中村宜督、石坂朱里、北元憲利、伊藤美紀子、加藤陽二, 第69回日本酸化ストレス学会学術集会, セロトニン由来キノン体Tryptamine-4,5-dioneによる神経細胞毒性と保護効果, Aug. 2016
• Naoko Suga, Akari Ishisaka, Noritoshi Kitamoto, Mikiko Ito, Akira Murakami, Yoshimasa Nakamura, Yoji Kato, The Society for Free Radical Research Australasia & Japan 7th Joint Meeting, Modification of cellular proteins and induction of self-defense genes expressions by tryptamine-4,5-dione, a serotonin oxidation product, Dec. 2015
• Naoko Suga, Akari Ishisaka, Noritoshi Kitamoto, Akira Murakami, Yoshimasa Nakamura, Yoji Kato, The 6th International Conference on Food Factor, Effect of quinone derived from 5-hydroxytryptamine on expression of genes in SH-SY5Y neuroblastoma cells, Nov. 2015
• 菅尚子、石坂朱里、北元憲利、中村宜督、加藤陽二, 第68回日本酸化ストレス学会学術集会, セロトニン由来キノン体による細胞タンパク質修飾及び遺伝子発現に与える影響, Jun. 2015
• 菅尚子、石坂朱里、北元憲利、中村宜督、加藤陽二, 日本農芸化学会2015年度大会, セロトニン由来キノン体のヒトLDL修飾と細胞への影響, Mar. 2015
• 菅尚子、小野成輝、石坂朱里、北元憲利、加藤陽二, 第19回日本フードファクター（JSoFF）学会学術集会, 抗酸化物質によるセロトニン由来キノン体タンパク質の修飾阻害, Nov. 2014
• 菅尚子、小野成輝,石坂朱里,北元憲利, Tony Kettle,加藤陽二, 第67回日本酸化ストレス学会学術集会, セロトニン関連物質に由来するキノン体による細胞内チオールへの影響, Sep. 2014

Awards

• 第44回研究発表会 若手優秀発表賞, 日本調理科学会近畿支部, Dec. 2018
• 院生＆若手セミナーバトル 金賞, 第22回フードサイエンスフォーラム学術集会, Sep. 2016
• 若手研究奨励賞, 日本酸化ストレス学会, Dec. 2015
• Young Scientist Oral Presentation Award, The Society for Free Radical Research Australasia, Dec. 2015
• 若手研究奨励賞, 日本フードファクター学会（JSoFF）, Nov. 2015
• Poster Award, International Conference on Food Factors（ICoFF）, Nov. 2015

Research Projects

• 若手研究, 01 Apr. 2022, 31 Mar. 2025, 22K13604, 食物アレルギー反応の低減を目指したプロバイオティクス発酵法の設計, 菅 尚子, 日本学術振興会, 科学研究費助成事業 若手研究, 甲南女子大学, 4680000, 3600000, 1080000, kaken

  対象リソースIRI
• 研究活動スタート支援, 30 Aug. 2021, 31 Mar. 2023, 21K20201, プレバイオティクス効果の向上を目指した玄米加工に関する研究, 菅 尚子, 日本学術振興会, 科学研究費助成事業 研究活動スタート支援, 甲南女子大学, 1430000, 1100000, 330000, 玄米を原料として、時間と圧力を変えて穀物膨張機によりパフ加工したサンプルを二種、また時間を調整して焙煎機により焙煎したサンプルを二種作製した。 未加工の玄米と比較して、パフ加工および焙煎加工したものは抗酸化活性および総ポリフェノール量が増加することを確認した。さらに、フロログルシノール法を用いてアラビノキシランの定量を行った。この結果、より高い圧力下でパフ加工したサンプルにおいて、水溶性アラビノキシラン量は未加工の玄米と比較して顕著に増加することを確認した。また、同サンプルは、総フェルラ酸量が10%程度減少したが、水溶性フェルラ酸量は顕著に増加した。一方で、焙煎加工したサンプルでは、水溶性アラビノキシラン量および遊離フェルラ酸量が減少する傾向が認められた。 膨化加工によって不溶性のアラビノキシランの一部が水溶化したことから、膨化加工はプレバイオティクス効果を高めるのに適した加工である可能性が示唆された。今後は、膨化加工した玄米サンプルを用いて、プロバイオティクス菌の増殖や代謝物に与える影響について検証していく。, kaken

  対象リソースIRI