MAEDA Sumio

researchmap

Profile and Settings

• Name (Japanese)

  Maeda

• Name (Kana)

  Sumio

Research Interests

• ＜研究室web → https://www.nara-wu.ac.jp/life/food/microbiol_and_hyg/maedalabo.html＞
• 抗生物質耐性菌・耐性遺伝子
• パーシスター細胞
• 大腸菌
• バイオフィルム
• 遺伝子水平伝播
• 微生物
• persister
• antibiotic-resistant bacteria
• Escherichia coli
• biofilm
• horizontal gene transfer
• microbiology

Research Areas

• Life sciences, Genetics
• Life sciences, Bacteriology
• Life sciences, Applied microbiology
• Life sciences, Applied molecular and cellular biology

Association Memberships

• 日本微生物生態学会
• 日本生化学会
• 日本分子生物学会
• 日本農芸化学会

Ⅱ．研究活動実績

Published Papers

• Refereed, Current Research in Microbial Sciences, Elsevier, Subminimal inhibitory concentrations of ampicillin and mechanical stimuli cooperatively promote cell-to-cell plasmid transformation in Escherichia coli, Sayuri Kasagaki; Mayuko Hashimoto; Sumio Maeda, Horizontal gene transfer (HGT) is a bacterial evolution tool for improved survival. Although several environmental stimuli induce or promote HGT, the diversity and complexity of the environmental factors have not been sufficiently elucidated. In this study, we showed that the biofilm culture of Escherichia coli at the air–solid interface in the presence of a subminimal inhibitory concentration (sub-MIC) of ampicillin (∼0.5–4 µg/mL) and subsequent mechanical stimulation (rolling small glass balls, ø = 5–8 mm) cooperatively promoted horizontal plasmid transfer without the usual competence-inducing conditions. Either of the two treatments promoted plasmid transfer at a lower frequency than when the treatments were combined. The effect of several parameters on the two treatments was tested and then optimized, achieving a high frequency of plasmid transfer (up to 10−6 per cell) under optimal conditions. Plasmid transfer was DNase-sensitive for both treatments, demonstrating its mechanism of transformation. Plasmid transfer occurred using various E. coli strains, plasmids, ball materials, shaking conditions, and even the mechanical stimulation of brushing the biofilm with a toothbrush, indicating the conditional flexibility of this phenomenon. This is the first demonstration of the promoting effect of the combination of a sub-MIC antibiotic and mechanical stimulation on horizontal plasmid transfer between E. coli cells via transformation. Regarding environmental bacterial physiology, the aggregations or biofilms of bacterial cells may encounter both sub-MIC antibiotics and mechanical stimuli in some specific environments, therefore, this type of HGT could also occur naturally., Apr. 2022, 3, 100130, Scientific journal, True, 10.1016/j.crmicr.2022.100130

  PermalinkDOI URL
• Refereed, World Journal of Microbiology and Biotechnology, Springer Science and Business Media LLC, Bovine serum promotes the formation and phenotype memory retention of persister cells in Escherichia coli liquid cultures, Erika Suzuki; Tomoka Urushidani; Sumio Maeda, Jul. 2021, 37, 7, Scientific journal, 10.1007/s11274-021-03073-8

  PermalinkURLDOI URL共同研究・競争的資金等URL
• Not Refereed, bioRxiv, Cold Spring Harbor Laboratory, First and broad detection of three typical carbapenemase genes on the surfaces of commercially available spices worldwide and isolation of complete NDM-1 genes from black pepper, cumin, and clove, Minako Mochizuki; Sumio Maeda, The spread of multidrug-resistant bacteria, particularly those producing carbapenemases, has become a major public health concern. The presence of carbapenemase genes has primarily been reported in clinical samples, whereas the presence of these genes in commercially available foods has insufficiently been studied despite its growing importance. The present study aimed to detect and characterize carbapenemase genes (bla_NDM,bla_IMP, bla_KPC, and bla_OXA-48-like) on the surfaces of commercially available spices using PCR to amplify conserved regions of these genes. It was revealed that DNAs of these genes are commonly present (13 genes/29 samples) on spices derived from at least 9 different countries. This is the first detection of any carbapenemase gene on eight spices (black pepper, cumin, clove, cardamom, mustard, caraway, parsley, and rosemary) among these. This is also the first detection of the bla_IMP and bla_NDM as well as the broad detection of the bla_OXA-48-like on spices. We also isolated complete, functional bla_NDM-1 genes from three spices (black pepper, cumin, and clove) up to the present., 10 Jun. 2020, 2020, bioRxiv, Scientific journal, 10.1101/2020.06.09.141614

  PermalinkDOI URL
• Refereed, Methods in Molecular Biology "Horizontal Gene Transfer", Springer US, Natural Transformation in Escherichia coli, Yoko Komiyama; Sumio Maeda, 2020, 2075, 179, 187, In book, 10.1007/978-1-4939-9877-7_13

  PermalinkDOI URL共同研究・競争的資金等URL
• Refereed, Biochemical and Biophysical Research Communications, High temperatures promote cell-to-cell plasmid transformation in Escherichia coli, Hashimoto M; Hasegawa H; MAEDA Sumio, Jun. 2019, 515, 196
• Refereed, Frontiers in Microbiology, Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms, Hasegawa H; Suzuki E; Maeda S, Oct. 2018, 9, 2365

  共同研究・競争的資金等URL
• Refereed, Frontiers in Microbiology, Bacterial memory of persisters: Bacterial persister cells can retain their phenotype for days or weeks after withdrawal from colony–biofilm culture, Saki Miyaue; Erika Suzuki; Yoko Komiyama; Yu Kondo; Miki Morikawa; Sumio Maeda, 2018, 9, 1396, 10.3389/fmicb.2018.01396

  DOI URL共同研究・競争的資金等URL
• Refereed, AIMS Microbiology, Bacteriophage P1vir-induced cell-to-cell plasmid transformation in Escherichia coli, Chiaki Sugiura; Saki Miyaue; Yuka Shibata; Akiko Matsumoto; Sumio Maeda, 2017, 3, 4, 784, 797, 10.3934/microbiol.2017.4.784

  DOI URL共同研究・競争的資金等URL
• Refereed, Microbes in the spotlight: recent progress in the understanding of beneficial and harmful microorganisms, Competence development and horizontal plasmid transfer in natural Escherichia coli strains, Akiko Matsumoto; Ayuka Sekoguchi; Yukako Murakami; Junko Imai; Kumiko Kondo; Yuka Shibata; Sumio Maeda, 2016, 468, 473

  共同研究・競争的資金等URL
• Refereed, Biochemical and Biophysical Research Communications, Natural Escherichia coli strains undergo cell-to-cell plasmid transformation, Akiko Matsumoto; Ayuka Sekoguchi; Junko Imai; Kumiko Kondo; Yuka Shibata; Sumio Maeda, 2016, 481, 59, 62, 10.1016/j.bbrc.2016.11.018

  DOI URL共同研究・競争的資金等URL
• Refereed, 家政学研究, 大腸菌標準野生株（ECOR strains）におけるバイオフィルム形成能の分析, 柴田有加; 松本晃子; 世古口歩華; 前田純夫, 2014, 60, 2, 111, 116
• Refereed, Biochemical and Biophysical Research Communications, Genome-wide screen for Escherichia coli genes involved in repressing cell-to-cell transfer of non-conjugative plasmids, Matsuda, A; Kurono, N; Kawano, C; Shirota, K; Hirabayashi, A; Horino, M; Etchuya, R; Sobue, R; Sasaki, Y; Miyaue, S; Sekoguchi, A; Sugiura, C; Shibata, Y; Ito, M; Ando, T; Maeda, S, 2012, 428, 445, 450, 10.1016/j.bbrc.2012.10.098

  DOI URL共同研究・競争的資金等URL
• Refereed, Biochemical and Biophysical Research Communications, Genome-wide screening of Escherichia coli genes involved in execution and promotion of cell-to-cell transfer of non-conjugative plasmids: rodZ (yfgA) is essential for plasmid acceptance in recipient cells., Naomi Kurono; Ayako Matsuda; Rika Etchuya; Rina Sobue; Yumi Sasaki; Miki Ito; Tsuyako Ando; Sumio Maeda, 2012, 421, 119, 123, 10.1016/j.bbrc.2012.03.127

  DOI URL共同研究・競争的資金等URL
• Refereed, Peptide Science 2010, Genome-wide screening of Escherichia coli genes involved in repression of peptide-pheromone-regulated natural transformation, MAEDA Sumio; Ayako Matsuda; Naomi Kurono; Chinatsu Kawano; Kozue Shirota; Akiko Hirabayashi; Mutsumi Horino; Rika Etchuuya; Rina Sobue; Yumi Sasaki; Tsuyako Ando; Miki Ito; Sumio Maeda, 2011, 81
• Refereed, Peptide Science 2010, Genome-wide screening of Escherichia coli genes involved in exection and promotion of peptide-pheromone-regulated natural transformation, Naomi Kurono; Ayako Matsuda; Rika Etchuuya; Rina Sobue; Yumi Sasaki; Tsuyako Ando; Miki Ito; Sumio Maeda, 2011, 79
• Refereed, FEBS Letters, Identification of a novel DNA element that promotes cell-to-cell transformation in Escherichia coli., Rina Sobue; Naomi Kurono; Rika Etchuya; Sumio Maeda, 2011, 585, 2223, 2228, 10.1016/j.febslet.2011.05.040

  DOI URL共同研究・競争的資金等URL
• Refereed, PLoS One, Cell-to-Cell Transformation in Escherichia coli: A Novel Type of Natural Transformation Involving Cell-Derived DNA and a Putative Promoting Pheromone., MAEDA Sumio; Rika Etchuuya; Miki Ito; Seiko Kitano; Fukiko Shigi; Rina Sobue; Sumio Maed, 2011, 6, 1, e16355, 10.1371/journal.pone.0016355

  DOI URL共同研究・競争的資金等URL
• Refereed, World Journal of Microbiology and Biotechnology, Horizontal transfer of non-conjugative plasmid in colony biofilm of Escherichia coli on food-based media, Ando, T; Maeda, S, 2009, 25, 1865, 1869, 10.1007/s11274-009-0070-y

  DOI URL共同研究・競争的資金等URL
• Refereed, World Journal of Microbiology and Biotechnology, Freeze-thaw-induced lateral transfer of non-conjugative plasmids by in situ transformation in Escherichia coli in natural waters and food extracts, Ishimoto, Y; Kato, S; Maeda, S, 2008, 24, 11, 2731-2735, doi:10.1007/s11274-008-9761-z, 10.1007/s11274-008-9761-z

  DOI URL共同研究・競争的資金等URL
• Refereed, DNA多型, Phi29 DNA polymeraseで増幅した米ゲノムDNAのPCRによる遺伝子多型分析への適用例と条件検討, 伊藤他; 前田 純夫, 2007, 15, 299, 303
• Refereed, IFO Research Communications, バイオフィルム状微生物の遺伝子転移に関する研究, 前田 純夫, 2007, 21, 71, 81
• Refereed, DNA多型, Phi29 DNA polymeraseで増幅したヒト口腔粘膜細胞DNAのPCRによる遺伝子多型分析への適用例と条件検討, 伊藤未来; 井上幸子; 前田純夫, 2006, 14, 46, 51
• Not Refereed, 食品機械装置, 微生物バイオフィルムと食品製造環境, 前田純夫, 2006, 43, 48, 53
• Not Refereed, 日刊工業新聞, 食の安心を築くために, 前田純夫, 2006, 19871, 14
• Refereed, FEMS Microbiology Letters, Horizontal Transfer of Nonconjugative Plasmids in a Colony Biofilm of Escherichia coli., Maeda, S; Ito, M; Ando, T; Ishimoto, Y; Fujisawa, Y; Takahashi, H; Sawamura, A; Matsuda, A; Kato, S, 2006, 255, 115, 120, 10.1111/j.1574-6968.2005.00072.x

  DOI URL
• Refereed, 家政学研究, 遺伝子組換え食品の社会的受容過程の分析, 堺谷展子; 前田純夫, 2005, 52, 27, 38
• Refereed, 家政学研究, Cell-direct PCR法によるヒトゲノムSNPの簡便検出法, 石本裕子; 前田純夫, 2004, 51, 1, 5
• Refereed, 家政学研究, 奈良女子大学学生の遺伝子組換え食品への意識調査, 堺谷展子; 前田純夫, 2004, 50, 154, 162
• Refereed, FEMS Microbiology Letters, Transformation of Colonial Escherichia coli on Solid Media, Maeda, S; Sawamura, A; Matsuda, A, 2004, 236, 61, 64, 10.1016/j.femsle.2004.05.023

  DOI URL
• Refereed, Enzyme and Microbial Technology, Purification and Characterization of an Extracellular Laccase of a Fungus (Family Chaetomiaceae) Isolated from Soil., Saito, T; Hong, P; Kato, K; Okazaki, M; Inagaki, H; Tanaka, K; Takada, M; Maeda, S; Yokogawa, Y, 2003, 33, 520, 526, 10.1016/S0141-0229(03)00158-3

  DOI URL
• Not Refereed, 食物科学概論, バイオテクノロジーと食物, 前田 純夫, 2003, 6.3
• Refereed, Microbes & Environment, Competency Development of Escherichia coli in Foodstuffs., MAEDA Sumio, 2003, 18, 2, 100, 103, 10.1264/jsme2.18.100

  DOI URL
• Refereed, Cell Biology International, Spatial Distribution of mDLG6 mRNA in Embryonic and Adult Mouse Brain., Inagaki, H; Tanaka, K; Takada, M; Maeda, S; Ichihara, S; Saito, T, 2002, 26, 635, 640, 10.1006/cbir.2002.0922

  DOI URL
• Refereed, DNA Sequence, Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA, Inagaki, H; Hayashi, T; Matsushima, Y; Lin. K.H; Maeda, S; Ichihara, S; Kitagawa, Y; Saito, T, 2002, 11, 131, 135, 10.3109/10425170009033980

  DOI URL
• Refereed, 化学と工業, プロテイントランスダクション：外来タンパク質を細胞内に導入する新方法, 前田 純夫, 2000, 53, 4, 519
• Refereed, Bioscience, Biotechnology, and Biochemistry, Staurosporine Promotion of Formation of Continuous Monolayers of Primary Rat Hepatocytes by Improving Attachment and Spreading., MAEDA Sumio; Maeda, S; Lin, K.H; Inagaki, H; Saito, T, 2000, 64, 9, 1985, 1987, 10.1271/bbb.64.1985

  DOI URL
• Refereed, The Hepatocyte Review, Mechanisms of Active Cell Death in Isolated Hepatocytes, MAEDA Sumio, 2000, Chapter 18, 251, 267
• Refereed, Biochemical and Biophysical Research Communications, rDLG6: a Novel Homolog of Drosophila DLG Expressed in Rat Brain., Inagaki, H; Maeda, S; Lin, K.H; Shimizu, N; Saito, T, 1999, 265, 462, 468, 10.1006/bbrc.1999.1723

  DOI URL
• Refereed, Biochemistry and Molecular Biology International, Inhibition of Mitochondrial Gene Expression by Antisense RNA of Mitochondrial Transcription Factor A (mtTFA)., Inagaki, H; Kitano, S; Lin, K.H; Maeda, S; Saito, T, 1998, 45, 567, 573
• Refereed, Materials Sci. Eng. C, Influence of Aldehyde Groups on the Thermostability of an Immobilized Enzyme on an Inorganic Support., Saito, T; Yoshida, Y; Kawashima, K; Lin, K.H; Maeda, S; Kobayashi, T, 1997, 5, 149, 152
• Refereed, Bioscience, Biotechnology, and Biochemistry, Long-Term Culture of Rat Hepatocytes on Heparin- or Lambda Carrageenan- containing Collagen Gels, Lin, K.H; Maeda, S; Inagaki, H; Saito, T, 1997, 61, 971, 974, 10.1271/bbb.61.971

  DOI URL
• Not Refereed, 工業技術, アポトーシス（細胞の自殺）誘導機構の解明とその生物工学的利用, 前田 純夫, 1997, 38, 2, 33
• Not Refereed, KITEC INFORMATION, アポトーシス（細胞の自殺）誘導機構の解明とその生物工学的利用, 前田 純夫, 1997, 36, 4, 6
• Refereed, Journal of Biochemistry, Molecular Biology and Biophysics, Promotive Effect of DMSO on TGF-b1-Induced Apoptosis in Primary Culture of Rat Hepatocytes., MAEDA Sumio; Maeda, S; Miyazawa, A; Lin, K.H; Inagaki, H; Saito, T, 1997, 1, 117, 124
• Refereed, Life Sciences, Osteonectin Gene Expression in Fibrotic Liver., Inagaki, H; Lin, K.H; Maeda, S; Saito, T, 1996, 58, 927, 934, 10.1016/0024-3205(96)00035-5

  DOI URL
• Refereed, Biochemistry and Molecular Biology International, Induction of Apoptosis in Primary Culture of Rat Hepatocytes by Protease Inhibitors., Maeda, S; Lin, K.H; Inagaki, H; Saito, T, 1996, 39, 447, 453
• Refereed, Experimental Cell Research, Albumin Synthesis by Rat Hepatocytes Cultured on Collagen Gels Is Sustained Specifically by Heparin., Lin, K.H; Hino, H; Maeda, S; Inagaki, H; Valiakhmetov, A.J; Saito, T, 1995, 218, 717, 721, 10.1006/excr.1995.1283

  DOI URL
• Refereed, 名古屋工業技術研究所報告, 肝実質細胞と血管内皮細胞の相互作用による増殖と分化機能の発現制御に関する研究, 前田純夫; 林孔華; 稲垣英利; 斎藤隆雄, 1995, 44, 530, 546
• Refereed, Biotechnology and Applied Biochemistry, Long-Term Maintenance of Liver-Specific Functions in Three-Dimensional Culture of Adult Rat Hepatocytes with a Porous Gelatin Sponge Support.., Lin, K.H; Maeda, S; Saito, T, 1995, 21, 19, 27
• Refereed, Journal of Biochemistry, DNA Fragmentation Induced in High-Cell-Density Culture of Primary Rat Hepatocytes Is an Active Process Dependent on Energy Availability, Gene Expression, and Calmodulin., MAEDA Sumio; Maeda, S; Suzuki, A; Lin, K.H; Inagaki, H; Saito, T, 1995, 118, 1161, 1165
• Refereed, Bioscience, Biotechnology, and Biochemistry, Inhibition by Retinoic Acid of Albumin and DNA Synthesis in Adult Rat Hepatocytes., Lin, K.H; Maeda, S; Koga, N; Saito, T, 1994, 58, 584, 585, 10.1271/bbb.58.584

  DOI URL
• Refereed, Applied and Microbiological Biotechnology, Immobilization and Characterization of a Thermostable ß-Galactosidase from a Thermophilic Anaerobe on a Porous Ceramic Support, Saito, T; Yoshida, Y; Kawashima, K; Lin, K.H; Maeda, S; Kobayashi, T, 1994, 40, 618, 621, 10.1007/s002530050038

  DOI URL
• Not Refereed, 工業技術, プログラムされた細胞死：アポトーシス（工業技術）, 前田 純夫, 1993, 34, 6, 23, 24
• Not Refereed, 通産ジャーナル, プログラムされた細胞死 : アポトーシス （通産ジャーナル）, 前田 純夫, 1993
• Refereed, Biochemical and Biophysical Research Communications, Cell Density-Dependent DNA Fragmentation and Its Suppression by Heparin in Primary Culture of Adult Rat Hepatocytes., MAEDA Sumio; Maeda, S; Kimura, H; Koga, N; Lin, K.H; Saito, T, 1993, 195, 270, 275, 10.1006/bbrc.1993.2040

  DOI URL
• Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, A MODIFIED COLORIMETRIC MTT ASSAY ADAPTED FOR PRIMARY CULTURED-HEPATOCYTES - APPLICATION TO PROLIFERATION AND CYTOTOXICITY ASSAYS, M OKA; S MAEDA; N KOGA; K KATO; T SAITO, Sep. 1992, 56, 9, 1472, 1473, 10.1271/bbb.56.1472

  DOI URL
• Refereed, JOURNAL OF FERMENTATION AND BIOENGINEERING, SOC FERMENTATION BIOENGINEERING, JAPAN, OVERPRODUCTION OF THERMOSTABLE BETA-GALACTOSIDASE IN ESCHERICHIA-COLI, ITS PURIFICATION AND MOLECULAR-STRUCTURE, T SAITO; K KATO; S MAEDA; T SUZUKI; S SHIBA; S IIJIMA; T KOBAYASHI, The lacN gene encoding thermostable, beta-galactosidase from a thermophilic anaerobe strain NA10 was overexpressed in Escherichia coli strain MV1184 by cloning the gene downstream from the lac promoter on pUC119. The amount of the enzyme produced by the E. coli transformant was estimated to be about 20% of the total cellular proteins when induced with isopropyl-beta-D-thiogalactopyranoside. Fed-batch culture of this strain resulted in about a 2,400-fold increase in enzyme production as compared to that in the original bacterium, strain NA10. The enzyme was purified, and its molecular structure was determined by SDS-polyacrylamide gel electrophoresis and gel filtration to be a tetrametric protein consisting of identical subunits., 1992, 74, 1, 12, 16, Scientific journal, 10.1016/0922-338X(92)90260-2

  DOI URL
• Refereed, FEMS Microbiology Letters, Expression of micF Involved in Porin Synthesis in Escherichia coli: Two Distinct Cis-Acting Elements Respectively Regulate micF Expression Positively and Negatively, MAEDA Sumio; Takayanagi, K; Maeda, S; Mizuno, T, 1991, 83, 39, 44, 10.1016/0378-1097(91)90440-L

  DOI URL
• Refereed, Journal of Biochemistry, Activation of the Osmoregulated ompC Gene by the OmpR Protein in Escherichia coli : A Study Involving Synthetic OmpR-Binding Sequences, MAEDA Sumio; Maeda, S; Takayanagi, K; Nishimura, Y; Maruyama, T; Mizuno, T, 1991, 3, 324, 327
• Refereed, JOURNAL OF BACTERIOLOGY, AMER SOC MICROBIOLOGY, EVIDENCE FOR MULTIPLE OMPR-BINDING SITES IN THE UPSTREAM ACTIVATION SEQUENCE OF THE OMPC PROMOTER IN ESCHERICHIA-COLI - A SINGLE OMPR-BINDING SITE IS CAPABLE OF ACTIVATING THE PROMOTER, S MAEDA; T MIZUNO, Jan. 1990, 172, 1, 501, 503, Scientific journal
• Refereed, Journal of Biological Chemistry, Activation of the ompC Gene by the OmpR Protein in Escherichia coli : the Cis-Acting Upstream Sequence Can Function in Both Orientations with Respect to the Canonical Promoter., MAEDA Sumio; Maeda; S. Mizuno, T, 1988, 263, 14629, 14633
• Refereed, Journal of Molecular Biology, Stereospecific Positioning of the Canonical Promoter Is Required for Activation of the ompC Gene by a Positive Regulator, OmpR, in Escherichia coli., MAEDA Sumio; Maeda, S; Ozawa, Y; Mizuno, T; Mizushima, S, 1988, 202, 433, 441, 10.1016/0022-2836(88)90276-8

  DOI URL
• Refereed, Microorganisms, MDPI AG, Characterization of Escherichia coli Persisters from Biofilm Culture: Multiple Dormancy Levels and Multigenerational Memory in Formation, Hirona Ikeda; Sumio Maeda, Chosen as "Feature Paper" Persister cells (PCs), a subpopulation occurring within normal cells, exhibit a transient tolerance to antibiotics because of their dormant state. PCs are categorized into two types: type I PCs, which emerge during the stationary phase, and type II PCs, which emerge during the logarithmic phase. Using the conventional colony-forming method, we previously demonstrated that type I PCs of Escherichia coli form more frequently in air–solid biofilm culture than in liquid culture. In the current study, we modified a cell filamentation method as a more efficient and rapid alternative for quantifying PCs. This modified method yielded results consistent with those of the conventional method with 103–104 times higher sensitivity and less detection time, within several hours, and further revealed the existence of multiple levels of type I PCs, including a substantial number of deeply dormant cells. This study also discovered a potential epigenetic memory mechanism, spanning several generations (four or six cell divisions), which influences type II PC formation based on prior biofilm experience in E. coli., 13 Sep. 2024, 12, 9, 1888, 1888, Scientific journal, 10.3390/microorganisms12091888

  PermalinkDOI URL共同研究・競争的資金等URL
• Refereed, Biochemical and Biophysical Research Communications, Elsevier BV, Escherichia coli persisters in biofilm can perform horizontal gene transfer by transformation, Tsubasa Nasu; Sumio Maeda, Open Access, Available online 13 August 2024 Persisters represent a subset of cells that exhibit transient tolerance to antimicrobials. These persisters can withstand sudden exposure to antimicrobials, even as the majority of normal cells perish. In this study, we have demonstrated the capacity of ampicillin-tolerant and alkali-tolerant persisters to execute horizontal gene transfer via in situ transformation within biofilms. Air–solid biofilms, comprising two Escherichia coli populations each with a distinct plasmid, were formed on agar media. They were treated with lethal doses of ampicillin or NaOH for 24 h, followed by a 1-min glass-ball roll. This process led to a high frequency of horizontal plasmid transfer (10−7–10−6 per cell) from dead cells to surviving persisters within the biofilms. Plasmid transfer was DNase-sensitive and also occurred by adding purified plasmid DNA to plasmid-free biofilms, demonstrating a transformation mechanism. This marks the first evidence of persisters’ novel ability for horizontal gene transfer, via transformation., Aug. 2024, 738, 150549, 150549, Scientific journal, True, 10.1016/j.bbrc.2024.150549

  PermalinkDOI URL共同研究・競争的資金等URL

MISC

• Not Refereed, 2020, 2020, 14614, https://doi.org/10.1101/2020.06.09.141614

Books etc

• Methods in Molecular Biology "Horizontal Gene Transfer" 2075 179-187, Chapter 13: Natural Transformation in Escherichia coli, Springer Nature, Komiyama Y; Maeda S, 2020
• 食物科学概論 改訂版, 朝倉書店, 6.3 バイオテクノロジーと食物, 2014, vii, 165p, 9784254606263

  CiNii Books URL
• 食物科学概論、6.3 バイオテクノロジーと食物, 朝倉書店, 2003, Not Refereed
• The Hepatocyte Review, Chapter 18: Mechanisms of Active Cell Death in Isolated Hepatocytes, Kluwer Academic Publishers, Maeda S, 2000, Not Refereed
• 食品衛生学 第９版, 第一出版, 植木幸英; 前田純夫; 阿部尚樹, Mar. 2023, 9784804114668
• 9784804114668

Presentations

• 笠垣さゆり; 小宮山瑶子; 漆谷智加; 前田純夫, 第43回日本分子生物学会年会, 大腸菌のDNAメチル化遺伝子変異のpersister cell記憶保持機構への影響, Dec. 2020

  共同研究・競争的資金等URL
• 漆谷智加; 鈴木江梨果; 前田純夫, 第43回日本分子生物学会年会, ウシ血清添加がEscherichia coliのpersister cell形成に及ぼす促進および記憶効果, Dec. 2020

  共同研究・競争的資金等URL
• Sayuri Kasagaki; Yoko Komiyama; Tomoka Urushidani; Sumio Maeda, FEMS ONLINE CONFERENCE ON MICROBIOLOGY 2020, Involvement of DNA Methylation in Memory Phenomenon of Persisters in Escherichia coli, Oct. 2020

  共同研究・競争的資金等URL
• Tomoka Urushidani; Yoko Komiyama; Sumio Maeda, FEMS ONLINE CONFERENCE ON MICROBIOLOGY 2020, Promoting and memory effects of air-solid biofilm culture on persister formation : analysis on mutants of toxin-antitoxin system genes, Oct. 2020

  共同研究・競争的資金等URL
• Sayuri Kasagaki; Mayuko Hashimoto; Haruna Akase; Yuna Hayase; Sumio Maeda, World Microbe Forum, Sub-minimal inhibitory concentration (sub-MIC) of ampicillin promotes horizontal plasmid transfer by transformation during culture in Escherichia coli, Poster presentation, 22 Jul. 2021, 20 Jul. 2021, 24 Jul. 2021
• 早瀬裕菜; 笠垣さゆり; 前田純夫, 第95回日本細菌学会総会, 最小増殖阻止濃度のタンパク質合成阻害系抗生物質と機械的刺激は大腸菌の細胞間形質転換を相乗的に促進する, Poster presentation, Mar. 2022, Mar. 2022, Mar. 2022
• 笠垣さゆり; 橋本茉由子; 前田純夫, 第44回日本分子生物学会年会, 最小増殖濃度以下のアンピシリンと機械的刺激は相乗的に大腸菌の細胞間形質転換とプラスミドDNA形質転換を促進する, Poster presentation, 02 Dec. 2021, 01 Dec. 2021, 03 Dec. 2021
• 早瀬裕菜; 三田村裕葉; 笠垣さゆり; 前田純夫, 第44回日本分子生物学会年会, 最少増殖阻止濃度のタンパク質合成阻害系抗生物質は大腸菌の形質転換を促進する, Poster presentation, 02 Dec. 2021, 01 Dec. 2021, 03 Dec. 2021
• 赤瀬はるな; 河村妙奈; 笠垣さゆり; 前田純夫, 第44回日本分子生物学会年会, 最少増殖阻止濃度のペプチドグリカン合成阻害系抗生物質は大腸菌の形質転換を促進する, Poster presentation, 02 Dec. 2021, 01 Dec. 2021, 03 Dec. 2021
• 漆谷智加; 坂本七海; 渡邊舞; 前田純夫, 第44回日本分子生物学会年会, 気相-固相バイオフィルム培養による大腸菌のパーシスター形成促進とその長期維持に関与する遺伝子の探索, Poster presentation, 01 Dec. 2021, 01 Dec. 2021, 03 Dec. 2021

  共同研究・競争的資金等URL
• 前田純夫, 第9回産学連携事例発表会（一般社団法人 食品微生物科学協会）, バイオフィルムの中で起こること, Invited oral presentation, 10 Mar. 2023, 10 Mar. 2023, 10 Mar. 2023

  共同研究・競争的資金等URL
• 赤瀬はるな; 笠垣さゆり; 前田純夫, 第45回日本分子生物学会年会, 最小増殖阻止濃度以下の細胞壁合成阻害系抗生物質と機械的刺激は大腸菌の細胞間形質転換を協調的に促進する, Poster presentation, 02 Dec. 2022, 30 Nov. 2022, 02 Dec. 2022

  共同研究・競争的資金等URL
• 早瀬裕菜; 笠垣さゆり; 前田純夫, 第45回日本分子生物学会年会, 最小増殖阻止濃度以下のタンパク質合成阻害系抗生物質と機械的刺激は大腸菌の細胞間形質転換を協調的に促進する, Poster presentation, 02 Dec. 2022, 30 Nov. 2022, 02 Dec. 2022

  共同研究・競争的資金等URL
• 那須羽; 池田寛菜; 前田純夫, 第45回日本分子生物学会年会, 大腸菌における細胞溶解液耐性細胞の形成：培養様式が及ぼす影響, Poster presentation, 30 Nov. 2022, 30 Nov. 2022, 02 Dec. 2022

  共同研究・競争的資金等URL
• 池田寛菜; 那須羽; 前田純夫, 第45回日本分子生物学会年会, 大腸菌の液体培養と気相-固相バイオフィルム培養におけるパーシスター細胞形成の解析：細胞伸長法の適用, Poster presentation, 30 Nov. 2022, 30 Nov. 2022, 02 Dec. 2022

  共同研究・競争的資金等URL
• Akase H; Kawamura T; Kasagaki S; Maeda S, International Union of Microbiological Societies (IUMS) 2022, SUB-MINIMAL INHIBITORY CONCENTRATIONS OF ANTIBIOTICS THAT INHIBIT PEPTIDOGLYCAN SYNTHESIS PROMOTE PLASMID TRANSFORMATION IN ESCHERICHIA COLI, Poster presentation, 20 Jul. 2022, 20 Jul. 2022, 22 Jul. 2022

  共同研究・競争的資金等URL
• Hayase Y; Mitamura H; Kasagaki S; Maeda S, International Union of Microbiological Societies (IUMS) 2022, SUB-MINIMAL INHIBITORY CONCENTRATIONS OF ANTIBIOTICS THAT INHIBIT PROTEIN-SYNTHESIS PROMOTE PLASMID TRANSFORMATION IN ESCHERICHIA COLI, Poster presentation, 20 Jul. 2022, 20 Jul. 2022, 22 Jul. 2022

  共同研究・競争的資金等URL
• 漆谷智加; 小宮山瑶子; 鈴木江梨果; 宮上沙貴; 近藤優; 森川美貴; 前田純夫, 第93回日本細菌学会総会, 細菌バイオフォルムにおけるpersister cell形成の解析：その記憶現象および変異株解析, Poster presentation, Feb. 2020
• 橋本茉由子; 長谷川晴香; 前田純夫, 第93回日本細菌学会総会, 大腸菌の固相－気相バイフィルム中でのcell-to-cell transformationへの温度の影響解析, Poster presentation, Feb. 2020
• 望月美奈子; 植田まりや; 中岡栞; 中谷美幸; 前田 純夫, 第93回日本細菌学会総会, 市販香辛料に付着するカルバペネーゼ遺伝子の分布と解析および全長遺伝子の単離, Poster presentation, Feb. 2020
• Hashimoto M; Hasegawa H; Maeda S, FEMS Congress 2019, High temperature promotes cell-to-cell plasmid transformation in solid-air biofilms of Escherichia coli, Poster presentation, Jul. 2019
• Mochizuki M; Nakatani M; Ueda M; Nakaoka S; Maeda S, FEMS Congress 2019, Detection and analysis of carbapenemase genes on the surface of commercially- available spices, Poster presentation, Jul. 2019
• 橋本茉由子; 前田純夫, 第41回日本分子生物学会年会, 大腸菌の固相―気相バイオフィルム中での非接合性・非ウイ ルス性細胞間プラスミド転移現象への温度の影響解析, Poster presentation, Dec. 2018
• 望月美奈子; 中谷美幸; 前田純夫, 第41回日本分子生物学会年会, 市販香辛料に付着する抗生物質耐性遺伝子の分布と解析, Poster presentation, Dec. 2018
• Yoko Komiyama; Erika Suzuki; Saki Miyaue; Menglu Su; Nozomi Kan; Sumio Maeda, FoodMicro 2018 (26th International ICFMH Conference), Promoting effect of air-solid bio lm culture on persister cell formation in Escherichia coli, Poster presentation, Oct. 2018
• 工藤さやか; 佐藤文佳; 前田純夫, 第91回日本生化学会大会, 大腸菌ECOR株の形質によるプロファイリング, Poster presentation, Sep. 2018
• Erika Suzuki; Sumio Maeda, 日本微生物生態学会第3 2 回大会, ウシ血清添加がEscherichia coli のpersister cell形成に及ぼす影響, Poster presentation, Aug. 2018
• 平山絢音; 赤瀬はるな; 早瀬裕菜; 木村優花; 前田純夫, 第46回日本分子生物学会年会, 最小増殖阻止濃度以下の抗生物質と機械的刺激により協調的に促進される大腸菌細胞間形質転換の 機構解析（ポスター）, Poster presentation, 07 Dec. 2023, 06 Dec. 2023, 08 Dec. 2023

  共同研究・競争的資金等URL
• 那須羽; 池田寛菜; 今岡南名子; 木下莉子; 前田純夫, 第46回日本分子生物学会年会, 酸‧アルカリ刺激と機械的刺激が大腸菌の細胞間形質転換に及ぼす協調的促進効果（ポスター）, Poster presentation, 07 Dec. 2023, 06 Dec. 2023, 08 Dec. 2023

  共同研究・競争的資金等URL
• 池田寛菜; 那須羽; 前田純夫, 第46回日本分子生物学会年会, 細胞伸⻑法を用いた大腸菌type IIパーシスター細胞形成の解析: 液体培養由来細胞と気相-固相 バイオフィルム培養由来細胞の比較（ポスター）, Poster presentation, 07 Dec. 2023, 06 Dec. 2023, 08 Dec. 2023

  共同研究・競争的資金等URL
• 池田寛菜; 那須羽; 前田純夫, 第46回日本分子生物学会年会, 細胞伸⻑法を用いた大腸菌type IIパーシスター細胞形成の解析: 液体培養由来細胞と気相-固相 バイオフィルム培養由来細胞の比較（サイエンスピッチ）, Oral presentation, 07 Dec. 2023, 06 Dec. 2023, 08 Dec. 2023

  共同研究・競争的資金等URL
• 平山絢音; 赤瀬はるな; 早瀬裕菜; 木村優花; 前田純夫, 第46回日本分子生物学会年会, 最小増殖阻止濃度以下の抗生物質と機械的刺激により協調的に促進される大腸菌細胞間形質転換の 機構解析（サイエンスピッチ）, Oral presentation, 06 Dec. 2023, 06 Dec. 2023, 08 Dec. 2023

  共同研究・競争的資金等URL
• 那須羽; 池田寛菜; 今岡南名子; 木下莉子; 前田 純夫, 第46回日本分子生物学会年会, 酸‧アルカリ刺激と機械的刺激が大腸菌の細胞間形質転換に及ぼす協調的促進効果（サイエンスピッチ）, Oral presentation, 06 Dec. 2023, 06 Dec. 2023, 08 Dec. 2023

  共同研究・競争的資金等URL
• 那須羽; 池田寛菜; 前田純夫, 日本微生物生態学会第36回大会, 化学性致死的刺激に耐性のパーシスター様細胞の解析 ―液体培養由来細胞とバイオフィルム由来細胞の比較, Poster presentation, 28 Nov. 2023, 28 Nov. 2023, 30 Nov. 2023

  共同研究・競争的資金等URL
• Tsubasa Nasu; Hirona Ikeda; Sumio Maeda, FEMS 2023 (The 10th Congress of European Microbiologists), Promoted occurrence of lysis solution-tolerant cells in an air-solid biofilm of Escherichia coli and their memory effect, Poster presentation, 12 Jul. 2023, 09 Jul. 2023, 13 Jul. 2023

  共同研究・競争的資金等URL

Awards

• Japan society, Feb. 2020

Industrial Property Rights

• Patent right, 初代肝細胞の単層シート構造体とその形成方法, 3435458
• Patent right, Continuous cell-sheet structure consisting of monolayer of primary hepatocytes and the method of forming the sheet

Research Projects

• Apr. 2022, Mar. 2024, Principal investigator, 極低濃度の抗生物質が示す新作用:細菌の細胞間形質転換を促進する作用, 前田純夫, 公益財団法人発酵研究所, Competitive research funding, rm:published_papers;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations

  対象リソースIRI
• Apr. 2022, Mar. 2024, Principal investigator, 極低濃度の抗生物質が示す新作用:細菌の細胞間形質転換を促進する作用, 前田純夫, 公益財団法人発酵研究所, Competitive research funding, rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations

  対象リソースIRI
• Grant-in-Aid for Scientific Research (C), Apr. 2019, Mar. 2025, 19K05791, Principal investigator, Elucidation of the "memory" phenomenon of bacterial persister formation, 前田 純夫, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Nara Women's University, 4420000, 3400000, 1020000, rm:published_papers;rm:published_papers;rm:published_papers;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations

  対象リソースIRI
• Apr. 2022, Mar. 2024, Principal investigator
• Apr. 2019, Mar. 2023, 19K05791, Principal investigator
• Grant-in-Aid for Scientific Research (C), Apr. 2019, Mar. 2023, 19K05791, Principal investigator, 細菌のパーシスター細胞化の『記憶』現象の機構解明, 前田 純夫, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Nara Women's University, 4420000, 3400000, 1020000, rm:published_papers;rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations

  対象リソースIRI
• Grant-in-Aid for Challenging Exploratory Research, Apr. 2015, Mar. 2018, 15K14694, Principal investigator, Analysis on meromy phenomenon in bacterial cells: based on the finding of memory phenomenon in high persistence expression, Maeda Sumio, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Nara Women's University, 4160000, 3200000, 960000, Persister cells are a specific subpopulation of bacterial cells that have acquired temporary antibiotic-resistant phenotypes. In this study, we showed that Escherichia coli produces many more persister cells in colony-biofilm culture than in the usual liquid culture and that these persisters can be maintained in higher numbers than those from liquid culture for up to 4 weeks at 37 °C in a fresh, nutrient-rich, antibiotic-containing medium, even after complete withdrawal from the colony-biofilm culture. This suggests the presence of a long-retention effect, or “memory effect”, in the persister cell state of E. coli cells. We also discovered that such increases in persisters during colony-biofilm culture and their memory effects are common not only within E. coli species but also in other bacterial species. In addition, using single-gene knock-out mutants of E. coli, we identified several candidate genes involved in this phenomenon., rm:published_papers;rm:presentations

  対象リソースIRI
• Apr. 2015, Mar. 2018, 15K14694, Principal investigator
• Grant-in-Aid for Scientific Research (B), Apr. 2013, Mar. 2017, 25292051, Principal investigator, Study on mechanisms and generality of a new type of horizontal gene transfer in Escherichia coli, MAEDA SUMIO; Shibata Yuka; Matsumoto Akiko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Nara Women's University, 17290000, 13300000, 3990000, This study aimed at revealing the mechanisms and the generality of a new type of horizontal gene transfer in Escherichia coli, which we termed “cell-to-cell transformation”. We found that 1) some natural E. coli strains can undergo DNase-sensitive cell-to-cell transformation, 2) cell-to-cell transformation between some natural E. coli strains occurs in a phage-independent manner, and that 3) frequent cell-to-cell transformation involving a specific laboratory strain occurs via the function of a certain phage and its related protein(s) mainly by a DNase-sensitive transformation mechanism. By these results, we have achieved an elucidation of the mechanisms of cell-to-cell transformation and a demonstration of the generality of cell-to-cell transformation within the E. coli species., rm:published_papers;rm:published_papers;rm:published_papers;rm:published_papers;rm:presentations

  対象リソースIRI
• Grant-in-Aid for Challenging Exploratory Research, Apr. 2015, Mar. 2018, 15K14694, Principal investigator, Analysis on meromy phenomenon in bacterial cells: based on the finding of memory phenomenon in high persistence expression, Maeda Sumio, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Nara Women's University, 4160000, 3200000, 960000, Persister cells are a specific subpopulation of bacterial cells that have acquired temporary antibiotic-resistant phenotypes. In this study, we showed that Escherichia coli produces many more persister cells in colony-biofilm culture than in the usual liquid culture and that these persisters can be maintained in higher numbers than those from liquid culture for up to 4 weeks at 37 °C in a fresh, nutrient-rich, antibiotic-containing medium, even after complete withdrawal from the colony-biofilm culture. This suggests the presence of a long-retention effect, or “memory effect”, in the persister cell state of E. coli cells. We also discovered that such increases in persisters during colony-biofilm culture and their memory effects are common not only within E. coli species but also in other bacterial species. In addition, using single-gene knock-out mutants of E. coli, we identified several candidate genes involved in this phenomenon., rm:published_papers

  対象リソースIRI
• Apr. 2013, Mar. 2017, 25292051, Principal investigator
• Apr. 2013, Mar. 2017, 25292051, Principal investigator
• Grant-in-Aid for Challenging Exploratory Research, Apr. 2012, Mar. 2015, 24650488, Principal investigator, Trials for gene transduction with bacteriophages to aim at making enteric bacteria into probiotics, MAEDA SUMIO, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Nara Women's University, 3900000, 3000000, 900000, In this study, in order to establish a method of making in vivo enteric bacteria “probiotics”, we aimed to develop a basic method of gene transfer to enteric bacteria with bacteriophages. We examined various experimental conditions to introduce genes into enteric bacteria with a broad host-range phage. We found that gene transfer was possible using the phage on some wild strains of Escherichia coli or some coliforms isolated from the intestine or the cecum of rat. Further examinations to achive more efficient and broader introduction are required in order to apply this method as a practical one for in vivo gene introduction., rm:published_papers

  対象リソースIRI
• Grant-in-Aid for Scientific Research (B), Apr. 2013, Mar. 2017, 25292051, Principal investigator, Study on mechanisms and generality of a new type of horizontal gene transfer in Escherichia coli, MAEDA SUMIO; Shibata Yuka; Matsumoto Akiko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Nara Women's University, 17290000, 13300000, 3990000, This study aimed at revealing the mechanisms and the generality of a new type of horizontal gene transfer in Escherichia coli, which we termed “cell-to-cell transformation”. We found that 1) some natural E. coli strains can undergo DNase-sensitive cell-to-cell transformation, 2) cell-to-cell transformation between some natural E. coli strains occurs in a phage-independent manner, and that 3) frequent cell-to-cell transformation involving a specific laboratory strain occurs via the function of a certain phage and its related protein(s) mainly by a DNase-sensitive transformation mechanism. By these results, we have achieved an elucidation of the mechanisms of cell-to-cell transformation and a demonstration of the generality of cell-to-cell transformation within the E. coli species., rm:published_papers;rm:published_papers;rm:published_papers;rm:published_papers

  対象リソースIRI
• Grant-in-Aid for Challenging Exploratory Research, Apr. 2012, Mar. 2015, 24650488, Principal investigator, Trials for gene transduction with bacteriophages to aim at making enteric bacteria into probiotics, MAEDA SUMIO, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Nara Women's University, 3900000, 3000000, 900000, In this study, in order to establish a method of making in vivo enteric bacteria “probiotics”, we aimed to develop a basic method of gene transfer to enteric bacteria with bacteriophages. We examined various experimental conditions to introduce genes into enteric bacteria with a broad host-range phage. We found that gene transfer was possible using the phage on some wild strains of Escherichia coli or some coliforms isolated from the intestine or the cecum of rat. Further examinations to achive more efficient and broader introduction are required in order to apply this method as a practical one for in vivo gene introduction., rm:published_papers

  対象リソースIRI
• Grant-in-Aid for Scientific Research (C), 2007, 2009, 19580089, Principal investigator, Study on a novel phenomenon of horizontal gene transfer in bacterial colony biofilms, MAEDA Sumio, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Nara Women's University, 4810000, 3700000, 1110000, This study aimed at revealing the detail and the generality of nonconjugative, nonviral horizontal gene transfer in colony biofilms of Escherichia coli. We found that this horizontal gene transfer is a novel kind of transformation, that there are several dozens of genes which are involved in this gene transfer in E.coli, and that this gene transfer occurs on the culture on food-based media as well as between E.coli and Bacillus subtilis., rm:published_papers;rm:published_papers;rm:published_papers;rm:published_papers;rm:published_papers;rm:published_papers

  対象リソースIRI
• 2001, バイオフィルム中での細菌細胞の生理, 0, 0, 0, Competitive research funding
• 2001, 細菌間遺伝子水平伝播, 0, 0, 0, Competitive research funding
• バイオフィルム制御, 0, 0, 0, Competitive research funding
• 食環境中での細菌の挙動, 0, 0, 0, Competitive research funding
• Apr. 2019, Mar. 2024, 19K05791, Principal investigator
• Apr. 2019, Mar. 2024, 19K05791, Principal investigator