Yoshimoto MIsa

Nara Women's University, Mar. 2005

Life sciences, Nutrition and health science

Life sciences, Animals: biochemistry, physiology, behavioral science

Apr. 2023, 9999, Nara Women's University, Faculty, Division of Human Life and Environmental Sciences, 教授

日本生理学会, 2001, 9999

Experimental physiology, Simultaneous measurement of central amygdala neuronal activity and sympathetic nerve activity during daily activities in rats., Shizuka Ikegame; Misa Yoshimoto; Kenju Miki, NEW FINDINGS: What is the central question of this study? The functional relationships between central amygdala neuronal activity (CeANA) and sympathetic nerve activity in daily activities remain unclear. We aimed to measure CeANA, renal and lumbar sympathetic nerve activity (RSNA and LSNA, respectively), heart rate (HR) and arterial pressure simultaneously in freely moving rats. What is the main finding and its importance? The CeANA was significantly related to RSNA and LSNA and HR in a behavioural state-dependent and regionally different manner; meanwhile, CeANA was tightly associated with RSNA and HR across all behavioural states. Thus, it is likely that the amygdala is a component of neural networks generating regional differences in RSNA and LSNA. ABSTRACT: The central amygdala (CeA) is involved in generating diverse changes in sympathetic nerve activity (SNA) in response to changes in daily behavioural states. However, the functional relationships between CeA neuronal activity (CeANA) and SNA in daily activities are still unclear. In the present study, we developed a method for simultaneous and continuous measurement of CeANA and SNA in freely moving rats. Wistar rats were chronically instrumented with multiple electrodes (100-μm-thick stainless-steel wire) for the measurement of CeANA, renal SNA (RSNA) and lumbar SNA (LSNA), and electroencephalogram, EMG and ECG electrodes, in addition to catheters for measurement of arterial pressure (AP). During the transition from non-rapid eye movement sleep to quiet wakefulness, moving and grooming states, a significant linear relationship was observed between CeANA and RSNA (P < 0.0001), between CeANA and LSNA (P = 0.0309), between CeANA and heart rate (HR) (P = 0.0123) and between CeANA and EMG (P = 0.0089), but no significant correlation was observed between CeANA and AP (P = 0.5139). During rapid eye movement sleep, the relationships between CeANA and RSNA, LSNA, HR, AP and EMG deviated from the previously observed linear relationships, but the time course of RSNA and HR changes was the mirror image of that of CeANA, whereas the time course of changes in LSNA and AP was not related to that of CeANA. In conclusion, CeANA was related to RSNA, LSNA and HR in a behavioural state-dependent and regionally different manner, and CeANA was tightly associated with RSNA and HR across all behavioural states., Sep. 2022, 107, 9, 1071, 1080, Scientific journal, True, 10.1113/EP090538

Refereed, Experimental Physiology, Wiley, Differential shifts in baroreflex control of renal and lumbar sympathetic nerve activity induced by freezing behaviour in rats, Naomi Kondo; Misa Yoshimoto; Shizuka Ikegame; Kenju Miki, NEW FINDINGS: What is the central question of this study? Is the arterial baroreflex involved in causing patterned, region-specific changes in sympathetic nerve activity during freezing behaviour in conscious rats? What is the main finding and its importance? Freezing behaviour is accompanied by differential shifts in the baroreflex control of renal and lumbar sympathetic nerve activity and heart rate. It is noteworthy that baroreflex pathways may be discretely separated, allowing differential modification of baroreflex curves that may generate differential changes in sympathetic nerve activity during freezing behaviour. ABSTRACT: The present study was designed to test whether the baroreflex stimulus-response curves for renal sympathetic nerve activity (RSNA), lumbar sympathetic nerve activity (LSNA) and heart rate (HR) were shifted in a regionally specific manner during freezing behaviour in conscious rats. Male Wistar rats were chronically instrumented with electrodes and arterial and venous catheters for measurement of RSNA, LSNA and electrocardiogram. After a 60-min control period, freezing behaviour in conscious rats was induced by exposure to loud white noise (90 dB) for 10 min. The baroreflex curves for RSNA, LSNA and HR were generated by changing systemic arterial pressure using rapid intravenous infusions of vasoactive drugs and then fitted to an inverse sigmoid function curve. During the freezing behaviour, the baroreflex curve for RSNA was expanded upward with a significant (P < 0.001) increase (by 153% compared with the control level) in the upper plateau (maximum capacity of RSNA drive), whereas the baroreflex curve for LSNA remained unchanged. Conversely, the baroreflex curve for HR was shifted leftward with a significant (P = 0.004) decrease (by 11 mmHg relative to the control level) in the midpoint pressure. Our results indicate that baroreflex curve shifts for RSNA, LSNA and HR occur in a regionally specific manner during freezing behaviour. This indicates that baroreflex pathways may be discretely separated, allowing differential modification of baroreflex curves that may generate differential changes in sympathetic nerve activity during freezing behaviour., Oct. 2021, 106, 10, 2060, 2069, Scientific journal, True, 10.1113/ep089742

Refereed, Hypertension (Dallas, Tex. : 1979), Renal and Lumbar Sympathetic Nerve Activity During Development of Hypertension in Dahl Salt-Sensitive Rats., Misa Yoshimoto; Yuko Onishi; Naoko Mineyama; Shizuka Ikegame; Mikiyasu Shirai; John W Osborn; Kenju Miki, To study the contribution of sympathetic nerve activity (SNA) to the development of hypertension, experiments were designed to continuously and simultaneously measure renal (RSNA) and lumbar SNA (LSNA) during the development of hypertension induced by 8% salt loading in Dahl salt-sensitive (DS) rats. Male DS and salt-resistant rats were instrumented with bipolar electrodes to record RSNA and LSNA and a telemeter to record arterial pressure (AP). AP increased during the first 3 days after the onset of salt loading by ≈10 mm Hg in both DS and Dahl salt-resistant rats. AP continued to increase progressively from day 4 to day 14 of salt loading by 33±1 mm Hg in DS rats, while it remained the same in Dahl salt-resistant rats. RSNA and LSNA increased in the initial few days by 6% to 8%, and decreased gradually thereafter, suggesting that increases in neither RSNA nor LSNA are directly linked with the progressive increase in AP induced by salt loading in DS rats. After the cessation of salt loading, AP pressure returned to the presalt loading level in both DS and Dahl salt-resistant rats. RSNA increased significantly by 32±3% after the cessation of salt loading, while LSNA remained the same in DS rats, suggesting that salt-sensitive mechanisms respond to a loss of sodium, not a gain, and selectively activate RSNA in DS rats. In summary, RSNA and LSNA are not likely to be a primary trigger to initiate the progressive increase in AP induced by 8% salt loading in DS rats., Oct. 2019, 74, 4, 888, 895, Scientific journal, True, 10.1161/HYPERTENSIONAHA.119.12866

Refereed, Endocrinology, Ghrelin Preserves Ischemia-Induced Vasodilation of Male Rat Coronary Vessels Following β-Adrenergic Receptor Blockade., James T Pearson; Nicola Collie; Regis R Lamberts; Tadakatsu Inagaki; Misa Yoshimoto; Keiji Umetani; Philip Davis; Gerard Wilkins; Pete P Jones; Mikiyasu Shirai; Daryl O Schwenke, Acute myocardial infarction (MI) triggers an adverse increase in cardiac sympathetic nerve activity (SNA). Whereas β-adrenergic receptor (β-AR) blockers are routinely used for the management of MI, they may also counter β-AR-mediated vasodilation of coronary vessels. We have reported that ghrelin prevents sympathetic activation following MI. Whether ghrelin modulates coronary vascular tone following MI, either through the modulation of SNA or directly as a vasoactive mediator, has never been addressed. We used synchrotron microangiography to image coronary perfusion and vessel internal diameter (ID) in anesthetized Sprague-Dawley rats, before and then again 30 minutes after induction of an MI (left coronary artery ligation). Rats were injected with either saline or ghrelin (150 µg/kg, subcutaneously), immediately following the MI or sham surgery. Coronary angiograms were also recorded following β-AR blockade (propranolol, 2 mg/kg, intravenously). Finally, wire myography was used to assess the effect of ghrelin on vascular tone in isolated human internal mammary arteries (IMAs). Acute MI enhanced coronary perfusion to nonischemicregions through dilation of small arterioles (ID 50 to 250 µm) and microvessel recruitment, irrespective of ghrelin treatment. In ghrelin-treated rats, β-AR blockade did not alter the ischemia-induced vasodilation, yet in saline-treated rats, β-AR blockade abolished the vasodilation of small arterioles. Finally, ghrelin caused a dose-dependent vasodilation of IMA rings (preconstricted with phenylephrine). In summary, this study highlights ghrelin as a promising adjunct therapy that can be used in combination with routine β-AR blockade treatment for preserving coronary blood flow and cardiac performance in patients who suffer an acute MI., 01 Apr. 2018, 159, 4, 1763, 1773, Scientific journal, True, 10.1210/en.2017-03070

Refereed, Frontiers in neuroscience, Frontiers Media SA, Exercise-Induced Modulation of Baroreflex Control of Sympathetic Nerve Activity., Kenju Miki; Misa Yoshimoto, Exercise modulates arterial pressure (AP) regulation over various time spans. AP increases at the onset of exercise and this increase is then sustained during exercise. Once exercise is stopped, AP is suppressed for up to an hour afterwards. Prolonged endurance training is associated with dysfunction of the sympathetic regulation of AP in response to posture changes (orthostatic intolerance). Baroreflex control of sympathetic nerve activity (SNA) has been extensively studied to understand the mechanisms underlying exercise-induced changes in AP. We have previously presented entire baroreflex AP-SNA curves during and after exercise, and during central volume expansion, obtained using direct measurements of renal sympathetic nerve activity (RSNA) in conscious animals. In this review, we describe the modulatory effects of exercise on baroreflex control of AP based on these entire AP-RSNA baroreflex curves. We suggest that both acute and chronic exercise can have modulatory effects on the entire baroreflex curve for SNA, and that these effects differ among time periods., 2018, 12, 493, 493, Scientific journal, True, 10.3389/fnins.2018.00493

Refereed, Scientific reports, Widespread Coronary Dysfunction in the Absence of HDL Receptor SR-B1 in an Ischemic Cardiomyopathy Mouse Model., James T Pearson; Misa Yoshimoto; Yi Ching Chen; Rohullah Sultani; Amanda J Edgley; Hajime Nakaoka; Makoto Nishida; Keiji Umetani; Mark T Waddingham; Hui-Ling Jin; Yuan Zhang; Darren J Kelly; Daryl O Schwenke; Tadakatsu Inagaki; Hirotsugu Tsuchimochi; Issei Komuro; Shizuya Yamashita; Mikiyasu Shirai, Reduced clearance of lipoproteins by HDL scavenger receptor class B1 (SR-B1) plays an important role in occlusive coronary artery disease. However, it is not clear how much microvascular dysfunction contributes to ischemic cardiomyopathy. Our aim was to determine the distribution of vascular dysfunction in vivo in the coronary circulation of male mice after brief exposure to Paigen high fat diet, and whether this vasomotor dysfunction involved nitric oxide (NO) and or endothelium derived hyperpolarization factors (EDHF). We utilised mice with hypomorphic ApoE lipoprotein that lacked SR-B1 (SR-B1-/-/ApoER61h/h, n = 8) or were heterozygous for SR-B1 (SR-B1+/-/ApoER61h/h, n = 8) to investigate coronary dilator function with synchrotron microangiography. Partially occlusive stenoses were observed in vivo in SR-B1 deficient mice only. Increases in artery-arteriole calibre to acetylcholine and sodium nitroprusside stimulation were absent in SR-B1 deficient mice. Residual dilation to acetylcholine following L-NAME (50 mg/kg) and sodium meclofenamate (3 mg/kg) blockade was present in both mouse groups, except at occlusions, indicating that EDHF was not impaired. We show that SR-B1 deficiency caused impairment of NO-mediated dilation of conductance and microvessels. Our findings also suggest EDHF and prostanoids are important for global perfusion, but ultimately the loss of NO-mediated vasodilation contributes to atherothrombotic progression in ischemic cardiomyopathy., 22 Dec. 2017, 7, 1, 18108, 18108, Scientific journal, True, 10.1038/s41598-017-18485-6

Refereed, American journal of physiology. Regulatory, integrative and comparative physiology, Chronic intermittent hypoxia accelerates coronary microcirculatory dysfunction in insulin-resistant Goto-Kakizaki rats., Yi Ching Chen; Tadakatsu Inagaki; Yutaka Fujii; Daryl O Schwenke; Hirotsugu Tsuchimochi; Amanda J Edgley; Keiji Umetani; Yuan Zhang; Darren J Kelly; Misa Yoshimoto; Hisashi Nagai; Roger G Evans; Ichiro Kuwahira; Mikiyasu Shirai; James T Pearson, Chronic intermittent hypoxia (IH) induces oxidative stress and inflammation, which impair vascular endothelial function. Long-term insulin resistance also leads to endothelial dysfunction. We determined, in vivo, whether the effects of chronic IH and insulin resistance on endothelial function augment each other. Male 12-wk-old Goto-Kakizaki (GK) and Wistar control rats were subjected to normoxia or chronic IH (90-s N2, 5% O2 at nadir, 90-s air, 20 cycles/h, 8 h/day) for 4 wk. Coronary endothelial function was assessed using microangiography with synchrotron radiation. Imaging was performed at baseline, during infusion of acetylcholine (ACh, 5 μg·kg(-1)·min(-1)) and then sodium nitroprusside (SNP, 5 μg·kg(-1)·min(-1)), after blockade of both nitric oxide (NO) synthase (NOS) with N(ω)-nitro-l-arginine methyl ester (l-NAME, 50 mg/kg) and cyclooxygenase (COX, meclofenamate, 3 mg/kg), and during subsequent ACh. In GK rats, coronary vasodilatation in response to ACh and SNP was blunted compared with Wistar rats, and responses to ACh were abolished after blockade. In Wistar rats, IH blunted the ability of ACh or SNP to increase the number of visible vessels. In GK rats exposed to IH, neither ACh nor SNP were able to increase visible vessel number or caliber, and blockade resulted in marked vasoconstriction. Our findings indicate that IH augments the deleterious effects of insulin resistance on coronary endothelial function. They appear to increase the dependence of the coronary microcirculation on NO and/or vasodilator prostanoids, and greatly blunt the residual vasodilation in response to ACh after blockade of NOS/COX, presumably mediated by endothelium-derived hyperpolarizing factors., 01 Aug. 2016, 311, 2, R426-39, Scientific journal, True, 10.1152/ajpregu.00112.2016

Refereed, Free radical research, Monoamine oxidase-induced hydroxyl radical production and cardiomyocyte injury during myocardial ischemia-reperfusion in rats., Tadakatsu Inagaki; Tsuyoshi Akiyama; Cheng-Kun Du; Dong-Yun Zhan; Misa Yoshimoto; Mikiyasu Shirai, To elucidate the involvement of monoamine oxidase (MAO) in hydroxyl radical production and cardiomyocyte injury during ischemia as well as after reperfusion, we applied microdialysis technique to the heart of anesthetized rats. Dialysate samples were collected during 30 min of induced ischemia followed by 60 min of reperfusion. We monitored dialysate 3,4-dihydrobenzoic acid (3,4-DHBA) concentration as an index of hydroxyl radical production using a trapping agent (4-hydroxybenzoic acid), and dialysate myoglobin concentration as an index of cardiomyocyte injury in the ischemic region. The effect of local administration of a MAO inhibitor, pargyline, was investigated. Dialysate 3,4-DHBA concentration increased from 1.9 ± 0.5 nM at baseline to 3.5 ± 0.7 nM at 20-30 min of occlusion. After reperfusion, dialysate 3,4-DHBA concentration further increased reaching a maximum (4.5 ± 0.3 nM) at 20-30 min after reperfusion, and stabilized thereafter. Pargyline suppressed the averaged increase in dialysate 3,4-DHBA concentration by ∼72% during occlusion and by ∼67% during reperfusion. Dialysate myoglobin concentration increased from 235 ± 60 ng/ml at baseline to 1309 ± 298 ng/ml at 20-30 min after occlusion. After reperfusion, dialysate myoglobin concentration further increased reaching a peak (5833 ± 1017 ng/ml) at 10-20 min after reperfusion, and then declined. Pargyline reduced the averaged dialysate myoglobin concentration by ∼56% during occlusion and by ∼41% during reperfusion. MAO plays a significant role in hydroxyl radical production and cardiomyocyte injury during ischemia as well as after reperfusion., Jun. 2016, 50, 6, 645, 53, Scientific journal, True, 10.3109/10715762.2016.1162300

Refereed, Autonomic neuroscience : basic & clinical, Role of cardiac sympathetic nerves in blood pressure regulation., Erica A Wehrwein; Misa Yoshimoto; Pilar Guzman; Amit Shah; David L Kreulen; John W Osborn, Stellate ganglionectomy (SGx) was used to assess the contribution of cardiac sympathetic nerves to neurogenic hypertension in deoxycorticosterone (DOCA)-salt treated rats. Experiments were conducted in two substrains of Sprague-Dawley (SD) rats since previous studies reported bradycardia in Charles River-SD (CR-SD) rats and tachycardia in SASCO-SD (SA-SD) rats with DOCA treatment suggesting different underlying neural mechanisms. Uninephrectomized male rats underwent SGx or SHAM surgery and were instrumented for telemetric monitoring of mean arterial pressure (MAP) and heart rate (HR). After recovery, 0.9% saline solution and DOCA (50mg) were administered. Baseline MAP (Days 0-5 average) after SGx in CR-SD rats (96±2mmHg; n=7) was not significantly different (p=0.08) than CR-SD SHAM rats (103±3mmHg; n=9); however, there was a significantly lower HR during the baseline period (377±7 vs. 432±7bpm, p<0.05) in SGx rats. In SA-SD rats baseline MAP was not different between SGx and SHAM rats and HR was lower in SGx rats (428±8 vs. 371±5bpm, p<0.05). After DOCA treatment in both substrains, MAP and HR were elevated similarly in SHAM and SGx groups showing minimal impact in both groups of SGx on hypertension development. However, overall MAP in SA-SD SHAM rats reached a significantly higher level (155±10mmHg vs 135±5mmHg, p<0.05) than that observed in CR-SD SHAM rats demonstrating that the magnitude of hypertensive response to DOCA-salt treatment varies between substrains. In conclusion, removal of cardiac sympathetic nerves did not alter the development or maintenance of DOCA-salt hypertension in SD rats., Jul. 2014, 183, 30, 5, Scientific journal, True, 10.1016/j.autneu.2014.02.005

Refereed, Basic research in cardiology, Pulmonary vascular tone is dependent on the central modulation of sympathetic nerve activity following chronic intermittent hypoxia., Mikiyasu Shirai; Hirotsugu Tsuchimochi; Hisashi Nagai; Emily Gray; James T Pearson; Takashi Sonobe; Misa Yoshimoto; Tadakatsu Inagaki; Yutaka Fujii; Keiji Umetani; Ichiro Kuwahira; Daryl O Schwenke, Chronic intermittent hypoxia (IH) provokes a centrally mediated increase in sympathetic nerve activity (SNA). Although this sympathetic hyperexcitation has been linked to systemic hypertension, its effect on the pulmonary vasculature is unclear. This study aimed to assess IH-mediated sympathetic excitation in modulating pulmonary vasculature tone, particularly acute hypoxia vasoconstrictor response (HPV), and the central β-adrenergic signaling pathway for facilitating the increase in SNA. Sprague-Dawley rats were exposed to IH (cycle of 4% O2 for 90 s/air for 90 s) for 8 h/day for 6 weeks. Subsequently, rats were anesthetized and either pulmonary SNA was recorded (electrophysiology), or the pulmonary vasculature was visualized using microangiography. Pulmonary sympathetic and vascular responses to acute hypoxia were assessed before and after central β1-adrenergic receptor blockade (Metoprolol, 200 nmol i.c.v.). Chronic IH increased baseline SNA (110% increase), and exacerbated the sympathetic response to acute hypoxia. Moreover, the magnitude of HPV in IH rats was blunted compared to control rats (e.g., 10 and 20% vasoconstriction, respectively). In only the IH rats, β1-receptor blockade with metoprolol attenuated the hypoxia-induced increase in pSNA and exacerbated the magnitude of acute HPV, so that both sympathetic and HPV responses were similar to that of control rats. Interestingly, the expression of β1-receptors within the brainstem was similar between both control and IH rats. These results suggest that the centrally mediated increase in SNA following IH acts to blunt the local vasoconstrictor effect of acute hypoxia, which reflects an inherent difference between vasodilator and vasoconstrictor actions of SNA in pulmonary and systemic circulations., 2014, 109, 5, 432, 432, Scientific journal, True, 10.1007/s00395-014-0432-y

Refereed, Journal of synchrotron radiation, Assessment of the serotonin pathway as a therapeutic target for pulmonary hypertension., Emily A Gray; Hirotsugu Tsuchimochi; James T Pearson; Takashi Sonobe; Yutaka Fujii; Misa Yoshimoto; Keiji Umetani; Mikiyasu Shirai; Daryl O Schwenke, Blockade of the serotonin reuptake transporter (5-HTT), using fluoxetine, has been identified as a potential therapeutic target for preventing and, importantly, reversing pulmonary hypertension (PH). This study utilized synchrotron radiation microangiography to determine whether fluoxetine could prevent or reverse endothelial dysfunction and vessel rarefaction, which underpin PH. PH was induced by a single injection of monocrotaline (MCT; 60 mg kg(-1)). Following MCT administration, rats received daily injections of either saline or fluoxetine (MCT+Fluox; 10 mg kg(-1)) for three weeks. A third group of rats also received the fluoxetine regime, but only three weeks after MCT (MCT+FluoxDelay). Control rats received daily injections of saline. Pulmonary microangiography was performed to assess vessel branching density and visualize dynamic changes in vessel diameter following (i) acute fluoxetine or (ii) acetylcholine, sodium nitroprusside, BQ-123 (ET-1A receptor blocker) and L-NAME (NOS inhibitor). Monocrotaline induced PH that was inevitably terminal. `Delayed' treatment of fluoxetine (MCT+FluoxDelay) was unable to reverse the progression of PH. Early fluoxetine treatment pre-PH (i.e. MCT+Fluox) attenuated but did not completely prevent vascular remodeling, vessel rarefaction and an increase in pulmonary pressure, and it did not prevent pulmonary endothelial dysfunction. Interestingly, fluoxetine treatment did counter-intuitively prevent the onset of right ventricular hypertrophy. Using synchrotron radiation microangiography, selective blockade of the serotonin reuptake transporter alone is highlighted as not being sufficient to prevent pulmonary endothelial dysfunction, which is the primary instigator for the inevitable onset of vascular remodeling and vessel rarefaction. Accordingly, potential therapeutic strategies should aim to target multiple pathways to ensure an optimal outcome., Sep. 2013, 20, Pt 5, 756, 64, Scientific journal, True, 10.1107/S0909049513021213

JPFSM, The Japanese Society of Physical Fitness and Sports Medicine, Shifts in the baroreflex control of sympathetic nerve activity induced by exercise, MIKI Kenju; YOSHIMOTO Misa, Exercise causes parallel increases in systemic arterial pressure (AP), heart rate (HR) and renal sympathetic nerve activity (RSNA). This review focused on the potential role of the acute shift in the baroreflex control of RSNA in both increasing and stabilizing AP during exercise, and causing hypotension afterwards. Treadmill exercise shifted the baroreflex curve for RSNA acutely to the right and upward, characterized by a significant increase in the maximum response, about 170%, which could well explain the parallel increases in AP, HR, and RSNA. In contrast, exercise shifted the baroreflex stimulus-response curve for HR upwards in rats, differing from the shift shown for RSNA, suggesting that the dependent variable of baroreflex control has to be specified when shifts in baroreflex stimulus response curves are discussed. During the post-exercise period, the AP-RSNA baroreflex curve was suppressed vertically, with a significant reduction of about 50% shown in the upper plateau without any alteration in the minimum response, which may be the reason for the post exercise hypotension. The loading of cardiopulmonary baroreceptors modulated the baroreflex control of RSNA in a way resembling "Flip-Flop" or "On-Off" type regulation. In part, this may explain the orthostatic intolerance caused by endurance training., 25 Aug. 2013, 2, 3, 319, 324, 10.7600/jpfsm.2.319

Refereed, Cardiovascular diabetology, Acute Rho-kinase inhibition improves coronary dysfunction in vivo, in the early diabetic microcirculation., James T Pearson; Mathew J Jenkins; Amanda J Edgley; Takashi Sonobe; Mandar Joshi; Mark T Waddingham; Yutaka Fujii; Daryl O Schwenke; Hirotsugu Tsuchimochi; Misa Yoshimoto; Keiji Umetani; Darren J Kelly; Mikiyasu Shirai, OBJECTIVES: Activation of RhoA/Rho-kinase (ROCK) is increasingly implicated in acute vasospasm and chronic vasoconstriction in major organ systems. Therefore we aimed to ascertain whether an increase in ROCK activity plays a role in the deterioration of coronary vascular function in early stage diabetes. METHODS: Synchrotron radiation microangiography was used to determine in vivo coronary responses in diabetic (3 weeks post streptozotocin 65 mg/kg ip) and vehicle treated male Sprague-Dawley rats (n = 8 and 6). Changes in vessel number and calibre during vasodilator stimulation before and after blockade of nitric oxide synthase and cyclooxygenase were compared between rats. Acute responses to ROCK inhibitor, fasudil (10 mg/kg iv) was evaluated. Further, perivascular and myocardial fibrosis, arterial intimal thickening were assessed by histology, and capillary density, nitrotyrosine and ROCK1/2 expressions were evaluated by immunohistochemical staining. RESULTS: Diabetic rats had significantly elevated plasma glucose (P < 0.001 vs control), but did not differ in fibrotic scores, media to lumen ratio, capillary density or baseline visible vessel number or calibre. Responses to acetylcholine and sodium nitroprusside stimulation were similar between groups. However, in comparison to control rats the diabetic rats showed more segmental constrictions during blockade, which were not completely alleviated by acetylcholine, but were alleviated by fasudil. Further, second order vessel branches in diabetic rats were significantly more dilated relative to baseline (37% vs 12% increase, P < 0.05) after fasudil treatment compared to control rats, while visible vessel number increased in both groups. ROCK2 expression was borderline greater in diabetic rat hearts (P < 0.053). CONCLUSIONS: We found that ahead of the reported decline in coronary endothelial vasodilator function in diabetic rats there was moderate elevation in ROCK expression, more widespread segmental constriction when nitric oxide and prostacyclin production were inhibited and notably, increased calibre in second and third order small arteries-arterioles following ROCK inhibition. Based on nitrotyrosine staining oxidative stress was not significantly elevated in early diabetic rats. We conclude that tonic ROCK mediated vasoconstriction contributes to coronary vasomotor tone in early diabetes., 01 Aug. 2013, 12, 111, 111, Scientific journal, True, 10.1186/1475-2840-12-111

Refereed, Autonomic neuroscience : basic & clinical, Reprint of "Sympathetic nerve activity during sleep, exercise, and mental stress"., Kenju Miki; Misa Yoshimoto, This brief review describes recent findings on the differential regulation of sympathetic nerve activity and its role in regulating systemic arterial pressure during rapid eye-movement sleep, non-rapid-eye movement sleep, exercise and freezing behavior (mental stress). We describe the mechanisms underlying the differential regulation of sympathetic outflows and how they act in concert to orchestrate adjustments of cardiovascular function for the whole body, which are optimized to match changes in organ activity in daily activity., Apr. 2013, 175, 1-2, 70, 5, Scientific journal, True, 10.1016/j.autneu.2013.04.001

Refereed, Autonomic neuroscience : basic & clinical, Sympathetic nerve activity during sleep, exercise, and mental stress., Kenju Miki; Misa Yoshimoto, This brief review describes recent findings on the differential regulation of sympathetic nerve activity and its role in regulating systemic arterial pressure during rapid eye-movement sleep, non-rapid-eye movement sleep, exercise and freezing behavior (mental stress). We describe the mechanisms underlying the differential regulation of sympathetic outflows and how they act in concert to orchestrate adjustments of cardiovascular function for the whole body, which are optimized to match changes in organ activity in daily activity., Mar. 2013, 174, 1-2, 15, 20, Scientific journal, True, 10.1016/j.autneu.2012.12.007

Refereed, Sleep, Functional role of diverse changes in sympathetic nerve activity in regulating arterial pressure during REM sleep., Misa Yoshimoto; Ikue Yoshida; Kenju Miki, STUDY OBJECTIVES: This study aimed to investigate whether REM sleep evoked diverse changes in sympathetic outflows and, if so, to elucidate why REM sleep evokes diverse changes in sympathetic outflows. MEASUREMENTS: Male Wistar rats were chronically implanted with electrodes to measure renal (RSNA) and lumbar sympathetic nerve activity (LSNA), electroencephalogram, electromyogram, and electrocardiogram, and catheters to measure systemic arterial and central venous pressure; these parameters were measured simultaneously and continuously during the sleep-awake cycle in the same rat. RESULTS: REM sleep resulted in a step reduction in RNSA by 36.1% ± 2.7% (P < 0.05), while LSNA increased in a step manner by 15.3% ± 2% (P < 0.05) relative to the NREM level. Systemic arterial pressure increased gradually (P < 0.05), while heart rate decreased in a step manner (P < 0.05) during REM sleep. In contrast to REM sleep, RSNA, LSNA, systemic arterial pressure, and heart rate increased in a unidirectional manner associated with increases in physical activity levels in the order from NREM sleep, quiet awake, moving, and grooming state. Thus, the relationship between RSNA vs. LSNA and systemic arterial pressure vs. heart rate observed during REM sleep was dissociated compared with that obtained during the other behavioral states. CONCLUSIONS: It is suggested that the diverse changes in sympathetic outflows during REM sleep may be needed to increase systemic arterial pressure by balancing vascular resistance between muscles and vegetative organs without depending on the heart., 01 Aug. 2011, 34, 8, 1093, 101, Scientific journal, True, 10.5665/SLEEP.1168

Refereed, Journal of applied physiology (Bethesda, Md. : 1985), Imaging of the closed-chest mouse pulmonary circulation using synchrotron radiation microangiography., Takashi Sonobe; Daryl O Schwenke; James T Pearson; Misa Yoshimoto; Yutaka Fujii; Keiji Umetani; Mikiyasu Shirai, Structural and functional changes of pulmonary circulation related to pathophysiology of pulmonary arterial hypertension (PAH) remain to be fully elucidated. Angiographic visualization in in vivo animals provided a powerful tool for assessing the major indexes associated with the pathogenesis of PAH. In this study, we have exploited the full potential of synchrotron radiation (SR) microangiography to show the ability to visualize pulmonary hemodynamics in a closed-chest mouse. Male adult mice were anesthetized and cannulated with a customized 24-gauge catheter into the right ventricle via the jugular vein for administering iodine contrast agent. The microangiography was performed on the left lung. We measured dynamic changes in vessel diameter in response to acetylcholine (ACh) and acute exposure to hypoxic gas (10% O(2)). Moreover, the pulmonary transit time was estimated by the time of contrast agent circulating. We were able to visualize the pulmonary arteries from the left pulmonary artery (LPA) to the third generation of branching (inner diameter <100 μm). ACh and acute hypoxia induced vascular responses chiefly in the second and third branching vessels rather than the LPA and the first branching vessels. The transit time was only 0.83 s. These results demonstrate the effectiveness of SR for visualizing the pulmonary circulation in a closed-chest mouse. Future studies using SR microangiography on specific gene-targeted knockout and transgenic mice will provide new insights into the pathophysiology of pulmonary dysfunction and functional adaptation to survive in hypoxic condition., Jul. 2011, 111, 1, 75, 80, Scientific journal, True, 10.1152/japplphysiol.00205.2011

Refereed, American journal of physiology. Regulatory, integrative and comparative physiology, Differential control of renal and lumbar sympathetic nerve activity during freezing behavior in conscious rats., Misa Yoshimoto; Keiko Nagata; Kenju Miki, The present study was designed to document changes in sympathetic nerve activity and cardiovascular function when conscious rats were challenged with a noise stressor to induce freezing behavior. The potential contribution of the arterial baroreceptors in regulating sympathetic nerve activity and cardiovascular adjustments during the freezing behavior was then examined. Wistar male rats were assigned to sham-operated (SO) and sinoaortic-denervated (SAD) groups and instrumented chronically with electrodes for measurements of renal (RSNA) and lumbar (LSNA) sympathetic nerve activity, electroencephalogram, electromyogram, and electrocardiogram and catheters for measurements of systemic arterial and central venous pressure. Both SO and SAD rats were exposed to 90 dB of white noise for 10 min, causing freezing behavior in both groups. In SO rats, freezing behavior was associated with an immediate and significant (P < 0.05) increase in RSNA, no changes in LSNA or mean arterial pressure, and a significant (P < 0.05) decrease in heart rate. SAD attenuated the magnitude of the immediate increase in RSNA and had no influence on the response in LSNA during freezing behavior compared with SO rats. Moreover, in SAD rats, mean arterial pressure increased significantly (P < 0.05) while heart rate did not change during the freezing behavior. These data indicate that freezing behavior evokes regionally different changes in sympathetic outflows, which may be involved in generating the patterned responses of cardiovascular function to stressful or threatening sensory stimulation. Moreover, it is suggested that the arterial baroreceptors are involved in generating the differential changes in RSNA and LSNA and thus the patterned changes in cardiovascular functions observed during freezing behavior in conscious rats., Oct. 2010, 299, 4, R1114-20, Scientific journal, True, 10.1152/ajpregu.00831.2009

Refereed, Hypertension (Dallas, Tex. : 1979), Chronic angiotensin II infusion causes differential responses in regional sympathetic nerve activity in rats., Misa Yoshimoto; Kenju Miki; Gregory D Fink; Andrew King; John W Osborn, Angiotensin II (AngII)-induced hypertension in experimental animals has been proposed to be attributed in part to activation of the sympathetic nervous system. This sympathetic activation appears to be accentuated in animals consuming a high-salt diet (AngII-salt hypertension). However, accurate quantification of sympathetic activity is difficult, and controversy remains. It is particularly important to ask which are the critical vascular beds targeted by increased sympathetic nerve activity (SNA) in AngII-salt hypertension. To address this issue, mean arterial pressure and renal SNA or lumbar SNA were continuously recorded during a 5-day control period, 11 days of AngII (150 ng/kg per minute, SC), and a 5-day recovery period in conscious rats on a high-salt (2% NaCl) diet. Although mean arterial pressure reached a new steady-state level of 30 to 35 mm Hg above control levels by the end of the AngII period, renal SNA decreased by 40% during the first 7 days of AngII and then returned toward control levels by day 10 of AngII. In contrast, lumbar SNA remained at control levels throughout the AngII period. In another experiment we measured hindlimb norepinephrine spillover in conscious rats on normal (0.4%) or high- (2.0%) salt diets before and during 14 days of AngII administration. AngII had no significant affect on hindlimb norepinephrine spillover in either group. We conclude that chronic AngII modulates renal and lumbar SNAs differentially in rats consuming a high-salt diet and that AngII-salt hypertension in the rat is not caused by increased SNA to the renal or hindlimb vascular beds., Mar. 2010, 55, 3, 644, 51, Scientific journal, True, 10.1161/HYPERTENSIONAHA.109.145110

Refereed, Experimental physiology, Role of differential changes in sympathetic nerve activity in the preparatory adjustments of cardiovascular functions during freezing behaviour in rats., Kenju Miki; Misa Yoshimoto, Freezing behaviour is associated with a distinct pattern of changes in cardiovascular function, which has been considered as a preparatory reflex for 'fight or flight' behaviour. However, the detailed mechanisms underlying preparatory cardiovascular adjustments and their physiological implications have received less attention. We studied responses in renal and lumbar sympathetic nerve activity and cardiovascular function during freezing behaviour in conscious rats, which was induced by exposure to loud white noise. Freezing behaviour was associated with regionally specific alterations in sympathetic nerve activity, in that renal sympathetic nerve activity increased while lumbar sympathetic nerve activity did not change. Moreover, freezing behaviour was associated with differential shifts in baroreflex control of sympathetic outflows, which could help to explain the selective responses in renal and lumbar sympathetic nerve activity during freezing behaviour. These differential changes in sympathetic outflows would result in a visceral vasoconstriction without having any impact on the skeletal muscle vasculature. These cardiovascular adjustments during freezing behaviour may help to explain the immediate and massive increase in muscular blood flow that occurs at the onset of fight or flight behaviour. It is hypothesized that central command originating from the defence area could somehow modulate separate baroreflex pathways, causing differential changes in sympathetic nerve activity to generate the preparatory cardiovascular adjustments during the freezing behaviour., Jan. 2010, 95, 1, 56, 60, Scientific journal, True, 10.1113/expphysiol.2009.050187

Refereed, American journal of physiology. Regulatory, integrative and comparative physiology, Role of cardiopulmonary and carotid sinus baroreceptors in regulating renal sympathetic nerve activity during water immersion in conscious dogs., Kenju Miki; Misa Yoshimoto; Yoshiaki Hayashida; Keizo Shiraki, The aim of the present study was to investigate how loading of cardiopulmonary baroreceptors induced by water immersion (WI) modifies baroreflex control of renal sympathetic nerve activity (RSNA) in conscious dogs. Nine dogs were chronically instrumented for measuring carotid sinus nerve activity (CSNA), RSNA, carotid arterial (Pca), and central venous (Pcv) pressures. The stimulus-response relationships of Pca-CSNA and Pca-RSNA were determined simultaneously in the same dog by changing Pca using rapid intravenous infusions vasoactive drugs during pre-WI and WI. WI increased central venous pressure significantly (P < 0.05) by 10.4 mmHg. WI shifted the Pca-RSNA curve acutely leftward compared with the pre-WI period, which was associated with significant (P < 0.05) decreases in the saturation pressure by 39.0 mmHg and operating range by 43.1 mmHg. WI relocated the operating pressure to near the saturation pressure, where the gain was low. The Pca-CSNA curve obtained during WI was identical to that obtained during pre-WI period. These results suggest that the shift in baroreflex control of RSNA could be attributed to the inhibitory influence of the cardiopulmonary mechanoreceptor loading and not by the resetting of carotid baroreceptors per se., Jun. 2009, 296, 6, R1807-12, Scientific journal, True, 10.1152/ajpregu.00082.2008

Refereed, American journal of physiology. Heart and circulatory physiology, Effect of stellate ganglionectomy on basal cardiovascular function and responses to beta1-adrenoceptor blockade in the rat., Misa Yoshimoto; Erica A Wehrwein; Martin Novotny; Greg M Swain; David L Kreulen; John W Osborn, Cardiac sympathetic nerve activity is an important short-term controller of cardiac function and arterial pressure. Studies also suggest that long-term increases in cardiac sympathetic nerve activity may contribute to hypertension, coronary artery disease, and cardiac remodeling in heart failure. However, our understanding of the role of cardiac sympathetic nerves in chronic models of cardiovascular disease has been limited by inadequate experimental approaches. The present study was conducted to develop a surgical method to surgically denervate the sympathetic nerves of the rat heart for long-term cardiovascular studies. We characterized the effect of cardiac sympathetic denervation on basal levels of mean arterial pressure (MAP) and heart rate (HR) and the responses to a chronic administration of atenolol, a beta1-adrenoceptor antagonist. Rats were instrumented with telemetry transmitters for continuous recording of MAP and HR. After a 4-day baseline period, the rats were subjected to bilateral stellate ganglionectomy (SGX; n=9) or sham surgery (Sham; n=8). Seven days following SGX or Sham, the rats were administered atenolol for 5 days, followed by a 7-day recovery period. Following a transient decrease, SGX had no effect on basal MAP but decreased HR compared with baseline and Sham rats. Five days of atenolol treatment decreased MAP similarly in SGX and Sham rats. Atenolol resulted in a marked bradycardia in Sham rats but had a neglible effects on HR in SGX rats. The measurement of the content of cardiac catecholamines in all cardiac chambers at the end of the study verified a successful sympathetic denervation. This study confirms that bilateral SGX is a useful method to study the contribution of cardiac sympathetic nerves on the regulation of cardiac function. Moreover, these results suggest that cardiac sympathetic nerves are relatively unimportant in maintaining the basal level of MAP or the depressor response to atenolol in conscious, unrestrained rats., Dec. 2008, 295, 6, H2447-54, True, 10.1152/ajpheart.00958.2008

Refereed, The Journal of physiology, The contribution of brain angiotensin II to the baroreflex regulation of renal sympathetic nerve activity in conscious normotensive and hypertensive rats., Chunlong Huang; Misa Yoshimoto; Kenju Miki; Edward J Johns, Angiotensin II receptor density in the brain is elevated when dietary salt intake is raised or in the state of hypertension. The aim of this study was to evaluate whether the angiotensin II modulation of the baroreceptor control of renal sympathetic nerve activity was altered under these conditions. Wistar rats, fed either a regular (0.25% w/w sodium) or high-salt diet (3.1% w/w sodium), or stroke-prone spontaneously hypertensive rats (SHRSPs) were implanted with cannulae in the carotid artery, jugular vein and the cerebroventricle and with recording electrodes on the renal sympathetic nerves. Three days later, baroreceptor gain curves were generated for renal sympathetic nerve activity and heart rate before and following intracerebroventricular (i.c.v.) administration of losartan (15 mug) to block angiotensin AT1 receptors. The rats fed a regular diet had a mean blood pressure of 116 +/- 3 mmHg and heart rate of 467 +/- 25 beats min(-1), which remained unchanged after the i.c.v. administration of losartan. The sensitivity or curvature coefficient of the baroreceptor curve for renal sympathetic nerve activity was increased by 36% (P < 0.05) following losartan. In the rats fed a high-salt diet, all cardiovascular variables and the losartan-induced increase in the baroreceptor curvature coefficient for renal sympathetic nerve activity (29%) were similar to values in rats on the regular sodium diet. The heart rate baroreceptor curvature coefficient was not altered in either the rats fed a regular or a high-salt diet. The slope of the renal sympathetic nerve activity baroreflex gain curve in the SHRSPs was less and the increase following administration of losartan (54%) was greater than in the Wistar rats. These data indicate that in the conscious state, the tonic inhibitory action of brain angiotensin II on the baroreflex regulation of renal sympathetic nerve activity was unaffected by raised dietary sodium, but its role was enhanced in the SHRSPs., 15 Jul. 2006, 574, Pt 2, 597, 604, Scientific journal, True

Refereed, Experimental physiology, Differential effects of behaviour on sympathetic outflow during sleep and exercise., Kenju Miki; Misa Yoshimoto, The responses of renal and lumbar sympathetic outflow to changes in behavioural states were reviewed in this paper. During rapid eye movement (REM) sleep, renal sympathetic nerve activity was decreased while lumbar sympathetic nerve activity increased. These diverse changes in sympathetic nerve activity observed during REM sleep help explain the responses in regional blood flow to REM sleep; that is renal blood flow increased while muscle blood flow decreased. By contrast, exercise increased both renal and muscle sympathetic nerve activity. The degree of physical activity was correlated with the magnitude of the increases in renal and muscle sympathetic nerve activity. There was a significant (P<0.05) linear relationship between renal sympathetic nerve activity and systemic arterial pressure over the transition between non-rapid eye movement (NREM) sleep, quiet awake, moving and grooming states in the rats. This suggests that sympathetic outflows seem to be modulated quantitatively to meet cardiovascular demand caused by changes in the level of physical activity. It is therefore concluded that sympathetic outflow seems to be regulated in a state-specific manner during sleep and exercise., Mar. 2005, 90, 2, 155, 8, Scientific journal, True

Refereed, Journal of applied physiology (Bethesda, Md. : 1985), Regulation of gastric motility at simulated high altitude in conscious rats., Misa Yoshimoto; Mitsuko Sasaki; Nobuo Naraki; Motohiko Mohri; Kenju Miki, The aim of the present study was to examine the effects of acute exposure to hypobaric hypoxia on gastric and colonic motilities. Wistar rats, which were instrumented chronically with strain gauge force transducer to measure gastric and colonic motilities, were exposed acutely to hypobaric hypoxia [0.5 atmosphere absolute (ATA, 380 Torr)] over 1 h. In a separate group, the gastric branches of the vagal nerves were cut and underwent the same experimental protocol. Each contraction wave of the stomach and colon was analyzed into frequency and area under the curves, which were then averaged every 10 min. Acute exposure to 0.5 ATA resulted in significant (P < 0.05) decreases in frequency and area of gastric contraction wave by 0.5 +/- 0.1 cycles/min and 64.6 +/- 4.0%, respectively. Gastric vagotomy abolished completely the suppression in the area observed in the intact rats during the 0.5-ATA exposures. Colonic motility increased significantly only at the start and end of exposure to 0.5 ATA and sham exposure [1 ATA (760 Torr), time control] in both intact and vagotomized rats. These data suggest that the acute suppression of the area of the gastric contraction wave that occurred during 0.5-ATA exposure is likely to be mediated by the vagal nerve., Aug. 2004, 97, 2, 599, 604, True

Refereed, The Journal of physiology, Acute shifts in baroreflex control of renal sympathetic nerve activity induced by REM sleep and grooming in rats., Satsuki Nagura; Tamaki Sakagami; Ai Kakiichi; Misa Yoshimoto; Kenju Miki, The present study aimed to determine the impact of REM sleep and grooming on the baroreflex stimulus-response curve for renal sympathetic nerve activity (RSNA). At least 3 days before study, Wistar female rats (n= 12) were chronically implanted with catheters to measure systemic arterial pressure (P(a)) and to intravenously infuse vasoactive drugs. In addition, electrodes were placed for measurements of RSNA, electroencephalogram, trapezius electromyogram and electrocardiogram. The baroreflex curve for RSNA was determined by changing P(a) using rapid intravenous infusions of phenylephrine and nitroprusside and then fitted to an inverse sigmoid function curve. REM sleep induced a vertical suppression of the P(a)-RSNA baroreflex curve, which was characterized by significant decreases in the maximum response (by 72.0%, P < 0.05) and the maximum gain (by 4.02% mmHg(-1), P < 0.05) compared with NREM sleep level. Grooming shifted the P(a)-RSNA baroreflex curve upward and to the right, which was associated with increases in the maximum response (by 45.2%, P < 0.05), the minimum response (by 20.7%, P < 0.05) and the pressure at the centering point (by 11.1 mmHg, P < 0.05). These data suggest that the P(a)-RSNA baroreflex curve was shifted acutely and differently in a state-dependent manner during natural sleep and wake cycle in rats., 01 Aug. 2004, 558, Pt 3, 975, 83, Scientific journal, True

Refereed, The Journal of physiology, Lumbar sympathetic nerve activity and hindquarter blood flow during REM sleep in rats., Kenju Miki; Michiyo Oda; Nozomi Kamijyo; Kazumi Kawahara; Misa Yoshimoto, The present study aimed to investigate the response of lumbar sympathetic nerve activity (LSNA) to the onset of rapid eye movement (REM) sleep and its contribution to the regulation of muscle blood flow during REM sleep in rats. Electrodes for the measurements of LSNA, electroencephalogram, electromyogram and electrocardiogram and a Doppler flow cuff for the measurements of blood flow in the common iliac and mesenteric arteries, also catheters for the measurements of systemic arterial and central venous pressures were implanted chronically. REM sleep resulted in a step increase in LSNA, by 22 +/- 9% (mean +/-S.E.M., P < 0.05), a reduction of iliac vascular conductance, by -16 +/- 3% (P < 0.05) and a gradual increase in systemic arterial pressure, reaching a maximum value of 8.1 +/- 2.0 mmHg (P < 0.05) at 89 s after onset of REM sleep, while mesenteric vascular conductance increased simultaneously by 5 +/- 2% (P < 0.05). There was a significant (Pearson's correlation coefficient = 0.94, P < 0.05) inverse linear relationship between LSNA and the iliac blood flow. Unilateral lumbar sympathectomy blunted the reduction of iliac blood flow induced by the onset of REM sleep. The present observations suggest that the onset of REM sleep appears to be associated with a vasodilation in viscera and a vasoconstriction in skeletal muscle, such that systemic arterial pressure increases during REM sleep in rats., 15 May 2004, 557, Pt 1, 261, 71, Scientific journal, True

Refereed, American journal of physiology. Regulatory, integrative and comparative physiology, Relationship between renal sympathetic nerve activity and renal blood flow during natural behavior in rats., Misa Yoshimoto; Tamaki Sakagami; Satsuki Nagura; Kenju Miki, The purpose of the present study was to determine the relationship between renal sympathetic nerve activity (RSNA) and renal blood flow (RBF) during normal daily activity in conscious, chronically instrumented Wistar rats (n = 8). The animal's behavior was classified as rapid eye movement (REM) sleep, non-REM (NREM) sleep, quiet awake, moving, and grooming states. On average RSNA was lowest during REM sleep, which was decreased by 39.0 +/- 3.2% (P < 0.05) relative to NREM sleep, and rose linearly with an increase in activity level in the order of quiet awake (by 10.9 +/- 1.8%, P < 0.05), moving (by 29.4 +/- 2.9%, P < 0.05), and grooming (by 65.3 +/- 3.9%, P < 0.05) relative to NREM sleep. By contrast, RBF was highest during REM sleep, which was increased by 4.8 +/- 0.7% (P < 0.05) relative to NREM sleep and decreased significantly (P < 0.05) by 5.5 +/- 0.6 and 6.6 +/- 0.5% during moving and grooming states, respectively, relative to NREM sleep. There was a significant (P < 0.05) inverse linear relationship between the percent changes in RSNA and RBF and between those in RSNA and renal vascular conductance. Furthermore, renal denervation (n = 8) abolished the changes in RBF induced by different natural behavioral activities. These results suggest that the changes in RSNA induced by natural behavioral activities had a significant influence on RBF., May 2004, 286, 5, R881-7, Scientific journal, True

Refereed, The Journal of physiology, Acute shifts of baroreflex control of renal sympathetic nerve activity induced by treadmill exercise in rats., Kenju Miki; Misa Yoshimoto; Momoko Tanimizu, The present study aimed to investigate whether there was a resetting of the baroreflex control of renal sympathetic nerve activity (RSNA) and heart rate (HR) during exercise. Wistar female rats (n = 11) were chronically implanted with catheters for the measurement of systemic arterial (Pa) and central venous pressures and with electrodes for measurement of RSNA and electrocardiogram (ECG) at least 3 days before study. The baroreflex curve for RSNA was determined by changing Pa using rapid intravenous infusions of phenylephrine and nitroprusside. The baroreflex response curves for RSNA and HR were characterized by an inverse sigmoid function curve from which the response range, gain, centering point and minimum response were estimated. Exercise shifted the Pa-RSNA baroreflex curve upward and to the right and was associated with increases in response range of 122 +/- 44 % (P < 0.05), maximum response of 173 +/- 40 % (P < 0.05), maximum gain of 149 +/- 66 % (P < 0.05) and midpoint pressure of 15 +/- 5 mmHg (P < 0.05) compared with the pre-exercise level. After cessation of exercise, the Pa-RSNA baroreflex curve was suppressed vertically with a significant decrease in maximum response of 57 +/- 14 % (P < 0.05) compared with the pre-exercise level. These data suggest that the right-upward shift of baroreflex control of sympathetic nerve activity may play a critical role in raising and stabilizing Pa during exercise. The suppression of the baroreflex control of sympathetic nerve activity may partly explain the post-exercise inhibition of sympathetic nerve activity and contribute to the post-exercise hypotension., 01 Apr. 2003, 548, Pt 1, 313, 22, Scientific journal, True

Frontiers in Physiology, Frontiers Media SA, Regional Differences in Sympathetic Nerve Activity Are Generated by Multiple Arterial Baroreflex Loops Arranged in Parallel, Kenju Miki; Shizuka Ikegame; Misa Yoshimoto, In this review, by evaluating the responses during freezing, rapid eye movement (REM) sleep, and treadmill exercise, we discuss how multiple baroreflex loops arranged in parallel act on different organs to modulate sympathetic nerve activity (SNA) in a region-specific and coordinated manner throughout the body. During freezing behaviors, arterial pressure (AP) remains unchanged, heart rate (HR) persistently decreases, renal SNA (RSNA) increases, and lumbar SNA (LSNA) remains unchanged. The baroreflex curve for RSNA shifts upward; that for LSNA remains unchanged; and that for HR shifts to the left. These region-specific changes in baroreflex curves are responsible for the region-specific changes in RSNA, LSNA, and HR during freezing. The decreased HR could allow the heart to conserve energy, which is offset by the increased RSNA caused by decreased vascular conductance, resulting in an unchanged AP. In contrast, the unchanged LSNA leaves the muscles in readiness for fight or flight. During REM sleep, AP increases, RSNA and HR decrease, while LSNA is elevated. The baroreflex curve for RSNA during REM sleep is vertically compressed in comparison with that during non-REM sleep. Cerebral blood flow is elevated while cardiac output is decreased during REM sleep. To address this situation, the brain activates the LSNA selectively, causing muscle vasoconstriction, which overcomes vasodilation of the kidneys as a result of the decreased RSNA and cardiac output. Accordingly, AP can be maintained during REM sleep. During treadmill exercise, AP, HR, and RSNA increase simultaneously. The baroreflex curve for RSNA shifts right-upward with the increased feedback gain, allowing maintenance of a stable AP with significant fluctuations in the vascular conductance of working muscles. Thus, the central nervous system may employ behavior-specific scenarios for modulating baroreflex loops for differential control of SNA, changing the SNA in a region-specific and coordinated manner, and then optimizing circulatory regulation corresponding to different behaviors., 04 Apr. 2022, 13, 858654, 858654, True, 10.3389/fphys.2022.858654

Refereed, 72-72, 2021., 運動による交感神経活動と動脈圧調節の変化, 吉本光佐; 三木健寿, Dec. 2021, suppl, 58, 72, 72, Introduction scientific journal, 10.32272/ans.59.3_293

The FASEB Journal, Wiley, Effects of Social Defeat on Hippocampal CA1 Neuronal and Sympathetic Nerve Activities in Conscious Rats, Kana Yaguchi; Shizuka Ikegame; Misa Yoshimoto; Kenju Miki, Apr. 2020, 34, S1, 1, 1, Summary international conference, 10.1096/fasebj.2020.34.s1.05015

The FASEB Journal, Wiley, Simultaneous Measurement of Hypothalamic Paraventricular Nucleus Neuronal and Sympathetic Nerve Activity in Freely Moving Rats, Shizuka Ikegame; Misa Yoshimoto; Kenju Miki, Apr. 2019, 33, S1, 10.1096/fasebj.2019.33.1_supplement.744.3

The FASEB Journal, Wiley, Differential Renal and Lumbar Sympathetic Nerve Activation Following Myocardial Infarction in Rats, Misa Yoshimoto; Shizuka Ikegame; Fumi Hyodo; Yuki Shiwa; Kenju Miki, Apr. 2019, 33, S1, 10.1096/fasebj.2019.33.1_supplement.690.7

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Method for Simultaneous Measurement of Amygdala Neuronal and Sympathetic Nerve Activity in Freely Moving Rats, Kataoka Shizuka; Misa Yoshimoto; Fukiko Ootsuki; Kenju Miki, Apr. 2017, 31, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Hippocampal CA1 Neuronal and Sympathetic Nerve Activity in Response to Repeated Fear Conditioning in Rats, Hiroka Ukita; Misa Yoshimoto; Kana Nagao; Kenju Miki, Apr. 2017, 31, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Sympathetic Nerve Activity during Development of Deoxycorticosterone Acetate-Salt Hypertension in Rat, Misa Yoshimoto; Yuki Shiwa; Shiori Setoguchi; Shiho Kawamura; Kenju Miki, Apr. 2017, 31, Summary international conference

Not Refereed, CIRCULATION CONTROL, Japan Society of Circulation Control in Medicine, Benefits of cardiac microdialysis technique in the cardiovascular research, 秋山 剛; 清水 秀二; 杜 成坤; 戦 冬雲; 稲垣 董克; 吉本 光佐; 曽野部 崇; 川田 徹; ピアソン ジェームズ, We have applied the microdialysis technique used in neurochemical studies of the brain to the heart. Cardiac microdialysis technique enables us to directly monitor norepinephrine released from cardiac sympathetic nerve endings as well as acetylcholine released from cardiac vagal nerve endings and provides many advantages in the assessment of cardiac autonomic nerve activities総説 217and nerve ending functions including reuptake. Moreover, the cardiac microdialysis technique makes it possible to monitor myocardial interstitial concentrations of endogenous substances in the ischemic region continuously during ischemia as well as after reperfusion and locally administer pharmacological agents to the ischemic region through dialysis probes. The cardiac microdialysis technique is a powerful method for the study of pathophysiology during myocardial ischemia- reperfusion., 2017, 38, 3, 208, 217

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Role of the peripheral chemoreceptor reflex on cardiovascular and sympathetic responses to obstructive sleep apnea in rats, Kenju Miki; Misa Yoshimoto; Chisato Kajihara; Yuri Mizukami, Apr. 2015, 29, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Sympathetic nerve activity and arterial pressure during development of obesity in Zucker fatty rats, Yuki Shiwa; Misa Yoshimoto; Kenju Miki; Rika Okano, Apr. 2015, 29, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Relationship between hippocampal CA1 neuronal and sympathetic nerve activity during fear conditioning in rats, Misa Yoshimoto; Misaki Kanayama; Kenju Miki, Apr. 2015, 29, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Estradiol replacement attenuates stress-induced pressor response by regulating renin-angiotensin system in ovariectomized rats, Yu Nagatomo; Shoko Tazumi; Risa Itoh; Misa Yoshimoto; Akira Takamata; Keiko Morimoto, Apr. 2015, 29, Summary international conference

Not Refereed, JOURNAL OF PHYSIOLOGICAL SCIENCES, SPRINGER JAPAN KK, Changes in renal sympathetic nerve activity during development of renovascular-hypertensive rats, Misa Yoshimoto; Tsuyoshi Akiyama; Takashi Sonobe; Yutaka Fujii; Kenju Miki; Mikiyasu Shirai, 2013, 63, S269, S269, Summary international conference

Not Refereed, JOURNAL OF PHYSIOLOGICAL SCIENCES, SPRINGER JAPAN KK, Abnormal cross-bridge dynamics in the in situ beating rat heart in early diabetes, Mikiyasu Shirai; Mathew Jenkins; Takashi Sonobe; Yutaka Fujii; Hirotsugu Tsuchimochi; Misa Yoshimoto; Tadakatsu Inagaki; Naoto Yagi; James Pearson, 2013, 63, S182, S182, Summary international conference

Not Refereed, JOURNAL OF PHYSIOLOGICAL SCIENCES, SPRINGER JAPAN KK, Time course of changes in sympathetic nerve activity and heart rate during development of hypertension in spontaneously hypertensive rats, Naoko Mineyama; Misa Yoshimoto; Hiroko Awazu; Yumi Onishi; Asuka Shimazu; Mikiyasu Shirai; Kenju Miki, 2013, 63, S179, S179, Summary international conference

日本生気象学会雑誌, 寒冷及び暑熟環境が睡眠時の循環調節に及ぼす影響, 佐藤 邑子; 助口 知絵; 吉本 光佐; 三木 健寿, 01 Oct. 2012, 49, 3, S53

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Renal and lumber sympathetic nerve activity during the development of hypertension in Dahl-Salt-Sensitive rats, Misa Yoshimoto; Yuko Onishi; Naoko Mineyama; Mikiyasu Shirai; Kenju Miki, Apr. 2012, 26, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Progressive increase in renal sympathetic nerve activity in cold-induced hypertension in rats, Kenju Miki; Kana Yagi; Misa Yoshimoto, Apr. 2012, 26, Summary international conference

Not Refereed, 日本内分泌学会雑誌, (一社)日本内分泌学会, モノクロタリン肺高血圧ラットの肺微小循環調節におけるRho-kinaseシグナル及び内皮機能の役割, 白井 幹康; シュエンキ・ダリル; 曽野部 崇; 藤井 豊; 吉本 光佐; 寒川 賢治; 梅谷 啓二, Sep. 2011, 87, 2, 784, 784

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Long-term responses of renal and lumbar sympathetic nerve activity to chronic sino-aortic denervation in rats, Kenju Miki; Misa Yoshimoto; Mikiyasu Shirai; Yuko Onishi; Kana Yagi, Apr. 2011, 25, Summary international conference

Not Refereed, NEUROSCIENCE RESEARCH, ELSEVIER IRELAND LTD, Chronic responses of renal and lumbar sympathetic nerve activity and arterial pressure to sino-aortic baroreceptor denervation in rats, Yuko Onishi; Misa Yoshimoto; Kana Yagi; Mikiyasu Shirai; Kenju Miki, 2011, 71, E161, E161, Summary international conference, 10.1016/j.neures.2011.07.695

Refereed, 循環器病研究の進歩, SPring-8高輝度放射光を用いた小動物の心臓・血管機能の画像解析, 白井 幹康; Pearson James; Schwenke Daryl; 曽野部 崇; 藤井 豊; 吉本 光佐; 徳留 健; 清水 壽一郎; 寒川 賢治; 梅谷 啓二; 八木 直人, Nov. 2010, XXXI, 1, 68, 80

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Diverse Changes in Sympathetic Nerve Activity during REM Sleep in Rats, Kenju Miki; Misa Yoshimoto, Apr. 2010, 24, Summary international conference

Not Refereed, NEUROSCIENCE RESEARCH, ELSEVIER IRELAND LTD, Long-term responses of renal sympathetic nerve activity and arterial pressure to chronic sino-aortic denervation in rats, Yuko Onishi; Kana Yagi; Misa Yoshimoto; Kenju Miki, 2010, 68, E169, E169, Summary international conference, 10.1016/j.neures.2010.07.2324

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Relationship between hippocampal neuron activity and regional cerebral blood flow during sleep-wake cycle in rats, Momoka Tanji; Misa Yoshimoto; Kenju Miki, Apr. 2009, 23, Summary international conference

Not Refereed, JOURNAL OF PHYSIOLOGICAL SCIENCES, SPRINGER TOKYO, NEUROVASCULAR COUPLING IN THE HIPPOCAMPUS IS ALTERED DURING REM SLEEP IN RATS, Momoka Tanji; Misa Yoshimoto; Kenju Miki, 2009, 59, 521, 521, Summary international conference

Not Refereed, HYPERTENSION, LIPPINCOTT WILLIAMS & WILKINS, Differential responses of renal and muscle sympathetic nerve activity to chronic angiotensin II administration in rats consuming a high-salt diet, Misa Yoshimoto; Kenju Miki; Andrew King; Gregory Fink; John W. Osborn, Oct. 2008, 52, 4, E64, E64, Summary international conference

Not Refereed, HYPERTENSION, LIPPINCOTT WILLIAMS & WILKINS, Regional hind-limb (HL) hemodynamics and norepinephrine (NE) spillover in chronic angiotensin II (AngII) - Salt hypertension in the rat, Andrew J. King; Misa Yoshimoto; John W. Osborn; Gregory D. Fink, Oct. 2008, 52, 4, E64, E64, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Effect of stellate ganglionectomy (SGx) on the long-term responses of arterial pressure (AP) and heart rate (HR) to beta-1 adrenergic blockade in conscious rats, Misa Yoshimoto; Martin Novotny; Dasa Babankova; Gregory M. Swain; Erica A. Wehrwein; David Kreulen; John Osborn, Apr. 2008, 22, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Continuous long-term recording of renal (RSNA) and lumber (LSNA) sympathetic nerve activity and arterial pressure (AP) in conscious rats: Responses to chronic angiotensin II (AngII) administration, Misa Yoshimoto; Kenju Miki; John W. Osborn, Apr. 2008, 22, Summary international conference

Not Refereed, NEUROSCIENCE RESEARCH, ELSEVIER IRELAND LTD, Hippocampal CA1 neuron activity during sleep-wake cycle in rats, Momoka Tanji; Misa Yoshimoto; Kenju Miki, 2008, 61, S254, S254, Summary international conference

日本平滑筋学会雑誌, 日本平滑筋学会, S-19. 迷走神経が低気圧環境での胃運動調節に果たす役割(自律神経支配臓器の神経性機能調節, 第49回日本平滑筋学会総会), 三木 健寿; 吉本 光佐; 楢木 暢雄; 毛利 元彦, 26 Jun. 2007, 11, 1, "J, 15"

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Differential response of renal and lumbar sympathetic nerve activity during freezing behaviour in conscious rats, Misa Yoshimoto; Kenju Miki, Apr. 2007, 21, 6, A882, A882, Summary international conference

Not Refereed, NEUROSCIENCE RESEARCH, ELSEVIER IRELAND LTD, Local cerebral blood flow in the hippocampus during REM sleep in rats, Kenju Miki; Tomoko Hata; Junko Abe; Misa Yoshimoto, 2007, 58, S167, S167, Summary international conference

日本生気象学会雑誌, オレキシン神経がマウスの自律神経機能に及ぼす影響, 吉本 光佐; 三木 健寿, 01 Oct. 2006, 43, 3, S65

日本生気象学会雑誌, ニコチンがラットの脳波および海馬血流量に及ぼす影響, 近藤 直美; 芝崎 学; 吉本 光佐; 三木 健寿, 01 Oct. 2006, 43, 3, S40

Not Refereed, NEUROSCIENCE RESEARCH, ELSEVIER IRELAND LTD, Differential control of renal and lumbar sympathetic nerve activity during freezing behaviour in conscious rats, Yoshimi Tahara; Misa Yoshimoto; Keiko Nagata; Kenju Miki, 2006, 55, S128, S128, Summary international conference

Not Refereed, NEUROSCIENCE RESEARCH, ELSEVIER IRELAND LTD, Responses of renal sympathetic nerve activity and sodium excretion to 3 days sodium loading in rats, Misa Yoshimoto; Nozomi Iinuma; Rie Itokawa; Eri Hayashi; Kenju Miki, 2006, 55, S57, S57, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Method for continuous monitoring of renal sympathetic nerve activity in freely moving mice, M Yoshimoto; K Miki, Mar. 2005, 19, 4, A604, A604, Summary international conference

Not Refereed, FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Impact of renal sympathetic nerve activity on the regulation of renal blood flow during daily activity in rats, K Miki; M Yoshimoto, Mar. 2003, 17, 4, A113, A113, Summary international conference

Not Refereed, JOURNAL OF PHYSIOLOGY-LONDON, CAMBRIDGE UNIV PRESS, Sympathetic regulation of renal blood flow during natural behaviour in rats, K Miki; T Sakagami; S Nagura; M Yoshimoto, Feb. 2002, 539, 132P, 133P, Summary international conference

The FASEB Journal, Wiley, Method for Measuring Cervical Vagus Nerve Activity in Conscious and Freely Moving Rats, Kenju Miki; Shizuka Ikegame; Misa Yoshimoto, 13 May 2022, 36, S1, 10.1096/fasebj.2022.36.s1.r4360

日本生理学雑誌(Web), 意識下ラットでの頸部迷走神経活動計測の試み, 宮浦和奏; 三木健寿; 池亀静香; 吉本光佐, 2022, 84, 1

日本生理学雑誌(Web), バゾプレッシン神経の選択的刺激による腎交感神経活動の変化, 高井香里; 武井珠里; 宮浦和奏; 森本夏実; 池亀静香; 丸山崇; 上田陽一; 三木健寿; 吉本光佐, 2022, 84, 1

日本自律神経学会総会プログラム・抄録集, 迷走神経活動の計測, 三木健寿; 吉本光佐, 2022, 75th

浦上財団研究報告書, Sympathetic nerve activity as an index for seeking anti-obesity meal patterns., 吉本光佐, 2020, 27

日本生気象学会雑誌, 閉塞性睡眠時無呼吸が海馬領域血流量と海馬CA1神経活動に与える影響と交感神経の関与, 矢口佳奈; 三木健寿; 吉本光佐, 2020, 57, 3

日本生理学雑誌(Web), 匂いによる恐怖記憶想起時の交感神経活動と循環動態の変化, 矢口佳奈; 池亀静香; 三木健寿; 吉本光佐, 2020, 82, 1

日本生理学雑誌(Web), 拘束浸水ストレスがラットの海馬組織血流量調節に及ぼす影響, 増田柚果; 矢口佳奈; 池亀静香; 三木健寿; 吉本光佐, 2020, 82, 1

日本生気象学会雑誌, 睡眠時の視床下部室傍核神経活動と交感神経活動の周波数分析, 池亀静香; 吉本光佐; 三木健寿, 2019, 56, 3

日本生気象学会雑誌, 意識下自由行動下ラットにおける視床下部室傍核神経活動と交感神経活動と同時測定の試み, 池亀静香; 吉本光佐; 三木健寿, 2018, 55, 3

日本生気象学会雑誌, 恐怖条件付けされたにおいに対する扁桃体神経活動と循環動態の変化, 片岡静香; 吉本光佐; 三木健寿, 2017, 54, 3

日本生気象学会雑誌, 拘束浸水ストレス負荷によるラットの海馬CA1神経活動,腎及び腰部交感神経活動と循環動態の変化, 浮田紘香; 吉本光佐; 三木健寿, 2017, 54, 3

日本生理学雑誌(Web), 肺伸展受容器が閉塞性睡眠時無呼吸時の循環調節に果たす役割, 片岡静香; 吉本光佐; 中村仁美; 三木健寿, 2016, 78, 1

日本生理学雑誌, 脳卒中易発症高血圧自然発症ラットにおいて交感神経活動が高血圧発症に果たす役割, 志波侑希; 吉本光佐; 岡野里香; 三木健寿, 2015, 77, 1 (Web)

日本生理学雑誌, 閉塞性睡眠時無呼吸時の循環調節に果たす末梢性化学受容器の役割, 梶原千聖; 吉本光佐; 水上友里; 三木健寿, 2015, 77, 1 (Web)

日本生気象学会雑誌, ラットにおける恐怖記憶想起にともなう交感神経活動の応答と海馬神経活動との相同性, 金山美沙貴; 吉本光佐; 梶原千聖; 三木健寿, 2014, 51, 3

日本生気象学会雑誌, ラットの海馬神経活動と腎及び腰部交感神経活動の同時測定とその相同性の検討, 齋藤理郁; 助口知絵; 吉本光佐; 三木健寿, 2013, 50, 3

日本生化学会大会(Web), 抗肥満作用を示すBMP-3b過剰発現マウスの機能解析及び脂肪細胞におけるBMP-3bの遺伝子発現調節, 日野純; 中谷直史; 荒井勇二; 宮澤崇; 吉本光佐; 白井幹康; 土田邦博; 宮里幹也; 寒川賢治, 2013, 86th

肥満研究, BMP-3bの新規アディポカインとしての機能:過剰発現マウスによる抗肥満作用および代謝解析, 日野純; 中谷直史; 荒井勇二; 宮澤崇; 吉本光佐; 白井幹康; 土田邦博; 宮里幹也; 寒川賢治, 2013, 19, Supplement

日本自律神経学会総会プログラム・抄録集, 高血圧発症に果たす交感神経活動の役割-動物実験による検証, 吉本光佐, 2012, 65th

日本平滑筋学会雑誌, 迷走神経が低気圧環境での胃運動調節に果たす役割, 三木健寿; 吉本光佐; 楢木暢雄; 毛利元彦, 2007, 11, 1

宇宙利用シンポジウム, 交感神経活動の微小重力環境への長期適応過程と体液調節, 三木健寿; 森本恵子; 鷹股亮; 芝崎学; 吉本光佐, 2006, 22nd

日本生気象学会雑誌, 環境温度と行動の変化が交感神経活動に及ぼす影響, 吉本光佐; 三木健寿, 2005, 42, 3

日本神経科学大会プログラム・抄録集, ラット睡眠レム期における交感神経の地域差と組織血流量調節, 吉本光佐; 小田理代; 吉田郁恵; 三木健寿, 2002, 25th

日本自律神経学会総会プログラム・抄録集, 運動による交感神経活動と動脈圧調節の変化, 吉本光佐; 三木健寿, 2021, 74th

Oral presentation, 27 Mar. 2024, 27 Mar. 2024

Oral presentation, 11 Nov. 2023, 11 Nov. 2023

Poster presentation, 20 Apr. 2023, 23 Apr. 2023

Invited oral presentation, 27 Oct. 2022, 28 Oct. 2022

Oral presentation, 13 Mar. 2022, 13 Mar. 2022

Poster presentation, 02 Apr. 2022, 03 Apr. 2022

Keynote oral presentation, 28 Nov. 2020, 29 Nov. 2020

Public symposium, 28 Mar. 2021, 30 Mar. 2021

吉本 光佐, 第74回日本自律神経学会総会, 運動による交感神経活動と動脈圧調節の変化, Invited oral presentation, 24 Oct. 2021, 23 Oct. 2021

Japan society, 30 Mar. 2021

入澤宏・彩記念若手研究奨励賞日本生理学会奨励賞 [心臓・循環分野], 日本生理学会, 吉本光佐, Mar. 2012, 高食塩食とアンジオテンシン投与による高血圧発症時の交感神経活動の変化

久野寧記念賞, 日本生理学会環境生理グループ, Misa Yoshimoto, Mar. 2013, Functional Role of Diverse Changes in Sympathetic Nerve Activity in Regulating Arterial Pressure during REM Sleep

第44回日本生気象学会大会若手研究者発表コンテスト, 日本生気象学会, 吉本光佐, Oct. 2005

2020, 2025, JPMJMS2023-13, Coinvestigator

2023, 2025, 基盤研究(C), Principal investigator

2020, 2025, JPMJMS2023-13, Coinvestigator

基盤研究(C), 01 Apr. 2020, 31 Mar. 2023, 20K11623, 高血圧発症は若年期の食塩過剰摂取による交感神経性慢性炎症反応が原因か？, 吉本 光佐; 三木 健寿, 日本学術振興会, 科学研究費助成事業 基盤研究(C), 奈良女子大学, 4420000, 3400000, 1020000, 本研究では、若年期、特に離乳直後からの高食塩食摂取が成人期の動脈圧にどの様な影響を及ぼすか、また、この幼少期の高食塩負荷によって引き起こされる高血圧発症時の交感神経活動の動向を、実際に交感神経活動の直接計測で検討する事を目的としている。R2年度の検討より、離乳後の3週齢ラットに8%食塩食を摂取させると、12週齢で、通常食のコントロール群に比べて動脈圧は有意に高い値を示した。そこで、R3年度は、R2年度度と同様に、３週齢から4％食塩食を摂取させて、どの時点で動脈圧が上昇するかを検討した。 (方法)8週齢時に腎および腰部交感神経活動測定の為の電極と動脈圧と心拍数測定のためのテレメトリーを慢性留置した。9週齢で測定を開始し、その後４週間連続測定を行なった。さらに、手術開始時期を変えて動脈圧がいつ上昇するかの検討を行った。(結果)3週齢から４％高食塩食を摂取させたラットで８週齢に手術でした群では、9週齢時の動脈圧の平均値は、117.53±5.21mmHg、心拍数は396.17±19.85 beat/minsであり、12週齢時の動脈圧の平均は129.51±5.18、心拍数405.16±14.05beat/minsであった。また、11週齢に手術を開始した群では、12週齢時の動脈圧と心拍数の平均値は、118.92±4.9mmHg、351.71±20.8 beat/minsであり、15週齢時の動脈圧と心拍数の平均値は、117.88±6.8mmHg、340.71±15.2 beat/minsであった。いずれの時期においても４週間の連続測定の期間中に測定開始時に比べ動脈圧および心拍数の有意な変化はなかった。腎および腰部交感神経活動の変化に関しても計測期間で有意な変化は見られなかった。これらの結果をふまえ、今年度は手術開始期間を早める群とさらに遅らせる群を追加し、さらに長期間の検討を行う予定である。, kaken

Grant-in-Aid for Scientific Research (C), 01 Apr. 2017, 31 Mar. 2020, 17K00920, Is the risk of cardiovascular disease due to low sodium intake due to increased sympathetic nerve activity?, Yoshimoto Misa, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Nara Women's University, 4550000, 3500000, 1050000, To study the contribution of sympathetic nerve activity (SNA) to the process of low sodium intake, experiments were designed to continuously and simultaneously measure renal (RSNA) and lumbar SNA during the development of hypertension induced by 8% salt loading and then returned to a normal diet to low sodium diet in Dahl salt-sensitive rats. Mean arterial pressure (MAP) significantly increased during 2 weeks of salt loading. However, the sympathetic nerve activity did not increase. After to the process of low sodium intake, MAP returned to the pre-salt loading level, but RSNA was significantly increased by 32 ± 3%. It was suggested that RSNA activity is hypersensitive to salt loss., url;kaken

Grant-in-Aid for Young Scientists (B), 01 Apr. 2014, 31 Mar. 2017, 26860165, Is hypertension due to sleep apnea caused by hyperactivation of sympathetic nervous activity?, YOSHIMOTO MISA, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Nara Women's University, 4030000, 3100000, 930000, Sleep apnea syndrome (SAS) is complicated by cardiovascular diseases. Activation of sympathetic nerve activity has been suggested to contribute the cardiovascular diseases, while there has been little direct evidence on sympathetic nerve activity (SNA). We measured directly response of SNA to elucidate causal linkage between hypertension and activation of SNA in response to SAS. To simulate SAS in rats, Intermittent hypoxia (IH) and a balloon implanted in the trachea induction of apnea. Arterial pressure (AP) and renal SNA (RSNA) increased immediately after the onset of exposed 5% IH every time, but baselines did not change. While, AP and RSNA increased immediately after the onset of the obstruction and baseline of RSNA increase day bay day., url;kaken

Grant-in-Aid for Young Scientists (B), 2011, 2013, 23790285, To reveal a cause of development of hypertension with long term measurement of sympathetic nerve activity, YOSHIMOTO Misa, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), National Cardiovascular Center Research Institute, 4420000, 3400000, 1020000, Hypertension is a disease. One in four adults in the world have been diagnosed with high blood pressure. The aim of the present study was to explore a potential role of sympathetic nerve activity in the initial development of hypertension. The aim of the present study was to explore a potential role of sympathetic nerve activity in the initial development of hypertension. Salt sensitive hypersensitive rats and stroke-prone spontaneously hypertensive rats were used this experiments. The rats were chronically instrumented with a bipolar electrode for measurements of renal (RSNA) and lumbar sympathetic nerve activity (LSNA) and telemeter for measurements of arterial pressure (AP) and heart rate (HR). RSNA and LSNA apparently did not increase while development of hypertension occurred. It is therefor likely that possible contribution of sympathetic nerve activity to the initial development of hypertension in those hypersensitive rats remains uncertain., url;kaken

Dec. 2020, Nov. 2025, JPMJMS2023, Coinvestigator, 恒常性の理解と制御による糖尿病および併発疾患の克服, 吉本光佐, 国立研究開発法人科学技術振興機構, ムーンショット型研究開発事業, 奈良女子大学

JPMJMS2023-13, Coinvestigator