Researchers Database

Mannari Takayo

    Faculty Division of Human Life and Environmental Sciences Research Group of Food Science and Nutrition Assistant Professor
Last Updated :2021/06/02

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Research Areas

  • Life sciences, Nutrition and health science
  • Life sciences, Neuroscience - general
  • Humanities & social sciences, Home economics, lifestyle science

Research Experience

  • Apr. 2017, Nara Women's University, 生活環境科学系 食物栄養学領域, 助教
  • Apr. 2014 Mar. - 2017, 日本学術振興会特別研究員(DC1)
  • Apr. 2011 Mar. - 2012, Nara Medical University, 女性研究者支援センター, 研究支援員

Education

  • Apr. 2011, Mar. - 2017, Nara Medical University, Graduate School of Medical Science Course
  • Apr. 2007, Mar. - 2011, 畿央大学, Faculty of health science, Department of Health and Nutrition

Published Papers

  • Bone marrow stromal cell sheets may promote axonal regeneration and functional recovery with suppression of glial scar formation after spinal cord transection injury in rats

    Akinori Okuda; Noriko Horii-Hayashi; Takayo Sasagawa; Takamasa Shimizu; Hideki Shigematsu; Eiichiro Iwata; Yasuhiko Morimoto; Keisuke Masuda; Munehisa Koizumi; Manabu Akahane; Mayumi Nishi; Yasuhito Tanaka

    OBJECTIVE Transplantation of bone marrow stromal cells (BMSCs) is a theoretical potential as a therapeutic strategy in the treatment of spinal cord injury (SCI). Although a scaffold is sometimes used for retaining transplanted cells in damaged tissue, it is also known to induce redundant immunoreactions during the degradation processes. In this study, the authors prepared cell sheets made of BMSCs, which are transplantable without a scaffold, and investigated their effects on axonal regeneration, glial scar formation, and functional recovery in a completely transected SCI model in rats. METHODS BMSC sheets were prepared from the bone marrow of female Fischer 344 rats using ascorbic acid and were cryopreserved until the day of transplantation. A gelatin sponge (GS), as a control, or BMSC sheet was transplanted into a 2-mm-sized defect of the spinal cord at the T-8 level. Axonal regeneration and glial scar formation were assessed 2 and 8 weeks after transplantation by immunohistochemical analyses using anti-Tuj1 and glial fibrillary acidic protein (GFAP) antibodies, respectively. Locomotor function was evaluated using the Basso, Beattie, and Bresnahan scale. RESULTS The BMSC sheets promoted axonal regeneration at 2 weeks after transplantation, but there was no significant difference in the number of Tuj1-positive axons between the sheet-and GS-transplanted groups. At 8 weeks after transplantation, Tuj1-positive axons elongated across the sheet, and their numbers were significantly greater in the sheet group than in the GS group. The areas of GFAP-positive glial scars in the sheet group were significantly reduced compared with those of the GS group at both time points. Finally, hindlimb locomotor function was ameliorated in the sheet group at 4 and 8 weeks after transplantation. CONCLUSIONS The results of the present study indicate that an ascorbic acid induced BMSC sheet is effective in the treatment of SCI and enables autologous transplantation without requiring a scaffold., AMER ASSOC NEUROLOGICAL SURGEONS, Mar. 2017, JOURNAL OF NEUROSURGERY-SPINE, 26 (3), 388 - 395, doi;web_of_science

    Scientific journal

  • Long-term effects of maternal separation coupled with social isolation on reward seeking and changes in dopamine D1 receptor expression in the nucleus accumbens via DNA methylation in mice

    Takayo Sasagawa; Noriko Horii-Hayashi; Akinori Okuda; Takashi Hashimoto; Cho Azuma; Mayumi Nishi

    Early-life stress has long-lasting effects on the stress response, emotions, and behavior throughout an individual's life. Clinical reports have demonstrated that child abuse victims exhibit impairments in reward-associated behavior; yet, the mechanism for this effect remains unclear. Maternal separation (MS) or MS coupled with social isolation (SI) (MS + SI) is widely used as a model for early-life stress in rodent studies. We employed mice subjected to MS + SI to clarify the long-term effect of early-life stress on reward-seeking involving palatable foods by a conditioned place-preference (CPP) paradigm. Prior MS + SI experience decreased exploration time in a chocolate-paired compartment in adult female mice, but not in male mice. We then focused on the mesolimbic dopamine pathway associated with reward seeking behavior and measured both mRNA and protein levels of tyrosine hydroxylase (TH) in the ventral tegmental area (VTA) and dopamine D1 and D2 receptors in the nucleus accumbens (NAc). MS + SI female mice had significantly lower D1 receptor mRNA and protein levels than controls, whereas the expression of TH and the D2 receptor was similar in the 2 groups. All mRNA and protein levels were unchanged in MS + SI male mice. When attempting to elucidate the mechanism underlying downregulation of the Dl receptor in the NAc of MS + SI females, we found hypermethylation of the Drd1a promoter region. These results suggest that early-life stress affects reward-seeking behavior in female mice, which may be associated with the downregulation of D1 receptor in the NAc via epigenetic modification of its promoter region. (C) 2017 Elsevier B.V. All rights reserved., ELSEVIER IRELAND LTD, Feb. 2017, NEUROSCIENCE LETTERS, 641, 33 - 39, doi;web_of_science

    Scientific journal

  • Effects of early life adverse experiences on the brain: implications from maternal separation

    Mayumi Nishi; Takayo Sasagawa; Noriko Horii

    2017, Folia Pharmacologica Japonica, 149 (2), 72 - 75

    Scientific journal

  • Insights from extracellular matrix studies in the hypothalamus: structural variations of perineuronal nets and discovering a new perifornical area of the anterior hypothalamus

    Noriko Horii-Hayashi; Takayo Sasagawa; Mayumi Nishi

    The hypothalamus controls metabolism, stress responses, and instinctive behaviors for individual survival and species preservation. Recent studies suggest that hypothalamic neurons retain plasticity throughout adulthood, which enables these neurons to respond to various kinds of changes in environment, nutrients, and fluctuating hormones. One of the mechanisms underlying the regulation of neural plasticity is the formation of a stable extracellular matrix (ECM) structure called perineuronal nets (PNNs). PNNs are large aggregates of heterogeneous ECM molecules such as chondroitin sulfate proteoglycans (CSPGs), hyaluronan, their link proteins, and tenascin-R. PNNs surround the cell body and proximal dendrites of a subset of neurons and limit adult neural plasticity. This review describes the CSPG-based ECM, including the PNNs, with a special focus on the hypothalamus of mice. We first provide an overview of PNNs in terms of their structure, molecular components, and functions, most of which have been demonstrated by extrahypothalamic studies. Second, we show the presence or absence of PNNs within individual hypothalamic regions and then describe non-PNN-formed ECM containing CSPGs that can be observed in particular hypothalamic regions. Finally, we will introduce a newly identified mouse hypothalamic area that we named the perifornical area of the anterior hypothalamus (PeFAH), which contains a cluster of PNN-positive neurons. PeFAH neurons express enkephalin and have bidirectional connections with the lateral septum. The anterior hypothalamus and lateral septum are thought to regulate defensive behaviors therefore, the PeFAH neurons and PNNs around them could be involved in the regulation of defensive behaviors., Springer Tokyo, 01 Jan. 2017, Anatomical Science International, 92 (1), 18 - 24, doi;pubmed

  • マウス脳に新たに同定した前視床下部脳弓周囲野PeFAHと情動神経回路の関わり

    堀井 謹子; 笹川 誉世; 西 真弓

    2016, BIO Clinica, 31 (3), 76 - 81

  • A newly identified mouse hypothalamic area having bidirectional neural connections with the lateral septum: the perifornical area of the anterior hypothalamus rich in chondroitin sulfate proteoglycans

    Noriko Horii-Hayashi; Takayo Sasagawa; Takashi Hashimoto; Takeshi Kaneko; Kosei Takeuchi; Mayumi Nishi

    While previous studies and brain atlases divide the hypothalamus into many nuclei and areas, uncharacterised regions remain. Here, we report a new region in the mouse anterior hypothalamus (AH), a triangular-shaped perifornical area of the anterior hypothalamus (PeFAH) between the paraventricular hypothalamic nucleus and fornix, that abundantly expresses chondroitin sulfate proteoglycans (CSPGs). The PeFAH strongly stained with markers for chondroitin sulfate/CSPGs such as Wisteria floribunda agglutinin and antibodies against aggrecan and chondroitin 6 sulfate. Nissl-stained sections of the PeFAH clearly distinguished it as a region of comparatively low density compared to neighboring regions, the paraventricular nucleus and central division of the anterior hypothalamic area. Immunohistochemical and DNA microarray analyses suggested that PeFAH contains sparsely distributed calretinin-positive neurons and a compact cluster of enkephalinergic neurons. Neuronal tract tracing revealed that both enkephalin-and calretinin-positive neurons project to the lateral septum (LS), while the PeFAH receives input from calbindin-positive LS neurons. These results suggest bidirectional connections between the PeFAH and LS. Considering neuronal subtype and projection, part of PeFAH that includes a cluster of enkephalinergic neurons is similar to the rat perifornical nucleus and guinea pig magnocellular dorsal nucleus. Finally, we examined c-Fos expression after several types of stimuli and found that PeFAH neuronal activity was increased by psychological but not homeostatic stressors. These findings suggest that the PeFAH is a source of enkephalin peptides in the LS and indicate that bidirectional neural connections between these regions may participate in controlling responses to psychological stressors., WILEY-BLACKWELL, Sep. 2015, EUROPEAN JOURNAL OF NEUROSCIENCE, 42 (6), 2322 - 2334, doi;web_of_science

    Scientific journal

  • A new area of the mouse anterior hypothalamus involved in septohypothalamic circuit

    Noriko Horii-Hayashi; Takayo Sasagawa; Mayumi Nishi

    2015, Interdisciplinary Inforamation Sciences, 21 (3), 243-251

  • Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain

    Noriko Horii-Hayashi; Takayo Sasagawa; Wataru Matsunaga; Mayumi Nishi

    Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In adult mammals, CSPGs form the specialized ECM structure perineuronal nets (PNNs) that surround somata and dendrites of certain types of neurons. PNNs restrict synaptic plasticity and regulate the closure of critical periods. Although previous studies have examined the starting period of PNN formation, focusing on primary sensory cortices, there are no systematic studies at the whole brain level. Here, we examined the starting period of PNN formation in male mice ranging in age from postnatal day 3 to week 11, mainly focusing on several cortical areas, limbic structures, hypothalamus, and brain stem, using lectin histochemistry with Wisteria floribunda agglutinin (WFA). Results showed that early PNN formation was observed in several reticular formations of the brain stem related to the cranial nerves and primary somatosensory cortices. In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala. Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms. The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain., HINDAWI PUBLISHING CORP, 2015, NEURAL PLASTICITY, 25, 158 - 167, doi;web_of_science

    Scientific journal

  • Effects of early life adverse experiences on the brain: implications from maternal separation models in rodents

    Mayumi Nishi; Noriko Horii-Hayashi; Takayo Sasagawa

    During postnatal development, adverse early life experiences affect the formation of neuronal networks and exert long-lasting effects on neural function. Many studies have shown that daily repeated maternal separation (MS), an animal model of early life stress, can regulate the hypothalamic-pituitary-adrenal axis (HPA axis) and affect subsequent brain function and behavior during adulthood. However, the molecular basis of the long-lasting effects of early life stress on brain function has not been fully elucidated. In this mini review, we present various cases of MS in rodents and illustrate the alterations in HPA axis activity by focusing on corticosterone (CORT). We then show a characterization of the brain regions affected by various patterns of MS, including repeated MS and single time MS at various stages before weaning, by investigating c-Fos expression. These CORT and c-Fos studies suggest that repeated early life stress may affect neuronal function in region- and temporal-specific manners, indicating a critical period for habituation to early life stress. Next, we introduce how early life stress can impact behavior, namely by inducing depression, anxiety or eating disorders, and alterations in gene expression in adult mice subjected to MS., FRONTIERS RESEARCH FOUNDATION, Jun. 2014, FRONTIERS IN NEUROSCIENCE, 8 (166), doi;web_of_science

  • Developmental Changes in Desensitisation of c-Fos Expression Induced by Repeated Maternal Separation in Pre-Weaned Mice

    N. Horii-Hayashi; T. Sasagawa; W. Matsunaga; Y. Matsusue; C. Azuma; M. Nishi

    Early-life stress has long-lasting effects on neuroendocrine and behaviour in adulthood. Maternal separation (MS) is used as a model of early-life stress and daily repeated MS (RMS) for 3 h during the first two postnatal weeks is widely used in rodent studies. However, it is not fully understood whether early-life animals desensitise/habituate to repeated stress. In the present study, we investigated the effects of daily RMS for 3 h and acute/single time MS (SMS) for 3 h on the plasma corticosterone level and c-Fos expression in the brain in mice at different postnatal ages. Mice were subjected to: (i) RMS from postnatal day (PND) 1 to 14 (RMS14); (ii) RMS from PND14 to 21 (RMS21); (iii) SMS on PND14 (SMS14); and (iv) SMS on PND21 (SMS21). Plasma corticosterone and c-Fos expression were examined on the final day in each experiment. The basal corticosterone levels in RMS14 and RMS21 were equal to those in respective age-matched controls. After the final separation, the levels were significantly increased and were comparable with those after SMS14 and SMS21, respectively. Histological analysis indicated that c-Fos expression significantly increased in many brain regions, including the paraventricular nucleus, prefrontal cortex, hippocampus, and basolateral and medial amygdale in both SMS14 and SMS21 mice. However, c-Fos expression in RMS14 mice significantly increased in many regions, whereas such increases were hardly seen in RMS21 mice. These results indicate that repeated early-life stress neither increases basal corticosterone, nor decreases the magnitude of the corticosterone response during the first three postnatal weeks, although desensitisation of c-Fos expression induced by repeated stress is changed during postnatal development., WILEY-BLACKWELL, Feb. 2013, JOURNAL OF NEUROENDOCRINOLOGY, 25 (2), 158 - 167, doi;web_of_science

    Scientific journal

  • Effects of early life stress on brain activity: Implications from maternal separation model in rodents

    Mayumi Nishi; Noriko Horii-Hayashi; Takayo Sasagawa; Wataru Matsunaga

    Adverse experiences in early life can affect the formation of neuronal circuits during postnatal development and exert long-lasting influences on neural function. Many studies have shown that daily repeated maternal separation (RMS), an animal model of early life stress, can modulate the hypothalamic-pituitary-adrenal axis (HPA-axis) and can affect subsequent brain function and emotional behavior during adulthood. However, the molecular basis of the long-lasting effects of early life stress on brain function has not been completely elucidated. In this mini-review, we introduce various cases of maternal separation in rodents and illustrate the alterations in HPA-axis activity by focusing on corticosterone (CORT), an end-product of the HPA-axis in rodents. We then present the characterization of the brain regions affected by various patterns of MS, including RMS and single time maternal separation (SMS) at various stages before weaning, by investigating c-Fos expression, a biological marker of neuronal activity. These CORT and c-Fos studies suggest that repeated early life stress may affect neuronal function in region- and temporal-specific manners, indicating a critical period for habituation to early life stress. Furthermore, we introduce changes in behavioral aspects and gene expression in adult mice exposed to RMS. (C) 2012 Elsevier Inc. All rights reserved., ACADEMIC PRESS INC ELSEVIER SCIENCE, Jan. 2013, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 181, 306 - 309, doi;web_of_science

    Scientific journal

  • 幼少期のストレスが脳の可塑性に及ぼす影響

    西 真弓; 堀井‐林 謹子; 笹川 誉世; 松永 渉

    2012, 日本生理学雑誌, 74, 216 - 217

  • The effects of maternal separation on behaviours under social-housing environments in adult male C57BL/6 mice.

    Nozomi Endo; Manabu Makinodan; Takayo Mannari-Sasagawa; Noriko Horii-Hayashi; Nami Somayama; Takashi Komori; Toshifumi Kishimoto; Mayumi Nishi

    Adverse experience in early life can affect the formation of neuronal circuits during postnatal development and exert long-lasting influences on neural functions that can lead to the development of a variety of psychiatric disorders including depression, anxiety disorders, and post-traumatic stress disorder. Many studies have demonstrated that daily repeated maternal separation, an animal model of early-life stress, can induce impairments in emotional behaviours and cognitive function during adolescence and adulthood. However, the behavioural phenotypes of maternally separated mice under long-term group-housing conditions are largely unknown. In this study, we applied our newly developed assay system to investigate the effects of maternal separation on behaviours under group-housing conditions during four days of continuous observations. Using our system, we found that repeated maternal separation resulted in inappropriate social distance from cagemates, altered approach preferences to others, and induced a lower rank in the time spent on the running wheel under group-housing conditions in adult male mice. Focussing on these behavioural abnormalities that appear in an environment with a social context will be important insights to understand the pathogenesis of psychiatric disorders., 12 Jan. 2021, Scientific reports, 11 (1), 527 - 527, True, doi;pubmed;pmc

    Scientific journal

MISC

  • Effects of early life stress on brain activity : implications from maternal separation model in rodents

    Mayumi Nishi; Noriko Horii-Hayashi; Takayo Sasagawa

    SPRINGER JAPAN KK, 2013, JOURNAL OF PHYSIOLOGICAL SCIENCES, 63, S47 - S47, web_of_science

    Summary international conference

  • Activated brain regions by early-life maternal separation and its effects on construction of perineuronal nets in the limbic system

    Noriko Horii-Hayashi; Takayo Sasagawa; Yumiko Matsusue; Mayumi Nishi

    ELSEVIER IRELAND LTD, 2011, NEUROSCIENCE RESEARCH, 71, E367 - E367, doi;web_of_science

    Summary international conference

Presentations

  • イチゴの機能性および嗜好性成分の品種間差と季節変動

    岩﨑 菜都美; 久枝 由美子; 萬成 誉世; 西本 登志; 髙村 仁知

    日本家政学会関西支部 第40回(通算第 96 回)研究発表会, 24 Nov. 2018, 帝塚山大学

  • グルテンフリー米粉パン調製における製パン条件の検討 スロージューサー残渣添加の影響

    原康香; 武智多与理; 畠中芳郎; 萬成誉世; 高村仁知

    日本調理科学会平成30年度大会, 30 Aug. 2018, 30 Aug. 2018, 武庫川女子大学

  • グルテンフリー米粉パン調製における製パン条件の検討

    武智 多与理; 原 康香; 畠中 芳郎; 萬成 誉世; 髙村 仁知

    日本食品科学工学会第65回大会, Aug. 2018, 東北大学

  • 幼少期ストレスが嗜好性食餌に対する報酬行動に及ぼす影響について

    笹川 誉世; 堀井 謹子; 西 真弓

    第92回日本解剖学会近畿支部学術集会, Nov. 2016

  • 幼少期ストレスが嗜好性食餌に対する欲求行動へ与える影響について

    笹川 誉世; 堀井 謹子; 並河 知宏; 西 真弓

    第43回日本神経内分泌学会学術集会 シンポジウム「摂食とストレス」, 14 Oct. 2016, 浜松

  • 幼少期ストレスが嗜好性食餌に対する欲求行動へ与える影響について

    笹川 誉世; 堀井-林 謹子; 西 真弓

    第25回日本行動神経内分泌研究会, Sep. 2016

  • 幼少期ストレスによる食物嗜好性行動への影響について

    笹川 誉世; 堀井-林 謹子; 奥田 哲教; 西 真弓

    第39回日本神経科学大会, Jul. 2016

  • 幼少期ストレスによる嗜好性食餌に対する摂食・欲求行動への影響について

    笹川 誉世; 堀井 謹子; 奥田 哲教; 西 真弓

    第56回日本組織細胞化学会総会・学術集会, Oct. 2015

  • 幼少期ストレスによる嗜好性食餌に対する摂食・欲求行動への影響について

    笹川 誉世; 堀井 謹子; 奥田 哲教; 西 真弓

    第42回日本神経内分泌学会・第23回日本行動神経内分泌研究会 合同学術集会, Sep. 2015

  • Effects of early life stress on feeding behavior for palatable foods

    笹川 誉世; 堀井 謹子; 西 真弓

    第38回日本神経科学大会, Jul. 2015

  • Effects of early life stress on palatable feeding behavior.

    Mannari Takayo

    第37回日本神経科学大会, Sep. 2014

  • 幼少期ストレスによる食物嗜好性行動への影響

    Mannari Takayo

    第119回日本解剖学会総会?全国学術集会, Mar. 2014

  • Effects of early life stress on brain activity: implications from maternal separation in rodents.

    Mannari Takayo; Takayo Sasagawa; Noriko Horii-Hayashi; Takashi Hashimoto; Mayumi Nishi

    Neuroscience2013, Nov. 2013

  • 母子分離による脳内c-Fos発現変化と成長後の行動への影響

    Mannari Takayo

    日本行動神経内分泌研究会(JSBN) 第3回関西支部勉強会, Mar. 2013

  • Developmental changes in desensitization of c-Fos expression induced by repeated maternal separation in pre-weaned mice.

    Mannari Takayo

    第118回日本解剖学会総会・全国学術集会, Mar. 2013

  • Brain regions activated by maternal separation in the developing mouse: c-Fos expression after acute and repeated separation.

    Mannari Takayo

    第35回日本神経科学大会, Sep. 2012

  • Brain regions activated by maternal separation in the developing mouse: c-Fos expression after acute and repeated separation.

    Mannari Takayo; Takayo Sasagawa; Noriko Horii-Hayashi; Wataru Matsunaga; Yumiko Matsusue; Mayumi Nishi

    14th International Congress of Histochemistry and Cytochemistry., Aug. 2012

  • 母子分離ストレスが神経活動に及ぼす影響

    Mannari Takayo

    第87回日本解剖学会近畿支部学術集会, Dec. 2011

Association Memberships

  • 日本家政学会



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