Researchers Database

KIKUZAKI Hiroe

    Faculty Division of Human Life and Environmental Sciences Research Group of Food Science and Nutrition Professor
Last Updated :2021/06/02

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Degree

  • Doctor of Human Life Science, Osaka City University

Research Interests

  • Food Chemistry, Natural Organic Chemistry, Cooking Science, Food Service Management, Edible plants, Spices and Herbs, Structure determination, Antioxidants, Enzyme inhibitor, Antiallergic activity, Nutraceuticals 

Research Areas

  • Humanities & social sciences, Home economics, lifestyle science
  • Life sciences, Bioorganic chemistry
  • Life sciences, Food sciences

Research Experience

  • Apr. 2010, Department of Food Science & Nutrition, Nara Women's University
  • Apr. 2003 Mar. - 2010, Graduate School of Human Life Science, Osaka City University・Associate Professor
  • Apr. 1999 Mar. - 2003, A lecturer in Faculty of Human Life Science, Osaka City University
  • Oct. 1996 Mar. - 1999, An assistant in Faculty of Human Life Science, Osaka City University
  • Oct. 1984 Sep. - 1996, A technical employee in Faculty of Human Life Science, Osaka City University

Education

  • Apr. 1984, Sep. - 1984, Osaka City University, Graduate School of Human Life Science, Division of Food and Nutrition
  • Apr. 1978, Mar. - 1982, Osaka City University, Faculty of Human Life Science, Department of Food and Nutrition

Committee Memberships

  • 日本フードファクター学会評議員
  • 日本調理科学会近畿支部委員
  • 日本家政学会関西支部委員
  • 日本栄養・食糧学会近畿支部評議委員
  • 香料・テルペンおよび精油化学に関する討論会役員
  • 日本香辛料研究会役員
  • 日本家政学会代議員
  • 日本給食経営管理学会評議員
  • 日本農芸化学会代議員
  • 日本調理科学会本部代議員
  • 日本調理科学会近畿支部執行部
  • 日本栄養改善学会評議員
  • AOU研究会評議委員
  • 日本栄養・食糧学会英文誌編集委員
  • 日本フードファクター学会理事
  • 日本調理科学会本部理事

Published Papers

  • Effects of cooking methods on free amino acid contents in vegetables

    Ito, H; Kikuzaki, H; Ueno, H

    2019, J. Nutr. Sci. Vitam., 65, 264 - 271

    Scientific journal

  • Free Amino Acid Compositions for Fruits

    KIKUZAKI Hiroe; Hinako Ito; Hiroshi Ueno

    Nov. 2017, Journal of Nutrition and Dietetic Practice, 1 (1), 1-5

  • Novel inhibitors for histidine decarboxylase from plant components

    KIKUZAKI Hiroe; Yoko Nitta; Hiroshi Ueno

    Aug. 2017, International Biology Review, 1 (2), 1-14

  • Inhibition of recombinant human histidine decarboxylase activity in different strawberry cultivars

    Uno, Yuichi; Nitta, Yoko; Ishibashi, Misaki; Noguchi, Yuji; Kikuzaki, Hiroe

    Strawberry fruit contains many constituents, some of which have the potential to inhibit histidine decarboxylase (HDC) activity. HDC converts L-histidine to histamine, which is associated with allergic and other biological reactions in the human body. The HDC inhibition levels were different and the component ratios varied by genotype in strawberry. Among the 11 cultivars collected locally in Japan, 'Tokun' had an approximately ten times higher inhibition ratio than the lowest cultivar. The reproducibility was confirmed using five cultivars under the same conditions in a glass greenhouse, suggesting that genotypic variation is a major factor of HDC inhibition. The potential inhibitors of HDC might be polyphenols because they showed moderate correlations with HDC inhibition rates. Among the polyphenols, the anthocyanin content possessed a moderate negative correlation. Ascorbic acid, which contributes to the overestimation of total polyphenol, did not independently inhibit HDC activity. These findings will support the identification of potential HDC inhibitors in strawberry and indicated that genotypic differences would make useful probes for inhibitor identification., SPRINGER HEIDELBERG, Jun. 2017, ACTA PHYSIOLOGIAE PLANTARUM, 39 (6), web_of_science;doi

    Scientific journal

  • ヒトEGFR過剰発現線虫を用いたビフィズス菌のプロバイオティクス効果

    小村 智美; Shim Jaegal; 岩城 啓子; 菊崎 泰枝; 西川 禎一

    (公社)日本栄養・食糧学会, Apr. 2017, 日本栄養・食糧学会大会講演要旨集, 71回, 282 - 282

  • Construction of a free-form amino acid database for vegetables and mushrooms

    KIKUZAKI Hiroe; Hinako Ito; Hiroshi Ueno

    Apr. 2017, Integrative Food, Nutrition and Metabloism, 4 (4), 1-9, doi

  • Strong Inhibition of Secretory Sphingomyelinase by Catechins, Particularly by (-)-Epicatechin 3-O-Gallate and (-)-3 '-O-Methylepigallocatechin 3-O-Gallate

    Keiko Kobayashi; Yuki Ishizaki; Shosuke Kojo; Hiroe Kikuzaki

    Sphingomyelinases (SMases) are key enzymes involved in many diseases which are caused by oxidative stress, such as atherosclerosis, diabetes mellitus, nonalcoholic fatty liver disease, and Alzheimer's disease. SMases hydrolyze sphingomyelin to generate ceramide, a well-known pro-apoptotic lipid. SMases are classified into five types based on pH optimum, subcellular localization, and cation dependence. Previously, we demonstrated that elevation of secretory sphingomyelinase (sSMase) activity increased the plasma ceramide concentration under oxidative stress induced by diabetes and atherosclerosis in murine models. These results suggest that sSMase inhibitors can prevent the progress of these diseases. The present study demonstrated that sSMase activity was activated by oxidation and inhibited by reduction. Furthermore, we examined whether catechins inhibited the sSMase activity in a physiological plasma concentration. Among catechins, (-)-epicatechin 3-O-gallate (ECg) exhibited strong inhibitory effect on sSMase (IC50=25.7 mu m). This effect was attenuated by methylation at the 3 ''- or 4 ''-position. On the other hand, (-)-epigallocatechin 3-O-gallate (EGCg) and (-)-catechin 3-O-gallate (Cg) exhibited weaker inhibitory activity than ECg, and (-)-epicatechin and (-)-epigallocatechin did not affect sSMase activity. Additionally, one synthetic catechin, (-)-3'-O-methylepigallocatechin 3-O-gallate (EGCg-3'-O-Me), showed the strongest inhibitory effect (IC50=1.7 mu m) on sSMase. This phenomenon was not observed for (-)-4'-O-methylepigallocatechin 3-O-gallate. These results suggest that the reduction potential, the presence of the galloyl residue at the C-3 position, and the steric requirement to interact with sSMase protein are important for effective inhibition of sSMase., CENTER ACADEMIC PUBL JAPAN, Apr. 2016, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 62 (2), 123 - 129, web_of_science

    Scientific journal

  • Inhibition of Morganella morganii Histidine Decarboxylase Activity and Histamine Accumulation in Mackerel Muscle Derived from Filipendula ulumaria Extracts

    Yoko Nitta; Fumiko Yasukata; Noritoshi Kitamoto; Mikiko Ito; Motoyoshi Sakaue; Hiroe Kikuzaki; Hiroshi Ueno

    Filipendula ulmaria, also known as meadowsweet, is an herb; its extract was examined for the prevention of histamine production, primarily that caused by contaminated fish. The efficacy of meadowsweet was assessed using two parameters: inhibition of Morganella morganii histidine decarboxylase (HDC) and inhibition of histamine accumulation in mackerel. Ellagitannins from F. ulmaria (rugosin D, rugosin A methyl ester, tellimagrandin II, and rugosin A) were previously shown to be potent inhibitors of human HDC; and in the present work, these compounds inhibited M. morganii HDC, with half maximal inhibitory concentration values of 1.5, 4.4, 6.1, and 6.8 respectively. Application of the extracts (at 2 wt%) to mackerel meat yielded significantly decreased histamine accumulation compared with treatment with phosphate-buffered saline as a control. Hence, F. ulmaria exhibits inhibitory activity against bacterial HDC and might be effective for preventing food poisoning caused by histamine., INT ASSOC FOOD PROTECTION, Mar. 2016, JOURNAL OF FOOD PROTECTION, 79 (3), 463 - 467, doi;web_of_science

    Scientific journal

  • Anti-allergenic activity of polymethoxyflavones from Kaempferia parviflora

    Shoko Kobayashi; Taro Kato; Toshiaki Azuma; Hiroe Kikuzaki; Keiko Abe

    The inhibitory effects of 13 polymethoxyflavones (PMFs) obtained from Kaempferia paruiflora extracts were evaluated on RBL-2H3 cell degranulation. These flavones suppressed antigeninduced degranulation in RBL-2H3 cells. 5-Hydroxy-3,7,4',-trimethoxyflavone (KP02) and 5,3'-dihydroxy-3,7,4',-trimethoxyflavone (KP10) showed potent inhibitory activities. KP10 significantly suppressed calcium ionophores, such as A23187 and 2,5-ditert-butylhydroquinone, which promoted calcium influx from the outside of cells and from the endoplasmic reticulum, respectively. However, these activities did not fully explain the PMFs' effects. Immunoblot analysis of KPO2 and KP10 showed that the inhibitory effects were attributable to the downregulation of PLC gamma 1 kinase phosphorylation in the signaling pathways involved in degranulation. The phosphorylation levels of Syk were also suppressed by the addition of KP10. KP02 and KP10 treatment to the cells did not affect the high affinity IgE receptor (Fc epsilon RI) levels inside RBL-2H3 cells, but they suppressed the Fc epsilon RI levels in the plasma membrane. Therefore, K. parviflora PMFs have multiple degranulation mechanisms to suppress RBL-2H3 cells and would be beneficial for alleviating type I allergy symptoms. (C) 2014 Elsevier Ltd. All rights reserved., ELSEVIER SCIENCE BV, Mar. 2015, JOURNAL OF FUNCTIONAL FOODS, 13, 100 - 107, doi;web_of_science

    Scientific journal

  • Sucrose release from agar gels: Correlation with sucrose content and rheology

    Kun Yang; Zheng Wang; Tom Brenner; Hiroe Kikuzaki; Yapeng Fang; Katsuyoshi Nishinari

    The effect of sucrose and agar concentrations on sucrose release from agar gels was investigated. It was found that the sucrose release ratio decreased with increasing sucrose concentration and with increasing fracture stress, which is consistent with previous findings. High correlation was found between the fracture stress, sucrose concentration and the sucrose release ratio. The total amount of sucrose released showed a maximum at a certain sucrose concentration, indicating that sucrose addition to enhance sweetness intensity may be ineffective or even counterproductive beyond a certain limit. (C) 2014 Elsevier Ltd. All rights reserved., ELSEVIER SCI LTD, Jan. 2015, FOOD HYDROCOLLOIDS, 43 (1), 132 - 136, doi;web_of_science

    Scientific journal

  • Sucrose release from agar gels: Effects of dissolution order and the network inhomogeneity

    Kun Yang; Zheng Wang; Tom Brenner; Hiroe Kikuzaki; Yapeng Fang; Katsuyoshi Nishinari

    The effect of sucrose addition prior to or following the dissolution of agar on sucrose release from 1 wt% agar gels was studied. The order of addition was found to affect the fracture stress, fracture strain and sucrose release ratio. At sucrose concentrations below 30%, the rheology (fracture stress and strain) and structure of the gels did not depend on the order of addition of sucrose and agar. At sucrose concentrations >40%, however, the network structure of gels where sucrose was dissolved before agar addition was weaker than that of gels where sucrose was added after agar dissolution. The weaker network structure resulted in lower fracture stress and strain values. For gels prepared by adding agar to sucrose solutions, both fracture stress and strain decreased with increasing sucrose concentration in the range 40-55%. The decrease in fracture stress and strain led to an increase in the sucrose release ratio. The sucrose release ratio decreased monotonously with increasing concentration of sucrose when agar was dissolved prior to sucrose addition. The findings are discussed in relation to recent publications on gel inhomogeneity. (C) 2014 Elsevier Ltd. All rights reserved., ELSEVIER SCI LTD, Jan. 2015, FOOD HYDROCOLLOIDS, 43 (1), 100 - 106, doi;web_of_science

    Scientific journal

  • The influence of agar gel texture on sucrose release

    Zheng Wang; Kun Yang; Tom Brenner; Hiroe Kikuzaki; Katsuyoshi Nishinari

    The relationship between sucrose release and texture of food gels was investigated by rheological measurements. Agar gels were selected as model food gel, and sucrose was used as a sweetener. Large deformation uniaxial compression tests were carried out at different compression speeds. The fracture stress increased with increasing agar concentration, while the concentration dependence of the fracture strain was different at different compression speeds. The size-distribution of fragments obtained after repeated compression-cycles was quantified using image analysis. The number and total surface area of fragments decreased with increasing agar concentration. Increasing the compression speed led to an initial increase followed by a monotonous decrease of both the number of fragments and their total surface area. The gel texture affected in a similar fashion both the sucrose release ratio and the total fragment surface area. The sucrose release ratio decreased with increasing agar concentration. (C) 2013 Elsevier Ltd. All rights reserved., ELSEVIER SCI LTD, May 2014, FOOD HYDROCOLLOIDS, 36, 196 - 203, doi;web_of_science

    Scientific journal

  • Glucosyl terpenates from the dried fruits of Prunus domestica L.

    Shin-ichi Kayano; Hiroe Kikuzaki; Sachiko Hashimoto; Kumi Kasamatsu; Takao Ikami; Nobuji Nakatani

    The chemical structures of 9 compounds isolated from the dried fruits of Prunus domestica L. were elucidated on the basis of NMR and MS analyses. Each isolated compound was determined to be scopolin (1), (3-O-cis-p-coumaroyl-beta-D-fructofuranosyl)-(2 -> 1)-alpha-D-glucopyranoside (2), 1S-(4-beta-D-glucopyranosyl- 3-methoxyphenyl)-2R-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol (3), beta-D-glucopyranosyl 9-carboxy-8-hydroxy-2,7-dimethyl-2E, 4E-nonadienate (4), beta-D-glucopyranosyl 7-carboxy2- methyl-2E, 4E-octadienate (5), 8-hydroxy-2,7-dimethyl-2E, 4E-decadienedioic acid 1-beta-D-glucopyranyl ester 10-methyl ester (6), (3-O-trans-p-coumaroyl-beta-D-fructofuranosyl)-(2 -> 1)-alpha-D-glucopyranoside (7), b-D-glucopyranosyl cinnamate (8), and 2,7-dimethyl-2E, 4E-octadienedioic acid (9), respectively. Compounds 2, 3, 4, 7, 8, and 9 were isolated from P. domestica L. for the first time, and compounds 5 and 6 were novel glucosyl terpenates. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved., ELSEVIER SCIENCE BV, May 2014, PHYTOCHEMISTRY LETTERS, 8, 132 - 136, doi;web_of_science

    Scientific journal

  • Increase in Secretory Sphingomyelinase Activity and Specific Ceramides in the Aorta of Apolipoprotein E Knockout Mice during Aging

    Keiko Kobayashi; Eri Nagata; Kazuki Sasaki; Mariko Harada-Shiba; Shosuke Kojo; Hiroe Kikuzaki

    Atherosclerosis is caused by many factors, one of which is oxidative stress. We recently demonstrated that systemic oxidative stress increased secretory sphingomyelinase (sSMase) activity and generated ceramides in the plasma of diabetic rats. In addition, we also showed that the total ceramide level in human plasma correlated with the level of oxidized low-density lipoprotein. To investigate the relationship between ceramide species and atherogenesis during aging, we compared age-related changes in ceramide metabolism in apolipoprotein E knock out mice (apoE(-/-)) and wild type mice (WT). Although the total plasma ceramide level was higher in apoE(-/-) than that in WT at all ages, it decreased with increasing age. sSMase activity increased at 65 weeks (w) of age in both strains of mice. When apoE(-/-) developed atherosclerosis at 15 w of age, C18:0, C22:0, and C24:0 ceramide levels in the apoE(-/-) aorta significantly increased. Furthermore, at 65 w of age C16:0 and C24:1 ceramide levels were significantly higher than those in WT. These results suggested that elevation in levels of specific ceramide species due to sSMase activity contributed to atherogenesis during aging., PHARMACEUTICAL SOC JAPAN, Jul. 2013, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 36 (7), 1192 - 1196, web_of_science

    Scientific journal

  • Inhibitory activity of Filipendula ulmaria constituents on recombinant human histidine decarboxylase

    Yoko Nitta; Hiroe Kikuzaki; Toshiaki Azuma; Yuan Ye; Motoyoshi Sakaue; Yoshiki Higuchi; Hirohumi Komori; Hiroshi Ueno

    Histidine decarboxylase (HDC) catalyses the formation of histamine, a bioactive amine. Agents that control HDC activity are beneficial for treating histamine-mediated symptoms, such as allergies and stomach ulceration. We searched for inhibitors of HDC from the ethyl acetate extract of the petal of Filipendula ulmaria, also called meadowsweet. Rugosin D, rugosin A, rugosin A methyl ester (a novel compound), and tellimagrandin II were the main components these 4 ellagitannins exhibited a non-competitive type of inhibition, with Ki values of approximately 0.35-1 μM. These Ki values are nearly equal to that of histidine methyl ester (Ki = 0.46 μM), an existing substrate analogue inhibitor. Our results show that food products contain potent HDC inhibitors and that these active food constituents might be useful for designing clinically available HDC inhibitors. © 2012 Elsevier Ltd. All rights reserved., 01 Jun. 2013, Food Chemistry, 138 (2-3), 1551 - 1556, doi;pubmed

    Scientific journal

  • Attempt of energy education with estimation and measurement of electric power consumption during institutional food seryvice management practicum

    KIKUZAKI Hiroe

    May 2013, 日本給食経営管理学会誌, 7 (1), 31-42

  • Isoflavone C-glycosides isolated from the root of kudzu (Pueraria lobata) and their estrogenic activities

    Shin-ichi Kayano; Yoko Matsumura; Yoko Kitagawa; Mayumi Kobayashi; Asuka Nagayama; Nami Kawabata; Hiroe Kikuzaki; Yoshimi Kitada

    The chemical structures of six isoflavones (1-6) isolated from the kudzu root (Pueraria lobata) were elucidated on the basis of the NMR and MS analyses to be four isoflavone C-glycosides as 6 ''-O-alpha-D-glucopyranosylpuerarin (1), puerarin (2), 3'-methoxypuerarin (3), 6 ''-O-alpha-D-apiofranosylpuerarin (4), and two aglycons as biochanin A (5) and formononetin (6), respectively. The estrogenic activities of isolated compounds and related isoflavones were evaluated using a yeast two-hybrid assay. Genistein exhibited the highest activity among the evaluated compounds at 10(-6) M followed by daidzein (10(-5) M), baiochanin A (5) (10(-5) M), daidzin (10(-5) M), 3',4',7-trihydroxyisoflavone (10(-3) M), and formononetin (6) (10(-2) M). The isoflavone C-glycosides (1-4) and 4',7-dimethoxyisoflavone showed no activities. In these results, it was found that the addition of 8-C-glucose or 3'-hydroxyl group to daidzein skeleton, or the substitution of hydroxyl functions to methoxyl groups, decreased the estrogenic activity of daidzein, on the other hand, the addition of 7-O-glucose moiety had no influence on the activity. (C) 2012 Elsevier Ltd. All rights reserved., ELSEVIER SCI LTD, Sep. 2012, FOOD CHEMISTRY, 134 (1), 282 - 287, doi;web_of_science

    Scientific journal

  • Identification of a Novel Glycoside, Leptosin, as a Chemical Marker of Manuka Honey

    Yoji Kato; Natsuki Umeda; Asuna Maeda; Daiki Matsumoto; Noritoshi Kitamoto; Hiroe Kikuzaki

    As a preliminary study, we have found that honey from manuka (Leptospermum scoparium) in New Zealand inhibits myeloperoxidase (MPO) activity. In this study, using a chromatographic technique, we isolated two active compounds for MPO-inhibition from manuka honey. One is methyl syringate (MSYR), and the other was identified as a novel glycoside of MSYR, methyl syringate 4-O-beta-D-gentiobiose, which has been named "leptosin" after the genus Leptospermum. The amount of the glycoside ranged from 0.2 to 1.2 mu mol/g honey. Leptosin was only found in honeys from the Oceania region, and abundantly in manuka honey including jelly bush honey from Leptospermum polygalifolium in Australia. Therefore, leptosin may be a good chemical marker for manuka honey. Interestingly, the concentration of leptosin in manuka honey was positively correlated with the unique manuka factor (UMF) value, which is expressed as phenol equivalents of its bactericidal activity., AMER CHEMICAL SOC, Apr. 2012, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60 (13), 3418 - 3423, doi;web_of_science

    Scientific journal

  • Antimutagenic and alpha-glucosidase inhibitory effects of constituents from Kaempferia parviflora

    Toshiaki Azuma; Shin-ichi Kayano; Yoko Matsumura; Yotaro Konishi; Yasuo Tanaka; Hiroe Kikuzaki

    The dichloromethane extract and ethyl acetate soluble fraction of Kaempferia parviflora showed antimutagenicity and alpha-glucosidase inhibitory activity. 7-Methoxyflavones were the major constituents of both extracts and demonstrated antimutagenic activity. In particular, 5,7-dimethoxyflavone (IC50 = 0.40 nmol/plate), 5,3'-dihydroxy-3,7,4'-trimethoxyflavone (0.40 nmol/plate), 3,5,7-trimethoxyflavone (0.42 nmol/plate) and 5-hydroxy-7-methoxyflavone (0.47 nmol/plate) showed potent antimutagenic activity. The results of structure-activity relationship studies suggest that methylation of the 5-hydroxy group enhanced activity. On the other hand, increasing the number of methoxy groups on the B ring caused a reduction in activity. In the case of an a-glucosidase inhibitory effect, 5,7,3',4'-tetramethoxyflavone exhibited the highest activity (IC50 = 20.4 mu M), followed by 5,7,4'-trimethoxyflavone (54.3 mu M) and 3,5,7,3',4'-pentamethoxyflavone (64.3 mu M). The results suggest that the a-glucosidase inhibitory activity was enhanced by the methoxy groups at the 5-, 3'- and 4'-position and was reduced by the methoxy group at the 3-position. (C) 2010 Elsevier Ltd All rights reserved., ELSEVIER SCI LTD, Mar. 2011, FOOD CHEMISTRY, 125 (2), 471 - 475, doi;web_of_science

    Scientific journal

  • 大量調理施設におけるエネルギー消費意識の実態調査と管理栄養士養成課程の学内給食実習におけるエネルギー消費量「見える化」の試み

    KIKUZAKI Hiroe

    Mar. 2011, 日本給食経営管理学会誌, 5 (1), 29-37

  • Inhibitory Activity of Pimenta dioica Extracts and Constituents on Recombinant Human Histidine Decarboxylase

    KIKUZAKI Hiroe; Yoko Nitta; Hiroe Kikuzaki; Hiroshi Ueno

    Jan. 2009, Food Chemistry, 113, 445-449

  • Phenolic Glycosides from Kaempferia parviflora

    KIKUZAKI Hiroe; Toshiaki Azuma; Yasuo Tanaka; Hiroe Kikuzak

    Oct. 2008, Phytochemistry, 69, 2743-2748

  • Phenolic glycosides from berries of Pimenta dioica

    Hiroe Kikuzaki; Yoshiko Miyajima; Nobuji Nakatani

    Four new phenolic glycosides, (27hydroxy-3-methoxy-5-allyl)phenyl beta-D-(6-O-E-sinapoyl)glucopyranoside (1), (1'R,5'R)-5-(5-carboxymethyl-2-oxocyclopentyl)-3Z-pentenyl beta-D-(6-O-galloyl)glucopyranoside (2), (S)-alpha-terpinyl [alpha-L-(2-O-galloyl)arabinofuranosyl]-(1 -> 6)-beta-D-glucopyranoside (3), and (R)-alpha-terpinyl [alpha-L-(2-O-galloyl)arabinofuranosyl]-(1 -> 6)-beta-D-glucopyranoside (4), were isolated from the berries of Pimenta dioica together with eight known flavonoids. The structures of 1-4 were elucidated on the basis of MS and NMR data and enzymatic hydrolysis. All four glycosides showed radical - scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals., AMER CHEMICAL SOC, May 2008, JOURNAL OF NATURAL PRODUCTS, 71 (5), 861 - 865, doi;web_of_science

    Scientific journal

  • Ecdysteroids act as inhibitors of calf skin collagenase and oxidative stress

    Robert Yawadio Nsimba; Hiroe Kikuzaki; Yotaro Konishi

    Three new phytoecdysteroids have been isolated from the seeds of Chenopodium quinoa and structurally identified as 20,26-dihydroxy, 28-methyl ecdysone, 20,26-dihydroxy, 24(28)-dehydro ecdysone, and 20-hydroxyecdysone 22-glycolate using serial chromatographic and spectroscopic methods. Both previously reported compounds and newly identified phytoecdysteroids were evaluated for their inhibitory effect on calf skin collagenase, as this enzyme is involved in aging skin diseases. Their effectiveness on scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, as well as in chelating the iron metal ions was also investigated. All isolated compounds demonstrated a higher potency to inhibit this matrix metalloproteinase and to chelate the iron ion, both with respect to the number of carbonyl groups bearing their carbon skeleton, suggesting that their mechanism of action involves their ability to coordinate both ions (either the zinc ion of the catalytic domain of collagenase or the iron ion), acting as an electron donor. The DPPH-scavenging ability was hydroxyl dependent. Satisfactory results obtained from the enzyme in vitro experiment were further supported by the gel electrophoresis. These results indicate that ecdysteroids might be considered as potent chemical agents to prevent or delay both collagenase-related skin damages and oxidative stress. (C) 2008 Wiley Periodicals, Inc., WILEY-BLACKWELL, 2008, JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 22 (4), 240 - 250, doi;web_of_science

    Scientific journal

  • Antioxidant activity of various extracts and fractions of Chenopodium quinoa and Amaranthus spp. seeds

    Robert Yawadio Nsimba; Hiroe Kikuzaki; Yotaro Konishi

    The antioxidant potency of various extracts and fractions from Chenopodium quinoa and Amaranthus sp. was evaluated using three established methods, specifically the DPPH scavenging activity, FRAP, and P-carotene bleaching assays. Satisfying results were obtained, which lead to expect the use of these seeds as health-promoting ingredients. The antioxidant activity was less correlated to the phenolics content suggesting that non-phenolic compounds might play major free radicals scavenging activity in studied plant materials. (c) 2007 Elsevier Ltd. All rights reserved., ELSEVIER SCI LTD, Jan. 2008, FOOD CHEMISTRY, 106 (2), 760 - 766, doi;web_of_science

    Scientific journal

  • Chemical constituents of cape aloe and their synergistic growth-inhibiting effect on Ehrlich ascites tumor cells

    Saeda Kametani; Akiko Kojima-Yuasa; Hiroe Kikuzaki; David Opare Kennedy; Mayumi Honzawa; Isao Matsui-Yuasa

    The constituents of cape aloe were investigated after a preliminary screening of the growth-inhibiting effect on Ehrlich ascites tumor cells (EATC) of several extracts of this plant. Ten compounds were isolated from the dichloromethane (CH2Cl2) extract that showed the strongest activity, and their structures were elucidated as aloe-emodin (1), p-hydroxybenzaldehyde (2), p-hydroxyacetophenone (3), pyrocatechol (4), 10-oxooctadecanoic acid (5), 10-hydroxyoctadecanoic acid (6), methyl 10-hydroxyoctadecanoate (7), 7-hydroxy-2,5-dimethylchromone (8), furoaloesone (9), and 2-acetonyl-8-(2-furoylmethyl)-7-hydroxy-5-methylchromone (10) based on MS and various NMR spectroscopic techniques. Compounds 2-7 were isolated for the first time from cape aloe. Compounds 4-7 and 10 showed a significant growth-inhibiting effect, and compound 1 exhibited a remarkable synergistic effect on compounds 8-10, which was not observed with the treatment by each compound alone on EATC. These results suggest that the strong growth-inhibiting effect of the CH2Cl2 extract was dependent not on one compound alone, but on the synergistic effect from the combination of compound 1 and the other compounds., TAYLOR & FRANCIS LTD, May 2007, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 71 (5), 1220 - 1229, doi;web_of_science

    Scientific journal

  • Food Components Inhibiting Recombinant Human Histidine Decarboxylase Activity

    KIKUZAKI Hiroe; Yoko Nitta; Hiroe Kikuzaki; Hiroshi Ueno

    Feb. 2007, J. Agric. Food Chem., 55 (2), 299-304

  • 給食経営管理実習における衛生管理に対するHACCP概念導入の教育効果について-作業過程における一般生菌数の推移と作業動線の実態調査から-

    KIKUZAKI Hiroe

    栄養士法一部改正以前の旧カリキュラムで実施した給食管理実習と改正後の新カリキュラムで実施した給食経営管理実習において、HACCP(危害分析重要管理点)概念を導入した衛生教育を行うことが実際の実習の場にどのような影響を与えるかを検証した。使用設備の一般生菌数の推移はHACCP教育の有無にかかわらず、作業の清潔度に応じた推移を示した。手指の一般生菌数の推移を調べた結果からはHACCP教育の導入による手指の洗浄改善効果は認めなかった。作業動線および温度管理状況に対しては、HACCP教育の効果が顕著に認めた。, 大阪市立大学大学院生活科学研究科, Mar. 2007, 生活科学研究誌, 5, 35 - 44, jamas_url;jamas_url

  • 特定給食施設(病院)における人件費率、食材料費の実態

    KIKUZAKI Hiroe

    Dec. 2006, 生活科学研究誌, 5, 45-50

  • Terpenoids and a diarylheptanoid from Zingiber ottensii

    Kayo Akiyama; Hiroe Kikuzaki; Takako Aoki; Akiko Okuda; Nordin H. Lajis; Nobuji Nakatani

    Four new terpenoids and a diarylheptanoid were isolated together with 16 known compounds from rhizomes of Zingiber ottensii. The structures of the new compounds were determined to be 1,10,10-trimethylbicyclo[7,4,0] tridecane-3,6-dione (1), (E)-14-hydroxy-15-norlabda-8(17), 12-dien-16-al (2), (E)-labda-8(17), 12,14-trien-15(16)-olide (3), (E)-14,-15,16- trinorlabda-8(17), 11-dien-13-oic acid (4), and rel-(3R, 5S)-3,5-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(3, 4-dihydroxyphenyl) heptane (5) by spectroscopic evidence., AMER CHEMICAL SOC, Nov. 2006, JOURNAL OF NATURAL PRODUCTS, 69 (11), 1637 - 1640, doi;web_of_science

    Scientific journal

  • Capsaicinol: Synthesis by allylic oxidation and its effect on TRPV1-expressing cells and adrenaline secretion in rats

    Kenji Kobata; Takahito Iwasawa; Yusaku Iwasaki; Akihito Morita; Yuichi Suzuki; Hiroe Kikuzaki; Nobuji Nakatani; Tatsuo Watanabe

    Capsaicinol is an ingredient of hot red pepper. In this study, we developed a novel method for capsaicinol synthesis and examined capsaicinol's physiological effects on capsaicin receptor (TRPV1)-related actions. Allylic oxidation of capsaicin by palladium acetate (Pd(OAc)(2)) resulted in the formation of (+/-)-capsaicinol acetate at a 7.2% yield in a single step. The effectiveness of (+/-)-capsaicinol in TRPV1 activation (EC50 = 1.1 mu M) was found to be weaker than that of capsaicin (EC50 = 0.017 mu M), whereas the efficacy of (+/-)-capsaicinol reached 15% of that of capsaicin. Intravenous administration of (+/-)-capsaicinol in anesthetized rats dose-dependently enhanced adrenaline secretion from the adrenal gland. The response to a 5mg/kg-dose of (+/-)-capsaicinol was comparable to that of a 0.05 mg/kg-dose of capsaicin. The relative pungency of capsaicinol to capsaicin was coincident with the relative effectiveness in. inducing these TRPV1-related actions., TAYLOR & FRANCIS LTD, Aug. 2006, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70 (8), 1904 - 1912, doi;web_of_science

    Scientific journal

  • Chemical Constituents of Piper retrofractum Vahl and Their Antioxidant and Radical Scavenging Activities

    KIKUZAKI Hiroe; Saeda Kametani; Hiroe Kikuzaki; Mayumi Honzawa; Nobuji Nakatani

    Dec. 2005, ITE Letters on Batteries, New Technologies & Medicine, 6, 566-573

  • Antioxidative constituents of Etlingera elatior

    H Mohamad; NH Lajis; F Abas; AM Ali; MA Sukari; H Kikuzaki; N Nakatani

    Phytochemical studies on the rhizomes of Etlingera elatior have resulted in the isolation of 1,7-bis(4-hydroxyphenyl)-2,4,6-heptatrienone (1), demethoxycurcumin (2), 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (3), 16-hydroxylabda-8(17),11,13-trien-15,16-olide (4), stigmast-4-en-3-one, stigmast-4-ene-3,6-dione, stigmast-4-en-6beta-ol-3-one, and 5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol. Compounds 1 and 4 are new, and their structures were elucidated by analysis of spectroscopic data. Diarylheptanoids 1-3 were found to inhibit lipid peroxidation in a more potent manner than alpha-tocopherol., AMER CHEMICAL SOC, Feb. 2005, JOURNAL OF NATURAL PRODUCTS, 68 (2), 285 - 288, doi;web_of_science

    Scientific journal

  • Screening of Spice and Herb Extracts for Cytotoxic Activity against Ehrlich Ascites Tumor Cells and DPPH Radical Scavenging Activity

    KIKUZAKI Hiroe; Saeda Kametani; Isao Matsui-Yuasa; Akiko Kojima-Yuasa; Tsutomu Arimura; David Opare Kennedy; Mayumi Honzawa; Hiroe Kikuzaki; Nobuji Nakatani

    Dec. 2004, ITE Letters on Batteries, New Technologies & Medicine, 5, 67-75

  • DPPH free radical scavenger components from the fruits of Alpinia rafflesiana Wall. ex. Bak. (Zingiberaceae)

    H Mohamad; F Abas; D Permana; NH Lajis; AM Ali; MA Sukari; TYY Hin; H Kikuzaki; N Nakatani

    The methanol extract of the dried ripe fruits of Alpinia rafflesiana was investigated for its DPPH free radical scavenger constituents. 2',3',4',6'-Tetrahydroxychalcone (7), which has never been isolated from natural sources was found to be most active as a DPPH free radical scavenger with the IC50 value of 55 mum. Other known compounds isolated from this species include 5,6-dehydrokawain (1), flavokawin B (2), 1,7-diphenyl-5-hydroxy-6-hepten-3- one (3), (-)-pinocembrin (4), cardamonin (5) and (-)-pinostrobin (6). The DPPH free radical scavenger compounds were detected using TLC autographic analysis. The percentage inhibition of DPPH free radical scavenging activity was measured on isolates (5-7) using colorimetric analysis., VERLAG Z NATURFORSCH, Nov. 2004, ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, 59 (11-12), 811 - 815, web_of_science

    Scientific journal

  • Constituents of the Leaves of Peucedanum japonicum Thunb. and Their Biological Activity

    KIKUZAKI Hiroe; Masashi Hisamoto; Hiroe Kikuzaki; Nobuji Nakatani

    Apr. 2004, J. Agric. Food Chem., 52, 445-450

  • A new bipyrrole and some phenolic constituents in prunes (Prunus domestica L.) and their oxygen radical absorbance capacity (ORAC)

    S Kayano; H Kikuzaki; T Ikami; T Suzuki; T Mitani; N Nakatani

    Isolation and structural elucidation of prune constituents were performed and total 10 compounds were determined by NMR and MS analyses. A novel compound was identified to be 2-(5-hydroxymethyl-2',5'dioxo-2',3',4',5'-tetrahydro-l'H-1,3'-bipyrrole)carbalde- hyde, and 7 phenolic compounds were isolated from prunes for the first time. In addition, antioxidant activity of them was evaluated on the basis of the oxygen radical absorbance capacity (ORAC)., TAYLOR & FRANCIS LTD, Apr. 2004, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68 (4), 942 - 944, web_of_science

    Scientific journal

  • 大量調理に使用する粉末出しのミネラル含量-学校給食から-

    KIKUZAKI Hiroe

    Apr. 2004, 食生活研究, 24, 40-46

  • Abscisic Acid Related Compounds and Lignans in Prunes (Prunus domestica L.) and Their Oxygen Radical Absorbance Capacity (ORAC)

    KIKUZAKI Hiroe; Hiroe Kikuzaki; Shin-ichi Kayano; Naoko Fukutsuka; Asuka Aoki; Kumi Kasamatsu; Yuka Yamasaki; Takahiko Mitani; Nobuji Nakatani

    Mar. 2004, J. Agric. Food Chem., 52, 344-349

  • Antioxidant properties of gingerol related compounds from ginger

    Y Masuda; H Kikuzaki; M Hisamoto; N Nakatani

    Ginger (Zingiber officinale Roscoe) shows an antioxidant activity, and we have been engaging to determine the structures of more than 50 antioxidants isolated from the rhizomes of ginger. The isolated antioxidants are divided into two groups; gingerol related compounds and diarylheptanoids. In this study, structure-activity relationship of gingerol related compounds was evaluated. Gingerol related compounds substituted with an alkyl group bearing 10-, 12- or 14-carbon chain length were isolated from the dichloromethane extract of rhizomes using repeated chromatographic techniques. The antioxidant activities of these compounds were evaluated by the following measurements; 1) 1, 1 -diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2) inhibitory effect on oxidation of methyl linoleate under aeration and heating by the Oil Stability Index (OSI) method, and 3) inhibitory effect on oxidation of liposome induced by 2,2 -azobis(2-amidinopropane)dihydrochloride (AAPH). These results suggested that the substituents on the alkyl chain might contribute to both radical scavenging effect and inhibitory effect of autoxidation of oils, while inhibitory effects against the AAPH-induced peroxidation of liposome was somewhat influenced by the alkyl chain length; the antioxidant activity might be due to not only radical scavenging activity of antioxidants but also their affinity of the antioxidants to the substrates., IOS PRESS, 2004, BIOFACTORS, 21 (1-4), 293 - 296, web_of_science

    Scientific journal

  • Antioxidative polyphenols from berries of Pimenta dioica

    Y Miyajima; H Kikuzaki; M Hisamoto; N Nakatani

    The ethyl acetate-soluble part of allspice, berries of Pimenta dioica, showed strong antioxidant activity and radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazy] (DPPH) radical. From the ethyl acetate-soluble part, two new compounds, 5-galloyloxy-3,4-dihydroxypentanoic acid and 5-(5-carboxymethyl-2-oxocyclopentyl)-3Z-pentenyl 6-O-galloyl-beta-D-glucoside were isolated together with 11 known polyphenols by repeated column chromatography. Their structures were elucidated on the basis of MS and various NMR spectroscopic data.All isolated compounds were evaluated for antioxidative effects on oxidation of methyl linoleate under aeration and heating, and on peroxidation of liposome induced by 2,1-azobis-(2-amidinopropane)dihydrocloride (AAPH) as a water-soluble initiator along with their radical -scavenging activity against DPPH. Quercetin and its glycosides showed remarkable activity for scavenging DPPH radical and inhibiting peroxidation of liposome. Two new compounds also exhibited strong DPPH radical -scavenging activity and inhibitory effect on the peroxidation of liposome as myricetin., IOS PRESS, 2004, BIOFACTORS, 21 (1-4), 301 - 303, web_of_science

    Scientific journal

  • Antioxidant properties of prunes (Prunus domestica L.) and their constituents

    SI Kayano; H Kikuzaki; NF Yamada; A Aoki; K Kasamatsu; Y Yamasaki; T Ikami; T Suzuki; T Mitani; N Nakatani

    Prunes contain large amounts of phenolics and show high antioxidant activity. The aim of this study is to clarify the contents of caffeoylquinic acid (CQA) isomers, and to estimate the contribution of these isomers to the antioxidant activity of prunes. Furthermore, structural elucidation and evaluation of antioxidant activity of prune components were also performed. CQA isomers in prunes were quantified by HPLC analysis, and it has become apparent that prunes contain relatively high amount of 4-O-caffeoylquinic acid. The contribution of CQA isomers to the antioxidant activity of prunes was revealed to be 28.4% on the basis of oxygen radical absorbance capacity (ORAC); hence, it was indicated that residual ORAC is dependent on unknown antioxidant components. Total 28 compounds were isolated and their structures were elucidated by NMR and MS analyses. Four abscisic acid related compounds, a chromanon, and a bipyrrole were novel. Each CQA isomer in prunes showed high antioxidant activities when measured by the oil stability index (OSI) method, O-2(-) scavenging activity, and ORAC. Other isolated compounds such as hydroxycinnamic acids, benzoic acids, coumarins, lignans, and flavonoid showed high ORAC values. Furthermore, a novel chromanon indicated a remarkable synergistic effect on ORAC of CQA isomers., IOS PRESS, 2004, BIOFACTORS, 21 (1-4), 309 - 313, web_of_science

    Scientific journal

  • Antioxidant and antitumor promoting activities of the flavonoids from Hedychium thyrsiforme

    Jasril; LY Mooi; NH Lajis; AM Ali; MA Sukari; AA Rahman; AG Othman; H Kikuzaki; N Nakatani

    Five flavonoids, including 3,7,4'-trimethoxy-5-hydroxyflavone (1), 3,4'-dimethoxy-5,7-dihydroxyflavone (2), 5,7,4'-trimethoxy-3-hydroxyflavone (3), 3,5,7,4'-tetramethoxyflavone (4), and 7,4'-dimethoxy-3,5-dihydroxyflavone (5), were isolated from the rhizome of Hedychium thyrsiforme and assayed for antioxidant and antitumor promoting activities. The antioxidant assays showed that 5,7,4'-trimethoxy-3-hydroxyflavone, 7,4'-dimethoxy-3,5-dihydroxyflavone and 3,4'-dimethoxy-5,7-dihydroxyflavone had strong activities. Only two compounds, 5,7,4'-trimethoxy-3-hydroxyflavone and 7,4'-dimethoxy-3,5-dihydroxyflavone, were found to be strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavengers with fifty percent inhibition concentration (IC50) values of 92 and 119 muM, respectively. Antitumor promoting assays indicated that all the flavonoids showed strong inhibition activity towards Epstein-Barr virus (EBV) activation in Raji cells., SWETS ZEITLINGER PUBLISHERS, Oct. 2003, PHARMACEUTICAL BIOLOGY, 41 (7), 506 - 511, web_of_science

    Scientific journal

  • Comparison of antioxidative properties of carbazole alkaloids from Murraya koenigii leaves

    Y Tachibana; H Kikuzaki; NH Lajis; N Nakatani

    A new dimeric carbazole alkaloid, 8,10'-[3,3',11,11'-tetrahydro-9,9'-dihydroxy-3,3',5,8'-tetramethyl-3,3'-bis(4-methyl-3-pentenyl)]bipyrano[3,2-a]carbazole (12), was isolated from the CH2Cl2 extract of Murraya koenigii together with six known carbazole alkaloids, koenimbine (6), O-methylmurrayamine A (7), O-methylmahanine (8), isomahanine (9), bismahanine (10), and bispyrayafoline (11). Their structures were determined on the basis of H-1 and C-13 NMR spectroscopic and mass spectrometric (MS) data. The antioxidative properties of 12 carbazole alkaloids isolated from leaves of M. koenigii were evaluated on the basis of the oil stability index together with their radical scavenging ability against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. On the basis of the lag time to reach a steady state, the 12 carbazoles were classified into three groups. It is suggested that an aryl hydroxyl substituent on the carbazole rings plays a role in stabilizing the thermal oxidation and rate of reaction against DPPH radical., AMER CHEMICAL SOC, Oct. 2003, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 51 (22), 6461 - 6467, doi;web_of_science

    Scientific journal

  • Quantification of Quinic Acid in Prunes (Prunus domestica L.) by HPLC Analysis

    KIKUZAKI Hiroe; Shin-ichi Kayano; Takahiko Mitani; Hirotaka Yamamoto; Takao Ikami; Hiroe Kikuzaki; Tomoo Suzuki; Nobuji Nakatani

    Oct. 2003, ITE Letters on Batteries, New Technologies & Medicine, 4, 639-644

  • Antioxidant compounds from the leaves of Peucedanum japonicum Thunb

    M Hisamoto; H Kikuzaki; H Ohigashi; N Nakatani

    Seventeen compounds were isolated from the n-butanol soluble fraction of the leaves of Peucedanum japonicum Thunb. On the basis of MS and various NMR spectroscopic techniques, the structures of the isolated compounds were determined as isoquercitrin (1), rutin (2), 3-O-caffeoylquinic acid (3), 4-O-caffeoylquinic acid (4), 5-O-caffeoylquinic acid (5), cnidioside A (6), praeroside II (7), praeroside III (8), apterin (9), esculin (10), (R)-peucedanol (11), (R)-peucedanol 7-O-beta-D-glucopyranoside (12), L-tryptophan (113), uracil (114), guanosine (115), uridine (116), and thymidine (117). All compounds except 11 and 12 were isolated for the first time from P. japonicum. Several isolated compounds were quantified by high-performance liquid chromatography analysis. In addition, all isolated compounds were examined for radical scavenging on 1,1-diphenyl-2-picrylhydrazyl radical and for inhibition of oxidation of liposome induced by 2,2'-azobis(2-amidinopropane)dihydrochloride. Compounds 2-5 were found to be the major potent constituents, which contribute to the antioxidant activity of P. japonicum leaves., AMER CHEMICAL SOC, Aug. 2003, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 51 (18), 5255 - 5261, doi;web_of_science

    Scientific journal

  • Conformational Isomers of 3-O-Caffeoylquinic Acid Isolated from Prunes (Prunus domestica L.) and Their Antioxidant Activity

    KIKUZAKI Hiroe; Shin-ichi Kayano; Hiroe Kikuzaki; Takahiko Mitani; Nobuji Nakatani

    Aug. 2003, ITE Letters on Batteries, New Technologies & Medicine, 4, 478-484

  • Lack of Inhibitory Effects of Coumaperine from Pepper on the Promotion Stage of Chemical Hepatocarcinogenesis in the Rat

    KIKUZAKI Hiroe; Mitsuaki Kitano; Yutaka Ariki; Jutaro Wada; Yoo Tanabe; Hiroe Kikuzaki; Nobuji Nakatani; Hideki Wanibuchi; Keiichirou Morimura; Takayoshi Hidaka; Shoji Fukushima

    Aug. 2003, J. Toxicol. Pathol., 16, 161-164

  • The Antioxidative Components from Alpinia nutans

    KIKUZAKI Hiroe; M. Habsah; N.H. Lajis; A.M. Ali; M.A. Sukari; Y.Y. Hin; H. Kikuzaki; N. Nakatani

    Jul. 2003, Pharmaceutical Biology, 41, 7-9

  • Quantitative Evaluation of Antioxidant Components in Prunes (Prunus domestica L.)

    KIKUZAKI Hiroe; Shin-ichi Kayano; Naoko Fukutsuka Yamada; Tomoo Suzuki; Takao Ikami; Koichiro Shioaki; Hiroe Kikuzaki; Takahiko Mitani; Nobuji Nakatani

    May 2003, J. Agric. Food Chem., 51, 1480-1485

  • 沖縄産香辛植物の食中毒菌増殖抑制作用

    KIKUZAKI Hiroe

    Apr. 2003, 生活科学研究誌, 1, 11-16

  • Sphingolipid and other constituents from almond nuts (Prunus amygdalus Batsch)

    SM Sang; H Kikuzaki; K Lapsley; RT Rosen; N Nakatani; CT Ho

    One sphingolipid, 1-O-beta-D-glucopyranosyl-(2S,3R,4E,8Z)-2-[(2R)-2-hydroxyhexadecanoylamino]-4,8-octadecadiene-1,3-diol, and four other constituents, beta-sitosterol, daucosterol, uridine, and adenosine, have been isolated from the nuts of almond (Prunus amygdalus). Complete assignments of the proton and carbon chemical shifts for the sphingolipid were accomplished on the basis of high-resolution 1D and 2D NMR. data. All of these compounds are being reported from almond nuts (P. amygdalus) for the first time., AMER CHEMICAL SOC, Jul. 2002, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50 (16), 4709 - 4712, doi;web_of_science

    Scientific journal

  • Antifungal Garcinia Acid Esters from the Fruits of Garcinia atroviridis

    KIKUZAKI Hiroe; Mukram M. Mackeen; Abdul Manaf Ali; Nordin Hj. Lajis; Kazuyoshi Kawazu; Hiroe Kikuzaki; Nobuji Nakatani

    May 2002, Z. Naturforsch, 57c, 291-295

  • Antixoidant Activity of Prune (Prunus domestica L.) Constituents and a New Synergist

    KIKUZAKI Hiroe; Shin-ichi Kayano; Hiroe Kikuzaki; Naoko Fukutsuka; Takahiko Mitani; Nobuji Nakatani

    May 2002, J. Agric. Food Chem., 50, 3708-3712

  • Antioxidant activity of chemical components from sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index method

    K Miura; H Kikuzaki; N Nakatani

    A new abietane diterpenoid, 12-O-methyl carnosol (2), was isolated from the leaves of sage (Salvia officinalis L.), together with 11 abietane diterpenoids, 3 apianane terpenoids, 1 anthraquinone, and 8 flavonoids. Antioxidant activity of these compounds along with 4 flavonoids isolated from thyme (Thymus vulgaris L.) was evaluated by the oil stability index method using a model substrate oil including methyl linoleate in silicone oil at 90 degreesC. Carnosol, rosmanol, epirosmanol, isorosmanol, galdosol, and carnosic acid exhibited remarkably strong activity, which was comparable to that of alpha-tocopherol. The activity of miltirone, atuntzensin A, luteolin, 7-O-methyl luteolin, and eupafolin was comparable to that of butylated hydroxytoluene. The activity of these compounds was mainly due to the presence of ortho-dihydroxy groups. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of these compounds showed the similar result., AMER CHEMICAL SOC, Mar. 2002, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50 (7), 1845 - 1851, doi;web_of_science

    Scientific journal

  • Antioxidant properties of ferulic acid and its related compounds

    H Kikuzaki; M Hisamoto; K Hirose; K Akiyama; H Taniguchi

    Antioxidant activity of 24 ferulic acid related compounds together with 6 gallic acid related compounds was evaluated using several different physical systems as well as their radical scavenging activity. The radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) decreased in the order caffeic acid > sinapic acid > ferulic acid > ferulic acid esters > p-coumaric acid. In bulk methyl linoleate, test hydroxycinnamic acids and ferulic acid esters showed antioxidant activity in parallel with their radical scavenging activity. In an ethanol-buffer solution of linoleic acid, the activity of test compounds was not always associated with their radical scavenging activity. Ferulic acid was most effective among the tested phenolic acids. Esterification of ferulic acid resulted in increasing activity. The activity of alkyl ferulates was somewhat influenced by the chain length of alcohol moiety. When the inhibitory effects of alkyl ferulates against oxidation of liposome induced by AAPH were tested, hexyl, octyl, and 2-ethyl-1-hexyl ferulates were more active than the other alkyl ferulates. Furthermore, lauryl gallate is most effective among the tested alkyl gallates. These results indicated that not only the radical scavenging activity of antioxidants, but also their affinity with lipid substrates, might be important factors in their activity., AMER CHEMICAL SOC, Mar. 2002, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50 (7), 2161 - 2168, doi;web_of_science

    Scientific journal

  • New flavonol-phenylbutadiene adducts from the leaves of Alpinia flabellata

    H Kikuzaki; S Tesaki

    Two new flavonol-phenylbutadiene adducts, rel-5-hydroxy-7,4-dimethoxy-2"S-(2,4,5-trimethoxy-E-styryl)tetrahydrofuro[4"R,5"R:2,3]flavanonol (1) and rel-5-hydroxy-7,4'-dimethoxy-3"S-(2,4,5-trimethoxy-E-styryl)tetrahydrofuro[4"R,5"R:2,3]flavanonol (2), were isolated from the leaves of Alpinia flabellata, along with three known compounds, 2,4,5-trimethoxybenzoic acid, 2,4,5-trimethoxycinnamic acid, and 5-hydroxy-3,7,4'-trimethoxyflavone. The structures of 1 and 2 were determined by spectroscopic interpretation., AMER CHEMICAL SOC, Mar. 2002, JOURNAL OF NATURAL PRODUCTS, 65 (3), 389 - 391, doi;web_of_science

    Scientific journal

  • Antibacterial Activity of Essential Oils and Oleoresins of Spices and Herbs against Pathogens Bacteria in Upper Airway Respiratory Tract

    KIKUZAKI Hiroe; Yasuo Tanaka; Hiroe Kikuzaki; Nobuji Nakatani

    Feb. 2002, Jpn. J. Food Chem., 9, 67-76

  • Antioxidant Activity of Tropical Plant Leaves Traditionally Used for Food Preservation in Okinawa

    KIKUZAKI Hiroe; Masashi Hisamoto; Hiroe Kikuzaki; Sigetomo Yonemori; Nobuji Nakatani

    Jan. 2002, ITE Letters on Batteries, New Technologies & Medicine, 3, 63-68

  • Apianane terpenoids from Salvia officinalis

    K Miura; H Kikuzaki; N Nakatani

    Three apianane terpenoids, rel-(5S, 6S, 7S, 10R, 12S, 13R)-7-hydroxyapiana-8,14-diene-11,16-dion-(22,6)-olide, rel-(5S, 6S, 7R, 10R, 12S, 13R)-7-hydroxyapiana-8,14-diene-11,16-dion-(22,6)-olide and rel-(5S, 6S, 7S, 10R, 12R, 13S)-7-hydroxyapiana-8,14diene-11,16-dion-(22,6)-olide were isolated from the leaves of Salvia officinalis, together with 15 known compounds, salvigenin, lupeol, beta -sitosterol, stigmasterol, physcion, carnosol, rosmadial, rosmanol, epirosmanol, isorosmanol, columbaridione, atuntzensin A, miltirone, carnosic acid and 12-O-methyl carnosic acid. Their structures were established on the basis of spectral evidence. (C) 2001 Published by Elsevier Science Ltd., PERGAMON-ELSEVIER SCIENCE LTD, Dec. 2001, PHYTOCHEMISTRY, 58 (8), 1171 - 1175, web_of_science

    Scientific journal

  • Antioxidative activity of carbazoles from Murraya koenigii leaves

    Y Tachibana; H Kikuzaki; NH Lajis; N Nakatani

    The antioxidative properties of the leaves extracts of Murraya koenigii using different solvents were evaluated based on the oil stability index (OSI) together with their radical scavenging ability against 1-1-diphenyl-2-picrylhydrazyl (DPPH). The methylene chloride (CH2Cl2) extract and the ethyl acetate (EtOAc) soluble fraction of the 70% acetone extract significantly prolonged the OSI values comparable to those of alpha -tocopherol and BHT. Five carbazole alkaloids were isolated from the CH2Cl2 extract and their structures were identified to be euchrestine B (1), bismurrayafoline E (2), mahanine (3), mahanimbicine (4), and mahanimbine (5) based on H-1 and C-13 NMR and mass (MS) spectral data. The OSI value of carbazoles at 110 degreesC decreased in the order 1 and 3 > alpha -tocopherol > BHT > 2 > 4, 5 and control. It is' assumed that compounds 1 and 3 contributed to the high OSI. value of the CH2Cl2 extract of M. koenigii. The DPPH radical scavenging activity for these carbazoles-was in the order ascorbic acid > 2 > 1, 3 and alpha -tocopherol > BHT > 4 and 5., AMER CHEMICAL SOC, Nov. 2001, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 49 (11), 5589 - 5594, doi;web_of_science

    Scientific journal

  • Furanosesquiterpenoids of Commiphora myrrha

    KIKUZAKI Hiroe; Nanqun Zhu; Hiroe Kikuzaki; Shuqun Sheng; Shengmin Sang; Mohamed M. Rafi; Mingfu Wang; Nobuji Nakatani; Robert S. DiPaola; Robert T. Rosen; Chi-Tang Ho

    Nov. 2001, J. Nat. Prod., 64, 1460-1462

  • Antibacterial compounds of licorice against upper airway respiratory tract pathogens

    Y Tanaka; H Kikuzaki; S Fukuda; N Nakatani

    The antibacterial activity of compounds obtained from licorice was measured against upper airway respiratory tract bacteria such as Streptococcus pyogenes. Haemophilus influenzae and Moraxella catarrhalis. Among the tested compounds, licoricidin exhibited the highest activity against all tested microorganisms with an MIC of 12.5,mug/mL. Three coumarin derivatives, glycyrol, glycyrin and glycycoumarin also showed antibacterial activity., CENTER ACADEMIC PUBL JAPAN, Jun. 2001, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 47 (3), 270 - 273, web_of_science

    Scientific journal

  • Ecdysteroids of Quinoa seeds (Chenopodium quinoa Willd.)

    N Zhu; H Kikuzaki; BC Vastano; N Nakatani; MV Karwe; RT Rosen; CT Ho

    Quinoa (Chenopodium quinoa) is a hardy and nutritious Latin American pseudo-cereal. Studies on the seeds led to the isolation of five ecdysteroids using column chromatography. Their structures were determined as ecdysterone, makisterone A, 24-epi-makisterone A, 24(28)-dehydromakisterone A. and 20,26-dihydroxyecdysone by spectroscopic methods. This study demonstrates that quinoa seeds are a source of ecdysteroids, which were reported to be molting hormones in insects., AMER CHEMICAL SOC, May 2001, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 49 (5), 2576 - 2578, web_of_science

    Scientific journal

  • New constituents of the leaves of Alpinia flabellata

    S Tesaki; H Kikuzaki; S Yonemori; N Nakatani

    As a part of our studies on the constituents of plants belonging to the family Zingiberaceae,(1-5) we have focused on Alpinia flabellata Ridley, which is a rare species in Japan. The leaves of this plant have been used as a wrapping and flavoring material for foods on the islands of Iriomote and Ishigaki, Okinawa, which is the southernmost part of Japan. Only two studies on the isolation of several phenylbutenoids from the rhizomes of A. flabellata have been reported.(6.7) We have reported previously the isolation and structural elucidation of three phenylbutanoid dimers from the leaves of A. flabellatas(8) and now describe the isolation and characterization of a new phenylbutenal (1) and a novel labdane diterpene (2) adducted by a phenylbutenoid., AMER CHEMICAL SOC, Apr. 2001, JOURNAL OF NATURAL PRODUCTS, 64 (4), 515 - 517, web_of_science

    Scientific journal

  • 1,1-diphenyl-2-picrylhydrazyl radical-scavenging active compounds from greater cardamom (Amomum subulatum Roxb.)

    H Kikuzaki; Y Kawai; N Nakatani

    Constituents of the fruits of greater cardamom (Amomum subulatum) were fractionated into three fractions, the dichloromethane extract, and the ethyl acetate-soluble and water-soluble fractions of the 70% aqueous acetone extract, The ethyl acetate-soluble fraction showed a high radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH). Four compounds were isolated from the ethyl acetate-soluble fraction, and their structures were ascribed to protocatechualdehyde (1), protocatechuic acid (2), 1,7-bis(3,4-dihydroxyphenyl)hepta-4E, 6E-dien-3-one (3) and 2,3, 7-trihydroxy-5-(3,4-dihydroxy-E-styryl)-6,7,8,9 -tetrahydro-5H-benzocycloheptene (4) on the basis of spectroscopic evidence. This is the first isolation of these compounds from greater cardamom. In particular, 4 was a new type of cyclic diarylheptanoid. DPPH radical-scavenging activity of these compounds was measured by colorimetric analysis. Compounds 1 and 3 showed stronger activity than such natural antioxidants as alpha -tocopherol and L-ascorbic acid. Compounds 2 and 4 were comparable to alpha -tocopherol and L-ascorbic acid., CENTER ACADEMIC PUBL JAPAN, Apr. 2001, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 47 (2), 167 - 171, web_of_science

    Scientific journal

  • Establishment of a model substrate oil for antioxidant activity assessment by Oil Stability Index method

    N Nakatani; Y Tachibana; H Kikuzaki

    A model substrate oil using methyl linoleate was established for the determination of the antioxidant activity by Oil Stability Index (OSI) method. OSI values for methyl linoleate with different concentrations (5-100%) in silicone oil were measured at different temperatures (70-120 degreesC), As the temperature increased, the OSI value decreased in each concentration of methyl linoleate. Optimal temperature and concentration of methyl linoleate were established. The effect Of concentration of antioxidants, alpha -tocopherol, and butylated hydroxytoluene on OSI values for 10% methyl linoleate model oil was measured at 90, 100, 110, and 120 degreesC, The logarithmic reiationship between temperature and OSI using model substrate oil was similar to that of soybean oil. Furthermore, application of some spice extracts to this model oil system was carried out to give results that compared well with those available in the literature. Thus, the procedure using methyl linoleate-silicone oil as a model substrate oil is available for evaluating the antioxidant activity by the OSI method., AMER OIL CHEMISTS SOC A O C S PRESS, Jan. 2001, JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 78 (1), 19 - 23, web_of_science

    Scientific journal

  • Phenylbutanoid dimers from the leaves of Alpinia flabellata

    H Kikuzaki; S Tesaki; S Yonemori; N Nakatani

    Three phenylbutanoid dimers, cis- and trans-1-(2,4,5-trimethoxy-E-styryl)-2-(2,4,5-trimethoxy-Z-styryl)cyclobutane and 1,2- bis(2,4,5-trimethoxy-Z-styryl)- cyclobutane, were isolated from the leaves of Alpinia flabellata Ridley, together with three known compounds (2,4,5-trimethoxybenzaldehyde, 2,4,5-trimethoxycinnamaldehyde and 3,5-dihydroxy-7,4'-dimethoxyflavone). The structures of these compounds were determined by spectroscopic analysis. (C) 2001 Elsevier Science Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD, Jan. 2001, PHYTOCHEMISTRY, 56 (1), 109 - 114, web_of_science

    Scientific journal

  • Antibacterial Activity of Phenolic Constituents Isolated from Alpinia flabellata and Their Regulating Effect on Ovalbumin Transport in Human Intestinal Caco-2 Cells

    KIKUZAKI Hiroe; Shoko Tesaki; Hiroe Kikuzaki; Soichi Tanabe; Michiko Watanabe; Nobuji Nakatani

    Jan. 2001, ITE Letters on Batteries, New Technologies & Medicine, 2, 106-111

  • Identification, quantitative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L.)

    N Nakatani; S Kayano; H Kikuzaki; K Sumino; K Katagiri; T Mitani

    Neochlorogenic acid (3-CQA) and cryptochlorogenic acid (4-CQA), isolated from prune (Prunus domestica L.), were identified by NMR and MS analyses. In addition, the quantity of chlorogenic acid isomers in prune were measured by HPLC. These isomers, 3-CQA, 4-CQA, and chlorogenic acid (5-CQA), were contained in the ratio 78.7:18.4:3.9, respectively. 4-CQA was identified and quantified in prune for the first time, and relatively high amounts of this isomer were characteristic. Antioxidative activities of the chlorogenic acid isomers, such as scavenging activity on superoxide anion radicals and inhibitory effect against oxidation of methyl linoleate, were also evaluated. Each isomer showed antioxidative activities which were almost the same., AMER CHEMICAL SOC, Nov. 2000, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 48 (11), 5512 - 5516, doi;web_of_science

    Scientific journal

  • Antibacterial Activity of Aliphatic Long Chain Compounds against Upper Airway Respiratory Tract Bacteria

    KIKUZAKI Hiroe; Yasuo Tanaka; Seiji Fukuda; Hiroe Kikuzaki; Nobuji Nakatani

    Sep. 2000, ITE Letters on Batteries, New Technologies & Medicine, 1, 777-780

  • アガリクス(Agaricus blazei)子実体の抗酸化成分および多糖の研究

    KIKUZAKI Hiroe

    Sep. 2000, 微量栄養素研究, 17, 83-90

  • Chemopreventive effects of coumaperine from pepper on the initiation stage of chemical hepatocarcinogenesis in the rat

    M Kitano; H Wanibuchi; H Kikuzaki; N Nakatani; S Imaoka; K Funae; S Hayashi; S Fukushima

    This study was designed to investigate the chemopreventive action of three natural products, coumaperine, aurapten and an extract from rosemary, against the initiation stage of rat hepatocarcinogenesis. Coumaperine has been isolated from white pepper as a naturally occurring antioxidative agent, but its potential modifying effects on carcinogenesis remain unclear. In experiment 1, a modification of the model developed by Tsuda et al. was applied, with assessment of numbers and areas of induced glutathione S-transferase placental form (GST-P)-positive hepatocellular foci in male F344 rats. Coumaperine, aurapten and the extract from rosemary were administered i.g. at 190 mg/kg/day once daily for 5 days with initiation by diethylnitrosamine (DEN) on day 4 (20 mg/kg, i.p.). Numbers and areas of GST-P-positive foci in each group given test chemicals tended to be decreased as compared to the vehicle control group values, significance being achieved for number with coumaperine. Experiment 2 was planned to investigate the mechanism of the inhibitory effects of coumaperine. Livers at 8 h after initiation by DEN were examined with coumaperine administered at 100 mg/kg/day once daily for 3 days. Proliferating cell nuclear antigen (PCNA)positive cells tended to be decreased as compared to the vehicle control, but no effects on apoptosis or cytochrome P-450 (CYP) 2E1 expression were apparent. Our results suggest that coumaperine provides protection against initiation of hepatocarcinogenesis, and that this is related to inhibition of cell proliferation., BUSINESS CENTER ACADEMIC SOCIETIES JAPAN, Jul. 2000, JAPANESE JOURNAL OF CANCER RESEARCH, 91 (7), 674 - 680, web_of_science

    Scientific journal

  • Novel glycosides from noni (Morinda citrifolia)

    Mingfu Wang; Hiroe Kikuzaki; Yi Jin; Nobuji Nakatani; Nanqun Zhu; Katalin Csiszar; Charles Boyd; Robert T. Rosen; Geetha Ghai; Chi-Tang Ho

    Three new glycosides were isolated from the fruits of noni (Morinda citrifolia). Their structures were determined to be 6-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose (1), 6-O-(β-D-glucopyranosyl)-1-O-hexanoyl-β-D-glucopyranose (2), and 3-methylbut-3-enyl 6-O-β-D-glucopyranosyl-β-D-glucopyranoside (3) using MS and NMR methods., 2000, Journal of Natural Products, 63 (8), 1182 - 1183, doi;pubmed

    Scientific journal

  • Constituents of Amomum tsao-ko and their radical scavenging and antioxidant activities

    TS Martin; H Kikuzaki; M Hisamoto; N Nakatani

    Constituents of the fruit of Amomum tsao-ko were investigated following a preliminary screening of the antioxidant activity of several extracts of the fruit of this plant that showed that the dichloromethane extract and the ethyl acetate-soluble and water-soluble fractions of the 70% aqueous acetone extract had higher activity than alpha-tocopherol and butylated hydroxytoluene (BHT). Eleven compounds were isolated from the ethyl acetate-soluble fraction, and their structures were elucidated as (+)-hannokinol (1), meso-hannokinol (2), (+)-epicatechin (3), (-)-catechin (4), beta-sitosterol (5), beta-sitosterol 3-O-glucoside (6), 2,6-dimethoxyphenol (7), protocatechualdehyde (8), protocatechuic acid (9), vanillic acid (10), and beta-hydroxybenzoic acid (11) based on mass and various nuclear magnetic resonance (NMR) spectroscopic techniques. This is the first isolation of epicatechin and catechin from the genus Amomum. The radical scavenging activity of the isolated compounds was evaluated using 1,1-diphenyl-2-picryIhydrazyl (DPPH) and colorimetric and electron spin resonance (ESR) analyses. The antioxidant activity of the compounds was also determined based on the oxidative stability index (OSI). The catechins and catechol derivatives showed strong activities in both the DPPH radical scavenging activity and antioxidant activity assays., AMER OIL CHEMISTS SOC A O C S PRESS, Jun. 2000, JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 77 (6), 667 - 673, web_of_science

    Scientific journal

  • Antibacterial Compounds from Nutmeg against Upper Airway Respiratory Tract Bacteria

    KIKUZAKI Hiroe; Yasuo Tanaka; Seiji Fukuda; Hiroe Kikuzaki; Nobuji Nakatani

    May 2000, ITE Letters on Batteries, New Technologies & Medicine,, 1, 412-417

  • Isolation and structural elucidation of two new glycosides from sage (Salvia officinalis L.)

    MF Wang; H Kikuzaki; NQ Zhu; SM Sang; N Nakatani; CT Ho

    Six compounds, 1-O-(2,3,4-trihydroxy-3-methyl)butyl-6-O-feruloyl-beta-D-glucopyranoside, ethyl beta-D-glucopyranosyl tuberonate, p-hydroxybenzoic acid, (-)-hydroxyjasmonic acid, caffeic acid, and 4-hydroxyacetophenone 4-O-[5-O-(3,5-dimethoxy-4-hydroxybenzoyl)-beta-D-apiofrunosyl]-(1-->2)-beta-D-glucopyranoside, were isolated from the n-butanol-soluble fraction of sage leaf extracts. Their structures were determined by spectral methods (MS, NMR, and BD-NMR), and their antioxidant activities were measured. Among them, two new glycosides were elucidated. All of these compounds showed DPPH free radical scavenging activity at the concentration of 30 mM, and caffeic acid was the most active compound., AMER CHEMICAL SOC, Feb. 2000, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 48 (2), 235 - 238, web_of_science

    Scientific journal

  • Screening of Zingiberaceae Extracts for Antimicrobial and Antioxidant Activities

    KIKUZAKI Hiroe; M. Habsah; M. Amran; M.M. Mackeen; N.H. Lajis; H. Kikuzaki; N. Nakatani; A.A. Rahman, Ghafar; A.M. Ali

    Feb. 2000, Journal of Ethnopharmacology, 72, 403-410

  • Galloylglucosides from Berries of Pimenta dioica

    KIKUZAKI Hiroe; Hiroe Kikuzaki; Akemi Sato; Yoko Mayahara; Nobuji Nakatani

    Jan. 2000, J. Nat. Prod, 63, 749-752

  • Antioxidative phenylpropanoids from berries of Pimenta dioica

    H Kikuzaki; S Hara; Y Kawai; N Nakatani

    A phenylpropanoid, threo-3-chloro-1-(4-hydroxy-3-methoxy phenyl)propane-1,2-diol, was isolated from the berries of Pimenta dioica together with five known compounds. eugenol, 4-hydroxy-3-methoxycinnamaldehyde, 3,4-dimethoxycinnamaldehyde, vanillin and 3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol. In addition, the stereochemistry of 3-(4-hydroxy-3-methoxyphenyl)propane- 1,2-diol was determined. The phenylpropanoids inhibited autoxidation of linoleic acid in a water-alcohol system. (C) 1999 Elsevier Science Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD, Dec. 1999, PHYTOCHEMISTRY, 52 (7), 1307 - 1312, web_of_science

    Scientific journal

  • Two C-21-steroidal glycosides isolated from Cynanchum stauntoi

    NQ Zhu; MF Wang; H Kikuzaki; N Nakatani; CT Ho

    Studies on the roots of Cynanchum stauntoi led to the isolation of two C-21-steroidal glycosides, formally named stauntosides A and B. Their structures were elucidated on the basis of spectroscopic evidence, especially that from analysis of 2D-NMR spectra. They were found to possess an unusual skeleton and were identified as stauntogenin 3-O-alpha-L-diginopyranosyl-(1-4)beta-L-cymaropyranosyl-(1-4)-beta-D-digitoxopyranosyl-(1-4)-beta-D-thevetopyranoside and stauntogenin 3-O-alpha-L-cymaropyranosyl-(1-4)-beta-D-digitoxopyranosyl-(1-4)-beta-D-3-demethyl-2-deoxy-thevetopyranoside (C) 1999 Elsevier Science Ltd. All rights reserved., PERGAMON-ELSEVIER SCIENCE LTD, Dec. 1999, PHYTOCHEMISTRY, 52 (7), 1351 - 1355, web_of_science

    Scientific journal

  • Novel trisaccharide fatty acid ester identified from the fruits of Morinda citrifolia (Noni)

    MF Wang; H Kikuzaki; K Csiszar; CD Boyd; A Maunakea; SFT Fong; G Ghai; RT Rosen; N Nakatani; CT Ho

    Two known glycosides and a novel trisaccharide fatty acid ester were isolated from the n-butanol-soluble fraction of the fruits of Morinda citrifolia (noni). Structure determination was carried out by spectral techniques such as MS, IR, NMR, and BD-NMR. The novel trisaccharide fatty acid ester was elucidated as 2,6-di-O-(beta-D-glucopyranosyl)-1-O-octanoyl-beta-D-glucopyranose. The known compounds were identified as rutin and asperulosidic acid., AMER CHEMICAL SOC, Dec. 1999, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 47 (12), 4880 - 4882, web_of_science

    Scientific journal

  • 二倍体および四倍体ショウガの辛味成分と官能検査による辛味性評価ならびにメタノール抽出物の抗酸化性

    KIKUZAKI Hiroe

    Jun. 1999, 熱帯農業, 43, 71-75

  • Antioxidant effect of the constituents of Susabinori (Porphyra yezoensis)

    R Nakayama; Y Tamura; H Kikuzaki; N Nakatani

    The antioxidant activities of several extracts from Susabinori (Porphyra yezoensis) were measured by the ferric thiocyanate method and the thiobarbituric acid method. The methanol, acetone, ethyl acetate, and hexane extracts, and the chloroform-soluble and water-soluble fractions from the chloroform-methanol extract exhibited higher activities than a-tocopherol. The hot water extract showed little activity. Thin-layer chromatography analysis of the active extracts suggested the existence of several antioxidants. The activity of the chloroform soluble fraction was due to chlorophyll analogs. A strong antioxidant was isolated from the methanol extract, accompanied by several amino acids such as leucine and phenylalanine. This compound was identified as usujilene, a kind of mycosporine-glycine like amino acid., AMER OIL CHEMISTS SOC A O C S PRESS, May 1999, JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 76 (5), 649 - 653, web_of_science

    Scientific journal

  • Acetophenone glycosides from thyme (Thymus vulgaris L.)

    MF Wang; H Kikuzaki; CC Lin; A Kahyaoglu; MT Huang; N Nakatani; CT Ho

    Four acetophenone glycosides were isolated from the butanol-soluble fraction of thyme extracts. Their structures were determined by spectral methods (MS, NMR, and 2D-NMR). Among them, two new compounds, 4-hydroxyacetophenone 4-O-[5-O-(3,5-dimethoxy-4-hydroxybenzoyl)-beta-D-apiofuranosyl]-(1-->2)-beta-D-glucopyranoside (1) and 4-hydroxyacetophenone 4-O-[5-O-(4-hydroxybenzoyl)-beta-D-apiofuranosyl]-(1-->2)-beta-D-glucopyranoside (2), were determined. Compound 1 showed weak cytotoxicity, inhibiting DNA synthesis of human leukemia cells., AMER CHEMICAL SOC, May 1999, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 47 (5), 1911 - 1914, web_of_science

    Scientific journal

  • New bitter diterpenes, rabdosianone I and II, isolated from Isodon japonicus Hara

    Y Yamada; N Sako; E Ando; M Yamada; H Kikuzaki; T Yamamoto

    The new bitter diterpenes, rabdosianone I(C20H24O5) and II (C22H28O6), were isolated from Isodon japonicus (Japanese name, enmeiso), and their structures were elucidated by spectroscopic methods, Electrophysiological experiments were performed to compare rabdosianone I with quinine. The taste responses of chorda tympani nerves to rabdosianone I were smaller than those to quinine in Wistar rats., TAYLOR & FRANCIS LTD, Mar. 1999, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 63 (3), 524 - 529, web_of_science

    Scientific journal

  • Spasmolytic activity of aurapten analogs

    Y Yamada; M Okamoto; H Kikuzaki; N Nakatani

    Seven coumaric compounds analogous to aurapten were synthesized. Their spasmolytic activity against Ba2+, acetylcholine and histamine was evaluated to investigate their structure-activity relationship. The results of the bioassay demonstrated the important roles of the cis type of double bond at C-2' and the epoxide between C-6' and 7'., TAYLOR & FRANCIS LTD, Apr. 1997, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 61 (4), 740 - 742, web_of_science

    Scientific journal

  • Antioxidative Activity of Constituents from Fennel Seeds

    KIKUZAKI Hiroe; Reiko Nakayama; Hiroe Kikuzaki; Nobuji Nakatani; Hisashi Horiuchi

    Dec. 1996, J. Home Econ. Jpn., 47, 1193-1199

  • 延命草(Isodon japonicus) に含まれる新規な苦味化合物の構造

    KIKUZAKI Hiroe

    Dec. 1996, 日本味と匂学会誌, 3, 584-587

  • Stability of anthocyanins of Sambucus canadensis and Sambucus nigra

    O Inami; Tamura, I; H Kikuzaki; N Nakatani

    Elderberry anthocyanins (Sambucus nigra) including acylated derivatives (Sambucus canadensis) were studied for use as beverage pigments. Cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside-5-O-beta-D-glucopyranoside from S. canadensis was more stable than cyanidin 3-sambubioside from S. nigra. Acylation improved both heat and light stability, whereas glycosidation only stabilized anthocyanins in the presence of light. Cyanidin 3-(E)-p-coumaroyl-sambubioside-5-glucoside changed to three new anthocyanins under light irradiation. These three anthocyanins were isolated, and their structures were identified to be cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside-5-O-beta-D-glucopyranoside, cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside, and cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-beta-D-xylopyranosyl)-beta-D-glucopyranoside., AMER CHEMICAL SOC, Oct. 1996, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 44 (10), 3090 - 3096, web_of_science

    Scientific journal

  • Cyclic diarylheptanoids from rhizomes of Zingiber officinale

    H Kikuzaki; N Nakatani

    Five new diarylheptanoids were isolated from the rhizomes of Zingiber officinale and their structures elucidated by spectroscopic and chemical methods. They were oxygenated at C-1, 3 and 5 on the heptane chain and cyclized between C-1 and C-5 through oxygen. Copyright (C) Elsevier Science Ltd, PERGAMON-ELSEVIER SCIENCE LTD, Sep. 1996, PHYTOCHEMISTRY, 43 (1), 273 - 277, web_of_science

    Scientific journal

  • Secoiridoid glucosides from Swertia mileensis

    Hiroe Kikuzaki; Yoko Kawasaki; Seiichi Kitamura; Nobuji Nakatani

    Five new acyl secoiridoid glucosides along with three known secoiridoid glucosides, swertiamarin, 2'-O-acetylswertiamarin, and amarogentin, were isolated from the aerial parts of Swertia mileensis. The structures of these compounds were determined to be 2'-O-acetyl-4'-O-trans-feruloylswertiamarin, 2'-O-acetyl-4'-O-cis-feruloylswertiamarin, 2'-O-acetyl-4'-O-trans-p-coumaroylswertiamarin, 2'-O-acetyl-4'-O-cis-p-coumaroylswertiamarin, and 4'-O-trans-p-coumaroylswertiamarin from spectroscopic evidence., Georg Thieme Verlag, 1996, Planta Medica, 62 (1), 35 - 38, doi;pubmed

    Scientific journal

  • Acylated anthocyanins from fruits of Sambucus canadensis

    Nobuji Nakatani; Hiroe Kikuzaki; Junko Hikida; Mayumi Ohba; Osamu Inami; Itaru Tamura

    Two new acylated anthocyanin glycosides were isolated from fruits of Sambucus canadensis. The structures were determined to be cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside)-5-O-β-d-glucopyranoside and cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside. © 1995., 1995, Phytochemistry, 38 (3), 755 - 757, doi;pubmed

    Scientific journal

  • LABDANE DITERPENES FROM RHIZOMES OF HEDYCHIUM-CORONARIUM

    N NAKATANI; H KIKUZAKI; H YAMAJI; K YOSHIO; C KITORA; K OKADA; WG PADOLINA

    Three new labdane diterpenes were isolated together with four known diterpenes [(E)-labda-8(17),12-diene-15,16-dial, coronarin B and D, and isocoronarin D] from rhizomes of Hedychium coronarium. The structures of the new compounds were determined by spectroscopic evidence to be labda-8(17),11,13-trien-15(16)-olide, an ester of labda-8(17), 11,13-trien-15-al-16-oic acid and isocoronarin D, and 7 beta-hydroxycoronarin B., PERGAMON-ELSEVIER SCIENCE LTD, Nov. 1994, PHYTOCHEMISTRY, 37 (5), 1383 - 1388, web_of_science

    Scientific journal

  • TRANSFORMATION OF GLYCYRRHIZIC ACID BY ASPERGILLUS SPP

    Y YAMADA; A NAKAMURA; K YAMAMOTO; H KIKUZAKI

    Glycryrrhizic acid was metabolized to 3-oxo-18 beta-glycyrrhetinic acid via 18 beta-glycyrrhetinic acid by Aspergillus niger, A. oryzae, A. sojae, and A. tamarii. Two methyl esters were derived from these two metabolites and identified by their C-13-NMR spectra and MS data., TAYLOR & FRANCIS LTD, Feb. 1994, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 58 (2), 436 - 437, web_of_science

  • STRUCTURE OF ANTIOXIDATIVE COMPOUNDS IN GINGER

    H KIKUZAKI; Y KAWASAKI; N NAKATANI

    Fourteen gingerol related compounds and seven diarylheptanoids were isolated from ginger rhizomes. Their structures were elucidated on the basis of chemical and spectroscopic evidence. Most of the isolated compounds exhibited stronger antioxidative effect than alpha-tocopherol. The antioxidant activity depended upon substituents on the side chain and benzene ring, and the length of side chain., AMER CHEMICAL SOC, 1994, FOOD PHYTOCHEMICALS FOR CANCER PREVENTION II, 547, 237 - 243, web_of_science

    Scientific journal

  • LC-MS ANALYSIS AND STRUCTURAL DETERMINATION OF NEW AMIDES FROM JAVANESE LONG PEPPER (PIPER-RETROFRACTUM)

    H KIKUZAKI; M KAWABATA; E ISHIDA; Y AKAZAWA; Y TAKEI; N NAKATANI

    Amides in a CH2Cl2 extract from the fruits of Piper retrofractum were detected by HPLC/APCI-MS. Seven new unsaturated amides, together with six known ones, were isolated, and their structures were determined to be N-isobutyl-2E,4E,12Z-octadecatrienamide (1), N-isobutyl-2E,4E,14Z-eicosatrienamide (2), 1-(octadeca-2E,4E,12Z-trienoyl)piperidine (3), 1-(eicosa-2E,4E,14Z-trienoyl)piperidine (4), 1-(octadeca-2E,4E-dienoyl)piperidine (5), 1-(eicosa-2E,4E-dienoyl)piperidine (6), and 1-(eicosa-2E,14Z-dienoyl)piperidine (7) on the basis of chemical and spectroscopic evidence., TAYLOR & FRANCIS LTD, Aug. 1993, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 57 (8), 1329 - 1333, web_of_science

    Scientific journal

  • Antioxidant Effects of Some Ginger Constituents

    HIROE KIKUZAKI; NOBUJI NAKATANI

    The nonvolatile fraction of the dichloromethane extract of ginger rhizomes exhibited a strong antioxidative activity using linoleic acid as the substrate in ethanol‐phosphate buffer solution. The fraction was purified by chromatographic techniques to provide five gingerol related compounds and eight diarylheptanoids. Among them, 12 compounds exhibited higher activity than α‐tocopherol. The activity was probably dependent upon side chain structures and substitution patterns on the benzene ring. Copyright © 1993, Wiley Blackwell. All rights reserved, 1993, Journal of Food Science, 58 (6), 1407 - 1410, doi

    Scientific journal

  • 2 CURCUMINOID PIGMENTS FROM CURCUMA-DOMESTICA

    R NAKAYAMA; Y TAMURA; H YAMANAKA; H KIKUZAKI; N NAKATANI

    Two new pigments were isolated from fresh rhizomes of turmeric (Curcuma domestica Val.). Their structures were determined to be 1-(4-hydroxy-3-methoxyphenyl)-7-(3,4-dihydroxyphenyl)-1,6-heptadiene-3,5-dione and 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one by spectroscopic methods., PERGAMON-ELSEVIER SCIENCE LTD, May 1993, PHYTOCHEMISTRY, 33 (2), 501 - 502, web_of_science

  • Identification of Antimicrobial Gingerols from Ginger (Zingiber officinale Roscoe)

    KIKUZAKI Hiroe; Yasumasa Yamada; Hiroe Kikuzaki; Nobuji Nakatani

    Jun. 1992, J. Antibact. Antifung. Agents, 20, 309-311

  • CHARACTERIZATION OF HIGH-AMYLOSE TYPE ENDOSPERM STARCHES OF RICE PLANTS CULTIVATED IN ASIA

    H FUWA; K OKUNO; R ASASHIBA; H KIKUZAKI; M ASAOKA; N INOUCHI; Y SUGIMOTO

    Starch granules were prepared from 14 indica rice grains which were collected from various places of Asia and had the amylose content of 28% and over by a blue value method. The starches were high- and medium-amylose types by the enzymatic and chromatographic method. However, none of them belonged to the type of high amylose mutants which were induced by the chemical mutagen treatment of a japonica rice cultivar: Kinmaze and possessed similar characteristics of endosperm starch to that of the amylose-extender (ae) mutant of maize (Zea mays L.)., VCH PUBLISHERS INC, Jun. 1992, STARCH-STARKE, 44 (6), 203 - 205, web_of_science

    Scientific journal

  • CONSTITUENTS OF ZINGIBERACEAE .5. GINGERDIOL RELATED-COMPOUNDS FROM THE RHIZOMES OF ZINGIBER-OFFICINALE

    H KIKUZAKI; SM TSAI; N NAKATANI

    [6]-Gingerdiol and four analogues, (3R,5S)-5-acetoxy-3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decane, (3R,5S)-3-acetoxy-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decane,(3R,5S)-3,5-diacetoxy-1-(4-hydroxy-3-methoxyphenyl)decane and (3R,5S)-3,5-diacetoxy-1-(3,4-dimethoxyphenyl)decane, were isolated from the rhizomes of Zingiber officinale. Their structures were established by chemical and spectroscopic evidence., PERGAMON-ELSEVIER SCIENCE LTD, May 1992, PHYTOCHEMISTRY, 31 (5), 1783 - 1786, web_of_science

    Scientific journal

  • New Phenolic Compounds of Ginger (Zingiber officinale Roscoe)

    KIKUZAKI Hiroe; Nobuji Nakatani; Hiroe Kikuzaki

    Mar. 1992, Chemistry Express, 7, 221-224

  • MICROBIAL TRANSFORMATION OF (+/-)-EPOXYAURAPTEN BY PSEUDOMONAS-AERUGINOSA

    Y YAMADA; H KIKUZAKI; N NAKATANI

    TAYLOR & FRANCIS LTD, Jan. 1992, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 56 (1), 153 - 154, web_of_science

  • Constituents of Gyrophora esculenta

    KIKUZAKI Hiroe; Nobuji Nakatani; Chon Ho Kim; Hiroe Kikuzaki; Teruyo Muroi; Yoko Matsumura

    Aug. 1991, Chemistry Express, 6, 587-590

  • CONSTITUENTS OF ZINGIBERACEAE .1. DIARYLHEPTANOIDS FROM THE RHIZOMES OF GINGER (ZINGIBER-OFFICINALE ROSCOE)

    H KIKUZAKI; J USUGUCHI; N NAKATANI

    Two new diarylheptanoids, meso-3,5-diacetoxy-1,7-bis(4-hydroxy-3-methoxyphenyl)heptane (3a) and 3,5-diacetoxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)heptane (4a) were isolated from the rhizomes of ginger (Zingiber officinale ROSCOE), together with dehydroxytetrahydrocurcumin (gingerenone A, 1) and hexahydrocurcumin (2). Their structures were elucidated on the basis of chemical and spectroscopic evidence., PHARMACEUTICAL SOC JAPAN, Jan. 1991, CHEMICAL & PHARMACEUTICAL BULLETIN, 39 (1), 120 - 122, web_of_science

    Scientific journal

  • CONSTITUENTS OF PIPERACEAE .4. ANTIMICROBIAL PHENYLPROPANOIDS FROM PIPER-SARMENTOSUM

    T MASUDA; A INAZUMI; Y YAMADA; WG PADOLINA; H KIKUZAKI; N NAKATANI

    One new and three known phenylpropanoids have been isolated from the leaves of Piper sarmentosum. They are 1-allyl-2,6-dimethoxy-3,4-methylenedioxybenzene, 1-allyl-2,4,5-trimethoxybenzene, 1-(1-E-propenyl)-2,4,5-trimethoxybenzene and 1-allyl-2-methoxy-4,5-methylenedioxybenzene. The new compound showed antimicrobial activity against Escherichia coli and Bacillus subtilis., PERGAMON-ELSEVIER SCIENCE LTD, 1991, PHYTOCHEMISTRY, 30 (10), 3227 - 3228, web_of_science

    Scientific journal

  • CONSTITUENTS OF ZINGIBERACEAE .4. DIARYLHEPTANOIDS FROM RHIZOMES OF ZINGIBER-OFFICINALE

    H KIKUZAKI; M KOBAYASHI; N NAKATANI

    Six new diarylheptanoids, 5-hydroxy-7-(4-hydroxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-3-heptanone, 3,5-diacetoxy-7-(3,4-dihydroxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)heptane, 5-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-3-heptanone, 5-hydroxy-7-(4-hydroxy-3,5-dimethoxyphenyl)-1-(4-hydroxy-3-methoxyphenyl)-3-heptanone, (3R,5S)-3,5-dihydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)heptane and (3S,5S)-3,5-diacetoxy-1,7-bis(3,4-dihydroxyphenyl)heptane were isolated from the rhizomes of Zingiber officinale, together with two known diarylheptanoids. Their structures were established by chemical and spectroscopic evidences., PERGAMON-ELSEVIER SCIENCE LTD, 1991, PHYTOCHEMISTRY, 30 (11), 3647 - 3651, web_of_science

    Scientific journal

  • Synthesis of Capsaicinol [1]

    KIKUZAKI Hiroe; Toshiya Masuda; Hiroe Kikuzaki; Yukari Tachibana; Nobuji Nakatani

    Jun. 1990, Chemistry Express, 5, 369-372

  • STRUCTURE OF A NEW ANTIOXIDATIVE PHENOLIC-ACID FROM OREGANO (ORIGANUM-VULGARE L)

    H KIKUZAKI; N NAKATANI

    JAPAN SOC BIOSCI BIOTECHN AGROCHEM, Feb. 1989, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 53 (2), 519 - 524, web_of_science

    Scientific journal

  • Antioxidative Compounds from Edible Plants: Phenolic Amides from Capsicum frutescens L.

    KIKUZAKI Hiroe; Nobuji Nakatani; Yukari Tachibana; Hiroe Kikuzaki

    1989, Medical, Biochemical and Chemical Aspects of Free Radicals, 453-456

  • LIGNANS OF PAPUAN MACE (MYRISTICA-ARGENTEA WARB) .2. DIARYLDIMETHYLBUTANE LIGNANS FROM MYRISTICA-ARGENTEA AND THEIR ANTIMICROBIAL ACTION AGAINST STREPTOCOCCUS MUTANS

    N NAKATANI; K IKEDA; H KIKUZAKI; M KIDO; Y YAMAGUCHI

    PERGAMON-ELSEVIER SCIENCE LTD, 1988, PHYTOCHEMISTRY, 27 (10), 3127 - 3129, web_of_science

    Scientific journal

  • A NEW ANTIOXIDATIVE GLUCOSIDE ISOLATED FROM OREGANO (ORIGANUM-VULGARE L)

    N NAKATANI; H KIKUZAKI

    JAPAN SOC BIOSCI BIOTECHN AGROCHEM, Oct. 1987, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 51 (10), 2727 - 2732, web_of_science

    Scientific journal

  • Structure of Diacylated Anthocyanins from Red Cabbage (Brassica oleracea)

    KIKUZAKI Hiroe; Nobuji Nakatani; Kayo Ikeda; Mikio Nakamura; Hiroe Kikuzaki

    Sep. 1987, Chemistry Express, 2, 555-558

  • Structure of Two Acylated Anthocyanins from Red Cabbage (Brassica oleracea)

    KIKUZAKI Hiroe; Kayo Ikeda; Hiroe Kikuzaki; Mikio Nakamura; Nobuji Nakatani

    Sep. 1987, Chemistry Express, 2, 563-366

  • 大豆油の消化管内移動におよぼすペクチンの影響

    KIKUZAKI Hiroe

    Jan. 1987, 日本栄養・食糧学会誌, 40, 64-69

  • Isolation of phenolic components from strawberry cultivar 'Tokun' and their inhibitory activities on recombinant human histidine decarboxylase

    Yoko Nitta; Miyuki Mori; Yuji Noguchi; Yuichi Uno; Misaki Ishibashi; Hiroshi Ueno; Hiroe Kikuzaki

    Aug. 2020, J. Biol. Macromol., 20 (2), 33 - 39

    Scientific journal

  • The ellagitannin trimer rugosin G inhibits recombinant human histidine decarboxylase.

    Yoko Nitta; Hideyuki Ito; Hirohumi Komori; Hiroshi Ueno; Daiki Takeshima; Mikiko Ito; Motoyoshi Sakaue; Hiroe Kikuzaki

    Rugosin G, an ellagitannin trimer, was isolated from the water-soluble fraction of red rose petals, and its inhibitory activity against recombinant human histidine decarboxylase was investigated. Rugosin G showed potent inhibition compared to ellagitannin monomers and a dimer with macrocyclic structure (oenothein B), suggesting the potent inhibition of rugosin G was attributed to its linear oligomeric conformation. Abbreviations: HDC, histidine decarboxylase; Me2CO, acetone; EtOAc, ethyl acetate., Jul. 2019, Bioscience, biotechnology, and biochemistry, 83 (7), 1315 - 1318, True, doi;pubmed

    Scientific journal

  • Components for Inhibiting Lipid Oxidation Related to Discoloration of Carotenoid Contained in Sugarcane Extract

    Hiroe Kikuzaki; Mihoko Masuda; Eri Kishi; Mina Ozaki; Kanako Kondo; Aya Kanai; Kazuyo Shiomi; Toma Furuta; Masami Mizu; Yukie Nagai

    Japanese Society for Food Science and Technology, 2019, Food Science and Technology Research, 25 (5), 715 - 725, doi;url

    Scientific journal

  • Biological Activities of Isolated Compounds from Three Edible Malaysian Red Seaweeds, Gracilaria changii, G. manilaensis and Gracilaria sp.

    Yosie Andriani; Desy Fitrya Syamsumir; Tee Ching Yee; Faizah Shaharom Harisson; Gan Ming Herng; Siti Aishah Abdullah Christine Abellana Oroscoa; Christine Abellana Orosco; Abdul Manaf Ali; Jalifah Latip; Hiroe Kikuzaki; Habsah Mohamad

    Gracilaria species are red marine macroalgae that are found abundantly in Malaysia. Gracilaria changii from Morib, Selangor, G. manilaensis and Gracilaria sp. from Gelang Patah, Johor were used in this study. Five compounds were successfully isolated and identified as hexadecanoic acid (1), cholest-5-en-3-ol (2), 2-hydroxymyristic acid (3), cholesteryl myristate (4) and 1-(4'-methoxyphenyl)-3-(2 '',4 '',6 ''-trihydroxyphenyl)-3-hydroxypropanone (5) based on spectral data analysis (IR, UV, GC-MS, H-1 NMR, C-13 NMR, HMQC and HMBC). All compounds isolated were tested for cytotoxicity (MTT assay for HL-60 and MCF-7 cell lines), and antibacterial (disc diffusion method), antioxidant (DPPH free radical scavenging assay and xanthine oxidase inhibitory assay) and acetylcholinesterase inhibitory (AChE) activity (TLC bioautographic method). Compounds 1 and 3 exhibited strong cytotoxic activity against HL-60 and MCF-7 cell lines. Compound 5 showed high antioxidant activity in both the DPPH free radical scavenging and xanthine oxidase inhibition assays. Compound 1 showed positive activity for AChE inhibitory with a minimum inhibition dose of 0.625 mu g sample. All compounds demonstrated antibacterial activity producing 8 to 14 mm inhibition zones. A positive control was applied to all bioassays and experiments were performed with three replicates. Results demonstrated that three edible red seaweeds are rich sources of bioactive compounds with potential application for pharmaceutical purposes., NATURAL PRODUCTS INC, Aug. 2016, NATURAL PRODUCT COMMUNICATIONS, 11 (8), 1117 - 1120, web_of_science

    Scientific journal

  • Antioxidants from some tropical spices

    H Kikuzaki

    AMER CHEMICAL SOC, 2003, ORIENTAL FOODS AND HERBS, 859, 176 - 189, web_of_science

    Scientific journal

MISC

  • フトモモ科スパイス(クローブ,オールスパイス)の食文化と機能性

    KIKUZAKI Hiroe

    Dec. 2017, Functional Food, 11 (2), 111-116

  • ショウガの食文化と機能性

    KIKUZAKI Hiroe

    Dec. 2017, Functional Food, 11 (2), 122-128

  • ショウガ科香辛料の特徴的成分~その機能性~

    KIKUZAKI Hiroe

    Aug. 2017, AROMA RESEARCH, 18 (3), 42-47

  • ガロタンニン,エラジタンニンによるヒスチジン脱炭酸酵素の活性阻害の解析

    森美幸; 菊崎泰枝; 植野洋志; 小森博文; 新田陽子

    25 Apr. 2016, ビタミン, 90 (4), 184, j_global;url

  • ローズペタル成分によるヒト由来ヒスチジン脱炭酸酵素の阻害

    森美幸; 渡邉裕香; 新田陽子; 植野洋志; 秋本梢; 小林慧子; 菊崎泰枝

    01 Sep. 2015, 生物高分子, 15 (1), 74, j_global;url

  • メドウスイート抽出液によるサバのヒスタミン汚染抑制

    安方芙実子; 北元憲利; 新田陽子; 江戸良也子; 植野洋志; 菊崎泰枝

    01 Sep. 2015, 生物高分子, 15 (1), 74, j_global;url

  • Rosa gallicaの花弁に含まれるヒスチジン脱炭酸酵素阻害成分の探索

    秋本梢; 渡邉裕香; 新田陽子; 植野洋志; 小林慧子; 菊崎泰枝

    05 Mar. 2014, 日本農芸化学会大会講演要旨集(Web), 2014, 4B04A08 (WEB ONLY), j_global;url

  • ヒスタミン合成を阻害する薬用植物成分の探索

    胡榴; 新田陽子; 菊崎泰枝; 植野洋志

    01 Oct. 2008, 生物高分子, 8 (3), 87, j_global;url

  • Antimicrobial Activity of Saponins from Leaves of Bacopa monnieri

    Masashi Hisamoto; Naomi Nishihara; Tohru Okuda,Huong Lai Mai; Hiroe Kikuzaki; Koki Yokotsuka; Koki Yokotsuka

    2008, Journal of Clinical Biochemistry and Nutrition, 43, 1 - 3

  • ヒト由来ヒスチジンデカルボキシラーゼの活性を阻害する香辛料成分

    新田陽子; 菊崎泰枝; 植野洋志

    2008, 日本農芸化学会関西支部講演会講演要旨集, 455th, 16, j_global;url

  • ショウガ科植物の化学成分

    KIKUZAKI Hiroe

    Mar. 2007, 香料, 233, 81-87

  • ヒスチジン脱炭酸酵素の活性を阻害する食品成分

    新田陽子; 菊崎泰枝; 植野洋志

    01 Jul. 2006, 生物高分子, 6 (2), 57, j_global;url

  • オールスパイス(ピメント)の抗酸化成分の解明

    KIKUZAKI Hiroe

    Oct. 2002, 香料, 215, 101-106

  • 沖縄の伝統的食用植物の機能性

    KIKUZAKI Hiroe

    Jul. 2001, FOOD Style 21, 5 (7), 50-53

  • Antioxidants in the ginger family.

    N Nakatani; H Kikuzaki

    AMER CHEMICAL SOC, Aug. 2000, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 220, U42 - U42, web_of_science

    Summary international conference

  • ANTIOXIDANT ACTIVITY OF POLYPHENOLS IN FOOD

    N NAKATANI; H KIKUZAKI

    JAPAN SOC BIOSCI BIOTECHN AGROCHEM, Sep. 1995, NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 69 (9), 1189 - 1192, web_of_science

    Book review

  • 食品中のポリフェノールの抗酸化活性

    KIKUZAKI Hiroe

    Sep. 1995, 日本農芸化学会誌, 69 (9), 1189-1192

  • 香辛植物の抗酸化物質とその生体内酸化障害抑制作用

    KIKUZAKI Hiroe

    Mar. 1995, フードケミカル, 3, 49-53

  • スパイス・ハーブの化学と機能

    KIKUZAKI Hiroe

    Oct. 1994, 食品工業, 37 (19), 57-65

  • ハーブおよびその精油の抗菌作用と食品への応用

    KIKUZAKI Hiroe

    Sep. 1994, aromatopia, 9 (3), 42-46

  • ローズマリーの抗菌成分とその活性

    KIKUZAKI Hiroe

    Nov. 1993, aromatopia, 3 (4), 40-42

  • STRUCTURE OF ANTIOXIDATIVE COMPOUNDS OF GINGER

    H KIKUZAKI; Y KAWASAKI; N NAKATANI

    AMER CHEMICAL SOC, Aug. 1992, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 204, 139 - AGFD, web_of_science

    Summary international conference

  • 香辛料成分の構造特性と食品劣化抑制機能

    KIKUZAKI Hiroe

    Mar. 1992, 食品と開発, 27 (3), 7-10

  • Structure of Iridoids from Swertia mileensis He et Shi

    KIKUZAKI Hiroe; Hiroe Kikuzaki; Seiichi Kitamura; Nobuji Nakatani

    Dec. 1988, Chemistry Express, 3, 751-754

  • ナツメグ、メースの抗菌・抗酸化作用

    KIKUZAKI Hiroe

    Feb. 1988, フードケミカル, 2, 70-74

  • Recent Studies on the Application of Data for Food Components Based on Mass Spectrometry

    菊﨑泰枝

    Oct. 2020, Food & Food Ingredients Journal of Japan, 225 (4), 339 - 345

    Introduction other

  • 薬用植物を使用したアレルギー様食中毒予防の研究

    新田陽子; 菊﨑泰枝

    30 Dec. 2020, アレルギーの臨床, 40 (14), 50 - 52

    Introduction scientific journal

Books etc

  • 新スタンダード栄養・食物シリーズ5 食品学-食品成分と機能性-

    KIKUZAKI Hiroe (, Range: 分担)

    東京化学同人, Apr. 2016, 79-98, 107-108, 253-262 (ISBN: 9784807916658)

  • 栄養科学イラストレイテッド 食品学II 食品の分類と特性、加工を学ぶ

    KIKUZAKI Hiroe (, Range: 分担)

    羊土社, Mar. 2016, 148-154 (ISBN: 9784758108805)

  • 管理栄養士のための経営管理 アメリカにおける考え方と実践を学ぶ

    KIKUZAKI Hiroe (, Range: 分担)

    東京教学社, Dec. 2015, 522-545 (ISBN: 9784808260439)

  • 栄養科学イラストレイテッド 食品学I 食品の成分と機能を学ぶ

    KIKUZAKI Hiroe (, Range: 編集)

    羊土社, Nov. 2015, 90-91, 100-122 (ISBN: 9784758108799)

  • 新訂 食健康科学

    KIKUZAKI Hiroe (, Range: 分担)

    放送大学教育振興会, Mar. 2015, 27-62, 102-122 (ISBN: 9784595140471)

  • Development & Market of Antioxidant

    KIKUZAKI Hiroe (, Range: 分担)

    シーエムシー出版, May 2014, 47-62

  • 生食のおいしさとリスク

    KIKUZAKI Hiroe (, Range: 分担)

    エヌ・ティー・エス, Jun. 2013, 459-466 (ISBN: 9784864690669)

  • 改訂版 食と健康

    KIKUZAKI Hiroe (, Range: 分担)

    放送大学教育振興会, Mar. 2012, 28-57, 103-119 (ISBN: 9784595313493)

  • スパイスなんでも小事典-おいしくて体によい使い方

    KIKUZAKI Hiroe (, Range: 分担)

    講談社ブルーバックス, Sep. 2011, 57-59, 81-83, 164-168 (ISBN: 9784062576987)

  • 栄養科学ファウンデーションシリーズ 給食経営管理論

    KIKUZAKI Hiroe (, Range: 分担)

    朝倉書店, Mar. 2011, 1-4

  • 新版 食べ物と健康[食品学総論]

    KIKUZAKI Hiroe (, Range: 分担)

    八千代出版, Feb. 2011, 7-30, 55-75

  • 新しい食品加工学

    KIKUZAKI Hiroe (, Range: 分担)

    南江堂, Jan. 2011, 135-137

  • Spices and Herbs-Food Functions and Application Technics

    KIKUZAKI Hiroe (, Range: 編集)

    シーエムシー出版, Jan. 2011, 42-44、50-56、85-92、127-135

  • 健康と栄養のための有機化学

    KIKUZAKI Hiroe (, Range: 分担)

    建帛社, Apr. 2010, 138-165

  • PDCAに基づく給食経営管理実習

    KIKUZAKI Hiroe (, Range: 分担)

    化学同人, Apr. 2009, 13-14, 31-36

  • 食健康科学

    KIKUZAKI Hiroe (, Range: 分担)

    放送大学教育振興会, Mar. 2009, 101-148,254-265

  • スタンダード栄養・食物シリーズ5 食品学-食品成分と機能性 第2版

    KIKUZAKI Hiroe (, Range: 分担)

    東京化学同人, Sep. 2008, 76-96, 104-105, 257-265

  • 食品機能性の科学

    KIKUZAKI Hiroe (, Range: 分担)

    Apr. 2008, 55-59、1042-1045

  • 栄養・食糧学用語辞典

    KIKUZAKI Hiroe (, Range: 分担)

    建帛社, Oct. 2007

  • 管理栄養士コースで学ぶ?キャリアデザインのために?

    KIKUZAKI Hiroe (, Range: 分担)

    同文書院, Apr. 2007, 60-62

  • 食品学実験

    KIKUZAKI Hiroe (, Range: 編集)

    光生館, Apr. 2007, 87-113, 148-153

  • 基礎調理実習 食品・栄養・大量調理へのアプローチ

    KIKUZAKI Hiroe (, Range: 分担)

    化学同人, Mar. 2007, 65-83

  • 食品と技術・「大学の研究室-大阪市立大学大学院生活科学研究科食・健康科学講座菊﨑研究室-」

    KIKUZAKI Hiroe (, Range: 筆頭著者)

    食品産業センター, Jan. 2007, 10-13

  • 2007管理栄養士国家試験新全科の要点

    KIKUZAKI Hiroe (, Range: 分担)

    医歯薬出版, Aug. 2006, 154-161, 428-429

  • 食と健康-食品の成分と機能

    KIKUZAKI Hiroe (, Range: 分担)

    放送大学教育振興会, Mar. 2006, 95-107,215-227

  • 栄養・食糧学データハンドブック

    KIKUZAKI Hiroe (, Range: 分担)

    同文書院, Jan. 2006, 191-193

  • 平成17年度大阪府栄養教諭免許法認定講習テキスト-学校栄養教育の理論と実践・栄養教育実習事前指導

    KIKUZAKI Hiroe (, Range: 分担)

    Aug. 2005, 41-50

  • 2006新カリ対応管理栄養士国家試験完全攻略○×チェック実戦4000問

    KIKUZAKI Hiroe (, Range: 分担)

    医歯薬出版, Jul. 2005, 64-66、275-278

  • 生活科学I 食の科学

    KIKUZAKI Hiroe (, Range: 分担)

    放送大学教育振興会, Apr. 2005, 111-128, 200-220, 278-293

  • 読売ファミリー北版8月4日号・疑問解決隊-昆布はなぜ海の中でだしを出さないの

    KIKUZAKI Hiroe (, Range: 監修)

    読売ファミリー編集部, Aug. 2004

  • わたしの食卓3・ハーブとスパイス、本当の使い方を知っていますか?

    KIKUZAKI Hiroe (, Range: 監修)

    毎日新聞社, Aug. 2004, 12-15、24-25、34-35

  • 新版 家政学事典

    KIKUZAKI Hiroe (, Range: 分担)

    朝倉書店, Jul. 2004, 430

  • 食生活研究・野菜の品質特性の変遷と食生活との関り(2)

    KIKUZAKI Hiroe (, Range: 分担)

    食生活研究会, Jul. 2004, 14-21

  • 食生活研究・野菜の品質特性の変遷と食生活との関り(1)

    KIKUZAKI Hiroe (, Range: 分担)

    食生活研究会, May 2004, 13-22

  • 食べ物と健康 調理学

    KIKUZAKI Hiroe (, Range: 分担)

    医歯薬出版, Feb. 2004, 55-56、180-188、205-210、256-259

  • Oriental Foods and Herbs, Chemistry and Health Effects

    KIKUZAKI Hiroe; H. Kikuzaki; authors (, Range: 分担)

    American Chemical Society, Sep. 2003, 176-189

  • 栄養科学シリーズNEXT-調理学実習

    KIKUZAKI Hiroe (, Range: 分担)

    講談社, Apr. 2003

  • スタンダード栄養・食物シリーズ5 食品学-食品成分と機能性

    KIKUZAKI Hiroe (, Range: 分担)

    東京化学同人, Apr. 2003, 70-90、98-100、252-256

  • きょうどう・お料理サイエンス

    KIKUZAKI Hiroe (, Range: 監修)

    コープこうべ, Apr. 2003, 4-5

  • 食と健康-情報のウラを読む

    KIKUZAKI Hiroe (, Range: 分担)

    丸善, Nov. 2002, 173-198

  • 食品学実験書

    KIKUZAKI Hiroe (, Range: 分担)

    医歯薬出版, Aug. 2002, 108-116、132-135、191-196

  • 栄養と健康の専門知識がよくわかる食生活

    KIKUZAKI Hiroe (, Range: 分担)

    全衛連「食生活」出版局, Nov. 2001, 79-83

  • Quality Management of Nutraceuticals

    KIKUZAKI Hiroe; P.A. Lachance; authors (, Range: 分担)

    American Chemical Society, Jan. 2001, 230-240

  • AERA Mook-食生活学がわかる

    KIKUZAKI Hiroe (, Range: 分担)

    朝日新聞社, Nov. 2000, 132-135

  • Herbs, Botanicals & Teas

    KIKUZAKI Hiroe; M. Atha (, Range: 分担)

    Technomic Publishing Co. Inc., Jun. 2000, 75-105

  • 生活科学最前線90のトピック

    KIKUZAKI Hiroe (, Range: 分担)

    中央法規出版, Sep. 1999, 48-49

  • 抗酸化成分のすべて

    KIKUZAKI Hiroe (, Range: 分担)

    先端医学社, Sep. 1998, 148-156

  • 食品総合辞典

    KIKUZAKI Hiroe (, Range: 分担)

    丸善, Mar. 1998

  • 栄養学ハンドブック(第3版)

    KIKUZAKI Hiroe (, Range: 分担)

    技報堂出版, Nov. 1996, 152-154

  • 活性酸素と医食同源

    KIKUZAKI Hiroe (, Range: 分担)

    共立出版, Jul. 1996, 216-219

  • 昭和63年度健康情報調査報告書 香辛料

    KIKUZAKI Hiroe (, Range: 分担)

    健康・体力づくり事業財団, Mar. 1989, 364-391

  • 食品学 : 食品成分と機能性

    久保田, 紀久枝; 森光, 康次郎 (, Range: 第II部 食品の成分 10 嗜好成分-色素成分、呈味成分、注目されている嗜好成分の機能性)

    東京化学同人, Mar. 2021, x, 292p, cinii_books (ISBN: 9784807916832)

  • スパイス・ハーブの機能と応用

    森川, 敏生 (, Range: 第4章4 フトモモ科(オールスパイス、クローブ), pp.121-130)

    シーエムシー出版, Oct. 2020, vii, 353p, cinii_books (ISBN: 9784781315218)

Presentations

  • サバ筋肉中におけるヒスタミン生成を抑制するハーブ・スパイスの探索

    KIKUZAKI Hiroe

    第33回日本香辛料研究会, Nov. 2018, 一般社団法人日本香辛料研究会, 味の素株式会社クライアント・イノベーション・センター、神奈川県

  • 食用植物に含まれるヒスタミン産生抑制成分の探索

    KIKUZAKI Hiroe

    第6回奈良まほろば産学官連携懇話会, Sep. 2018, 奈良まほろば産学官連携懇話会, 奈良女子大学、奈良県

  • 加熱調理がカロテノイド及びポリフェノール系成分由来の抗酸化力に及ぼす影響 人参の煮物の場合

    KIKUZAKI Hiroe

    日本調理科学会平成30年度大会, Aug. 2018, 一般社団法人日本調理科学会, 武庫川女子大学 兵庫県

  • Elucidation of constituents in coriander (Coriandrum sativum L.) leaves and stem based on antioxidant activities

    KIKUZAKI Hiroe

    日本農芸化学会2017年度大会, Mar. 2018, 日本農芸化学会, 名城大学、名古屋

  • さとうきび抽出物に含まれる脂質酸化抑制成分の探索と定量分析による主要成分の解明

    KIKUZAKI Hiroe

    第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, 日本栄養・食糧学会近畿支部, 龍谷大学瀬田キャンパス

  • コリアンダー(Coriandrum sativum L.)の抗酸化性

    KIKUZAKI Hiroe

    第32回日本香辛料研究会, Sep. 2017, 日本香辛料研究会, 兵庫大学

  • ローズレッドペタルに含まれるフラボノイド配糖体のヒスチジン脱炭酸酵素阻害活性と阻害様式

    KIKUZAKI Hiroe

    第71回日本栄養・食糧学会大会, May 2017, 日本栄養・食糧学会, 琉球大学

  • Inhibitory activity of flavonol glycosides in the petal of Rosa gallica on histidine decarboxylase.

    KIKUZAKI Hiroe

    Japanese Society for Food Factors JSoFF2016, Nov. 2016, 日本フードファクター学会, 富山国際会議場 富山市

  • アメリカのFood Serviceテキストと日本の給食経営管理論教科書との比較

    KIKUZAKI Hiroe

    第12回日本給食経営管理学会学術総会, Nov. 2016, 日本給食経営管理学会, 大手前大学 さくら夙川キャンパス 兵庫県西宮市

  • ショウガ科香辛料に含まれる機能物質の探索

    KIKUZAKI Hiroe

    第60回香料・テルペンおよび精油化学に関する討論会, Oct. 2016, 香料・テルペンおよび精油化学に関する討論会, 東京農業大学オホーツクキャンパス 網走市

  • Antioxidative constituents in the sugar cane extract.

    KIKUZAKI Hiroe

    日本油化学会第55回年会, Sep. 2016, 日本油化学会, 奈良女子大学 奈良市

  • The antioxidant capacity of polyphenol-rich foods and dishes. -Influence of vitamin C on the antioxidant capacity-

    KIKUZAKI Hiroe

    日本調理科学会平成28年度大会, Aug. 2016, 日本調理科学会, 名古屋学芸大学 名古屋市

  • メチル化カテキンによる分泌型スフィンゴミエリナーゼ阻害作用

    KIKUZAKI Hiroe

    第70回日本栄養・食糧学会大会, May 2016, 日本栄養・食糧学会, 武庫川女子大学

  • 抗酸化物質による分泌型スフィンゴミエリナーゼ阻害作用

    KIKUZAKI Hiroe

    第9回日本ポリフェノール学会学術大会, Aug. 2015, 日本ポリフェノール学会, 奈良女子大学、奈良市

  • Inhibition of secretory sphingomyelinase by epicatechin 3-O-gallate

    KIKUZAKI Hiroe; Keiko Kobayashi; Yuki Ishizaki; Moe Miyamoto; Hiroe Kikuzaki

    ACN2015 Asian Congress of Nutrition, May 2015, Japan Society of Nutrition and Food Science, Yokohama, Japan

  • Epicatechin gallate inhibits secretory shingomyelinase

    KIKUZAKI Hiroe

    第87回日本生化学会大会, Oct. 2014, 京都国際会館、京都

  • カテキン類による分泌型スフィンゴミエリナーゼの阻害作用に関する研究

    KIKUZAKI Hiroe

    日本家政学会関西支部第36回(通算92回)研究発表会, Oct. 2014, 日本家政学会関西支部, 京都聖母女学院短期大学、京都

  • Inhibitory effects of polyphenols in Rosa gallica on recombinant human histidine decarboxylase

    KIKUZAKI Hiroe; Hiroe Kikuzaki; Kozue Akimoto; Yuka Watanabe; Keiko Kobayashi; Hiroshi Ueno; Yoko Nitta

    XXVIIth International Conference of Polyphenols & 8th Tannin Conference, Sep. 2014, ICP2014, Nagoya University, Nagoya

  • Appropriateness of using antioxidant capacity of food with the ORACmethod for diet management

    KIKUZAKI Hiroe

    日本調理科学会平成26年度大会, Aug. 2014, 日本調理科学会, 広島市 県立広島大学

  • Inhibitory effect of (-)-epigallocatechin gallate on secretory sphingomyelinase

    KIKUZAKI Hiroe

    第68回日本栄養・食糧学会大会, Jun. 2014, 札幌市 酪農学園大学

  • Rosa gallicaの花弁に含まれるヒスチジン脱炭酸酵素阻害活性成分の探索

    KIKUZAKI Hiroe

    日本農芸化学会2014年度大会, Mar. 2014, 神奈川県 明治大学

  • 香辛料の抗酸化機能-オールスパイス-

    KIKUZAKI Hiroe

    第20回スパイス&ハーブ研究成果セミナー, Nov. 2013, 公益財団法人 山崎香辛料振興財団, 東京都 エスビー食品㈱スパイスセンター

  • Change of activity of secretory sphingomyelinase and ceramide levels in apoE-/- mice during aging process

    KIKUZAKI Hiroe

    第86回日本生化学会大会, Sep. 2013, 横浜

  • ORACを指標とした野菜および野菜料理の抗酸化力の評価

    KIKUZAKI Hiroe

    日本調理科学会平成25年度大会, Aug. 2013, 日本調理科学会, 奈良市 奈良女子大学

  • 食品ゲルのテクスチャーとフレーバーリリース\n寒天ゲルからのショ糖のリリース-寒天濃度、ショ糖濃度、ゲル調製法の影響-

    KIKUZAKI Hiroe

    日本食品科学工学会第60回記念大会 シンポジウムB1「食品ゲルのテクスチャーとフレーバーリリース」, Aug. 2013, 東京都 実践女子大学

  • apoE欠損型マウスにおける老化に伴う動脈硬化進行とセラミド動態に関する研究

    KIKUZAKI Hiroe

    第67回日本栄養・食糧学会大会, May 2013, 名古屋市 名古屋大学

  • 酵素グルコシダーゼや腸管モデル培養細胞を用いた配糖体Leptosinの代謝研究

    KIKUZAKI Hiroe

    日本農芸化学会2013年度大会, Mar. 2013, 日本農芸化学会, 東北大学

  • マヌカ蜂蜜から発見された新規配糖体の単離と酵素を用いた構造解析

    KIKUZAKI Hiroe

    第17回日本フードファクター学会学術集会, Nov. 2012, 日本フードファクター学会, 静岡

  • Isoflavone C-glycosides isolated from the root of Kudzu (Pueraria lobata) and their estrogenic activities

    KIKUZAKI Hiroe; Shin-ichi Kayano; Yoko Matsumura; Yoko Kitagawa; Mayumi Kobayashi; Asuka Nagayama; Nami Kawabata; Hiroe Kikuzaki; Yoshimi Kitada

    2012 EFFoST Annual Meeting, Nov. 2012, European Federation of Food Science and Technology, Montpellier, France

  • 給食経営管理実習を利用したエネルギー教育の試み~定格消費電力と作業工程表に基づいた消費電力量の予測と実測~

    KIKUZAKI Hiroe

    第8回日本給食経営管理学会学術総会, Nov. 2012, 日本給食経営管理学会, 名古屋女子大学汐路学舎

  • ショ糖添加寒天ゲルからのショ糖のリリースと甘味強度

    KIKUZAKI Hiroe

    日本調理科学会平成24年度大会, Aug. 2012, 日本調理科学会, 秋田大学

  • ショウガ科植物Kaempferia parviflora根茎に含まれる熱に安定な色素について

    KIKUZAKI Hiroe

    日本調理科学会平成24年度大会, Aug. 2012, 日本調理科学会, 秋田大学

  • Sucrose release from agar gels as studied by uniaxial compression and sensory evaluation

    KIKUZAKI Hiroe; Z. Wang; K. Yang; H. Kikuzaki; H. Moritaka; K. Nishinari

    2nd International Conference of Food Oral Processing, Physics, Physiology and Psychology of Eating, Jul. 2012, the Institut National de la Recherche Agronomique and the Royal Society of Chemistry, Beaune, France

  • 食品ゲルのテクスチャーとフレーバーリリース 1.ショ糖添加寒天ゲルからのショ糖のリリース

    KIKUZAKI Hiroe

    日本家政学会第64回大会, May 2012, 日本家政学会, 大阪市立大学

  • 食品ゲルのテクスチャーとフレーバーリリース 2.ショ糖添加寒天ゲルからのショ糖のリリース

    KIKUZAKI Hiroe

    日本家政学会第64回大会, May 2012, 日本家政学会, 大阪市立大学

  • Kaempferia parvifloraから得られたメトキシフラボンの抗アレルギー作用

    KIKUZAKI Hiroe

    第66回日本栄養・食糧学会大会, May 2012, 仙台市 東北大学

  • マヌカ蜂蜜に由来する新規配糖体Leptosinのケミカルマーカーとしての有用性について

    KIKUZAKI Hiroe

    日本農芸化学会2012年度大会, Mar. 2012, 日本農芸化学会, 京都女子大学

  • 食用植物に含まれる機能性成分の化学構造と活性

    KIKUZAKI Hiroe

    地域イノベーション戦略支援プログラム 和歌山県紀北紀中エリア\n平成23年度第2階研究交流会, Dec. 2011, 公益財団法人わかやま産業振興財団、和歌山県, 和歌山市

  • 給食経営管理実習中のガス・電力消費量の「見える化」および測定によるエネルギー教育の試み

    KIKUZAKI Hiroe

    第7回日本給食経営管理学会学術総会, Nov. 2011, 日本給食経営管理学会, 大妻女子大学千代田キャンパス

  • Novel Chemical Marker of Nanuka Honey

    KIKUZAKI Hiroe; Yoji Kato; Natsuki Umeda; Asuna Maeda; Noritoshi Kitamoto; Hiroe Kikuzaki

    2011 International Conference on Food Factors, Nov. 2011, 2011 International Conference on Food Factors, Taipei, Taiwan

  • さとうきび抽出物による鮭の退色抑制効果

    KIKUZAKI Hiroe

    日本調理科学会平成23年度大会, Aug. 2011, 日本調理科学会, 高崎健康福祉大学

  • 食品ゲルのレオロジー特性とフレーバーリリース

    KIKUZAKI Hiroe

    日本調理科学会平成23年度大会, Aug. 2011, 日本調理科学会, 高崎健康福祉大学

  • 葛デンプン晒し液より単離したイソフラボンのエストロゲン様活性及び抗変異原性の評価

    KIKUZAKI Hiroe

    第65回日本栄養食糧学会大会, May 2011, 日本栄養・食糧学会

  • メドウスウィート(Filipendula ulmaria)成分によるヒスチジン脱炭酸酵素阻害機構の解明

    KIKUZAKI Hiroe

    第25回日本香辛料研究会, Nov. 2010

  • メドウスウィート(Filipendula ulmaria)のヒスチジン脱炭酸酵素阻害成分の探索

    KIKUZAKI Hiroe

    第15回日本フードファクター学会学術集会, Oct. 2010, 仙台

  • タイ原産ナス(Solanum sanitwongsei)の成分

    KIKUZAKI Hiroe

    日本栄養・食糧学会第49回近畿支部大会, Oct. 2010

  • 給食経営管理実習中のガス消費量「見える化」によるエネルギー教育の試み

    KIKUZAKI Hiroe

    第6回日本給食経営管理学会学術総会, Oct. 2010

  • 大阪特産野菜「葉ゴボウ」に含まれる抗酸化成分の定量と抗酸化成分に対する加熱調理の影響

    KIKUZAKI Hiroe

    日本調理科学会平成22年度大会, Aug. 2010

  • ショウガ科植物Kaempferia parvifloraに含まれるフラボノイドの抗変異原性

    KIKUZAKI Hiroe

    第64回日本栄養・食糧学会大会, May 2010

  • 葛デンプン晒し液より単離したイソフラボンC-配糖体の化学構造およびエストロゲン様活性

    KIKUZAKI Hiroe

    第64回日本栄養・食糧学会大会, May 2010

  • Kaempferia parvifloraの抗アレルギー成分

    KIKUZAKI Hiroe

    第64回日本栄養・食糧学会大会, May 2010, 徳島

  • ゲットウ (Alpinia zerumbet (Pers.)Burtt and Smith) 種子に含まれる膵リパーゼ阻害成分の解明

    KIKUZAKI Hiroe

    日本農芸化学会2010年度大会, Mar. 2010

  • マンゴージンジャー(Curcuma amada)に含まれるジテルペノイドの構造

    KIKUZAKI Hiroe

    第53回香料・テルペンおよび精油化学に関する討論会, Nov. 2009

  • オンジ(Polygala tenuifolia)からの1,5-アンヒドログルシトールの精製と単離

    KIKUZAKI Hiroe

    第48回日本栄養・食糧学会近畿支部大会, Nov. 2009

  • ローズマリーに含まれるポリフェノールの抗酸化能

    KIKUZAKI Hiroe

    第14回日本フードファクター学会学術集会, Nov. 2009

  • メドウスウィート(Filipendula ulmaria (L.) Maxim.)に含まれるヒスチジン脱炭酸酵素阻害成分

    KIKUZAKI Hiroe

    第24回日本香辛料研究会, Nov. 2009

  • Multiple Functions in Ginger Family

    KIKUZAKI Hiroe; Hiroe Kikuzaki; Nobuji Nakatani

    Italy-Japan Symposium on "Foods and Health: past, present and future", Oct. 2009, Intituo Italiano di Culture, Tokyo, Japan

  • アレルギー対応クッキーの品質特性-生地を真空包装し冷凍保存した場合-

    KIKUZAKI Hiroe

    第5回日本給食経営管理学会学術総会, Oct. 2009

  • 給食経営管理実習時のガス消費量の「見える化」による省エネ意識の変化

    KIKUZAKI Hiroe

    第5回日本給食経営管理学会学術総会, Oct. 2009

  • 皮茸(コウタケ)の抗酸化性および抗変異原性の評価

    KIKUZAKI Hiroe

    第56回日本栄養改善学会学術総会, Sep. 2009

  • 低アレルゲン大豆「なごみまる」のイソフラボンおよび脂肪酸分析と「なごみまる」を原料とした味噌の官能評価

    KIKUZAKI Hiroe

    日本調理科学会平成21年度大会, Aug. 2009

  • ナス果肉から単離した成分の抗酸化性の定量的評価

    KIKUZAKI Hiroe

    第63回日本栄養・食糧学会大会, May 2009

  • 葛デンプン晒し液より単離したイソフラボンのエストロゲン様活性の評価

    KIKUZAKI Hiroe

    第63回日本栄養・食糧学会大会, May 2009

  • ショウガ科植物Kaempferia parvifloraに含まれる色素の構造と熱安定性

    KIKUZAKI Hiroe

    第23回日本香辛料研究会, Nov. 2008

  • ヒスタミン合成を阻害するオールスパイス成分の探索

    KIKUZAKI Hiroe

    第13回日本フードファクター学会, Nov. 2008

  • スチームコンベクションオーブン加熱における庫内水蒸気濃度が食品の昇温に与える影響

    KIKUZAKI Hiroe

    第4回日本給食経営管理学会学術総会, Nov. 2008

  • Radical scavenging and hyaluronidase inhibitory activities of constituents in Japanese vegetables, Arctiun lappa and Petasites japonicus

    KIKUZAKI Hiroe; Hiroe Kikuzaki; Chiyomi Suzuki; Yuzuri Iwamoto; Nobuji Nakatani

    Italian-Japanese Joint Symposium-Natural products and functional foods, Jun. 2008, Salerono, Italy

  • Search for antioxidants in spices and herbs

    KIKUZAKI Hiroe; Nobuji Nakatani; Hiroe Kikuzaki

    Joint symposium-Natural products and functional foods, Jun. 2008, Salerno, Italy

  • 葛デンプン晒し液から単離したイソフラボンの構造解析および抗変異原性の評価

    KIKUZAKI Hiroe

    第62回日本栄養・食糧学会大会, May 2008

  • 葉ゴボウ(Arctium lappa)のルチンとクロロゲン酸含量

    KIKUZAKI Hiroe

    第62回日本栄養・食糧学会大会, May 2008

  • パプアナツメグ、パプアメース(Myristica argentea)に含まれるリグナンのラジカル捕捉活性評価

    KIKUZAKI Hiroe

    第62回日本栄養・食糧学会大会, May 2008

  • 黒ショウガ(Kaempferia parviflora)根茎に含まれる新規配糖体の構造

    KIKUZAKI Hiroe

    日本薬学会第128年会, Mar. 2008

  • ショウガ科植物に含まれる一酸化窒素産生抑制成分

    KIKUZAKI Hiroe

    日本農芸化学会2008年度大会, Mar. 2008

  • スチームコンベクションオーブンを利用した加熱調理の特性と給食経営管理における有用性の検討

    KIKUZAKI Hiroe

    第3回日本給食経営管理学会学術総会, Nov. 2007

  • スチームコンベクションオーブンの使用形態とエネルギー消費

    KIKUZAKI Hiroe

    第3回日本給食経営管理学会学術総会, Nov. 2007

  • Antimicrobial activity of saponins from leaves of Bacopa monnieri against yeast

    KIKUZAKI Hiroe; Hisamoto, M; Okuda, T; Nishihara, N; Kikuzaki, H; Yokotsuka, K

    International Conference of Food Factors for Health Promotion-ICoFF2007, Nov. 2007

  • Constituents Enhancing eNOS Protein Expression in the Fruits of Piper longum

    KIKUZAKI Hiroe; Hiroe Kikuzaki; Chitose furukawa; Ryoji Takagaki; Yasunaga Yamaguchi; Nobuaki Ohto

    International Conference of Food Factors for Health Promotion-ICoFF2007, Nov. 2007

  • Search for Zingiberaceae Phytochemicals that Suppress NO Generation from Stimulated Macrophages

    KIKUZAKI Hiroe; Amane Takahashi; Norio Yamamoto; Toshiaki Azuma; Kayo Akiyama; Hiroe Kikuzaki; Nobuji Nakatani; Hajime Ohigashi

    International Conference on Food Factors for Health Promotion-ICoFF2007, Nov. 2007

  • Radical Scavenging Capacity of Tannins and Flavonoids Isolated from Salam Leaf (Syzygium polyanthum)

    KIKUZAKI Hiroe; Kayo Akiyama; Nobuji Nakatani; Hiroe Kikuzaki

    ICPH2007-3rd International Conference of Polyphenols and Health, Nov. 2007

  • 大阪産野菜「葉ごぼう」のラジカル捕捉活性について

    KIKUZAKI Hiroe

    日本家政学会関西支部第29回研究発表会, Oct. 2007

  • Synergistic Growth Inhibitory Effect ofConstituents of Cape Aloe Extract in Ehrlich Ascites Tumor Cells

    KIKUZAKI Hiroe; Kametani, S; Kojima-Yuasa, A; Kikuzaki, H; Honzawa, M; Matsui-Yuasa, I

    10th Asian Congress of Nutrition, Aug. 2007

  • 黒ショウガ(Kaempferia parviflora)に含まれるフラボノイドのNO産生抑制活性

    KIKUZAKI Hiroe

    第61回日本栄養・食糧学会大会, May 2007

  • フキ葉身の栄養成分分析

    KIKUZAKI Hiroe

    第61回日本栄養・食糧学会大会, May 2007

  • Constituents of coriander leaves involved in antioxidant and anti-allergic activities

    Rika Ohara; Yurika Kitamura; Takuya Sugahara; Hiroyuki Onda; Nanami Yoshino; Hiroe Kikuzaki

    ICoFF2019, 04 Dec. 2019, 01 Dec. 2019, 05 Dec. 2019

  • The inhibitory activity of ellagitannins on recombinant human histidine decarboxylase

    Yoko Nitta; Hideyuki Ito; Hirohumi Komori; Hiroshi Ueno; Hiroe Kikuzaki

    ICPH2019, 29 Nov. 2019, 28 Nov. 2019, 01 Dec. 2019

  • 葛デンプン晒し液より単離した化合物の化学構造解析およびエストロゲン様活性の評価

    上山蓮実; 菊崎泰枝; 北瓜亮太; 餅田尚子; 松村羊子; 栢野新市

    第73回日本栄養・食糧学会, 18 May 2019

Teaching Experience

  • Organic Chemistry I (Nara Women's University)

  • Practical Training in Food Service Management (Nara Women's University)

  • Practice of Food Service Management (Nara Women's University)

  • Food Service Magagement II (Nara Women's University)

  • Food Service Management I (Nara Women's University)

Association Memberships

  • 日本農芸化学会

  • 日本栄養・食糧学会

  • 日本調理科学会

  • 日本家政学会

  • 日本給食経営管理学会

  • 日本栄養改善学会

  • 日本油化学会

  • 日本薬学会

  • 日本生薬学会

  • 日本フードファクター学会

  • 日本香辛料研究会

  • 植物化学研究会

  • 香料・テルペンおよび精油化学に関する討論会

  • AOU研究会

  • 抗酸化・機能研究会

Works

  • さとうきび由来成分による食品中の油脂酸化抑制に関する研究

    Jun. 2014, Mar. - 2015

  • さとうきび由来成分による食品中の油脂酸化抑制に関する研究

    Jun. 2013, Mar. - 2014

  • ごぼうの有効成分の単離、同定に関する研究

    Jun. 2012, Mar. - 2013

  • さとうきび由来成分による食品への抗酸化作用に関する研究

    Jun. 2012, Mar. - 2013

  • さとうきび抽出物の成分解明及び食品に対する抗酸化作用に関する研究

    Jun. 2011, Mar. - 2012

  • さとうきび抽出物の抗酸化性について

    Jun. 2009, Mar. - 2010

  • アレルギー除去食品の開発及び幼稚園献立の監修

    Aug. 2006, Jul. - 2007

  • サクラダソウの成分分析

    Apr. 2006, Mar. - 2007

  • ヒハツの機能性成分の解明

    2005, - 2007

  • マンゴージンジャーの機能性成分の解明

    2004, - 2007

  • 化粧品原料ローズマリーエキスの成分分析

    Jan. 2006, Jun. - 2006

  • カワラヨモギの機能成分の解析

    Jul. 2004, Jun. - 2005

  • 黒ショウガ成分の構造解明

    Jul. 2004, Jun. - 2005

  • 竹に含まれる機能成分の解明

    Jun. 2004, May - 2005

  • プルーン(Prunus domestica L.)に含まれる生理活性物質の精査研究

    Nov. 2003, Oct. - 2004



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