日本語
 Faculty
 Division of Natural Sciences
 Research Group of Chemistry
Professor
NAKAZAWA   Takashi

Other faculty or department / position
Faculty of Science Department of Chemistry,Biology, and Environmental Sciences Courses of Chemistry
Faculty of Science Department of Chemistry,Biology, and Environmental Sciences Courses of Environmental Sciences
Graduate School of Humanities and Sciences Master's Course Department of Chemistry
Graduate School of Humanities and Sciences Doctoral Course School of Natural Science and Ecological Awareness

Career
National Institute for Physiological Science, Okazaki National Research Institute, Lecturer; Research fellow  1983/04-1983/12 
Research Assistant, Faculty of Science, Nara Women's University  1984/01-1989/03 
Lecturer, Faculty of Science, Nara Women's University  1989/04-1993/03 
Associate Professor, Faculty of Science, Nara Women's University  1993/04-2007/03/31 
Visiting Lecturer, Faculty of Education, Nara University of Education  1997/04-Present 
奈良女子大学・理学部・准教授  2007/04/01-2008/03/31 
奈良女子大学・理学部・教授  2008/04/01-Present 

Graduate school
Osaka University    Graduate School, Division of Natural Science  有機化学  1982  Completed  Domestic   

School
Osaka University  Faculty of Science  化学  1977    Domestic   

Academic degrees
Doctor of Science  Osaka University   

Current state of research and teaching activities
My research interests include proteomics related to the development of chemical modification techniques of proteins for facilitating the structural analysis using MALDI-MS. I also devoted to analysis, elucidation, and prediction of protein structure in three dimensions.   

Current research field
Living Organism Molecular Science 
Structural Biochemistry 
Organic Chemistry 
Archeology 
Analytical Chemistry 
Cultural Asset Science 
Biophysics 

Research keywords
Protein Science, Proteomics, Organic Chemistry, Mass Spectrometry, Nuclear Magnetic Resonance, Archaeological Science. 

Subject of research
Alteration of substrate specificity of serine proteases by chemical modification  serine protease chemical modification substrate recognition  1988- 
Development of the method for the C-terminal activation of proteins  protein, C-terminus, oxazolone  1999-2009 
Development of Chemical Methods for Proteomics Studies  Mass Spectrometry de novo sequencing Proteomics  1999- 
Structural research of collagen model peptides  collagen peptide protein folding  2000- 
Analysis of dynamic structure of proteins using histidine residues to be probing micro-environment.  histidine, micro-environment, dynamics, protein, mass spectrometry.  2007-  ヒスチジンC2-プロトンのH-D交換反応を質量分析法により追跡し、その速度論的パラメーターからタンパク質中の各ヒスチジン固有のpKa値と交換反応速度定数を求める。
Development of the method for the specific identification of animals based on amino acid sequencing of collagen in archaeological samples using mass spectrometry.  archaeology, proteomics, amino acid sequencing, collagen, mass spectrometry.  2008- 
Identification of species of animal bones excavated from the Neolithic and Paleolithic sites by the amino acid sequencing of collagen.  collagen, animal bone, the Neolithic, the Paleolithic, mass spectrometry, amino acid sequencing, protein archaeology  2017-2021 
Synthesis of dehydroamino-acid-containing peptides  dehydroamino acid,peptide chemistry,unnatural amino acid   
ab initio prediction of tertiary structure of peptides and proteins by energy minimization  energy minimization,Monte Carlo simulated annealing,tertiary-structure prediction of proteins   

Proposed theme of joint or funded research
Heavy-atom Labeling of Proteins for High-Resolution X-Ray Analyses    Wish to undertake joint research with industry and other organizations including private sector.  Technical consultation,Commisioned research,Joint research   
Functional Alteration and Analysis of Proteins by Chemical Modification    Joint research with other research organizations such as universities.     
Protein Archaeology  遺跡からの出土品や文化財に含まれるタンパク質の分析により、素材や原料生物の特定を行う。その結果をもとに、古代の歴史、文化、技術について研究を行う。  Wish to undertake joint research with industry and other organizations including private sector.  Technical consultation,Commisioned research,Joint research  代表的な考古学的タンパク質として、膠のコラーゲン、絹のフィブロインとセリシンについて研究しています。 
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Achievements of joint or funded research
Physicochemical studies on the mechanism of the collagen triple helix    -Present  Domestic joint research within Japan 
Computational prediction of tertiary structure of proteins based on first principles&0d0a;&0d0a;    -Present  International joint research 
Computational prediction of tertiary structure of proteins based on first principles&0d0a;&0d0a;    -Present  Research commisioned by company 
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Books
Sericulture of ancient Japan as revealed by silk excavated from the ruin of Makimuku  2013/09/25  Chemistry  12-16    0451-1964   
生命元素事典  2006/03  オーム社  407-411  矢野重信     
生体成分のFD-Massによる分析  1982/06  講談社  263-273  崎山文夫     
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Papers
Protein archaeology: study of animal bones from the Paleolithic sites.  考古学ジャーナル  Academic Journal  Only  708, 35-38  2018/02    0454-1634     
X-Ray snapshots of a pyridoxal enzyme: a catalytic mechanism involving concerted [1,5]-hydrogen sigmatropy in methionine γ-lyase  Scientific Reports  Academic Journal  Joint  7, 1-10  2017/07  Dan Sato, Tomoo Shiba, Tsuyoshi Karaki, Wataru Yamagata, Tomoyoshi Nozaki, Takashi Nakazawa, Shigeharu Harada    doi:10.1038/s41598-017-05032-6   
History of Japannese sericulture in view of the species of silk moth - Literary records of domesticated and wild Japanese silk moths in ancient Japan -  古代学  Bulletin of Universities and Institutes  Only  9, 1-10  2017/03  Takashi Nakazawa  2187-4093    Repository 
Characterization of binding media in Egyptian Romano portraits using enzyme-linked immunosorbant assay and mass spectrometry  e-Preservation Science (e-PS)  Academic Journal  Joint  11, 76-83  2014/09  Joy Mazurek, Marie Svoboda, Jeffrey Maish, Kazuki Kawahara, Shunsuke Fukakusa, Takashi Nakazawa, Yoko Taniguchi  1854-3928     
Imidazole C-2 hydrogen/deuterium exchange reaction at histidine for probing protein structure and function with MALDI mass spectrometry  Biochemistry  Academic Journal  Joint  53/ 11, 1818-1826  2014/03  Naoka Hayashi, Hiroki Kuyama, Chihiro Nakajima, Kazuki Kawahara, Masaru Miyagi, Osamu Nishimura, Hisayuki Matsuo    10.1021/bi401260f   
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Research presentations
Conference presentation  How Collagen Molecules Get Older? - A Strange Life of Collagen -  Workshop 03: Art and Cultural Heritage Mass Spectrometry In 64th ASMS Conference on Mass Spectrometry and Allied Topics  2016/06/07 
Conference presentation  Mass Spectrometry of Collagen and Casein in the Remains of the 5th to 7th Century Bamiyan Buddhas  64th ASMS Conference on Mass Spectrometry and Allied Topics  2016/06/06 
Conference presentation  Mass spectrometry of collagen preserved in archaeological specimens including human bones  Proceedings of the 63rd ASMS Conference on Mass Spectrometry and Allied Topics.  2015/06/01 
Symposium  エジプトの膠  シンポジウム:西アジア発掘と関連科学  2015/03/28 
Symposium  骨と皮のコラーゲン  シンポジウム:皮と膠  2014/07/19 
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Intellectual property rights
Patent  Method for C-terminal modification, C-terminal immobilization, and analysis of protein or peptide  特願2005-307831  2005/10         
Patent  Method for derivatizing protein or peptide to sulfonic acid derivative (European Patent)  EP20040024436 20041013  2004/10  EP1561755  2005/08     
Patent  Method for derivatizing protein or peptide to sulfonic acid groups  G103073US  2004/10  US20050084927  2005/04  US 7,579,165 B2   
Patent  Method for determining amino acid sequence of a peptide  G103015US  2004/03  US20040180381  2004/09     
Patent  Novel metal complex and method for determining amino acid sequence of protein (US Patent)  US20030614324 20030708  2003/07    2004/02  US2004029181   
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Alloted class
奈良女子大の化学 
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Memberships of academic societies
The Japan Society for Analytical Chemistry 2013/07/01-Present  Domestic
近畿支部幹事 2013/08/01-Present
Protein Society of Japan 2012/11/01-Present  Domestic
The Mass Spectrometry Society of Japan 2012/01/10-Present  Domestic
日本文化財科学会 2010-Present  Domestic
Biochemical Society of Japan 2010-Present  Domestic
Fellow 2012/04/01-Present
有機合成協会 2004-2010/03/31  Domestic
幹事(関西支部) 2004/01-2011/12/31
American Society for Mass Spectrometry 2002/02/01-Present  Overseas
American Chemical Society 2000/12/23-Present  Overseas
Chemical Society of Japan 1984-Present  Domestic
代議員 2011/10/29-2012/10/26