Refereed, The journal of physical chemistry. B, Balance between Association and Dissociation Rate Constants Determines Morphology and Property of Amyloid Fibrils., Taiga Nakagawa; Daisuke Tsuri; Ichiro Nishii; Naoko Kajimura; Katsumi Matsuzaki; Masaru Hoshino, A parallel dimeric Aβ(1-40) peptide was prepared, and its structural and fibrillogenic characteristics were examined. The covalent linking of the peptide strongly facilitated the spontaneous formation of thioflavin-T-active, fibrillar aggregates rich in β strands without a lag phase. However, the aggregates formed by the dimeric peptide did not exhibit "seeding activity" to catalyze the formation of amyloid fibrils by wild-type Aβ(1-40) molecules. Heteronuclear NMR analysis revealed that an isolated dimeric molecule in reverse micelles lacked ordered secondary structures. It was therefore considered that excessively high hydrophobicity caused by dimerization was the major reason for the rapid formation of amorphous aggregates without seeding activity. A hundred-fold dilution of the concentration of dimeric peptides reproduced the aggregation kinetics with a preceding lag phase of several hours, similar to that of wild-type molecules. The resulting aggregates exhibited a typical amyloid fibril-like morphology and, importantly, possessed seeding activity for wild-type peptides. The present results emphasize the importance of an appropriate balance between association and dissociation rate constants for the formation of "one-dimensional crystalline" amyloid fibrils., 12 Dec. 2024, 128, 49, 12325, 12332, Scientific journal, True, 10.1021/acs.jpcb.4c07654

Refereed, Journal of plant research, Isolation and characterization of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase gene from Botryococcus braunii, race B., Hidenobu Uchida; Koremitsu Sumimoto; Tomoka Oki; Ichiro Nishii; Eiichi Mizohata; Shigeki Matsunaga; Shigeru Okada, The B race of a green microalga Botryococcus braunii Kützing produces triterpene hydrocarbons that is a promising source for biofuel. In this algal race, precursors of triterpene hydrocarbons are provided from the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. The terminal enzyme of this pathway, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR) is regarded as one of the key enzymes that affect yields of products in terpene biosynthesis. In order to better understand the MEP pathway of the alga, cDNA and genomic clones of HDR were obtained from B. braunii Showa strain. B. braunii HDR (BbHDR) is encoded on a single copy gene including a 1509-bp open reading frame that was intervened by 6 introns. The exon-intron structure of BbHDR genes did not show clear relation to phylogeny, while its amino acid sequence reflected phyla and classes well. BbHDR sequence was distinctive from that of the HDR protein from Escherichia coli in the residues involved in hydrogen-bond network that surrounds substrate. Introduction of BbHDR cDNA into an E. coli HDR deficient mutant resulted in recovery of its auxotrophy. BbHDR expression level was upregulated from the onset of liquid culture to the 24th day after inoculation with a 2.5-fold increase and retained its level in the subsequent period., Sep. 2018, 131, 5, 839, 848, Scientific journal, False, 10.1007/s10265-018-1039-4

Refereed, Planta, Springer Verlag, 3D reconstruction of endoplasmic reticulum in a hydrocarbon-secreting green alga, Botryococcus braunii (Race B), Reiko Suzuki; Ichiro Nishii; Shigeru Okada; Tetsuko Noguchi, 01 Mar. 2018, 247, 3, 663, 677, Scientific journal, 10.1007/s00425-017-2811-8

Refereed, G3-GENES GENOMES GENETICS, Sequence of the Gonium pectorale Mating Locus Reveals a Complex and Dynamic History of Changes in Volvocine Algal Mating Haplotypes, Takashi Hamaji; Yuko Mogi; Patrick J. Ferris; Toshiyuki Mori; Shinya Miyagishima; Yukihiro Kabeya; Yoshiki Nishimura; Atsushi Toyoda; Hideki Noguchi; Asao Fujiyama; Bradley J. S. C. Olson; Tara N. Marriage; Ichiro Nishii; James G. Umen; Hisayoshi Nozaki, May 2016, 6, 5, 1179, 1189, Scientific journal, 10.1534/g3.115.026229

Refereed, PLANT AND CELL PHYSIOLOGY, A Receptor-Like Kinase, Related to Cell Wall Sensor of Higher Plants, is Required for Sexual Reproduction in the Unicellular Charophycean Alga, Closterium peracerosum-strigosum-littorale Complex, Naoko Hirano; Yuka Marukawa; Jun Abe; Sayuri Hashiba; Machiko Ichikawa; Yoichi Tanabe; Motomi Ito; Ichiro Nishii; Yuki Tsuchikane; Hiroyuki Sekimoto, Jul. 2015, 56, 7, 1456, 1462, Scientific journal, 10.1093/pcp/pcv065

Refereed, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, Colony sheath formation is accompanied by shell formation and release in the green alga Botryococcus braunii (race B), Yuki Uno; Ichiro Nishii; Satoshi Kagiwada; Tetsuko Noguchi, Mar. 2015, 8, 214, 223, Scientific journal, 10.1016/j.algal.2015.02.015

Refereed, PLoS ONE, Transformation of lipid bodies related to hydrocarbon accumulation in a green alga, Botryococcus braunii (race B), Reiko Suzuki; Naoko Ito; Yuki Uno; Ichiro Nishii; Satoshi Kagiwada; Sigeru Okada; Tetsuko Noguchi, 05 Dec. 2013, 8, 12, e81626, Scientific journal, 10.1371/journal.pone.0081626

Refereed, PLoS ONE, Distribution of the Sex-Determining Gene MID and Molecular Correspondence of Mating Types within the Isogamous Genus Gonium (Volvocales, Chlorophyta), Takashi Hamaji; Patrick J. Ferris; Ichiro Nishii; Yoshiki Nishimura; Hisayoshi Nozaki, 16 May 2013, 8, 5, e64385, Scientific journal, 10.1371/journal.pone.0064385

Refereed, PLOS ONE, Mitochondrial and Plastid Genomes of the Colonial Green Alga Gonium pectorale Give Insights into the Origins of Organelle DNA Architecture within the Volvocales, Takashi Hamaji; David R. Smith; Hideki Noguchi; Atsushi Toyoda; Masahiro Suzuki; Hiroko Kawai-Toyooka; Asao Fujiyama; Ichiro Nishii; Tara Marriage; Bradley J. S. C. Olson; Hisayoshi Nozaki, Feb. 2013, 8, 2, e57177, Scientific journal, 10.1371/journal.pone.0057177

Refereed, CRYOLETTERS, AMIDIC AND ACETONIC CRYOPROTECTANTS IMPROVE CRYOPRESERVATION OF VOLVOCINE GREEN ALGAE, Atsushi Nakazawa; Ichiro Nishii, May 2012, 33, 3, 202, 213, Scientific journal

Not Refereed, PHYCOLOGIA, Embryogenesis and cell positioning in Platydorina caudata (Volvocaceae, Chlorophyta), Hitoshi Iidai; Ichiro Nishii; Sao Inuye, Sep. 2011, 50, 5, 530, 540, Scientific journal, 10.2216/10-80.1

Not Refereed, CURRENT OPINION IN PLANT BIOLOGY, Volvox: Simple steps to developmental complexity?, Ichiro Nishii; Stephen M. Miller, Dec. 2010, 13, 6, 646, 653, 10.1016/j.pbi.2010.10.005

Not Refereed, SCIENCE, Genomic Analysis of Organismal Complexity in the Multicellular Green Alga Volvox carteri, Simon E. Prochnik; James Umen; Aurora M. Nedelcu; Armin Hallmann; Stephen M. Miller; Ichiro Nishii; Patrick Ferris; Alan Kuo; Therese Mitros; Lillian K. Fritz-Laylin; Uffe Hellsten; Jarrod Chapman; Oleg Simakov; Stefan A. Rensing; Astrid Terry; Jasmyn Pangilinan; Vladimir Kapitonov; Jerzy Jurka; Asaf Salamov; Harris Shapiro; Jeremy Schmutz; Jane Grimwood; Erika Lindquist; Susan Lucas; Igor V. Grigoriev; Ruediger Schmitt; David Kirk; Daniel S. Rokhsar, Jul. 2010, 329, 5988, 223, 226, Scientific journal, 10.1126/science.1188800

Not Refereed, SCIENCE, Evolution of an Expanded Sex-Determining Locus in Volvox, Patrick Ferris; Bradley J. S. C. Olson; Peter L. De Hoff; Stephen Douglass; David Casero; Simon Prochnik; Sa Geng; Rhitu Rai; Jane Grimwood; Jeremy Schmutz; Ichiro Nishii; Takashi Hamaji; Hisayoshi Nozaki; Matteo Pellegrini; James G. Umen, Apr. 2010, 328, 5976, 351, 354, Scientific journal, 10.1126/science.1186222

Refereed, JOURNAL OF PHYCOLOGY, IDENTIFICATION OF THE MINUS MATING-TYPE SPECIFIC GENE MTD1 FROM GONIUM PECTORALE (VOLVOCALES, CHLOROPHYTA)1, Takashi Hamaji; Patrick J. Ferris; Ichiro Nishii; Hisayoshi Nozaki, Dec. 2009, 45, 6, 1310, 1314, Scientific journal, 10.1111/j.1529-8817.2009.00744.x

Not Refereed, PLANT CELL, Controlled Enlargement of the Glycoprotein Vesicle Surrounding a Volvox Embryo Requires the InvB Nucleotide-Sugar Transporter and Is Required for Normal Morphogenesis, Noriko Ueki; Ichiro Nishii, Apr. 2009, 21, 4, 1166, 1181, Scientific journal, 10.1105/tpc.109.066159

Not Refereed, KAGAKU TO SEIBUTSU, Japan Society for Bioscience, Biotechnology, and Agrochemistry, Molecular Mechanism of Morphogenesis in Volvox Embryos, NISHII Ichiro, 2009, 47, 3, 176, 184, 10.1271/kagakutoseibutsu.47.176

Refereed, GENETICS, Idaten Is a New Cold-Inducible Transposon of Volvox carteri That Can Be Used for Tagging Developmentally Important Genes, Noriko Ueki; Ichiro Nishii, Nov. 2008, 180, 3, 1343, 1353, Scientific journal, 10.1534/genetics.108.094672

Refereed, GENETICS, Identification of the minus-dominance gene ortholog in the mating-type locus of Gonium pectorale, Takashi Hamaji; Patrick J. Ferris; Annette W. Coleman; Sabine Waffenschmidt; Fumio Takahashi; Ichiro Nishii; Hisayoshi Nozaki, Jan. 2008, 178, 1, 283, 294, Scientific journal, 10.1534/genetics.107.078618

Not Refereed, 2008, 56, 25, 28

Refereed, JOURNAL OF MOLECULAR EVOLUTION, The VARL gene family and the evolutionary origins of the master cell-type regulatory gene, regA, in Volvox carteri, Leonard Duncan; Ichiro Nishii; Alexandra Harryman; Stephanie Buckley; Alicia Howard; Nicholas R. Friedman; Stephen M. Miller, Jul. 2007, 65, 1, 1, 11, Scientific journal, 10.1007/s00239-006-0225-5

Refereed, CURRENT GENETICS, Orthologs and paralogs of regA, a master cell-type regulatory gene in Volvox carteri, L Duncan; Nishii, I; A Howard; D Kirk; SM Miller, Jul. 2006, 50, 1, 61, 72, Scientific journal, 10.1007/s00294-006-0071-4

Not Refereed, 2004, 49, 9, 1253, 1264

Refereed, CELL, A kinesin, InvA, plays an essential role in Volvox morphogenesis, Nishii, I; S Ogihara; DL Kirk, Jun. 2003, 113, 6, 743, 753, Scientific journal, 10.1016/S0092-8674(03)00431-8

Not Refereed, 2003, 21, 2557, 2559

Not Refereed, 2003, 39, 80, 85

Not Refereed, DEVELOPMENT GROWTH & DIFFERENTIATION, Volvox carteri as a model for studying the genetic and cytological control of morphogenesis, DL Kirk; Nishii, I, Dec. 2001, 43, 6, 621, 631, 10.1046/j.1440-169X.2001.00612.x

Refereed, JOURNAL OF MOLECULAR BIOLOGY, Identification of the phospholipid-binding site of human beta(2)-glycoprotein I domain V by heteronuclear magnetic resonance, M Hoshino; Y Hagihara; Nishii, I; T Yamazaki; H Kato; Y Goto, Dec. 2000, 304, 5, 927, 939, Scientific journal, 10.1006/jmbi.2000.4243

Refereed, DEVELOPMENT, Actomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo, Nishii, I; S Ogihara, May 1999, 126, 10, 2117, 2127, Scientific journal

Refereed, JOURNAL OF MOLECULAR BIOLOGY, THERMODYNAMIC STABILITY OF THE MOLTEN GLOBULE STATES OF APOMYOGLOBIN, NISHII, I; M KATAOKA; Y GOTO, Jul. 1995, 250, 2, 223, 238, Scientific journal, 10.1006/jmbi.1995.0373

Refereed, JOURNAL OF MOLECULAR BIOLOGY, STRUCTURAL CHARACTERIZATION OF THE MOLTEN GLOBULE AND NATIVE STATES OF APOMYOGLOBIN BY SOLUTION X-RAY-SCATTERING, M KATAOKA; NISHII, I; T FUJISAWA; T UEKI; F TOKUNAGA; Y GOTO, May 1995, 249, 1, 215, 228, Scientific journal, 10.1006/jmbi.1995.0290

Refereed, BIOCHEMISTRY, COLD DENATURATION OF THE MOLTEN GLOBULE STATES OF APOMYOGLOBIN AND A PROFILE FOR PROTEIN-FOLDING, NISHII, I; M KATAOKA; F TOKUNAGA; Y GOTO, Apr. 1994, 33, 16, 4903, 4909, Scientific journal, 10.1021/bi00182a019

Refereed, BIOCHEMISTRY, ACID-INDUCED UNFOLDING AND REFOLDING TRANSITIONS OF CYTOCHROME-C - A 3-STATE MECHANISM IN H2O AND D2O, Y GOTO; Y HAGIHARA; D HAMADA; M HOSHINO; NISHII, I, Nov. 1993, 32, 44, 11878, 11885, Scientific journal, 10.1021/bi00095a017

Not Refereed, PHYCOLOGIA, BIOSYNTHESIS AND ACCUMULATION OF HYDROCARBONS AND POLYSACCHARIDES IN A COLONIAL GREEN ALGA, BOTRYOCOCCUS BRAUNII RACE B, R. Suzuki; Y. Uno; I. Nishii; S. Kagiwada; T. Noguchi, Jul. 2013, 52, 4, 109, 109, Summary international conference

Not Refereed, PHYCOLOGIA, MORPHOGENESIS IN THE ASEXUAL EMBRYO OF PLATYDORINA CAUDATA (CHLOROPHYCEAE), H. Iida; I. Nishii; I. Inouye, Jul. 2009, 48, 4, 47, 47, Summary international conference

Not Refereed, PHYCOLOGIA, GENOMIC ANALYSIS ON MATING TYPE LOCI IN THE COLONIAL VOLVOCALEAN ALGAE GONIUM PECTORALE AND PLEODORINA STARRII, T. Hamaji; I. Nishii; H. Nozaki, Jul. 2009, 48, 4, 38, 38, Summary international conference

Not Refereed, PHYCOLOGIA, INVD, A NOVEL PHOSPHOPROTEIN, IS INVOLVOED IN CELL SHAPE CHANGE IN MORPHOGENESIS OF VOLVOX EMBRYO, H. Toyooka; K. Ishida; A. Nakazawa; H. Sekimoto; H. Nozaki; I. Nishii, Jul. 2009, 48, 4, 131, 131, Summary international conference

Not Refereed, GENES & GENETIC SYSTEMS, Characterization of genes required for morphogenesis of Volvox embryo, Ichiro Nishii; Hiroko Toyooka; Noriko Ueki; Jun Kadota; Koichi Ishida, Dec. 2007, 82, 6, 513, 513, Summary international conference

Not Refereed, JOURNAL OF PHYCOLOGY, Molecular analysis on the mating type locus of gonium pectorale (volvocales, chlorophyta), T. Hamaji; F. Takahashi; Nishii, I; H. Nozaki, Dec. 2007, 43, 3, 4, Summary international conference

Not Refereed, MOLECULAR BIOLOGY OF THE CELL, A kinesin-like protein encoding gene, invA, is required for the cellular movements that drive inversion of Volvox embryos, Nishii, I; DL Kirk, Nov. 2002, 13, 149A, 149A, Summary international conference

Not Refereed, DEVELOPMENTAL BIOLOGY, The invA gene of Volvox encodes a novel kinesin that is required for inversion of the embryo., Nishii, I; DL Kirk, Jul. 2001, 235, 1, 197, 197, Summary international conference

Not Refereed, MOLECULAR BIOLOGY OF THE CELL, Role of actomyosin in multicellular deformation: Inversion of Volvox embryos., Nishii, I; S Ogihara, Dec. 1996, 7, 2219, 2219, Summary international conference

2005, Not Refereed, 0387360581

1999, Not Refereed

Poster presentation, 23 Mar. 2025, 21 Mar. 2025 - 24 Mar. 2025, Japan, False

Poster presentation, 23 Mar. 2024, 22 Mar. 2024 - 25 Mar. 2024

Toshinobu Suzaki; Hideki Ishida; Maho Shimada; Kousei Yamamoto; Liudmyla Gaponova; Hideyuki Iriko; Terue Harumoto; Yasuhiro Fukuda; Ichiro Nishii; Mikihiko Arikawa; Masashi M. Hayakawa; Rina Higuchi, Application of water freeze-drying method to biological materials, Oral presentation, 17 Sep. 2023, 15 Sep. 2023 - 17 Sep. 2023

R. Suzuki; Y. Uno; I. Nishii; S. Kagiwada; T. Noguchi, PHYCOLOGIA, BIOSYNTHESIS AND ACCUMULATION OF HYDROCARBONS AND POLYSACCHARIDES IN A COLONIAL GREEN ALGA, BOTRYOCOCCUS BRAUNII RACE B, Jul. 2013, Jul. 2013 - Jul. 2013, INT PHYCOLOGICAL SOC

Vienna Biocenter PhD Symposium ‘THINK ALTERNATIVE Insights from Unconventional model organisms’, Volvox as a simple model for elucidating the evolution of morphogenesis in the early stage, 2011

JSDB-GFE Joint meeting of Developmental Biology, Dresden, Germany, Tracing evolutionary steps of morphogenesis from Chlamydomonas to Volvox, 2011

The 3rd NIBB-TLL-MPIZ Joint Symposium 2011 “Cell Cycle and Development”, Evolution and Development of Volvox, 2011

Mechanics of Cells and Tissues: Sensing, Generating and Coordinating Forces in Biological Systems (Organizers: J. Howard & J. Theriot) Boehringer Ingelheim Fonds International Titisee Conferences, Titisee, Germany, Morphogenesis in green algae: how Volvox embryo turns inside-out, 2010

International GCOE Symposium Morphogenesis and Signaling - From Proteins, Organelles to Organisms - (Organizers: K. Hatta, H. Yoshida, H. Nishitani, M. Sakaguchi), Cell shape change and cell migration turn embryos inside-out in morphogenesis of Volvox, 2010

H. Toyooka; K. Ishida; A. Nakazawa; H. Sekimoto; H. Nozaki; I. Nishii, PHYCOLOGIA, INVD, A NOVEL PHOSPHOPROTEIN, IS INVOLVOED IN CELL SHAPE CHANGE IN MORPHOGENESIS OF VOLVOX EMBRYO, Jul. 2009, Jul. 2009 - Jul. 2009, INT PHYCOLOGICAL SOC

T. Hamaji; I. Nishii; H. Nozaki, PHYCOLOGIA, GENOMIC ANALYSIS ON MATING TYPE LOCI IN THE COLONIAL VOLVOCALEAN ALGAE GONIUM PECTORALE AND PLEODORINA STARRII, Jul. 2009, Jul. 2009 - Jul. 2009, INT PHYCOLOGICAL SOC

H. Iida; I. Nishii; I. Inouye, PHYCOLOGIA, MORPHOGENESIS IN THE ASEXUAL EMBRYO OF PLATYDORINA CAUDATA (CHLOROPHYCEAE), Jul. 2009, Jul. 2009 - Jul. 2009, INT PHYCOLOGICAL SOC

EMBO Conference Series on Morphogenesis and Dynamics of Multicellular Systems, European Molecular Biology Laboratory, Heidelberg, Germany, Genes controlling cell shape change, migration and ECM required for turning Volvox embryo inside-out, 2009

The 13th International Conference of Cellular and Molecular Biology on Chlamydomonas (EMBO Workshop),, invD and invE genes control the change in cell shape that is required for inversion of Volvox embryos, 2008

T. Hamaji; F. Takahashi; Nishii, I; H. Nozaki, JOURNAL OF PHYCOLOGY, Molecular analysis on the mating type locus of gonium pectorale (volvocales, chlorophyta), Dec. 2007, Dec. 2007 - Dec. 2007, BLACKWELL PUBLISHING

Ichiro Nishii; Hiroko Toyooka; Noriko Ueki; Jun Kadota; Koichi Ishida, GENES & GENETIC SYSTEMS, Characterization of genes required for morphogenesis of Volvox embryo, Dec. 2007, Dec. 2007 - Dec. 2007, GENETICS SOC JAPAN

Volvox Meeting, Idaten, a novel transposon, has been used to identify a gene controlling the change in cell shape that is required for inversion of Volvox embryos, 2007

The 12th International Conference on the Cell and Molecular Biology of Chlamydomonas, Recent progress in Volvox research and expanding the molecular understanding of inversion of the V. carteri embryo, 2006

日本分子生物学会2006フォーラム「分子生物学の未来」シンポジウムBehavior of Individual Cells during Morphogenesis, Isolation and characterization of mutants deficient in morphogenesis of the Volvox embryo, 2006

NAIST-CDB 国際シンポジウム「分節研究から発生生物学の普遍原理へ」（オーガナイザー 奈良先端大学 高橋淑子）, Cellular and molecular mechanism of cell-sheet folding process in inversion of Volvox embryo, 2005

Nishii, I; DL Kirk, MOLECULAR BIOLOGY OF THE CELL, A kinesin-like protein encoding gene, invA, is required for the cellular movements that drive inversion of Volvox embryos, Nov. 2002, Nov. 2002 - Nov. 2002, AMER SOC CELL BIOLOGY

Workshop“Building the Cell II: Development of Complex Structures in Simple Cells”, American Society For Cell Biology, 2002 Annual Meeting, San Francisco, USA, Folding a multicellular sheet in the morphogenesis of Volvox, 2002

Minisymposium “Cytoskeletal Processes During Development" American Society For Cell Biology, 2002 Annual Meeting, San Francisco, USA, A kinesin-like protein is required for the cellular movements that drive inversion of Volvox embryos”, 2002

Nishii, I; DL Kirk, DEVELOPMENTAL BIOLOGY, The invA gene of Volvox encodes a novel kinesin that is required for inversion of the embryo., Jul. 2001, Jul. 2001 - Jul. 2001, ACADEMIC PRESS INC

Nishii, I; S Ogihara, MOLECULAR BIOLOGY OF THE CELL, Role of actomyosin in multicellular deformation: Inversion of Volvox embryos., Dec. 1996, Dec. 1996 - Dec. 1996, AMER SOC CELL BIOLOGY