Researchers Database

KIKUZAKI Hiroe

FacultyFaculty Division of Human Life and Environmental Sciences Research Group of Food Science and Nutrition
PositionProfessor
Last Updated :2022/10/29

researchmap

Profile and Settings

  • Name (Japanese)

    Kikuzaki
  • Name (Kana)

    Hiroe

Degree

  • Doctor of Human Life Science, Osaka City University

Research Interests

  • Food Chemistry, Natural Organic Chemistry, Cooking Science, Food Service Management, Edible plants, Spices and Herbs, Structure determination, Antioxidants, Enzyme inhibitor, Antiallergic activity, Nutraceuticals

Research Areas

  • Humanities & social sciences, Home economics, lifestyle science
  • Life sciences, Bioorganic chemistry
  • Life sciences, Food sciences

Research Experience

  • Apr. 2010, Department of Food Science & Nutrition, Nara Women's University
  • Apr. 2003, Mar. 2010, Graduate School of Human Life Science, Osaka City University・Associate Professor
  • Apr. 1999, Mar. 2003, A lecturer in Faculty of Human Life Science, Osaka City University
  • Oct. 1996, Mar. 1999, An assistant in Faculty of Human Life Science, Osaka City University
  • Oct. 1984, Sep. 1996, A technical employee in Faculty of Human Life Science, Osaka City University

Education

  • Apr. 1984, Sep. 1984, Osaka City University, Graduate School of Human Life Science, Division of Food and Nutrition
  • Apr. 1978, Mar. 1982, Osaka City University, Faculty of Human Life Science, Department of Food and Nutrition

Teaching Experience

  • Organic Chemistry I, Nara Women's University
  • Practical Training in Food Service Management, Nara Women's University
  • Practice of Food Service Management, Nara Women's University
  • Food Service Magagement II, Nara Women's University
  • Food Service Management I, Nara Women's University
  • 99 Apr. 2020, Postgraduate courses, Japan

Association Memberships

  • 日本農芸化学会
  • 日本栄養・食糧学会
  • 日本調理科学会
  • 日本家政学会
  • 日本給食経営管理学会
  • 日本栄養改善学会
  • 日本油化学会
  • 日本薬学会
  • 日本生薬学会
  • 日本フードファクター学会
  • 日本香辛料研究会
  • 植物化学研究会
  • 香料・テルペンおよび精油化学に関する討論会
  • AOU研究会
  • 抗酸化・機能研究会, Apr. 2018, 9999

Ⅱ.研究活動実績

Published Papers

  • Refereed, J. Nutr. Sci. Vitam., Effects of cooking methods on free amino acid contents in vegetables, Ito, H; Kikuzaki, H; Ueno, H, 2019, 65, 264, 271, Scientific journal
  • Refereed, Journal of Nutrition and Dietetic Practice, Free Amino Acid Compositions for Fruits, KIKUZAKI Hiroe; Hinako Ito; Hiroshi Ueno, Nov. 2017, 1, 1, 1-5
  • Refereed, International Biology Review, Novel inhibitors for histidine decarboxylase from plant components, KIKUZAKI Hiroe; Yoko Nitta; Hiroshi Ueno, Aug. 2017, 1, 2, 1-14
  • Refereed, ACTA PHYSIOLOGIAE PLANTARUM, SPRINGER HEIDELBERG, Inhibition of recombinant human histidine decarboxylase activity in different strawberry cultivars, Uno, Yuichi; Nitta, Yoko; Ishibashi, Misaki; Noguchi, Yuji; Kikuzaki, Hiroe, Strawberry fruit contains many constituents, some of which have the potential to inhibit histidine decarboxylase (HDC) activity. HDC converts L-histidine to histamine, which is associated with allergic and other biological reactions in the human body. The HDC inhibition levels were different and the component ratios varied by genotype in strawberry. Among the 11 cultivars collected locally in Japan, 'Tokun' had an approximately ten times higher inhibition ratio than the lowest cultivar. The reproducibility was confirmed using five cultivars under the same conditions in a glass greenhouse, suggesting that genotypic variation is a major factor of HDC inhibition. The potential inhibitors of HDC might be polyphenols because they showed moderate correlations with HDC inhibition rates. Among the polyphenols, the anthocyanin content possessed a moderate negative correlation. Ascorbic acid, which contributes to the overestimation of total polyphenol, did not independently inhibit HDC activity. These findings will support the identification of potential HDC inhibitors in strawberry and indicated that genotypic differences would make useful probes for inhibitor identification., Jun. 2017, 39, 6, Scientific journal
  • Refereed, 日本栄養・食糧学会大会講演要旨集, (公社)日本栄養・食糧学会, ヒトEGFR過剰発現線虫を用いたビフィズス菌のプロバイオティクス効果, 小村 智美; Shim Jaegal; 岩城 啓子; 菊崎 泰枝; 西川 禎一, Apr. 2017, 71回, 282, 282
  • Refereed, Integrative Food, Nutrition and Metabloism, Construction of a free-form amino acid database for vegetables and mushrooms, KIKUZAKI Hiroe; Hinako Ito; Hiroshi Ueno, Apr. 2017, 4, 4, 1-9
  • Refereed, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, CENTER ACADEMIC PUBL JAPAN, Strong Inhibition of Secretory Sphingomyelinase by Catechins, Particularly by (-)-Epicatechin 3-O-Gallate and (-)-3 '-O-Methylepigallocatechin 3-O-Gallate, Keiko Kobayashi; Yuki Ishizaki; Shosuke Kojo; Hiroe Kikuzaki, Sphingomyelinases (SMases) are key enzymes involved in many diseases which are caused by oxidative stress, such as atherosclerosis, diabetes mellitus, nonalcoholic fatty liver disease, and Alzheimer's disease. SMases hydrolyze sphingomyelin to generate ceramide, a well-known pro-apoptotic lipid. SMases are classified into five types based on pH optimum, subcellular localization, and cation dependence. Previously, we demonstrated that elevation of secretory sphingomyelinase (sSMase) activity increased the plasma ceramide concentration under oxidative stress induced by diabetes and atherosclerosis in murine models. These results suggest that sSMase inhibitors can prevent the progress of these diseases. The present study demonstrated that sSMase activity was activated by oxidation and inhibited by reduction. Furthermore, we examined whether catechins inhibited the sSMase activity in a physiological plasma concentration. Among catechins, (-)-epicatechin 3-O-gallate (ECg) exhibited strong inhibitory effect on sSMase (IC50=25.7 mu m). This effect was attenuated by methylation at the 3 ''- or 4 ''-position. On the other hand, (-)-epigallocatechin 3-O-gallate (EGCg) and (-)-catechin 3-O-gallate (Cg) exhibited weaker inhibitory activity than ECg, and (-)-epicatechin and (-)-epigallocatechin did not affect sSMase activity. Additionally, one synthetic catechin, (-)-3'-O-methylepigallocatechin 3-O-gallate (EGCg-3'-O-Me), showed the strongest inhibitory effect (IC50=1.7 mu m) on sSMase. This phenomenon was not observed for (-)-4'-O-methylepigallocatechin 3-O-gallate. These results suggest that the reduction potential, the presence of the galloyl residue at the C-3 position, and the steric requirement to interact with sSMase protein are important for effective inhibition of sSMase., Apr. 2016, 62, 2, 123, 129, Scientific journal
  • Refereed, JOURNAL OF FOOD PROTECTION, INT ASSOC FOOD PROTECTION, Inhibition of Morganella morganii Histidine Decarboxylase Activity and Histamine Accumulation in Mackerel Muscle Derived from Filipendula ulumaria Extracts, Yoko Nitta; Fumiko Yasukata; Noritoshi Kitamoto; Mikiko Ito; Motoyoshi Sakaue; Hiroe Kikuzaki; Hiroshi Ueno, Filipendula ulmaria, also known as meadowsweet, is an herb; its extract was examined for the prevention of histamine production, primarily that caused by contaminated fish. The efficacy of meadowsweet was assessed using two parameters: inhibition of Morganella morganii histidine decarboxylase (HDC) and inhibition of histamine accumulation in mackerel. Ellagitannins from F. ulmaria (rugosin D, rugosin A methyl ester, tellimagrandin II, and rugosin A) were previously shown to be potent inhibitors of human HDC; and in the present work, these compounds inhibited M. morganii HDC, with half maximal inhibitory concentration values of 1.5, 4.4, 6.1, and 6.8 respectively. Application of the extracts (at 2 wt%) to mackerel meat yielded significantly decreased histamine accumulation compared with treatment with phosphate-buffered saline as a control. Hence, F. ulmaria exhibits inhibitory activity against bacterial HDC and might be effective for preventing food poisoning caused by histamine., Mar. 2016, 79, 3, 463, 467, Scientific journal
  • Refereed, JOURNAL OF FUNCTIONAL FOODS, ELSEVIER SCIENCE BV, Anti-allergenic activity of polymethoxyflavones from Kaempferia parviflora, Shoko Kobayashi; Taro Kato; Toshiaki Azuma; Hiroe Kikuzaki; Keiko Abe, The inhibitory effects of 13 polymethoxyflavones (PMFs) obtained from Kaempferia paruiflora extracts were evaluated on RBL-2H3 cell degranulation. These flavones suppressed antigeninduced degranulation in RBL-2H3 cells. 5-Hydroxy-3,7,4',-trimethoxyflavone (KP02) and 5,3'-dihydroxy-3,7,4',-trimethoxyflavone (KP10) showed potent inhibitory activities. KP10 significantly suppressed calcium ionophores, such as A23187 and 2,5-ditert-butylhydroquinone, which promoted calcium influx from the outside of cells and from the endoplasmic reticulum, respectively. However, these activities did not fully explain the PMFs' effects. Immunoblot analysis of KPO2 and KP10 showed that the inhibitory effects were attributable to the downregulation of PLC gamma 1 kinase phosphorylation in the signaling pathways involved in degranulation. The phosphorylation levels of Syk were also suppressed by the addition of KP10. KP02 and KP10 treatment to the cells did not affect the high affinity IgE receptor (Fc epsilon RI) levels inside RBL-2H3 cells, but they suppressed the Fc epsilon RI levels in the plasma membrane. Therefore, K. parviflora PMFs have multiple degranulation mechanisms to suppress RBL-2H3 cells and would be beneficial for alleviating type I allergy symptoms. (C) 2014 Elsevier Ltd. All rights reserved., Mar. 2015, 13, 100, 107, Scientific journal
  • Refereed, FOOD HYDROCOLLOIDS, ELSEVIER SCI LTD, Sucrose release from agar gels: Correlation with sucrose content and rheology, Kun Yang; Zheng Wang; Tom Brenner; Hiroe Kikuzaki; Yapeng Fang; Katsuyoshi Nishinari, The effect of sucrose and agar concentrations on sucrose release from agar gels was investigated. It was found that the sucrose release ratio decreased with increasing sucrose concentration and with increasing fracture stress, which is consistent with previous findings. High correlation was found between the fracture stress, sucrose concentration and the sucrose release ratio. The total amount of sucrose released showed a maximum at a certain sucrose concentration, indicating that sucrose addition to enhance sweetness intensity may be ineffective or even counterproductive beyond a certain limit. (C) 2014 Elsevier Ltd. All rights reserved., Jan. 2015, 43, 1, 132, 136, Scientific journal
  • Refereed, FOOD HYDROCOLLOIDS, ELSEVIER SCI LTD, Sucrose release from agar gels: Effects of dissolution order and the network inhomogeneity, Kun Yang; Zheng Wang; Tom Brenner; Hiroe Kikuzaki; Yapeng Fang; Katsuyoshi Nishinari, The effect of sucrose addition prior to or following the dissolution of agar on sucrose release from 1 wt% agar gels was studied. The order of addition was found to affect the fracture stress, fracture strain and sucrose release ratio. At sucrose concentrations below 30%, the rheology (fracture stress and strain) and structure of the gels did not depend on the order of addition of sucrose and agar. At sucrose concentrations >40%, however, the network structure of gels where sucrose was dissolved before agar addition was weaker than that of gels where sucrose was added after agar dissolution. The weaker network structure resulted in lower fracture stress and strain values. For gels prepared by adding agar to sucrose solutions, both fracture stress and strain decreased with increasing sucrose concentration in the range 40-55%. The decrease in fracture stress and strain led to an increase in the sucrose release ratio. The sucrose release ratio decreased monotonously with increasing concentration of sucrose when agar was dissolved prior to sucrose addition. The findings are discussed in relation to recent publications on gel inhomogeneity. (C) 2014 Elsevier Ltd. All rights reserved., Jan. 2015, 43, 1, 100, 106, Scientific journal
  • Refereed, FOOD HYDROCOLLOIDS, ELSEVIER SCI LTD, The influence of agar gel texture on sucrose release, Zheng Wang; Kun Yang; Tom Brenner; Hiroe Kikuzaki; Katsuyoshi Nishinari, The relationship between sucrose release and texture of food gels was investigated by rheological measurements. Agar gels were selected as model food gel, and sucrose was used as a sweetener. Large deformation uniaxial compression tests were carried out at different compression speeds. The fracture stress increased with increasing agar concentration, while the concentration dependence of the fracture strain was different at different compression speeds. The size-distribution of fragments obtained after repeated compression-cycles was quantified using image analysis. The number and total surface area of fragments decreased with increasing agar concentration. Increasing the compression speed led to an initial increase followed by a monotonous decrease of both the number of fragments and their total surface area. The gel texture affected in a similar fashion both the sucrose release ratio and the total fragment surface area. The sucrose release ratio decreased with increasing agar concentration. (C) 2013 Elsevier Ltd. All rights reserved., May 2014, 36, 196, 203, Scientific journal
  • Refereed, PHYTOCHEMISTRY LETTERS, ELSEVIER SCIENCE BV, Glucosyl terpenates from the dried fruits of Prunus domestica L., Shin-ichi Kayano; Hiroe Kikuzaki; Sachiko Hashimoto; Kumi Kasamatsu; Takao Ikami; Nobuji Nakatani, The chemical structures of 9 compounds isolated from the dried fruits of Prunus domestica L. were elucidated on the basis of NMR and MS analyses. Each isolated compound was determined to be scopolin (1), (3-O-cis-p-coumaroyl-beta-D-fructofuranosyl)-(2 -> 1)-alpha-D-glucopyranoside (2), 1S-(4-beta-D-glucopyranosyl- 3-methoxyphenyl)-2R-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol (3), beta-D-glucopyranosyl 9-carboxy-8-hydroxy-2,7-dimethyl-2E, 4E-nonadienate (4), beta-D-glucopyranosyl 7-carboxy2- methyl-2E, 4E-octadienate (5), 8-hydroxy-2,7-dimethyl-2E, 4E-decadienedioic acid 1-beta-D-glucopyranyl ester 10-methyl ester (6), (3-O-trans-p-coumaroyl-beta-D-fructofuranosyl)-(2 -> 1)-alpha-D-glucopyranoside (7), b-D-glucopyranosyl cinnamate (8), and 2,7-dimethyl-2E, 4E-octadienedioic acid (9), respectively. Compounds 2, 3, 4, 7, 8, and 9 were isolated from P. domestica L. for the first time, and compounds 5 and 6 were novel glucosyl terpenates. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved., May 2014, 8, 132, 136, Scientific journal
  • Refereed, BIOLOGICAL & PHARMACEUTICAL BULLETIN, PHARMACEUTICAL SOC JAPAN, Increase in Secretory Sphingomyelinase Activity and Specific Ceramides in the Aorta of Apolipoprotein E Knockout Mice during Aging, Keiko Kobayashi; Eri Nagata; Kazuki Sasaki; Mariko Harada-Shiba; Shosuke Kojo; Hiroe Kikuzaki, Atherosclerosis is caused by many factors, one of which is oxidative stress. We recently demonstrated that systemic oxidative stress increased secretory sphingomyelinase (sSMase) activity and generated ceramides in the plasma of diabetic rats. In addition, we also showed that the total ceramide level in human plasma correlated with the level of oxidized low-density lipoprotein. To investigate the relationship between ceramide species and atherogenesis during aging, we compared age-related changes in ceramide metabolism in apolipoprotein E knock out mice (apoE(-/-)) and wild type mice (WT). Although the total plasma ceramide level was higher in apoE(-/-) than that in WT at all ages, it decreased with increasing age. sSMase activity increased at 65 weeks (w) of age in both strains of mice. When apoE(-/-) developed atherosclerosis at 15 w of age, C18:0, C22:0, and C24:0 ceramide levels in the apoE(-/-) aorta significantly increased. Furthermore, at 65 w of age C16:0 and C24:1 ceramide levels were significantly higher than those in WT. These results suggested that elevation in levels of specific ceramide species due to sSMase activity contributed to atherogenesis during aging., Jul. 2013, 36, 7, 1192, 1196, Scientific journal
  • Refereed, Food Chemistry, Inhibitory activity of Filipendula ulmaria constituents on recombinant human histidine decarboxylase, Yoko Nitta; Hiroe Kikuzaki; Toshiaki Azuma; Yuan Ye; Motoyoshi Sakaue; Yoshiki Higuchi; Hirohumi Komori; Hiroshi Ueno, Histidine decarboxylase (HDC) catalyses the formation of histamine, a bioactive amine. Agents that control HDC activity are beneficial for treating histamine-mediated symptoms, such as allergies and stomach ulceration. We searched for inhibitors of HDC from the ethyl acetate extract of the petal of Filipendula ulmaria, also called meadowsweet. Rugosin D, rugosin A, rugosin A methyl ester (a novel compound), and tellimagrandin II were the main components these 4 ellagitannins exhibited a non-competitive type of inhibition, with Ki values of approximately 0.35-1 μM. These Ki values are nearly equal to that of histidine methyl ester (Ki = 0.46 μM), an existing substrate analogue inhibitor. Our results show that food products contain potent HDC inhibitors and that these active food constituents might be useful for designing clinically available HDC inhibitors. © 2012 Elsevier Ltd. All rights reserved., 01 Jun. 2013, 138, 2-3, 1551, 1556, Scientific journal
  • Refereed, 日本給食経営管理学会誌, 日本給食経営管理学会, Attempt of energy education with estimation and measurement of electric power consumption during institutional food seryvice management practicum, KIKUZAKI Hiroe, May 2013, 7, 1, 31-42, 42
  • Refereed, FOOD CHEMISTRY, ELSEVIER SCI LTD, Isoflavone C-glycosides isolated from the root of kudzu (Pueraria lobata) and their estrogenic activities, Shin-ichi Kayano; Yoko Matsumura; Yoko Kitagawa; Mayumi Kobayashi; Asuka Nagayama; Nami Kawabata; Hiroe Kikuzaki; Yoshimi Kitada, The chemical structures of six isoflavones (1-6) isolated from the kudzu root (Pueraria lobata) were elucidated on the basis of the NMR and MS analyses to be four isoflavone C-glycosides as 6 ''-O-alpha-D-glucopyranosylpuerarin (1), puerarin (2), 3'-methoxypuerarin (3), 6 ''-O-alpha-D-apiofranosylpuerarin (4), and two aglycons as biochanin A (5) and formononetin (6), respectively. The estrogenic activities of isolated compounds and related isoflavones were evaluated using a yeast two-hybrid assay. Genistein exhibited the highest activity among the evaluated compounds at 10(-6) M followed by daidzein (10(-5) M), baiochanin A (5) (10(-5) M), daidzin (10(-5) M), 3',4',7-trihydroxyisoflavone (10(-3) M), and formononetin (6) (10(-2) M). The isoflavone C-glycosides (1-4) and 4',7-dimethoxyisoflavone showed no activities. In these results, it was found that the addition of 8-C-glucose or 3'-hydroxyl group to daidzein skeleton, or the substitution of hydroxyl functions to methoxyl groups, decreased the estrogenic activity of daidzein, on the other hand, the addition of 7-O-glucose moiety had no influence on the activity. (C) 2012 Elsevier Ltd. All rights reserved., Sep. 2012, 134, 1, 282, 287, Scientific journal
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Identification of a Novel Glycoside, Leptosin, as a Chemical Marker of Manuka Honey, Yoji Kato; Natsuki Umeda; Asuna Maeda; Daiki Matsumoto; Noritoshi Kitamoto; Hiroe Kikuzaki, As a preliminary study, we have found that honey from manuka (Leptospermum scoparium) in New Zealand inhibits myeloperoxidase (MPO) activity. In this study, using a chromatographic technique, we isolated two active compounds for MPO-inhibition from manuka honey. One is methyl syringate (MSYR), and the other was identified as a novel glycoside of MSYR, methyl syringate 4-O-beta-D-gentiobiose, which has been named "leptosin" after the genus Leptospermum. The amount of the glycoside ranged from 0.2 to 1.2 mu mol/g honey. Leptosin was only found in honeys from the Oceania region, and abundantly in manuka honey including jelly bush honey from Leptospermum polygalifolium in Australia. Therefore, leptosin may be a good chemical marker for manuka honey. Interestingly, the concentration of leptosin in manuka honey was positively correlated with the unique manuka factor (UMF) value, which is expressed as phenol equivalents of its bactericidal activity., Apr. 2012, 60, 13, 3418, 3423, Scientific journal
  • Refereed, FOOD CHEMISTRY, ELSEVIER SCI LTD, Antimutagenic and alpha-glucosidase inhibitory effects of constituents from Kaempferia parviflora, Toshiaki Azuma; Shin-ichi Kayano; Yoko Matsumura; Yotaro Konishi; Yasuo Tanaka; Hiroe Kikuzaki, The dichloromethane extract and ethyl acetate soluble fraction of Kaempferia parviflora showed antimutagenicity and alpha-glucosidase inhibitory activity. 7-Methoxyflavones were the major constituents of both extracts and demonstrated antimutagenic activity. In particular, 5,7-dimethoxyflavone (IC50 = 0.40 nmol/plate), 5,3'-dihydroxy-3,7,4'-trimethoxyflavone (0.40 nmol/plate), 3,5,7-trimethoxyflavone (0.42 nmol/plate) and 5-hydroxy-7-methoxyflavone (0.47 nmol/plate) showed potent antimutagenic activity. The results of structure-activity relationship studies suggest that methylation of the 5-hydroxy group enhanced activity. On the other hand, increasing the number of methoxy groups on the B ring caused a reduction in activity. In the case of an a-glucosidase inhibitory effect, 5,7,3',4'-tetramethoxyflavone exhibited the highest activity (IC50 = 20.4 mu M), followed by 5,7,4'-trimethoxyflavone (54.3 mu M) and 3,5,7,3',4'-pentamethoxyflavone (64.3 mu M). The results suggest that the a-glucosidase inhibitory activity was enhanced by the methoxy groups at the 5-, 3'- and 4'-position and was reduced by the methoxy group at the 3-position. (C) 2010 Elsevier Ltd All rights reserved., Mar. 2011, 125, 2, 471, 475, Scientific journal
  • Refereed, 日本給食経営管理学会誌, 日本給食経営管理学会, 大量調理施設におけるエネルギー消費意識の実態調査と管理栄養士養成課程の学内給食実習におけるエネルギー消費量「見える化」の試み, KIKUZAKI Hiroe, Mar. 2011, 5, 1, 29-37, 37
  • Refereed, Food Chemistry, Inhibitory Activity of Pimenta dioica Extracts and Constituents on Recombinant Human Histidine Decarboxylase, KIKUZAKI Hiroe; Yoko Nitta; Hiroe Kikuzaki; Hiroshi Ueno, Jan. 2009, 113, 445-449
  • Refereed, Phytochemistry, Phenolic Glycosides from Kaempferia parviflora, KIKUZAKI Hiroe; Toshiaki Azuma; Yasuo Tanaka; Hiroe Kikuzak, Oct. 2008, 69, 2743-2748, 2748
  • Refereed, JOURNAL OF NATURAL PRODUCTS, AMER CHEMICAL SOC, Phenolic glycosides from berries of Pimenta dioica, Hiroe Kikuzaki; Yoshiko Miyajima; Nobuji Nakatani, Four new phenolic glycosides, (27hydroxy-3-methoxy-5-allyl)phenyl beta-D-(6-O-E-sinapoyl)glucopyranoside (1), (1'R,5'R)-5-(5-carboxymethyl-2-oxocyclopentyl)-3Z-pentenyl beta-D-(6-O-galloyl)glucopyranoside (2), (S)-alpha-terpinyl [alpha-L-(2-O-galloyl)arabinofuranosyl]-(1 -> 6)-beta-D-glucopyranoside (3), and (R)-alpha-terpinyl [alpha-L-(2-O-galloyl)arabinofuranosyl]-(1 -> 6)-beta-D-glucopyranoside (4), were isolated from the berries of Pimenta dioica together with eight known flavonoids. The structures of 1-4 were elucidated on the basis of MS and NMR data and enzymatic hydrolysis. All four glycosides showed radical - scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals., May 2008, 71, 5, 861, 865, Scientific journal
  • Refereed, JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, WILEY-BLACKWELL, Ecdysteroids act as inhibitors of calf skin collagenase and oxidative stress, Robert Yawadio Nsimba; Hiroe Kikuzaki; Yotaro Konishi, Three new phytoecdysteroids have been isolated from the seeds of Chenopodium quinoa and structurally identified as 20,26-dihydroxy, 28-methyl ecdysone, 20,26-dihydroxy, 24(28)-dehydro ecdysone, and 20-hydroxyecdysone 22-glycolate using serial chromatographic and spectroscopic methods. Both previously reported compounds and newly identified phytoecdysteroids were evaluated for their inhibitory effect on calf skin collagenase, as this enzyme is involved in aging skin diseases. Their effectiveness on scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, as well as in chelating the iron metal ions was also investigated. All isolated compounds demonstrated a higher potency to inhibit this matrix metalloproteinase and to chelate the iron ion, both with respect to the number of carbonyl groups bearing their carbon skeleton, suggesting that their mechanism of action involves their ability to coordinate both ions (either the zinc ion of the catalytic domain of collagenase or the iron ion), acting as an electron donor. The DPPH-scavenging ability was hydroxyl dependent. Satisfactory results obtained from the enzyme in vitro experiment were further supported by the gel electrophoresis. These results indicate that ecdysteroids might be considered as potent chemical agents to prevent or delay both collagenase-related skin damages and oxidative stress. (C) 2008 Wiley Periodicals, Inc., 2008, 22, 4, 240, 250, Scientific journal
  • Refereed, FOOD CHEMISTRY, ELSEVIER SCI LTD, Antioxidant activity of various extracts and fractions of Chenopodium quinoa and Amaranthus spp. seeds, Robert Yawadio Nsimba; Hiroe Kikuzaki; Yotaro Konishi, The antioxidant potency of various extracts and fractions from Chenopodium quinoa and Amaranthus sp. was evaluated using three established methods, specifically the DPPH scavenging activity, FRAP, and P-carotene bleaching assays. Satisfying results were obtained, which lead to expect the use of these seeds as health-promoting ingredients. The antioxidant activity was less correlated to the phenolics content suggesting that non-phenolic compounds might play major free radicals scavenging activity in studied plant materials. (c) 2007 Elsevier Ltd. All rights reserved., Jan. 2008, 106, 2, 760, 766, Scientific journal
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, Chemical constituents of cape aloe and their synergistic growth-inhibiting effect on Ehrlich ascites tumor cells, Saeda Kametani; Akiko Kojima-Yuasa; Hiroe Kikuzaki; David Opare Kennedy; Mayumi Honzawa; Isao Matsui-Yuasa, The constituents of cape aloe were investigated after a preliminary screening of the growth-inhibiting effect on Ehrlich ascites tumor cells (EATC) of several extracts of this plant. Ten compounds were isolated from the dichloromethane (CH2Cl2) extract that showed the strongest activity, and their structures were elucidated as aloe-emodin (1), p-hydroxybenzaldehyde (2), p-hydroxyacetophenone (3), pyrocatechol (4), 10-oxooctadecanoic acid (5), 10-hydroxyoctadecanoic acid (6), methyl 10-hydroxyoctadecanoate (7), 7-hydroxy-2,5-dimethylchromone (8), furoaloesone (9), and 2-acetonyl-8-(2-furoylmethyl)-7-hydroxy-5-methylchromone (10) based on MS and various NMR spectroscopic techniques. Compounds 2-7 were isolated for the first time from cape aloe. Compounds 4-7 and 10 showed a significant growth-inhibiting effect, and compound 1 exhibited a remarkable synergistic effect on compounds 8-10, which was not observed with the treatment by each compound alone on EATC. These results suggest that the strong growth-inhibiting effect of the CH2Cl2 extract was dependent not on one compound alone, but on the synergistic effect from the combination of compound 1 and the other compounds., May 2007, 71, 5, 1220, 1229, Scientific journal
  • Refereed, J. Agric. Food Chem., Food Components Inhibiting Recombinant Human Histidine Decarboxylase Activity, KIKUZAKI Hiroe; Yoko Nitta; Hiroe Kikuzaki; Hiroshi Ueno, Feb. 2007, 55, 2, 299-304, 304
  • Refereed, 生活科学研究誌, 大阪市立大学大学院生活科学研究科, 給食経営管理実習における衛生管理に対するHACCP概念導入の教育効果について-作業過程における一般生菌数の推移と作業動線の実態調査から-, KIKUZAKI Hiroe, 栄養士法一部改正以前の旧カリキュラムで実施した給食管理実習と改正後の新カリキュラムで実施した給食経営管理実習において、HACCP(危害分析重要管理点)概念を導入した衛生教育を行うことが実際の実習の場にどのような影響を与えるかを検証した。使用設備の一般生菌数の推移はHACCP教育の有無にかかわらず、作業の清潔度に応じた推移を示した。手指の一般生菌数の推移を調べた結果からはHACCP教育の導入による手指の洗浄改善効果は認めなかった。作業動線および温度管理状況に対しては、HACCP教育の効果が顕著に認めた。, Mar. 2007, 5, 35, 44
  • Refereed, Journal of human life science, Osaka City University, 特定給食施設(病院)における人件費率、食材料費の実態, KIKUZAKI Hiroe, Dec. 2006, 5, 45-50, 50
  • Refereed, JOURNAL OF NATURAL PRODUCTS, AMER CHEMICAL SOC, Terpenoids and a diarylheptanoid from Zingiber ottensii, Kayo Akiyama; Hiroe Kikuzaki; Takako Aoki; Akiko Okuda; Nordin H. Lajis; Nobuji Nakatani, Four new terpenoids and a diarylheptanoid were isolated together with 16 known compounds from rhizomes of Zingiber ottensii. The structures of the new compounds were determined to be 1,10,10-trimethylbicyclo[7,4,0] tridecane-3,6-dione (1), (E)-14-hydroxy-15-norlabda-8(17), 12-dien-16-al (2), (E)-labda-8(17), 12,14-trien-15(16)-olide (3), (E)-14,-15,16- trinorlabda-8(17), 11-dien-13-oic acid (4), and rel-(3R, 5S)-3,5-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(3, 4-dihydroxyphenyl) heptane (5) by spectroscopic evidence., Nov. 2006, 69, 11, 1637, 1640, Scientific journal
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, Capsaicinol: Synthesis by allylic oxidation and its effect on TRPV1-expressing cells and adrenaline secretion in rats, Kenji Kobata; Takahito Iwasawa; Yusaku Iwasaki; Akihito Morita; Yuichi Suzuki; Hiroe Kikuzaki; Nobuji Nakatani; Tatsuo Watanabe, Capsaicinol is an ingredient of hot red pepper. In this study, we developed a novel method for capsaicinol synthesis and examined capsaicinol's physiological effects on capsaicin receptor (TRPV1)-related actions. Allylic oxidation of capsaicin by palladium acetate (Pd(OAc)(2)) resulted in the formation of (+/-)-capsaicinol acetate at a 7.2% yield in a single step. The effectiveness of (+/-)-capsaicinol in TRPV1 activation (EC50 = 1.1 mu M) was found to be weaker than that of capsaicin (EC50 = 0.017 mu M), whereas the efficacy of (+/-)-capsaicinol reached 15% of that of capsaicin. Intravenous administration of (+/-)-capsaicinol in anesthetized rats dose-dependently enhanced adrenaline secretion from the adrenal gland. The response to a 5mg/kg-dose of (+/-)-capsaicinol was comparable to that of a 0.05 mg/kg-dose of capsaicin. The relative pungency of capsaicinol to capsaicin was coincident with the relative effectiveness in. inducing these TRPV1-related actions., Aug. 2006, 70, 8, 1904, 1912, Scientific journal
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine, Chemical Constituents of Piper retrofractum Vahl and Their Antioxidant and Radical Scavenging Activities, KIKUZAKI Hiroe; Saeda Kametani; Hiroe Kikuzaki; Mayumi Honzawa; Nobuji Nakatani, Dec. 2005, 6, 566-573
  • Refereed, JOURNAL OF NATURAL PRODUCTS, AMER CHEMICAL SOC, Antioxidative constituents of Etlingera elatior, H Mohamad; NH Lajis; F Abas; AM Ali; MA Sukari; H Kikuzaki; N Nakatani, Phytochemical studies on the rhizomes of Etlingera elatior have resulted in the isolation of 1,7-bis(4-hydroxyphenyl)-2,4,6-heptatrienone (1), demethoxycurcumin (2), 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (3), 16-hydroxylabda-8(17),11,13-trien-15,16-olide (4), stigmast-4-en-3-one, stigmast-4-ene-3,6-dione, stigmast-4-en-6beta-ol-3-one, and 5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol. Compounds 1 and 4 are new, and their structures were elucidated by analysis of spectroscopic data. Diarylheptanoids 1-3 were found to inhibit lipid peroxidation in a more potent manner than alpha-tocopherol., Feb. 2005, 68, 2, 285, 288, Scientific journal
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine, Screening of Spice and Herb Extracts for Cytotoxic Activity against Ehrlich Ascites Tumor Cells and DPPH Radical Scavenging Activity, KIKUZAKI Hiroe; Saeda Kametani; Isao Matsui-Yuasa; Akiko Kojima-Yuasa; Tsutomu Arimura; David Opare Kennedy; Mayumi Honzawa; Hiroe Kikuzaki; Nobuji Nakatani, Dec. 2004, 5, 67-75, 75
  • Refereed, ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, VERLAG Z NATURFORSCH, DPPH free radical scavenger components from the fruits of Alpinia rafflesiana Wall. ex. Bak. (Zingiberaceae), H Mohamad; F Abas; D Permana; NH Lajis; AM Ali; MA Sukari; TYY Hin; H Kikuzaki; N Nakatani, The methanol extract of the dried ripe fruits of Alpinia rafflesiana was investigated for its DPPH free radical scavenger constituents. 2',3',4',6'-Tetrahydroxychalcone (7), which has never been isolated from natural sources was found to be most active as a DPPH free radical scavenger with the IC50 value of 55 mum. Other known compounds isolated from this species include 5,6-dehydrokawain (1), flavokawin B (2), 1,7-diphenyl-5-hydroxy-6-hepten-3- one (3), (-)-pinocembrin (4), cardamonin (5) and (-)-pinostrobin (6). The DPPH free radical scavenger compounds were detected using TLC autographic analysis. The percentage inhibition of DPPH free radical scavenging activity was measured on isolates (5-7) using colorimetric analysis., Nov. 2004, 59, 11-12, 811, 815, Scientific journal
  • Refereed, J. Agric. Food Chem., Constituents of the Leaves of Peucedanum japonicum Thunb. and Their Biological Activity, KIKUZAKI Hiroe; Masashi Hisamoto; Hiroe Kikuzaki; Nobuji Nakatani, Apr. 2004, 52, 445-450
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, A new bipyrrole and some phenolic constituents in prunes (Prunus domestica L.) and their oxygen radical absorbance capacity (ORAC), S Kayano; H Kikuzaki; T Ikami; T Suzuki; T Mitani; N Nakatani, Isolation and structural elucidation of prune constituents were performed and total 10 compounds were determined by NMR and MS analyses. A novel compound was identified to be 2-(5-hydroxymethyl-2',5'dioxo-2',3',4',5'-tetrahydro-l'H-1,3'-bipyrrole)carbalde- hyde, and 7 phenolic compounds were isolated from prunes for the first time. In addition, antioxidant activity of them was evaluated on the basis of the oxygen radical absorbance capacity (ORAC)., Apr. 2004, 68, 4, 942, 944, Scientific journal
  • Refereed, 食生活研究, 食生活研究会, 大量調理に使用する粉末出しのミネラル含量-学校給食から-, KIKUZAKI Hiroe, Apr. 2004, 24, 4, 40-46, 46
  • Refereed, J. Agric. Food Chem., Abscisic Acid Related Compounds and Lignans in Prunes (Prunus domestica L.) and Their Oxygen Radical Absorbance Capacity (ORAC), KIKUZAKI Hiroe; Hiroe Kikuzaki; Shin-ichi Kayano; Naoko Fukutsuka; Asuka Aoki; Kumi Kasamatsu; Yuka Yamasaki; Takahiko Mitani; Nobuji Nakatani, Mar. 2004, 52, 344-349
  • Refereed, BIOFACTORS, IOS PRESS, Antioxidant properties of gingerol related compounds from ginger, Y Masuda; H Kikuzaki; M Hisamoto; N Nakatani, Ginger (Zingiber officinale Roscoe) shows an antioxidant activity, and we have been engaging to determine the structures of more than 50 antioxidants isolated from the rhizomes of ginger. The isolated antioxidants are divided into two groups; gingerol related compounds and diarylheptanoids. In this study, structure-activity relationship of gingerol related compounds was evaluated. Gingerol related compounds substituted with an alkyl group bearing 10-, 12- or 14-carbon chain length were isolated from the dichloromethane extract of rhizomes using repeated chromatographic techniques. The antioxidant activities of these compounds were evaluated by the following measurements; 1) 1, 1 -diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2) inhibitory effect on oxidation of methyl linoleate under aeration and heating by the Oil Stability Index (OSI) method, and 3) inhibitory effect on oxidation of liposome induced by 2,2 -azobis(2-amidinopropane)dihydrochloride (AAPH). These results suggested that the substituents on the alkyl chain might contribute to both radical scavenging effect and inhibitory effect of autoxidation of oils, while inhibitory effects against the AAPH-induced peroxidation of liposome was somewhat influenced by the alkyl chain length; the antioxidant activity might be due to not only radical scavenging activity of antioxidants but also their affinity of the antioxidants to the substrates., 2004, 21, 1-4, 293, 296, Scientific journal
  • Refereed, BIOFACTORS, IOS PRESS, Antioxidative polyphenols from berries of Pimenta dioica, Y Miyajima; H Kikuzaki; M Hisamoto; N Nakatani, The ethyl acetate-soluble part of allspice, berries of Pimenta dioica, showed strong antioxidant activity and radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazy] (DPPH) radical. From the ethyl acetate-soluble part, two new compounds, 5-galloyloxy-3,4-dihydroxypentanoic acid and 5-(5-carboxymethyl-2-oxocyclopentyl)-3Z-pentenyl 6-O-galloyl-beta-D-glucoside were isolated together with 11 known polyphenols by repeated column chromatography. Their structures were elucidated on the basis of MS and various NMR spectroscopic data.All isolated compounds were evaluated for antioxidative effects on oxidation of methyl linoleate under aeration and heating, and on peroxidation of liposome induced by 2,1-azobis-(2-amidinopropane)dihydrocloride (AAPH) as a water-soluble initiator along with their radical -scavenging activity against DPPH. Quercetin and its glycosides showed remarkable activity for scavenging DPPH radical and inhibiting peroxidation of liposome. Two new compounds also exhibited strong DPPH radical -scavenging activity and inhibitory effect on the peroxidation of liposome as myricetin., 2004, 21, 1-4, 301, 303, Scientific journal
  • Refereed, BIOFACTORS, IOS PRESS, Antioxidant properties of prunes (Prunus domestica L.) and their constituents, SI Kayano; H Kikuzaki; NF Yamada; A Aoki; K Kasamatsu; Y Yamasaki; T Ikami; T Suzuki; T Mitani; N Nakatani, Prunes contain large amounts of phenolics and show high antioxidant activity. The aim of this study is to clarify the contents of caffeoylquinic acid (CQA) isomers, and to estimate the contribution of these isomers to the antioxidant activity of prunes. Furthermore, structural elucidation and evaluation of antioxidant activity of prune components were also performed. CQA isomers in prunes were quantified by HPLC analysis, and it has become apparent that prunes contain relatively high amount of 4-O-caffeoylquinic acid. The contribution of CQA isomers to the antioxidant activity of prunes was revealed to be 28.4% on the basis of oxygen radical absorbance capacity (ORAC); hence, it was indicated that residual ORAC is dependent on unknown antioxidant components. Total 28 compounds were isolated and their structures were elucidated by NMR and MS analyses. Four abscisic acid related compounds, a chromanon, and a bipyrrole were novel. Each CQA isomer in prunes showed high antioxidant activities when measured by the oil stability index (OSI) method, O-2(-) scavenging activity, and ORAC. Other isolated compounds such as hydroxycinnamic acids, benzoic acids, coumarins, lignans, and flavonoid showed high ORAC values. Furthermore, a novel chromanon indicated a remarkable synergistic effect on ORAC of CQA isomers., 2004, 21, 1-4, 309, 313, Scientific journal
  • Refereed, PHARMACEUTICAL BIOLOGY, SWETS ZEITLINGER PUBLISHERS, Antioxidant and antitumor promoting activities of the flavonoids from Hedychium thyrsiforme, Jasril; LY Mooi; NH Lajis; AM Ali; MA Sukari; AA Rahman; AG Othman; H Kikuzaki; N Nakatani, Five flavonoids, including 3,7,4'-trimethoxy-5-hydroxyflavone (1), 3,4'-dimethoxy-5,7-dihydroxyflavone (2), 5,7,4'-trimethoxy-3-hydroxyflavone (3), 3,5,7,4'-tetramethoxyflavone (4), and 7,4'-dimethoxy-3,5-dihydroxyflavone (5), were isolated from the rhizome of Hedychium thyrsiforme and assayed for antioxidant and antitumor promoting activities. The antioxidant assays showed that 5,7,4'-trimethoxy-3-hydroxyflavone, 7,4'-dimethoxy-3,5-dihydroxyflavone and 3,4'-dimethoxy-5,7-dihydroxyflavone had strong activities. Only two compounds, 5,7,4'-trimethoxy-3-hydroxyflavone and 7,4'-dimethoxy-3,5-dihydroxyflavone, were found to be strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavengers with fifty percent inhibition concentration (IC50) values of 92 and 119 muM, respectively. Antitumor promoting assays indicated that all the flavonoids showed strong inhibition activity towards Epstein-Barr virus (EBV) activation in Raji cells., Oct. 2003, 41, 7, 506, 511, Scientific journal
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Comparison of antioxidative properties of carbazole alkaloids from Murraya koenigii leaves, Y Tachibana; H Kikuzaki; NH Lajis; N Nakatani, A new dimeric carbazole alkaloid, 8,10'-[3,3',11,11'-tetrahydro-9,9'-dihydroxy-3,3',5,8'-tetramethyl-3,3'-bis(4-methyl-3-pentenyl)]bipyrano[3,2-a]carbazole (12), was isolated from the CH2Cl2 extract of Murraya koenigii together with six known carbazole alkaloids, koenimbine (6), O-methylmurrayamine A (7), O-methylmahanine (8), isomahanine (9), bismahanine (10), and bispyrayafoline (11). Their structures were determined on the basis of H-1 and C-13 NMR spectroscopic and mass spectrometric (MS) data. The antioxidative properties of 12 carbazole alkaloids isolated from leaves of M. koenigii were evaluated on the basis of the oil stability index together with their radical scavenging ability against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. On the basis of the lag time to reach a steady state, the 12 carbazoles were classified into three groups. It is suggested that an aryl hydroxyl substituent on the carbazole rings plays a role in stabilizing the thermal oxidation and rate of reaction against DPPH radical., Oct. 2003, 51, 22, 6461, 6467, Scientific journal
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine, Quantification of Quinic Acid in Prunes (Prunus domestica L.) by HPLC Analysis, KIKUZAKI Hiroe; Shin-ichi Kayano; Takahiko Mitani; Hirotaka Yamamoto; Takao Ikami; Hiroe Kikuzaki; Tomoo Suzuki; Nobuji Nakatani, Oct. 2003, 4, 639-644
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Antioxidant compounds from the leaves of Peucedanum japonicum Thunb, M Hisamoto; H Kikuzaki; H Ohigashi; N Nakatani, Seventeen compounds were isolated from the n-butanol soluble fraction of the leaves of Peucedanum japonicum Thunb. On the basis of MS and various NMR spectroscopic techniques, the structures of the isolated compounds were determined as isoquercitrin (1), rutin (2), 3-O-caffeoylquinic acid (3), 4-O-caffeoylquinic acid (4), 5-O-caffeoylquinic acid (5), cnidioside A (6), praeroside II (7), praeroside III (8), apterin (9), esculin (10), (R)-peucedanol (11), (R)-peucedanol 7-O-beta-D-glucopyranoside (12), L-tryptophan (113), uracil (114), guanosine (115), uridine (116), and thymidine (117). All compounds except 11 and 12 were isolated for the first time from P. japonicum. Several isolated compounds were quantified by high-performance liquid chromatography analysis. In addition, all isolated compounds were examined for radical scavenging on 1,1-diphenyl-2-picrylhydrazyl radical and for inhibition of oxidation of liposome induced by 2,2'-azobis(2-amidinopropane)dihydrochloride. Compounds 2-5 were found to be the major potent constituents, which contribute to the antioxidant activity of P. japonicum leaves., Aug. 2003, 51, 18, 5255, 5261, Scientific journal
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine, Conformational Isomers of 3-O-Caffeoylquinic Acid Isolated from Prunes (Prunus domestica L.) and Their Antioxidant Activity, KIKUZAKI Hiroe; Shin-ichi Kayano; Hiroe Kikuzaki; Takahiko Mitani; Nobuji Nakatani, Aug. 2003, 4, 478-484
  • Refereed, J. Toxicol. Pathol., Lack of Inhibitory Effects of Coumaperine from Pepper on the Promotion Stage of Chemical Hepatocarcinogenesis in the Rat, KIKUZAKI Hiroe; Mitsuaki Kitano; Yutaka Ariki; Jutaro Wada; Yoo Tanabe; Hiroe Kikuzaki; Nobuji Nakatani; Hideki Wanibuchi; Keiichirou Morimura; Takayoshi Hidaka; Shoji Fukushima, Aug. 2003, 16, 161-164
  • Refereed, Pharmaceutical Biology, The Antioxidative Components from Alpinia nutans, KIKUZAKI Hiroe; M. Habsah; N.H. Lajis; A.M. Ali; M.A. Sukari; Y.Y. Hin; H. Kikuzaki; N. Nakatani, Jul. 2003, 41, 7-9
  • Refereed, J. Agric. Food Chem., Quantitative Evaluation of Antioxidant Components in Prunes (Prunus domestica L.), KIKUZAKI Hiroe; Shin-ichi Kayano; Naoko Fukutsuka Yamada; Tomoo Suzuki; Takao Ikami; Koichiro Shioaki; Hiroe Kikuzaki; Takahiko Mitani; Nobuji Nakatani, May 2003, 51, 1480-1485, 1485
  • Refereed, Journal of human life science, Osaka City University, 沖縄産香辛植物の食中毒菌増殖抑制作用, KIKUZAKI Hiroe, Apr. 2003, 1, 11-16, 16
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Sphingolipid and other constituents from almond nuts (Prunus amygdalus Batsch), SM Sang; H Kikuzaki; K Lapsley; RT Rosen; N Nakatani; CT Ho, One sphingolipid, 1-O-beta-D-glucopyranosyl-(2S,3R,4E,8Z)-2-[(2R)-2-hydroxyhexadecanoylamino]-4,8-octadecadiene-1,3-diol, and four other constituents, beta-sitosterol, daucosterol, uridine, and adenosine, have been isolated from the nuts of almond (Prunus amygdalus). Complete assignments of the proton and carbon chemical shifts for the sphingolipid were accomplished on the basis of high-resolution 1D and 2D NMR. data. All of these compounds are being reported from almond nuts (P. amygdalus) for the first time., Jul. 2002, 50, 16, 4709, 4712, Scientific journal
  • Refereed, Z. Naturforsch, Antifungal Garcinia Acid Esters from the Fruits of Garcinia atroviridis, KIKUZAKI Hiroe; Mukram M. Mackeen; Abdul Manaf Ali; Nordin Hj. Lajis; Kazuyoshi Kawazu; Hiroe Kikuzaki; Nobuji Nakatani, May 2002, 57c, 291-295
  • Refereed, J. Agric. Food Chem., Antixoidant Activity of Prune (Prunus domestica L.) Constituents and a New Synergist, KIKUZAKI Hiroe; Shin-ichi Kayano; Hiroe Kikuzaki; Naoko Fukutsuka; Takahiko Mitani; Nobuji Nakatani, May 2002, 50, 3708-3712
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Antioxidant activity of chemical components from sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index method, K Miura; H Kikuzaki; N Nakatani, A new abietane diterpenoid, 12-O-methyl carnosol (2), was isolated from the leaves of sage (Salvia officinalis L.), together with 11 abietane diterpenoids, 3 apianane terpenoids, 1 anthraquinone, and 8 flavonoids. Antioxidant activity of these compounds along with 4 flavonoids isolated from thyme (Thymus vulgaris L.) was evaluated by the oil stability index method using a model substrate oil including methyl linoleate in silicone oil at 90 degreesC. Carnosol, rosmanol, epirosmanol, isorosmanol, galdosol, and carnosic acid exhibited remarkably strong activity, which was comparable to that of alpha-tocopherol. The activity of miltirone, atuntzensin A, luteolin, 7-O-methyl luteolin, and eupafolin was comparable to that of butylated hydroxytoluene. The activity of these compounds was mainly due to the presence of ortho-dihydroxy groups. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of these compounds showed the similar result., Mar. 2002, 50, 7, 1845, 1851, Scientific journal
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Antioxidant properties of ferulic acid and its related compounds, H Kikuzaki; M Hisamoto; K Hirose; K Akiyama; H Taniguchi, Antioxidant activity of 24 ferulic acid related compounds together with 6 gallic acid related compounds was evaluated using several different physical systems as well as their radical scavenging activity. The radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) decreased in the order caffeic acid > sinapic acid > ferulic acid > ferulic acid esters > p-coumaric acid. In bulk methyl linoleate, test hydroxycinnamic acids and ferulic acid esters showed antioxidant activity in parallel with their radical scavenging activity. In an ethanol-buffer solution of linoleic acid, the activity of test compounds was not always associated with their radical scavenging activity. Ferulic acid was most effective among the tested phenolic acids. Esterification of ferulic acid resulted in increasing activity. The activity of alkyl ferulates was somewhat influenced by the chain length of alcohol moiety. When the inhibitory effects of alkyl ferulates against oxidation of liposome induced by AAPH were tested, hexyl, octyl, and 2-ethyl-1-hexyl ferulates were more active than the other alkyl ferulates. Furthermore, lauryl gallate is most effective among the tested alkyl gallates. These results indicated that not only the radical scavenging activity of antioxidants, but also their affinity with lipid substrates, might be important factors in their activity., Mar. 2002, 50, 7, 2161, 2168, Scientific journal
  • Refereed, JOURNAL OF NATURAL PRODUCTS, AMER CHEMICAL SOC, New flavonol-phenylbutadiene adducts from the leaves of Alpinia flabellata, H Kikuzaki; S Tesaki, Two new flavonol-phenylbutadiene adducts, rel-5-hydroxy-7,4-dimethoxy-2"S-(2,4,5-trimethoxy-E-styryl)tetrahydrofuro[4"R,5"R:2,3]flavanonol (1) and rel-5-hydroxy-7,4'-dimethoxy-3"S-(2,4,5-trimethoxy-E-styryl)tetrahydrofuro[4"R,5"R:2,3]flavanonol (2), were isolated from the leaves of Alpinia flabellata, along with three known compounds, 2,4,5-trimethoxybenzoic acid, 2,4,5-trimethoxycinnamic acid, and 5-hydroxy-3,7,4'-trimethoxyflavone. The structures of 1 and 2 were determined by spectroscopic interpretation., Mar. 2002, 65, 3, 389, 391, Scientific journal
  • Refereed, Jpn. J. Food Chem., Antibacterial Activity of Essential Oils and Oleoresins of Spices and Herbs against Pathogens Bacteria in Upper Airway Respiratory Tract, KIKUZAKI Hiroe; Yasuo Tanaka; Hiroe Kikuzaki; Nobuji Nakatani, Feb. 2002, 9, 67-76, 76
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine, Antioxidant Activity of Tropical Plant Leaves Traditionally Used for Food Preservation in Okinawa, KIKUZAKI Hiroe; Masashi Hisamoto; Hiroe Kikuzaki; Sigetomo Yonemori; Nobuji Nakatani, Jan. 2002, 3, 63-68, 68
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, Apianane terpenoids from Salvia officinalis, K Miura; H Kikuzaki; N Nakatani, Three apianane terpenoids, rel-(5S, 6S, 7S, 10R, 12S, 13R)-7-hydroxyapiana-8,14-diene-11,16-dion-(22,6)-olide, rel-(5S, 6S, 7R, 10R, 12S, 13R)-7-hydroxyapiana-8,14-diene-11,16-dion-(22,6)-olide and rel-(5S, 6S, 7S, 10R, 12R, 13S)-7-hydroxyapiana-8,14diene-11,16-dion-(22,6)-olide were isolated from the leaves of Salvia officinalis, together with 15 known compounds, salvigenin, lupeol, beta -sitosterol, stigmasterol, physcion, carnosol, rosmadial, rosmanol, epirosmanol, isorosmanol, columbaridione, atuntzensin A, miltirone, carnosic acid and 12-O-methyl carnosic acid. Their structures were established on the basis of spectral evidence. (C) 2001 Published by Elsevier Science Ltd., Dec. 2001, 58, 8, 1171, 1175, Scientific journal
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Antioxidative activity of carbazoles from Murraya koenigii leaves, Y Tachibana; H Kikuzaki; NH Lajis; N Nakatani, The antioxidative properties of the leaves extracts of Murraya koenigii using different solvents were evaluated based on the oil stability index (OSI) together with their radical scavenging ability against 1-1-diphenyl-2-picrylhydrazyl (DPPH). The methylene chloride (CH2Cl2) extract and the ethyl acetate (EtOAc) soluble fraction of the 70% acetone extract significantly prolonged the OSI values comparable to those of alpha -tocopherol and BHT. Five carbazole alkaloids were isolated from the CH2Cl2 extract and their structures were identified to be euchrestine B (1), bismurrayafoline E (2), mahanine (3), mahanimbicine (4), and mahanimbine (5) based on H-1 and C-13 NMR and mass (MS) spectral data. The OSI value of carbazoles at 110 degreesC decreased in the order 1 and 3 > alpha -tocopherol > BHT > 2 > 4, 5 and control. It is' assumed that compounds 1 and 3 contributed to the high OSI. value of the CH2Cl2 extract of M. koenigii. The DPPH radical scavenging activity for these carbazoles-was in the order ascorbic acid > 2 > 1, 3 and alpha -tocopherol > BHT > 4 and 5., Nov. 2001, 49, 11, 5589, 5594, Scientific journal
  • Refereed, J. Nat. Prod., Furanosesquiterpenoids of Commiphora myrrha, KIKUZAKI Hiroe; Nanqun Zhu; Hiroe Kikuzaki; Shuqun Sheng; Shengmin Sang; Mohamed M. Rafi; Mingfu Wang; Nobuji Nakatani; Robert S. DiPaola; Robert T. Rosen; Chi-Tang Ho, Nov. 2001, 64, 1460-1462
  • Refereed, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, CENTER ACADEMIC PUBL JAPAN, Antibacterial compounds of licorice against upper airway respiratory tract pathogens, Y Tanaka; H Kikuzaki; S Fukuda; N Nakatani, The antibacterial activity of compounds obtained from licorice was measured against upper airway respiratory tract bacteria such as Streptococcus pyogenes. Haemophilus influenzae and Moraxella catarrhalis. Among the tested compounds, licoricidin exhibited the highest activity against all tested microorganisms with an MIC of 12.5,mug/mL. Three coumarin derivatives, glycyrol, glycyrin and glycycoumarin also showed antibacterial activity., Jun. 2001, 47, 3, 270, 273, Scientific journal
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Ecdysteroids of Quinoa seeds (Chenopodium quinoa Willd.), N Zhu; H Kikuzaki; BC Vastano; N Nakatani; MV Karwe; RT Rosen; CT Ho, Quinoa (Chenopodium quinoa) is a hardy and nutritious Latin American pseudo-cereal. Studies on the seeds led to the isolation of five ecdysteroids using column chromatography. Their structures were determined as ecdysterone, makisterone A, 24-epi-makisterone A, 24(28)-dehydromakisterone A. and 20,26-dihydroxyecdysone by spectroscopic methods. This study demonstrates that quinoa seeds are a source of ecdysteroids, which were reported to be molting hormones in insects., May 2001, 49, 5, 2576, 2578, Scientific journal
  • Refereed, JOURNAL OF NATURAL PRODUCTS, AMER CHEMICAL SOC, New constituents of the leaves of Alpinia flabellata, S Tesaki; H Kikuzaki; S Yonemori; N Nakatani, As a part of our studies on the constituents of plants belonging to the family Zingiberaceae,(1-5) we have focused on Alpinia flabellata Ridley, which is a rare species in Japan. The leaves of this plant have been used as a wrapping and flavoring material for foods on the islands of Iriomote and Ishigaki, Okinawa, which is the southernmost part of Japan. Only two studies on the isolation of several phenylbutenoids from the rhizomes of A. flabellata have been reported.(6.7) We have reported previously the isolation and structural elucidation of three phenylbutanoid dimers from the leaves of A. flabellatas(8) and now describe the isolation and characterization of a new phenylbutenal (1) and a novel labdane diterpene (2) adducted by a phenylbutenoid., Apr. 2001, 64, 4, 515, 517, Scientific journal
  • Refereed, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, CENTER ACADEMIC PUBL JAPAN, 1,1-diphenyl-2-picrylhydrazyl radical-scavenging active compounds from greater cardamom (Amomum subulatum Roxb.), H Kikuzaki; Y Kawai; N Nakatani, Constituents of the fruits of greater cardamom (Amomum subulatum) were fractionated into three fractions, the dichloromethane extract, and the ethyl acetate-soluble and water-soluble fractions of the 70% aqueous acetone extract, The ethyl acetate-soluble fraction showed a high radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH). Four compounds were isolated from the ethyl acetate-soluble fraction, and their structures were ascribed to protocatechualdehyde (1), protocatechuic acid (2), 1,7-bis(3,4-dihydroxyphenyl)hepta-4E, 6E-dien-3-one (3) and 2,3, 7-trihydroxy-5-(3,4-dihydroxy-E-styryl)-6,7,8,9 -tetrahydro-5H-benzocycloheptene (4) on the basis of spectroscopic evidence. This is the first isolation of these compounds from greater cardamom. In particular, 4 was a new type of cyclic diarylheptanoid. DPPH radical-scavenging activity of these compounds was measured by colorimetric analysis. Compounds 1 and 3 showed stronger activity than such natural antioxidants as alpha -tocopherol and L-ascorbic acid. Compounds 2 and 4 were comparable to alpha -tocopherol and L-ascorbic acid., Apr. 2001, 47, 2, 167, 171, Scientific journal
  • Refereed, JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, AMER OIL CHEMISTS SOC A O C S PRESS, Establishment of a model substrate oil for antioxidant activity assessment by Oil Stability Index method, N Nakatani; Y Tachibana; H Kikuzaki, A model substrate oil using methyl linoleate was established for the determination of the antioxidant activity by Oil Stability Index (OSI) method. OSI values for methyl linoleate with different concentrations (5-100%) in silicone oil were measured at different temperatures (70-120 degreesC), As the temperature increased, the OSI value decreased in each concentration of methyl linoleate. Optimal temperature and concentration of methyl linoleate were established. The effect Of concentration of antioxidants, alpha -tocopherol, and butylated hydroxytoluene on OSI values for 10% methyl linoleate model oil was measured at 90, 100, 110, and 120 degreesC, The logarithmic reiationship between temperature and OSI using model substrate oil was similar to that of soybean oil. Furthermore, application of some spice extracts to this model oil system was carried out to give results that compared well with those available in the literature. Thus, the procedure using methyl linoleate-silicone oil as a model substrate oil is available for evaluating the antioxidant activity by the OSI method., Jan. 2001, 78, 1, 19, 23, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, Phenylbutanoid dimers from the leaves of Alpinia flabellata, H Kikuzaki; S Tesaki; S Yonemori; N Nakatani, Three phenylbutanoid dimers, cis- and trans-1-(2,4,5-trimethoxy-E-styryl)-2-(2,4,5-trimethoxy-Z-styryl)cyclobutane and 1,2- bis(2,4,5-trimethoxy-Z-styryl)- cyclobutane, were isolated from the leaves of Alpinia flabellata Ridley, together with three known compounds (2,4,5-trimethoxybenzaldehyde, 2,4,5-trimethoxycinnamaldehyde and 3,5-dihydroxy-7,4'-dimethoxyflavone). The structures of these compounds were determined by spectroscopic analysis. (C) 2001 Elsevier Science Ltd. All rights reserved., Jan. 2001, 56, 1, 109, 114, Scientific journal
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine, Antibacterial Activity of Phenolic Constituents Isolated from Alpinia flabellata and Their Regulating Effect on Ovalbumin Transport in Human Intestinal Caco-2 Cells, KIKUZAKI Hiroe; Shoko Tesaki; Hiroe Kikuzaki; Soichi Tanabe; Michiko Watanabe; Nobuji Nakatani, Jan. 2001, 2, 106-111, 110
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Identification, quantitative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L.), N Nakatani; S Kayano; H Kikuzaki; K Sumino; K Katagiri; T Mitani, Neochlorogenic acid (3-CQA) and cryptochlorogenic acid (4-CQA), isolated from prune (Prunus domestica L.), were identified by NMR and MS analyses. In addition, the quantity of chlorogenic acid isomers in prune were measured by HPLC. These isomers, 3-CQA, 4-CQA, and chlorogenic acid (5-CQA), were contained in the ratio 78.7:18.4:3.9, respectively. 4-CQA was identified and quantified in prune for the first time, and relatively high amounts of this isomer were characteristic. Antioxidative activities of the chlorogenic acid isomers, such as scavenging activity on superoxide anion radicals and inhibitory effect against oxidation of methyl linoleate, were also evaluated. Each isomer showed antioxidative activities which were almost the same., Nov. 2000, 48, 11, 5512, 5516, Scientific journal
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine, Antibacterial Activity of Aliphatic Long Chain Compounds against Upper Airway Respiratory Tract Bacteria, KIKUZAKI Hiroe; Yasuo Tanaka; Seiji Fukuda; Hiroe Kikuzaki; Nobuji Nakatani, Sep. 2000, 1, 777-780
  • Refereed, 微量栄養素研究, 微量栄養素研究会事務局, アガリクス(Agaricus blazei)子実体の抗酸化成分および多糖の研究, KIKUZAKI Hiroe, Sep. 2000, 17, 83-90, 90
  • Refereed, JAPANESE JOURNAL OF CANCER RESEARCH, BUSINESS CENTER ACADEMIC SOCIETIES JAPAN, Chemopreventive effects of coumaperine from pepper on the initiation stage of chemical hepatocarcinogenesis in the rat, M Kitano; H Wanibuchi; H Kikuzaki; N Nakatani; S Imaoka; K Funae; S Hayashi; S Fukushima, This study was designed to investigate the chemopreventive action of three natural products, coumaperine, aurapten and an extract from rosemary, against the initiation stage of rat hepatocarcinogenesis. Coumaperine has been isolated from white pepper as a naturally occurring antioxidative agent, but its potential modifying effects on carcinogenesis remain unclear. In experiment 1, a modification of the model developed by Tsuda et al. was applied, with assessment of numbers and areas of induced glutathione S-transferase placental form (GST-P)-positive hepatocellular foci in male F344 rats. Coumaperine, aurapten and the extract from rosemary were administered i.g. at 190 mg/kg/day once daily for 5 days with initiation by diethylnitrosamine (DEN) on day 4 (20 mg/kg, i.p.). Numbers and areas of GST-P-positive foci in each group given test chemicals tended to be decreased as compared to the vehicle control group values, significance being achieved for number with coumaperine. Experiment 2 was planned to investigate the mechanism of the inhibitory effects of coumaperine. Livers at 8 h after initiation by DEN were examined with coumaperine administered at 100 mg/kg/day once daily for 3 days. Proliferating cell nuclear antigen (PCNA)positive cells tended to be decreased as compared to the vehicle control, but no effects on apoptosis or cytochrome P-450 (CYP) 2E1 expression were apparent. Our results suggest that coumaperine provides protection against initiation of hepatocarcinogenesis, and that this is related to inhibition of cell proliferation., Jul. 2000, 91, 7, 674, 680, Scientific journal
  • Refereed, Journal of Natural Products, Novel glycosides from noni (Morinda citrifolia), Mingfu Wang; Hiroe Kikuzaki; Yi Jin; Nobuji Nakatani; Nanqun Zhu; Katalin Csiszar; Charles Boyd; Robert T. Rosen; Geetha Ghai; Chi-Tang Ho, Three new glycosides were isolated from the fruits of noni (Morinda citrifolia). Their structures were determined to be 6-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose (1), 6-O-(β-D-glucopyranosyl)-1-O-hexanoyl-β-D-glucopyranose (2), and 3-methylbut-3-enyl 6-O-β-D-glucopyranosyl-β-D-glucopyranoside (3) using MS and NMR methods., 2000, 63, 8, 1182, 1183, Scientific journal
  • Refereed, JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, AMER OIL CHEMISTS SOC A O C S PRESS, Constituents of Amomum tsao-ko and their radical scavenging and antioxidant activities, TS Martin; H Kikuzaki; M Hisamoto; N Nakatani, Constituents of the fruit of Amomum tsao-ko were investigated following a preliminary screening of the antioxidant activity of several extracts of the fruit of this plant that showed that the dichloromethane extract and the ethyl acetate-soluble and water-soluble fractions of the 70% aqueous acetone extract had higher activity than alpha-tocopherol and butylated hydroxytoluene (BHT). Eleven compounds were isolated from the ethyl acetate-soluble fraction, and their structures were elucidated as (+)-hannokinol (1), meso-hannokinol (2), (+)-epicatechin (3), (-)-catechin (4), beta-sitosterol (5), beta-sitosterol 3-O-glucoside (6), 2,6-dimethoxyphenol (7), protocatechualdehyde (8), protocatechuic acid (9), vanillic acid (10), and beta-hydroxybenzoic acid (11) based on mass and various nuclear magnetic resonance (NMR) spectroscopic techniques. This is the first isolation of epicatechin and catechin from the genus Amomum. The radical scavenging activity of the isolated compounds was evaluated using 1,1-diphenyl-2-picryIhydrazyl (DPPH) and colorimetric and electron spin resonance (ESR) analyses. The antioxidant activity of the compounds was also determined based on the oxidative stability index (OSI). The catechins and catechol derivatives showed strong activities in both the DPPH radical scavenging activity and antioxidant activity assays., Jun. 2000, 77, 6, 667, 673, Scientific journal
  • Refereed, ITE Letters on Batteries, New Technologies & Medicine,, Antibacterial Compounds from Nutmeg against Upper Airway Respiratory Tract Bacteria, KIKUZAKI Hiroe; Yasuo Tanaka; Seiji Fukuda; Hiroe Kikuzaki; Nobuji Nakatani, May 2000, 1, 412-417
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Isolation and structural elucidation of two new glycosides from sage (Salvia officinalis L.), MF Wang; H Kikuzaki; NQ Zhu; SM Sang; N Nakatani; CT Ho, Six compounds, 1-O-(2,3,4-trihydroxy-3-methyl)butyl-6-O-feruloyl-beta-D-glucopyranoside, ethyl beta-D-glucopyranosyl tuberonate, p-hydroxybenzoic acid, (-)-hydroxyjasmonic acid, caffeic acid, and 4-hydroxyacetophenone 4-O-[5-O-(3,5-dimethoxy-4-hydroxybenzoyl)-beta-D-apiofrunosyl]-(1-->2)-beta-D-glucopyranoside, were isolated from the n-butanol-soluble fraction of sage leaf extracts. Their structures were determined by spectral methods (MS, NMR, and BD-NMR), and their antioxidant activities were measured. Among them, two new glycosides were elucidated. All of these compounds showed DPPH free radical scavenging activity at the concentration of 30 mM, and caffeic acid was the most active compound., Feb. 2000, 48, 2, 235, 238, Scientific journal
  • Refereed, Journal of Ethnopharmacology, Screening of Zingiberaceae Extracts for Antimicrobial and Antioxidant Activities, KIKUZAKI Hiroe; M. Habsah; M. Amran; M.M. Mackeen; N.H. Lajis; H. Kikuzaki; N. Nakatani; A.A. Rahman, Ghafar; A.M. Ali, Feb. 2000, 72, 403-410
  • Refereed, J. Nat. Prod, Galloylglucosides from Berries of Pimenta dioica, KIKUZAKI Hiroe; Hiroe Kikuzaki; Akemi Sato; Yoko Mayahara; Nobuji Nakatani, Jan. 2000, 63, 749-752, 752
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, Antioxidative phenylpropanoids from berries of Pimenta dioica, H Kikuzaki; S Hara; Y Kawai; N Nakatani, A phenylpropanoid, threo-3-chloro-1-(4-hydroxy-3-methoxy phenyl)propane-1,2-diol, was isolated from the berries of Pimenta dioica together with five known compounds. eugenol, 4-hydroxy-3-methoxycinnamaldehyde, 3,4-dimethoxycinnamaldehyde, vanillin and 3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol. In addition, the stereochemistry of 3-(4-hydroxy-3-methoxyphenyl)propane- 1,2-diol was determined. The phenylpropanoids inhibited autoxidation of linoleic acid in a water-alcohol system. (C) 1999 Elsevier Science Ltd. All rights reserved., Dec. 1999, 52, 7, 1307, 1312, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, Two C-21-steroidal glycosides isolated from Cynanchum stauntoi, NQ Zhu; MF Wang; H Kikuzaki; N Nakatani; CT Ho, Studies on the roots of Cynanchum stauntoi led to the isolation of two C-21-steroidal glycosides, formally named stauntosides A and B. Their structures were elucidated on the basis of spectroscopic evidence, especially that from analysis of 2D-NMR spectra. They were found to possess an unusual skeleton and were identified as stauntogenin 3-O-alpha-L-diginopyranosyl-(1-4)beta-L-cymaropyranosyl-(1-4)-beta-D-digitoxopyranosyl-(1-4)-beta-D-thevetopyranoside and stauntogenin 3-O-alpha-L-cymaropyranosyl-(1-4)-beta-D-digitoxopyranosyl-(1-4)-beta-D-3-demethyl-2-deoxy-thevetopyranoside (C) 1999 Elsevier Science Ltd. All rights reserved., Dec. 1999, 52, 7, 1351, 1355, Scientific journal
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Novel trisaccharide fatty acid ester identified from the fruits of Morinda citrifolia (Noni), MF Wang; H Kikuzaki; K Csiszar; CD Boyd; A Maunakea; SFT Fong; G Ghai; RT Rosen; N Nakatani; CT Ho, Two known glycosides and a novel trisaccharide fatty acid ester were isolated from the n-butanol-soluble fraction of the fruits of Morinda citrifolia (noni). Structure determination was carried out by spectral techniques such as MS, IR, NMR, and BD-NMR. The novel trisaccharide fatty acid ester was elucidated as 2,6-di-O-(beta-D-glucopyranosyl)-1-O-octanoyl-beta-D-glucopyranose. The known compounds were identified as rutin and asperulosidic acid., Dec. 1999, 47, 12, 4880, 4882, Scientific journal
  • Refereed, Tropical Agriculture and Development, Japanese Society for Tropical Agriculture, 二倍体および四倍体ショウガの辛味成分と官能検査による辛味性評価ならびにメタノール抽出物の抗酸化性, KIKUZAKI Hiroe, The diploid and tetraploid types of the ginger cultivars, Kintoki, Oshoga and Sanshu, were studied in relation to the gingerol content, antioxidative activity and pungency sensory evaluation. The tetraploid ginger type was derived from the respective diploid ginger type by shoot tip culture. Gingerols are major pungent constituents in natural ginger. Among the three ginger cultivars, the total gingerol content of the tetraploid type was much higher than that of the respective diploid type and, especially, the 10-gingerol (10G) content of the tetraploid type showed a remarkable increase. Both diploid and tetraploid types of the cultivar Kintoki contained the highest level of gingerol among the respective types of the three ginger cultivars studied. Sensory evaluation of the pungency of ginger using a triangle test showed that the pungency of all the tetraploid types was distinctly higher than that of the diploid ones and that the pungency intensity decreased in the order Kintoki>Oshoga>Sanshu. The pungency intensity of the five samples except for the diploid type of the cultivar Kintoki was correlated with the gingerol content. The antioxidative activity of the methanol extracts from the ginger cultivars used was measured by the weighing, ferric thiocyanate and deoxyribose oxidation methods. Both diploid and tetraploid types displayed the highest antioxidative activity based on the determination by the ferric thiocyanate method. Although the antioxidative activity measured by the deoxyribose oxidation method varied among the diploid and tetraploid types tested, all the tetraploid types inhibited deoxyribose oxidation by 40%.
    These results demonstrated that in the tetraploid types of ginger the gingerol content, antioxidative activity and pungency intensity were higher than in the diploid ones., Jun. 1999, 43, 2, 71-75, 75
  • Refereed, JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, AMER OIL CHEMISTS SOC A O C S PRESS, Antioxidant effect of the constituents of Susabinori (Porphyra yezoensis), R Nakayama; Y Tamura; H Kikuzaki; N Nakatani, The antioxidant activities of several extracts from Susabinori (Porphyra yezoensis) were measured by the ferric thiocyanate method and the thiobarbituric acid method. The methanol, acetone, ethyl acetate, and hexane extracts, and the chloroform-soluble and water-soluble fractions from the chloroform-methanol extract exhibited higher activities than a-tocopherol. The hot water extract showed little activity. Thin-layer chromatography analysis of the active extracts suggested the existence of several antioxidants. The activity of the chloroform soluble fraction was due to chlorophyll analogs. A strong antioxidant was isolated from the methanol extract, accompanied by several amino acids such as leucine and phenylalanine. This compound was identified as usujilene, a kind of mycosporine-glycine like amino acid., May 1999, 76, 5, 649, 653, Scientific journal
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Acetophenone glycosides from thyme (Thymus vulgaris L.), MF Wang; H Kikuzaki; CC Lin; A Kahyaoglu; MT Huang; N Nakatani; CT Ho, Four acetophenone glycosides were isolated from the butanol-soluble fraction of thyme extracts. Their structures were determined by spectral methods (MS, NMR, and 2D-NMR). Among them, two new compounds, 4-hydroxyacetophenone 4-O-[5-O-(3,5-dimethoxy-4-hydroxybenzoyl)-beta-D-apiofuranosyl]-(1-->2)-beta-D-glucopyranoside (1) and 4-hydroxyacetophenone 4-O-[5-O-(4-hydroxybenzoyl)-beta-D-apiofuranosyl]-(1-->2)-beta-D-glucopyranoside (2), were determined. Compound 1 showed weak cytotoxicity, inhibiting DNA synthesis of human leukemia cells., May 1999, 47, 5, 1911, 1914, Scientific journal
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, New bitter diterpenes, rabdosianone I and II, isolated from Isodon japonicus Hara, Y Yamada; N Sako; E Ando; M Yamada; H Kikuzaki; T Yamamoto, The new bitter diterpenes, rabdosianone I(C20H24O5) and II (C22H28O6), were isolated from Isodon japonicus (Japanese name, enmeiso), and their structures were elucidated by spectroscopic methods, Electrophysiological experiments were performed to compare rabdosianone I with quinine. The taste responses of chorda tympani nerves to rabdosianone I were smaller than those to quinine in Wistar rats., Mar. 1999, 63, 3, 524, 529, Scientific journal
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, Spasmolytic activity of aurapten analogs, Y Yamada; M Okamoto; H Kikuzaki; N Nakatani, Seven coumaric compounds analogous to aurapten were synthesized. Their spasmolytic activity against Ba2+, acetylcholine and histamine was evaluated to investigate their structure-activity relationship. The results of the bioassay demonstrated the important roles of the cis type of double bond at C-2' and the epoxide between C-6' and 7'., Apr. 1997, 61, 4, 740, 742, Scientific journal
  • Refereed, J. Home Econ. Jpn., 日本家政学会, Antioxidative Activity of Constituents from Fennel Seeds, KIKUZAKI Hiroe; Reiko Nakayama; Hiroe Kikuzaki; Nobuji Nakatani; Hisashi Horiuchi, The antioxidative activity and active compounds of fennel seeds were studied. The water extract of fennel seeds had a strong inhibition effect on the oxidation of linoleic acid in a water-ethanol solution. Its activity being comparable to that of BHT. The methanol-soluble part of the water extract was fractionated by chromatography on a Sephadex LH-20 column, the strongly antioxidative fractions being purified by repeated chromatography to afford compounds. These compounds were identified as 4-O-β-D-glucosyl sinapyl alcohol, 4, 9-di-O-β-glucosyl alcohol and 4-β-glucosyloxy benzoic acid on the basis of their spectral data. The isolated compounds exhibited the antioxidative effect in comparison with the control., Dec. 1996, 47, 12, 1193-1199, 1199
  • Refereed, 日本味と匂学会誌, 日本味と匂学会, 延命草(Isodon japonicus) に含まれる新規な苦味化合物の構造, KIKUZAKI Hiroe, Dec. 1996, 3, 3, 584-587, S378
  • Refereed, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, AMER CHEMICAL SOC, Stability of anthocyanins of Sambucus canadensis and Sambucus nigra, O Inami; Tamura, I; H Kikuzaki; N Nakatani, Elderberry anthocyanins (Sambucus nigra) including acylated derivatives (Sambucus canadensis) were studied for use as beverage pigments. Cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside-5-O-beta-D-glucopyranoside from S. canadensis was more stable than cyanidin 3-sambubioside from S. nigra. Acylation improved both heat and light stability, whereas glycosidation only stabilized anthocyanins in the presence of light. Cyanidin 3-(E)-p-coumaroyl-sambubioside-5-glucoside changed to three new anthocyanins under light irradiation. These three anthocyanins were isolated, and their structures were identified to be cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside-5-O-beta-D-glucopyranoside, cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside, and cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside., Oct. 1996, 44, 10, 3090, 3096, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, Cyclic diarylheptanoids from rhizomes of Zingiber officinale, H Kikuzaki; N Nakatani, Five new diarylheptanoids were isolated from the rhizomes of Zingiber officinale and their structures elucidated by spectroscopic and chemical methods. They were oxygenated at C-1, 3 and 5 on the heptane chain and cyclized between C-1 and C-5 through oxygen. Copyright (C) Elsevier Science Ltd, Sep. 1996, 43, 1, 273, 277, Scientific journal
  • Refereed, Planta Medica, Georg Thieme Verlag, Secoiridoid glucosides from Swertia mileensis, Hiroe Kikuzaki; Yoko Kawasaki; Seiichi Kitamura; Nobuji Nakatani, Five new acyl secoiridoid glucosides along with three known secoiridoid glucosides, swertiamarin, 2'-O-acetylswertiamarin, and amarogentin, were isolated from the aerial parts of Swertia mileensis. The structures of these compounds were determined to be 2'-O-acetyl-4'-O-trans-feruloylswertiamarin, 2'-O-acetyl-4'-O-cis-feruloylswertiamarin, 2'-O-acetyl-4'-O-trans-p-coumaroylswertiamarin, 2'-O-acetyl-4'-O-cis-p-coumaroylswertiamarin, and 4'-O-trans-p-coumaroylswertiamarin from spectroscopic evidence., 1996, 62, 1, 35, 38, Scientific journal
  • Refereed, Phytochemistry, Acylated anthocyanins from fruits of Sambucus canadensis, Nobuji Nakatani; Hiroe Kikuzaki; Junko Hikida; Mayumi Ohba; Osamu Inami; Itaru Tamura, Two new acylated anthocyanin glycosides were isolated from fruits of Sambucus canadensis. The structures were determined to be cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside)-5-O-β-d-glucopyranoside and cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside. © 1995., 1995, 38, 3, 755, 757, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, LABDANE DITERPENES FROM RHIZOMES OF HEDYCHIUM-CORONARIUM, N NAKATANI; H KIKUZAKI; H YAMAJI; K YOSHIO; C KITORA; K OKADA; WG PADOLINA, Three new labdane diterpenes were isolated together with four known diterpenes [(E)-labda-8(17),12-diene-15,16-dial, coronarin B and D, and isocoronarin D] from rhizomes of Hedychium coronarium. The structures of the new compounds were determined by spectroscopic evidence to be labda-8(17),11,13-trien-15(16)-olide, an ester of labda-8(17), 11,13-trien-15-al-16-oic acid and isocoronarin D, and 7 beta-hydroxycoronarin B., Nov. 1994, 37, 5, 1383, 1388, Scientific journal
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, TRANSFORMATION OF GLYCYRRHIZIC ACID BY ASPERGILLUS SPP, Y YAMADA; A NAKAMURA; K YAMAMOTO; H KIKUZAKI, Glycryrrhizic acid was metabolized to 3-oxo-18 beta-glycyrrhetinic acid via 18 beta-glycyrrhetinic acid by Aspergillus niger, A. oryzae, A. sojae, and A. tamarii. Two methyl esters were derived from these two metabolites and identified by their C-13-NMR spectra and MS data., Feb. 1994, 58, 2, 436, 437
  • Refereed, FOOD PHYTOCHEMICALS FOR CANCER PREVENTION II, AMER CHEMICAL SOC, STRUCTURE OF ANTIOXIDATIVE COMPOUNDS IN GINGER, H KIKUZAKI; Y KAWASAKI; N NAKATANI, Fourteen gingerol related compounds and seven diarylheptanoids were isolated from ginger rhizomes. Their structures were elucidated on the basis of chemical and spectroscopic evidence. Most of the isolated compounds exhibited stronger antioxidative effect than alpha-tocopherol. The antioxidant activity depended upon substituents on the side chain and benzene ring, and the length of side chain., 1994, 547, 237, 243, Scientific journal
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, LC-MS ANALYSIS AND STRUCTURAL DETERMINATION OF NEW AMIDES FROM JAVANESE LONG PEPPER (PIPER-RETROFRACTUM), H KIKUZAKI; M KAWABATA; E ISHIDA; Y AKAZAWA; Y TAKEI; N NAKATANI, Amides in a CH2Cl2 extract from the fruits of Piper retrofractum were detected by HPLC/APCI-MS. Seven new unsaturated amides, together with six known ones, were isolated, and their structures were determined to be N-isobutyl-2E,4E,12Z-octadecatrienamide (1), N-isobutyl-2E,4E,14Z-eicosatrienamide (2), 1-(octadeca-2E,4E,12Z-trienoyl)piperidine (3), 1-(eicosa-2E,4E,14Z-trienoyl)piperidine (4), 1-(octadeca-2E,4E-dienoyl)piperidine (5), 1-(eicosa-2E,4E-dienoyl)piperidine (6), and 1-(eicosa-2E,14Z-dienoyl)piperidine (7) on the basis of chemical and spectroscopic evidence., Aug. 1993, 57, 8, 1329, 1333, Scientific journal
  • Refereed, Journal of Food Science, Antioxidant Effects of Some Ginger Constituents, HIROE KIKUZAKI; NOBUJI NAKATANI, The nonvolatile fraction of the dichloromethane extract of ginger rhizomes exhibited a strong antioxidative activity using linoleic acid as the substrate in ethanol‐phosphate buffer solution. The fraction was purified by chromatographic techniques to provide five gingerol related compounds and eight diarylheptanoids. Among them, 12 compounds exhibited higher activity than α‐tocopherol. The activity was probably dependent upon side chain structures and substitution patterns on the benzene ring. Copyright © 1993, Wiley Blackwell. All rights reserved, 1993, 58, 6, 1407, 1410, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, 2 CURCUMINOID PIGMENTS FROM CURCUMA-DOMESTICA, R NAKAYAMA; Y TAMURA; H YAMANAKA; H KIKUZAKI; N NAKATANI, Two new pigments were isolated from fresh rhizomes of turmeric (Curcuma domestica Val.). Their structures were determined to be 1-(4-hydroxy-3-methoxyphenyl)-7-(3,4-dihydroxyphenyl)-1,6-heptadiene-3,5-dione and 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one by spectroscopic methods., May 1993, 33, 2, 501, 502
  • Refereed, J. Antibact. Antifung. Agents, Identification of Antimicrobial Gingerols from Ginger (Zingiber officinale Roscoe), KIKUZAKI Hiroe; Yasumasa Yamada; Hiroe Kikuzaki; Nobuji Nakatani, Jun. 1992, 20, 309-311, 311
  • Refereed, STARCH-STARKE, VCH PUBLISHERS INC, CHARACTERIZATION OF HIGH-AMYLOSE TYPE ENDOSPERM STARCHES OF RICE PLANTS CULTIVATED IN ASIA, H FUWA; K OKUNO; R ASASHIBA; H KIKUZAKI; M ASAOKA; N INOUCHI; Y SUGIMOTO, Starch granules were prepared from 14 indica rice grains which were collected from various places of Asia and had the amylose content of 28% and over by a blue value method. The starches were high- and medium-amylose types by the enzymatic and chromatographic method. However, none of them belonged to the type of high amylose mutants which were induced by the chemical mutagen treatment of a japonica rice cultivar: Kinmaze and possessed similar characteristics of endosperm starch to that of the amylose-extender (ae) mutant of maize (Zea mays L.)., Jun. 1992, 44, 6, 203, 205, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, CONSTITUENTS OF ZINGIBERACEAE .5. GINGERDIOL RELATED-COMPOUNDS FROM THE RHIZOMES OF ZINGIBER-OFFICINALE, H KIKUZAKI; SM TSAI; N NAKATANI, [6]-Gingerdiol and four analogues, (3R,5S)-5-acetoxy-3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decane, (3R,5S)-3-acetoxy-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decane,(3R,5S)-3,5-diacetoxy-1-(4-hydroxy-3-methoxyphenyl)decane and (3R,5S)-3,5-diacetoxy-1-(3,4-dimethoxyphenyl)decane, were isolated from the rhizomes of Zingiber officinale. Their structures were established by chemical and spectroscopic evidence., May 1992, 31, 5, 1783, 1786, Scientific journal
  • Refereed, Chemistry Express, New Phenolic Compounds of Ginger (Zingiber officinale Roscoe), KIKUZAKI Hiroe; Nobuji Nakatani; Hiroe Kikuzaki, Mar. 1992, 7, 221-224
  • Refereed, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, MICROBIAL TRANSFORMATION OF (+/-)-EPOXYAURAPTEN BY PSEUDOMONAS-AERUGINOSA, Y YAMADA; H KIKUZAKI; N NAKATANI, Jan. 1992, 56, 1, 153, 154
  • Refereed, Chemistry Express, Constituents of Gyrophora esculenta, KIKUZAKI Hiroe; Nobuji Nakatani; Chon Ho Kim; Hiroe Kikuzaki; Teruyo Muroi; Yoko Matsumura, Aug. 1991, 6, 587-590
  • Refereed, CHEMICAL & PHARMACEUTICAL BULLETIN, PHARMACEUTICAL SOC JAPAN, CONSTITUENTS OF ZINGIBERACEAE .1. DIARYLHEPTANOIDS FROM THE RHIZOMES OF GINGER (ZINGIBER-OFFICINALE ROSCOE), H KIKUZAKI; J USUGUCHI; N NAKATANI, Two new diarylheptanoids, meso-3,5-diacetoxy-1,7-bis(4-hydroxy-3-methoxyphenyl)heptane (3a) and 3,5-diacetoxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)heptane (4a) were isolated from the rhizomes of ginger (Zingiber officinale ROSCOE), together with dehydroxytetrahydrocurcumin (gingerenone A, 1) and hexahydrocurcumin (2). Their structures were elucidated on the basis of chemical and spectroscopic evidence., Jan. 1991, 39, 1, 120, 122, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, CONSTITUENTS OF PIPERACEAE .4. ANTIMICROBIAL PHENYLPROPANOIDS FROM PIPER-SARMENTOSUM, T MASUDA; A INAZUMI; Y YAMADA; WG PADOLINA; H KIKUZAKI; N NAKATANI, One new and three known phenylpropanoids have been isolated from the leaves of Piper sarmentosum. They are 1-allyl-2,6-dimethoxy-3,4-methylenedioxybenzene, 1-allyl-2,4,5-trimethoxybenzene, 1-(1-E-propenyl)-2,4,5-trimethoxybenzene and 1-allyl-2-methoxy-4,5-methylenedioxybenzene. The new compound showed antimicrobial activity against Escherichia coli and Bacillus subtilis., 1991, 30, 10, 3227, 3228, Scientific journal
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, CONSTITUENTS OF ZINGIBERACEAE .4. DIARYLHEPTANOIDS FROM RHIZOMES OF ZINGIBER-OFFICINALE, H KIKUZAKI; M KOBAYASHI; N NAKATANI, Six new diarylheptanoids, 5-hydroxy-7-(4-hydroxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-3-heptanone, 3,5-diacetoxy-7-(3,4-dihydroxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)heptane, 5-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-3-heptanone, 5-hydroxy-7-(4-hydroxy-3,5-dimethoxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-3-heptanone, (3R,5S)-3,5-dihydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)heptane and (3S,5S)-3,5-diacetoxy-1,7-bis(3,4-dihydroxyphenyl)heptane were isolated from the rhizomes of Zingiber officinale, together with two known diarylheptanoids. Their structures were established by chemical and spectroscopic evidences., 1991, 30, 11, 3647, 3651, Scientific journal
  • Refereed, Chemistry Express, Synthesis of Capsaicinol [1], KIKUZAKI Hiroe; Toshiya Masuda; Hiroe Kikuzaki; Yukari Tachibana; Nobuji Nakatani, Jun. 1990, 5, 369-372, 372
  • Refereed, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC BIOSCI BIOTECHN AGROCHEM, STRUCTURE OF A NEW ANTIOXIDATIVE PHENOLIC-ACID FROM OREGANO (ORIGANUM-VULGARE L), H KIKUZAKI; N NAKATANI, Feb. 1989, 53, 2, 519, 524, Scientific journal
  • Refereed, Medical, Biochemical and Chemical Aspects of Free Radicals, Elsevier, Antioxidative Compounds from Edible Plants: Phenolic Amides from Capsicum frutescens L., KIKUZAKI Hiroe; Nobuji Nakatani; Yukari Tachibana; Hiroe Kikuzaki, 1989, 453-456, 456
  • Refereed, PHYTOCHEMISTRY, PERGAMON-ELSEVIER SCIENCE LTD, LIGNANS OF PAPUAN MACE (MYRISTICA-ARGENTEA WARB) .2. DIARYLDIMETHYLBUTANE LIGNANS FROM MYRISTICA-ARGENTEA AND THEIR ANTIMICROBIAL ACTION AGAINST STREPTOCOCCUS MUTANS, N NAKATANI; K IKEDA; H KIKUZAKI; M KIDO; Y YAMAGUCHI, 1988, 27, 10, 3127, 3129, Scientific journal
  • Refereed, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC BIOSCI BIOTECHN AGROCHEM, A NEW ANTIOXIDATIVE GLUCOSIDE ISOLATED FROM OREGANO (ORIGANUM-VULGARE L), N NAKATANI; H KIKUZAKI, Oct. 1987, 51, 10, 2727, 2732, Scientific journal
  • Refereed, Chemistry Express, Structure of Diacylated Anthocyanins from Red Cabbage (Brassica oleracea), KIKUZAKI Hiroe; Nobuji Nakatani; Kayo Ikeda; Mikio Nakamura; Hiroe Kikuzaki, Sep. 1987, 2, 555-558, 558
  • Refereed, Chemistry Express, Structure of Two Acylated Anthocyanins from Red Cabbage (Brassica oleracea), KIKUZAKI Hiroe; Kayo Ikeda; Hiroe Kikuzaki; Mikio Nakamura; Nobuji Nakatani, Sep. 1987, 2, 563-366, 566
  • Refereed, Eiyo To Shokuryo, Japan Society of Nutrition and Food Science, 大豆油の消化管内移動におよぼすペクチンの影響, KIKUZAKI Hiroe, The transit of soybean oil through the gastrointestinal tract was studied in rats fed 4% pectin diet in comparision with those fed 4% cellulose diet. The structural changes of the surface of the small intestinal mucosa were also observed by scanning electron microscopy.
    1. The ratio of lipid content in the feces of rats fed 4% pectin diet was higher than that of rats fed 4% cellulose diet.
    2. The apparent lipid ahsorption ratio of rats fed 4% pectin diet was lower than that of rats fed 4% cellulose diet.
    3. Rats fed 4% pectin diet showed irregular arrangement of the small intestinal villi, especially abnormal structure at the top of the villi., Jan. 1987, 40, 1, 64-69, 69
  • Refereed, J. Biol. Macromol., Japan Science Society of Biological Macromolecules, Isolation of phenolic components from strawberry cultivar 'Tokun' and their inhibitory activities on recombinant human histidine decarboxylase, Yoko Nitta; Miyuki Mori; Yuji Noguchi; Yuichi Uno; Misaki Ishibashi; Hiroshi Ueno; Hiroe Kikuzaki, Aug. 2020, 20, 2, 33, 39, Scientific journal
  • Refereed, Bioscience, biotechnology, and biochemistry, The ellagitannin trimer rugosin G inhibits recombinant human histidine decarboxylase., Yoko Nitta; Hideyuki Ito; Hirohumi Komori; Hiroshi Ueno; Daiki Takeshima; Mikiko Ito; Motoyoshi Sakaue; Hiroe Kikuzaki, Rugosin G, an ellagitannin trimer, was isolated from the water-soluble fraction of red rose petals, and its inhibitory activity against recombinant human histidine decarboxylase was investigated. Rugosin G showed potent inhibition compared to ellagitannin monomers and a dimer with macrocyclic structure (oenothein B), suggesting the potent inhibition of rugosin G was attributed to its linear oligomeric conformation. Abbreviations: HDC, histidine decarboxylase; Me2CO, acetone; EtOAc, ethyl acetate., Jul. 2019, 83, 7, 1315, 1318, Scientific journal, True
  • Refereed, Food Science and Technology Research, Japanese Society for Food Science and Technology, Components for Inhibiting Lipid Oxidation Related to Discoloration of Carotenoid Contained in Sugarcane Extract, Hiroe Kikuzaki; Mihoko Masuda; Eri Kishi; Mina Ozaki; Kanako Kondo; Aya Kanai; Kazuyo Shiomi; Toma Furuta; Masami Mizu; Yukie Nagai, 2019, 25, 5, 715, 725, Scientific journal
  • Refereed, NATURAL PRODUCT COMMUNICATIONS, NATURAL PRODUCTS INC, Biological Activities of Isolated Compounds from Three Edible Malaysian Red Seaweeds, Gracilaria changii, G. manilaensis and Gracilaria sp., Yosie Andriani; Desy Fitrya Syamsumir; Tee Ching Yee; Faizah Shaharom Harisson; Gan Ming Herng; Siti Aishah Abdullah Christine Abellana Oroscoa; Christine Abellana Orosco; Abdul Manaf Ali; Jalifah Latip; Hiroe Kikuzaki; Habsah Mohamad, Gracilaria species are red marine macroalgae that are found abundantly in Malaysia. Gracilaria changii from Morib, Selangor, G. manilaensis and Gracilaria sp. from Gelang Patah, Johor were used in this study. Five compounds were successfully isolated and identified as hexadecanoic acid (1), cholest-5-en-3-ol (2), 2-hydroxymyristic acid (3), cholesteryl myristate (4) and 1-(4'-methoxyphenyl)-3-(2 '',4 '',6 ''-trihydroxyphenyl)-3-hydroxypropanone (5) based on spectral data analysis (IR, UV, GC-MS, H-1 NMR, C-13 NMR, HMQC and HMBC). All compounds isolated were tested for cytotoxicity (MTT assay for HL-60 and MCF-7 cell lines), and antibacterial (disc diffusion method), antioxidant (DPPH free radical scavenging assay and xanthine oxidase inhibitory assay) and acetylcholinesterase inhibitory (AChE) activity (TLC bioautographic method). Compounds 1 and 3 exhibited strong cytotoxic activity against HL-60 and MCF-7 cell lines. Compound 5 showed high antioxidant activity in both the DPPH free radical scavenging and xanthine oxidase inhibition assays. Compound 1 showed positive activity for AChE inhibitory with a minimum inhibition dose of 0.625 mu g sample. All compounds demonstrated antibacterial activity producing 8 to 14 mm inhibition zones. A positive control was applied to all bioassays and experiments were performed with three replicates. Results demonstrated that three edible red seaweeds are rich sources of bioactive compounds with potential application for pharmaceutical purposes., Aug. 2016, 11, 8, 1117, 1120, Scientific journal
  • Refereed, ORIENTAL FOODS AND HERBS, AMER CHEMICAL SOC, Antioxidants from some tropical spices, H Kikuzaki, 2003, 859, 176, 189, Scientific journal
  • 日本栄養・食糧学会大会講演要旨集, (公社)日本栄養・食糧学会, 葛デンプン晒し液より単離した化合物の化学構造解析およびエストロゲン様活性の評価, 上山 蓮実; 菊崎 泰枝; 北爪 亮太; 餅田 尚子; 松村 羊子; 栢野 新市, Apr. 2019, 73回, 263, 263
  • 日本栄養・食糧学会大会講演要旨集, (公社)日本栄養・食糧学会, 葛デンプン晒し液より単離したイソフラボンのエストロゲン様活性及び抗変異原性の評価, 栢野 新市; 土田 真由香; 柳澤 尚子; 松村 羊子; 菊崎 泰枝; 北田 善三, Apr. 2011, 65回, 159, 159
  • Abstracts of the Annual Meeting of the Japan Society of Cookery Science, The Japan Society of Cookery Science, Antimicrobial effects of prune extract (concentrated prune juice) added to cooked rice on food-borne pathogenic bacteria, Tsuruta Yumi; Kayano Shin-ichi; Ikami Takao; Kikuzaki Hiroe; Nakatani Nobuji; Nishikawa Yoshikazu, 【目的】プルーンは、抗酸化性など優れた機能性を有する果実であり、そのエキスは、健康食品として広く用いられている。料理にも利用し易く、様々な調理法によって摂取されているが、特に、プルーンエキスを米飯と混ぜる調理法は、味や栄養性のみならず、米飯の保存性を高めると言われている。プルーンは、抗菌性を示すフェノール性化合物および有機酸を含んでおり、これらの成分は米飯の保存性を向上していると考えられる。本研究は、プルーンエキスの添加が米飯中における食中毒菌の増殖に及ぼす影響を検証することを目的とした。
    【方法】米飯における食中毒の代表的な菌である黄色ブドウ球菌およびセレウス菌を供試菌として用いた。試験米飯には、プルーンエキス5% (w/w)、10%添加群、食酢添加(酢飯)群および無添加群を設け、供試菌およびセレウス菌芽胞液を103から104CFU/gとなるように接種した。25℃および37℃で保存後、経時的(8から48時間)に米飯中の菌数を測定した。
    【結果】試験米飯のpHは、無添加群で約6.5、5%プルーンエキス添加群で約4.8、10%添加群で約4.5、食酢添加群で約4.2であった。黄色ブドウ球菌、セレウス菌およびセレウス菌芽胞接種米飯のいずれにおいても、プルーンエキス5%添加によって、供試菌の増殖が無添加群に比べて著しく低下した。10%添加群では菌の増殖が抑制され、芽胞の発芽抑制も48時間まで持続した。また、プルーンエキス10%添加群と酢飯の増殖抑制効果を比較したところ、両者ともに強い増殖抑制および芽胞の発芽抑制を示した。したがって、米飯にプルーンエキスを添加する調理法は、酢飯と同等の抗菌性を有し、食中毒の予防に有効であることが示唆された。, 2004, 16, 7, 7
  • Refereed, Antioxidants, MDPI AG, Antioxidant Potential of Non-Extractable Fractions of Dried Persimmon (Diospyros kaki Thunb.) in Streptozotocin-Induced Diabetic Rats, Naoko Mochida; Yoko Matsumura; Masahiro Kitabatake; Toshihiro Ito; Shin-ichi Kayano; Hiroe Kikuzaki, Oxidative stress causes the progression of diabetes and its complications; thus, maintaining the balance between reactive oxygen species produced by hyperglycemia and the antioxidant defense system is important. We herein examined the antioxidant potential of non-extractable fractions of dried persimmon (NEP) against oxidative stress in diabetic rats. Rats with streptozotocin-induced type 1 diabetes (50 mg/kg body weight) were administered NEP for 9 weeks. Antioxidant enzyme activities and concentration of antioxidants in liver tissues were analyzed with a microplate reader. Extensor digitorum longus (EDL) and soleus muscle fibers were stained with succinate dehydrogenase and muscle fiber sizes were measured. The administration of NEP increased the body weight of diabetes rats. Regarding antioxidant activities, the oxygen radical absorbance capacity and superoxide dismutase activity in liver tissues significantly increased. In addition, increases in glutathione peroxidase activity in liver tissues and reductions in the cross-sectional area of EDL muscle fibers were significantly suppressed. In these results, NEP improved the antioxidant defense system in the liver tissues of diabetic rats, in addition to attenuating of muscle fibers atrophy against oxidative damage induced by hyperglycemia., 11 Aug. 2022, 11, 8, 1555, 1555, Scientific journal
  • Refereed, Fitoterapia, Elsevier BV, Identification of components in coriander (Coriandrum sativum L.) inhibiting degranulation of RBL-2H3 cells, Rika Ohara; Takuya Sugahara; Yuna Sugie; Hiroyuki Onda; Nanami Yoshino; Kosuke Nishi; Momoko Ishida; Hiroe Kikuzaki, Nov. 2022, 163, 105298, 105298, Scientific journal
  • Refereed, Molecules, MDPI AG, Umbelliferose Isolated from Cuminum cyminum L. Seeds Inhibits Antigen-Induced Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells, Momoko Ishida; Rika Ohara; Fuka Miyagawa; Hiroe Kikuzaki; Kosuke Nishi; Hiroyuki Onda; Nanami Yoshino; Takuya Sugahara, Cuminum cyminum L. (cumin) is an annual plant of the Umbelliferae family native to Egypt. We previously showed that the aqueous extract of cumin seeds suppresses degranulation by downregulating the activation of antigen-induced intracellular signaling molecules in rat basophilic leukemia RBL-2H3 cells. However, the active substances in the extract have not yet been identified. Accordingly, herein, we aimed to ascertain the water-soluble substances present in cumin seeds that inhibit degranulation, which led to the identification of umbelliferose, a characteristic trisaccharide present in plants of the Umbelliferae family. Our study is the first to reveal the degranulation-suppressing activity of umbelliferose, and quantification studies suggest that cumin seed powder contains 1.6% umbelliferose. Raffinose, an isomer of umbelliferose, was also found to significantly suppress antigen-induced degranulation, but less so than umbelliferose. Both umbelliferose and raffinose contain sucrose subunits in their structures, with galactose moieties bound at different sites. These differences in structure suggest that the binding of galactose to the sucrose subunit at the α1-2 bond contributes to its strong degranulation-inhibiting properties., 25 Jun. 2022, 27, 13, 4101, 4101, Scientific journal

MISC

  • Not Refereed, アレルギーの臨床, 薬用植物を使用したアレルギー様食中毒予防の研究, 新田陽子; 菊﨑泰枝, 30 Dec. 2020, 40, 14, 50, 52, Introduction scientific journal
  • Not Refereed, Food & Food Ingredients Journal of Japan, Recent Studies on the Application of Data for Food Components Based on Mass Spectrometry, 菊﨑泰枝, Oct. 2020, 225, 4, 339, 345, Introduction other
  • Not Refereed, Functional Food, フトモモ科スパイス(クローブ,オールスパイス)の食文化と機能性, KIKUZAKI Hiroe, Dec. 2017, 11, 2, 111-116
  • Not Refereed, Functional Food, ショウガの食文化と機能性, KIKUZAKI Hiroe, Dec. 2017, 11, 2, 122-128
  • Not Refereed, AROMA RESEARCH, フレグランスジャーナル社, ショウガ科香辛料の特徴的成分~その機能性~, KIKUZAKI Hiroe, Aug. 2017, 18, 3, 42-47, 247
  • Not Refereed, ビタミン, ガロタンニン,エラジタンニンによるヒスチジン脱炭酸酵素の活性阻害の解析, 森美幸; 菊崎泰枝; 植野洋志; 小森博文; 新田陽子, 25 Apr. 2016, 90, 4, 184
  • Not Refereed, 生物高分子, ローズペタル成分によるヒト由来ヒスチジン脱炭酸酵素の阻害, 森美幸; 渡邉裕香; 新田陽子; 植野洋志; 秋本梢; 小林慧子; 菊崎泰枝, 01 Sep. 2015, 15, 1, 74
  • Not Refereed, 生物高分子, メドウスイート抽出液によるサバのヒスタミン汚染抑制, 安方芙実子; 北元憲利; 新田陽子; 江戸良也子; 植野洋志; 菊崎泰枝, 01 Sep. 2015, 15, 1, 74
  • Not Refereed, 日本農芸化学会大会講演要旨集(Web), Rosa gallicaの花弁に含まれるヒスチジン脱炭酸酵素阻害成分の探索, 秋本梢; 渡邉裕香; 新田陽子; 植野洋志; 小林慧子; 菊崎泰枝, 05 Mar. 2014, 2014, 4B04A08 (WEB ONLY)
  • Not Refereed, 生物高分子, ヒスタミン合成を阻害する薬用植物成分の探索, 胡榴; 新田陽子; 菊崎泰枝; 植野洋志, 01 Oct. 2008, 8, 3, 87
  • Not Refereed, Journal of Clinical Biochemistry and Nutrition, Antimicrobial Activity of Saponins from Leaves of Bacopa monnieri, Masashi Hisamoto; Naomi Nishihara; Tohru Okuda,Huong Lai Mai; Hiroe Kikuzaki; Koki Yokotsuka; Koki Yokotsuka, 2008, 43, 1, 3
  • Not Refereed, 日本農芸化学会関西支部講演会講演要旨集, ヒト由来ヒスチジンデカルボキシラーゼの活性を阻害する香辛料成分, 新田陽子; 菊崎泰枝; 植野洋志, 2008, 455th, 16
  • Not Refereed, 香料, 日本香料協会, ショウガ科植物の化学成分, KIKUZAKI Hiroe, Mar. 2007, 233, 233, 81-87, 87
  • Not Refereed, 生物高分子, ヒスチジン脱炭酸酵素の活性を阻害する食品成分, 新田陽子; 菊崎泰枝; 植野洋志, 01 Jul. 2006, 6, 2, 57
  • Not Refereed, 香料, オールスパイス(ピメント)の抗酸化成分の解明, KIKUZAKI Hiroe, Oct. 2002, 215, 101-106
  • Not Refereed, FOOD Style 21, 食品化学新聞社, 沖縄の伝統的食用植物の機能性, KIKUZAKI Hiroe, Jul. 2001, 5, 7, 50-53, 53
  • Not Refereed, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, Antioxidants in the ginger family., N Nakatani; H Kikuzaki, Aug. 2000, 220, U42, U42, Summary international conference
  • Not Refereed, NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, JAPAN SOC BIOSCI BIOTECHN AGROCHEM, ANTIOXIDANT ACTIVITY OF POLYPHENOLS IN FOOD, N NAKATANI; H KIKUZAKI, Sep. 1995, 69, 9, 1189, 1192, Book review
  • Not Refereed, 日本農芸化学会誌, 食品中のポリフェノールの抗酸化活性, KIKUZAKI Hiroe, Sep. 1995, 69, 9, 1189-1192
  • Not Refereed, フードケミカル, 香辛植物の抗酸化物質とその生体内酸化障害抑制作用, KIKUZAKI Hiroe, Mar. 1995, 3, 49-53
  • Not Refereed, 食品工業, スパイス・ハーブの化学と機能, KIKUZAKI Hiroe, Oct. 1994, 37, 19, 57-65, 65
  • Not Refereed, aromatopia, ハーブおよびその精油の抗菌作用と食品への応用, KIKUZAKI Hiroe, Sep. 1994, 9, 3, 42-46, 46
  • Not Refereed, aromatopia, ローズマリーの抗菌成分とその活性, KIKUZAKI Hiroe, Nov. 1993, 3, 4, 40-42, 37
  • Not Refereed, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, STRUCTURE OF ANTIOXIDATIVE COMPOUNDS OF GINGER, H KIKUZAKI; Y KAWASAKI; N NAKATANI, Aug. 1992, 204, 139, AGFD, Summary international conference
  • Not Refereed, 食品と開発, 香辛料成分の構造特性と食品劣化抑制機能, KIKUZAKI Hiroe, Mar. 1992, 27, 3, 7-10, 10
  • Not Refereed, Chemistry Express, Structure of Iridoids from Swertia mileensis He et Shi, KIKUZAKI Hiroe; Hiroe Kikuzaki; Seiichi Kitamura; Nobuji Nakatani, Dec. 1988, 3, 751-754
  • Not Refereed, フードケミカル, ナツメグ、メースの抗菌・抗酸化作用, KIKUZAKI Hiroe, Feb. 1988, 2, 70-74
  • ビタミン, 日本ビタミン学会, 15. 上気道病原菌に対する甘草の抗菌化合物, 田中 康雄; 菊崎 泰枝; 福田 清司; 中谷 延二, 25 Aug. 2001, 75, 8, 448, 448
  • ビタミン, 日本ビタミン学会, 13. Greater cardamom(Amomum subulatum)から単離されたDPPHラジカル捕捉活性化合物, 菊崎 泰枝; 河合 弥生; 中谷 延二, 25 Jul. 2001, 75, 7, 404, 404
  • 日本農藝化學會誌, 社団法人日本農芸化学会, ショウガ(zingiber officinale Roscoe)の成分研究(9) : 新規環状ジアリールヘプタノイドの構造 : 物理化学・分析化学, 菊崎 泰枝; 中谷 延二, 05 Jul. 1995, 69, 50, 50

Books etc

  • 978-4-7813-1521-8
  • 新スタンダード栄養・食物シリーズ5 食品学-食品成分と機能性-, 東京化学同人, KIKUZAKI Hiroe, 分担, Apr. 2016, 79-98, 107-108, 253-262, Not Refereed, 9784807916658
  • 栄養科学イラストレイテッド 食品学II 食品の分類と特性、加工を学ぶ, 羊土社, KIKUZAKI Hiroe, 分担, Mar. 2016, 148-154, Not Refereed, 9784758108805
  • 管理栄養士のための経営管理 アメリカにおける考え方と実践を学ぶ, 東京教学社, KIKUZAKI Hiroe, 分担, Dec. 2015, 522-545, Not Refereed, 9784808260439
  • 栄養科学イラストレイテッド 食品学I 食品の成分と機能を学ぶ, 羊土社, KIKUZAKI Hiroe, 編集, Nov. 2015, 90-91, 100-122, Not Refereed, 9784758108799
  • 新訂 食健康科学, 放送大学教育振興会, KIKUZAKI Hiroe, 分担, Mar. 2015, 27-62, 102-122, Not Refereed, 9784595140471
  • Development & Market of Antioxidant, シーエムシー出版, KIKUZAKI Hiroe, 分担, May 2014, 47-62, Not Refereed
  • 生食のおいしさとリスク, エヌ・ティー・エス, KIKUZAKI Hiroe, 分担, Jun. 2013, 459-466, Not Refereed, 9784864690669
  • 改訂版 食と健康, 放送大学教育振興会, KIKUZAKI Hiroe, 分担, Mar. 2012, 28-57, 103-119, Not Refereed, 9784595313493
  • スパイスなんでも小事典-おいしくて体によい使い方, 講談社ブルーバックス, KIKUZAKI Hiroe, 分担, Sep. 2011, 57-59, 81-83, 164-168, Not Refereed, 9784062576987
  • 栄養科学ファウンデーションシリーズ 給食経営管理論, 朝倉書店, KIKUZAKI Hiroe, 分担, Mar. 2011, 1-4, Not Refereed
  • 新版 食べ物と健康[食品学総論], 八千代出版, KIKUZAKI Hiroe, 分担, Feb. 2011, 7-30, 55-75, Not Refereed
  • 新しい食品加工学, 南江堂, KIKUZAKI Hiroe, 分担, Jan. 2011, 135-137, Not Refereed
  • Spices and Herbs-Food Functions and Application Technics, シーエムシー出版, KIKUZAKI Hiroe, 編集, Jan. 2011, 42-44、50-56、85-92、127-135, Not Refereed
  • 健康と栄養のための有機化学, 建帛社, KIKUZAKI Hiroe, 分担, Apr. 2010, 138-165, Not Refereed
  • PDCAに基づく給食経営管理実習, 化学同人, KIKUZAKI Hiroe, 分担, Apr. 2009, 13-14, 31-36, Not Refereed
  • 食健康科学, 放送大学教育振興会, KIKUZAKI Hiroe, 分担, Mar. 2009, 101-148,254-265, Not Refereed
  • スタンダード栄養・食物シリーズ5 食品学-食品成分と機能性 第2版, 東京化学同人, KIKUZAKI Hiroe, 分担, Sep. 2008, 76-96, 104-105, 257-265, Not Refereed
  • 食品機能性の科学, KIKUZAKI Hiroe, 分担, Apr. 2008, 55-59、1042-1045, Not Refereed
  • 栄養・食糧学用語辞典, 建帛社, KIKUZAKI Hiroe, 分担, Oct. 2007, Not Refereed
  • 管理栄養士コースで学ぶ?キャリアデザインのために?, 同文書院, KIKUZAKI Hiroe, 分担, Apr. 2007, 60-62, Not Refereed
  • 食品学実験, 光生館, KIKUZAKI Hiroe, 編集, Apr. 2007, 87-113, 148-153, Not Refereed
  • 基礎調理実習 食品・栄養・大量調理へのアプローチ, 化学同人, KIKUZAKI Hiroe, 分担, Mar. 2007, 65-83, Not Refereed
  • 食品と技術・「大学の研究室-大阪市立大学大学院生活科学研究科食・健康科学講座菊﨑研究室-」, 食品産業センター, KIKUZAKI Hiroe, 筆頭著者, Jan. 2007, 10-13, Not Refereed
  • 2007管理栄養士国家試験新全科の要点, 医歯薬出版, KIKUZAKI Hiroe, 分担, Aug. 2006, 154-161, 428-429, Not Refereed
  • 食と健康-食品の成分と機能, 放送大学教育振興会, KIKUZAKI Hiroe, 分担, Mar. 2006, 95-107,215-227, Not Refereed
  • 栄養・食糧学データハンドブック, 同文書院, KIKUZAKI Hiroe, 分担, Jan. 2006, 191-193, Not Refereed
  • 平成17年度大阪府栄養教諭免許法認定講習テキスト-学校栄養教育の理論と実践・栄養教育実習事前指導, KIKUZAKI Hiroe, 分担, Aug. 2005, 41-50, Not Refereed
  • 2006新カリ対応管理栄養士国家試験完全攻略○×チェック実戦4000問, 医歯薬出版, KIKUZAKI Hiroe, 分担, Jul. 2005, 64-66、275-278, Not Refereed
  • 生活科学I 食の科学, 放送大学教育振興会, KIKUZAKI Hiroe, 分担, Apr. 2005, 111-128, 200-220, 278-293, Not Refereed
  • 読売ファミリー北版8月4日号・疑問解決隊-昆布はなぜ海の中でだしを出さないの, 読売ファミリー編集部, KIKUZAKI Hiroe, 監修, Aug. 2004, Not Refereed
  • わたしの食卓3・ハーブとスパイス、本当の使い方を知っていますか?, 毎日新聞社, KIKUZAKI Hiroe, 監修, Aug. 2004, 12-15、24-25、34-35, Not Refereed
  • 新版 家政学事典, 朝倉書店, KIKUZAKI Hiroe, 分担, Jul. 2004, 430, Not Refereed
  • 食生活研究・野菜の品質特性の変遷と食生活との関り(2), 食生活研究会, KIKUZAKI Hiroe, 分担, Jul. 2004, 14-21, Not Refereed
  • 食生活研究・野菜の品質特性の変遷と食生活との関り(1), 食生活研究会, KIKUZAKI Hiroe, 分担, May 2004, 13-22, Not Refereed
  • 食べ物と健康 調理学, 医歯薬出版, KIKUZAKI Hiroe, 分担, Feb. 2004, 55-56、180-188、205-210、256-259, Not Refereed
  • Oriental Foods and Herbs, Chemistry and Health Effects, American Chemical Society, KIKUZAKI Hiroe; H. Kikuzaki; authors, 分担, Sep. 2003, 176-189, Not Refereed
  • 栄養科学シリーズNEXT-調理学実習, 講談社, KIKUZAKI Hiroe, 分担, Apr. 2003, Not Refereed
  • スタンダード栄養・食物シリーズ5 食品学-食品成分と機能性, 東京化学同人, KIKUZAKI Hiroe, 分担, Apr. 2003, 70-90、98-100、252-256, Not Refereed
  • きょうどう・お料理サイエンス, コープこうべ, KIKUZAKI Hiroe, 監修, Apr. 2003, 4-5, Not Refereed
  • 食と健康-情報のウラを読む, 丸善, KIKUZAKI Hiroe, 分担, Nov. 2002, 173-198, Not Refereed
  • 食品学実験書, 医歯薬出版, KIKUZAKI Hiroe, 分担, Aug. 2002, 108-116、132-135、191-196, Not Refereed
  • 栄養と健康の専門知識がよくわかる食生活, 全衛連「食生活」出版局, KIKUZAKI Hiroe, 分担, Nov. 2001, 79-83, Not Refereed
  • Quality Management of Nutraceuticals, American Chemical Society, KIKUZAKI Hiroe; P.A. Lachance; authors, 分担, Jan. 2001, 230-240, Not Refereed
  • AERA Mook-食生活学がわかる, 朝日新聞社, KIKUZAKI Hiroe, 分担, Nov. 2000, 132-135, Not Refereed
  • Herbs, Botanicals & Teas, Technomic Publishing Co. Inc., KIKUZAKI Hiroe; M. Atha, 分担, Jun. 2000, 75-105, Not Refereed
  • 生活科学最前線90のトピック, 中央法規出版, KIKUZAKI Hiroe, 分担, Sep. 1999, 48-49, Not Refereed
  • 抗酸化成分のすべて, 先端医学社, KIKUZAKI Hiroe, 分担, Sep. 1998, 148-156, Not Refereed
  • 食品総合辞典, 丸善, KIKUZAKI Hiroe, 分担, Mar. 1998, Not Refereed
  • 栄養学ハンドブック(第3版), 技報堂出版, KIKUZAKI Hiroe, 分担, Nov. 1996, 152-154, Not Refereed
  • 活性酸素と医食同源, 共立出版, KIKUZAKI Hiroe, 分担, Jul. 1996, 216-219, Not Refereed
  • 昭和63年度健康情報調査報告書 香辛料, 健康・体力づくり事業財団, KIKUZAKI Hiroe, 分担, Mar. 1989, 364-391, Not Refereed
  • 食品学 : 食品成分と機能性, 東京化学同人, 久保田, 紀久枝; 森光, 康次郎, 第II部 食品の成分 10 嗜好成分-色素成分、呈味成分、注目されている嗜好成分の機能性, Mar. 2021, x, 292p, 9784807916832, cinii_books
  • スパイス・ハーブの機能と応用, シーエムシー出版, 森川, 敏生, 第4章4 フトモモ科(オールスパイス、クローブ), pp.121-130, Oct. 2020, vii, 353p, 9784781315218, cinii_books
  • 9784807916832
  • 978-4-524-22851-5
  • 978-4-7581-1366-3
  • 978-4-7581-1365-6
  • 新しい食品加工学 改訂第3版, 南江堂, 菊﨑泰枝; 高村仁知他, 第4章 食品の加工-香辛料 140~142, 25 Mar. 2022, 9784524228515
  • 食品の成分と機能を学ぶ, 羊土社, 水品, 善之; 菊崎, 泰枝; 小西, 洋太郎, 第3章 食品の二次機能(嗜好成分の化学)99~123, 01 Nov. 2021, 211p, 9784758113656, cinii_books
  • 食品の分類と特性、加工を学ぶ, 羊土社, 栢野, 新市; 水品, 善之; 小西; 洋太郎; 菊﨑泰枝, 第5章 調味料類、香辛料類、嗜好飲料類 156~162, 01 Nov. 2021, 227p, 9784758113663, cinii_books

Presentations

  • 葛西円; 菊﨑泰枝; 新田陽子, 第466回ビタミンB研究協議会, エラジタンニン、ガロタンニンによる食品中ヒスタミン蓄積抑制機構の解明, Oral presentation, 05 Mar. 2022, 05 Mar. 2022, 05 Mar. 2022, rm:research_project_id
  • Rica Ohara; Yuna Sugie; Momoko Ishida; Takuya Sugahara; Hiroyuki Onda; Nanami Yoshino; Hiroe Kikuzaki, Pacifichem 2021, Elucidation of components in coriander leaves and their functions, Poster presentation, 19 Dec. 2021, 16 Dec. 2021, 21 Dec. 2021
  • 葛西円; 菊﨑泰枝; 新田陽子, 日本生物高分子学会2021年度大会, エラジタンニン、ガロタンニンによるモルガン菌由来ヒスチジン脱炭酸酵素の阻害, Oral presentation, 18 Dec. 2021, 18 Dec. 2021, 18 Dec. 2021, rm:research_project_id
  • 竹元優花; 小原理加; 高砂美有; 新田陽子; 小倉裕範; 菊﨑泰枝, 第35回日本香辛料研究会, メドウスイートに含まれるヒスタミン産生抑制成分の探索, Oral presentation, 04 Dec. 2021, 04 Dec. 2021, 04 Dec. 2021, rm:research_project_id
  • 和田あゆみ; 山下慶子; 熊崎ほなみ; 新田陽子; 小倉裕範; 菊﨑泰枝, 第26回日本フードファクター学会学術集会, クローブに含まれるヒスタミン生成抑制成分の探索, Poster presentation, 20 Nov. 2021, 20 Nov. 2021, 21 Nov. 2021, rm:research_project_id
  • 小野智幹; 石田萌子; 西甲介; 小原理加; 菊﨑泰枝; 吉野七海; 菅原卓也, 第34回日本動物細胞工学会2021年度大会, クマリン類の抗アレルギー効果に関する研究, Oral presentation, 27 Jul. 2021, 27 Jul. 2021, 28 Jul. 2021
  • 竹島大貴; 菊﨑泰枝; 伊藤秀之; 小森博文; 植野洋志; 新田陽子, 日本ビタミン学会第73回大会, エラジタンニンによるヒスチジンでカルボキシラーゼ活性阻害機構の検討, Oral presentation, 19 Jun. 2021, 19 Jun. 2021, 20 Jun. 2021, rm:research_project_id
  • Rika Ohara; Yurika Kitamura; Takuya Sugahara; Hiroyuki Onda; Nanami Yoshino; Hiroe Kikuzaki, ICoFF2019, Constituents of coriander leaves involved in antioxidant and anti-allergic activities, Poster presentation, 04 Dec. 2019, 01 Dec. 2019, 05 Dec. 2019
  • Yoko Nitta; Hideyuki Ito; Hirohumi Komori; Hiroshi Ueno; Hiroe Kikuzaki, ICPH2019, The inhibitory activity of ellagitannins on recombinant human histidine decarboxylase, Poster presentation, 29 Nov. 2019, 28 Nov. 2019, 01 Dec. 2019
  • 上山蓮実; 菊崎泰枝; 北瓜亮太; 餅田尚子; 松村羊子; 栢野新市, 第73回日本栄養・食糧学会, 葛デンプン晒し液より単離した化合物の化学構造解析およびエストロゲン様活性の評価, Oral presentation, 18 May 2019
  • KIKUZAKI Hiroe, 第33回日本香辛料研究会, サバ筋肉中におけるヒスタミン生成を抑制するハーブ・スパイスの探索, Oral presentation, Nov. 2018, 一般社団法人日本香辛料研究会, 味の素株式会社クライアント・イノベーション・センター、神奈川県, False
  • KIKUZAKI Hiroe, 第6回奈良まほろば産学官連携懇話会, 食用植物に含まれるヒスタミン産生抑制成分の探索, Sep. 2018, 奈良まほろば産学官連携懇話会, 奈良女子大学、奈良県, False
  • KIKUZAKI Hiroe, 日本調理科学会平成30年度大会, 加熱調理がカロテノイド及びポリフェノール系成分由来の抗酸化力に及ぼす影響 人参の煮物の場合, Aug. 2018, 一般社団法人日本調理科学会, 武庫川女子大学 兵庫県, False
  • KIKUZAKI Hiroe, 日本農芸化学会2017年度大会, Elucidation of constituents in coriander (Coriandrum sativum L.) leaves and stem based on antioxidant activities, Mar. 2018, 日本農芸化学会, 名城大学、名古屋, False
  • KIKUZAKI Hiroe, 第56回日本栄養・食糧学会近畿支部大会, さとうきび抽出物に含まれる脂質酸化抑制成分の探索と定量分析による主要成分の解明, Nov. 2017, 日本栄養・食糧学会近畿支部, 龍谷大学瀬田キャンパス, False
  • KIKUZAKI Hiroe, 第32回日本香辛料研究会, コリアンダー(Coriandrum sativum L.)の抗酸化性, Sep. 2017, 日本香辛料研究会, 兵庫大学, False
  • KIKUZAKI Hiroe, 第71回日本栄養・食糧学会大会, ローズレッドペタルに含まれるフラボノイド配糖体のヒスチジン脱炭酸酵素阻害活性と阻害様式, May 2017, 日本栄養・食糧学会, 琉球大学, False
  • KIKUZAKI Hiroe, Japanese Society for Food Factors JSoFF2016, Inhibitory activity of flavonol glycosides in the petal of Rosa gallica on histidine decarboxylase., Nov. 2016, 日本フードファクター学会, 富山国際会議場 富山市, False
  • KIKUZAKI Hiroe, 第12回日本給食経営管理学会学術総会, アメリカのFood Serviceテキストと日本の給食経営管理論教科書との比較, Nov. 2016, 日本給食経営管理学会, 大手前大学 さくら夙川キャンパス 兵庫県西宮市, False
  • KIKUZAKI Hiroe, 第60回香料・テルペンおよび精油化学に関する討論会, ショウガ科香辛料に含まれる機能物質の探索, Oct. 2016, 香料・テルペンおよび精油化学に関する討論会, 東京農業大学オホーツクキャンパス 網走市, False
  • KIKUZAKI Hiroe, 日本油化学会第55回年会, Antioxidative constituents in the sugar cane extract., Sep. 2016, 日本油化学会, 奈良女子大学 奈良市, False
  • KIKUZAKI Hiroe, 日本調理科学会平成28年度大会, The antioxidant capacity of polyphenol-rich foods and dishes. -Influence of vitamin C on the antioxidant capacity-, Aug. 2016, 日本調理科学会, 名古屋学芸大学 名古屋市, False
  • KIKUZAKI Hiroe, 第70回日本栄養・食糧学会大会, メチル化カテキンによる分泌型スフィンゴミエリナーゼ阻害作用, May 2016, 日本栄養・食糧学会, 武庫川女子大学, False
  • KIKUZAKI Hiroe, 第9回日本ポリフェノール学会学術大会, 抗酸化物質による分泌型スフィンゴミエリナーゼ阻害作用, Aug. 2015, 日本ポリフェノール学会, 奈良女子大学、奈良市, False
  • KIKUZAKI Hiroe; Keiko Kobayashi; Yuki Ishizaki; Moe Miyamoto; Hiroe Kikuzaki, ACN2015 Asian Congress of Nutrition, Inhibition of secretory sphingomyelinase by epicatechin 3-O-gallate, May 2015, Japan Society of Nutrition and Food Science, Yokohama, Japan, True
  • KIKUZAKI Hiroe, 第87回日本生化学会大会, Epicatechin gallate inhibits secretory shingomyelinase, Oct. 2014, 京都国際会館、京都, False
  • KIKUZAKI Hiroe, 日本家政学会関西支部第36回(通算92回)研究発表会, カテキン類による分泌型スフィンゴミエリナーゼの阻害作用に関する研究, Oct. 2014, 日本家政学会関西支部, 京都聖母女学院短期大学、京都, False
  • KIKUZAKI Hiroe; Hiroe Kikuzaki; Kozue Akimoto; Yuka Watanabe; Keiko Kobayashi; Hiroshi Ueno; Yoko Nitta, XXVIIth International Conference of Polyphenols & 8th Tannin Conference, Inhibitory effects of polyphenols in Rosa gallica on recombinant human histidine decarboxylase, Sep. 2014, ICP2014, Nagoya University, Nagoya, True
  • KIKUZAKI Hiroe, 日本調理科学会平成26年度大会, Appropriateness of using antioxidant capacity of food with the ORACmethod for diet management, Aug. 2014, 日本調理科学会, 広島市 県立広島大学, False
  • KIKUZAKI Hiroe, 第68回日本栄養・食糧学会大会, Inhibitory effect of (-)-epigallocatechin gallate on secretory sphingomyelinase, Jun. 2014, 札幌市 酪農学園大学, False
  • KIKUZAKI Hiroe, 日本農芸化学会2014年度大会, Rosa gallicaの花弁に含まれるヒスチジン脱炭酸酵素阻害活性成分の探索, Mar. 2014, 神奈川県 明治大学, False
  • KIKUZAKI Hiroe, 第20回スパイス&ハーブ研究成果セミナー, 香辛料の抗酸化機能-オールスパイス-, Nov. 2013, 公益財団法人 山崎香辛料振興財団, 東京都 エスビー食品㈱スパイスセンター, False
  • KIKUZAKI Hiroe, 第86回日本生化学会大会, Change of activity of secretory sphingomyelinase and ceramide levels in apoE-/- mice during aging process, Sep. 2013, 横浜, False
  • KIKUZAKI Hiroe, 日本調理科学会平成25年度大会, ORACを指標とした野菜および野菜料理の抗酸化力の評価, Aug. 2013, 日本調理科学会, 奈良市 奈良女子大学, False
  • KIKUZAKI Hiroe, 日本食品科学工学会第60回記念大会 シンポジウムB1「食品ゲルのテクスチャーとフレーバーリリース」, 食品ゲルのテクスチャーとフレーバーリリース\n寒天ゲルからのショ糖のリリース-寒天濃度、ショ糖濃度、ゲル調製法の影響-, Aug. 2013, 東京都 実践女子大学, False
  • KIKUZAKI Hiroe, 第67回日本栄養・食糧学会大会, apoE欠損型マウスにおける老化に伴う動脈硬化進行とセラミド動態に関する研究, May 2013, 名古屋市 名古屋大学, False
  • KIKUZAKI Hiroe, 日本農芸化学会2013年度大会, 酵素グルコシダーゼや腸管モデル培養細胞を用いた配糖体Leptosinの代謝研究, Mar. 2013, 日本農芸化学会, 東北大学, False
  • KIKUZAKI Hiroe, 第17回日本フードファクター学会学術集会, マヌカ蜂蜜から発見された新規配糖体の単離と酵素を用いた構造解析, Nov. 2012, 日本フードファクター学会, 静岡, False
  • KIKUZAKI Hiroe; Shin-ichi Kayano; Yoko Matsumura; Yoko Kitagawa; Mayumi Kobayashi; Asuka Nagayama; Nami Kawabata; Hiroe Kikuzaki; Yoshimi Kitada, 2012 EFFoST Annual Meeting, Isoflavone C-glycosides isolated from the root of Kudzu (Pueraria lobata) and their estrogenic activities, Nov. 2012, European Federation of Food Science and Technology, Montpellier, France, True
  • KIKUZAKI Hiroe, 第8回日本給食経営管理学会学術総会, 給食経営管理実習を利用したエネルギー教育の試み~定格消費電力と作業工程表に基づいた消費電力量の予測と実測~, Nov. 2012, 日本給食経営管理学会, 名古屋女子大学汐路学舎, False
  • KIKUZAKI Hiroe, 日本調理科学会平成24年度大会, ショ糖添加寒天ゲルからのショ糖のリリースと甘味強度, Aug. 2012, 日本調理科学会, 秋田大学, False
  • KIKUZAKI Hiroe, 日本調理科学会平成24年度大会, ショウガ科植物Kaempferia parviflora根茎に含まれる熱に安定な色素について, Aug. 2012, 日本調理科学会, 秋田大学, False
  • KIKUZAKI Hiroe; Z. Wang; K. Yang; H. Kikuzaki; H. Moritaka; K. Nishinari, 2nd International Conference of Food Oral Processing, Physics, Physiology and Psychology of Eating, Sucrose release from agar gels as studied by uniaxial compression and sensory evaluation, Jul. 2012, the Institut National de la Recherche Agronomique and the Royal Society of Chemistry, Beaune, France, True
  • KIKUZAKI Hiroe, 日本家政学会第64回大会, 食品ゲルのテクスチャーとフレーバーリリース 1.ショ糖添加寒天ゲルからのショ糖のリリース, May 2012, 日本家政学会, 大阪市立大学, False
  • KIKUZAKI Hiroe, 日本家政学会第64回大会, 食品ゲルのテクスチャーとフレーバーリリース 2.ショ糖添加寒天ゲルからのショ糖のリリース, May 2012, 日本家政学会, 大阪市立大学, False
  • KIKUZAKI Hiroe, 第66回日本栄養・食糧学会大会, Kaempferia parvifloraから得られたメトキシフラボンの抗アレルギー作用, May 2012, 仙台市 東北大学, False
  • KIKUZAKI Hiroe, 日本農芸化学会2012年度大会, マヌカ蜂蜜に由来する新規配糖体Leptosinのケミカルマーカーとしての有用性について, Mar. 2012, 日本農芸化学会, 京都女子大学, False
  • KIKUZAKI Hiroe, 地域イノベーション戦略支援プログラム 和歌山県紀北紀中エリア\n平成23年度第2階研究交流会, 食用植物に含まれる機能性成分の化学構造と活性, Dec. 2011, 公益財団法人わかやま産業振興財団、和歌山県, 和歌山市, False
  • KIKUZAKI Hiroe, 第7回日本給食経営管理学会学術総会, 給食経営管理実習中のガス・電力消費量の「見える化」および測定によるエネルギー教育の試み, Nov. 2011, 日本給食経営管理学会, 大妻女子大学千代田キャンパス, False
  • KIKUZAKI Hiroe; Yoji Kato; Natsuki Umeda; Asuna Maeda; Noritoshi Kitamoto; Hiroe Kikuzaki, 2011 International Conference on Food Factors, Novel Chemical Marker of Nanuka Honey, Nov. 2011, 2011 International Conference on Food Factors, Taipei, Taiwan, True
  • KIKUZAKI Hiroe, 日本調理科学会平成23年度大会, さとうきび抽出物による鮭の退色抑制効果, Aug. 2011, 日本調理科学会, 高崎健康福祉大学, False
  • KIKUZAKI Hiroe, 日本調理科学会平成23年度大会, 食品ゲルのレオロジー特性とフレーバーリリース, Aug. 2011, 日本調理科学会, 高崎健康福祉大学, False
  • KIKUZAKI Hiroe, 第65回日本栄養食糧学会大会, 葛デンプン晒し液より単離したイソフラボンのエストロゲン様活性及び抗変異原性の評価, May 2011, 日本栄養・食糧学会, False
  • KIKUZAKI Hiroe, 第25回日本香辛料研究会, メドウスウィート(Filipendula ulmaria)成分によるヒスチジン脱炭酸酵素阻害機構の解明, Nov. 2010
  • KIKUZAKI Hiroe, 第15回日本フードファクター学会学術集会, メドウスウィート(Filipendula ulmaria)のヒスチジン脱炭酸酵素阻害成分の探索, Oct. 2010, 仙台, False
  • KIKUZAKI Hiroe, 日本栄養・食糧学会第49回近畿支部大会, タイ原産ナス(Solanum sanitwongsei)の成分, Oct. 2010, False
  • KIKUZAKI Hiroe, 第6回日本給食経営管理学会学術総会, 給食経営管理実習中のガス消費量「見える化」によるエネルギー教育の試み, Oct. 2010, False
  • KIKUZAKI Hiroe, 日本調理科学会平成22年度大会, 大阪特産野菜「葉ゴボウ」に含まれる抗酸化成分の定量と抗酸化成分に対する加熱調理の影響, Aug. 2010, False
  • KIKUZAKI Hiroe, 第64回日本栄養・食糧学会大会, ショウガ科植物Kaempferia parvifloraに含まれるフラボノイドの抗変異原性, May 2010, False
  • KIKUZAKI Hiroe, 第64回日本栄養・食糧学会大会, 葛デンプン晒し液より単離したイソフラボンC-配糖体の化学構造およびエストロゲン様活性, May 2010, False
  • KIKUZAKI Hiroe, 第64回日本栄養・食糧学会大会, Kaempferia parvifloraの抗アレルギー成分, May 2010, 徳島, False
  • KIKUZAKI Hiroe, 日本農芸化学会2010年度大会, ゲットウ (Alpinia zerumbet (Pers.)Burtt and Smith) 種子に含まれる膵リパーゼ阻害成分の解明, Mar. 2010, False
  • KIKUZAKI Hiroe, 第53回香料・テルペンおよび精油化学に関する討論会, マンゴージンジャー(Curcuma amada)に含まれるジテルペノイドの構造, Nov. 2009, False
  • KIKUZAKI Hiroe, 第48回日本栄養・食糧学会近畿支部大会, オンジ(Polygala tenuifolia)からの1,5-アンヒドログルシトールの精製と単離, Nov. 2009, False
  • KIKUZAKI Hiroe, 第14回日本フードファクター学会学術集会, ローズマリーに含まれるポリフェノールの抗酸化能, Nov. 2009, False
  • KIKUZAKI Hiroe, 第24回日本香辛料研究会, メドウスウィート(Filipendula ulmaria (L.) Maxim.)に含まれるヒスチジン脱炭酸酵素阻害成分, Nov. 2009, False
  • KIKUZAKI Hiroe; Hiroe Kikuzaki; Nobuji Nakatani, Italy-Japan Symposium on "Foods and Health: past, present and future", Multiple Functions in Ginger Family, Oct. 2009, Intituo Italiano di Culture, Tokyo, Japan, True
  • KIKUZAKI Hiroe, 第5回日本給食経営管理学会学術総会, アレルギー対応クッキーの品質特性-生地を真空包装し冷凍保存した場合-, Oct. 2009, False
  • KIKUZAKI Hiroe, 第5回日本給食経営管理学会学術総会, 給食経営管理実習時のガス消費量の「見える化」による省エネ意識の変化, Oct. 2009, False
  • KIKUZAKI Hiroe, 第56回日本栄養改善学会学術総会, 皮茸(コウタケ)の抗酸化性および抗変異原性の評価, Sep. 2009, False
  • KIKUZAKI Hiroe, 日本調理科学会平成21年度大会, 低アレルゲン大豆「なごみまる」のイソフラボンおよび脂肪酸分析と「なごみまる」を原料とした味噌の官能評価, Aug. 2009, False
  • KIKUZAKI Hiroe, 第63回日本栄養・食糧学会大会, ナス果肉から単離した成分の抗酸化性の定量的評価, May 2009, False
  • KIKUZAKI Hiroe, 第63回日本栄養・食糧学会大会, 葛デンプン晒し液より単離したイソフラボンのエストロゲン様活性の評価, May 2009, False
  • KIKUZAKI Hiroe, 第23回日本香辛料研究会, ショウガ科植物Kaempferia parvifloraに含まれる色素の構造と熱安定性, Nov. 2008, False
  • KIKUZAKI Hiroe, 第13回日本フードファクター学会, ヒスタミン合成を阻害するオールスパイス成分の探索, Nov. 2008, False
  • KIKUZAKI Hiroe, 第4回日本給食経営管理学会学術総会, スチームコンベクションオーブン加熱における庫内水蒸気濃度が食品の昇温に与える影響, Nov. 2008, False
  • KIKUZAKI Hiroe; Hiroe Kikuzaki; Chiyomi Suzuki; Yuzuri Iwamoto; Nobuji Nakatani, Italian-Japanese Joint Symposium-Natural products and functional foods, Radical scavenging and hyaluronidase inhibitory activities of constituents in Japanese vegetables, Arctiun lappa and Petasites japonicus, Jun. 2008, Salerono, Italy, True
  • KIKUZAKI Hiroe; Nobuji Nakatani; Hiroe Kikuzaki, Joint symposium-Natural products and functional foods, Search for antioxidants in spices and herbs, Jun. 2008, Salerno, Italy, True
  • KIKUZAKI Hiroe, 第62回日本栄養・食糧学会大会, 葛デンプン晒し液から単離したイソフラボンの構造解析および抗変異原性の評価, May 2008, False
  • KIKUZAKI Hiroe, 第62回日本栄養・食糧学会大会, 葉ゴボウ(Arctium lappa)のルチンとクロロゲン酸含量, May 2008, False
  • KIKUZAKI Hiroe, 第62回日本栄養・食糧学会大会, パプアナツメグ、パプアメース(Myristica argentea)に含まれるリグナンのラジカル捕捉活性評価, May 2008, False
  • KIKUZAKI Hiroe, 日本薬学会第128年会, 黒ショウガ(Kaempferia parviflora)根茎に含まれる新規配糖体の構造, Mar. 2008, False
  • KIKUZAKI Hiroe, 日本農芸化学会2008年度大会, ショウガ科植物に含まれる一酸化窒素産生抑制成分, Mar. 2008, False
  • KIKUZAKI Hiroe, 第3回日本給食経営管理学会学術総会, スチームコンベクションオーブンを利用した加熱調理の特性と給食経営管理における有用性の検討, Nov. 2007, False
  • KIKUZAKI Hiroe, 第3回日本給食経営管理学会学術総会, スチームコンベクションオーブンの使用形態とエネルギー消費, Nov. 2007, False
  • KIKUZAKI Hiroe; Hisamoto, M; Okuda, T; Nishihara, N; Kikuzaki, H; Yokotsuka, K, International Conference of Food Factors for Health Promotion-ICoFF2007, Antimicrobial activity of saponins from leaves of Bacopa monnieri against yeast, Nov. 2007, True
  • KIKUZAKI Hiroe; Hiroe Kikuzaki; Chitose furukawa; Ryoji Takagaki; Yasunaga Yamaguchi; Nobuaki Ohto, International Conference of Food Factors for Health Promotion-ICoFF2007, Constituents Enhancing eNOS Protein Expression in the Fruits of Piper longum, Nov. 2007, True
  • KIKUZAKI Hiroe; Amane Takahashi; Norio Yamamoto; Toshiaki Azuma; Kayo Akiyama; Hiroe Kikuzaki; Nobuji Nakatani; Hajime Ohigashi, International Conference on Food Factors for Health Promotion-ICoFF2007, Search for Zingiberaceae Phytochemicals that Suppress NO Generation from Stimulated Macrophages, Nov. 2007, True
  • KIKUZAKI Hiroe; Kayo Akiyama; Nobuji Nakatani; Hiroe Kikuzaki, ICPH2007-3rd International Conference of Polyphenols and Health, Radical Scavenging Capacity of Tannins and Flavonoids Isolated from Salam Leaf (Syzygium polyanthum), Nov. 2007, True
  • KIKUZAKI Hiroe, 日本家政学会関西支部第29回研究発表会, 大阪産野菜「葉ごぼう」のラジカル捕捉活性について, Oct. 2007, False
  • KIKUZAKI Hiroe; Kametani, S; Kojima-Yuasa, A; Kikuzaki, H; Honzawa, M; Matsui-Yuasa, I, 10th Asian Congress of Nutrition, Synergistic Growth Inhibitory Effect ofConstituents of Cape Aloe Extract in Ehrlich Ascites Tumor Cells, Aug. 2007, True
  • KIKUZAKI Hiroe, 第61回日本栄養・食糧学会大会, 黒ショウガ(Kaempferia parviflora)に含まれるフラボノイドのNO産生抑制活性, May 2007, False
  • KIKUZAKI Hiroe, 第61回日本栄養・食糧学会大会, フキ葉身の栄養成分分析, May 2007, False
  • 小原理加; 恩田浩幸; 菊﨑泰枝, 日本調理科学会2022年度大会, コリアンダーの料理に伴う抗酸化活性の変化, 03 Sep. 2022, 02 Sep. 2022, 03 Sep. 2022
  • 金光穂乃花; 池田剛; 石田萌子; 西甲介; 菊崎泰枝; 小原理加; 恩田浩幸; 菅原卓也, 第76回日本栄養・食糧学会大会, コリアンダー葉水溶性抽出物の脱顆粒抑制効果に関する研究, 12 Jun. 2022, 10 Jun. 2022, 12 Jun. 2022
  • 小原理加; 石田萌子; 恩田浩幸; 吉野七海; 菅原卓也; 杉江優奈; 菊崎泰枝, 第76回日本栄養・食糧学会大会, コリアンダー(Coriandrum sativum L.)の抗アレルギー活性と活性寄与成分の解明, 11 Jun. 2022, 10 Jun. 2022, 12 Jun. 2022

Works

  • さとうきび由来成分による食品中の油脂酸化抑制に関する研究, Jun. 2014, Mar. 2015
  • さとうきび由来成分による食品中の油脂酸化抑制に関する研究, Jun. 2013, Mar. 2014
  • ごぼうの有効成分の単離、同定に関する研究, Jun. 2012, Mar. 2013
  • さとうきび由来成分による食品への抗酸化作用に関する研究, Jun. 2012, Mar. 2013
  • さとうきび抽出物の成分解明及び食品に対する抗酸化作用に関する研究, Jun. 2011, Mar. 2012
  • さとうきび抽出物の抗酸化性について, Jun. 2009, Mar. 2010
  • アレルギー除去食品の開発及び幼稚園献立の監修, Aug. 2006, Jul. 2007
  • サクラダソウの成分分析, Apr. 2006, Mar. 2007
  • ヒハツの機能性成分の解明, 2005, 2007
  • マンゴージンジャーの機能性成分の解明, 2004, 2007
  • 化粧品原料ローズマリーエキスの成分分析, Jan. 2006, Jun. 2006
  • カワラヨモギの機能成分の解析, Jul. 2004, Jun. 2005
  • 黒ショウガ成分の構造解明, Jul. 2004, Jun. 2005
  • 竹に含まれる機能成分の解明, Jun. 2004, May 2005
  • プルーン(Prunus domestica L.)に含まれる生理活性物質の精査研究, Nov. 2003, Oct. 2004

Research Projects

  • 01 Dec. 2016, 31 Mar. 2023, Principal investigator
  • 01 Apr. 2020, 31 Mar. 2023, Principal investigator
  • 01 Apr. 2019, 31 Mar. 2023, Principal investigator
  • 10 Feb. 2017, 31 Mar. 2023, Principal investigator
  • Apr. 2020, Mar. 2023, 20K11638, Coinvestigator
  • Apr. 2019, Mar. 2022, 19K11790, Principal investigator
  • 2010, 2012, 食品ゲルの咀嚼過程における呈味成分のリリースに関する研究, 科学研究費補助金, 0, 0, 0, 嗜好性の優れたゲル食品開発を目標とする研究の一部を分担している。具体的には寒天をはじめとする各種食品ゲルの咀嚼過程における各種呈味成分(甘味、塩味、うま味、酸味、苦味、辛味、渋味)の放出を経時的に定量し、放出パターンを調べる。, Competitive research funding
  • 2007, 給食経営管理に関する研究, 0, 0, 0, スチームコンベクションオーブン、真空調理を利用した給食システムに関する研究、給食施設におけるエネルギー消費に関する研究、食事から摂取される抗酸化成分量(ORACを指標として)に関する研究, Competitive research funding
  • 2006, 食用植物に含まれる抗酸化成分の解明と抗酸化特性, 0, 0, 0, 抗酸化性を有する食用植物に含まれる抗酸化成分を各種抗酸化性測定法、ラジカル捕捉活性測定法を用いて、その効力を比較検討する。, Competitive research funding
  • Grant-in-Aid for Scientific Research (B), Nov. 2011, Mar. 2014, 23300270, Assessment of antioxidant capacities of vegetables using the standard antioxidant capacity index of foods and their application to diet management, KIKUZAKI HIROE; KOBAYASHI Keiko, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Nara Women's University, 2470000, 1900000, 570000, The work deals with the matter whether ORAC (Oxygen Radical Absorbance Capacity) which has been established as the standard index of antioxidant capacity of polyphenols is practicable as a tool of nutritional epidemiological study and for diet management to contribute to human health or not. The ORAC, DPPH radical scavenging capacity and total phenolic contents of thirty foodstuffs including mainly vegetables and five cooked dishes made from them as ingredients were measured. The antioxidant capacity and total phenolic content of a portion of each dish were calculated based on the antioxidant capacities and total phenolic content of each 100-g fresh foodstuff, and then the calculated values were compared with the measured values. The results suggested that ORAC could be a more suitable tool for diet management as the antioxidant index than DPPH radical scavenging capacity., url
  • Grant-in-Aid for Scientific Research (C), Apr. 2015, Mar. 2018, 15K00783, Constituents in some edible plants inhibiting the activity of histamine producing enzyme, histidine decarboxylase, and their application to prevention of food poisoning caused by histamine, KIKUZAKI HIROE, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Nara Women's University, 4810000, 3700000, 1110000, To prevent food poisoning caused by histamine which has been on the increase, it is very important that histamine accumulation should be arrested at the stage of distribution and storage because histamine is not detoxified by cooking for its stability against heat. In this study, we searched for constituents in some edible plants that inhibit the activity of histamine producing enzyme, histidine decarboxylase, derived from adherent germ to food and production of histamine in fish. Six ellagitannins in meadowsweet, petal of rose red or cloves were identified as strong inhibitors of the enzyme. It is likely that a short-time immersion of fish in the hot-water extracts of these plants protects accumulation of histamine based on ellagitannin content of the extracts., url
  • Grant-in-Aid for Scientific Research (B), 2007, 2009, 19300247, Structures of functional constituents in vegetables cultivated in Osaka, Japan and their properties on cooking, KIKUZAKI Hiroe; NAKATANI Nobuji; IWAMOTO Yuzuri, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Osaka City University, 11700000, 9000000, 2700000, In the course of our elucidation of structures of functional constituents in vegetables cultivated in Osaka and their properties on cooking, 14 compounds including hyaluronidase inhibitory and antioxidative fukinolic acid and 3,4,5-tri-O-caffeoylquinic acid from leaves of Petasites japonicus and nine compounds including antioxidative chlorogenic acid and rutin from leaves of Arctium lappa were isolated and their structures were determined. The seasonal variation of antioxidants in A.lappa indicated that the content of antioxidants reached the maximum at the best season. Furthermore, both P.japonicus and A.lappa were rich in vitamins. Boiling of leaves of A.lappa resulted in 50% elution of antioxidants into boiling water., url
  • Grant-in-Aid for Scientific Research (C), 2005, 2006, 17500547, Systematic evaluation of radical scavenging active compounds in some edible plants using some different methods, KIKUZAKI Hiroe; NAKATANI Nobuji, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Osaka City University, 3100000, 3100000, This study was aimed to evaluate in vitro radical scavenging activity of some edible plant constituents using some different methods and elucidate chemical structure and activity relationship of these antioxidants. Firstly, ten lipophilic compounds were isolated from Papuan nutmeg and mace (Myristica argentea) and their structures were elucidated on the basis of spectroscopic evidences of NMR and MS, resulting in determination of six dimethylbutane lignans and four tetrahydrofuran lignans. Radical scavenging activities of these isolated lignans were measured by three different methods, evaluation of scavenging effects on DPPH radical and O2- radical, and evaluation of ORAC (Oxygen Radical Absorbance Capacity). The activities measured by both DPPH radical scavenging and ORAC methods seemed to be dependent on the number of hydroxyl groups on the benzene ring. However, lignans having benzyl methylene tended to show a higher scavenging ability against DPPH radical than lignans having oxygenated benzylic carbon, while tended to show a lower ORAC. On the other hand, no clear relationship was observed between the chemical structure of lignans and their scavenging activity against O2- radical. Secondarily, five hydrophilic compounds were isolated from salam leaves (Syzygium polyanthum) and they were identified as two myricetin glycosides and three ellagitannins. The radical scavenging activities of these five compounds together with some tannins and flavonoids which had been previously isolated from the ethyl acetate soluble fraction were measured using three different method mentioned above. The DPPH radical scavenging activity was dependent on the number of hydroxyl groups on the benzene ring. On the contrary, comparing the O2- radical scavenging activity in five ellagitannins having the same number of ortho-trihydroxyphenyl (o-THP) groups indicated that the more the number of hexahydroxydiphenoyl (HHDP) groups were in the molecule, the more the activity was. On the other hand, ORAC of tannins having more than three o-THP groups in the molecule was not influenced by the number of galloyl and HHDP groups. Consequently, the radical scavenging abilities obtained by three different methods were not necessarily the same, therefore, more than one method are required to evaluate antioxidants.
  • Grant-in-Aid for Scientific Research (B), 2004, 2005, 16300238, Isolation and Structure Elucidation of Antioxidants of Traditional Vegetables and Fruits of Yaeyama Islands in Okinawa., NAKATANI Nobuji; KIKUZAKI Hiroe, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), The University of the Air, 8700000, 8700000, It is our purpose to find and clarify of chemical constituents, in particularly natural antioxidants, which might have supported health and longevity of people in Okinawa. Antioxidants play and important role not only in food preservation but in couteracting the risk of most cardiovascular diseases, cancer and aging. Based on our screening for antioxidant activity in Okinawan traditional edible plants, we have focused our attention on two species, such as Japanese long pepper (Piper retrofractum) and Noni (Morinda citrifolia). Both species showed strong andtioxidant activity evaluated by several methods, such as the OSI method and the DPPH radical scavenging test. From the ethyl acetate soluble fraction of P retrofractum extract, several antioxidative polyphenols including flavonoid glycosides were isolated and determined. From the leaves of M citrifolia, 8 compounds were determined including quercetin glycosides, iridoids and ferulic acid glycosides.
  • Grant-in-Aid for Scientific Research (C), 2003, 2004, 15500547, Antioxidant properties and factors of allspice constituents, KIKUZAKI Hiroe, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Osaka City University, 2900000, 2900000, This study was aimed to determine the structures of allspice constituents and elucidate their antioxidant properties as well as the factors of antioxidant activity. Dried allspice was successively extracted with n-hexane, dichloromethane and 70% aqueous acetone at room temperature. After removing acetone from the 70% aqueous acetone extract, the resulting aqueous residue was separated into the dichloromethane-soluble, ethyl acetate-soluble and water-soluble fractions. The ethyl acetate-soluble fraction which showed a strong DPPH radical scavenging activity and a high inhibitory effect on the autoxidation of methyl linoleate in bulk system was purified using repeated chromatographic techniques to give twenty compounds including two phenylpropanoids, eleven flavonoids and seven gallic acid related compounds. Their structures were elucidated on the basis of some spectral data. Among them, five are new and fifteen are known. However ten known compounds were first isolated from allspice. The antioxidant properties of these isolated compounds together with seventeen compounds which have been already isolated in our laboratory were evaluated using several different methods. The DPPH radical scavenging activity was dependent on the number of ortho-diphenols. The O_2^- radical scavenging activity of compounds with 1,2,3-trihydroxyphenyl groups is stronger than that of compounds with 1,2-dihydroxyphenyl groups. Ortho-diphenol structure was contributed to the inhibitory effects on autoxidation of methyl linoleate in bulk system. When measured at 90℃, the high polar compounds such as tannin and flavonoid glycosides showed slight activity, which suggested that affinity to substrate oil might have some influence on their activity in bulk system at 90℃. Such phenomenon was not observed when measured at 40℃. Tannin showed very strong activity toward liposome oxidation induced by AAPH. In the case of flavonoid, quercetin related compounds showed stronger activity than myricetin related compounds. In addition, the inhibitory effects of six allspice constituents on the autoxidation of dressing at 20℃ were measured. The activity order was consistent with the scavenging activity order against DPPH and in this case the polarity of tested compounds did not influence the activity. In conclusion, compounds with ortho-diphenol structure contributed to the antioxidant effect of allspice, and their antioxidant properties might be governed by their radical scavenging abilities and polarities.
  • Grant-in-Aid for Scientific Research (A), 2001, 2003, 13308007, Search for Antiageing Factors in the Okinawa Diet, NAKATANI Nobuji; MORIMITSU Yasujiro; NISHIKAWA Yoshikazu; KIKUZAKI Hiroe, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Osaka City University, 29250000, 22500000, 6750000, Okinawa is the most longevity prefecture in Japan. Our attention has been paid on the diet in Okinawa to find out some antiageing factors such as biologically protecting ability and diseases preventing components from edible plants traditionally used. This report is compiled from four subjects. First, antioxidant activity was screened for tropical plants leaves traditionally used as food and for food preservation in Okinawa. Remarkably active antioxidants, some flavonoids and chlorogenic acid analogues as major antioxidants, were isolated and determined from the leaves of Peucedanum joponicum, BOTANBOUFU in Japanese. Second, several compounds were structurally identified in Asplenium antiquum, OOTANIWATARI, showing strong antimicrobial activity against bacteria. Third, the extracts of some vegetables and fruits in Okinawa were examined for preventing thrombosis. Polymethoxyflavonoids, such as nobiletin and tangeretin, in Citrus depressa Hayata showed significant antiplatelet activity. Last, a system to evaluate the immunoenhancing effects was established using natural killer (NK) cells. This method must be widely useful to examine for prevention factors against cancer and other diseases. In conclusion, valuable and promising results were obtained for antiageing and improvement for human health for elderly people by this research grant.
  • Grant-in-Aid for Scientific Research (C), 2001, 2002, 13680155, Behavior of antioxidants under some cooking conditions, KIKUZAKI Hiroe, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Osaka City University, 3700000, 3700000, This study was aimed to elucidate what behavior antioxidants from food showed under cooking condition. Two spices, curry leaves (the leaves of Murraya koenigii) and ginger were investigated. When dried curry leaves were added to the substrate oil including 10% methyl linoleate at 110℃, addition of only 0.1% of curry leaves was enough to delay oxidation of the substrate oil, which suggested that curry leaves could have antioxidant effect under heat-cooking condition. Curry leaves were extracted with dichloromethane and the extract was purified using repeated chromatographic techniques to isolate six compounds, which were determined to be carbazoles based on the spectroscopic evidences. Among them, three compounds each of which had a hydroxyl group on the carbazole skeleton showed a strong antioxidant activity. Especially mahanine, a major carbazole, had the strongest ability, which promoted us to elucidate its behavior under heat oxidation. During oxidation, mahanine gradually decreased. Immediately after mahanine was exhausted, oxidation of the substrate oil was progressed radically. Furthermore, there was no marhanine derivative detected which remarkably increased during oxidation. These facts suggested that mahanine was stable under heating condition and directly delayed oxidation of the oil. In the case of ginger, the behavior of [6]-gingerol, a major antioxidant of ginger, under some cooking conditions was investigated. The squeezed juice of ginger passing through 40 mesh possessed about two thirds of antioxidant capacity and [6]-gingeol contents compared with ginger before squeezing. When grated ginger was kept at room temperature for 24 hr, the antioxidant activity decreased significantly whereas [6]-gingerol content did not significantly decrease, which suggested that other antioxidants might decrease. When minced ginger was flied for 3 min, ginger maintained the antioxidant activity and [6]-gingerol content, which indicated that [6]-gingerol could be stable enough to exhibit its antioxidant effect after short dry cooking.
  • Grant-in-Aid for Scientific Research (C), 1998, 1999, 10660133, Structure-activity relationship of antioxidative components isolated from ginger (Zingiber officinal), KIKUZAKI Hiroe, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Osaka City University, 3100000, 3100000, Eleven gingerol related compounds, gingerols, shogaols and dehydrogingerdiones substituted with an alkyl group bearing 8〜12 carbon chain length, were isolated from rhizomes of ginger (Zingiber officinale) using repeated chromatographic techniques. The antioxidant activities of these compounds were evaluated by five measurements ; 1) the DPPH radical scavenging activity, 2) the inhibitory effect on autoxidation of linoleic acid in ehtanol-buffer system, 3) the inhibitory effect on autoxidation of methyl linoleate, 4) the inhibitory effect on oxidation of methyl linoleate under aeration and heating using Oil Stability Index method and 5) the inhibitory effect on oxidation of liposome. The antioxidant activity of these constituents based on the methods 1 and 5 was in the order shogaols 【similar or equal】 gingerols > dehydrogingerdiones. The inhibitory effect on autoxidation of linoleic acid was in the order shogaols > gingerols > dehydrogingerdiones, while the activity of the compounds based on methods 3 and 4 was in the order dehydrogingerdiones > gingerols 【similar or equal】 shogaols. Concerning ginerol related compounds with different lengths of alkyl chain, significant higher activity was correlated with longer alkyl chains based on the method 2. This finding was also observed in the case of alkyl ferulates with different lengths of alkyl chain. In addition, the effects of cinnamic acid related compounds were measured using the methods 1-4. Caffeic acid with 3,4-dihydroxy phenyl group showed the strongest activity based on the methods 1, 3 and 4, while felulic acid with 4-hydroxy-3-methoxyphenyl group showed the strongest activity based on the method 2. These findings suggested that not only radical scavenging ability of antioxidant but also the affinity of the antioxidant with substrate owing to the substituents on the alkyl chain might contribute to the antioxidant activity.
  • Grant-in-Aid for Scientific Research (B), 1996, 1998, 08458008, Elucidation of The Functional Constituents of Plants Traditionally Used for Food Preservation, NAKATANI Nobuji; KIKUZAKI Hiroe; KONISHI Yotaro, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Osaka City University, 7100000, 7100000, This study has aimed to find and use natural products which possess the properties to prevent food deterioration. In this research we have focused on the plant leaves which have been traditionally used to wrap foods. These plants might contain some useful properties for food preservation such as antioxidant and antimicrobial activity. We selected promising eleven plants collected mainly in Okinawa islands based on our survey. The extracts of those leaves were examined for antioxidant activity and radical scavenging ability. Four of them showed remarkable antioxidant activity. From YOMOGI (Artemisia princeps ), 7 polyphenols were isolated as antioxidants including dicaffeoylquinic acids. Kaempherol was isolated from KAKI (Diospyros kaki) leaves, which are used to wrap SUSHI in Yoshino, was revealed to show high antimicrobial activity against Streptocuccus mutans, a tooth-decaying bacteria. Five antimicrobial compounds were found from IRIOMOTEKUMATAKERAN (Alpinia flavellata) and determined their structures including two new distyrylcyclobutenoids. All compounds strongly inhibited the growth of Streptococcus aureus. Our present study suggests that the constituents of the above plants could be useful for food preservation.
  • 共同研究, さとうきび由来成分による食品中の油脂酸化抑制に関する研究, 奈良女子大学
  • Apr. 2019, Mar. 2022, 19K11790, Principal investigator
  • Apr. 2020, Mar. 2023, 20K11638, Coinvestigator

Ⅲ.社会連携活動実績

1.公的団体の委員等(審議会、国家試験委員、他大学評価委員,科研費審査委員等)

  • 日本フードファクター学会, 評議員, Society
  • Society
  • 日本調理科学会, 近畿支部委員, Society
  • Society
  • 日本家政学会, 関西支部委員, Society
  • Society
  • 日本栄養・食糧学会, 近畿支部評議委員, Society
  • Society
  • 香料・テルペンおよび精油化学に関する討論会, 役員, Society
  • Society
  • 日本香辛料研究会, 役員, Society
  • Society
  • 日本家政学会, 代議員, Society
  • Society
  • 日本給食経営管理学会, 評議員, Society
  • Society
  • 日本農芸化学会, 代議員, Society
  • Society
  • 日本調理科学会, 本部代議員, Society
  • Society
  • 日本調理科学会, 近畿支部執行部, Society
  • Society
  • 日本栄養改善学会, 評議員, Society
  • Society
  • AOU研究会, 評議委員, Society
  • Society
  • 日本栄養・食糧学会, 英文誌編集委員, Society
  • Society
  • 日本フードファクター学会, 理事, Society
  • Society
  • 日本調理科学会, 本部理事, Society
  • Society


Copyright © MEDIA FUSION Co.,Ltd. All rights reserved.