Researchers Database

YASUDA Keiko

    Vice President Vice President
    Faculty Division of Natural Sciences Research Group of Biological Sciences Professor
    Organization for the Promotion of Gender Equallity Director, Organization for the Promotion of Gender Equallity
Contact:
ponkocc.nara-wu.ac.jp
Last Updated :2021/10/23

researchmap

Degree

  • (BLANK), Kyoto University
  • (BLANK), Nara Women's University

Research Interests

  • reproductive endocrinology ovary testis 

Research Areas

  • Life sciences, Animals: biochemistry, physiology, behavioral science

Research Experience

  • Jan. 2016, 教授
  • Apr. 2013 Dec. - 2015, 准教授
  • Apr. 1996 Mar. - 2013, 講師
  • Apr. 1994 Mar. - 1996, 助手
  • Apr. 2020, Nara Women's University, 副学長(男女共同参画担当)

Education

  • - 1982, Nara Women's University, Graduate School, Division of Natural Science, 生物学
  • - 1980, Nara Women's University, Faculty of Science, 生物学

Committee Memberships

  • 日本動物学会近畿支部委員

Published Papers

  • Carbohydrate-Appended TQNPEN [N,N,N′,N′-Tetrakis(2-quinolylmethyl)-3-aza-1,5-pentanediamine] Derivatives for Fluorescence Detection of Intracellular Cd2+

    Yuji Mikata; Kana Nozaki; Minori Kaneda; Keiko Yasuda; Masato Aoyama; Satoshi Tamotsu; Arimasa Matsumoto

    © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Invited for the cover of this issue is the group of Yuji Mikata from Nara Women's University, Japan. The cover image shows the application of a carbohydrate-appended polyquinoline ligand as a fluorescent sensor for intracellular Cd2+., 29 Jun. 2018, European Journal of Inorganic Chemistry, 2018 (24), 2731, doi;scopus;scopus_citedby

  • Replacement of quinolines with isoquinolines affords target metal ion switching from Zn2+ to Cd2+ in the fluorescent sensor TQLN (N,N,N ',N '-tetrakis(2-quinolylmethyl)-2,6-bis(aminomethyl) pyridine)

    Yuji Mikata; Ayaka Takekoshi; Minori Kaneda; Hideo Konno; Keiko Yasuda; Masato Aoyama; Satoshi Tamotsu

    A quinoline-based heptadentate ligand, N, N,N',N'-tetrakis(2-quinolylmethyl)- 2,6-bis(aminomethyl) pyridine (TQLN), exhibits a Zn2+ -specific fluorescence increase at 428 nm, which is assigned to excimer emission (I-Zn/I-o = 38, I-Cd/I-Zn = 24%,phi(Zn) = 0.069). In contrast, the isoquinoline counterpart 1-isoTQLN exhibits a Cd2+ -specific fluorescence increase at 365 nm attributable to monomer emission (I-Cd/I-o = 83, I-Zn/I-Cd = 19%,phi(Cd) = 0.015)., ROYAL SOC CHEMISTRY, Jan. 2017, DALTON TRANSACTIONS, 46 (3), 632 - 637, doi;web_of_science

    Scientific journal

  • The protein phosphatase 6 catalytic subunit (Ppp6c) is indispensable for proper post-implantation embryogenesis

    Honami Ogoh; Nobuhiro Tanuma; Yasuhisa Matsui; Natsuki Hayakawa; Ayaka Inagaki; Mami Sumiyoshi; Yuki Momoi; Ayako Kishimoto; Mai Suzuki; Nozomi Sasaki; Tsukasa Ohuchi; Miyuki Nomura; Yuriko Teruya; Keiko Yasuda; Toshio Watanabe; Hiroshi Shima

    Ppp6c, which encodes the catalytic subunit of phosphoprotein phosphatase 6 (PP6), is conserved among eukaryotes from yeast to humans. In mammalian cells, PP6 targets I kappa B epsilon for degradation, activates DNA-dependent protein kinase to trigger DNA repair, and is reportedly required for normal mitosis. Recently, Ppp6c mutations were identified as candidate drivers of melanoma and skin cancer. Nonetheless, little is known about the physiological role of Ppp6c. To investigate this function in vivo, we established mice lacking the Ppp6c phosphatase domain by crossing heterozygous mutants. No viable homozygous pups were born, indicative of a lethal mutation. Ppp6c homozygous mutant embryos were identified among blastocysts, which exhibited a normal appearance, but embryos degenerated by E7.5 and showed clear developmental defects at E8.5, suggesting that mutant embryos die after implantation. Accordingly, homozygous blastocysts showed significant growth failure of the inner cellmass (ICM) in in vitro blastocyst culture, and primary Ppp6c exon4-deficient MEFs showed greatly reduced proliferation. These results establish for the first time that the Ppp6c phosphatase domain is indispensable for mouse embryogenesis after implantation. (C) 2016 Elsevier Ireland Ltd. All rights reserved., ELSEVIER SCIENCE BV, Feb. 2016, MECHANISMS OF DEVELOPMENT, 139, 1 - 9, doi;web_of_science

    Scientific journal

  • Dwarf males in the epizoic barnacle Octolasmis unguisiformis and their implications for sexual system evolution

    Kota Sawada; Ryuta Yoshida; Keiko Yasuda; Sachi Yamaguchi; Yoichi Yusa

    Descriptions of the diversity of sexual systems in animal taxa such as the thoracican barnacles are needed to study the evolution of sexual systems. Androdioecious systems (coexistence of hermaphrodites and males) are particularly important due to their possible role as evolutionary intermediates in transitions between hermaphroditism and dioecy. In this study, we used histology to examine the sexual system of the crab-epizoic barnacle Octolasmis unguisiformis to determine if dwarf males were present or not; a previous study reported the existence of conspecific-attached individuals, but did not investigate their sexuality. All conspecific-attached individuals were dwarf males, irrespective of their attachment site. However, crab-attached individuals never acted as dwarf males even if they were small and lived together with large individuals. The result emphasizes the importance of attachment to conspecifics, but not to specific sites on conspecifics, in the evolution of dwarf males. Other individuals were hermaphroditic, indicating androdioecy in this species. However, their functional sexuality (that is, whether they actually do act as males) requires further study. The presence of dwarf males in this species supports theoretical predictions that small group size, short-lived habitats, or spatial limitation favor the evolution of dwarf males., WILEY-BLACKWELL, Jun. 2015, INVERTEBRATE BIOLOGY, 134 (2), 162 - 167, doi;web_of_science

    Scientific journal

  • Sexual system of a symbiotic pedunculate barnacle Poecilasma kaempferi (Cirripedia: Thoracica)

    Sachi Yamaguchi; Sachi Yoshida; Atsushi Kaneko; Kota Sawada; Keiko Yasuda; Yoichi Yusa

    The pedunculate barnacle Poecilasma kaempferi lives on the body surface of deep-sea decapod crustaceans. We observed that some individuals of this species are attached to conspecifics and investigated their reproductive status to determine whether such individuals are functional dwarf males, as reported for other pedunculates. We also experimentally evaluated the effect of attachment site (whether directly attached to the substratum, attached to conspecifics, or in contact with conspecifics) on reproductive status in an aquarium experiment. Both approaches demonstrated that in this species, conspecific-attached individuals are not dwarf males and are indistinguishable from other individuals in terms of reproductive status. Thus, this species should be considered hermaphroditic rather than androdioecious (i.e. males + hermaphrodites). Such conspecific-attached hermaphrodites may represent an example of pre-adaptation for the evolution of dwarf males. © 2014 Taylor & Francis., Taylor and Francis Ltd., 2014, Marine Biology Research, 10 (6), 635 - 640, doi

    Scientific journal

  • 8-TQEN (N,N,N ',N '-tetrakis(8-quinolylmethyl)ethylenediamine) analogs as fluorescent cadmium sensors: strategies to enhance Cd2+-induced fluorescence and Cd2+/Zn2+ selectivity

    Yuji Mikata; Ayaka Takekoshi; Asako Kizu; Yuki Nodomi; Masato Aoyama; Keiko Yasuda; Satoshi Tamotsu; Hideo Konno; Shawn C. Burdette

    In order to exploit the untapped sensing potential of the TQEN (N,N,N',N'-tetrakis(2-quinolylmethyl)ethylenediamine) family of fluorescent probes, 8-TQEN (N,N,N',N'-tetrakis(8-quinolylmethyl)ethylenediamine) analogs were designed and characterized. Although 8-TQEN lacks practicality owing to poor solubility in both aqueous media and organic solvents, 6-MeO-8-TQEN (N,N,N',N'-tetrakis(6-methoxy-8-quinolylmethyl) ethylenediamine, 1b) exhibits Cd2+-selective fluorescence enhancement at 395 nm in DMF-HEPES buffer (1 : 1), (I-Cd/I-0 = 25, lambda(ex) = 332 nm, phi(Cd) = 0.025). Zn2+ induces weaker fluorescence (I-Zn/I-0 = 9, I-Zn/I-Cd = 0.35). In contrast, both the parent probes TQEN (I-Zn/I-0 = 23, I-Cd/I-Zn = 0.64) and 6-MeOTQEN (I-Zn/I-0 = 11, I-Cd/I-Zn = 1.29 exhibit higher sensitivity toward Zn2+. When the quinoline groups were replaced with 8-hydroxyquinoline different responses were observed. The propanediamine derivative, 8-TQOEPN (N,N,N',N'-tetrakis(8-quinolyloxyethylene)propanediamine, 2c) exhibits significant fluorescence enhancement at 423 nm upon Cd2+ binding (lambda(ex) = 325 nm, I-Cd/I-0 = 19, phi(Cd) = 0.31). Fluorescence enhancement is Cd2+-specific as Zn2+ induces only more modest emission increases (I-Zn/I-0 = 8.8, I-Zn/I-Cd = 0.45)., ROYAL SOC CHEMISTRY, 2014, RSC ADVANCES, 4 (25), 12849 - 12856, doi;web_of_science

    Scientific journal

  • Isoquinoline-derivatized tris(2-pyridylmethyl)-amines as fluorescent zinc sensors with strict Zn2+/Cd2+ selectivity

    Yuji Mikata; Keiko Kawata; Saaya Takeuchi; Kaori Nakanishi; Hideo Konno; Saori Itami; Keiko Yasuda; Satoshi Tamotsu; Shawn C. Burdette

    Tris(2-pyridylmethyl)amine-based fluorescent ligands, N,N-bis(1-isoquinolylmethyl)-2-pyridylmethyl-amine (1-isoBQPA) and N,N-bis(7-methoxy-1-isoquinolylmethyl)-2-pyridylmethylamine (7-MeO-1-isoBQPA), have been prepared and the Zn2+-induced fluorescence enhancement has been investigated. Upon excitation at 324 nm, 1-isoBQPA exhibits a very weak emission (phi = similar to 0.010) in DMF-H2O (1 : 1). Upon Zn2+ addition, the 1-isoBQPA fluorescence increases (phi(Zn) = 0.055) at 357 nm and 464 nm. The fluorescence enhancement at longer wavelengths is Zn2+-specific, whereas Cd2+ induces a small emission increase at 464 nm (I-Cd/I-0 = 1.1, I-Cd/I-Zn = 14%). The Zn2+/Cd2+ selectivity of the fluorescent response correlates with the Cd-N-isoquinoline and Zn-N-isoquinoline bond distances measured in the crystal structures. Introduction of methoxy groups into the 1-isoBQPA chromophore enhances the fluorescence significantly (phi(Zn) = 0.213), which affords 7-MeO-1-isoBQPA properties amenable for fluorescence microscopy in living cells., ROYAL SOC CHEMISTRY, 2014, DALTON TRANSACTIONS, 43 (28), 10751 - 10759, doi;web_of_science

    Scientific journal

  • Theca Cell Layer Formation in Mouse Ovarian Follicle Culture in vitro

    Saori Itami; Keiko Yasuda; Satoshi Tamotsu; Atsushi Sakai

    UNIV TOKYO CYTOLOGIA, Sep. 2012, CYTOLOGIA, 77 (3), 288 - 288, web_of_science

    Scientific journal

  • Synthesis of Rhenium(I) Tricarbonyl Complexes with Carbohydrate-Pendant Tridentate Ligands and Their Cellular Uptake

    Yuji Mikata; Kyoko Takahashi; Yuka Noguchi; Masami Naemura; Anna Ugai; Saori Itami; Keiko Yasuda; Satoshi Tamotsu; Takashi Matsuo; Tim Storr

    Twelve [ReIL(CO)(3)](n+) complexes with various carbohydrate-pendant ligands L have been prepared and their uptake into HeLa S3 cells were investigated. The ligand library includes: (i) glucose/galactose as the carbohydrate group; (ii) bis(2-pyridylmethyl) amine (DPA), bis(2-quinolylmethyl)amine (DQA), or N-(2-pyridylmethyl)glycine (NPG) as the metal binding component; and (iii) an ethylene chain as a linker between the metal binding site and the O/C-glycosides. Microwave induced plasma mass spectroscopy (MIP-MS) measurements revealed that all complexes were extensively incorporated into the HeLa cells over a 24 h period, and the DQA complexes showed the highest uptake of all the complexes in the series. However, in comparison to the corresponding Re complexes without the pendant carbohydrate functions (prepared with the related ligands LDPA, LDQA, and L-NPG), only the NPG complexes exhibited carbohydrate enhanced cellular uptake. Considering their water solubility and cellular uptake properties, the NPG complexes containing an O-glycoside group (L1 and L'1) are the best candidates for enhancing cellular uptake of metal ions. Microscopic analysis with PC-12 cells in the presence of the fluorescent complex [Re(L'7)(CO)(3)]Cl, revealed that the complex stays in the cell cytosol and cannot penetrate into the nucleus., WILEY-V C H VERLAG GMBH, Jan. 2012, EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2012 (2), 217 - 225, doi;web_of_science

    Scientific journal

  • Quinoline-Based, Glucose-Pendant Fluorescent Zinc Probes

    Yuji Mikata; Anna Ugai; Keiko Yasuda; Saori Itami; Satoshi Tamotsu; Hideo Konno; Satoshi Iwatsuki

    Quinoline-based tetradentate ligands with glucose pendants, N,N'-bis[2-(beta-d-glucopyranosyloxy)ethyl]-N,N'-bis[(6-methoxyquinolin-2-yl)methyl]ethylenediamine (N,N'-6-MeOBQBGEN) and its N,N-counterpart, N,N-6-MeOBQBGEN, have been prepared, and their fluorescence-spectral changes upon Zn binding were investigated. Upon excitation at 336 nm, N,N'-6-MeOBQBGEN showed weak fluorescence (?approximate to 0.016) in HEPES buffer (HEPES 50 mM, KCl 100 mM, pH 7.5). In the presence of Zn, N,N'-6-MeOBQBGEN exhibited a significant increase in fluorescence (?=0.096) at 414 nm. The fluorescence enhancement is specific for Zn and Cd (ICd/IZn of 50% at 414 nm). On the other hand, N,N-6-MeOBQBGEN exhibited a smaller fluorescence enhancement upon Zn complexation (?=0.043, ?ex=334 nm, ?em=407 nm) compared with N,N'-6-MeOBQBGEN. Fluorescence microscopic analysis using PC-12 rat adrenal cells revealed that N,N'-6-MeOBQBGEN exhibits a 1.8-fold higher fluorescence-signal response to Zn ion concentration compared with sugar-depleted compound 2 (N,N'-bis[(6-methoxyquinolin-2-yl)methyl]ethylenediamine), due to its enhanced uptake into cells due to the targeting ability of the attached carbohydrates., WILEY-V C H VERLAG GMBH, Sep. 2012, CHEMISTRY & BIODIVERSITY, 9 (9), 2064 - 2075, doi;web_of_science

    Scientific journal

  • The maize coleoptiles do not perform typical C4 photosynthesis: investigation \nwith special reference to anatomy, photosynthetic property, and gene \nexpression.

    YASUDA Keiko; Fukaya Y; Yoshioka N; Sakakibara H; Andro CS; Mae T; Sakai A

    2012, Plant Physiology, 24, 111-121

  • Co-culturing of follicles with interstitial cells in collagen gel reproduce follicular development accompanied with theca cell layer formation

    Saori Itami; Keiko Yasuda; Yuka Yoshida; Chiyuki Matsui; Sachie Hashiura; Atsushi Sakai; Satoshi Tamotsu

    Background: The mechanism of theca cell layer formation in mammalian ovaries has not been elucidated; one reason is that there is no follicle culture system that can reproduce theca cell layer formation in vitro. Therefore, a three-dimensional follicle culture system that can reproduce theca cell layer formation is required. Methods: A collagen gel was used in the follicle culture system. To determine the optimum conditions for follicle culture that can reproduce theca cell layer formation, the effects of hormonal treatment and cell types co-cultured with follicles were examined. In addition, immunohistochemistry was used to examine the properties of the cell layers formed in the outermost part of follicles. Results: Follicles maintained a three-dimensional shape and grew in collagen gel. By adding follicle-stimulating hormone (FSH) and co-culturing with interstitial cells, the follicles grew well, and cell layers were formed in the outermost part of follicles. Immunohistochemistry confirmed that the cells forming the outermost layers of the follicles were theca cells. Conclusion: In this study, follicle culture system that can reproduce theca cell layer formation in vitro was established. In our opinion, this system is suitable for the analysis of theca cell layer formation and contributes to our understanding of the mechanisms of folliculogenesis., BIOMED CENTRAL LTD, Dec. 2011, REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 9, 159-167, doi;web_of_science

    Scientific journal

  • The roles of Thy-1 and integrin beta3 in cell adhesion during theca cell layer formation, and the effect of \nfollicle-stimulating hormone on Thy-1 and integrin beta3 localization in mouse ovarian follicles.

    YASUDA Keiko; Saori Itami; Satoshi Tamotsu; Atsushi Sakai; Keiko Yasuda

    Jan. 2011, Biology of Reproduction, 84, 986-995

  • Methoxy-substituted isoTQEN family for enhanced fluorescence response toward zinc ion

    Yuji Mikata; Azusa Yamashita; Keiko Kawata; Hideo Konno; Saori Itami; Keiko Yasuda; Satoshi Tamotsu

    Previously, we have reported that 1- and 3-isoTQENs (N, N, N', N'-tetrakis(1- or 3-isoquinolylmethyl)ethylenediamines) exhibit a specific fluorescence enhancement toward zinc ion. In this study, three methoxy-substituted derivatives of 1-isoTQEN were synthesized and their fluorescent response toward zinc ion was studied. The substitution pattern of the methoxy group significantly changes the solubility of compounds in aqueous DMF, lambda(max) in the absorption spectra, excitation/emission wavelengths and fluorescence intensity of zinc complexes. In the presence of zinc ion, 7-MeO-1-isoTQEN exhibits higher fluorescence intensity and longer excitation/emission wavelengths (lambda(ex) = 342 nm, lambda(em) = 526 nm) than 6-MeO-1-isoTQEN (lambda(ex) = 303 nm, lambda(em) = 469 nm) and 5,6,7-triMeO-1-isoTQEN (lambda(ex) = 340 nm, lambda(em) = 504 nm). The fluorescence intensity of a zinc complex of 7-MeO-1-isoTQEN (phi = 0.122) is four times higher than the parent 1-isoTQEN (phi = 0.034) under the same conditions. The crystal structure of 7-MeO-1-isoTQEN-Zn complex reveals that all six nitrogen atoms participate to the metal coordination with ideal octahedral geometry, affording significantly high metal binding affinity comparable with TPEN (N, N, N', N'-tetrakis(2-pyridylmethyl)ethylenediamine). 7-MeO-1-isoTQEN detects zinc ion concentration change in cells by fluorescence microscopic analysis., ROYAL SOC CHEMISTRY, 2011, DALTON TRANSACTIONS, 40 (16), 4059 - 4066, doi;web_of_science

    Scientific journal

  • Methoxyquinoline-diethylenetriamine conjugate as a fluorescent zinc sensor

    Yuji Mikata; Azusa Yamashita; Keiko Kawata; Hideo Konno; Saori Itami; Keiko Yasuda; Satoshi Tamotsu

    A 6-methoxyquinoline conjugated diethylenetriamine derivative, N,N ''-bis(6-methoxy-2-quinolylmethyl) diethylenetriamine (6-MeOBQDIEN) has been synthesized and its fluorescent response toward zinc ion was investigated. In the presence of zinc ion, 6-MeOBQDIEN exhibits fluorescence (lambda(ex) = 329 nm, lambda(em) = 418 nm, phi = 0.039). The fluorescent intensity of the zinc complex of the compound is two times higher than the parent BQDIEN (phi = 0.021) under the same conditions. The crystal structure of 6-MeOBQDIEN-Zn complex shows that all five nitrogen atoms participate to the metal coordination in a distorted square-pyramidal geometry (tau = 0.145) with the aliphatic nitrogen in an apical position. Fluorescent microscopic analysis using 6-MeOBQDIEN reveals the zinc ion concentration change in living cells., ROYAL SOC CHEMISTRY, 2011, DALTON TRANSACTIONS, 40 (18), 4976 - 4981, doi;web_of_science

    Scientific journal

  • Development of single shot soft x-ray contact microscopy system for nano-scale dynamics measurement of living biological specimen

    Maki Kishimoto; Masataka Kado; Masahiko Ishino; Satoshi Tamotsu; Keiko Yasuda; Kunio Shinohara

    We have been developing a picosecond single shot soft x-ray contact microscopy system for observing the nanometer-scale inner structure of the living biological specimen in a hydrated condition. The microscopy system consists of an intense IR pump laser system for generating laser-induced plasma as a soft x-ray source and x-ray microscope chamber. The pump laser system employs OPCPA (Optical Parametric Chirped Pulse Amplification) technique to obtain a high contrast pump laser pulse, and we can generate water-window x-rays effectively by combining it to an ultra-thin metal target. The x-ray microscope chamber is composed of a vacuum chamber, a focusing lens, a metal film target, an in-vacuum type sample holder. The pump laser pulse is focused on the metal film target with a focusing lens. The soft x-rays from the laser-induced plasma illuminates bio-specimens on the PMMA photo resist set in the in-vacuum sample holder. The photo resist is developed and the x-ray transmission image recorded on the photo resist is read out by AFM. We took x-ray images of hydrated Leydig cells from mouse testicle and demonstrated that the developed x-ray microscopy system has a spatial resolution of about 100 nm., AMER INST PHYSICS, 2012, LASER-DRIVEN RELATIVISTIC PLASMAS APPLIED TO SCIENCE, ENERGY, INDUSTRY, AND MEDICINE, 1465, 43 - 47, doi;web_of_science

    International conference proceedings

  • Observation of actin filaments in Leydig cells with a contact-type soft x-ray microscope with laser plasma x-ray source.

    YASUDA Keiko; M. Kado; M. Ishino; S. Tamotsu; K. Yasuda; M. Kishimoto; M. Nishikino; Y. Kinjo; K. Shinohara

    2010, IEEJ Trans, 130 (1774-1778)

  • Effects of chloroplast dysfunction in subpopulation of leaf mesophyll cells on photosynthetic and respiratory activities of a whole leaf: A preliminary study using variegated leaves of Hedera helix L.

    YASUDA Keiko; Naoko Yoshioka; Yuki Imanishi; Keiko Yasuda; Atsushi Sakai

    2009, plant Morphology, 21 (1), 87-91

  • Changes in the distribution of tenascin and fibronectin in the mouse ovary during folliculogenesis, atresia, corpus luteum formation and luteolysis

    K Yasuda; E Hagiwara; A Takeuchi; C Mukai; C Matsui; A Sakai; S Tamotsu

    Tenascin and fibronectin are components of the extracellular matrices that oppose and promote adhesion, respectively. Using immunohistochemical techniques, we studied the distribution of tenascin and fibronectin in the mouse ovary, in which dynamic reconstruction and degeneration occur during folliculogenesis, atresia, ovulation, corpus luteum formation and luteolysis. In growing follicles, tenascin was only detected in the theca externa layer, while fibronectin was detected in the theca externa layer, theca interna layer and basement membrane. During follicular atresia, granulosa cells, which are surrounded by the basement membrane, began to die through apoptosis. In atretic follicles, tenascin was detected in the basement membrane and theca externa layer. Distribution of fibronectin in atretic follicles was similar to that in healthy growing follicles, except that granulosa cells were slightly immunopositive for fibronectin. In young corpus luteum, luteal cells exhibit high 3 beta-hydroxysteroid dehydrogenase (3-HSD) activity, an enzyme indispensable for progesterone production. Tenascin was barely detected in young luteal cells. 3 beta-HSD activity in luteal cells declines with corpus luteum age, and in older corpus luteum there is an increase in apoptotic death of luteal cells. Tenascin was intensely immunopositive in old luteal cells. In contrast, fibronectin immunostaining in luteal cells was relatively constant during corpus luteum formation and luteolysis. Our observations suggest that tenascin is critical in controlling the degenerative changes of tissues in mouse ovaries. Moreover, in all circumstances observed in this study, tenascin always co-localized with fibronectin, suggesting fibronectin is indispensable for the function of tenascin., ZOOLOGICAL SOC JAPAN, Feb. 2005, ZOOLOGICAL SCIENCE, 22 (2), 237 - 245, web_of_science

    Scientific journal

  • Vitellogenin-immunohistochemistry in the liver and the testis of the Medaka, Oryzias latipes, exposed to 17 beta-estradiol and p-nonylphenol

    K Kobayashi; S Tamotsu; K Yasuda; T Oishi

    Vitellogenin (VTG) produced in male fish has been used for a biomarker to study endocrine disrupters. However, the characteristics of VTG produced in male fish have not been studied well. In this study, we investigated the localization of VTG in the liver and the testis of male medaka (Oryzias latipes) treated with 17 beta-estradiol (E2) and p-nonylphenol (NP). The male fish were exposed to 1 mu g/L E2 and 500 mu g/L NIP for 1-12 days. Control groups were kept in water including only vehicle. The frozen sections of the liver and the testis were stained with immunohistochemical methods using an antiserurn against medaka VTG as the first antibody. In the E2 and NP treated liver, the hepatocytes showed immunoreactivity. In particular, the cytoplasm close to the cell membrane surrounding the sinusoids was strongly immunopositive. In the testis of both treatments, the interstitial tissues and the cells (spermatocytes) in the seminiferous tubules were immunopositive. The concentration of VTG became gradually higher in both tissues with longer treatments. These results suggest that germ cells in the testis treated with E2 and NP are able to incorporate and accumulate VTG., ZOOLOGICAL SOC JAPAN, Apr. 2005, ZOOLOGICAL SCIENCE, 22 (4), 453 - 461, web_of_science

    Scientific journal

  • Effects of estrogen (17β-estradiol) and p-\n nonylphenol on the development of immune organs in male Japanese quail.

    YASUDA Keiko; Razia S他

    ニホンウズラに17β-estradilolとp-nonyphenolを投与し、免疫器官に対する影響について検討した。17β-estradilolと\n p-nonyphenolによってウズラの脾臓、胸腺においてリンパ球の現象や結合組織の増加等の変化が起こり、免疫機能に\n 影響することが示唆された。, 2005, Environmental Sciences, 12, 99-110

  • Identification of a new theca/interstitial cell-specific gene and its biological role in growth of mouse ovarian follicles at the gonadotropin-independent stage.

    Aoyama M; Shiraishi A; Matubara S; Horie K; Osugi T; Kawada T; Yasuda K; Satake H

    Theca/interstitial cells are responsible for the growth and maturation of ovarian follicles. However, little is known about the theca/interstitial cell-specific genes and their functions. In this study, we explored transcriptomes of theca/interstitial cells by RNA-seq, and the novel biological roles of a theca cell marker, asporin (Aspn)/periodontal ligament-associated protein 1 (PLAP-1). RNA-seq detected 432 and 62 genes expressed specifically in theca/interstitial cells and granulosa cells isolated from 3-weeks old mouse ovaries. Gene ontology analysis demonstrated that these genes were largely categorized into four major groups: extracellular matrix organization-related terms, chemotaxis-related terms, the angiogenesis-related terms, and morphogenesis-related terms. In situ hybridization demonstrated that the newly detected representative gene, Aspn/PLAP-1, was detected specifically in the outer layer of theca cells in contrast with the expression of the basal lamina-specific gene, Nidgen-1. Intriguingly, an Aspn/PLAP-1 antibody completely arrested the growth of secondary follicles that is the gonadotropin-independent follicle developmental stage. Furthermore, transforming growth factor-β (TGF-β)-triggered signaling was induced by the Aspn/PLAP-1 antibody treatment, which is consistent with the inhibitory effect of Aspn/PLAP-1 on TGF-β. Altogether, these results suggest that theca cells are classified into subpopulations on the basis of new marker genes and their biological functions, and provide evidence that Aspn/PLAP-1 is expressed exclusively in the outer layer of theca cells and plays a pivotal role in the growth of secondary follicles via downregulation of the canonical TGF-β signaling cascade., Aug. 2019, Frontiers in Endocrinology, 10, 553 - 553, True, doi;pubmed;pmc

    Scientific journal

  • In situ observation of cellular organelles with a contact x-ray microscope

    M. Kado; M. Kishimoto; S. Tamotsu; K. Yasuda; K. Shinohara

    A contact x-ray microscope coupled with a highly intense laser plasma soft x-ray source has been developed and in situ observations of cellular organelles have been conducted. The soft x-rays were generated by irradiating a high power laser pulse onto a thin-foiled gold target and about 1.3x10(15) photons/sr were obtained, which allowed the inner structures of live wet biological cells to be imaged. Single shot flash imaging is a key technique to image cellular organelles of live biological cells avoiding degradation of the spatial resolution of the images resulting from motion blur and radiation damage. The use of a fluorescence microscope to identify cellular organelles in conjunction with the soft x-ray microscope has allowed several cellular organelles to be identified precisely in the soft x-ray images. Combining the fluorescence microscope with the soft x-ray microscope will be very useful and will establish the technique as a powerful tool to analyze living function of biological cells., IOP PUBLISHING LTD, 2013, 11TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY (XRM2012), 463, doi;web_of_science

    International conference proceedings

  • Observation of Organelles in Leydig Cells by Contact Soft X-Ray Microscopy with a Laser Plasma X-Ray Source

    M. Kado; M. Ishino; S. Tamotsu; K. Yasuda; M. Kishimoto; M. Nishikino; Y. Kinjo; K. Shinohara

    We observed the same biological specimens for comparison of the images by contact soft x-ray microscopy with a laser plasma x-ray source with those by confocal laser microscopy. Images of wet Leydig cells were directly comparable for organelles and showed that actin filaments and mitochondria were clearly identified in the soft x-ray images., AMER INST PHYSICS, 2011, 10TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY, 1365, 391 - 394, doi;web_of_science

    International conference proceedings

  • The localization and role of Thy-1 during folliculogenesis in mouse ovary

    Saori Itami; Keiko Yasuda; Satoshi Tamotsu

    ZOOLOGICAL SOC JAPAN, Dec. 2006, ZOOLOGICAL SCIENCE, 23 (12), 1217 - 1217, web_of_science

  • Chenges in the distribution of Tenascin and Fibronectin in murine ovary

    Keiko Yasuda; Emi Hagiwara; Akiko Takeuchi; Chinatsu Mukai; Chiyuki Matsui; Atsushi Sakai; Satoshi Tamotsu

    ZOOLOGICAL SOC JAPAN, Dec. 2004, ZOOLOGICAL SCIENCE, 21 (12), 1336 - 1336, web_of_science

  • Effects of 17β-Estradiol and p-Nonylphenol on the Gonads and the Papillary Process Formation in Vivo and in Vitro.

    YASUDA Keiko

    2001, Environmental Science, 8, 211

  • Relationship between the number of annuli of adult antenna and the length of embryonic and larval period in Samia cynthia ricini

    K Yasuda; S Takahashi

    In the eri-silkworm, Samia cynthia ricini, the adult antennal flagellum is segmented into many annuli. Although the number of annuli is an important parameter in the morphogenesis of adult antenna, it is not clear when and how the number of annuli is determined. In the present study the fifth instar larva of the eri-silkworm was studied histologically to clarify when the antennal imaginal disk began morphogenesis and when the number of annuli of adult antennal flagellum was determined. In addition we studied whether the length of the embryonic and larval period might have any influence on the number of annuli in the eri-silkworm, and the influence of other factors such as body size and sex was also examined. Serial histological study of the imaginal disk during the larval period suggested that the number of annuli was determined by the second day after gut-purging, since the segmentation of the pupal antenna was almost finished by this time and the number of segments of pupal antenna was nearly equal to the number of annuli of adult antenna. The embryonic and larval period was closely related with the number of annuli. When the insects were reared at 25 degrees C, the number of annuli was almost equal to the number of the days in which the insects passed from oviposition to gut-purging. In addition, the number of annuli tended to increase one by one from 27 to 34 as the embryonic and larval period was extended day by day from 28 to 35 days. When the insects were reared at 18 degrees C, the larval period was doubled, whereas the number of annuli remained in the same range (28-34) as that reared at 25 degrees C. The body size did not correlate with the number of annuli. Although the number of annuli was significantly larger in female than in male, this difference seemed to be due to the difference in the length of the embryonic and larval period in both sexes., ZOOLOGICAL SOC JAPAN, Jun. 1997, ZOOLOGICAL SCIENCE, 14 (3), 435 - 442, web_of_science

    Scientific journal

  • CYTOKINES STIMULATE DIPEPTIDYL PEPTIDASE-IV EXPRESSION ON HUMAN LUTEINIZING GRANULOSA-CELLS

    H FUJIWARA; M FUKUOKA; K YASUDA; M UEDA; K IMAI; Y GOTO; H SUGINAMI; H KANZAKI; M MAEDA; T MORI

    We have previously reported that dipeptidyl peptidase-IV (DPPIV) is a differentiation antigen for human granulosa cells that is initially expressed during corpus luteum formation. To investigate the involvement of cytokines in luteal cell differentiation, we examined the expression and activity of DPPIV in human luteinizing granulosa cells cultured in vitro. Human granulosa cells obtained from patients who had undergone in vitro fertilization were cultured for 7 days in the absence (controls) or presence of hCG (1 U/mL), tumor necrosis factor-alpha (TNF alpha; 10 ng/ml), or interIeukin 1-alpha (IL-1 alpha; 10 ng/mL). Flow cytometry showed that the percentage of cultured granulosa cells treated with TNF alpha and IL-1 alpha that was positive for DPPIV expression was significantly higher than that in controls (43.7 +/- 5.4% and 43.4 +/- 5.6%, respectively, vs. 21.7 +/- 3.5%; P < 0.01), whereas hCG treatment produced no remarkable difference in DPPIV expression (24.0 +/- 5.2%). The DPPIV activity of cells treated with TNF alpha and IL-1 alpha was also significantly higher than that of controls, whereas hCG treatment produced no significant difference from control values. These findings indicate that TNF alpha and IL-1 alpha stimulate DPPIV expression and activity in human luteinizing granulosa cells in vitro and suggest the involvement of cytokines in the differentiation of granulosa cells during corpus luteum formation., ENDOCRINE SOC, Oct. 1994, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 79 (4), 1007 - 1011, web_of_science

    Scientific journal

  • A NEW MONOCLONAL-ANTIBODY (POG-1) DETECTS A DIFFERENTIATION ANTIGEN OF PORCINE GRANULOSA AND THECAL CELLS AND INDICATES HETEROGENEITY OF THECAL-STROMAL CELLS

    H FUJIWARA; M UEDA; M FUKUOKA; K YASUDA; K IMAI; K TAKAKURA; H KANZAKI; H SUGINAMI; T MORI; M MAEDA

    To identify the differentiation antigen of ovarian cells, we raised a murine monoclonal antibody (POG-1 antibody) reactive to porcine granulosa and thecal cells in the ovary. Immunofluorescence staining showed that expression of the POG-1 antigen on granulosa and theca interns cells increased gradually in accordance with follicular development. The thecal cells just outside the basal lamina surrounding the follicles did not express the antigen, whereas some stromal cells around the theca externa layer in the large follicles did express it. These expression profiles indicated the heterogeneity of thecal-stromal cells and that the POG-1 antigen was a differentiation-related antigen of granulosa and thecal cells. Luteal cells also expressed the antigen. In organs other than the ovary, some endocrine and exocrine cells, such as Leydig cells and secretory cells of the breast, expressed the antigen. The POG-1 antigen was purified from granulosa cells by immunoaffinity chromatography. Polyacrylamide gel electrophoresis profiles showed that the antigen consisted of two specific proteins; the major one had a molecular mass of 77, and the other had a molecular mass of 81 kilodaltons. Analysis of the purified POG-1 antigen may contribute to understanding the differentiation mechanism of granulosa and thecal cells., ENDOCRINE SOC, Mar. 1994, ENDOCRINOLOGY, 134 (3), 1132 - 1138, web_of_science

    Scientific journal

  • HUMAN-LEUKOCYTE ANTIGEN-DR IS A DIFFERENTIATION ANTIGEN FOR HUMAN GRANULOSA-CELLS

    H FUJIWARA; M UEDA; K IMAI; M FUKUOKA; K YASUDA; K TAKAKURA; H SUGINAMI; H KANZAKI; H INOKO; T MORI; M MAEDA

    We raised a murine monoclonal antibody, OG-3, which reacts with human granulosa cells. Immunohistologically, OG-3 antigen was weakly expressed on the granulosa cells of some growing and atretic follicles, but not on those of preovulatory follicles. After ovulation, the antigen expression rapidly increased on granulosa cells during corpus luteum formation. The antigen expression on granulosa/large luteal cells decreased in the mid-luteal phase, but increased again in the late luteal phase. In early pregnancy, OG-3 antigen expression on large luteal cells increased after 7 wk of gestation. The OG-3 antigen distribution in various organs resembled that of human leukocyte antigen (HLA) class II molecules. An HLA-class II-positive human B cell line (AKIBA) and a murine L-cell transfectant expressing HLA-DR antigen were positive for OG-3 antigen, whereas an HLA-class II-negative human T-cell line and L-cell transfectants expressing HLA-DP and DQ antigens were negative. The molecular mass of OG-3 antigen purified from AKIBA cells was 32-35 kDa. The staining profiles in ovaries with anti-HLA-DR or anti-HLA-class II antibodies were similar to that with OG-3. These results indicate that OG-3 antigen is identical to HLA-DR, and that HLA-DR is a differentiation antigen for human granulosa cells., SOC STUDY REPRODUCTION, Oct. 1993, BIOLOGY OF REPRODUCTION, 49 (4), 705 - 715, web_of_science

    Scientific journal

  • A DIFFERENTIATION-RELATED MOLECULE ON THE CELL-SURFACE OF HUMAN GRANULOSA-CELLS

    H FUJIWARA; M MAEDA; M UEDA; M FUKUOKA; K YASUDA; K IMAI; K TAKAKURA; T MORI

    We raised a monoclonal antibody (OG-1) specifically reactive to human ovarian granulosa cells. Indirect immunofluorescence staining of freshly isolated granulosa cells from preovulatory follicles showed the OG-1 antigen on the cell surface of granulosa cells. The antigen was purified from granulosa cells by immunoaffinity chromatography. Under reducing or nonreducing conditions, the sodium dodecyl sulfatepolyacrylamide gel electrophoresis profile of the purified antigen showed two specific protein bands, corresponding to mol wt of 125 and 145 kilodaltons. Immunohistologically, the OG-1 antigen on granulosa cells of primary follicles seemed to increase during follicular development. After ovulation, the antigen was detected on granulosa cells during corpus luteum formation. The antigen expression began to decrease on corpus luteum days 10-12 and disappeared on corpus luteum days 13-14. Luteal cells of pregnancy did not express the OG-1 antigen. Granulosa cells in atretic follicles expressed the OG-1 antigen. On the contrary, thecal or small luteal cells did not express it throughout the menstrual cycle. These results indicate that OG-1 antigen is a differentiation-related surface antigen of human granulosa cells., ENDOCRINE SOC, Apr. 1993, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 76 (4), 956 - 961, web_of_science

    Scientific journal

  • EFFECTS OF INTERFERON ON THE STEROIDOGENIC FUNCTIONS AND PROLIFERATION OF IMMATURE PORCINE GRANULOSA-CELLS IN CULTURE

    K YASUDA; M FUKUOKA; H FUJIWARA; T MORI

    Recent studies suggest the relevance of several cytokines to the growth and differentiation of granulosa cells. In the present study, we investigated the effects of interferon (IFN) on the steroidogenic functions and proliferation of immature porcine granulosa cells. Human IFN-alpha inhibited FSH-induced progesterone secretion in a concentration-dependent manner. The effect of IFN-alpha was significant at a concentration as low as 10 pg/ml. Maximal inhibitory concentrations (10-50 ng/ml) of IFN-alpha reduced FSH-induced progesterone secretion by 70%. In contrast, estradiol secretion induced by FSH was significantly enhanced by relatively high concentrations (1-50 ng/ml) of IFN-alpha. IFN-alpha (0.1-10 ng/ml) reduced cAMP generation in response to FSH by as much as 80%, although its effect was not concentration-dependent. The proliferation of cultured granulosa cells was inhibited by IFN-alpha in a concentration-dependent manner. Human IFN-gamma did not affect granulosa cell functions. The stimulation of estradiol secretion and the inhibition of cell proliferation induced by IFN-alpha in cultured porcine granulosa cells in this study are in contrast with the effects of IL-1, which, as we reported previously, inhibited both progesterone and estradiol secretion and stimulated cell growth in these cell cultures. Such differences in the mode of action of cytokines may contribute to the regulation of granulosa cell functions under physiological or pathological conditions., SOC STUDY REPRODUCTION, Dec. 1992, BIOLOGY OF REPRODUCTION, 47 (6), 931 - 936, web_of_science

    Scientific journal

  • HUMAN LUTEAL CELLS EXPRESS DIPEPTIDYL PEPTIDASE-IV ON THE CELL-SURFACE

    H FUJIWARA; M MAEDA; K IMAI; M FUKUOKA; K YASUDA; K TAKAKURA; T MORI

    We previously reported that human theca interna cells and small luteal cells express membrane-bound aminopeptidase N, and suggested that membrane-bound peptidases are involved in folliculogenesis and luteal function by regulating extracellular peptide concentrations. In this study, we examined the expression of dipeptidyl peptidase IV (DPP IV), which is a membrane-bound peptidase and has its catalytic domain at extracellular sites, in human granulosa cells, thecal cells of growing, preovulatory, and atretic follicles, as well as corpora lutea. Indirect immunofluorescence staining of ovarian tissues with specific monoclonal antibodies revealed that DPP IV was present in large and small luteal cells in corpora lutea. DPP IV peptidase activity was also detected histochemically in corpora lutea. In growing, preovulatory, and atretic follicles, there was weak immunoreactivity and DPP IV peptidase activity on luteinized theca interna cells, but not on granulosa cells. The expression of DPP IV on the cell surface of large and small luteal cells was confirmed by indirect immunofluorescence staining of freshly isolated luteal cells. These results indicate that DPP IV is a useful surface differentiation marker of human luteal cells and suggest that peptidases are involved in luteal function., ENDOCRINE SOC, Nov. 1992, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 75 (5), 1352 - 1357, web_of_science

    Scientific journal

  • INTERACTIONS BETWEEN INTERFERON-GAMMA, TUMOR-NECROSIS-FACTOR-ALPHA, AND INTERLEUKIN-1 IN MODULATING PROGESTERONE AND ESTRADIOL PRODUCTION BY HUMAN LUTEINIZED GRANULOSA-CELLS IN CULTURE

    M FUKUOKA; K YASUDA; H FUJIWARA; H KANZAKI; T MORI

    We have reported that the cytokines, interleukin-1 (IL-1), tumour necrosis factor alpha (TNFalpha), and interferon (IFN)alpha, beta, and gamma modulate the steroidogenic function of human luteinized granulosa cells in culture. In the present study we examined the interactions between these cytokines in modulating progesterone and oestradiol production by these cells. Neither IL-1 nor TNFalpha had significant effects on human chorionic gonadotrophin (HCG)-stimulated progesterone production, whereas IFNgamma (1 - 10 ng/ml) significantly reduced HCG-stimulated progesterone production by 26-37%. Concomitant treatment with IL-1 (I ng/ml) did not further enhance the inhibitory effect of IFNgamma on HCG-stimulated progesterone production. In contrast, the combination of TNFalpha (1 ng/ml) and IFNgamma (10 ng/ml) acted synergistically to markedly inhibit HCG-stimulated progesterone production by 81%. In addition, IL-1 and TNFalpha, neither of which was effective alone, acted synergistically to reduce significantly HCG-stimulated progesterone production by 30%. The combination of TNFalpha and IFNgamma also markedly inhibited follicle stimulating hormone (FSH)-stimulated oestradiol production by 97%, a significantly greater inhibition than that obtained with either cytokine alone. These results suggest that the cytokines may interact to modulate the steroidogenic function of luteal cells in the developing corpus luteum., OXFORD UNIV PRESS UNITED KINGDOM, Nov. 1992, HUMAN REPRODUCTION, 7 (10), 1361 - 1364, web_of_science

    Scientific journal

  • CYTOKINE MODULATION OF PROGESTERONE AND ESTRADIOL SECRETION IN CULTURES OF LUTEINIZED HUMAN GRANULOSA-CELLS

    M FUKUOKA; K YASUDA; N EMI; H FUJIWARA; M IWAI; K TAKAKURA; H KANZAKI; T MORI

    To clarify the possible roles of cytokines in the regulation of luteal cell function, we examined the effects of interferon (IFN), interleukin-1 (IL-1), and tumor necrosis factor (TNF) on progesterone and estradiol secretion in cultures of luteinized human granulosa cells. IFN-gamma reduced hCG-stimulated progesterone secretion in a concentration-dependent manner; at its maximal inhibitory concentration (10 ng/mL), IFN-gamma reduced progesterone secretion to 20% of that in the hCG-stimulated controls. Whereas other IFN (alpha and beta) reproduced the inhibitory effect of IFN-gamma, IL-1 and TNF had no effect on hCG-stimulated progesterone secretion at concentrations of 1 and 10 ng/mL. IFN-gamma also markedly reduced FSH-stimulated estradiol secretion. Unlike their effects on hCG-stimulated progesterone secretion, IL-1 and TNF reproduced the inhibitory effect of IFN-gamma on FSH-stimulated estradiol secretion. IFN-gamma significantly reduced both hCG- and FSH-stimulated cAMP generation in granulosa cells. IL-1 and TNF inhibited FSH-stimulated cAMP generation, but they did not inhibit hCG-stimulated cAMP generation. None of these cytokines reduced forskolin-stimulated cAMP generation, thus suggesting that these cytokines affect steps proximal to cAMP generation without affecting cAMP generation itself. IFN-gamma also reduced progesterone secretion in response to (Bu)2cAMP, suggesting that it also affects steps distal to cAMP generation. This study has demonstrated that cytokines modulate the steroidogenesis of luteinized human granulosa cells in vitro; the results suggest that cytokines may play permissive roles in regulating luteal cell function., ENDOCRINE SOC, Jul. 1992, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 75 (1), 254 - 258, web_of_science

    Scientific journal

  • SYNERGISTIC ACTIONS OF CYTOKINES AND GROWTH-FACTORS IN ENHANCING PORCINE GRANULOSA-CELL GROWTH

    M FUKUOKA; K YASUDA; S TAII; T MORI

    In our studies of the growth-promoting effect of a cytokine, interleukin-1 (IL-1), on cultured porcine granulosa cells, we found that the potency of IL-1 action correlated with the serum concentration in the culture medium and that IL-1 acted synergistically with insulin to increase the number of cells in the presence of low serum concentrations (0.1-1%). With granulosa cells maintained in a quiescent state under serum-free conditions, we therefore examined the effects of combined treatment with IL-1 and peptide growth factors, including insulin, on [H-3]thymidine incorporation by these cells. IL-1 by itself enhanced [H-3]thymidine incorporation in a concentration-dependent manner. Moreover, IL-1 acted synergistically with insulin, epidermal growth factor (EGF), or fibroblast growth factor (FGF) to enhance [H-3]thymidine incorporation. Combinations of maximally effective concentrations of insulin (1-mu-g/ml), EGF (1 ng/ml), or FGF (50 ng/ml) with the maximally effective concentration of IL-1 (10 ng/ml) increased the levels of [H-3]thymidine incorporation to 10-, 22-, and 20-fold, respectively, over the control values. Whereas IL-2 (0.1-100 ng/ml) did not affect [H-3]thymidine incorporation, tumor necrosis factor alpha (TNF-alpha) stimulated [H-3]thymidine incorporation by itself and reproduced the actions of IL-1 to act synergistically with insulin, EGF, or FGF. When IL-1 and TNF-alpha were added together in relatively low concentrations (1 ng/ml each), the combination had synergistic effects in enhancing [H-3]thymidine incorporation. The present study demonstrates that cytokines and peptide growth factors act synergistically to markedly enhance porcine granulosa cell growth in vitro., JAPAN ENDOCRINE SOCIETY, Jun. 1992, ENDOCRINOLOGIA JAPONICA, 39 (3), 277 - 288, web_of_science

    Scientific journal

  • DIFFERENTIAL EXPRESSION OF AMINOPEPTIDASE-N ON HUMAN OVARIAN GRANULOSA AND THECA CELLS

    H FUJIWARA; M MAEDA; K IMAI; M FUKUOKA; K YASUDA; K HORIE; K TAKAKURA; S TAII; T MORI

    The expression of aminopetidase-N and neutral endopeptidase in human ovarian tissue was examined using specific monoclonal antibodies for each of these peptidases and histochemical staining for enzyme activity. Aminopeptidase-N is a membrane-bound metalloprotease catalyzing the removal of N-terminal amino acids from peptides and was detected by immunofluorescence staining on theca interna cells in secondary follicles and on luteinized thecal cells in preovulatory follicles and corpora lutea. However, aminopeptidase-N was not detected on granulosa cells. Peptidase activity was also detected by histochemical staining on theca interna cells and luteinized thecal cells. Luteinized granulosa cells showed peptidase activity, despite the lack of aminopeptidase-N. Neutral endopeptidase was not detected in ovarian granulosa and thecal cells. These observations indicate that aminopeptidase-N can be a useful surface marker for thecal cells., ENDOCRINE SOC, Jan. 1992, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 74 (1), 91 - 95, web_of_science

    Scientific journal

  • LUTEINIZING-HORMONE INDUCES PROGESTERONE-RECEPTOR GENE-EXPRESSION IN CULTURED PORCINE GRANULOSA-CELLS

    M IWAI; K YASUDA; M FUKUOKA; T IWAI; K TAKAKURA; S TAII; S NAKANISHI; T MORI

    We have examined the effect of LH on the regulation of the progesterone receptor (PR) in cultured porcine granulosa cells. In this study we used the RNase protection assay to evaluate the PR mRNA levels with a porcine cDNA clone isolated by the polymerase chain reaction (PCR) method. This clone was regarded as part of the porcine PR cDNA because of its 98.3% and 95.7% homology to the hormone-binding domain of human PR cDNA in amino acid and nucleotide sequences, respectively. Treatment with LH (500 ng/ml) increased porcine PR mRNA to a maximum level of 8.6 +/- 1.1-fold (mean +/- SE) after 3-h exposure. This induction was mimicked by (Bu)2cAMP as well as by FSH and hCG, and the increased PR caused by LH and (Bu)2cAMP occurred in a dose-dependent manner. Basal and LH-induced PR mRNA levels were not affected by progesterone (100 ng/ml), estrogen (100 ng/ml), and RU 486 (10 ng/ml) at 3 h. The mechanism of the increased PR mRNA levels was studied in the presence of actinomycin-D and cycloheximide. While inhibition of RNA synthesis with actinomycin-D blocked LH-induced PR mRNA expression, inhibition of protein synthesis with cycloheximide increased basal and LH-induced PR mRNA levels. These results indicate that the expression of PR mRNA is positively regulated by LH, and this induction does not require ongoing protein synthesis. There may be a cycloheximide-sensitive mechanism that modulates PR mRNA stability. From our results were suspect that progesterone modulates ovarian function through LH-induced PR in granulosa cells., ENDOCRINE SOC, Sep. 1991, ENDOCRINOLOGY, 129 (3), 1621 - 1627, web_of_science

    Scientific journal

  • INHIBITORY EFFECTS OF INTERLEUKIN-1 ON FOLLICLE-STIMULATING-HORMONE INDUCTION OF AROMATASE-ACTIVITY, PROGESTERONE SECRETION, AND FUNCTIONAL LUTEINIZING-HORMONE RECEPTORS IN CULTURES OF PORCINE GRANULOSA-CELLS

    K YASUDA; M FUKUOKA; S TAII; K TAKAKURA; T MORI

    SOC STUDY REPRODUCTION, Dec. 1990, BIOLOGY OF REPRODUCTION, 43 (6), 905 - 912, web_of_science

    Scientific journal

  • INTERLEUKIN-2 RECEPTOR P55(TAC) INDUCING ACTIVITY IN PORCINE FOLLICULAR FLUIDS

    K TAKAKURA; S TAII; M FUKUOKA; K YASUDA; Y TAGAYA; J YODOI; T MORI

    ENDOCRINE SOC, Aug. 1989, ENDOCRINOLOGY, 125 (2), 618 - 623, web_of_science

    Scientific journal

  • INHIBITORY EFFECTS OF INTERLEUKIN-1 ON LUTEINIZING HORMONE-STIMULATED ADENOSINE-3',5'-MONOPHOSPHATE ACCUMULATION BY CULTURED PORCINE GRANULOSA-CELLS

    M FUKUOKA; S TAII; K YASUDA; K TAKAKURA; T MORI

    ENDOCRINE SOC, Jul. 1989, ENDOCRINOLOGY, 125 (1), 136 - 143, web_of_science

    Scientific journal

  • INTERLEUKIN-1 STIMULATES GROWTH AND INHIBITS PROGESTERONE SECRETION IN CULTURES OF PORCINE GRANULOSA-CELLS

    M FUKUOKA; K YASUDA; S TAII; K TAKAKURA; T MORI

    ENDOCRINE SOC, Feb. 1989, ENDOCRINOLOGY, 124 (2), 884 - 890, web_of_science

    Scientific journal

  • Modulation of porcine granulosa cell functions by interleukin-1

    Takahide Mori; Masatsune Fukuoka; Keiko Yasuda; Kenji Takakura; Toshiko Iwai; Shunzo Taii

    Increasing evidence suggests that functions of the immune system and gonads are closely related with each other. In cultures of granulosa and luteal cells, macrophages have been shown to modulate steroidogenic functions. In this paper we present the modulatory effects of interleukin-1, a cytokine produced predominantly by activated macrophages, on gonadotropin-induced differentiation, as well as growth of cultured porcine granulosa cells. © 1989., 1989, Steroids, 54 (5), 543 - 552, doi;pubmed

    Scientific journal

  • INTERLEUKIN-1 INHIBITS LUTEINIZATION OF PORCINE GRANULOSA-CELLS IN CULTURE

    M FUKUOKA; T MORI; S TAII; K YASUDA

    ENDOCRINE SOC, Jan. 1988, ENDOCRINOLOGY, 122 (1), 367 - 369, web_of_science

  • Observation of organelle by a laser plasma x-ray microscope

    Masataka Kado; Maki Kishimoto; Masahiko Ishino; Satoshi Tamotsu; Keiko Yasuda; Kunio Shinohara

    Contact x-ray microscopy has a potential to image wet biological specimens in natural condition. It is very important to identify obtained features in the x-ray images, since x-ray microscopes have potential to image features that have not been visualized yet. We have proposed to compare the x-ray images of the biological specimens with the fluorescence images and to identify the features found in the x-ray images. We have succeeded to observe fine structures of the cellular organelles such as mitochondria by the soft x-ray microscope., AMER INST PHYSICS, 2012, LASER-DRIVEN RELATIVISTIC PLASMAS APPLIED TO SCIENCE, ENERGY, INDUSTRY, AND MEDICINE, 1465, 246 - 250, doi;web_of_science

    International conference proceedings

  • Flash imaging of fine structures of cellular organelles by contact x-ray microscopy with a high intensity laser plasma x-ray source

    Masataka Kado; Masahiko Ishino; Maki Kishimoto; Satoshi Tamotsu; Keiko Yasuda; Yasuhito Kinjo; Kunio Shinohara

    X-ray flash imaging by contact microscopy with a highly intense laser-plasma x-ray source was achieved for the observation of wet biological cells. The exposure time to obtain a single x-ray image was about 600 ps as determined by the pulse duration of the driving laser pulse. The x-ray flash imaging makes it possible to capture an x-ray image of living biological cells without any artificial treatment such as staining, fixation, freezing, and so on. The biological cells were cultivated directly on the surface of the silicon nitride membranes, which are used for the x-ray microscope. Before exposing the cells to x-rays they were observed by a conventional fluorescent microscope as reference, since the fluorescent microscopes can visualize specific organelles stained with fluorescent dye. Comparing the x-ray images with the fluorescent images of the exact same cells, each cellular organelle observed in the x-ray images was identified one by one and actin filaments and mitochondria were clearly identified in the x-ray images., SPIE-INT SOC OPTICAL ENGINEERING, 2011, ADVANCES IN X-RAY/EUV OPTICS AND COMPONENTS VI, 8139, doi;web_of_science

    International conference proceedings

  • Observations of the intense soft x-ray emissions from ultra thin Au films irradiated with high contrast laser pulses

    Masahiko Ishino; Masataka Kado; Masaharu Nishikino; Kunio Shinohara; Satoshi Tamotsu; Keiko Yasuda; Noboru Hasegawa; Maki Kishimoto; Toshiyuki Ohba; Tetsuya Kawachi

    Observation of soft x-ray emissions from laser produced plasmas using ultra thin film targets has been carried out. Au ultra thin films deposited on silicon nitride membranes were irradiated with a high contrast Nd:glass laser pulses. The spectral properties of emitted soft x-rays were monitored with an x-ray spectrograph from the membrane side. The observed emission intensities had a clear dependence on the Au film thickness. The results suggest that most of the laser energy irradiated is absorbed by the Au films and few of the energy goes into the silicon nitride membranes, which means an efficient laser energy deposition to the ultra thin Au film target and a high energy conversion rate from laser to x-rays., SPIE-INT SOC OPTICAL ENGINEERING, 2010, FRONTIERS IN ULTRAFAST OPTICS: BIOMEDICAL, SCIENTIFIC, AND INDUSTRIAL APPLICATIONS X, 7589, doi;web_of_science

    International conference proceedings

MISC

  • Chemical Shift Images of Organelles in Leydig cells of Mice Testes

    T. Ejima; Y. Neichi; M. Yanagihara; M. Kado; M. Ishino; K. Yasuda; S. Tamotsu

    Soft X-ray transmission images of Leydig cells of mice testes changing incident wavelength were observed with the use of a contact microscope. After normalization of transmission images, absorbance images were obtained and compared with a visible differential interference image. Some organelles were identified by the image comparison, and absorption spectra of the organelles were obtained from the absorbance images. The absorption spectra show that peak structures are different depending on the observed organelles. The structures and the positions of organelles were clearly identified at C-K absorption., IOP PUBLISHING LTD, 2013, 11TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY (XRM2012), 463, doi;web_of_science

  • Imaging of fine structures of cellular organelles in hydrated biological cells by a soft x-ray microscope combined with a fluorescence microscope

    Masataka Kado; Maki Kishimoto; Satoshi Tamotsu; Keiko Yasuda; Masato Aoyama; Kunio Shinohara

    We have proposed and developed a new hybrid microscopy system using a soft x-ray microscope and a fluorescence microscope imaging the same biological cells at the nearly same time. Combining the powerful advantages such as high spatial resolution of the soft x-ray microscope and the accurate organelle identification of the fluorescence microscope, we can observe fine structures of the cellular organelles in live hydrated biological cells in situ. Staining the cells with several fluorescent dyes such as Mito-tracker, Phalloidin, and DAPI, the soft x-ray images of the cells have been directly compared with the fluorescent images and the cellular organelles such as mitochondria, actin filaments, and chromosomes in the soft x-ray images have been clearly identified. Since the soft x-ray microscope has higher spatial resolution than that of the fluorescence microscope, not only shape of the cellular organelles but also the fine structures of the cellular organelles of the live biological cells have been clearly observed for the first time. (C) (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only., SPIE-INT SOC OPTICAL ENGINEERING, 2013, X-RAY LASERS AND COHERENT X-RAY SOURCES: DEVELOPMENT AND APPLICATIONS X, 8849, doi;web_of_science

  • 高輝度軟X線源を用いた軟X線顕微鏡による細胞内器官の高解像観察

    加道雅孝; 岸本牧; 保智己; 安田恵子; 青山雅人; 江島丈雄; 刀祢重信; 篠原邦夫; 篠原邦夫

    Mar. 2016, 大阪大学レーザーエネルギー学研究センター共同利用・共同研究成果報告書, 2015, 56 - 57, j_global;url

  • 高輝度軟X線源を用いた軟X線顕微鏡による細胞内器官の高解像観察

    加道雅孝; 岸本牧; 保智己; 安田恵子; 青山雅人; 江島丈雄; 篠原邦夫; 篠原邦夫

    Mar. 2015, 大阪大学レーザーエネルギー学研究センター共同利用・共同研究成果報告書, 2014, 70 - 71, j_global;url

Books etc

  • Effects of endocrine disrupting substance on the development of gonads and immune organs in birds and fishes.

    YASUDA Keiko; Razia S (, Range: 分担)

    2002

  • Ovarian cytokines in regulation of granulosa cell function. (共著)

    YASUDA Keiko

    Progress in Endocrinology : The proceedings of the Ninth International Congress of Endocrinology, Nice, 1993, 577頁

  • Differential function of Luteal cell types in human and porcine luteogenesis. (共著)

    YASUDA Keiko

    Local Regulation of Ovarian Function : The Proceedings of the Second Organon Round Table Conference, Lund, 1992, 11-15巻277頁

  • Granulosa cell immunotropism. (共著)

    YASUDA Keiko

    Recent Advances in Ovarian Function : Basic and Clinical Researches., 1992, 31頁

  • Growth factor modulation of gonadotropin action on porcine granulosa cells. (共著)

    YASUDA Keiko

    Advances in Assisted Reproductive Technologies, 1990, 93頁

  • Regulation of steroidogenic function during luteinization(共著)

    YASUDA Keiko

    Development of Preimplantion Embryos and Their Environment, 1989, 117頁

Presentations

  • マウス卵巣のマクロファージはFSH刺激によってインターフェロンα7を産生する。

    YASUDA Keiko

    第43回日本比較内分泌学会, Nov. 2018, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台

  • マウス卵巣の卵胞形成過程で起こる莢膜細胞層形成機構の解明-卵胞は間質細胞を誘引し、増殖を促進する-

    YASUDA Keiko

    第43回日本比較内分泌学会大会, Nov. 2018, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台

  • 腫瘍壊死因子(TNFα)はマウス卵巣の卵胞閉鎖を誘導するのか?

    YASUDA Keiko

    第43回日本比較内分泌学会大会, Nov. 2018, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台

  • 莢膜/間質細胞特異的遺伝子の同定とゴナドトロピン非依存段階の卵巣内卵胞の成長における生物学的役割

    YASUDA Keiko

    第43回日本比較内分泌学会大会, Nov. 2018, 日本比較内分泌学会, 東北大学青葉山コモンズ 仙台

  • 腫瘍壊死因子α(TNFα)はマウス卵巣の卵胞閉鎖を誘導するのか?

    YASUDA Keiko

    第89回日本動物学会大会, Sep. 2018, 日本動物学会, 札幌コンベンションセンター 札幌

  • 莢膜/間質細胞特異的遺伝子の同定とゴナドトロピン非依存段階の卵巣内卵胞の成長における生物学的役割

    YASUDA Keiko

    第89回日本動物学会大会, Sep. 2018, 日本動物学会, 札幌コンベンションセンター 札幌

  • マウス卵巣の卵胞発育過程で起こる莢膜細胞層形成機構の解明-卵胞は間質細胞を誘引し、増殖を促進する-

    YASUDA Keiko

    第89回日本動物学会大会, Sep. 2018, 日本動物学会, 札幌コンベンションセンター 札幌

  • 抱卵するコケゴロモガキOstrea circumpictaの性システム

    YASUDA Keiko

    第65回日本生態学会大会, Mar. 2018, 日本生態学会, 札幌コンベンションセンター 札幌

  • 性周期におけるマウス卵巣内monocyte chemotactic protein-1(MCP-1)の局在と変動

    YASUDA Keiko

    第42回日本比較内分泌学会, Nov. 2017

  • エストロゲンによって促進されるマウス精巣血管新生におけるProkineticin2の関与

    YASUDA Keiko

    第42回日本比較内分泌学会, Nov. 2017

  • マウス卵巣の卵胞発育/卵胞閉鎖におけるトランスフォーミング増殖因子(TGF-β)の機能

    YASUDA Keiko

    第42回日本比較内分泌学会, Nov. 2017

  • ケモカインCCL21のマウス卵巣における局在解析と卵胞発育に対する機能

    YASUDA Keiko

    第42回日本内分泌学会, Nov. 2017

  • マウス卵巣の卵胞発育/卵胞閉鎖における腫瘍壊死因子α(TNFα)の機能

    YASUDA Keiko

    日本動物学会第88回大会, Sep. 2017

  • トランスクリプトーム解析によるマウス卵巣莢膜・間質細胞特異的発現遺伝子の同定

    YASUDA Keiko

    日本動物学会第88回大会, Sep. 2017

  • 性周期におけるマウス卵巣内マクロファージおよび末梢血単球のFSH受容体発現の変動

    YASUDA Keiko

    第41回二本比較内分泌学会大会及びシンポジウム, Dec. 2016

  • トランスクリプトーム解析によるマウス卵巣莢膜・間質細胞特異的発現遺伝子の同定

    YASUDA Keiko

    第41日本比較内分泌学会大会およびシンポジウム, Dec. 2016

  • マウス精巣血管新生におけるプロキネチシン2の役割

    YASUDA Keiko

    第41回日本比較内分泌学会大会及びシンポジウム, Dec. 2016

  • Identification of mouse ovarian interstitial cell-specific genes by transcriptome analysis.

    YASUDA Keiko; Aoyama M; Shiraishi A; Horiwe K; Osugi T; Yasuda K; Satake H

    The 22nd International Congress of Zoology and The 87 th meeting of Zoological Society of Japan, Nov. 2016

  • Changes of localizations and gene expressions of prokineticins and their receptors by gonadotrpin stimulation in mouse ovaries.

    YASUDA Keiko; Onishi A; Ono N; Aoyama M; Tamotsu S; Yasuda K

    CompBiol 2015 Hiroshima, Dec. 2015

  • The localizations of the transforming growth factor beta(TGF-beta) and uts receptors in mouse ovaries,.

    YASUDA Keiko; Fukasawa S; Aoyama M; Tamotsu S; Yasuda K

    CompBiol 2015 Hiroshima, Dec. 2015

  • The role of tumor necrosis factor (TNF)α on apoptosis of granulosa cells during follicle atresia in mouse ovaries.

    YASUDA Keiko; Hasumuro H; Uchida S; Shirokane M; Aoyama M; Tamotsu S; Yasuda K

    CompBiol 2015 Hiroshima, Dec. 2015

  • Are the adult Leydig cells migrating from mesonephros in the fetal mouse testes?

    YASUDA Keiko; Maeda E; Nisho S; Okazaki N; Aoyama M; Tamotsu S; Yasuda K

    CompBiol 2015 Hiroshima, Dec. 2015

  • Identification of mouse interstitial cell-specific genes by transcriptome analysis.

    YASUDA Keiko; Aoyama M; Shiraishi A; Horie K; Yasuda K; Satake H

    CompBiol 2015 Hiroshima, Dec. 2015

  • トランスクリプトーム解析によるマウス卵巣間質細胞特異的遺伝子の\n同定

    YASUDA Keiko

    日本動物学会第86回大会, Sep. 2015

  • Localization of Tumor Necrosis Factor Receptor 2(TNFR2) in mouse Ovaries.

    YASUDA Keiko; Hasmuro H; Uchida S; Shirokane M; Aoyamna M; Tamotsu S; Yasuda K

    The annual meeting of the Japanese Society for Comparative Endocrinology, 8th International Sympojium on Amphibian and reptilian Endocrinology and Neurobiology., Nov. 2014

  • Investigating the differentiation time of interstitial cells in the fetal mouse testis.

    YASUDA Keiko; Maeda E; Nishio S; Okazaki N; Aoyama M; Tamotsu S; Yasuda K

    The annual meeting of the Japanese Society for Comparative Endocrinology, 8th International Sympojium on Amphibian and reptilian Endocrinology and Neurobiology., Nov. 2014

  • Intense laser-plasma soft-X-ray sources and application\nfor biological X-ray microscopy

    YASUDA Keiko; Masataka Kado; Maki Kishimoto; Takeo Ejima; Satoshi Tamotsu; Keiko Yasuda; Masato Aoyama; Kunio Shinohara

    PLASMA2014, Nov. 2014, 新潟

  • 生きている細胞の内部構造を直接観察できる軟X線顕微鏡の開発

    YASUDA Keiko

    日本分子生物学会, Nov. 2014, 横浜, 横浜

  • HYBRID IMAGING OF LIVING BIOLOGICAL CELLS WITH A SOFT X-RAY MICROSCOPE AND A FLUORESCENT MICROSCOPE

    YASUDA Keiko; M. Kado; M. Kishimoto; S. Tamotsu; K. Yasuda; M. Aoyama; S. Tone; K. Shinohara

    12th International Conference on X-ray MIcroscopy, Oct. 2014, Melbourne Australia

  • Single flash imaging of live hydrated biological cells by a contact soft\nx-ray microscope coupled with an intense laser-plasma soft x-ray\nsource

    YASUDA Keiko; Kado M; Kishimoto M; Tamotsu S; Yasuda K; Aoyama M; Shinohara K

    18th International Microscopy Congress, Sep. 2014, Prague, Czech Republic

  • マウス卵巣におけるプロキネチシン(PK)およびその受容体の局在

    YASUDA Keiko

    日本動物学会第85回大会, Sep. 2014

  • 試料周辺の水の層の厚さの制御による密着型軟X線顕微鏡\nの高コントラスト化

    YASUDA Keiko

    日本応用物理学会第75回大会, Sep. 2014, 北海道

  • N‐K吸収端近傍における生物細胞内構造のコントラスト

    YASUDA Keiko

    応用物理学会秋季学術講演会, Sep. 2014

  • マウス精巣ライディッヒ細胞の顕微分光測定

    YASUDA Keiko

    日本放射光学会年会・放射光科学合同シンポジウム, Jan. 2014

  • マウスにおいてタキキニンは二次卵胞成長を促進する

    YASUDA Keiko

    日本比較内分泌学会38回大会, Oct. 2013

  • マウス精巣における新生仔期アンドロゲン投与による成体型ライディッヒ細胞増殖促進作用と成体型ライディヒ細胞の起源

    YASUDA Keiko

    日本比較内分泌学会第38回大会, Oct. 2013

  • マウス精巣におけるProkineticinとProkineticin受容体の局在について

    YASUDA Keiko

    日本比較内分泌学会第38回大会, Oct. 2013

  • マウスにおいてタキキニンは卵胞成長を促進する

    YASUDA Keiko

    日本動物学会第84回大会, Sep. 2013

  • マウス精巣ライディッヒ細胞の分光顕微観察

    YASUDA Keiko

    応用物理学会東北支部学術講演会, Dec. 2012

  • 新生仔マウス精巣の_x000B_成体型ライディッヒ前駆細胞の増殖に対する_x000B_アンドロゲンの作用時期

    YASUDA Keiko

    日本動物学会第83回大会, Sep. 2012

  • マウスにおいてタキキニンは卵細胞成長を促進する

    YASUDA Keiko

    日本動物学会第83回大会, Sep. 2012, 大阪大学

  • In situ observation of cellular organelles with a contact x-ray microscope

    YASUDA Keiko; M Kado; M Kishimoto; S Tamotsu; K Yasuda; K Shinohara

    XRM, Aug. 2012

  • Chemical shift images of Organella in Leydig cells of mice testis.

    YASUDA Keiko; T.Ejima; Y. Neichi; M. Yanaguihara; M.Kado; M.Ishino; K.Yasuda; S. Tamotsu

    XRM, Aug. 2012

  • 密着型X線顕微鏡によるマウスライディヒ細胞の吸収スペクトル

    YASUDA Keiko

    日本応用物理学会, 2012

  • タキキニンはマウス二次卵胞の成長を促進する

    YASUDA Keiko

    日本比較内分泌学会第37回大会, 2012, 福井

  • マウス卵巣の卵胞発育過程で起こる莢膜細胞層形成機構の解明V:卵巣内の間質細胞の集合

    YASUDA Keiko

    (社)日本動物学会第82回大会, Sep. 2011, (社)日本動物学会, 北海道旭川市

  • マウス卵巣顆粒膜細胞の増殖にTNFαはTNF receptor Iを介して作用するか?

    YASUDA Keiko

    (社)日本動物学会第82回大会, Sep. 2011, (社)日本動物学会, 北海道旭川市

  • レーザープラズマ軟X線顕微鏡による細胞内器官の構造解析II

    YASUDA Keiko

    日本物理学会2011年秋季大会, Sep. 2011, (社)日本物理学会, 富山

  • Development of single shot soft x-ray contact microscopy sustem for neno-scale dynamics measurement of living biological specimen

    YASUDA Keiko; Maki Kishimoto; Masataka Kado; Masahihiko Ishono; Satoshi Tamotsu; Keiko Yasuda; Kunio Shinohara

    The 3rd International Symposium " Laser-Driven Relativistic Plasma Applied to Science, Energy, Industry, and Medicine.", May 2011, Kyoto Japan

  • 細胞内器官の蛍光顕微鏡像と軟X線顕微鏡像との直接比較

    YASUDA Keiko

    光量子科学研究シンポジウム, Jun. 2010

  • マウス卵巣における卵胞選別機構の解明に向けてー抗アポトーシス因子cFLIPの発現に着目してー

    水平遥子; 青山雅人; 保智己; 安田恵子

    第44回日本比較内分泌学会大会及びシンポジウム, 09 Nov. 2019, 08 Nov. 2019, 10 Nov. 2019

  • マウス卵胞の莢膜細胞層形成過程に対するKit-ligand の関与

    近藤景子; 伊丹沙織; 村木彩香; 青山雅人; 保智己; 安田恵子

    第44回日本比較内分泌学会大会及びシンポジウム, 09 Nov. 2019, 08 Nov. 2019, 10 Nov. 2019

  • マウス卵巣において卵胞刺激ホルモン(FSH)の刺激によってマクロファージが産生するインターフェロンα7の卵胞発育に対する作用

    山本万遥; 鍛祥子; 青山雅人; 保智己; 安田恵子

    日本動物学会第90回大阪大会, 20 Sep. 2019, 12 Sep. 2019, 14 Sep. 2019

  • 莢膜/間質細胞特異的遺伝子の同定とゴナドトロピン非依存段階の卵巣内卵胞の成長における生物学的役割

    青山雅人; 白石慧; 松原伸; 堀江郁; 大杉知裕; 奥田利美; 安田恵子; 佐竹炎

    日本動物学会第90回大阪大会, 14 Sep. 2019, 12 Sep. 2019, 14 Sep. 2019

  • マウス卵巣顆粒膜細胞におけるFas/Fas ligandのアポトーシス誘導作用

    中野奏子; 岡田佐和子; 青山雅人; 保智己; 安田恵子

    日本動物学会第90回大阪大会, 12 Sep. 2019, 12 Sep. 2019, 14 Sep. 2019

Association Memberships

  • 日本動物学会

  • 日本発生生物学会

  • 日本比較内分泌学会

  • 日本内分泌学会

  • 日本生殖内分泌学会

Works

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    Apr. 2017, Mar. - 2018

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    Apr. 2016, Mar. - 2017

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    Apr. 2015, Mar. - 2016

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    Apr. 2014, Mar. - 2015

  • 高輝度軟X 線源を用いた軟X 線顕微鏡による細胞内器官の高解像観察

    Apr. 2014, Mar. - 2015

  • 軟X線による生きている細胞の生理現象の観察

    Apr. 2014, Mar. - 2015

  • マウス精巣ライディッヒ細胞の顕微分光測定

    Apr. 2014, Mar. - 2015

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    Apr. 2014, Mar. - 2015

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    Apr. 2013, Mar. - 2014

  • ペプチド性伝達分子が制御するマウス卵胞発育の新機構の解明

    Apr. 2012, Mar. - 2013



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