Sato-Nara Kumi

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Profile and Settings

• Name (Japanese)

  Kumi

• Name (Kana)

  Sato-Nara

Degree

• Doctor of Science, Tohoku University

Research Interests

• Water transport, Aquaporin (PIP1, PIP2, TIP1, TIP2), light regulation of plant development, NMR imaging (MRI)

Research Areas

• Life sciences, Plants: molecular biology and physiology

Research Experience

• Apr. 2012, Associate Professor, Division of Natural Sciences, Faculty, Nara Women's University
• Apr. 2007, Mar. 2012, Associate Professor, Faculty of Science, Nara Women's University
• Oct. 2005, Mar. 2007, Associate Professor, Faculty of Science, Nara Women's University
• Apr. 1999, Sep. 2005, Research Scientist, RIKEN (The Institute of Physical and Chemical Research)

Education

• 1997, Tohoku University, Graduate School, Division of Natural Science, Department of Biology
• 1992, Tohoku University, Faculty of Science, Department of Biology

Teaching Experience

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• Graduation Research 2 (Environmental Sciences), Nara Women's University
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• Graduation Research 1 (Environmental Sciences), Nara Women's University
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• Special Course in Environmental Responses of Plants, Nara Women's University
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• Graduation Research 2, Nara Women's University
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• Experiments in Biology (C), Nara Women's University
• Experiments in Biology (B), Nara Women's University
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• Experiments in Biology (A), Nara Women's University
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• Graduation Research 1, Nara Women's University
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• Graduation Research 4, Nara Women's University
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• Basic Practical Course in Biology and Environmental Science IIA, Nara Women's University
• Molecular and Cellular Engineering, Nara Women's University
• Mar. 2023
• Advanced Experimental Course II, Nara Women's University
• Seminar in Biological Science II, Nara Women's University
• Essential Genetics, Nara Women's University
• Mar. 2023
• Special Course in Biological Science 4, Nara Women's University
• Special Course in Biological Science 1, Nara Women's University
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Association Memberships

• The Botanical Society of Japan
• The Japanese Society of Plant Physiologists

Ⅱ．研究活動実績

Published Papers

• Refereed, Plant ＆ Cell Physiology, Inhibition of Pre-mRNA Splicing Promotes Root Hair Development in Arabidopsis thaliana, Miku Ishizawa; Kayo Hashimoto; Misato Ohtani; Ryosuke Sano; Yukio Kurihara; Hiroaki Kusano; Taku Demura; Minami Matsui; Kumi Sato-Nara, Aug. 2019, Scientific journal
• Refereed, Plant and Cell Physiology, Oxford University Press, Functionally Diversified Members of the MIR165/6 Gene Family Regulate Ovule Morphogenesis in Arabidopsis thaliana, Kayo Hashimoto; Shunsuke Miyashima; Kumi Sato-Nara; Toshihiro Yamada; Keiji Nakajima, The ovules of flowering plants consist of a central embryo sac and surrounding layers of the inner and outer integument. As these structural units eventually give rise to the embryo/endosperm and seed coat, respectively, a precisely organized ovule structure is essential for successful fertilization and seed production. In Arabidopsis thaliana, correct ovule patterning depends on the restricted expression of the CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-ZIP III) gene PHABULOSA (PHB) in the apical region of the incipient inner integument, which in turn is regulated via post-transcriptional suppression by miR165 and miR166 (miR165/6) derived from multiple MIR165/6 genes. While a common subset of MIR165/6 genes regulate PHB expression in the root meristem, leaf primordium and embryo, it is unknown whether the same MIR165/6 subset also regulate PHB expression during ovule development. Furthermore, it is unclear where in the ovule primordia miR165/6 are produced. Here, we show that a distinct set of MIR165/6 genes that are highly expressed in the small regions of early ovule primordia restrict the PHB expression domain to promote integument formation. MIR165/6 genes that function in ovule development are phylogenetically distinct from those acting in roots and leaf primordia. Taken together, our data suggest that members of the MIR165/6 gene family are diversified in their expression capacity to establish elaborate PHB expression patterns depending on the developmental context, thereby allowing HD-ZIP III transcription factors to regulate multiple aspects of plant development., 01 May 2018, 59, 5, 1017, 1026, Scientific journal, 10.1093/pcp/pcy042

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• Refereed, Plants, MDPI AG, Accumulation of TIP2;2 aquaporin during dark adaptation is partially phyA dependent in roots of Arabidopsis seedlings, Yumi Uenishi; Yukari Nakabayashi; Ayako Tsuchihira; Mari Takusagawa; Kayo Hashimoto; Masayoshi Maeshima; Kumi Sato-Nara, Light regulates the expression and function of aquaporins, which are involved in water and solute transport. In Arabidopsis thaliana, mRNA levels of one of the aquaporin genes, TIP2 2, increase during dark adaptation and decrease under far-red light illumination, but the effects of light at the protein level and on the mechanism of light regulation remain unknown. Numerous studies have described the light regulation of aquaporin genes, but none have identified the regulatory mechanisms behind this regulation via specific photoreceptor signaling. In this paper, we focus on the role of phytochrome A (phyA) signaling in the regulation of the TIP2 2 protein. We generated Arabidopsis transgenic plants expressing a TIP2 2-GFP fusion protein driven by its own promoter, and showed several differences in TIP2 2 behavior between wild type and the phyA mutant. Fluorescence of TIP2 2-GFP protein in the endodermis of roots in the wild-type seedlings increased during dark adaptation, but not in the phyA mutant. The amount of the TIP2 2-GFP protein in wild-type seedlings decreased rapidly under far-red light illumination, and a delay in reduction of TIP2 2-GFP was observed in the phyA mutant. Our results imply that phyA, cooperating with other photoreceptors, modulates the level of TIP2 2 in Arabidopsis roots., 01 Mar. 2014, 3, 1, 177, 195, Scientific journal, 10.3390/plants3010177

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• Refereed, Plant Signaling and Behavior, Diurnal changes in shoot water dynamics are synchronized with hypocotyl elongation in Arabidopsis thaliana, Haruki Ishikawa; Kumi Sato-Nara; Tomoyuki Takase; Hitoshi Suzuki, We recently demonstrated the circadian clock modulated water dynamics in the roots of a small model plant, Arabidopsis thaliana, by the Nuclear Magnetic Resonance (NMR) microimaging technique. Our developed technique was able to visualize the water distribution that depended on differences in the 1H signal among region in the shoot, such as the shoot apex, the hypocotyl and the root shoot junction. Water content in the shoot increased during periods of light in comparison with dark periods, and continued through the early stage of seedling growth until the dark period. When the water content changed, elongation and/or movement occurred in the hypocotyl, and these events were synchronized. The water dynamics of the shoot also displayed an opposite phase with the root water dynamics. © 2013 Landes Bioscience., Mar. 2013, 8, 3, e23250.2, Scientific journal, 10.4161/psb.23250

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• Refereed, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, A Comprehensive Expression Analysis of the Arabidopsis MICRORNA165/6 Gene Family during Embryogenesis Reveals a Conserved Role in Meristem Specification and a Non-Cell-Autonomous Function, Shunsuke Miyashima; Minami Honda; Kayo Hashimoto; Kiyoshi Tatematsu; Takashi Hashimoto; Kumi Sato-Nara; Kiyotaka Okada; Keiji Nakajima, One of the most fundamental events in plant ontogeny is the specification of the shoot and root apical meristem (SAM and RAM) in embryogenesis. In Arabidopsis, the restricted expression of class III homeodomain leucine zipper (HD-ZIP III) transcription factors (TFs) at the central-apical domain of early embryos is required for the correct specification of the SAM and RAM. Because the expression of HD-ZIP III TFs is suppressed by microRNA165/166 (miR165/6), elucidation of the sites of miR165/6 production and their activity range is a key to understanding the molecular basis of SAM and RAM specification in embryogenesis. Here, we present a comprehensive reporter analysis of all nine Arabidopsis MICRORNA165/166 (MIR165/6) genes during embryogenesis. We show that five MIR165/6 genes are transcribed in a largely conserved pattern in embryos, with their expression being preferentially focused at the basal-peripheral region of embryos. Our analysis also indicated that MIR165/6 transcription does not depend on SCARECROW (SCR) function in early embryos, in contrast to its requirement in post-embryonic roots. Furthermore, by observing the expression pattern of the miR-resistant PHBmu-GFP (green fluorescent protein) reporter, in either the presence or absence of the MIR165Amu transgene, which targets PHBmu-GFP, we obtained data that indicate a non-cell-autonomous function for miR165 in early embryos. These results suggest that miR165, and possibly miR166 as well, has the capacity to act as a positional cue from the basal-peripheral region of early embryos, and remotely controls SAM and RAM specification with their non-cell-autonomous function., Mar. 2013, 54, 3, 375, 384, Scientific journal, 10.1093/pcp/pcs188

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• Refereed, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, The Circadian Clock Modulates Water Dynamics and Aquaporin Expression in Arabidopsis Roots, Tomoyuki Takase; Haruki Ishikawa; Haruko Murakami; Jun Kikuchi; Kumi Sato-Nara; Hitoshi Suzuki, We have developed a plant growth system to analyze water dynamics in the roots of a small model plant, Arabidopsis thaliana, by nuclear magnetic resonance (NMR) microscopic imaging. Using the two-dimensional slice technique, we obtained a series of images with high signal-to-noise ratio indicating the water distribution in the root. To demonstrate light regulation of water transport in the root and involvement of aquaporin gene expression, we visualized the distribution of water in Arabidopsis roots under various light conditions and compared the data with the expression profiles of two aquaporin genes. (1)H-NMR imaging revealed that water content in Arabidopsis roots is lower in the light than in the dark. This diurnal variation in water content was clearly observed in the basal zone of the root. In addition, an autonomous rhythm of water dynamics was observed under continuous light (LL) and darkness (DD). However, the circadian oscillation in water dynamics was obscured in the early-flowering 3 (elf3) mutant under LL. The expression of both the aquaporin genes, AtPIP1;2 and AtPIP2;1, oscillated with the circadian rhythm under LL conditions in wild-type seedlings, but not in the elf3 mutant. These results demonstrate the advantages of our technique for monitoring water dynamics in roots of living Arabidopsis seedlings, and suggest that the circadian clock modulates water dynamics and aquaporin expression., Feb. 2011, 52, 2, 373, 383, Scientific journal, 10.1093/pcp/pcq198

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• Refereed, PLANT CELL AND ENVIRONMENT, BLACKWELL PUBLISHING LTD, Identification of genes regulated by dark adaptation and far-red light illumination in roots of Arabidopsis thaliana, K Sato-Nara; A Nagasaka; H Yamashita; J Ishida; A Enju; M Seki; K Shinozaki; H Suzuki, Roots in the soil are illuminated by far-red (FR) light passed through plant tissues in the daytime, and are in complete darkness at night. To evaluate whether gene expression of roots is affected by a dark-FR light cycle, gene expression profiles were analysed for dark-adapted versus light-grown plants and for FR light-illuminated versus dark-adapted plants using the RIKEN Arabidopsis full-length cDNA microarray (containing approximately 7000 independent, full-length cDNA groups). Among candidate dark- and FR-regulated genes, several were further analysed. Eleven dark-inducible and five dark-repressed genes were characterized. Almost all the dark-inducible and -repressed genes were oppositely regulated by FR light illumination. The functions of dark- and FR-responsive genes and the significance of FR light-regulated gene expression in roots under ground are discussed., Nov. 2004, 27, 11, 1387, 1394, Scientific journal
• Refereed, PLANTA, SPRINGER-VERLAG, Expression of photosynthesis-related genes and their regulation by light during somatic embryogenesis in Daucus carota, K Sato-Nara; T Demura; H Fukuda, To clarify the spatial and temporal pattern of gene expression for photosynthesis-associated proteins during somatic embryogenesis in Daucus carota L., the localization of mRNAs for three genes, rbcL, Lhcb and por, was examined in dark-grown and light-irradiated somatic embryos by in situ hybridization. The three mRNAs were expressed in common in the mesophyll precursor cells of light-irradiated embryos at the late torpedo and plantlet stages, but characteristic expression patterns of each photosynthesis-related gene were also observed. Expression of rbcL mRNA first occurred throughout the embryo but gradually became localized in the mesophyll precursor cells and cortex during early embryogenesis. Localization of Lhcb mRNA in the mesophyll precursor cells and shoot apical meristem became clear in the early torpedo stage. Expression of Lhcb mRNA was not affected by light during early embryogenesis, but could be induced by light in the torpedo stage, suggesting that light-inducible expression of Lhcb mRNA arises within the torpedo stage. At the late torpedo stage, clear localization of por mRNA started in mesophyll precursor cells of the cotyledon in light-irradiated embryos. Greening potency of the embryo also appeared first at this stage. Therefore, greening and initial differentiation of photosynthetic tissues during somatic embryogenesis seem to be associated with coordinated expression of mRNA for rbcL, Lhcb and por in late torpedo-shaped embryos., May 2004, 219, 1, 23, 31, Scientific journal, 10.1007/s00425-003-1201-6

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• Refereed, JOURNAL OF PLANT RESEARCH, SPRINGER-VERLAG TOKYO, Sugar-induced adventitious roots in Arabidopsis seedlings, F Takahashi; K Sato-Nara; K Kobayashi; M Suzuki; H Suzuki, The effects of sugars on root growth and on development of adventitious roots were analyzed in Arabidopsis thaliana. Seeds were sown on agar plates containing 0.0-5.0% sugars and placed vertically in darkness (DD) or under long day (LD,16 h : 8 h) conditions, so that the seedlings were constantly attached to the agar medium. In the sucrose-supplemented medium, seedlings showed sustained growth in both DD and LD. However, only dark-grown seedlings developed adventitious roots from the elongated hypocotyl. The adventitious roots began to develop 5 days after imbibition and increased in number until day 11. They could, however, be initiated at any position along the hypocotyl, near the cotyledon or the primary root. They were initiated in the pericycle in the same manner as ordinary lateral roots. Sucrose, glucose and fructose greatly stimulated the induction of adventitious roots, but mannose or sorbitol did not. Sucrose at concentrations of 0.5-2.0% was most effective in inducing adventitious roots, although 5.0% sucrose suppressed induction. Direct contact of the hypocotyl with the sugar-supplemented agar medium was indispensable for the induction of adventitious roots., Apr. 2003, 116, 2, 83, 91, Scientific journal, 10.1007/s10265-002-0074-2

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• Refereed, JOURNAL OF EXPERIMENTAL BOTANY, OXFORD UNIV PRESS, Detection of ethylene receptor protein Cm-ERS1 during fruit development in melon (Cucumis melo L.), H Takahashi; T Kobayashi; K Sato-Nara; K Tomita; H Ezura, Antibodies against melon ethylene receptor, Cm-ERS1 was prepared. Cm-ERS1 protein formed a disulphide-linked homodimer and it was present in microsomal membranes but not in soluble fractions. Cm-ERS1 protein was present at high levels in melon fruit during early developmental stages. This transition pattern was also observed in another melon cultivar., Mar. 2002, 53, 368, 415, 422, Scientific journal
• Refereed, PLANTA, SPRINGER-VERLAG, The rates of deceleration of nuclear and organellar DNA syntheses differ in the progenitor cells of the apical meristems during carrot somatic embryogenesis, K Sato-Nara; H Fukuda, The synthesis of DNA in nuclei and organellar nucleoids at the various stages of somatic embryogenesis in carrot (Daucus carota L. cv. Kurodagosun) was analyzed using anti-5-bromo-2'-deoxyuridine (BrdU) immunofluorescence microscopy. The active syntheses of both nuclear and organellar DNA started in the cells forming the embryo groper 3 d after the initiation of embryogenesis, but not in cells forming suspensor-like cell aggregates. In the early globular embryo, active DNA syntheses were continuously observed in the whole embryo groper, except for the progenitor cells of the root apical meristem (RAM) and shoot apical meristem (SAM). These were recognized as slowly cycling cells with a non-BrdU-labelled nucleus and strongly BrdU-labelled organellar nucleoids. At the heart- and torpedo-shaped embryo stages, both nuclear and organellar DNA syntheses were inactive in the presumptive RAM and SAM. Thus, slowing down of organellar DNA synthesis is not coupled with, but is later than, that of nuclear DNA synthesis in the progenitor cells of the embryonic RAM and SAM. These findings clearly indicate that the timing of DNA synthesis is similar in the progenitor cells of both the RAM and SAM in the early stages of somatic embryogenesis., Sep. 2000, 211, 4, 457, 466, Scientific journal
• Refereed, PLANT PHYSIOLOGY, AMER SOC PLANT PHYSIOLOGISTS, Stage- and tissue-specific expression of ethylene receptor homolog genes during fruit development in muskmelon, K Sato-Nara; K Yuhashi; K Higashi; K Hosoya; M Kubota; H Ezura, We isolated two muskmelon (Cucumis melo) cDNA homologs of the Arabidopsis ethylene receptor genes ETR1 and ERS1 and designated them Cm-ETR1 (C. melo ETR1; accession no. AF054806) and Cm-ETR1 (C. melo ERS1; accession no. AF037368), respectively. Northern analysis revealed that the level of Cm-ERS1 mRNA in the pericarp increased in parallel with the increase in fruit size and then markedly decreased at the end of enlargement. In fully enlarged fruit the level of Cm-ERS1 mRNA was low in all tissues, whereas that of Cm-ETR1 mRNA was very high in the seeds and placenta. During ripening Cm-ERS1 mRNA increased slightly in the pericarp of fruit before the marked increase of Cm-ETR1 mRNA paralleled climacteric ethylene production. These results indicate that both Cm-ETR1 and Cm-ERS1 play specific roles not only in ripening but also in the early development of melon fruit and that they have distinct roles in particular fruit tissues at particular developmental stages., May 1999, 120, 1, 321, 329, Scientific journal
• Not Refereed, Plant Biotechnology, Japanese Society for Plant Cell and Molecular Biology, Ethylene receptors and genetic engineering of ethylene sensitivity in plants, Kumi Sato-Nara; Ken-Ichi Yuhashi; Hiroshi Ezura, Ethylene is a gaseous plant hormone which gives cues to various developmental processes and stress responses in plants. Plants regulate their ethylene sensitivity for programmed development and responses to environmental stresses. After the successful isolation of the Arabidopsis ethylene receptor gene ETR1, ethylene receptor genes have been found in various plant species. Characterization of ethylene receptor genes provides clues to understanding how plants regulate their ethylene sensitivity. This knowledge also gives us potential methods for engineering the ethylene sensitivity of plants. Genetically - engineered plants with reduced ethylene sensitivity will be potential resources for improving crop performance. Moreover, those transgenic plants will provide further information to elucidate the mechanism of how plants regulate their ethylene sensitivity. The recent progress in genetic and protein analyses of ethylene receptors is summarized in this review. The possible strategies for altering the ethylene sensitivity of plants using the ethylene receptor genes are also discussed., 1999, 16, 5, 321, 334, Scientific journal, 10.5511/plantbiotechnology.16.321

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• Refereed, PLANT AND CELL PHYSIOLOGY, JAPANESE SOC PLANT PHYSIOLOGISTS, Purification and characterization of a mitochondrial DNA polymerase from cultured tobacco cells, K Sato; H Fukuda, The mitochondrial DNA polymerase of tobacco BY-2 cells was purified more than 1,200-fold by column chromatography on DEAE cellulose, phosphocellulose, and Affi-Prep heparin. The enzyme was classified as a gamma-type DNA polymerase, in view of the inhibition of its activity by N-ethylmaleimide and dideoxy TTP, the absence of inhibition by aphidicolin and arabinosyl CTP, the stimulation by KCI, and the ability of the enzyme to utilize poly(A)(dT)(12-18) in the presence of Mn2+ ions. The molecular mass of the native mitochondrial DNA polymerase was estimated to be 70-110 kDa by column chromatography on Superose 12. When DNA polymerase activity was analyzed after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the activity that polymerized DNA was observed as a single band of a protein with a molecular mass of approximately 110 kDa. Therefore, the tobacco mitochondrial DNA polymerase appears to consist of a single subunit of 110 kDa., Oct. 1996, 37, 7, 989, 995, Scientific journal

MISC

• Not Refereed, NMRマイクロイメージング研究会講演要旨集, シロイヌナズナを用いた根の水の状態の比較の試み, 長井理香; 福岡美香; 高瀬智敬; 伊達康博; 菊地淳; 石田信昭; 奈良久美, Aug. 2014, 18th, 9, 10
• Not Refereed, 日本植物学会大会研究発表記録, シロイヌナズナの根の水の状態や輸送に異常のある変異体の探索に向けて, 奈良久美; 長井理香; 丹後真奈美; 伊達康博; 石川春樹; 高瀬智之; 菊地淳, 14 Sep. 2012, 76th, 215
• Not Refereed, 時間生物学, 概日時計はシロイヌナズナの根の水分量を調節する, 高瀬智敬; 石川春樹; 村上晴子; 菊地淳; 奈良久美; 鈴木均, 30 Nov. 2011, 17, 2, 129
• Not Refereed, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, Far-red light illumination to shoots of Arabidopsis plants alters gene expression in roots via two distinct pathways, K Sato-Nara; A Nagasaka; H Yamashita; H Ishikawa; H Suzuki, 2006, 47, S123, S123, Summary international conference
• Not Refereed, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, Water distribution in the root of Arabidopsis thaliana and its time dependent change induced by far-red (FR) light, H Ishikawa; K Sato-Nara; H Suzuki, 2005, 46, S72, S72, Summary international conference
• Not Refereed, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, Far-red light illumination to shoots of Arabidopsis plants causes the repression of an aquaporin gene in roots, K Sato-Nara; T Nagata; H Yamashita; H Ishikawa; M Maeshima; H Suzuki, 2005, 46, S72, S72, Summary international conference
• Not Refereed, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, Far-red light responses of water-channel gene expression in the root of Arabidopsis thaliana, A Nagasaka; K Sato-Nara; H Ishikawa; H Yamashita; Q Sun; H Suzuki, 2004, 45, S45, S45, Summary international conference
• Not Refereed, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, Monitoring gene expression pattern in roots under dark adaptation and far-red light illumination using RIKEN Arabidopsis full-length cDNA microarray, K Sato-Nara; H Yamashita; Q Sun; M Seki; K Shinozaki; H Suzuki, 2003, 44, S150, S150, Summary international conference
• 日本植物生理学会年会(Web), Genome-wide alternation of pre-messenger RNA splicing in a light-sensitive root-hair development 1 mutant of Arabidopsis thaliana, 石澤未来; 橋本佳世; 橋本佳世; 大谷美沙都; 佐野亮輔; 栗原志夫; 草野博彰; 出村拓; 松井南; 奈良久美, 2020, 61st
• 日本植物学会大会研究発表記録, pre-mRNAスプライシングの阻害によるシロイヌナズナの根毛形成の促進, 石澤未来; 橋本佳世; 橋本佳世; 大谷美沙都; 草野博彰; 松井南; 奈良久美, 2019, 83rd
• Plant and cell physiology, EXPRESSION OF ETR1/ERS HOMOLOG GENES DURING FRUIT DEVELOPMENT IN MELON (Cucumis melo L. reticulatus), SATO-NARA Kumi; YUHASHI Ken-Ichi; HIGASHI Katsumi; HOSOYA Kazushige; KUBOTA Mitsuru; EZURA Hiroshi, May 1998, 39, S72, S72
• Not Refereed, 日本植物生理学会年会要旨集, 日本植物生理学会, 光によって根毛形成が促進されるシロイヌナズナ突然変異体の単離と解析, 奈良久美; 小林和貴; 正岡詩織; 小林淳子; 中澤美紀; 松井南; 鈴木均, In <I>Arabidopsis</I>, the root of a light-grown plant has a higher density of root hairs than that of a dark-grown plant. Root hair formation is regulated by various factors such as phytochrome and hormone. To understand the molecular mechanism of light promoted root hair formation, we screened <I>Arabidopsis</I> activation tagging lines and isolated a new light-promoted root-hair development mutant, <I>lrh1</I>. By comparing root hair densities of the wild type and the <I>lrh1</I> mutant under continuous light conditions, it was suspected that a mutated gene might induce root hair formation. Mutant seedlings also had shorter roots than wild type, and reduced numbers of lateral roots. A database search revealed that an 11-kilobase sequence adjacent to the location of T-DNA insertion contained two repeated sequences, hundreds of small RNAs, and several proproteins. We expect that <I>lrh1</I> will be a useful tool for discovering novel factors to regulate root hair formation., 15 Mar. 2008, 49th, 0, 268, 660, 10.14841/jspp.2008.0.0660.0

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• 日本植物生理学会年会(Web), Effects of a mutation in the Tonoplast Intrinsic Protein 2;2 (TIP2;2) gene on metabolites in leaves of Arabidopsis thaliana, 本廣夕佳; 土井李里夏; 松本朋子; 菊地淳; 桑形恒男; 半場祐子; 奈良久美, 2023, 64th

Presentations

• Poster presentation, 23 Jul. 2011, 30 Jul. 2011
• Poster presentation, 14 Mar. 2021, 16 Mar. 2021
• Miku Ishizawa; Kayo Hashimoto; Misato Ohtani; Ryosuke Sano; Yukio Kurihara; Hiroaki Kusano; Taku Demura; Minami Matsui; Kumi Sato-Nara, The 61st Annual Meeting of The Japanese Society of Plant Physiologists, Genome-wide alternation of pre-messenger RNA splicing in a light-sensitive root-hair development 1 mutant of Arabidopsis thaliana, 20 Mar. 2020, 19 Mar. 2020, 21 Mar. 2020

  共同研究・競争的資金等URL
• Tomomi Fujita; Ayako Okumura; Ayako Tsuchihira; Masayoshi Maeshima; Maki Katsuhara; Sato-Nara Kumi, The 83th Annual Meeting of the Botanical Society of Japan, 時計因子ELF3は胚軸における水輸送調節にどのように関連しているのか？, Poster presentation, 17 Sep. 2019, False
• Miku Ishizawa; Kayo Hashimoto; Misato Otani; Hiroaki Kusano; Minami Matsui; Kumi Sato-Nara, The 83th Annual Meeting of the Botanical Society of Japan, pre-mRNAスプライシングの阻害によるシロイヌナズナの根毛形成の促進, Poster presentation, 17 Sep. 2019, False
• Yukako Yamanari; Yoshiki Nakahara; Maki Katsuhara; Sato-Nara Kumi, The 83th Annual Meeting of the Botanical Society of Japan, シロイヌナズナ液胞膜型アクアポリンAtTIP2;2の過酸化水素透過性の検討, Poster presentation, 16 Sep. 2019, False
• Miku Ishizawa; Kayo Hashimoto; Misato Otani; Minami Matsui; Kumi Sato-Nara, The 82th Annual Meeting of the Botanical Society of Japan, シロイヌナズナの光によるRNAスプライシングの調節と根毛形成促進との関連性の検討, Poster presentation, Sep. 2018, The Botanical Society of Japan, Hiroshima
• Tomomi Fujita; Ayako Okumura; Maki Katsuhara; Kumi Sato-Nara, The 82th Annual Meeting of the Botanical Society of Japan, シロイヌナズナの根と根細胞の水透過性測定, Poster presentation, Sep. 2018, The Botanical Society of Japan, Hiroshima
• Yukako Yamanari; Yoshiki Nakahara; Maki Katsuhara; Kumi Sato-Nara, The 82th Annual Meeting of the Botanical Society of Japan, Water permeability of tonoplast intrinsic proteins TIP2;2 and TIP2;3 in Arabidopsis thaliana, Poster presentation, Sep. 2018, The Botanical Society of Japan, Hiroshima
• Sato-Nara Kumi; Miki Kato; Yukako Yamanari; Kumi Sato-Nara, The 59th Annual Meeting of The Japanese Society of Plant Physiologists, Promotion of root cell elongation and stress tolerance in a tonoplast intrinsic protein TIP2;2-deficient mutant in Arabidopsis thaliana, Poster presentation, Mar. 2018, Sapporo, False
• Sato-Nara Kumi; Yoshikawa Miho; Komoda Masako; Ayako Tsuchihira; Masayoshi Maeshima; Kumi Sato-Nara, The 81st Annual Meeting of the Botanical Society of Japan, シロイヌナズナの原形質膜アクアポリン遺伝子PIP2;3の高温誘導を増大させる光の波長の検討, Poster presentation, Sep. 2017, The Botanical Society of Japan, Noda, Chiba
• Sato-Nara Kumi; Miki Kato; Kumi Sato-Nara, The 80th Annual Meeting of the Botanical Society of Japan, シロイヌナズナのアクアポリンtip2;2及びtip2;3変異体のストレス応答の解析, Poster presentation, Sep. 2016, The Botanical Society of Japan, Okinawa
• Sato-Nara Kumi; Ayako Okumura; Maki Katsuhara; Toshiyuki Kaneko; Kumi Sato-Nara, The 80th Annual Meeting of the Botanical Society of Japan, シロイヌナズナ概日時計変異体elf3の根の水透過性, Poster presentation, Sep. 2016, The Botanical Society of Japan, Okinawa
• Sato-Nara Kumi; Chiho Makino; Ayako Tsuchihira; Manami Tango; Masayoshi Maeshima; Kumi Sato-Nara, The 80th Annual Meeting of the Botanical Society of Japan, シロイヌナズナの原形質膜アクアポリンPIP2;1遺伝子の光による発現調節, Poster presentation, Sep. 2016, The Botanical Society of Japan, Okinawa
• Sato-Nara Kumi; Miki Kato; Kumi Sato-Nara, NWU-UL Mini-symposium on Bioscience, Growth analysis of aquaporin mutants deficient in tonoplast intrinsic proteins TIP2;2 and TIP2;3 in Arabidopsis thaliana, Oral presentation, Mar. 2016, Nara, True
• Sato-Nara Kumi; Miki Kato; Kumi Sato-Nara, The 79th Annual Meeting of the Botanical Society of Japan, シロイヌナズナのアクアポリンTIP2;2及びTIP2;3の欠損による成長への影響, Poster presentation, Sep. 2015, The Botanical Society of Japan, Niigata
• Sato-Nara Kumi; Keiko Yamashita; Hiroaki Kusano; Kayo Hashimoto; Miki Nakazawa; Minami Matsui; Kumi Sato-Nara, The 56th Annual Meeting of The Japanese Society of Plant Physiologists, Isolation and characterization of the light-promoted root hair development (lrh1) mutant in Arabidopsis thaliana, Poster presentation, Mar. 2015, False
• Sato-Nara Kumi; Junko Yamaguchi; Ai Miyazaki-Yoshimoto; Kumi Sato-Nara, The 56th Annual Meeting of The Japanese Society of Plant Physiologists, Expression analysis of the Far-red light-inducible PR1-Like (FPL) gene in phyA mutant of Arabidopsis thaliana, Poster presentation, Mar. 2015, 東京都世田谷区（東京農業大学）, False
• Sato-Nara Kumi; Okumura Ayako; Katsuhara Maki; Kaneko Toshiyuki; Sato-Nara Kumi, The 56th Annual Meeting of The Japanese Society of Plant Physiologists, Are phosphorylation of PIP2s aquaporins and water permeability in the Arabidopsis root modulated by the circadian clock?, Poster presentation, Mar. 2015, 東京都世田谷区（東京農業大学）, False
• Sato-Nara Kumi, The 78th Annual Meeting of the Botanical Society of Japan, シロイヌナズナの根の細胞サイズとMRIによる水の状態の解析, Poster presentation, Sep. 2014, The Botanical Society of Japan, Kanagawa, False
• Sato-Nara Kumi, 第１８回ＮＭＲマイクロイメージング研究会, シロイヌナズナを用いた根の水の状態の比較の試み, Poster presentation, Aug. 2014, 石川県金沢市, False
• Sato-Nara Kumi; Rika Nagai; Ayako Tsuchihira; Masayoshi Maeshima; Kumi Sato-Nara, The 55th Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), Is light regulation of aquaporin expression in the shoot of Arabidopsis seedlings involved in modulation of root water dynamics?, Poster presentation, Mar. 2014, The Japanese Society of Plant Physiologists (JSPP), Toyama, False
• Sato-Nara Kumi, The 77th Annual Meeting of the Botanical Society of Japan, シロイヌナズナの根の細胞サイズの光と糖による調節, Poster presentation, Sep. 2013, 日本植物学会, 北海道札幌市, False
• Sato-Nara Kumi, The 76th Annual Meeting of the Botanical Society of Japan, 花器官において発現するMIR165/166遺伝子の探索, Poster presentation, Sep. 2012, 日本植物学会, 兵庫県姫路市, False
• Sato-Nara Kumi, The 76th Annual Meeting of the Botanical Society of Japan, シロイヌナズナの根の水の状態や輸送に異常のある変異体の探索に向けて, Poster presentation, Sep. 2012, 日本植物学会, 兵庫県立大学姫路書写キャンパス（兵庫県姫路市）, False
• Sato-Nara Kumi; Kayo Hashimoto; Miki Makazawa; Minami Matsui; Kumi Sato-Nara, NWU-UL Mini-symposium on Bioscience, Characterization of MIR166c and MIR166d overexpression mutant in Arabidopsis thaliana, Mar. 2012, Nara, True
• Sato-Nara Kumi, the 53rd Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), Accumulation of aquaporins in the root of phytochrome-A-deficient mutant in Arabidopsis thaliana, Poster presentation, Mar. 2012, False
• Sato-Nara Kumi; Kayo Hashimoto; Miki Nakazawa; Minami Matsui; Kumi Sato-Nara, The 53rd Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), The effects of MIR166d overexpression in gynoecium development, Poster presentation, Mar. 2012, 日本植物生理学会, Kyoto, False
• Sato-Nara Kumi, The 75th Annual Meeting of the Botanical Society of Japan, シロイヌナズナMIR166cおよびMIR166d過剰発現系統における形態異常の原因を探る, Poster presentation, Sep. 2011, False
• Sato-Nara Kumi, 第３回植物アクアポリン研究検討会, シロイヌナズナの概日時計突然変異体を利用したアクアポリンの発現制御機構の研究, Oral presentation, Jun. 2011, False
• Sato-Nara Kumi, 第３回植物アクアポリン研究検討会, フィトクロムAの欠損によるアクアポリンの量や局在への影響を探るための試み, Oral presentation, Jun. 2011, False
• Sato-Nara Kumi, the 52nd Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), シロイヌナズナMIR166cおよびMIR166d過剰発現体の解析, Poster presentation, Mar. 2011, False
• Sato-Nara Kumi, the 52nd Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), シロイヌナズナのEARLY FLOWERING 3 (ELF3)の欠損はアクアポリンの概日調節に影響を与えるのか？, Oral presentation, Mar. 2011, False
• Sato-Nara Kumi, The 74th Annual Meeting of the Botanical Society of Japan, シロイヌナズナのアクアポリン遺伝子の概日リズムによる発現調節, Poster presentation, Sep. 2010, False
• Sato-Nara Kumi, The 74th Annual Meeting of the Botanical Society of Japan, 花茎帯化及び不稔形質を呈するシロイヌナズナ突然変異系統の単離と解析, Poster presentation, Sep. 2010, False
• Sato-Nara Kumi; Haruki Ishikawa; Yumi Uenishi; Tomoyuki Takase; Atsushi Nagasaka; Ayako Tsuchihira; Masayoshi Maeshima; Jun Kikuchi; Hitoshi Suzuki; Kumi Sato-Nara, 21st International Conference on Arabidpsis Research, Involvement of phytochrome A in regulation of water dynamics and aquaporin expression in Arabidopsis roots, Poster presentation, Jun. 2010, True
• Sato-Nara Kumi, the 51st Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), シロイヌナズナの液胞膜アクアポリンTIP2;2の組織局在と暗順応への応答, Oral presentation, Mar. 2010, False
• Sato-Nara Kumi; Yumi Uenishi; Haruki Ishikawa; Tomoyuki Takase; Atsushi Nagasaka; Ayako Tsuchihira; Masayoshi Maeshima; Hitoshi Suzuki; Kumi Sato-Nara, Memorial Symposium for the 25th International Prize for Biology Celebrating Dr. Winslow R. Briggs "Biology of Sensing", Involvement of phytochrome A in regulation of water dynamics and aquaporin expression in Arabidopsis roots, Poster presentation, Dec. 2009, True
• Sato-Nara Kumi, 2009年度日本植物学会近畿支部会, 遠赤色光応答性PR1様遺伝子FPLはエチレンに依存し根の組織特異的に発現する, Oral presentation, Nov. 2009, False
• Sato-Nara Kumi, the 50th Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), シロイヌナズナの遠赤色光誘導遺伝子FPLのエチレン応答, Poster presentation, Mar. 2009, False
• Sato-Nara Kumi, The 72th Annual Meeting of the Botanical Society of Japan, シロイヌナズナの根のアクアポリンの光応答：フィトクロムA突然変異体を用いた解析, Oral presentation, Sep. 2008, False
• Sato-Nara Kumi, the 49th Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), 光によって根毛形成が促進されるシロイヌナズナ突然変異体の単離と解析, Oral presentation, Mar. 2008, False
• Sato-Nara Kumi; Haruki Ishikawa; Kumi Sato-Nara; Ayako Tsuchihira; Masayoshi Maeshima; Atsushi Nagasaka; Hitoshi Suzuki, The 5th International Conference of Aquaporin, Effects of far-red light on water distribution and aquaporin gene expression in the root of Arabidopsis thaliana, Poster presentation, Jul. 2007, True
• Sato-Nara Kumi; Kumi Sato-Nara; Atsushi Nagasaka; Hizuru Yamashita; Haruki Ishikawa; Hitoshi Suzuki, The 5th International Conference of Aquaporin, Involvement of two distinct signaling pathways induced by far-red light with TIP2;2 gene regulation in roots of Arabidopsis, Oral presentation, Jul. 2007, True
• Sato-Nara Kumi; Kumi Sato-Nara; Kazutaka Kobayashi; Junko Kobayashi; Miki Nakazawa; Minami Matsui; Hitoshi Suzuki, Nara Women's University International Symposium－Light Regulation of Plant Growth and Development., Characterizatoin of a light-promoted root-hair development mutant, lrh1, of Arabidopsis., Nominated symposium, Jan. 2007, The assistance office of woman researchers, Nara, True
• Sato-Nara Kumi, 第2回アクアポリン研究討論会, シロイヌナズナTIP2;2遺伝子の遠赤色光による発現制御機構の解析, Oral presentation, Mar. 2006, False
• Sato-Nara Kumi, the 47th Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), シュートへの遠赤色光照射による根の遺伝子発現調節機構の解析, Oral presentation, Mar. 2006, False
• Sato-Nara Kumi, the 46th Annual Meeting of the Japanese Society of Plant Physiologists (JSPP), シュートへの遠赤色光照射による根のアクアポリン遺伝子による発現抑制, Oral presentation, Mar. 2005, False
• Sato-Nara Kumi, 日本植物学会東北支部第17回大会, 光は根の水輸送を調節しているか?, Poster presentation, Dec. 2004, False
• Yukako Yamanari; Yoshiki Nakahara; Hina Fujimoto; Yuka Motohiro; Tsuneo Kuwagata; Yuko T. Hanba; Maki Katsuhara; Kumi Sato-Nara, The 62nd Annual Meeting of the Japanese Society of Plant Physiologists, Functional analysis of tonoplast intrinsic protein AtTIP2;2 in Arabidopsis thaliana, Poster presentation, 16 Mar. 2021, 14 Mar. 2021, 16 Mar. 2021

  共同研究・競争的資金等URL
• Hina Fujimoto; Yuka Motohiro; Tsuneo Kuwagata; Yuko T. Hanba; Kumi Sato-Nara, The 63rd Annual Meeting of the Japanese Society of Plant Physiologists, Osmotic responses in;protoplasts of mutant cells lacking tonoplast intrinsic;protein AtTIP;in Arabidopsis thaliana, Poster presentation, 24 Mar. 2022, 22 Mar. 2022, 24 Mar. 2022

  共同研究・競争的資金等URL
• 冨田麟太郎; 半場祐子; 奈良久美, The 69th Annual Meeting of the Ecological Society of Japan, Involvement of Arabidopsis thaliana Aquaporin Tip2;2 in Photosynthesis under High Humidity Conditions, Poster presentation, 14 Mar. 2022, 14 Mar. 2022, 19 Mar. 2022

  共同研究・競争的資金等URL
• 奈良久美; 海老原 諒子; 松原 知咲希, 日本植物学会第86回大会, シロイヌナズナのスプライシング因⼦p14-1の花器官発⽣における役割について, Poster presentation, 15 Sep. 2022, 15 Sep. 2022, 19 Sep. 2022
• Rintaro TOMITA; Kumi NARA; Yuko HANBA, 70th Annual Meeting of Ecological Society of Japan, effect of Arabidopsis aquaporins in photosynthesis in response to high humidity stress, Poster presentation, 17 Mar. 2023, 17 Mar. 2023, 21 Mar. 2023

  共同研究・競争的資金等URL
• Yuka Motohiro; Doi Ririka; Tomoko Matsumoto; Jun Kikuchi; Tsuneo Kuwagata; Yuko T. Hanba; Kumi Sato-Nara, The 64th Annual Meeting of the Japanese Society of Plant Physiologists, Effects of a mutation in the Tonoplast Intrinsic Protein 2;2 (TIP2;2) gene on metabolites in leaves of Arabidopsis thaliana, Poster presentation, 13 Mar. 2023, 10 Mar. 2023, 17 Mar. 2023

  共同研究・競争的資金等URL
• 橘実来; 奈良久美, 日本植物学会 第87回大会, シロイヌナズナの実生発達に対するアクアポリンの貢献度を探る, 04 Sep. 2023, 04 Sep. 2023, 09 Sep. 2023

  共同研究・競争的資金等URL

Works

• 遠赤色光EEFL照明によるシロイヌナズナの栽培試験研究, Oct. 2010, Mar. 2011
• Cultivation test of Arabidopsis thaliana under far-red light illumination using an EEFL system, Oct. 2010, Mar. 2011

Awards

• Rising Sun Award, 16th Conference of the International Plant Growth Substances Association (IPGSA), Kumi Sato-Nara, 1998

Industrial Property Rights

• Patent right, Infrared observation and micromanipulation device, Sato-Nara Kumi, 理化学研究所, 200212, 24 Dec. 2002, 3549107
• Patent right, ethylene-low-sensitive plants, Sato-Nara Kumi, 茨城県, 199906, 08 Jun. 1999, 3030015, Apr. 2000

Research Projects

• Apr. 2019, Mar. 2023, 都市樹木における環境適正診断法の確立：アクアポリンによる気孔応答評価の導入, Yuko T. Hanba, Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), 0, 0, 0, Competitive research funding, rm:presentations;rm:presentations;rm:presentations;rm:presentations;rm:presentations

  対象リソースIRI
• Apr. 2015, Mar. 2019, Principal investigator, 環境や時計によるシロイヌナズナの水輸送調節機構の研究, Kumi Sato-Nara, Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), 0, 0, 0, Competitive research funding, rm:presentations;rm:presentations

  対象リソースIRI
• Apr. 2012, Mar. 2014, Principal investigator, シロイヌナズナの根の水輸送調節の分子メカニズムを探る：MRIを用いた挑戦, Kumi Sato-Nara, Japan Society for the Promotion of Science, Grant-in-Aid for challenging Exploratory Research, 0, 0, 0, Competitive research funding
• 2012, 2013, Principal investigator, Floral morphogenesis and miR166, Kumi Sato-Nara, Nara Women's University Intramural Grant for Project Research, 0, 0, 0, Competitive research funding
• 2011, 2012, Principal investigator, 植物における根の形態形成・機能の光による調節メカニズムの研究, Kumi Sato-Nara, Nara Women's University Intramural Grant for Project Research A, 0, 0, 0, Competitive research funding
• 2008, 2009, Principal investigator, シロイヌナズナのアクアポリンTIP2;2の遠赤色光による発現制御機構の解析, Kumi Sato-Nara, Nara Women's University Intramural Grant for Project Research A, 0, 0, 0, Competitive research funding
• 2007, 2008, Principal investigator, Light promotion of root hair development, Kumi Sato-Nara, Nara Women's University Intramural Grant for Project Research A, 0, 0, 0, Competitive research funding
• 2006, 2007, Principal investigator, Far-red light regulation of root FPL gene expression in Arabidopsis thaliana, Kumi Sato-Nara, Nara Women's University Intramural Grant for Project Research, 0, 0, 0, Competitive research funding
• Apr. 2003, Mar. 2006, Principal investigator, シロイヌナズナの根における遠赤色光応答の分子機構の研究, Kumi Sato-Nara, Grant-in-Aid for Young Scientists (B), 0, 0, 0, Competitive research funding
• Apr. 2017, Mar. 2020, Principal investigator, Analysis of the water and hydrogen peroxide permeability of AtTIP2;2 in Arabidopsis thaliana, Yukako Yamanari; Yoshiki Nakahara; Maki Katsuhara; Kumi Sato-Nara, Institute of Plant Science and Resources, Okayama University, The Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University, Nara Women's University, rm:presentations

  対象リソースIRI
• 01 Apr. 2019, 31 Mar. 2024, 19H04281, Coinvestigator
• Apr. 2019, Mar. 2023
• Apr. 2019, Mar. 2023
• Apr. 2017, Mar. 2020, Principal investigator
• Apr. 2017, Mar. 2020, Principal investigator
• Apr. 2015, Mar. 2019, Principal investigator
• Apr. 2015, Mar. 2019, Principal investigator
• Apr. 2015, Mar. 2019, Principal investigator
• Grant-in-Aid for Scientific Research (B), 01 Apr. 2023, 31 Mar. 2027, 23H03552, Establishment of a new evaluation method for urban tree function based on the relationship between carbon stable isotope ratios and aquaporins, 富田 祐子; 馬場 啓一; 久米 篤; 奈良 久美; 北島 佐紀人, Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Kyoto Institute of Technology, 17290000, 13300000, 3990000, kaken;rm:presentations

  対象リソースIRI

Ⅲ．社会連携活動実績

１．公的団体の委員等（審議会、国家試験委員、他大学評価委員，科研費審査委員等）

• The Japanese Society of Plant Physiologists, International Committee Members, Jan. 2020, Dec. 2023, Society
• The Japanese Society of Plant Physiologists, Treasurers, 2009, 2010, Society
• Dec. 2015, 9999, Autonomy
• Oct. 2014, 9999, Autonomy
• 2020, 2021
• 01 Dec. 2015, 31 Dec. 2021
• 01 Oct. 2014, 31 Oct. 2021
• 2020, 2022
• 01 Dec. 2020, 30 Nov. 2022
• 26 Dec. 2021, 25 Dec. 2023
• 26 Dec. 2019, 25 Dec. 2021
• 2020, 9999
• 2020, 9999
• 2009, 2010
• 01 Dec. 2020, 30 Nov. 2022